CN101084736A

CN101084736A - Method for breeding restoring-type male-sterility cytoplasmic hybrid seeds of maize

Info

Publication number: CN101084736A
Application number: CN 200710123423
Authority: CN
Inventors: 堵纯信; 曹春景; 曹青; 毕蒙蒙
Original assignee: Individual
Current assignee: Individual
Priority date: 2007-06-25
Filing date: 2007-06-25
Publication date: 2007-12-12

Abstract

The invention relates to a production technology for preparing hybrid species of Zea mays called Zheng-958 by using male sterility three lines. The method comprises transferring mongrel seed-parent self-bred line Zheng 58 and Chang 7-2 respectively into male sterile line (named as Cms-Es Zheng 58) with Es sterility kytoplasm and restoring line (named as Cms-Es Chang 7-2 (Rf)).Sterile plant percentage of Sterile line can come up to 99.9%, Restoring plant percentage of restoring line can come up to 100%, and restoring degree came up to normal level. Zheng 958 is prepared with Cms-Es Zheng 58 as female parent and Cms-Es Chang 7-2 (Rf) as paternal seed without detasseling female parent, sterilized Zheng 958 hybrid species capable of flowering and dispersing pollen normally is obtained. the agronomic character, vital hardiness, and flexibility of the hybrid species is the same with conventional produced Zheng 958.The invention has the advantages of excused artificial emasculation, low cost, high seed quality, and high yield; and is suitable for large-area application.

Description

A kind of producing method for seed of corn recovery type male-sterility cytoplasmic hybrid kind

Technical field

The present invention relates to a kind of production of hybrid seeds new technology of corn hybrid seed, is the producing method for seed that utilizes male sterile " three are " preparation corn hybrid seed Zheng Dan 958, belongs to plant genetic and seed production field.

Background technology

The utilization of corn hybrid seed starts from the thirties in 20th century, is that genetics is applied to breeding, with the breeding application of result in producing and obtained huge economic results in society.China begins to carry out the corn variety improvement fifties and promotes from the last century, and from utilizing good farm variety, intervarietal cross kind, double cross hybrid to single cross hybrid, the cultivation and the utilization of corn hybrid seed are developed rapidly.

The conventional seed mode of production of the many employings of the production of present domestic corn hybrid seed, in the production of hybrid seeds process, before the tassel loose powder of female parent, all carry out the manual removal, not only expend a large amount of human resources, and often because of reason artificial or weather, maternal castration is difficult to clean, thorough, and maternal strain selfing and sisters hand over phenomenon to be difficult to avoid, and often causes seed purity to reduce, seed quality does not reach state specified standards, causes crossbreed (F ₁) underproduction.Now, guarantee that the most effective measure of corn hybrid seed seed purity utilizes the preparing maize sterility hybrid seed technology exactly.

Maize sterility hybrid, full name are recovery type corn male-sterility cytoplasmic hybrid kinds, and it is that (T type, S type, C type) and maintenance line thereof and male parent are recovered system and formed by maternal male-sterility, therefore, is three series mating crossbreed or three line hybrid seed again.The preparing maize sterility hybrid seed technology is widely used already in the U.S. and the former Soviet Union, and China because of various reasons, and is later to this Study on Technology, has influenced the application of the corn sterilization production of hybrid seeds in seed is produced.

Summary of the invention

The purpose of this invention is to provide a kind of sterilization producing method for seed of guaranteeing corn hybrid seed Zheng Dan 958 seed purities, solve the problem that exists in the conventional producing method for seed.Specifically be by the genetic breeding technology, maternal selfing Zheng 58 transformations are become male sterile line, the prosperous 7-2 transformation of male parent inbred line is become male sterile restoring line, realize that male sterile line, maintenance line, recovery are three series mating, utilize " three are " to carry out the seed production of Zheng Dan 958.

Research practice shows, is male-sterility system with the transformation of a certain ordinary maize inbred line, needs five, six generations, becomes the recovery system of male-sterility system then to need eight, nine generations just can finish at least a certain inbred line transformation.Be used for producing, also need therefore to require a great deal of time and painstaking effort through hybridization, evaluation, screening, breeding supervisor.The inventor has finished a kind of producing method for seed of maize sterility hybrid through concentrating on studies for many years.Wherein Yan Zhi whole procedure comprises male sterile line, the seed selection that recovers system and the preparation of corn hybrid seed Zheng Dan 958.Design of the present invention is: Zheng Dan 958 is ordinary maize crossbreed, maternal inbred line is Zheng 58, male parent is prosperous 7-2, it is fixed that this crossbreed was successively examined (recognizing) by provinces and regions such as national and Henan, Hebei, Shandong from 2000, add up popularizing area by 2006 and reached 1.9 hundred million mu, becoming the kind of national since the establishment of the nation cultivated area maximum, also is simultaneously the significant kind that the 6th corn variety of China updates.Therefore, this crossbreed also becomes the corn variety of annual production of hybrid seeds amount maximum, and the artificial emasculation in ordinary maize hybrid seeding process is time-consuming, take a lot of work, cost is high and become increasingly conspicuous because of castrating the untimely low problem of purity of hybrid that causes.The present invention promptly provides a kind of producing method for seed of sterilization Zheng Dan 958 corn hybrid seeds, to overcome the defective of prior art.

Particularly, method of the present invention may further comprise the steps:

(1) breeds male sterile line Zheng Cms-Es 58 of the Es type kytoplasm of sterile degree height, stable fertility

Combine 3 suppliers with Cms-Es, the maternal inbred Zheng 58 of Zheng Dan 958 is the new basis of samsara, backcross by continuous 11 generations, obtain sterile plant rate and reach Zheng's 58 male sterile lines of 99.9% as sterile cytoplasm.

(2) breed recovery strain rate and reach 100% the prosperous 7-2 of male sterile restoring line Cms-Es (Rf)

With Cms-Es comprehensive 3 and single-gene (Rf ₄Rf ₄) to recover be the F of new 40 hybridization ₁As the basic material of transformation, the former provides the sterile cytoplasm background, and the latter provides the recovery gene pool, at F ₁Middle some strains of selecting to recover are fully done maternal, be carried out to pollination with the prosperous 7-2 of male parent inbred line respectively, and with prosperous 7-2 as recurrent parent, backcross with the full recovery strain in the backcross progeny, carried out for 6 generations continuously, its economical character, regularity and prosperous 7-2 reach consistent after, by twice selfing and combining ability test, select the complete restitution of tool, good recovery that coordinate force is high is the prosperous 7-2 of Cms-Es (Rf).

(3) sterilization Zheng Dan 958 maize hybrid seed productions

With male-sterility is that Zheng Cms-Es 58 makes female parent, and recovery is the seed farm that the prosperous 7-2 of Cms-Es (Rf) makes male parent, and female parent is not castrated, and obtains normally to bloom solid maize sterility hybrid Zheng Dan 958 seeds of loose powder.

The present invention utilizes " three are " to carry out the seed production of Zheng Dan 958, removed the artificial emasculation operation of the maternal row of seed farm fully from, behind the crossbreed plantation land for growing field crops that is produced, can recover the loose powder ability fully, its output, economical character, resistance are identical with the Zheng Dan 958 that conventional method is produced, and some proterties also obtain improving.Adopt this technology to produce Zheng Dan 958 seeds, not only saved a large amount of throwing workers, and can guarantee the genetic purity of crossbreed, give full play to the yield potential of this kind.

Description of drawings

Fig. 1 is Es type male sterile line Zheng 58 transformation schematic diagrames.

Fig. 2 is that the recovery of Es type is prosperous 7-2 (Rf) transformation schematic diagram.

Fig. 3 is the seed production procedure schematic diagram that utilizes male sterile " three are " preparation Zheng Dan 958.

Embodiment

Following examples are in order to explanation the present invention.

Embodiment

The producing method for seed of sterilization Zheng Dan 958 corn hybrid seeds that present embodiment provides comprises the steps:

(1) Zheng Cms-Es 58 transformation

Referring to Fig. 1, the summer in 2000 was combined 3 suppliers as sterile cytoplasm with Cms-Es, be male parent with the maternal inbred Zheng 58 of Zheng Dan 958, formed comprehensive 3 * N Zheng 58 of Cms-Es, and the winter is sowed its F in Hainan then ₁Seed, taking out male loose powder phase, to select the male-sterility strain to do maternal, backcrosses the F that obtains with Zheng 58 as male parent ₁Seed was sowed in spring in calendar year 2001, selected sterile strain to backcross with Zheng 58 again, and continuous backcross is 9 times like this, obtains sterile plant rate and reaches Zheng Cms-Es 58 of 99.9%.

(2) recovery of Es type is the transformation of prosperous 7-2

Referring to Fig. 2, winter calendar year 2001 selects for use with Cms-Es comprehensive 3 and single-gene (Rf ₄Rf ₄) to recover be new 40 hybridization, kind of its F that sows in spring in 2002 ₁Seed is as the basic material of transformation, at F ₁Middle some strains of selecting to recover are fully done maternal, be carried out to pollination with the prosperous 7-2 of male parent inbred line respectively, and with prosperous 7-2 as recurrent parent, backcross with the full recovery strain in the backcross progeny, carried out for 6 generations continuously, its economical character, regularity and prosperous 7-2 reach consistent after, by twice selfing and combining ability test, select the complete restitution of tool, good recovery that coordinate force is high is the prosperous 7-2 of Cms-Es (Rf).

(3) sterilization Zheng Dan 958 hybrid maize seed production methods

Referring to Fig. 3, with Zheng Cms-Es 58 is female parent, Zheng N 58 is male parent, put under the above distance isolation condition of 500M, maternal and male parent is planted at interval by 5: 1 (or 6: 1) row ratio, and planting density 5500～6000 strain/mus are after the male parent loose powder finishes, all surgery, the seed of maternal row results is as the female parent of seed farm;

Recovery be the prosperous 7-2 of Cms-Es (Rf) at the above isolation implant of 500m, planting density 5500～6000 strain/mus, the seed that is produced is as the male parent of seed farm;

With male sterile line Zheng Cms-Es 58 is female parent, and recovery to be the prosperous 7-2 of Cms-Es (Rf) be male parent by 5: 1～or 6: 1 capable ratio, is carried out the production of hybrid seeds under the isolation condition more than 300 meters; Male parent row loose powder is all surgeried after finishing, and the seed of maternal row results is Zheng Dan 958 crossbreed of land for growing field crops production usefulness.

Claims

1, a kind of producing method for seed of corn recovery type male-sterility cytoplasmic hybrid kind, it is characterized in that: comprise that a C type Es subspecies male-sterility system does maternal, a C type recovers system and makes male parent, the row of taking to suit in secure isolation zone compares, suitable planting density, female parent such as does not castrate at supplementary measures, and step is as follows:

Combine 3 suppliers with Cms-Es, the maternal inbred Zheng 58 of Zheng Dan 958 is the new basis of samsara, backcross by continuous 11 generations, obtain sterile plant rate and reach Zheng's 58 male sterile lines of 99.9% as sterile cytoplasm;

(3) sterilization Zheng Dan 958 maize hybrid seed productions

2, by the described producing method for seed of claim 1, it is characterized in that: with male-sterility is that Zheng Cms-Es 58 makes female parent, and recovery is that the prosperous 7-2 of Cms-Es (Rf) makes male parent, seed farm mother, male parent row ratio are 5: 1 (or 6: 1), promptly broadcast 5 row (or 6 row) female parent, broadcast 1 row male parent, alternate plantation.

3, by the described producing method for seed of claim 1, it is characterized in that: with male-sterility is that Zheng Cms-Es 58 makes female parent, and recovery is that the prosperous 7-2 of Cms-Es (Rf) makes male parent, and the planting density of the field production of hybrid seeds is 5500～6000 strain/mus.

4, by the described producing method for seed of claim 1, it is characterized in that: with male sterile line Zheng Cms-Es 58 is female parent, and recovery is that the prosperous 7-2 of Cms-Es (Rf) is a male parent, carries out the production of hybrid seeds under the isolation condition more than 300 meters; Male parent row loose powder is all surgeried after finishing, and the seed of maternal row results is Zheng Dan 958.