CN101077182A - Technology for preparing functional dietary fiber through myrica ruba marc enzymolysis - Google Patents
Technology for preparing functional dietary fiber through myrica ruba marc enzymolysis Download PDFInfo
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- CN101077182A CN101077182A CNA2007100697186A CN200710069718A CN101077182A CN 101077182 A CN101077182 A CN 101077182A CN A2007100697186 A CNA2007100697186 A CN A2007100697186A CN 200710069718 A CN200710069718 A CN 200710069718A CN 101077182 A CN101077182 A CN 101077182A
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Abstract
The present invention discloses the technological process of enzymolyzing bayberry fruit dregs to prepare functional diet fiber. The technological process includes the following steps: defatting bayberry fruit dregs, adding pH 4.5-5.5 phosphate buffering solution in 15-20 ml/g and 0.1-0.5 % enzyme concentration and 5-10 U/ml enzyme activity cellulolytic enzyme solution, enzymolysis at 100-120 rpm and 55-60 deg.c for 1-2 hr, alcohol separating, and vacuum drying to obtain functional diet fiber. The simple technological process changes waste into valuable functional diet fiber and is environment friendly.
Description
Technical field
The invention belongs to agricultural byproducts discarded object deep processing field, relate to a kind of technology preparing functional dietary fiber through myrica ruba marc enzymolysis discarded after the red bayberry processing.
Background technology
Red bayberry (Myrica rubra Sieb.et Zucc) originates in China, is a kind of characteristic fruit of China.But because red bayberry listing is concentrated, supply time is short, and is difficult for fresh-keepingly, and the rate of eating raw of red bayberry has only 20～30%, and major part is used to produce red bayberry processing industries such as red bayberry fruit wine, red bayberry juice.But 100 tons of arbutuses of every deep processing will produce the myrica ruba marc about 3 tons.And fresh myrica ruba marc is very easily rotten, when causing the wasting of resources, and certain broken evil of bringing also for the ecological environment in red bayberry producing region.Advocate resources conservation now, efficiently utilize and the overall background of recycling economy under, carry out red bayberry utilization of waste as resource research and seem particularly important.At present, myrica ruba marc also of no use improves soluble dietary fibre content in the pomace, to prepare the technology of functional myrica ruba marc dietary fiber by cellulase hydrolysis.
Summary of the invention
The objective of the invention is to work out a kind of technology of preparing functional dietary fiber through myrica ruba marc enzymolysis.
What the present invention will solve is that existing myrica ruba marc prepares the technology of preparing problem that exists in the functional dietary fiber.
Technical scheme of the present invention may further comprise the steps:
(1) myrica ruba marc is prepared: the myrica ruba marc after red bayberry is squeezed the juice is directly standby; Or pulverize through 60～80 ℃ of temperature oven dry back, mistake 80～100 mesh sieves, standby;
(2) organic solvent lixiviate: the myrica ruba marc in the step (1) is placed in the closed container, seal extracting with organic solvent, to slough the lipid in the myrica ruba marc, the organic solvent addition is as the criterion to flood myrica ruba marc, after the time that lixiviate needs, organic solvent is reclaimed in press filtration, and dry myrica ruba marc is removed residual organic solvent in the myrica ruba marc;
(3) cellulase hydrolysis: in the myrica ruba marc behind degreaser drying, add the phosphate buffer 1 5～20mL/g of pH4.5～5.5 and enzyme concentration and be 0.1～0.5%, enzyme work is the cellulase solution of 5～10u/mL, under 100～120rpm, 55～60 ℃, enzymolysis 1～2 hour, be warmed up to 100 ℃ again, keep 5 minutes enzymes that go out;
(4) alcohol is analysed: the enzymolysis liquid behind the above-mentioned enzymolysis, carry out Separation of Solid and Liquid, filter residue is with 60～70 ℃ hot water injection, merging filtrate, filtrate merges the precipitation and the filter residue precipitation of filtering in the filtrate with 60 ℃ of 4 times of volumes, 95% alcohol chromatography soluble dietary fiber, is always precipitated;
(5) vacuum drying: in temperature is that 60～70 ℃, pressure are under the condition of 0.06MPa～0.08MPa, and the total precipitation vacuum drying in the step (4) to moisture below 5%, is obtained waxberry dregs functional dietary fiber finished product.
Advantage of the present invention: the one, because the present invention has adopted zymotechnic, the extraction conditions gentleness has kept the active material in the myrica ruba marc effectively.Use the present invention and can obtain that productive rate is higher, the measured myrica ruba marc dietary fiber of matter.After testing, waxberry dregs dietary fiber productive rate reaches 80～90% in the myrica ruba marc dietary fiber finished product that the present invention makes, retention ability, and the swellability height is good myrica ruba marc functional food additives.The 2nd, the present invention is simple, convenient, does not need high temperature, high pressure, energy savings, low, the constant product quality of production cost.The 3rd, the present invention is to be raw material with a large amount of discarded pomaces of producing in the red bayberry process, well solved discarded myrica ruba marc very easily rotten, rot and the environmental pollution and the wasting of resources problem that cause.The 4th, the myrica ruba marc functional dietary fiber that the present invention makes helps to solve the problem of obesity that causes because of the unreasonable diet of people, because the insoluble diedairy fiber in this myrica ruba marc functional dietary fiber is given stomach a kind of " feeling of repletion ", alleviate vigorous appetite, play the effect of fat-reducing, and soluble dietary fiber can be controlled cholesterol, help people's digestive function, alleviate hunger.
The specific embodiment
Below in conjunction with example the present invention is further described.
Embodiment 1:
Myrica ruba marc is prepared: the myrica ruba marc after red bayberry is squeezed the juice is directly standby.
The organic solvent lixiviate: take by weighing folding oven dry dry powder 250g (myrica ruba marc after squeezing the juice through 60～80 ℃ of temperature oven dry pulverize, dry powder after crossing 80～100 mesh sieves is heavy) the pomace of red bayberry after squeezing the juice, be placed in the closed container, seal extracting with ether, to slough the lipid in the myrica ruba marc, the ether addition is as the criterion to flood pomace.After the lixiviate 24 hours, organic solvent is reclaimed in press filtration, and dry myrica ruba marc is removed residual organic solvent in the myrica ruba marc, reclaims ether.Here dry the weight of dry powder
Cellulase hydrolysis: the phosphate buffer 20mL/g (myrica ruba marc dry matter weight) that adds pH=4.5 in the myrica ruba marc behind degreaser drying, enzyme concentration is 0.1%, enzyme is lived and is the cellulase solution of 6u/mL, at 120rpm, in 55 ℃ of water-baths, enzymolysis 1 hour is warmed up to 100 ℃ again, keeps 5 minutes enzymes that go out.
Alcohol is analysed: the enzymolysis liquid after the degraded carries out Separation of Solid and Liquid, and filter residue is with 60～70 ℃ of hot water injections, merging filtrate.Filtrate is with 60 ℃, 95% alcohol chromatography soluble dietary fiber of 4 times of volumes.Merge precipitation and filter residue precipitation in the filtered fluid, always precipitated.
Vacuum drying: at 65 ℃, pressure is under the 0.06MPa condition, and the vacuum drying of total precipitation to moisture below 5%, is obtained myrica ruba marc functional dietary fiber finished product.
Above-mentioned cellulolytic enzyme is meant can cellulolytic cellulase or dextranase.
Above-mentioned myrica ruba marc is meant the Waxberry enucleation pomace of any kind.
Above-mentioned enzyme work is meant that under 40 ℃ of temperature, pH4.2 condition interior hydrolyzed carboxymethylcellulo, e sodium substrate produces the enzyme amount that is equivalent to 1 microgram glucose in 1 minute, is 1 cellulase activity unit.
In the myrica ruba marc sample after above-mentioned former red bayberry is squeezed the juice, dietary fiber content is 74.68%, and soluble dietary fiber is 9.69%, and insoluble diedairy fiber is 64.99%.After degraded, dietary fiber content is 84.35% in the finished product, soluble dietary fiber 25.30%, insoluble diedairy fiber 61.42%, retention ability 500.20%, swellability 4.3ml/g.
Total dietary fiber, insoluble diedairy fiber, solubility meals assay adopt Prosky method (enzyme-gravimetric method) in the above-mentioned dietary fiber.
Embodiment 2:
Reclaim myrica ruba marc, 60 ℃ of oven dry are pulverized, and cross 80 mesh sieves.
The organic solvent lixiviate: take by weighing dry powder 300g, be placed in the closed container, seal extracting with ether, to slough the lipid in the myrica ruba marc, the ether addition is as the criterion to flood pomace.After the lixiviate 25 hours, organic solvent is reclaimed in press filtration, and dry myrica ruba marc is removed residual organic solvent in the myrica ruba marc, reclaims ether.
Cellulase hydrolysis: the phosphate buffer 20mL/g (myrica ruba marc dry matter weight) that adds pH=5.5 in the myrica ruba marc behind degreaser drying, enzyme concentration is 0.3%, enzyme is lived and is the cellulase solution of 6u/mL, at 120rpm, in 55 ℃ of water-baths, enzymolysis 1 hour is warmed up to 100 ℃ again, keeps 5 minutes enzymes that go out.
Alcohol is analysed: the enzymolysis liquid after the degraded carries out Separation of Solid and Liquid, and filter residue is with 60～70 ℃ of hot water injections, merging filtrate.Filtrate is with 60 ℃, 95% alcohol chromatography soluble dietary fiber of 4 times of volumes.Merge precipitation and filter residue precipitation in the filtered fluid, always precipitated.
Vacuum drying: at 60 ℃, pressure is under the 0.07MPa condition, and the vacuum drying of total precipitation to moisture below 5%, is obtained myrica ruba marc functional dietary fiber finished product.
Above-mentioned cellulolytic enzyme is meant can cellulolytic cellulase or dextranase.
Above-mentioned myrica ruba marc is meant the Waxberry enucleation pomace of any kind.
Above-mentioned enzyme work is meant that under 40 ℃ of temperature, pH4.2 condition interior hydrolyzed carboxymethylcellulo, e sodium substrate produces the enzyme amount that is equivalent to 1 microgram glucose in 1 minute, is 1 cellulase activity unit.
In the above-mentioned former myrica ruba marc sample, dietary fiber content is 74.68%, and soluble dietary fiber is 9.69%, and insoluble diedairy fiber is 64.99%.After degraded, dietary fiber content is 86.70% in the finished product, soluble dietary fiber 26.30%, insoluble diedairy fiber 60.40%, retention ability 548.20%, swellability 4.5ml/g.
Total dietary fiber, insoluble diedairy fiber, solubility meals assay adopt Prosky method (enzyme-gravimetric method) in the above-mentioned dietary fiber.
Embodiment 3:
Reclaim myrica ruba marc, 60 ℃ of oven dry are pulverized, and cross 80 mesh sieves.
The organic solvent lixiviate: take by weighing dry powder 500g, be placed in the closed container, seal extracting with ether, to slough the lipid in the myrica ruba marc, the ether addition is as the criterion to flood pomace.After the lixiviate 23 hours, organic solvent is reclaimed in press filtration, and dry myrica ruba marc is removed residual organic solvent in the myrica ruba marc, reclaims ether.
Cellulase hydrolysis: the phosphate buffer 20mL/g (myrica ruba marc dry matter weight) that adds pH=5 in the myrica ruba marc behind degreaser drying, enzyme concentration is 0.5%, enzyme is lived and is the cellulase solution of 8u/mL, at 120rpm, in 60 ℃ of water-baths, enzymolysis 2 hours is warmed up to 100 ℃ again, keeps 5 minutes enzymes that go out.
Alcohol is analysed: the enzymolysis liquid after the degraded carries out Separation of Solid and Liquid, and filter residue is with 60～70 ℃ of hot water injections, merging filtrate.Filtrate is with 60 ℃, 95% alcohol chromatography soluble dietary fiber of 4 times of volumes.Merge precipitation and filter residue precipitation in the filtered fluid, always precipitated.
Vacuum drying: at 60 ℃, pressure is under the 0.08MPa condition, and the vacuum drying of total precipitation to moisture below 5%, is obtained myrica ruba marc functional dietary fiber finished product.
Above-mentioned cellulolytic enzyme is meant can cellulolytic cellulase or dextranase.
Above-mentioned myrica ruba marc is meant the Waxberry enucleation pomace of any kind.
Above-mentioned enzyme work is meant that under 40 ℃ of temperature, pH4.2 condition interior hydrolyzed carboxymethylcellulo, e sodium substrate produces the enzyme amount that is equivalent to 1 microgram glucose in 1 minute, is 1 cellulase activity unit.
In the above-mentioned former myrica ruba marc sample, dietary fiber content is 74.68%, and soluble dietary fiber is 9.69%, and insoluble diedairy fiber is 64.99%.After degraded, dietary fiber content is 88.63% in the finished product, soluble dietary fiber 28.30%, insoluble diedairy fiber 62.40%, retention ability 598.60%, swellability 5.0ml/g.
Total dietary fiber, insoluble diedairy fiber, solubility meals assay adopt Prosky method (enzyme-gravimetric method) in the above-mentioned dietary fiber.
The essence of cellulase degradation myrica ruba marc of the present invention is the crude fibre in the activation pomace, the fibrous inside constituent is optimized and reorganization, degree of crystallinity and purity improve, can produce simultaneously a large amount of exposed new surfaces, improve its absorption, dispersion, conformality, cellulosic retention ability and expansive force are improved rapidly.Because myrica ruba marc multifunctional diet fibre with the exploitation of cellulose enzyme process, kept major part and had Soluble Fiber and half fiber, so the myrica ruba marc multifunctional diet fibre finished product that obtains has good physiological function at aspects such as control obesity, hypertension, hyperglycaemia, removing external source harmful substances than Johnson ﹠ Johnson's reason function.
Claims (6)
1, the technology of preparing functional dietary fiber through myrica ruba marc enzymolysis, the processing step of this technology is as follows:
(1) myrica ruba marc is prepared: the myrica ruba marc after red bayberry is squeezed the juice is directly standby; Or pulverize through 60～80 ℃ of temperature oven dry back, mistake 80～100 mesh sieves, standby;
(2) organic solvent lixiviate: the myrica ruba marc in the step (1) is placed in the closed container, seal extracting with organic solvent, to slough the lipid in the myrica ruba marc, the organic solvent addition is as the criterion to flood myrica ruba marc, after the time that lixiviate needs, organic solvent is reclaimed in press filtration, and dry myrica ruba marc is removed residual organic solvent in the myrica ruba marc;
(3) cellulase hydrolysis: in the myrica ruba marc behind degreaser drying, add the phosphate buffer 1 5～20mL/g of pH4.5～5.5 and enzyme concentration and be 0.1～0.5%, enzyme work is the cellulase solution of 5～10u/mL, under 100～120rpm, 55～60 ℃, enzymolysis 1～2 hour, be warmed up to 100 ℃ again, keep 5 minutes enzymes that go out;
(4) alcohol is analysed: the enzymolysis liquid behind the above-mentioned enzymolysis, carry out Separation of Solid and Liquid, filter residue is with 60～70 ℃ hot water injection, merging filtrate, filtrate merges the precipitation and the filter residue precipitation of filtering in the filtrate with 60 ℃ of 4 times of volumes, 95% alcohol chromatography soluble dietary fiber, is always precipitated;
(5) vacuum drying: in temperature is that 60～70 ℃, pressure are under the condition of 0.06MPa～0.08MPa, and the total precipitation vacuum drying in the step (4) to moisture below 5%, is obtained waxberry dregs functional dietary fiber finished product.
2, the technology of claims 1 described preparing functional dietary fiber through myrica ruba marc enzymolysis is characterized in that cellulolytic enzyme is meant cellulolytic cellulase of energy or dextranase.
3, the technology of claims 1 described preparing functional dietary fiber through myrica ruba marc enzymolysis is characterized in that myrica ruba marc is meant the Waxberry enucleation pomace of any kind.
4, the technology of claims 1 described preparing functional dietary fiber through myrica ruba marc enzymolysis is characterized in that organic solvent is an ether.
5, the technology of claims 1 described preparing functional dietary fiber through myrica ruba marc enzymolysis, the time that it is characterized in that the lixiviate needs is 22～26 hours.
6, the technology of claims 1 described preparing functional dietary fiber through myrica ruba marc enzymolysis, it is characterized in that enzyme work is meant under 40 ℃ of temperature, pH4.2 condition, hydrolyzed carboxymethylcellulo, e sodium substrate in 1 minute, generation is equivalent to the enzyme amount of 1 microgram glucose, is 1 cellulase activity unit.
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| CN101744230B (en) * | 2010-01-26 | 2012-05-16 | 浙江扬眉饮品有限公司 | Method for preparing red bayberry dietary fiber powder |
| CN102524701A (en) * | 2012-02-17 | 2012-07-04 | 中国农业大学 | Fruit residue dietary fiber and preparation method thereof |
| CN104187456A (en) * | 2014-07-11 | 2014-12-10 | 合肥工业大学 | Technical method for extracting dietary fiber from pear residue |
| CN104886605A (en) * | 2015-05-15 | 2015-09-09 | 张志旭 | Extraction and preparation method for high-antioxidant dietary fiber |
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| CN102524701B (en) * | 2012-02-17 | 2013-09-18 | 中国农业大学 | Fruit residue dietary fiber and preparation method thereof |
| CN105077287A (en) * | 2014-05-06 | 2015-11-25 | 阿拉善盟万铭生物制品有限公司 | Cynomorium songaricum dietary fiber powder, preparation method and applications thereof |
| CN104187456A (en) * | 2014-07-11 | 2014-12-10 | 合肥工业大学 | Technical method for extracting dietary fiber from pear residue |
| CN104886605A (en) * | 2015-05-15 | 2015-09-09 | 张志旭 | Extraction and preparation method for high-antioxidant dietary fiber |
| CN105707235A (en) * | 2016-01-29 | 2016-06-29 | 广西壮族自治区农业科学院农产品加工研究所 | Intestine moistening and bowel relaxing yogurt drink and preparation method thereof |
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| CN106174600A (en) * | 2016-07-19 | 2016-12-07 | 唐春艳 | Method of dietary fiber dry powder and products thereof is prepared with marc for raw material |
| CN106993580A (en) * | 2017-04-28 | 2017-08-01 | 广西乐业康辉生态养殖专业合作社 | It is a kind of to improve the chicken-raising method of chicken meat |
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| CN110101040A (en) * | 2018-12-27 | 2019-08-09 | 长沙理工大学 | A kind of preparation method of the instant lyophilized powder of red bayberry |
| CN110063504A (en) * | 2019-04-29 | 2019-07-30 | 陕西科技大学 | A kind of natural dietary fiber replenishers and preparation method thereof with effect for reducing fat |
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