CN101117621A - Novel method for white fungus cultivation - Google Patents
Novel method for white fungus cultivation Download PDFInfo
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- CN101117621A CN101117621A CNA2007100759619A CN200710075961A CN101117621A CN 101117621 A CN101117621 A CN 101117621A CN A2007100759619 A CNA2007100759619 A CN A2007100759619A CN 200710075961 A CN200710075961 A CN 200710075961A CN 101117621 A CN101117621 A CN 101117621A
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Abstract
The present invention relates to a planting method of edible fungus, and provides a new method for white fungus planting. The method includes steps as follows: the raw material of a substrate is mixed, bagged and sterilized, the planting seed of white fungus is inoculated and planted, and the grew fungus is picked, the fungus age of the planting seed of the white fungus is 6 to 10 days; the planting steps are described as follows: after being inoculated, the planting seed is planted for 16 days when the planting temperature is 23 to 25 DEG C, adhesive plaster at the opening of an aperture is torn, the aperture is not expanded, after 5 to 6 days, the side of the opening of the aperture of a fungus bag, namely the side of the fungus bag is pulled a crack at the bag film by a blade. The application provides that the most ideal fungus age of the planting seed of white fungus is 6to 10 days, compared with the decided fungus age, the ideal fungus age is shortened 5 to 9 days, thereby quickening the fungus seed supplying time, increasing production amount of fungus seed, efficiently solving the supply and demand contradictory of fungus seed at the midseason for white fungus producing, and meeting the demands for big amount of production. In addition, the new technology that the fungus bag increases oxygen and produces white fungus provided by the application has the advantages of convenient operation, quickened white fungus producing, few pedicel heads of white fungus, and higher quality than the original products.
Description
Technical field
The present invention relates to the cultivating method of edible fungus, relate in particular to the selection of white fungus bacterial classification cell age in the cultivating white fungus process and go out the ear novel method.
Background technology
White fungus has another name called Tremella, tremella, and formal name used at school Tremella fuciformis Beyk, English name White Fungus is China tradition food medicine dual-purpose fungi.Behind the white fungus substituting stuff cultivation, with short production cycle, from being inoculated into harvest only 35-40 days, and be fit to the white fungus bacterial classification of substituting stuff cultivation, general 15 days of validity period, the time limit is shorter.Therefore the white fungus seeding technique is the most difficult in a many bacterial classifications kind.Because warm type bacterial classification in the Tremella, under natural climate condition, cultivation season is concentrated spring and autumn, and the peak period that bacterial classification uses is more concentrated.By the cultivar cell age of eighties regulation 17～20 days, 15 days have been changed in recent years.But in the peak period that white fungus is produced, the bacterial classification supply often occurs and can not satisfy mushroom farming requirement, form disparities between supply and demand.In spring end, for kind of a time lag, mushroom farming cultivation season is incured loss through delay, and when being everlasting original hase differone entity, temperature surpasses 25 ℃, inoculation cave vomiting of blackish fluid occurs, causes mashed ear; And in autumn end, Chang Yin is not in good time for planting, and when temperature was lower than 18 ℃, cave, inoculation back mouth was secreted white mucus, also causes the original hase poorly differentiated, and sporophore growth is affected.How to select the suitableeest cell age of cultivating white fungus kind to become current urgent problem.
In addition, how producing high-quality white fungus also is the problem that people pay close attention to.
Summary of the invention
The object of the present invention is to provide a kind of new method for cultivating white fungus.
Technical scheme of the present invention is in order to achieve the above object: a kind of new method for cultivating white fungus, comprise the steps: culture medium raw material stirring, pack, sterilization, the inoculation of white fungus bacteria cultivation kind, cultivation, fruiting and gather, the cell age of described cultivating white fungus kind is 6～10 days, described culturing step is that cultivar is after inoculation, when being 23～25 ℃, bacterium bag culture temperature cultivated 16 days, cultivated 18 days when temperature is low, tear cave chewing-gum cloth off and do not expand the cave, again through after 5～6 days, side at bacterium bag cave mouth, be bacterium bag next door, with blade standardized crack on the bag film.
The cell age of described cultivating white fungus kind is 6 days.
Described crack is the logical oxygen crack of 3～4 centimetres of length.
It is 6～10 days that the application provides the suitableeest cell age of cultivating white fungus kind, than the 15 days fixed scopes of rectifying, shortened 5～9 days, thereby accelerated to provide the bacterial classification supply time and increase the bacterial classification production quantity, solve white fungus effectively and produce busy season bacterial classification disparities between supply and demand, satisfy big production demand.The bacterium bag oxygenation that provides of the application goes out the ear novel process and has following advantage in addition: 1) easy to operate, can save 4 workdays for per 1000 bags; 2) accelerated ear 1～2 day; 3) white fungus base of a fruit head is few, and quality is higher than existing product.
Embodiment
Embodiment 1
One, material: white fungus bacterial strain TR29
Culture medium prescription: cotton seed hulls 84%, wheat bran 15%, terra alba 1%, material and water
1：1.2，PH5.8～6.2。
Two, step
1. culture bag prepares: 12 * 55cm low pressure polyethylene bag of packing into after according to culture medium prescription above-mentioned supplementary material being stirred after normal-pressure sterilization reaches 100 ℃, kept 24 hours.
2. inoculation: the cell age of choosing 4～15 days 6 gradients is seeded in the culture bag, and each cell age respectively connects 1000 bags.
3. cultivate: after inoculation, bacterium bag culture temperature is 23～25 ℃, cultivates 16 days, tear cave chewing-gum cloth off and do not expand the cave, and again through after 6 days, in the side of bacterium bag cave mouth, i.e. bacterium bag next door, usefulness blade logical oxygen crack of standardized long 3-4 centimetre on the bag film.
Three, result
The suitableeest cell age of cultivating white fungus kind can be grasped between 6～10 days, saw Table 1
The different cell ages of table 1 cultivating white fungus kind are used for the production test contrast
| Cell age (my god) | The mycelial growth situation | Go out the ear fate | Bright ear output (kg) | Contrast ± % |
| 15 | Incense ashes mycelia look greyish black, stretches at the bottom of the culture material bottle, and the existing rice krispies shape of the white hair ball in surface has a small amount of ear bud to occur, and accompanies the yellow red globule; It is prosperous that mycelia climbs wall, brown secretory product | 18 | 60.2 | Control group |
| 12 | Incense ashes mycelia look greyish black, stretches in the material 1/3; Surface white peas or beans grain is big, densely covered, accompanies the yellow red globule; Climb wall mycelia browning, secretory product is many | 19 | 61.6 | +0.23 |
| 10 | The incense ashes white mycelium, rare slightly, stretch into 40mm in the material, close of the white hair ball in surface, the full look of rice grain is pure white, and it is strong that mycelia climbs wall power | 19 | 63.3 | +0.51 |
| 8 | The incense ashes mycelia is sparse in vain, stretches into 30mm in the material, and the white hair ball in surface gathers, and grain is medium and small, and look dense is white; Mycelia climbs wall a bit | 20 | 64.8 | +0.76 |
| 6 | The incense ashes mycelia is greyish white, and is rarer, stretches into 18mm in the material; The white hair ball in surface is obvious, and the mung bean grain is big, and look dense is white | 20 | 66 | +0.96 |
| 4 | The incense ashes mycelia is greyish white, and is rarer, stretches into 14mm in the material; The white hair ball in surface is fuzzy, and only small rice grain is big, and look greyish white, and it is sparse to distribute | 22 | 60.5 | +0.03 |
Cell age is 6～10 days as seen from Table 1, and the mycelia vitality is strong, decomposes to absorb the nutrient abundance, and the time of nourishing and growing prolongs relatively, and reproductive growth is also suitable mutually to be extended, so go out ear slow 2 days than 15 days cell ages.Because accumulation nutrient abundance, the reproductive growth ability is strong, and also than the growth respectively 0.51%, 0.76%, 0.96% of 15 days cell ages of control group, wherein performance better is 6 days cell ages to output.And the bacterial classification of 4 days cell ages went out ear time ratio control group slow 4 days, and than the suitableeest cell age slow 2 days, output and control group were approaching, with 6～10 days cell ages differ 0.93%, 0.73%, 0.48%.
The suitableeest cell age of cultivating white fungus kind is 6～10 days, than the 15 days fixed scopes of rectifying, has shortened 5～9 days, thereby accelerates to provide the bacterial classification supply time and increase the bacterial classification production quantity, solves white fungus effectively and produces busy season bacterial classification disparities between supply and demand, satisfies big production demand.
The bacterium bag oxygenation that provides of the application goes out the ear novel process in addition, promptly in the side of bacterium bag cave mouth with blade standardized same crack on the bag film, have following advantage: 1) easy to operate, can save 4 workdays for per 1000 bags; 2) accelerated ear 1～2 day; 3) white fungus base of a fruit head is few, and quality is higher than existing product.
Claims (3)
1. new method for cultivating white fungus, comprise the steps: culture medium raw material stirring, pack, sterilization, the inoculation of white fungus bacteria cultivation kind, cultivation, fruiting and gather, the cell age that it is characterized in that described cultivating white fungus kind is 6～10 days, described culturing step is that cultivar is after inoculation, when being 23～25 ℃, bacterium bag culture temperature cultivated 16 days, cultivated 18 days when temperature is low, tear cave chewing-gum cloth off and do not expand the cave, again through after 5～6 days, side at bacterium bag cave mouth, be bacterium bag next door, with blade standardized crack on the bag film.
2. a kind of new method for cultivating white fungus according to claim 1, the cell age that it is characterized in that described cultivating white fungus kind is 6 days.
3. a kind of new method for cultivating white fungus according to claim 1 is characterized in that the logical oxygen crack of described crack for 3～4 centimetres of length.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100759619A CN101117621B (en) | 2007-07-10 | 2007-07-10 | Novel method for white fungus cultivation |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2007100759619A CN101117621B (en) | 2007-07-10 | 2007-07-10 | Novel method for white fungus cultivation |
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| CN101117621A true CN101117621A (en) | 2008-02-06 |
| CN101117621B CN101117621B (en) | 2010-06-09 |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101755616A (en) * | 2010-03-15 | 2010-06-30 | 林文华 | Method for manufacturing tremella |
| CN102093959A (en) * | 2010-12-13 | 2011-06-15 | 华南农业大学 | Production method of recombinant multifunctional cellulase |
| CN102972210A (en) * | 2012-12-12 | 2013-03-20 | 张德品 | New tremella cultivation method |
| CN103125267A (en) * | 2011-12-03 | 2013-06-05 | 四川省农业科学院土壤肥料研究所 | Cut-log tremella seed dressings and manufacturing method thereof |
| CN103125266A (en) * | 2011-12-03 | 2013-06-05 | 通江县银耳科学技术研究所 | New tremella short cut-log clinker layer frame cultivation method |
| CN103283492A (en) * | 2013-06-28 | 2013-09-11 | 福建省邵武市绿农食用菌有限公司 | Method for cultivating tremella fuciformis |
| CN103467176A (en) * | 2013-08-15 | 2013-12-25 | 安徽中祝农业发展有限公司 | Tremella cultivation medium and preparation method thereof |
| CN103493680A (en) * | 2013-09-23 | 2014-01-08 | 重庆市中药研究院 | Tremella liquid cultivar culture method and special culture medium for cultivar |
| CN103535184A (en) * | 2013-08-24 | 2014-01-29 | 江剑峰 | White fungus cultivation method |
| CN104737796A (en) * | 2015-03-16 | 2015-07-01 | 邬金梅 | Tremella cultivation method |
| CN105145120A (en) * | 2015-09-29 | 2015-12-16 | 古田县聚珍堂食品有限公司 | Tremella cultivation method |
| CN106105773A (en) * | 2016-06-24 | 2016-11-16 | 四川省大真科技有限责任公司 | The producing method for seed of edible mushroom cultivated species and prepared cultigen and cultivating method for edible fungi |
| CN107955802A (en) * | 2017-12-05 | 2018-04-24 | 福建农林大学 | A kind of method of thickness wall spore Pu Keniya bacteria solid fermentations production spore |
-
2007
- 2007-07-10 CN CN2007100759619A patent/CN101117621B/en not_active Expired - Fee Related
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101755616A (en) * | 2010-03-15 | 2010-06-30 | 林文华 | Method for manufacturing tremella |
| CN102093959A (en) * | 2010-12-13 | 2011-06-15 | 华南农业大学 | Production method of recombinant multifunctional cellulase |
| CN102093959B (en) * | 2010-12-13 | 2012-11-07 | 华南农业大学 | Production method of recombinant multifunctional cellulase |
| CN103125267B (en) * | 2011-12-03 | 2014-07-30 | 四川省农业科学院土壤肥料研究所 | Cut-log tremella seed dressings and manufacturing method thereof |
| CN103125267A (en) * | 2011-12-03 | 2013-06-05 | 四川省农业科学院土壤肥料研究所 | Cut-log tremella seed dressings and manufacturing method thereof |
| CN103125266A (en) * | 2011-12-03 | 2013-06-05 | 通江县银耳科学技术研究所 | New tremella short cut-log clinker layer frame cultivation method |
| CN102972210A (en) * | 2012-12-12 | 2013-03-20 | 张德品 | New tremella cultivation method |
| CN103283492B (en) * | 2013-06-28 | 2015-10-28 | 福建省邵武市绿农食用菌有限公司 | A kind of method of cultivating white fungus |
| CN103283492A (en) * | 2013-06-28 | 2013-09-11 | 福建省邵武市绿农食用菌有限公司 | Method for cultivating tremella fuciformis |
| CN103467176A (en) * | 2013-08-15 | 2013-12-25 | 安徽中祝农业发展有限公司 | Tremella cultivation medium and preparation method thereof |
| CN103535184A (en) * | 2013-08-24 | 2014-01-29 | 江剑峰 | White fungus cultivation method |
| CN103493680A (en) * | 2013-09-23 | 2014-01-08 | 重庆市中药研究院 | Tremella liquid cultivar culture method and special culture medium for cultivar |
| CN104737796A (en) * | 2015-03-16 | 2015-07-01 | 邬金梅 | Tremella cultivation method |
| CN105145120A (en) * | 2015-09-29 | 2015-12-16 | 古田县聚珍堂食品有限公司 | Tremella cultivation method |
| CN105145120B (en) * | 2015-09-29 | 2017-11-07 | 古田县聚珍堂食品有限公司 | A kind of cultivating white fungus method |
| CN106105773A (en) * | 2016-06-24 | 2016-11-16 | 四川省大真科技有限责任公司 | The producing method for seed of edible mushroom cultivated species and prepared cultigen and cultivating method for edible fungi |
| CN107955802A (en) * | 2017-12-05 | 2018-04-24 | 福建农林大学 | A kind of method of thickness wall spore Pu Keniya bacteria solid fermentations production spore |
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| Publication number | Publication date |
|---|---|
| CN101117621B (en) | 2010-06-09 |
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