CN101104645A - Method for extracting balloonflower polysaccharide assisted by ultrasound wave - Google Patents
Method for extracting balloonflower polysaccharide assisted by ultrasound wave Download PDFInfo
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- CN101104645A CN101104645A CNA2007100155977A CN200710015597A CN101104645A CN 101104645 A CN101104645 A CN 101104645A CN A2007100155977 A CNA2007100155977 A CN A2007100155977A CN 200710015597 A CN200710015597 A CN 200710015597A CN 101104645 A CN101104645 A CN 101104645A
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Abstract
The invention provides a technique for extracting balloonflower polysaccharide with the assistance of the ultrasonic, which is characterized in the following steps: (1) balloonflower powder is soaked in the solvent which is as 40 to 50 times milliliter as the quality grammage of the balloonflower powder, and whose PH regulated by the acid is between 5 to 6 and the ethanol concentration is between 40 percent to 60 percent within 35 to 45 min; (2) the solvent in which the balloonflower powder is soaked is put into a vessel, and the vessel is arranged on an ultrasonic equipment whose power is between 150 to 200 W and the temperature is between 60 to 75 DEG C for 35 to 40 min to obtain the extract; (3) the ph of the extract is regulated by alkali to neutral and is extracted and filtered. Filtrate is concentrated to dry under the temperature between 60 to 70 DEG C and atmospheric pressure between 0.1 to 0.7, and then is transferred by being dissolved in the water and is frozen to dry to obtain the balloonflower polysaccharide. The technique only uses the ethanol and little acid and alkali which are all not harmful to human body and are cheap and available. Compare with the traditional technique, the invention has the advantages that the technique and the production process are simple; the yield is high; the amount of the ethanol, the acid and the alkali are reduced greatly; the operation is easy, and the industrialization, the mechanization and the automation are easy to realize.
Description
Affiliated technical field
The invention provides a kind of processing method of using the ultrasonic-assisted extraction platycodon root polysaccharide, belong to technical field of agricultural product process.
Background technology
Balloonflower root is one of main traditional Chinese medicine of China, and it is fully developed as yet, the crust of balloonflower root particularly, cauline leaf etc.Contain protein 3.5g, fatty 1.2g, carbohydrate 18.2g, calcium 260mg, phosphorus 40mg, iron 13mg, carotene 2.2mg, vitamins C 10mg, VitB1 0.45mg, riboflavin 0.44mg, food fibre 3.2g in the bright balloonflower root of every 100g.Platycodon root polysaccharide is a main active ingredient in the balloonflower root, points out that as Sang B.Han platycodon root polysaccharide can selectivity activating B cell and scavenger cell in " Polysaccharide isolated from the radix of Platycodon grandiflorum selectively activates Bcells and macrophages but not T cells " literary composition that calendar year 2001 " International Immunopharmacology " delivers.
And traditional extraction process mainly is the solvent method, the leach extraction method that is adopted in " the Radix Platycodonis extract method " delivered in 2002 the 6th phases " Chinese medicinal materials " as Wu Biyuan, though technology is stablized feasible, efficient is low, length consuming time, the reagent consumption is big, and solvent is easily residual, and environment is had certain infringement.Though the method that acidolysis alkali is carried has certain effect, yet concentration is excessive, etching apparatus reduced the length of service of equipment, and concentration is low excessively, poor effect.And ultrasonic method is a kind of emerging extracting method, and its principle is that material is had mechanical stirring, heating and cavatition.Therefore, the material in the cell is dissolved in the solvent fast.Yet, for the research of ultrasonic-assisted extraction platycodon root polysaccharide, report is not arranged but at present.
Summary of the invention
The purpose of this invention is to provide a kind of can overcome above-mentioned defective, weak point consuming time, efficient height, simple to operate, production cost is low, to the processing method of the low utilization ultrasonic-assisted extraction platycodon root polysaccharide of equipment corrosion.Its technical scheme is:
A kind of processing method of using the ultrasonic-assisted extraction platycodon root polysaccharide is characterized in that adopting following steps: (1) with balloonflower powder with 40～50 times of milliliters of its quality gram number, with acid for adjusting pH be 5～6, alcohol concn is solution soaking 35～45min of 40%～60%; (2) immersion is had the solution of balloonflower powder contain in the container, it is that 150～200W, temperature are to extract 35～40min in 60～75 ℃ of ultrasonic equipments that container is placed on power, obtains extracting solution; (3) regulate extracting solution pH to neutral with alkali, suction filtration then, and filtrate is concentrated under 60～70 ℃, 0.1～0.7 normal atmosphere dried, water solution transfer again, freeze-drying gets platycodon root polysaccharide.
The processing method of described utilization ultrasonic-assisted extraction platycodon root polysaccharide comprises stem, root, the leaf of balloonflower root in the balloonflower powder.
The processing method of described utilization ultrasonic-assisted extraction platycodon root polysaccharide, regulating the used acid of pH in the step 1 is hydrochloric acid or sulfuric acid etc.
The processing method of described utilization ultrasonic-assisted extraction platycodon root polysaccharide, ethanolic soln is an aqueous ethanolic solution in the step 1, prepares with alcoholometer.
The processing method of described utilization ultrasonic-assisted extraction platycodon root polysaccharide, used container is flask or the similar device that can connect water of condensation circulation tube, good airproof performance in the step 2.
The processing method of described utilization ultrasonic-assisted extraction platycodon root polysaccharide, container will connect water of condensation in the step 2.
The processing method of described utilization ultrasonic-assisted extraction platycodon root polysaccharide, regulating the used alkali of extracting solution pH in the step 3 is sodium hydroxide.
The present invention compared with prior art, the technology that is adopted has only been used acid, the alkali of ethanol and minute quantity, these three kinds of materials are all almost harmless and cheap and easy to get to human body, compare with traditional technology, technology is simple, the yield height, and the ethanol consumption significantly reduces, the consumption of acid, alkali also reduces greatly, production process is simple, and is easy to operate, easily industrialization, mechanize, automatic production.
Embodiment
Embodiment 1:
Step 1: to be 10～30 balloonflower powder with 20g order number with 900mL, with hydrochloric acid regulate pH is 5, alcohol concn is 60% solution soaking 40min;
Step 2: have the solution of balloonflower powder to contain in the flask immersion, it is that 150W, temperature are in 75 ℃ of ultrasonic equipments that flask is placed on power, and ultrasonic extraction 35min obtains extracting solution;
Step 3: regulate extracting solution pH to neutral with sodium hydroxide, suction filtration then, and filtrate is concentrated under 65 ℃, 0.1 normal atmosphere dried, water solution transfer again, freeze-drying gets platycodon root polysaccharide.
Embodiment 2:
Step 1: to be 10～30 balloonflower powder with 20g order number with 1000mL, with hydrochloric acid regulate pH is 5, alcohol concn is 40% solution soaking 45min;
Step 2: have the solution of balloonflower powder to contain in the flask immersion, it is that 200W, temperature are in 60 ℃ of ultrasonic equipments that flask is placed on power, and ultrasonic extraction 40min obtains extracting solution;
Step 3: regulate extracting solution pH to neutral with sodium hydroxide, suction filtration then, and filtrate is concentrated under 70 ℃, 0.5 normal atmosphere dried, water solution transfer again, freeze-drying gets platycodon root polysaccharide.
After measured, contain platycodon root polysaccharide in the extract of acquisition; Calculate through colorimetric method for determining, the polysaccharide yield of two examples is respectively 35.4% and 36.7%.
Claims (7)
1. processing method of using the ultrasonic-assisted extraction platycodon root polysaccharide is characterized in that adopting following steps: (1) with balloonflower powder with 40～50 times of milliliters of its quality gram number, with acid for adjusting pH be 5～6, alcohol concn is solution soaking 35～45min of 40%～60%; (2) immersion is had the solution of balloonflower powder contain in the container, it is that 150～200W, temperature are to extract 35～40min in 60～75 ℃ of ultrasonic equipments that container is placed on power, obtains extracting solution; (3) regulate extracting solution pH to neutral with alkali, suction filtration then, and filtrate is concentrated under 60～70 ℃, 0.1～0.7 normal atmosphere dried, water solution transfer again, freeze-drying gets platycodon root polysaccharide.
2. the processing method of utilization ultrasonic-assisted extraction platycodon root polysaccharide as claimed in claim 1 is characterized in that: the stem, root, the leaf that comprise balloonflower root in the balloonflower powder.
3. the processing method of utilization ultrasonic-assisted extraction platycodon root polysaccharide as claimed in claim 1 is characterized in that: regulating the used acid of pH in the step 1 is hydrochloric acid or sulfuric acid etc.
4. the processing method of utilization ultrasonic-assisted extraction platycodon root polysaccharide as claimed in claim 1 is characterized in that: ethanolic soln is an aqueous ethanolic solution in the step 1, prepares with alcoholometer.
5. the processing method of utilization ultrasonic-assisted extraction platycodon root polysaccharide as claimed in claim 1 is characterized in that: used container is flask or the similar device that can connect water of condensation circulation tube, good airproof performance in the step 2.
6. the processing method of utilization ultrasonic-assisted extraction platycodon root polysaccharide as claimed in claim 1 is characterized in that: container will connect water of condensation in the step 2.
7. the processing method of utilization ultrasonic-assisted extraction platycodon root polysaccharide as claimed in claim 1 is characterized in that: regulating the used alkali of extracting solution pH in the step 3 is sodium hydroxide.
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| CNB2007100155977A CN100554283C (en) | 2007-06-22 | 2007-06-22 | The processing method of utilization ultrasonic-assisted extraction platycodon root polysaccharide |
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| CNB2007100155977A CN100554283C (en) | 2007-06-22 | 2007-06-22 | The processing method of utilization ultrasonic-assisted extraction platycodon root polysaccharide |
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| CN101104645A true CN101104645A (en) | 2008-01-16 |
| CN100554283C CN100554283C (en) | 2009-10-28 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101830999A (en) * | 2010-05-21 | 2010-09-15 | 王振宇 | Method for extracting platycodon root polysaccharide |
| CN101891836A (en) * | 2010-07-27 | 2010-11-24 | 乔德亮 | Method for extracting white mushroom lentinan assisted with ultrasonic wave |
| CN101445529B (en) * | 2008-12-19 | 2011-05-04 | 山东理工大学 | Process for simultaneously producing platycodon anthocyanin and polysaccharide single component |
| CN102423334A (en) * | 2011-09-30 | 2012-04-25 | 淮南联合大学 | Comprehensive extraction method for platycodin and platycodon grandiflorum polysaccharide |
| CN102919918A (en) * | 2012-10-19 | 2013-02-13 | 宽甸满族自治县光太药材有限公司 | Method for making clarified platycodon grandiflorum beverage and obtained clarified platycodon grandiflorum beverage |
| CN104292355A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Method for extraction of platycodon grandiflorum polysaccharide |
| CN104311692A (en) * | 2014-11-20 | 2015-01-28 | 四川旭华制药有限公司 | Method for extracting polysaccharide substance in rhizophora mucronata through ultrasonic waves |
| CN104844721A (en) * | 2015-04-20 | 2015-08-19 | 苏州经贸职业技术学院 | Extraction and separation method of Agrocybe aegirit polysaccharides |
| CN107936133A (en) * | 2018-01-16 | 2018-04-20 | 吉林大学 | A kind of redsepal evening primrose leaf polysaccharide and preparation method thereof |
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2007
- 2007-06-22 CN CNB2007100155977A patent/CN100554283C/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101445529B (en) * | 2008-12-19 | 2011-05-04 | 山东理工大学 | Process for simultaneously producing platycodon anthocyanin and polysaccharide single component |
| CN101830999A (en) * | 2010-05-21 | 2010-09-15 | 王振宇 | Method for extracting platycodon root polysaccharide |
| CN101891836A (en) * | 2010-07-27 | 2010-11-24 | 乔德亮 | Method for extracting white mushroom lentinan assisted with ultrasonic wave |
| CN101891836B (en) * | 2010-07-27 | 2012-05-02 | 乔德亮 | Method for extracting white mushroom lentinan assisted with ultrasonic wave |
| CN102423334A (en) * | 2011-09-30 | 2012-04-25 | 淮南联合大学 | Comprehensive extraction method for platycodin and platycodon grandiflorum polysaccharide |
| CN102423334B (en) * | 2011-09-30 | 2014-05-14 | 淮南联合大学 | Comprehensive extraction method for platycodin and platycodon grandiflorum polysaccharide |
| CN102919918B (en) * | 2012-10-19 | 2014-01-22 | 宽甸满族自治县光太药材有限公司 | Method for making clarified platycodon grandiflorum beverage and obtained clarified platycodon grandiflorum beverage |
| CN102919918A (en) * | 2012-10-19 | 2013-02-13 | 宽甸满族自治县光太药材有限公司 | Method for making clarified platycodon grandiflorum beverage and obtained clarified platycodon grandiflorum beverage |
| CN104292355A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Method for extraction of platycodon grandiflorum polysaccharide |
| CN104311692A (en) * | 2014-11-20 | 2015-01-28 | 四川旭华制药有限公司 | Method for extracting polysaccharide substance in rhizophora mucronata through ultrasonic waves |
| CN104844721A (en) * | 2015-04-20 | 2015-08-19 | 苏州经贸职业技术学院 | Extraction and separation method of Agrocybe aegirit polysaccharides |
| CN104844721B (en) * | 2015-04-20 | 2017-01-25 | 苏州经贸职业技术学院 | Extraction and separation method of Agrocybe aegirit polysaccharides |
| CN107936133A (en) * | 2018-01-16 | 2018-04-20 | 吉林大学 | A kind of redsepal evening primrose leaf polysaccharide and preparation method thereof |
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| CN100554283C (en) | 2009-10-28 |
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