CN101120684B

CN101120684B - Method for preparing biological preparation for decreasing organic phosphorous pesticide residue and promoting growth

Info

Publication number: CN101120684B
Application number: CN200510129937XA
Authority: CN
Inventors: 汪东风; 罗轶; 孙继鹏
Original assignee: Ocean University of China
Current assignee: Ocean University of China
Priority date: 2004-04-22
Filing date: 2004-04-22
Publication date: 2011-03-30
Anticipated expiration: 2024-04-22
Also published as: CN101120684A

Abstract

A preparation for a biological agent reducing the residual organophosphorus pesticide and promoting the growth of the crops is to use the concentrated waste solution containing the tea polysaccharides after used for processing the tea polyphenol and the theine of the tea to adjust the pH value directly or after added with rare earth, and then the solution is concentrated to be a liquid biological agent. The biological agent of the present invention also can be prepared by adding the solid tea polysaccharides after dissolved into an agricultural nitrate rare earth. The biological agent of the present invention can make the degradation rate of the organophosphorus pesticide reach more than 80 percent and make the yield-increasing rate of the crops reach 3 to 20 percent after several days, which is a natural agent or an agent containing little rare earth, thereby greatly reducing the application quantity of the rare earth and improving the security of the agricultural rare earth; moreover, the present invention enables the abundant tea resource in China to be adequately used with a high value, which has no waste gas and no waste water to pollute the environment; moreover, the raw materials are easy to get, the investment is small, which conforms to the technical modification requirement of the prior tea polyphenol factory to improve the benefit.

Description

A kind of preparation method of biological preparation decreasing organic phosphorous pesticide residue and promoting growth

Technical field

The present invention relates to a kind of preparation method of biologic product of decreasing organic phosphorous pesticide residue and promoting growth.

Background technology

China has a large population in a small area, and how to guarantee that size of food safety and quality safety are long-term and difficult task.With regard to plant resource food, no matter be large grain-production, or the production of vegetable and fruit, their growth busy season is also mostly to be that the busy season takes place in insect pest.Present stage, the peasant also needed to use the chemical pesticide control insect pest in order to improve their output and quality.Along with the complete prohibition use and the pyrethroid pesticide of organo-chlorine pesticide easily makes insect develop immunity to drugs, organophosphorous pesticide has accounted for 70% of insecticide total amount at present.But long-term large-scale production and use organophosphorous pesticide not only causes severe contamination to environment, residual in food and feed people and cultivated animals is also seriously influenced.And the superseded use or reach in food hygienic standard of construction, biopesticide and organophosphorous pesticide development of substitutes and some organophosphorous pesticide of carrying out research, the pollution-free food production base of transgenic pest-resistant, disease-resistant crop does not have and detects requirement etc. and all need very strong science and technology and funds support, apply from developing to as a kind of agricultural chemicals, need multi-disciplinary cooperations such as chemistry, agronomy, medical science and environment, go through 10 surplus year.According to the national conditions of China, present stage is the use of impossible total ban organophosphorous pesticide also.Therefore, develop a kind of can the degraded and do the residual of the interior organophosphorous pesticide of object, the biologic product that can promote plant growth again is current and presses for from now on.

Rare earth application is China's initiative, and has become one of major measure of agricultural production and income.At present relevant RE microbial fertilizer (as " Chang Le ") and relevant patent are as 96109036.7, manufacture method of solvay-type RE microbial fertilizer and products thereof, 96117083.2, serial compound rare-earth trace element fertilizer and production method thereof, 98103155.2, a kind of as fertile medicine the solubility compound powder and the main purpose of its developments such as other biologic product and biologic product be to promote plant growth and improve quality, and existing promotion plant growth has the product for agriculture of decreasing organic phosphorous agricultural chemicals to have not yet to see patent report.To the physiological and biochemical research of rare earth and tealeaves isoreactivity composition the time, find, natural tea polysaccharide is combined with rare earth element, this natural malt sugar soil compound and tea polysaccharide are that part and rare earth element ligand complex are prepared the polysaccharide RE composite, and the phosphide key is all had very strong splitting action.Organophosphorous pesticide contains the phosphide key mostly, and therefore, the polysaccharide RE composite has degradation to the dense medicine of organic phosphor, but has not yet to see the patent of invention report.

Summary of the invention

The objective of the invention is to overcome the deficiency of prior art, make full use of the discarded object of existing tealeaves industrial enterprise, a kind of preparation method of biologic product of decreasing organic phosphorous pesticide residue and promoting growth is provided.

The preparation method 1: do not add the preparation method that rare earth directly utilizes tealeaves industrial enterprise discarded object: can directly utilize the tea polysaccharide waste liquid that contains behind Tea Processing Tea Polyphenols and the caffeine to concentrate the back and regulate pH value to 4～7, filter, and further concentrate liquid biologic product, dry solid-state biologic product.

The preparation method 2: add the preparation method that rare earth utilizes tealeaves industrial enterprise discarded object: if when not containing rare earth element in this discarded object, can utilize and contain the tea polysaccharide waste liquid behind Tea Processing Tea Polyphenols and the caffeine, after concentrating, adding is 1～3 rare earth or directly uses agricultural nitric acid rare earth with the pH of dilute nitric acid dissolution in advance, the percentage that rare earth adding quantity accounts for tea polysaccharide discarded object weight is controlled at 0.00%～0.5%, react 5～24h down at 10～35 ℃, transfer reacting liquid pH value to 4～7 with acid or alkali, filtering and concentrating gets liquid biologic product, or dry solid-state biologic product.

The preparation method 3: solid-state tea polysaccharide discarded object dissolving back adding is 1～3 rare earth or directly uses agricultural nitric acid rare earth with the pH of dilute nitric acid dissolution in advance, the percentage that rare earth adding quantity accounts for tea polysaccharide discarded object weight is controlled at 0.00%～0.5%, react 5～24h down at 10～35 ℃, transfer reacting liquid pH value to 4～7, filter, concentrate liquid biologic product, or dry solid-state biologic product.

With the biologic product of said method processing, application can make organophosphorous pesticide reach more than 80% at degradation rate after a couple of days on the crop that has sprayed organophosphorous pesticide, and the crop yield rate is 3～20%, and the crop yield probability is about 85%.In addition, this biologic product is a kind of natural medicine or contains more a spot of rare earth, thereby not only greatly reduced the usage amount of rare earth, improved the safety of rare earth application, and made the tea resources of China's abundant obtain abundant higher value application.

The present invention also has production process not have distinguishing features such as waste gas, contaminated wastewater environment, raw material are easy to get, technology is simple, investment is very little, therefore the technological transformation needs that not only are fit to processing enterprise of existing Tea Polyphenols factory, improve its benefit, and can produce and be applicable in rural area medium-sized and small enterprises, be a kind of new technology that current pollution-free food production presses for.

Embodiment

The tea polysaccharide discarded object with warm water dissolving (when producing in processing enterprise of Tea Polyphenols factory, can be directly with the liquid that contains tea polysaccharide, needn't dissolve), solvability is controlled at 2%～35%, slowly adding is 1～3 rare earth or directly uses nitric acid rare earth that rare earth adding quantity is controlled at 0.00%～0.5% (accounting for the percentage of tea polysaccharide discarded object weight) in advance with the pH of dilute nitric acid dissolution then, and pH is controlled at 2～6, and after remaining on 25 ℃～35 ℃ reaction 24h, with acid, alkali fine setting reacting liquid pH value to 4～7.Filter the back concentrate liquid biologic product, or dry solid-state biologic product.No matter the final products of preparation are liquid state or solid-state shape, all should include the active ingredient carbohydrate in their every mu of usage amount is 30g～150g (by dropping into raw material), and rare earth is 0.0001g～20g (by dropping into raw material).Rare earth used among the above-mentioned preparation method can directly be planted element (the little fertile factory in Shangqiu produces) or other agricultural nitric acid rare earth with " Chang Le " board benefit, and this biologic product field using method is with conventional biologic product using method.

Embodiment 1

Be dissolved in the 1000ml water with tea polysaccharide 50g prepared behind Tea Processing Tea Polyphenols and the caffeine, slowly add 0.2g " Chang Le " board benefit and plant element, after remaining on 25 ℃ of reaction 24h, with carbonic hydroammonium fine setting reacting liquid pH value to 6.5, filter the back concentrate liquid biologic product, or in the biologic product of making an uproar solid-stately.No matter be liquid biologic product, or solid-state biologic product, tea polysaccharide content 500g in every mu of usage amount (, as follows), content of rare earth 2g (, as follows) by dropping into raw material by dropping into raw material.

Embodiment 2

With after containing the tea polysaccharide waste liquid and concentrate behind Tea Processing Tea Polyphenols and the caffeine, leave standstill 24h after, the biologic product that filters liquidly, and then dry solid-state biologic product.Contain tea polysaccharide solid content 500g in every mu of usage amount.

Claims

1. the preparation method of a biological preparation decreasing organic phosphorous pesticide residue and promoting growth is characterized in that directly utilizing the tea polysaccharide waste liquid that contains behind Tea Processing Tea Polyphenols and the caffeine to concentrate the back and regulate pH value to 4～7, concentrated liquid biologic product.

2. the preparation method of a biological preparation decreasing organic phosphorous pesticide residue and promoting growth, after it is characterized in that directly utilizing containing the tea polysaccharide waste liquid and concentrate behind Tea Processing Tea Polyphenols and the caffeine, adding is 1～3 rare earth or directly uses agricultural nitric acid rare earth with the pH of dilute nitric acid dissolution in advance, the percentage that rare earth adding quantity accounts for the tea polysaccharide dry weight is controlled at 0.00%～0.5%, react 5～24h down at 10～35 ℃, transfer reacting liquid pH value to 4～7 with acid or alkali, filtering and concentrating gets liquid biologic product.

3. the preparation method of a biological preparation decreasing organic phosphorous pesticide residue and promoting growth, it is characterized in that solid-state tea polysaccharide dissolving back adding is 1～3 rare earth or directly uses agricultural nitric acid rare earth with the pH of dilute nitric acid dissolution in advance, the percentage that rare earth adding quantity accounts for tea polysaccharide weight is controlled at 0.00～0.5%, react 5～24h down at 10～35 ℃, transfer reacting liquid pH value to 4～7, filter, concentrate liquid biologic product.