CN101057609A

CN101057609A - Long-acting sour milk containing beneficial bacteria factor and its producing method

Info

Publication number: CN101057609A
Application number: CN 200710099488
Authority: CN
Inventors: 吴秀英; 李洪亮; 母智深; 陈伟; 李树森
Original assignee: Inner Mongolia Mengniu Dairy Group Co Ltd
Current assignee: Inner Mongolia Mengniu Dairy Group Co Ltd
Priority date: 2007-05-22
Filing date: 2007-05-22
Publication date: 2007-10-24
Anticipated expiration: 2027-05-22
Also published as: CN101057609B

Abstract

The invention discloses a long-effect yoghourt and process for preparation, wherein the yoghourt is prepared from the following raw materials (by weight portions): milk 50-100, isomaltose hypgather 0. 01-20. 00 and stabilizing agent 0. 2-8. 0. The nourishing constituents in the yoghourt can be preserved, and the storage period of the product can reach longer than six months.

Description

A kind of long-acting sour milk and production method thereof that contains beneficial bacteria factor

Technical field

The present invention relates to a kind of long-acting sour milk and production method thereof that contains beneficial bacteria factor.

Background technology

Yoghurt in the market is take sweet milk or dairy products as raw material, adds sugar, strain fermentation forms. Protein content is not less than the yoghurt that is called of 2.3% (m/V) in the finished product. Long-acting sour milk namely after heat treatment passes through the fermented yoghourt of aseptic packaging again, but its maximum characteristics be under the normal temperature state holding time be not less than 60 days, and mouthfeel and taste and conventional sour milk are basic identical. The main component of long-acting yoghurt is milk, water, white granulated sugar, thickener, emulsifying agent, acidity regulator and flavorant, according to the requirement of people to health, in product, add vitamin, mineral matter, amino acid, fruit grain, fruit juice, jam or one or more nutriments of nut granule. World dairy products federation had formulated standard to long-acting sour milk in 2003, so it is acknowledged as a kind of certified milk goods of standard. Its trade name remains " sour milk yogurt ", but will be titled with " heat treatment thermized " or other prefix. Nutritive value about long-acting sour milk, world dairy products joint committee is finalized: the nutrition of sour milk is not only from viable bacteria, the metabolite that produces in the Yoghourt fermentation process also has high nutritive value, exists even without viable bacteria, and sour milk remains senior nutritional health food. Concerning the huge lactose in Asia was not suitable with the crowd, sour milk particularly long-acting sour milk was significant. Therefore, such dairy products had obtained extensive concern in recent years, long-acting sour milk has not only kept the nutritional labeling of sour milk, the shelf-life of product was reached more than six months, enlarged the sales range of product, thereby made the people that are in the mountain village that the geographical position is remote, communications and transportation is inconvenient can enjoy multiple tastes, be of high nutritive value and drink easily yoghurt.

Bifidobacterium is typical beneficial bacteria in the human body intestinal canal, and its growth and breeding is applied in people's the whole life course. Bifidobacterium growth and breeding under anaerobic environment produces a large amount of lactic acid, reduces the pH of system value and gut flora is changed, and suppresses and kills enteric pathogenic bacteria, makes flora maintenance normal equilibrium. Yet the quantity of Bifidobacterium is still a technical barrier up to now in the raising human body. This is because Bifidobacterium is the anaerobic type flora, in air, be difficult to survival, the acidity of adding hydrochloric acid in gastric juice is very strong, the overwhelming majority all has been killed during Bifidobacterium process stomach, only have few part to arrive in the intestines, thereby make its beneficial functional to human body be difficult to embody, and the flora of the Bifidobacterium of different people is also incomplete same, namely allow to replenish the Bifidobacterium that lives, can not adapt to all crowds.

Oligoisomaltose (IMO) is a kind of of starch sugar, main component is by α-1, the functional oligose that the isomaltose (IG2) of 6-glycosidic bond combination, panose (P), Isomaltotriose (IG3) and tetrose above (GN) are combined into. Can be divided into two kinds in oligoisomaltose slurry and oligoisomaltose powder by form. Syrup is colourless or light yellow, transparent thick liquid, and sweet taste is soft, and free from extraneous odour is without the visible impurity of twenty-twenty vision. Icing Sugar is white amorphous powder, and sweet taste is soft, and free from extraneous odour is without the visible impurity of twenty-twenty vision. The basic composition of different purity oligoisomaltose (IMO) is as shown in table 1. The production technology of oligoisomaltose (IMO) is roughly: the glycosides-enzyme that goes out-essence filter (micro-filtration)-ion-exchange-concentrated-finished product is filtered-turned to the exquisite cornstarch-liquefaction-saccharification-enzyme-essence of going out.

The basic composition of table 1 different purity oligoisomaltose (IMO)

Project	Requirement
	Requirement				The IMO-50 type		The IMO-90 type
	Syrup	Icing Sugar	Syrup	Icing Sugar	The IMO-50 type		The IMO-90 type
	Syrup	Icing Sugar	Syrup	Icing Sugar	IMO content (accounting for dry) % 〉=	50		90
IG ₂+P+IG ₃Content (accounting for dry) % 〉=	35		45		IMO content (accounting for dry) % 〉=	50		90
IG ₂+P+IG ₃Content (accounting for dry) % 〉=	35		45		Dry (solid content)/% 〉=	75	-	75	-
Moisture/%≤	-	5	-	5	Dry (solid content)/% 〉=	75	-	75	-
Moisture/%≤	-	5	-	5	pH	4.0-6.0
Transmittance/% 〉=	95	-	95	-	pH	4.0-6.0
Transmittance/% 〉=	95	-	95	-	Solubility/% 〉=	-	99	-	99
Sulfated ass/%≤	0.3				Solubility/% 〉=	-	99	-	99
Sulfated ass/%≤	0.3				Arsenic/(mg/kg)	0.5
Plumbous/(mg/kg)	0.5				Arsenic/(mg/kg)	0.5
Plumbous/(mg/kg)	0.5				Total plate count/[cfu/ (g or mL)]≤	1500
Coliform/[MPN/ (100g or 100mL)]≤	30				Total plate count/[cfu/ (g or mL)]≤	1500
Coliform/[MPN/ (100g or 100mL)]≤	30				Pathogenic bacteria (salmonella)	Must not detect

Summary of the invention

The purpose of this invention is to provide a kind of oligoisomaltose (IMO) that contains, can promote long-acting sour milk and the production method thereof of beneficial bacterium in the body-Bifidobacterium propagation.

Long-acting sour milk provided by the present invention, it is made by the raw material of the component that contains leavening and following weight portion:

Milk 50-100

Oligoisomaltose 0.01-20.00

Stabilizing agent 0.2-8.0.

Wherein, the content of described oligoisomaltose is preferably the 0.01-15 weight portion, is powdery or liquid, and purity is greater than 50%, pure taste, similar sucrose.

Described oligoisomaltose (IMO) is a kind of of starch sugar, and main component is by α-1, the isomaltose (IG of 6-glycosidic bond combination₂), panose (P), Isomaltotriose (IG₃) and the above (G of tetrose_N) functional oligose that is combined into. Can be divided into two kinds in oligoisomaltose slurry and oligoisomaltose powder by form. Syrup is colourless or light yellow, transparent thick liquid, and sweet taste is soft, and free from extraneous odour is without the visible impurity of twenty-twenty vision. Icing Sugar is white amorphous powder, and sweet taste is soft, and free from extraneous odour is without the visible impurity of twenty-twenty vision. The basic composition of different purity oligoisomaltose (IMO) is as shown in table 1. The production technology of oligoisomaltose (IMO) is roughly: the glycosides-enzyme that goes out-essence filter (micro-filtration)-ion-exchange-concentrated-finished product is filtered-turned to the exquisite cornstarch-liquefaction-saccharification-enzyme-essence of going out.

Described milk is defined as: common cow's milk comprises full-cream, degreasing and half skimmed milk; Or with the formulated recombined milk of other components of milk powder, cream, protein hydrolysate, whey powder or cow's milk.

Described stabilizing agent is emulsifying agent or thickener, or is combined by emulsifying agent and thickener, and wherein, the ratio of weight and number of emulsifying agent and thickener is 1: 1-60; Described emulsifying agent can be selected from a kind of or its several combinations in single stearic fat glyceride, sucrose fatty ester, fatty acid glyceride and the polyglycerol ester; Thickener can be selected from a kind of or its several combinations in sodium carboxymethylcellulose, carragheen, gelatin, microcrystalline cellulose, gellan gum, xanthans, propylene glycol alginate, konjac glucomannan, starch, albumen powder, guar gum, D-glucose, agar, locust bean gum and the pectin.

Bacterial classification content can be 10 in the described leavening⁶-10 ⁸The CFU/100g raw material.

One or more the combination of the optional streptococcus thermophilus of described bacterial classification, streptococcus lactis, streptococcus cremoris, lactobacillus acidophilus, lactobacillus bulgaricus, missible oil streptococcus, the bacterium that produces aromatic substance, lemon leukonid, the bright string strain of pentose bacterium, diacetyl streptococcus lactis, thermophilic lactobacillus, Lactobacillus casei, Bifidobacterium, bifidobacterium bifidum, bifidobacterium longum, bifidobacterium infantis, bifidobacterium breve.

In order to improve the mouthfeel of sour milk, can contain 0-0.2 weight portion acidity regulator in the described long-acting sour milk, described acidity regulator can be the one or more combination in lactic acid, citric acid, phosphoric acid, tartaric acid and malic acid and the salt thereof.

In order to improve the mouthfeel of sour milk, contain white granulated sugar and 0-5 weight portion syrup or the sweetener of 3.0-15.0 weight portion in the described long-acting sour milk. Described sweetener can be acesulfame-K, Aspartame, sweet, the Sucralose of knob, and described syrup can be the one or more combination in fructose and the HFCS. Conversion is the white granulated sugar pol when calculating pol.

Abundanter for the nutrition that makes sour milk, can contain the trace element of fruit juice, jam, fruit grain, nut granule or the 0.01-0.5 weight portion of 0-20 weight portion in the described long-acting sour milk.

Described fruit grain can be the one or more combination in the various fruit-vegetable granules such as tomato particle, peach particle, coconut palm fruit granule, orange fruit grain, mango fruit grain, grape fruit, pawpaw fruit grain, pineapple sarcocarp grain, strawberry fruit grain, celery particle, the operatic circle grain, cucumber particle, radish kind particle and aloe gel particle; Usually select granular garden stuff grain or the slurry class fruit and vegetable of diameter 1-20mm, or length is that 1-20mm, width are the fibrous fruit and vegetable of 1-10mm.

Described fruit juice can be the one or more combination in the various Juices such as cider, strawberry juice, blueberry juice, Chinese flowering quince juice, bananas juice, orange juice, pineapple juice, mango juice, tomato juice, carrot juice, asparagus juice, spinach juice, peach juice, apricot juice, pear juice, grape juice, asparagus juice, kiwi-fruit juice, Lychee juice, lemon juice and Coconut Juice.

Described jam can be the one or more combination in the various jam such as apple jam, strawberry jam, blueberry sauce, Chinese quince soy sauce, banana sauce, orange sauce, pineapple jam, mango chutney, catsup, carrot sauce, asparagus sauce, spinach sauce, peach butter, apricot sauce, pears sauce, grape sauce, aloe paste, kiwi-fruit jam, lichee sauce, jam with lemon and coconut palm sauce.

Nut granule is selected one or more of peanut particle, fibert particle, walnut particle, sunflower seeds particle, almond particle, American pistachios particle. It is the spherical particle of 1-18mm that particle is selected diameter, and perhaps length is 1-20mm, the particle of width 1-12mm.

Described trace element can be vitamin A, vitamin B complex, vitamin D, vitamin C, vitamin E, calcium lactate, taurine, folic acid, nicotinic acid, choline, ferrous sulfate, ironic citrate, zinc sulfate, zinc gluconate, sodium selenite, magnesium sulfate, magnesium gluconate and l-cn etc. to the one or more combination in human body beneficial's the trace element.

In order to improve the local flavor of sour milk, the flavoring essence that also contains the 0.01-0.4 weight portion in the described long-acting sour milk, described flavoring essence can be the one or more combination in sour milk essence, milk flavour, orange essence, grape essence, peach essence, cocoanut flavour, strawberry essence, lychee flavor, flavoring apple essence, close melon essence, mango essence, passionflower essence, grapefruit essence, lemon extract, blueberry essence, pawpaw essence, flavoring banana essence, flavoring pineapple essence, tomato flavour, carrot essence, asparagus essence, spinach essence, apricot essence, pear essence, kiwi flavor, vanilla, flavoring rose essence, chocolate essence, walnut essence, wheat essence and the cherry essence.

The present invention contains the long-acting sour milk production method of beneficial bacteria factor, comprises the steps:

May further comprise the steps:

1) with sweetener, oligoisomaltose and the stabilizing agent of described weight portion, mixes with milk;

2) carrying out sterilization, cooling, the described leavening of adding ferments;

3) acidity regulator, flavoring essence and/or the sweetener of the described weight portion of interpolation after the fermentation ends, sterilization obtains long-acting sour milk.

Long-acting sour milk of the present invention contains oligoisomaltose (IMO), can promote beneficial bacterium in the body-Bifidobacterium propagation.

Long-acting sour milk of the present invention is compared with existing like product, has the following advantages and useful effect: 1) the present invention has improved the quantity (mainly for the acid strong crowd of hydrochloric acid in gastric juice) of Bifidobacterium in the human body; 2) this product has been broken through original sour milk products structure and prescription composition, has increased the new varieties of product; 3) this product has not only kept the nutritional labeling of sour milk, the shelf-life of product was reached more than two months, enlarged the sales range of product, thereby made the people that are in the mountain village that the geographical position is remote, communications and transportation is inconvenient can enjoy multiple tastes, be of high nutritive value and drink easily yoghurt. 4) interpolation of fruit juice, jam, fruit grain or nut granule has realized the combination of fruit vitamin, mineral matter, cellulose and milk protein, makes the nutrition of product more reasonable, more balanced; 5) fruit juice, jam or the interpolation of fruit grain in long-acting sour milk have promoted the development of fruits and vegetables industries, and the fruit resource of China's abundant is fully used, and its economic benefit and social benefit are very considerable.

The specific embodiment

Method therefor is conventional method if no special instructions among the following embodiment, and described degree is the quality percentage composition if no special instructions.

The preparation of embodiment 1, beneficial bacteria factor long-acting sour milk

Raw material:

Milk: protein: 2.98%, fat: 3.02%, non-fat solid: 8.55%.

White granulated sugar: meet country-level standard.

HFCS: F 〉=42.0%, 5%.

Sucrose fatty ester: Mitsubishi, HLB are 7.

Strawberry essence: green brilliant spices Co., Ltd, model: k001.

Starch: Co., Ltd is made a concerted effort to tie up in Guangzhou, model: M98.

Agar: Co., Ltd is made a concerted effort to tie up in Guangzhou, model: H65.

Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong Baolingbao Biotechnology Co., Ltd., model: FM500, for powdery.

Leavening: Sha Pu looks trade Co., Ltd's (model: OI654 is comprised of streptococcus thermophilus and lactobacillus bulgaricus, and the ratio of its viable count is 4: 6).

Pure water, lactic acid and citric acid all meet national standard.

Prepare beneficial bacteria factor long-acting sour milk of the present invention, may further comprise the steps:

1) by following composition of raw materials feeding (in 1 ton): 700.0 kilograms in milk, 95.0 kilograms of white granulated sugars, 50.0 kilograms of HFCSs, 18 kilograms of oligoisomaltoses (IMO), 1.6 kilograms in agar, 3.2 kilograms of sodium carboxymethylcelluloses, 10.0 kilograms of starch, 2 kilograms of sucrose fatty esters, leavening 10⁸CFU/100g, 0.3 kilogram of strawberry essence, 119.4 kilograms of pure water

2) agar, sucrose fatty ester, sodium carboxymethylcellulose and starch and white granulated sugar are mixed, be heated in 60 ℃ the milk, insulation dissolving 25 minutes, be cooled to 30 ℃ through cold drawing, add again and fill in the material-compound tank of HFCS, oligoisomaltose (IMO) and water fully stirring.

3) being warmed up to 40 ℃, pressure is under the 25Mp mixed liquor to be carried out homogeneous.

4) with step 3) feed liquid carry out sterilization in 92 ℃, 5 minutes.

5) add bacterial classification after being cooled to 37 ℃ after the sterilization, fermented 7 hours.

6) add strawberry essence after the fermentation and stir, after stirring, carry out 70 ℃, sterilization in 10 minutes is cooled to 30 ℃, sterile filling.

This sour milk contains beneficial bacteria factor (oligoisomaltose (IMO), it detects the check according to QB/T2491) can improve intestinal environment, promotes the growth of beneficial bacterium. In this sour milk the metabolite that produces in the sweat is arranged also simultaneously, be conducive to absorption of human body, make the nutrition of this sour milk abundanter.

The preparation of embodiment 2, beneficial bacteria factor long-acting sour milk

Raw material:

Milk: protein: 3.06%, fat: 3.13%, non-fat solid: 8.59%.

Albumen powder: WPC is 50, and dosage is 20 kilograms, and the synthetic industry in Guangzhou has Co., Ltd, model: N487.

White granulated sugar: meet country-level standard.

Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong Baolingbao Biotechnology Co., Ltd., model is Q756.

Fruit juice: inspissated juice, apple juice system pH3.7, pol 35BX.

Pectin: Shanghai shuttle ocean food development limited company, model: E102; Propylene glycol alginate: the inferior methylene blue in Beijing Trade Development Co., Ltd of speeding, model: R203; Gelatin: the special food science and technology of upper Haikang Mil Co., Ltd, model: T105; Locust bean gum: already trade Co., Ltd, model: T302 are believed in the Qinhuangdao; Flavoring apple essence: limit, field, Shanghai spices Co., Ltd, model w100.

Pure water meets national standard.

Be prepared as follows long-acting sour milk:

1) by following composition of raw materials feeding (in 1 ton): 500.0 kilograms in milk, 34 kilograms of whole milk powders, 2.0 kilograms of albumen powders, 95.0 kilograms of white granulated sugars, 15.0 kilograms of oligoisomaltoses (IMO), 15 kilograms of fruit juice, 3.0 kilograms of pectin, 1.0 kilograms of propylene glycol alginates, 4.0 kilograms in gelatin, 0.5 kilogram of locust bean gum, leavening 10⁷CFU/100g, 2.0 kilograms of lactic acid, 0.1 kilogram of citric acid, 0.1 kilogram of natrium citricum, 0.1 kilogram of flavoring apple essence, 328.2 kilograms of pure water;

2) pectin, locust bean gum, gelatin and propylene glycol alginate and white granulated sugar are mixed, be heated in 60 ℃ the milk, insulation dissolving 20 minutes, other gets 200 kilograms pure water and is warmed up to 55 ℃, add whole milk powder and albumen powder, oligoisomaltose (IMO), dissolved 15 minutes, more than both mix. Be cooled to 25 ℃ through cold drawing, stir after adding again fruit juice, essence constant volume.

3) being warmed up to 70 ℃, pressure is under the 20Mpa mixed liquor to be carried out homogeneous.

4) with step 3) feed liquid carry out 85 ℃, sterilization in 4 minutes.

5) be cooled to 40 ℃ after the sterilization and add bacterial classification, fermented 6 hours.

6) feed liquid that ferments is temporarily stored in the surge tank, to add in the material-compound tank with acidity regulator (lactic acid, natrium citricum and the citric acid) spray that normal temperature pure water (20 ℃) fully dissolves at last, continue in the spray process to stir, must not be interrupted, stop. Add flavoring apple essence, stir, 70 ℃, carried out sterilization, and then carried out sterile filling in 15 minutes.

This sour milk contains beneficial bacteria factor, and (oligoisomaltose (IMO), detection method is the same. ), can improve intestinal environment, promote the growth of beneficial bacterium. In this sour milk the metabolite that produces in the sweat is arranged also simultaneously, be conducive to absorption of human body. In addition, also add upper Juice, contain a large amount of vitamin and dietary fiber in it, make the nutrition of this sour milk abundanter.

The preparation of embodiment 3, beneficial bacteria factor long-acting sour milk

Raw material:

Milk: protein: 2.95%, fat: 3.0%, non-fat solid: 8.5%. White granulated sugar: meet country-level standard.

Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong Baolingbao Biotechnology Co., Ltd., model: Y001.

Gellan gum: the new hundred border industry and trade Co., Ltds that benefit the nation, model: U405; Xanthans: the new hundred border industry and trade Co., Ltds that benefit the nation, model: I405; Konjac glucomannan: port collection Group Co.,Ltd, model: O405; Dietary fiber: water insoluble dietary fiber 〉=60%. The new hundred border industry and trade Co., Ltds that benefit the nation, model: P405; Mango essence: laugh at and see well spices Co., Ltd, model: A498; Strawberry fruit grain: flesh-content 〉=55.0%; The fruit particle size is 2-5mm; The fruit plastochondria is pH3.6; Pol 20BX.

Pure water meets national standard.

Be prepared as follows long-acting sour milk:

1) by following composition of raw materials feeding (in 1 ton): 800.0 kilograms in milk, leavening 0.5 * 10⁸CFU/100g, 90.0 kilograms of white granulated sugars, 25 kilograms of oligoisomaltoses (IMO), 0.15 kilogram of gellan gum, 2.0 kilograms of xanthans, 1.0 kilograms of konjac glucomannans, 1.0 kilograms in mango essence, 5.0 kilograms of dietary fibers, 5.0 kilograms of fruit grains, 70.85 kilograms of pure water;

2) gellan gum, xanthans, konjac glucomannan and white granulated sugar are mixed, be heated in 65 ℃ the milk, insulation dissolving 20 minutes is cooled to 28 ℃ through cold drawing, adding fills in the material-compound tank of oligoisomaltose (IMO), dietary fiber again, fully mixes.

3) being warmed up to 55 ℃, pressure is under the 17Mpa mixed liquor to be carried out homogeneous.

4) with step 3) feed liquid carry out sterilization in 109 ℃, 8 minutes.

5) be cooled to 38 ℃ after the sterilization and add bacterial classification, fermented 6.6 hours.

6) feed liquid adding fruit grain and the mango essence that ferments is carried out 70 ℃, 20 minutes sterilizations, then sterile fillings.

This sour milk contains beneficial bacteria factor (oligoisomaltose (IMO), the method for detection is the same), can improve intestinal environment, promotes the growth of beneficial bacterium. In this sour milk the metabolite that produces in the sweat is arranged also simultaneously, be conducive to absorption of human body. In addition, also add upper fruit grain, contain a large amount of vitamin and dietary fiber in it, make the nutrition of this sour milk abundanter.

The preparation of embodiment 4, beneficial bacteria factor long-acting sour milk

Raw material:

Milk: protein: 3.25%, fat: 3.08%, non-fat solid: 8.56%. White granulated sugar: meet country-level standard.

Oligoisomaltose (IMO): IMO 〉=50%, available from Shandong Baolingbao Biotechnology Co., Ltd., model: S205 is for liquid.

Calcium lactate: meet national standard.

Pulp: strawberry pulp, pulp system pH 3.7, pol 35BX.

Acesulfame potassium: commerce and trade Co., Ltd of the excellent power in Beijing section, model: D452; Aspartame: commerce and trade Co., Ltd of the excellent power in Beijing section, model: D584; Gellan gum: Chengdu Hua Tong Food Co., Ltd, model: G648; Carragheen: Chengdu Hua Tong Food Co., Ltd, model: H648; Single stearic fat glyceride: the Inner Mongol hundred million sharp Bioisystech Co., Ltd, model: J456; Strawberry essence: Yinxiang Biological Engineering Co., Ltd., Zhejiang Prov, L684; Pure water all meets national standard.

Be prepared as follows long-acting sour milk:

1) by following composition of raw materials feeding (in 1 ton): milk: 893.5 kilograms, leavening 0.8 * 10⁸CFU/100g, white granulated sugar: 60.0 kilograms, acesulfame potassium: 0.06 kilogram, Aspartame: 0.004 kilogram, oligoisomaltose (IMO): 30.0 kilograms, gellan gum: 0.5 kilogram, sodium carboxymethylcellulose: 2.5 kilograms, 1.0 kilograms of carragheens, 0.8 kilogram of single stearic fat glyceride, calcium lactate: 1.0 kilograms, pulp: 10 kilograms, 0.75 kilogram of strawberry essence.

2) gellan gum, sodium carboxymethylcellulose, carragheen, single stearic fat glyceride, acesulfame potassium, Aspartame and white granulated sugar are mixed, be heated in 60 ℃ the milk, insulation dissolving 18 minutes, be cooled to 25 ℃ through cold drawing, adding fills in the material-compound tank of oligoisomaltose (IMO), pulp, calcium lactate again, fully mixes.

3) with step 2) feed liquid carry out sterilization in 77 ℃, 6 minutes.

4) be cooled to 42 ℃ after the sterilization and add bacterial classification, fermented 5 hours.

5) fermentation adding essence stirs, and carries out sterilization in 68 ℃, 25 minutes, sterile filling.

This sour milk contains beneficial bacteria factor (oligoisomaltose (IMO), the method for detection is the same), can improve intestinal environment, promotes the growth of beneficial bacterium. In this sour milk the metabolite that produces in the sweat is arranged also simultaneously, be conducive to absorption of human body. In addition, also add upper jam, contain a large amount of vitamin and dietary fiber in it, make the nutrition of this sour milk abundanter.

The zoopery of embodiment 5, oligoisomaltose (IMO) regulating intestinal canal flora function

Given the test agent: oligoisomaltose (IMO), available from the precious company of Shandong bowling, purity 50% is white powder. Preserve shady and cool, dry, ventilation.

Animal used as test: select the 18-22g available from Institute of Experimental Animals, Chinese Academy of Medical Sciences (credit number: SCXK-(capital) 2004-0001), 48 of the healthy cleaning level of BALB/C male mices are divided into four groups, 12 every group.

Dosage: the recommended amounts of oligoisomaltose (IMO) is adult (pressing the 60kg batheroom scale) 10g every day, 20g and 30g. According to three RDs 10 times arrange mouse experiment dosage respectively, and namely every daily dose is respectively 1.67g/kgBW (low dose group), 3.33g/kgBW (middle dosage group), 5.00g/kgBW (high dose group). Tested material water (sterilizing) preparation, per os gives the mouse tested material once a day, and gavage was tested indices after 14 days continuously. The mouse stomach volume is that the 0.10mL/10g mouse is heavy. Establish simultaneously blank group (0g/kgBW), water (sterilizing) replaces tested material, and every day, the gavage volume was identical with each tested material group.

Before giving given the test agent, the aseptic stool in mice number got is put in the aseptic vessel, uses the assay balance weighing, record weight. Then, in clean bench, sterile working adds dilution (redistilled water), is diluted to 10^-2, the mixing that fully vibrates is according to 10 times of serial dilutions to 10^-8, to measure bacterium for every kind and select suitable dilution factor, inoculation is dull and stereotyped, detects the Microflora of five kinds of typical bacterium of enteron aisle (Bifidobacterium, lactobacillus, enterococcus, enterobacteria, C.perfringens). Culture medium, culture ﹠ identification method are as shown in table 1, then calculate the wet clump count (cfu/g) in just of every gram, except C.perfringens, all take the logarithm and carry out statistical disposition. Give the last time afterwards 24h of given the test agent, again detect the Microflora of five kinds of typical bacterium of above-mentioned enteron aisle, method step is the same.

Data are processed: with SPSS software the initial data of each experimental group is carried out data and process, adopt the program of variance analysis to carry out first homogeneity test of variance, variance is neat, calculates the F value, F value＜F_0.05, conclusion: each organizes no significant difference between mean; F value 〉=F_0.05, P≤0.05 is added up with the in twos comparative approach of mean between a plurality of experimental group and control group; The data of Non-Gaussian Distribution or heterogeneity of variance are carried out suitable variable conversion, after satisfying the neat requirement of normal state or variance, add up with the data after the conversion; If still for to reach normal state or the neat purpose of variance, use rank test instead and add up after the variable conversion.

The result judges: last, criterion according to " health food check and assessment technique standard " (version in 2003), the number change situation of Bifidobacterium, lactobacillus, enterococcus, enterobacteria, C.perfringens between comparative experiments front and back self and group, experimental group experiment front and back self comparing difference has conspicuousness, or comparing difference has conspicuousness and experimental group experiment front and back self comparing difference that conspicuousness is arranged between the rear experimental group of experiment and control group group, meet with the next item down, can judge that this group given the test agent results of animal is positive: Bifidobacterium and/or lactobacillus obviously increase in (1) ight soil, C.perfringens reduces or does not increase, and enterobacteria, enterococcus are without significant change. (2) Bifidobacterium and/or lactobacillus obviously increase in the ight soil, and C.perfringens reduces or do not increase, and enterobacteria and/or enterococcus obviously increase, but the amplitude that increases is lower than the amplitude that Bifidobacterium/lactobacillus increases.

Oligoisomaltose (IMO) is as shown in table 3 on the testing result of Mouse Weight impact, body weight compares in each dosage group and 0g/kgBW group after the initial body weight of mouse and the experiment, there are no significant for difference (P＞0.05), shows that oligoisomaltose (IMO) has no adverse effects to the body weight of mouse.

The testing result of enterobacteria Microflora is as shown in table 4 in the mouse intestinal of experiment front and back, give between front each the dosage group of tested material and 0g/kgBW group and compare, the quantity of enterobacteria is all without significant difference (P＞0.05), and compare for tested material after 14 days with the 0g/kgBW group, 5.00g/kgBW the quantity of group enterobacteria reduces, there were significant differences (P＜0.05). Each dosage group self enterobacteria quantity there are no significant difference (P＞0.05) relatively before and after the experiment.

The testing result of enterococcus Microflora is as shown in table 5 in the mouse intestinal of experiment front and back, give between tested front each dosage group and 0g/kgBW group and compare, enterococcal quantity there are no significant difference (P＞0.05), compare for tested material after 14 days with the 0g/kgBW group, reduce 5.00g/kgBW organize enterococcal quantity, there were significant differences (P＜0.05). Each dosage group self enterococcus there are no significant difference (P＞0.05) relatively before and after the experiment.

The testing result of lactobacillus Microflora is as shown in table 6 in the mouse intestinal of experiment front and back, gives between forward and backward each the dosage group of tested material and 0g/kgBW group and compares the quantity there was no significant difference of lactobacillus (P＞0.05). Give forward and backward each dosage group of tested material self relatively, the quantity of lactobacillus there are no significant difference (P＞0.05).

Before and after the experiment in the mouse intestinal testing result of C.perfringens Microflora as shown in table 7, give between tested forward and backward each dosage group and 0g/kgBW group and compare, the quantity of C.perfringens there are no significant difference (P＞0.05). Respectively organize self relatively before and after giving tested material, the quantity of C.perfringens is all without significant difference (P＞0.05).

Before and after the experiment in the mouse intestinal testing result of Bifidobacterium Microflora shown in table 8 and table 9, by table 8 as can be known, give between tested forward and backward each dosage group and 0g/kgBW group and compare, the quantity of Bifidobacterium there are no significant difference (P＞0.05). Before and after giving tested material 0g/kgBW, 1.67g/kgBW group through self relatively, the quantity of Bifidobacterium there are no significant difference (P＞0.05); 3.33g/kgBW organize self relatively, the quantity of Bifidobacterium increases, and significant difference (P＜0.05) is arranged; 5.00g/kgBW experimental group self relatively, the quantity of Bifidobacterium increases, and significant difference (P＜0.01) is arranged. As shown in Table 9, give comparison between tested forward and backward each dosage group and 0g/kgBW group, the quantity of Bifidobacterium there are no significant difference (P＞0.05). The 3.33g/kgBW group self compares before and after giving tested material, and the quantity of Bifidobacterium increases by 1.4 times; 5.00g/kgBW organize self relatively, the quantity of Bifidobacterium increases by 2.0 times.

In sum, per os gives mouse oligoisomaltose (IMO) 14 days front and back, and the 3.33g/kgBW group self compares, and the quantity of Bifidobacterium increases by 1.4 times in the mouse intestines, and significant difference (P＜0.05) is arranged; 5.00g/kgBW organize self relatively, the quantity of Bifidobacterium increases by 2.0 times, and significant difference (P＜0.01) is arranged. Per os gave mouse oligoisomaltose (IMO) after 14 days, compared with the 0g/kgBW group, and 5.00g/kgBW group enterobacteria quantity reduces (P＜0.05); Enterococcus quantity significantly reduces (P＜0.05). Give before and after the oligoisomaltose (IMO), each organizes mouse C.perfringens quantity all without marked change. Oligoisomaltose (IMO) has no adverse effects to Mouse Weight.

Above-mentioned experimental result shows that oligoisomaltose (IMO) regulating intestinal canal flora contractile studies is positive, proves that oligoisomaltose (IMO) can the had significant proliferation intestinal bifidobacteria.

Table 2 gut flora check culture medium, culture ﹠ identification method

The bacterium name	Culture medium	Condition of culture	Authentication method
The bacterium name	Culture medium	Condition of culture	Authentication method	Enterobacteria	Eosin methylene blue agar (Qingdao Hai Bo Bioisystech Co., Ltd)	36±1℃，24h	Counting lactose fermenters, dyeing microscopic examination are G^-All bacterium colonies of bacillus
Enterococcus	Sodium azide-crystal violet-aesculin agar (Qingdao Hai Bo Bioisystech Co., Ltd)	36±1℃，48h	It is G that counting has obvious brown circle, dyeing microscopic examination⁺All bacterium colonies of coccus	Enterobacteria		36±1℃，24h
Enterococcus		36±1℃，48h		Lactobacillus	Lactobacillus selective medium (Qingdao Hai Bo Bioisystech Co., Ltd)	36±1℃，48h	According to GB/T4789.34-2003
Bifidobacterium	Bifidobacterium selective medium (Qingdao Hai Bo Bioisystech Co., Ltd)	Anaerobism is cultivated 36 ± 1 ℃, 48h	G ⁺Sporeless bacterium, the negative APICH50 of catalase	Lactobacillus		36±1℃，48h	According to GB/T4789.34-2003
Bifidobacterium		Anaerobism is cultivated 36 ± 1 ℃, 48h	G ⁺Sporeless bacterium, the negative APICH50 of catalase	C.perfringens	C.perfringens selective medium (Qingdao Hai Bo Bioisystech Co., Ltd)	Anaerobism is cultivated 36 ± 1 ℃, 24h	Count all have fluorescence under ultraviolet light black bacterium colony

Respectively organize the body weight (x ± SD) of mouse before and after table 3 experiment

Group	Number of animals (only)	Body weight (g) before the experiment	With 0g/kgBW group P value relatively	Body weight (g) after the experiment	With 0g/kgBW group P value relatively
Group	Number of animals (only)	Body weight (g) before the experiment	With 0g/kgBW group P value relatively	Body weight (g) after the experiment	With 0g/kgBW group P value relatively	0g/kgBW	12	21.4±0.9	……	26.6±1.2	……
1.67g/kgBW	12	21.1±1.3	0.895	25.5±1.7	0.156	0g/kgBW	12	21.4±0.9	……	26.6±1.2	……
1.67g/kgBW	12	21.1±1.3	0.895	25.5±1.7	0.156	3.33g/kgBW	12	21.4±1.1	1.000	26.3±1.8	0.890
5.00g/kgBW	12	21.4±0.8	1.000	25.4±1.2	0.137	3.33g/kgBW	12	21.4±1.1	1.000	26.3±1.8	0.890

Testing result (the log of enterobacteria Microflora in table 4 mouse intestinal₁₀cfu/g， x±SD)

Group	Number of animals (only)	Give tested material	With 0g/kgBW group P value relatively	After giving tested material	With 0g/kgBW group P value relatively	This group is to the P value before and after the tested material
Group	Number of animals (only)	Give tested material	With 0g/kgBW group P value relatively	After giving tested material	With 0g/kgBW group P value relatively		0g/kgBW	12	5.97±0.27	……	6.32±0.4	……	0.062
1.67g/kgBW	12	6.14±0.31	0.338	6.12±0.31	0.271	0.751	0g/kgBW	12	5.97±0.27	……	6.32±0.4	……	0.062
1.67g/kgBW	12	6.14±0.31	0.338	6.12±0.31	0.271	0.751	3.33g/kgBW	12	6.09±0.24	0.635	6.04±0.27	0.087	0.587
5.00g/kgBW	12	6.07±0.33	0.763	5.96±0.23 ^a	0.021	0.207	3.33g/kgBW	12	6.09±0.24	0.635	6.04±0.27	0.087	0.587

A: relatively there were significant differences with the 0g/kgBW group

Enterococcus Microflora testing result (log in table 5 mouse intestinal₁₀cfu/g， x±SD)

Group	Number of animals (only)	Give tested material	With 0g/kgBW group P value relatively	After giving tested material	With 0g/kgBW group P value relatively	This group is to the P value before and after the tested material
Group	Number of animals (only)	Give tested material	With 0g/kgBW group P value relatively	After giving tested material	With 0g/kgBW group P value relatively		0g/kgBW	12	6.98±0.30	……	7.29±0.61	……	0.068
1.67g/kgBW	12	7.04±0.38	0.974	7.11±0.39	0.677	0.546	0g/kgBW	12	6.98±0.30	……	7.29±0.61	……	0.068
1.67g/kgBW	12	7.04±0.38	0.974	7.11±0.39	0.677	0.546	3.33g/kgBW	12	6.82±0.53	0.669	6.84±0.47	0.065	0.828
5.00g/kgBW	12	8.92±0.50	0.971	6.74±0.40 ^a	0.019	0.292	3.33g/kgBW	12	6.82±0.53	0.669	6.84±0.47	0.065	0.828

A: relatively there were significant differences with the 0g/kgBW group

Table 6 mouse lactobacillus Microflora testing result (log₁₀cfu/g， x±SD)

Group	Number of animals (only)	Give tested material	With 0g/kgBW group P value relatively	After giving tested material	With 0g/kgBW group P value relatively	This group is to the P value before and after the tested material
Group	Number of animals (only)	Give tested material	With 0g/kgBW group P value relatively	After giving tested material	With 0g/kgBW group P value relatively		0g/kgBW	12	8.30±0.29	……	8.39±0.54	……	0.559
1.67g/kgBW	12	8.31±0.19	1.000	8.41±0.39	0.999	0.313	0g/kgBW	12	8.30±0.29	……	8.39±0.54	……	0.559
1.67g/kgBW	12	8.31±0.19	1.000	8.41±0.39	0.999	0.313	3.33g/kgBW	12	8.29±0.25	1.000	8.42±0.25	0.998	0.128
5.00g/kgBW	12	8.28±0.50	0.998	8.56±0.30	0.605	0.120	3.33g/kgBW	12	8.29±0.25	1.000	8.42±0.25	0.998	0.128

Table 7 mouse C.perfringens Microflora testing result (cfu/g, x ± SD)

Group	Number of animals (only)	Before giving tested material	With 0g/kgBW group P value relatively	After giving tested material	With 0g/kgBW group P value relatively	This group is to the P value before and after the tested material
Group	Number of animals (only)	Before giving tested material	With 0g/kgBW group P value relatively	After giving tested material	With 0g/kgBW group P value relatively		0g/kgBW	12	17±25	……	21±26	……	0.723
1.67g/kgBW	12	17±33	1.000	21±26	1.000	0.674	0g/kgBW	12	17±25	……	21±26	……	0.723
1.67g/kgBW	12	17±33	1.000	21±26	1.000	0.674	3.33g/kgBW	12	17±23	0.970	12±23	0.736	1.000
5.00g/kgBW	12	17±33	1.000	12±23	0.736	0.586	3.33g/kgBW	12	17±23	0.970	12±23	0.736	1.000

Bifidobacterium Microflora testing result (log in table 8 mouse intestinal₁₀cfu/g， x±SD)

Group	Number of animals (only)	Give tested material	With 0g/kgBW group P value relatively	After giving tested material	With 0g/kgBW group P value relatively	This group is to the P value before and after the tested material
Group	Number of animals (only)	Give tested material	With 0g/kgBW group P value relatively	After giving tested material	With 0g/kgBW group P value relatively		0g/kgBW	12	8.60±0.23	……	8.77±0.36	……	0.165
1.67g/kgBW	12	8.59±0.36	1.000	8.81±0.36	0.984	0.062	0g/kgBW	12	8.60±0.23	……	8.77±0.36	……	0.165
1.67g/kgBW	12	8.59±0.36	1.000	8.81±0.36	0.984	0.062	3.33g/kgBW	12	8.55±0.30	0.970	8.88±0.40 ^b	0.805	0.040
5.00g/kgBW	12	8.65±0.36	0.956	9.11±0.35 ^b	0.069	0.001	3.33g/kgBW	12	8.55±0.30	0.970	8.88±0.40 ^b	0.805	0.040

B: relatively there were significant differences before to tested material with this group

Bifidobacterium Microflora testing result (* 10 in table 9 mouse intestinal⁸cfu/g， x±SD)

Group

Number of animals (only)

Before giving tested material

After giving tested material

This group is to relatively increasing before and after the tested material

0g/kgBW	12	4.4±1.9	7.5±5.2	——
0g/kgBW	12	4.4±1.9	7.5±5.2	——	1.67g/kgBW	12	5.4±4.7	8.3±5.3	——
3.33g/kgBW	12	4.5±3.9	10.8±9.4	1.4 doubly	1.67g/kgBW	12	5.4±4.7	8.3±5.3	——
3.33g/kgBW	12	4.5±3.9	10.8±9.4	1.4 doubly	5.00g/kgBW	12	5.5±2.9	16.5±10.6	2.0 doubly

The human experimentation of embodiment 6, oligoisomaltose (IMO) regulating intestinal canal flora function

Tested crowd: through 60 of all normal adults of clinical health check-up index, the men and women half and half. 30 constipation persons by name among the experimenter.

The enteric bacteria cultural method is as follows:

Bifidobacterium: BBL agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, anaerobism was cultivated in 48-72 hour;

Lactobacillus: LBs agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, cultivated in 48 hours;

Enterobacteria: EMB agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, cultivated in 24-48 hour;

Enterococcus: Sodium azide-crystal violet-aesculin agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, cultivated in 24-48 hour;

Bacteroid: Bd agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, anaerobism was cultivated in 24 hours;

C.perfringens: TSC agar medium (Qingdao Hai Bo Bioisystech Co., Ltd), 37 ℃, anaerobism was cultivated in 24 hours.

Randomly draw above-mentioned 60 routine experimenters, be divided into 3 groups (basic, normal, high dosage groups), every group of 20 people, the men and women half and half. Before the test-meal given the test agent, the aseptic experimenter's ight soil of taking is put in the aseptic vessel, uses the assay balance weighing, record weight. Then, in clean bench, sterile working adds dilution (redistilled water), is diluted to 10^-2, the mixing that fully vibrates is according to 10 times of serial dilutions to 10^-8, to measure bacterium for every kind and select suitable dilution factor, inoculation is dull and stereotyped, checks above-mentioned six kinds of gut flora numbers (Bifidobacterium, lactobacillus, enterobacteria, enterococcus, bacteroid, C.perfringens). Then, basic, normal, high dosage group experimenter takes respectively oligoisomaltose (IMO) 5g, 10g, 15g every day, continuously 14d. Respectively at taking tested material 3d, 7d, 10d, 14d, and when withdrawing rear 3d, 7d, 14d, the aseptic experimenter's of taking ight soil detects and detects above-mentioned six kinds of gut flora numbers with same procedure. Observe, record the edible front and back of experimenter subjective symptoms.

Data processing method is identical with embodiment 5.

Take 5g tested material (low dose group) every day, take 14d, the intestinal microflora testing result is shown in table 10, and the enterobacteria of 5g/d experimental group, enterococcus, C.perfringens are taken forward and backward quantity without marked change (p＞0.05); Bacteroid after taking 10d and withdraw after 3d take before quantity increase, difference has conspicuousness (p＜0.05; Bifidobacterium 7d, 10d quantity after taking increase, and difference has conspicuousness (p＜0.05); Lactobacillus 7d quantity after withdrawing reduces, and difference has utmost point conspicuousness (p＜0.01).

Take tested material 10g (middle dosage group) every day, take 14d, the intestinal microflora testing result shown in table 11, the intestines liver bacterium of 10g/d experimental group, lactobacillus quantity no significant difference (p＞0.05); Enterococcus 14d quantity after withdrawing increases, and difference has conspicuousness (p＜0.05); Bacteroid after taking 10d and withdraw after 3d quantity increase, wherein, 10d difference tool utmost point conspicuousness (p＜0.01) after taking, 3d difference tool conspicuousness (p＜0.05) after withdrawing; C.perfringens 7d, 10d, 14d quantity after taking descend, 10d difference tool utmost point conspicuousness (p＜0.01) wherein, 7d, 14d difference tool conspicuousness (p＜0.05); Bifidobacterium after taking 7d, 10d and withdraw after 7d quantity increase, 10d difference tool utmost point conspicuousness (p＜0.01) after taking, after taking 7d and withdraw after 7d difference tool conspicuousness (p＜0.05).

Take tested material 15g (high dose group) every day, take 14d, the intestinal microflora testing result is shown in table 12, and the enterococcus of 15g/d experimental group, lactobacillus quantity are without significant change (p＞0.05); Enterobacteria after taking 14d and withdraw after 3d quantity reduce, difference has conspicuousness (p＜0.05); Bacteroid after 7d, 14d withdraw after taking 7d, 14d quantity reduce, after wherein taking 7d and withdraw after 14d difference utmost point conspicuousness (p＜0.01) is arranged, after taking 14d and withdraw after 7d difference conspicuousness (p＜0.05) is arranged; The pretty bacterium of perfringens is 7d and the decline of 10d quantity after taking, and wherein 10d difference has utmost point conspicuousness (p＜0.01), and 7d difference has conspicuousness (p＜0.05); Bifidobacterium 3d, 10d quantity after taking increase the equal tool utmost point of difference conspicuousness (p＜0.01)

In sum, take continuously 14d with the oligoisomaltose (IMO) of 5g/d, 10g/d, 15g/d dosage, Bifidobacterium is significantly increased. The 5g/d experimental group compares through self, and the quantity of Bifidobacterium increases by 10 times, and (P＜0.05), difference has conspicuousness; 10g/d experimental group and 15g/d experimental group compare through self, and the quantity of Bifidobacterium increases by 10 times, difference tool utmost point conspicuousness (P＜0.01). Above-mentioned experimental result shows that the bifidobacteria of each dosage group all is significantly improved, and proves that further oligoisomaltose (IMO) can obviously promote Bifidobacterium propagation in the enteron aisle.

Experimental session records main suit's symptom of experimenter every day, after the experimenter takes tested material-oligoisomaltose (IMO) as a result, the defecation frequency rule, the ight soil proterties is normal, defecation is unobstructed, diet, sleep and the state of mind all keep well, prove that oligoisomaltose (IMO) has no adverse effects to the people, takes safety.

Table 10 is taken oligoisomaltose human body intestinal canal flora observing results I (5g/d) (log₁₀CFU/g， x±S，

n＝10)

Bacterium	Enterobacteria	Enterococcus	Bacteroid	C.perfringens	Bifidobacterium	Lactobacillus
Bacterium	Enterobacteria	Enterococcus	Bacteroid	C.perfringens	Bifidobacterium	Lactobacillus	Before taking	7.11±1.38	5.95±1.32	8.05±2.28	1.79±0.93	8.00±1.81	8.11±1.04
Take rear 3d	7.53±0.97	5.59±1.32	8.06±1.95	1.63±0.81	8.29±1.76	8.32±0.75	Before taking	7.11±1.38	5.95±1.32	8.05±2.28	1.79±0.93	8.00±1.81	8.11±1.04
Take rear 3d	7.53±0.97	5.59±1.32	8.06±1.95	1.63±0.81	8.29±1.76	8.32±0.75	7d	7.10±1.13	5.72±1.67	8.80±2.18	1.81±1.00	9.32±0.44*	7.97±0.80
10d	7.32±0.85	5.80±1.48	9.74±0.82*	1.67±1.00	9.50±0.63*	7.57±1.28	7d	7.10±1.13	5.72±1.67	8.80±2.18	1.81±1.00	9.32±0.44*	7.97±0.80
10d	7.32±0.85	5.80±1.48	9.74±0.82*	1.67±1.00	9.50±0.63*	7.57±1.28	14d	7.63±0.99	6.24±1.03	9.42±0.52	1.42±1.40	8.86±0.99	7.99±0.62
Withdraw rear 3d	7.42±0.52	6.27±1.74	9.71±0.27*	2.53±1.22	8.79±0.71	7.95±0.63	14d	7.63±0.99	6.24±1.03	9.42±0.52	1.42±1.40	8.86±0.99	7.99±0.62
Withdraw rear 3d	7.42±0.52	6.27±1.74	9.71±0.27*	2.53±1.22	8.79±0.71	7.95±0.63	7d	6.57±0.86	5.84±1.06	9.46±0.66	2.32±1.67	8.60±0.76	7.68±0.69
14d	7.10±0.92	6.17±1.25	7.37±2.59	2.49±1.44	8.59±0.57	7.31±1.59	7d	6.57±0.86	5.84±1.06	9.46±0.66	2.32±1.67	8.60±0.76	7.68±0.69

* expression: take after the tested material and take before the tested material relatively p＜0.05, * * represents: take after the tested material and take before the tested material relatively p＜0.01.

Table 11 is taken oligoisomaltose human body intestinal canal flora observing results II (10g/d) (log₁₀CFU/g， x±S，

n＝10)

Bacterium	Enterobacteria	Enterococcus	Bacteroid	C.perfringens	Bifidobacterium	Lactobacillus
Bacterium	Enterobacteria	Enterococcus	Bacteroid	C.perfringens	Bifidobacterium	Lactobacillus	Before taking	6.73±1.37	5.21±1.11	8.03±2.18	2.00±0.99	7.75±1.58	7.24±1.36
Take rear 3d	7.36±0.99	6.00±1.29	8.20±2.30	2.25±1.02	8.57±0.58	7.76±0.82	Before taking	6.73±1.37	5.21±1.11	8.03±2.18	2.00±0.99	7.75±1.58	7.24±1.36
Take rear 3d	7.36±0.99	6.00±1.29	8.20±2.30	2.25±1.02	8.57±0.58	7.76±0.82	7d	6.64±0.79	4.82±2.03	8.48±2.32	1.25±0.71*	8.93±0.44*	7.90±1.09
10d	6.77±0.83	5.28±1.10	9.96±0.31**	1.02±0.02**	9.26±0.36**	7.41±0.96	7d	6.64±0.79	4.82±2.03	8.48±2.32	1.25±0.71*	8.93±0.44*	7.90±1.09
10d	6.77±0.83	5.28±1.10	9.96±0.31**	1.02±0.02**	9.26±0.36**	7.41±0.96	14d	6.72±1.01	4.85±1.62	8.88±2.25	1.18±0.49*	8.62±0.74	7.41±0.96
Withdraw rear 3d	7.03±0.55	5.59±0.89	9.66±0.36*	2.41±1.13	8.61±0.43	7.36±0.72	14d	6.72±1.01	4.85±1.62	8.88±2.25	1.18±0.49*	8.62±0.74	7.41±0.96
Withdraw rear 3d	7.03±0.55	5.59±0.89	9.66±0.36*	2.41±1.13	8.61±0.43	7.36±0.72	7d	7.20±0.39	5.88±1.14	9.38±1.18	2.52±1.21	8.99±0.44*	7.72±0.60
14d	7.09±0.22	6.51±1.20*	8.47±2.00	1.63±0.97	8.73±0.61	7.61±0.73	7d	7.20±0.39	5.88±1.14	9.38±1.18	2.52±1.21	8.99±0.44*	7.72±0.60

Table 12 is taken oligoisomaltose human body intestinal canal flora observing results III (15g/d) (log₁₀CFU/g， x±S，

n＝10)

Bacterium	Enterobacteria	Enterococcus	Bacteroid	C.perfringens	Bifidobacterium	Lactobacillus
Bacterium	Enterobacteria	Enterococcus	Bacteroid	C.perfringens	Bifidobacterium	Lactobacillus	Before taking	7.72±0.67	5.38±0.64	9.91±0.24	2.59±1.41	8.69±0.46	7.68±1.48
Take rear 3d	7.76±1.00	7.01±2.07	9.95±0.26	2.65±1.47	9.27±0.25**	8.24±1.69	Before taking	7.72±0.67	5.38±0.64	9.91±0.24	2.59±1.41	8.69±0.46	7.68±1.48
Take rear 3d	7.76±1.00	7.01±2.07	9.95±0.26	2.65±1.47	9.27±0.25**	8.24±1.69	7d	7.18±0.93	5.38±1.47	7.87±2.07**	1.55±0.82*	9.06±0.66	7.78±0.92

10d	7.56±0.86	6.41±1.38	9.88±0.40	1.24±0.69**	9.67±0.50**	8.21±0.86
10d	7.56±0.86	6.41±1.38	9.88±0.40	1.24±0.69**	9.67±0.50**	8.21±0.86	14d	7.18±0.59*	5.86±1.43	9.64±0.30*	1.88±1.14	8.88±0.52	7.91±0.34
Withdraw rear 3d	6.95±0.88*	5.60±1.56	9.10±1.93	2.08±1.31	8.92±0.20	7.58±0.98	14d	7.18±0.59*	5.86±1.43	9.64±0.30*	1.88±1.14	8.88±0.52	7.91±0.34
Withdraw rear 3d	6.95±0.88*	5.60±1.56	9.10±1.93	2.08±1.31	8.92±0.20	7.58±0.98	7d	7.42±0.71	6.28±1.62	9.60±0.46*	1.99±1.52	8.99±0.47	7.92±0.85
14d	7.20±0.43	6.51±1.84	8.33±1.92**	1.58±1.15	8.26±0.68	7.60±0.61	7d	7.42±0.71	6.28±1.62	9.60±0.46*	1.99±1.52	8.99±0.47	7.92±0.85

Embodiment 7 product system stable detection

Take the product of embodiment 1-4 as laboratory sample, under normal temperature (23-36 ℃) environmental condition, carry out static observation, the testing product stability of system.

The stability test of product system: take by weighing a certain amount of product, carry out centrifugally, directly record the amount (weight in wet base) of BSW by difference assay, calculate the ratio that BSW accounts for gross weight, thus reflection albumen precipitation and bleed situation; Observe simultaneously the amount of albumen precipitation and bleed, result such as table 13.

Table 13 product system stability observing record sheet

	Embodiment 1	Embodiment 2	Embodiment 3	Embodiment 4
	Embodiment 1	Embodiment 2	Embodiment 3	Embodiment 4	7 days	Product is without albumen precipitation and bleed, stable system.	Product is without albumen precipitation and bleed, stable system.	Product is without albumen precipitation and bleed, stable system.	Product is without albumen precipitation and bleed, stable system.
25 days	Product system is stable, and without bleed, sediment accounts for 0.2%	Product system is stable, and without bleed, sediment accounts for 0.16%	Product system is stable, and without bleed, sediment accounts for 0.15%	Product system is stable, and without bleed, sediment accounts for 0.22%	7 days
25 days					45 days	Slight sediment is arranged, account for 0.6% of gross weight, without bleed	Slight white depositions accounts for 0.63% of gross weight, without bleed	Slight precipitation is arranged, and sediment accounts for 0.70%, without bleed	A small amount of white depositions accounts for 0.75% of gross weight, without bleed
65 days	A small amount of sediment accounts for 1.1% of gross weight, without bleed	A small amount of sediment accounts for 1.4% of gross weight, without bleed,	A small amount of sediment 1.17% is without bleed	A small amount of sediment accounts for 1.35% of gross weight, without bleed,	45 days
65 days			A small amount of sediment 1.17% is without bleed		85 days	Certain sediment is arranged, account for 1.7% of gross weight, some bleeds are arranged	White depositions begins to increase, and accounts for 1.9% of gross weight, and some bleeds are arranged	A small amount of precipitation, sediment accounts for 2.0% of gross weight, and some bleeds are arranged	The adularescent sediment accounts for 1.95% of gross weight, and some bleeds are arranged
115 days	Sediment begins to increase, and accounts for 2.8% of gross weight, and some bleeds are arranged, and can accept	White depositions begins to increase, and accounts for 3.1% of gross weight, and some bleeds are arranged, and can accept	Sediment is more obvious, accounts for 3.2% of gross weight, and some bleeds are arranged, and can accept	White depositions increases, and accounts for 3.0% of gross weight, and some bleeds are arranged, and can accept	85 days
115 days					150 days	Sediment obviously occurs, and accounts for 4.1% of gross weight, and some bleeds are arranged, and can accept	White depositions is apparent in view, accounts for 4.3% of gross weight, and some bleeds are arranged, and can accept	The sediment showed increased accounts for 4.5% of gross weight, and some bleeds are arranged, and can accept	White depositions obviously increases, and accounts for 4.4% of gross weight, and some bleeds are arranged, and can accept

180 days

Sediment obviously occurs, and accounts for 4.1% of gross weight, and some bleeds are arranged, and can accept

White depositions is apparent in view, accounts for 4.5% of gross weight, and some bleeds are arranged, and can accept

The sediment showed increased accounts for 4.5% of gross weight, and some bleeds are arranged, and can accept

White depositions obviously increases, and accounts for 4.4% of gross weight, and some bleeds are arranged, and can accept

Claims

1, a kind of long-acting sour milk is characterized in that: it is made by the raw material of the component that contains leavening and following weight portion:

Milk 50-100,

Oligoisomaltose 0.01-20.00,

Stabilizing agent 0.2-8.0.

2, long-acting sour milk according to claim 1 is characterized in that: described milk is common cow's milk, comprises full-cream, degreasing and half skimmed milk; Or with the formulated recombined milk of other components of milk powder, cream, protein hydrolysate, whey powder or cow's milk;

The amount of described oligoisomaltose is take the oligoisomaltose of 50% purity as standard metering;

The content of described oligoisomaltose is for being the 0.01-15 weight portion.

3, long-acting sour milk according to claim 2 is characterized in that: the acidity regulator that also contains the 0.2-8.0 weight portion in the described raw material.

4, long-acting sour milk according to claim 3 is characterized in that: also contain Juice, jam, fruit-vegetable granules or the nut granule of 0-20 weight portion in the described raw material, or the trace element of 0.01-0.5 weight portion.

5, long-acting sour milk according to claim 4 is characterized in that: the flavoring essence and/or the white granulated sugar of 3.0-15.0 weight portion and syrup or the sweetener of 0-5 weight portion that also contain the 0.01-0.4 weight portion in the described raw material; Described sweetener is acesulfame-K and Aspartame, and described syrup is the one or more combination in fructose and the HFCS.

6, the described long-acting sour milk of arbitrary claim in 5 according to claim 1, it is characterized in that: the bacterial classification content of the leavening in the described raw material is 10⁶-10 ⁸The CFU/100g raw material.

7, long-acting sour milk according to claim 6 is characterized in that: described leavening is a kind of or its any combination in streptococcus thermophilus, streptococcus lactis, streptococcus cremoris, lactobacillus acidophilus, lactobacillus bulgaricus, missible oil streptococcus, bacterium, lemon leukonid, the bright string strain of pentose bacterium, diacetyl streptococcus lactis, thermophilic lactobacillus, Lactobacillus casei, Bifidobacterium, bifidobacterium bifidum, bifidobacterium longum, bifidobacterium infantis and the bifidobacterium breve that produces aromatic substance.

8, long-acting sour milk according to claim 7 is characterized in that: described stabilizing agent is emulsifying agent or thickener, or is combined by emulsifying agent and thickener, and wherein, the ratio of weight and number of emulsifying agent and thickener is 1: 1-60; Described emulsifying agent is a kind of or its any combination in sucrose fatty ester, fatty acid glyceride and the polyglycerol ester; Described thickener is a kind of or its any combination in sodium carboxymethylcellulose, carragheen, gelatin, distortion starch, albumen powder, D-glucose, microcrystalline cellulose, gellan gum, xanthans, propylene glycol alginate, konjac glucomannan, agar, locust bean gum and the pectin;

Described acidity regulator is a kind of or its any combination in lactic acid, citric acid, phosphoric acid, tartaric acid and malic acid and the salt thereof; Described flavoring essence is a kind of or its any combination in sour milk essence, milk flavour, orange essence, grape essence, peach essence, cocoanut flavour, strawberry essence, lychee flavor, flavoring apple essence, close melon essence, mango essence, passionflower essence, grapefruit essence, lemon extract, blueberry essence, pawpaw essence, flavoring banana essence, flavoring pineapple essence, tomato flavour, carrot essence, asparagus essence, spinach essence, apricot essence, pear essence, kiwi flavor, vanilla, flavoring rose essence, chocolate essence, walnut essence, wheat essence and the cherry essence.

9, long-acting sour milk according to claim 8, it is characterized in that: described fruit-vegetable granules is selected from the really combination of one or more in grain, celery particle, the operatic circle grain, cucumber particle, radish kind particle and the aloe gel particle of tomato particle, peach particle, coconut palm fruit granule, orange fruit grain, mango fruit grain, grape fruit, pawpaw fruit grain, pineapple sarcocarp grain, strawberry, described fruit-vegetable granules is selected granular garden stuff grain or the slurry class fruit and vegetable of diameter 1-20mm, or length is that 1-20mm, width are the fibrous fruit and vegetable of 1-10mm; Described Juice is selected from one or more the combination in cider, strawberry juice, blueberry juice, Chinese flowering quince juice, bananas juice, orange juice, pineapple juice, mango juice, tomato juice, carrot juice, asparagus juice, spinach juice, peach juice, apricot juice, pear juice, grape juice, asparagus juice, kiwi-fruit juice, Lychee juice, lemon juice and the Coconut Juice; Described jam is selected from one or more the combination in apple jam, strawberry jam, blueberry sauce, Chinese quince soy sauce, banana sauce, orange sauce, pineapple jam, mango chutney, catsup, carrot sauce, asparagus sauce, spinach sauce, peach butter, apricot sauce, pears sauce, grape sauce, aloe paste, kiwi-fruit jam, lichee sauce, jam with lemon and the coconut palm sauce; Described nut granule is selected one or more in peanut particle, fibert particle, walnut particle, sunflower seeds particle, almond particle, plum particle and the American pistachios particle, it is the spherical particle of 1-18mm that particle is selected diameter, perhaps length is 1-20mm, the particle of width 1-12mm;

Described trace element is selected from a kind of or its any combination in vitamin A, vitamin B complex, vitamin D, vitamin C, vitamin E, calcium lactate, taurine, folic acid, nicotinic acid, choline, ferrous sulfate, ironic citrate, zinc sulfate, zinc gluconate, sodium selenite, magnesium sulfate, magnesium gluconate and the l-cn.

10, a kind of method of producing long-acting sour milk claimed in claim 9 may further comprise the steps: