CN100426949C - High-yield twin-corn plantation and its cenospecies selective breeding method - Google Patents
High-yield twin-corn plantation and its cenospecies selective breeding method Download PDFInfo
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- CN100426949C CN100426949C CNB2006100128123A CN200610012812A CN100426949C CN 100426949 C CN100426949 C CN 100426949C CN B2006100128123 A CNB2006100128123 A CN B2006100128123A CN 200610012812 A CN200610012812 A CN 200610012812A CN 100426949 C CN100426949 C CN 100426949C
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Abstract
The present invention discloses a method for planting high-yield twin corn, and breeding and preparing hybrids, which is characterized in that sterile hybrids and fertile hybrids are planted in fields in an interplanting mode or a mixed seeding mode. The sterile hybrids are bred by the procedures of sterile gene transformation breeding, backcross, continuous backcross, coordination of homotype sterile hybrids, and combining ability measurement. Compared with the original planted conventional corn single cross hybrids, twin corn hybrids planted by using the method have the yield increased by 14 to 22.88%. Only male sterile lines need to be bred, and restoring lines do not need to be bred. The present invention has the advantages of corn breeding procedure simplification, time saving and labor saving.
Description
Technical field
The present invention relates to the seed selection compound method of a kind of high yield mandarin duck maize planting and crossbreed, be specifically related to seed selection and the mandarin duck maize planting method of a kind of sterile corn crossbreed, belong to heterosis, hybrid vigor, male sterile and xenia effect and utilize research field.
Background technology
Corn is to utilize the most obvious, the maximum high-yield crop of yield potential of heterosis, hybrid vigor acquisition economic benefit, and what establishing in large scale was gone up in production for a long time is same single cross hybrid mostly, though utilized F
1The heterosis, hybrid vigor of plant is because the commodity food of results is F
2Seed, thereby certain inbreeding depression phenomenon (Duan Minxiao etc., calendar year 2001) is arranged.
Sterility cross-bred kind is to study the crossbreed of more a kind of newtype in recent years, has been report but yet there are no so far for the seed selection of sterility cross-bred kind.Application number a kind of male sterile maize hybrid seed production method that has been patent disclosures such as 200510012523.9 and 200510012669.3, but its so-called male sterile maize hybrid can be educated, though improved the output in hybrid maize seed production field, produced and do not avoid the inbreeding depression phenomenon when going up establishing in large scale yet; And this method also needs to cultivate and recover system, so the procedure of breeding is comparatively loaded down with trivial details except cultivating male sterile line and maintenance line.
Summary of the invention
The objective of the invention is to avoid the defective of said method, and provide that a kind of program is simple, the corn planting of corn hybrid seed production field increase yield significantly and the seed selection compound method of crossbreed, promptly utilize hybrid vigour, cytoplasmic male sterility to select sterility cross-bred kind, sterility cross-bred kind with can educate crossbreed and carry out that field cover is broadcast or the mixed seeding plantation, utilize the xenia effect to significantly improve corn hybrid seed output.
The technical problem to be solved in the present invention is achieved by the following technical solution: this high yield mandarin duck maize planting method is characterized in that a kind of sterile corn crossbreed and a kind of corn hybrid seed of educating are carried out that field cover is broadcast or the mixed seeding plantation.
The breeding hybridized compound method of described high yield mandarin duck corn, the seed selection of sterility cross-bred kind may further comprise the steps:
The transformation of A, sterile gene: the maternal selfing with the ordinary maize single cross hybrid is a male parent, is that female parent is hybridized with a kind of sterile selfing, obtains first-filial generation F
1Sterile seed;
B, backcross: plant first-filial generation F in the maternal inbred line of former ordinary maize single cross hybrid and the steps A
1Seed, tasseling stage is selected male sterile plant, does male parent with the maternal inbred line of former ordinary maize single cross hybrid and backcrosses, and obtains the sterile BC that a plurality of sisters are
1Seed notices that sisters system will gather in the crops respectively;
C, continuous backcross: plant sterile BC among the maternal inbred line of former ordinary maize single cross hybrid and the step B
1Seed, sisters system is plantation respectively, and tasseling stage is selected the higher sisters system of male sterile degree, does male parent with the maternal inbred line of former ordinary maize single cross hybrid and carries out secondary and backcross, so continuous backcross is 5 times, obtains a plurality of proterties sterile sisters comparatively identical with backcross parent to be;
The assembly of D, the sterility cross-bred kind of homotype: obtaining a plurality of sisters among the plantation step C is the backcross progeny seed, each sisters is plantation two row, the sterile strain of wherein delegation in each sisters system of tasseling stage continues to do male parent with the maternal inbred line of former ordinary maize single cross hybrid and backcrosses, directive breeding the offspring reserve seed for planting, the sterile strain of another row then with the male parent hybridization between selfed lines of former ordinary maize single cross hybrid, the sterility cross-bred kind of homotype that a plurality of male sterile hybrid combinations that obtain are former ordinary maize;
E, the coordinate force test of sterility cross-bred kind: the sterility cross-bred kind of homotype of a plurality of former ordinary maizes that seed selection becomes among the plantation step D, and plant a kind of crossbreed that educates simultaneously, each sterility cross-bred kind plantation 2 row, repeat 2 times, and numbering, the loose powder phase pollinates for sterility cross-bred kind with the pollen that can educate crossbreed fully blooming, select be the sterility cross-bred kind of using in the production of mandarin duck corn hybrid seed than the sterility cross-bred kind of former ordinary maize crossbreed obvious effect of increasing production the present age, the sterility cross-bred kind of obvious effect of increasing production and the educated crossbreed of plantation are a pair of mandarin duck corn hybrid seed.
Sterile inbred line in the breeding hybridized compound method of described high yield mandarin duck corn, sterility cross-bred kind of seed selection steps A is meant C type or the sterile inbred line of S type.
The sterile inbred line of C type in the breeding hybridized compound method of described high yield mandarin duck corn, sterility cross-bred kind of seed selection steps A is meant Rb type, Es type, the sterile inbred line of Bb type.
The sterile inbred line of S type in the breeding hybridized compound method of described high yield mandarin duck corn, sterility cross-bred kind of seed selection steps A is meant M type, R type and the sterile inbred line of 21A type.
Described high yield mandarin duck corn planting method can be educated crossbreed and is meant any ordinary maize crossbreed that blooms the same period with sterility cross-bred kind.
Described high yield mandarin duck corn planting method, sterility cross-bred kind and can educate that the crossbreed cover is broadcast or the mixed seeding ratio is 2～5: 1, field planting density is 3000～4500 strain/mus.
Described high yield mandarin duck corn planting method, a kind of sterile corn crossbreed of selection are the beautiful No. 9 sterility cross-bred kinds in Bb type Ji, and the educated crossbreed of selection is beautiful No. 12 of Ji.
The invention has the beneficial effects as follows: 1, volume increase: the mandarin duck corn hybrid seed of this method preparation of plant utilization, than the former ordinary maize crossbreed volume increase of plantation 14-22.88%; 2, simplified the corn breeding program, only needed to cultivate male sterile line, do not needed to cultivate and recover system, saved time, laborsaving.Main innovate point of the present invention is to select a pair of crossbreed, so called after mandarin duck crossbreed.
Description of drawings
Sterile corn hybrid seed breeding method flow chart of the present invention shown in Figure 1
Embodiment
Embodiment 1: the seed selection preparation of crossbreed
1. the transformation of sterile gene: the maternal inbred line Ji 15-22 with Ji beautiful No. 9 (its hybrid combination is 15-22 * Ji, Ji 35) was a male parent in 2000, and sterile inbred line Mo17 is a hybridization of female parent with C type Bb subspecies, obtains the sterile F of C type Bb subspecies
1Seed;
2. backcross: the winter in 2000 Hainan plantation step 1. in the F of transformation
1For seed, tasseling stage is pulled out all can educate strain, selects male sterile plant, does male parent with Ji 15-22 and backcrosses, and selects to have excellent economical character in the strain of backcrossing, and the plant fruit ear similar to Ji 15-22 reserve seed for planting, and obtains the sterile Bb Ji 15-22BC that comprises that 5 sisters are
1Seed notices that sisters system will gather in the crops respectively;
3. continuous backcross: the calendar year 2001 summer sowing plant step 2. in sterile Bb Ji 15-22BC
1Seed, the plantation respectively of sisters system, plant 5 row altogether, tasseling stage is pulled out all can educate strain, select the higher sisters system of male sterile degree, do male parent with Ji 15-22 again and carry out secondary and backcross, hocket in Hainan and locality so continuously and backcross for 5 times, obtain that 5 proterties are comparatively similar to backcross parent, sterile degree has reached 98%, and the sterile backcross progeny sisters with good economical character are Bb Ji 15-22BC
5
4. the assembly of the beautiful No. 9 sterility cross-bred kinds of homotype in Ji: step is planted in summer sowing in 2003, and to obtain sterile 5 sisters in 3. be backcross progeny seed Bb Ji 15-22BC
5, each sisters is plantation two row, plants 5 sisters altogether and be 10 row, plants the male parent Ji 35 of beautiful No. 9 of Ji simultaneously the same period, tasseling stage is all pulled out BC
5In educated strain, select male sterile sisters system, backcross with sterile strain continuation of wherein delegation and Ji 15-22 in each sisters system, directive breeding the offspring reserve seed for planting, the sterile strain of another row is then hybridized with inbred line Ji 35, every capable that 2 hybridize fruit ears in 5 row, obtain 10 hybridization fruit ears altogether, all be that No. 9 sterility cross-bred kinds of homotype Bb of Ji Yu are the beautiful No. 9 sterility cross-bred kinds in Bb type Ji;
5. the coordinate force of crossbreed test: the winter in 2003 Hainan plantation step 4. in No. 9 sterility cross-bred kinds of 5 Bb type Jis jade of becoming of seed selection, 2 hybridization fruit ear seeds that each crossbreed obtains mix back plantation 2 row, be numbered Bb type Ji respectively beautiful No. 9-1, beautiful No. 9-2 of Bb type Ji ... beautiful No. 9-5 of Bb type Ji, plantation simultaneously can be educated the crossbreed Ji beautiful No. 12, the loose powder phase pollinates for the beautiful No. 9 sterility cross-bred kinds in these 5 Bb type Jis with the pollen of beautiful No. 12 crossbreed in Ji fully blooming, wherein the beautiful No. 12 assembly output in Bb type Ji jade No. 9-3 and Ji are the highest, therefore select Bb type Ji to educate No. 9-3 for producing the sterility cross-bred kind in the mandarin duck crossbreed, be called for short the beautiful No. 9 sterility cross-bred kinds in Bb type Ji, and Bb type Ji is the mandarin duck crossbreed beautiful No. 12 with the Ji beautiful No. 9-3;
Embodiment 2: high yield mandarin duck maize planting
Utilize the 5. mandarin duck crossbreed of middle apolegamy of embodiment 1 step, in the beautiful No. 9 sterility cross-bred kinds in Bb type Ji with can educate beautiful No. 12 row in crossbreed Ji and carry out the field cover than 4: 1 ratio and broadcast, planting density is 4000 strain/mus, mu is produced 741.1 kilograms of corns, than similarity condition beautiful No. 9 volume increase 22.88% in plantation ordinary maize single cross hybrid Ji down.
Beautiful No. 9 sterility cross-bred kinds in Bb type Ji and ordinary maize single cross hybrid Ji are educated No. 9 except that fertility, and other features are identical.
Embodiment 3: the seed selection preparation of crossbreed
Embodiment 1 step 1. in the transformation of sterile gene be yellow with the sterile inbred line of C type Es subspecies four serve as the hybridization that female parent carries out early, obtain the sterile F of C type Es subspecies
1Seed; Embodiment 1 step 5. in coordinate force test used to educate crossbreed be Zheng Dan 958, beautiful No. 9 sterility cross-bred kinds in Es type Ji and Zheng Dan 958 are the mandarin duck crossbreed; All the other are identical with embodiment 1.
Embodiment 4: high yield mandarin duck maize planting
Carry out field cover with Zheng Dan 958 row than 3: 1 ratio in the beautiful No. 9 sterility cross-bred kinds in the Es type Ji described in the embodiment 3 and broadcast, the mandarin duck corn hybrid seed production ratio similarity condition of producing is planted beautiful No. 9 volume increase 16.66% in ordinary maize single cross hybrid Ji down.
Beautiful No. 9 sterility cross-bred kinds in Es type Ji and ordinary maize single cross hybrid Ji are educated No. 9 except that fertility, and other features are identical.
Embodiment 5: the seed selection preparation of crossbreed
Embodiment 1 described step 1. in the transformation of sterile gene be to be the hybridization that female parent carries out with the sterile inbred line C103 of S type R subspecies, obtain the sterile F of S type R subspecies
1Seed; Embodiment 1 step 5. in coordinate force test used to educate crossbreed single 20 for dredging, the beautiful No. 9 sterility cross-bred kinds in R type Ji are the mandarin duck crossbreed with dredging single 20.
Embodiment 6: high yield mandarin duck maize planting
Carry out field planting in 5: 1 ratios with the beautiful No. 9 sterility cross-bred kinds in the R type Ji described in the embodiment 5 and after dredging single 20 seed mixings, the mandarin duck corn hybrid seed production ratio similarity condition of producing plantation ordinary maize single cross hybrid Ji down increases production 20.22% beautiful No. 9.
Beautiful No. 9 sterility cross-bred kinds in R type Ji and ordinary maize single cross hybrid Ji are educated No. 9 except that fertility, and other features are identical.
Listed examples of the present invention is intended to set forth further the seed selection compound method of high yield mandarin duck maize planting and crossbreed, and scope of the present invention is not constituted any restriction.All can realize purpose of the present invention with the embodiment of the invention with via the described method of claims 1-8 of the present invention.
Claims (5)
1, a kind of high yield mandarin duck maize planting method is characterized in that a kind of sterile corn crossbreed and a kind of corn hybrid seed of educating are carried out that field cover is broadcast or the mixed seeding plantation; The seed selection of described sterile corn crossbreed may further comprise the steps:
The transformation of A, sterile gene: the maternal selfing with the ordinary maize single cross hybrid is a male parent, is that female parent is hybridized with a kind of sterile selfing, obtains first-filial generation F
1Sterile seed;
B, backcross: the maternal inbred line of the ordinary maize single cross hybrid of plantation steps A and the first-filial generation F that steps A obtains
1Seed, tasseling stage is selected male sterile plant, does male parent with the maternal inbred line of the ordinary maize single cross hybrid of steps A and backcrosses, and obtains the sterile BC that a plurality of sisters are
1Seed, sisters system gathers in the crops respectively;
C, continuous backcross: the maternal inbred line of the ordinary maize single cross hybrid of plantation steps A and the sterile BC that step B obtains
1Seed, sisters system is plantation respectively, tasseling stage is selected the higher sisters system of male sterile degree in the sisters system, does male parent with the maternal inbred line of the ordinary maize single cross hybrid of steps A and carries out secondary and backcross; Continuous backcross 5 times obtains the proterties a plurality of sterile sisters system comparatively identical with backcross parent;
The assembly of D, the sterility cross-bred kind of homotype: the seed that a plurality of sisters that continuous backcross obtains for 5 times among the plantation step C are, each sisters is plantation two row; The sterile strain of wherein delegation in each sisters system of tasseling stage continues to do male parent with the maternal inbred line of the ordinary maize single cross hybrid of steps A backcrosses, and directive breeding the offspring reserve seed for planting; The sterile strain of another row then with the male parent hybridization between selfed lines of the ordinary maize single cross hybrid of steps A, a plurality of male sterile hybrid combinations that obtain are the sterility cross-bred kind of homotype of the ordinary maize single cross hybrid of steps A;
The coordinate force test of E, sterility cross-bred kind: the sterility cross-bred kind that seed selection becomes among the plantation step D, and plant a kind of crossbreed that educates simultaneously; Each sterility cross-bred kind plantation 2 row repeats 2 times, and numbering; Pollinate for sterility cross-bred kind in the loose powder phase of blooming fully with the pollen that can educate crossbreed, select the sterility cross-bred kind of the present age, be the sterility cross-bred kind of using in the production of mandarin duck corn hybrid seed than the obvious effect of increasing production of the ordinary maize crossbreed of steps A; This sterility cross-bred kind and the educated crossbreed of being planted are a pair of mandarin duck corn hybrid seed.
2, high yield mandarin duck maize planting method according to claim 1 is characterized in that:
Sterile inbred line in the steps A is meant C type or the sterile inbred line of S type.
3, high yield mandarin duck maize planting method according to claim 2 is characterized in that:
The sterile inbred line of C type in the steps A is meant Rb type, Es type or the sterile inbred line of Bb type.
4, high yield mandarin duck maize planting method according to claim 2 is characterized in that:
The sterile inbred line of S type in the steps A is meant M type, R type or the sterile inbred line of 21A type.
5, high yield mandarin duck maize planting method according to claim 1 is characterized in that:
With the sterile corn crossbreed of mandarin duck corn hybrid seed with can educate corn hybrid seed and carry out that the field cover is broadcast or the mixed seeding plantation, planting proportion is 2～5: 1, and planting density is 3000～4500 strain/mus.
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| CNB2006100128123A CN100426949C (en) | 2006-06-12 | 2006-06-12 | High-yield twin-corn plantation and its cenospecies selective breeding method |
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| CNB2006100128123A CN100426949C (en) | 2006-06-12 | 2006-06-12 | High-yield twin-corn plantation and its cenospecies selective breeding method |
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| CN105454031A (en) * | 2015-12-02 | 2016-04-06 | 云南北玉种子科技有限公司 | Cultivation method of high-yield and high-resistance corn |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1692712A (en) * | 2005-05-19 | 2005-11-09 | 河北冀南种业有限公司 | Sterilization type cross breeding method for corn seeds |
| CN1709040A (en) * | 2005-07-14 | 2005-12-21 | 河北冀南种业有限公司 | Sterile maize hybrid seed production method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1692712A (en) * | 2005-05-19 | 2005-11-09 | 河北冀南种业有限公司 | Sterilization type cross breeding method for corn seeds |
| CN1709040A (en) * | 2005-07-14 | 2005-12-21 | 河北冀南种业有限公司 | Sterile maize hybrid seed production method |
Non-Patent Citations (1)
| Title |
|---|
| 粮饲专用玉米冀玉9 号的选育及特征特性. 张文英，郑积德等.河北农业科学，第8卷第1期. 2004 * |
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