CN101056992A - 含有cpg岛的分离的核酸片段的应用 - Google Patents

含有cpg岛的分离的核酸片段的应用 Download PDF

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Publication number
CN101056992A
CN101056992A CNA2005800375162A CN200580037516A CN101056992A CN 101056992 A CN101056992 A CN 101056992A CN A2005800375162 A CNA2005800375162 A CN A2005800375162A CN 200580037516 A CN200580037516 A CN 200580037516A CN 101056992 A CN101056992 A CN 101056992A
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China
Prior art keywords
nucleic acid
cpg
cpg island
island
peptide
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CNA2005800375162A
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English (en)
Chinese (zh)
Inventor
阿德里安·彼德·伯德
罗伯特·斯科特·伊林沃思
黑勒·费尔科·乔根森
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University of Edinburgh
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University of Edinburgh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/809—Multifield plates or multicontainer arrays

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CNA2005800375162A 2004-10-29 2005-10-31 含有cpg岛的分离的核酸片段的应用 Pending CN101056992A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0423991.9A GB0423991D0 (en) 2004-10-29 2004-10-29 Applications of nucleic acid fragments
GB0423991.9 2004-10-29

Publications (1)

Publication Number Publication Date
CN101056992A true CN101056992A (zh) 2007-10-17

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CNA2005800375162A Pending CN101056992A (zh) 2004-10-29 2005-10-31 含有cpg岛的分离的核酸片段的应用

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US (1) US8105787B2 (enExample)
EP (1) EP1807533B1 (enExample)
JP (1) JP2008518596A (enExample)
CN (1) CN101056992A (enExample)
AU (1) AU2005298360A1 (enExample)
CA (1) CA2585831A1 (enExample)
GB (1) GB0423991D0 (enExample)
WO (1) WO2006046076A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102776270A (zh) * 2011-05-12 2012-11-14 中国科学院上海生命科学研究院 检测dna甲基化的方法和装置
CN111647643A (zh) * 2020-06-05 2020-09-11 南京邮电大学 一种dna特异性甲基化位点的制备及检测方法

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE335816T1 (de) 2002-09-18 2006-09-15 Sirs Lab Gmbh Verfahren zum nachweis prokaryontischer dna
ES2384571T3 (es) * 2004-03-05 2012-07-09 Sirs-Lab Gmbh Procedimiento para el enriquecimiento y/o la separación de ADN procariota mediante una proteína que se une específicamente a ADN que contiene motivos CpG no metilados
AU2005308918B2 (en) 2004-11-29 2012-09-27 Sequenom, Inc. Means and methods for detecting methylated DNA
JP5774805B2 (ja) * 2004-11-29 2015-09-09 セクエノム，インコーポレイティド メチル化ｄｎａを検出する方法、及びキット
WO2012058634A2 (en) * 2010-10-28 2012-05-03 Salk Institute For Biological Studies Epigenomic induced pluripotent stem cell signatures
US9461716B2 (en) * 2015-03-02 2016-10-04 Intel IP Corporation Near field communications (NFC) modulation feedback apparatus for tuned antenna configurations
WO2020216966A1 (en) * 2019-04-26 2020-10-29 Thermo Fisher Scientific Baltics Uab Isolated nucleic acid binding domains
NL2031337B1 (en) * 2022-03-18 2023-09-29 Univ Twente Method for separating high-methylated DNA and low-methylated DNA

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6605432B1 (en) 1999-02-05 2003-08-12 Curators Of The University Of Missouri High-throughput methods for detecting DNA methylation

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102776270A (zh) * 2011-05-12 2012-11-14 中国科学院上海生命科学研究院 检测dna甲基化的方法和装置
CN111647643A (zh) * 2020-06-05 2020-09-11 南京邮电大学 一种dna特异性甲基化位点的制备及检测方法

Also Published As

Publication number Publication date
EP1807533A2 (en) 2007-07-18
WO2006046076A3 (en) 2006-09-08
EP1807533B1 (en) 2015-07-01
AU2005298360A1 (en) 2006-05-04
WO2006046076A2 (en) 2006-05-04
US20080076671A1 (en) 2008-03-27
JP2008518596A (ja) 2008-06-05
US8105787B2 (en) 2012-01-31
CA2585831A1 (en) 2006-05-04
GB0423991D0 (en) 2004-12-01

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Open date: 20071017