CN101033449A - Optical bioreactor for spacing regenerative oxygen - Google Patents

Optical bioreactor for spacing regenerative oxygen Download PDF

Info

Publication number
CN101033449A
CN101033449A CNA2006101620914A CN200610162091A CN101033449A CN 101033449 A CN101033449 A CN 101033449A CN A2006101620914 A CNA2006101620914 A CN A2006101620914A CN 200610162091 A CN200610162091 A CN 200610162091A CN 101033449 A CN101033449 A CN 101033449A
Authority
CN
China
Prior art keywords
bioreactor
stirring
gas
illumination
reactor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA2006101620914A
Other languages
Chinese (zh)
Inventor
刘红
杨晨亮
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beihang University
Original Assignee
Beihang University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beihang University filed Critical Beihang University
Priority to CNA2006101620914A priority Critical patent/CN101033449A/en
Publication of CN101033449A publication Critical patent/CN101033449A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

This invention discloses a photobiological reactor including a main body of the reactor, an illumination system, gas supply system, a nutrient fluid injection system, a milling system, a temperature test and control system and a DO, pH test device, which utilizes a time controller to add a certain volume of nutrient fluid to the reactor definitely and penetrates CO2 to be distributed uniformly in the reactor by the milling system to generate O2 by the photosynthesis of micro-algas and generate biomass at the same time to makeup O2 and food to spacial cabins.

Description

The bioreactor that is used for spacing regenerative oxygen
One, technical field
The present invention relates to a kind of bioreactor of regeneration of oxygen, be mainly used in CO in space station, spacelab, the permanent base at space (as the moon, Mars base) 2Purification and O 2Regeneration.
Two, background technology
Life-support system are manned space flight key techniques, CO 2Purification and O 2Regeneration be key in this key.Little algae can utilize luminous energy to carry out photosynthesis regeneration O 2Purify CO 2Bioreactor is to influence micro algae growth and regeneration O as micro algae culturing device 2The key factor of speed.Design an algae culture bioreactor that is suitable for long-term interstellar flight, have crucial meaning for the safety and reliability that reduce space trucking costs, raising closed system.
The design of bioreactor will be satisfied: simple in structure, be convenient to the assembling and the operation of space test, and efficiency of light energy utilization height maximally utilises energy, reduces the energy consumption of following long-term flight, is fit to the continuous high-density culture of little algae.
One of key of bioreactor design is the illumination surface to volume ratio that will provide high, its objective is that raising transmittance and frustule are to the efficiency of light energy utilization; Another key of design is to select suitable circulation device for use, reduces the injury of shearing force to frustule; CO in addition 2Dissolving and when gas-liquid mass transfer, photosynthetic oxygen evolution dissolved oxygen accumulate selection with gas transfer efficiency such as discharge in time and light source quality and form, all will great influence be arranged to the little algae of bioreactor high-efficient culture.
In life-support system, be used at present airtight bioreactor that little algae cultivates mainly contain air lift type (MELISSA, ESA) and board-like (BIOS-3, Russia).Airlift photobioreactor has been avoided the injury of shearing force, but illuminating area and volume ratio are less, how to increase illuminating area and volume ratio, and does not influence other performances, is the subject matter that needs solution.Board-like bioreactor is to assemble the simplest class reactor, be convenient to realize the cultivation of the little algae of high-density, but mass transfer and heat transfer property is relatively poor.
Bioreactor will be obtained the high-density high-efficient culture, and the influence factor of most critical is the continuous supply of luminous energy conversion, gaseous interchange, nutrition source and biological limits factor etc.There are many drawbacks in present widely used light source as sunlight, metal halid lamp, cold incandescent light etc., in order to improve conversion of solar energy, selects high light intensity, narrow spectrum, energy-conservation, reliable light source to have great importance.
Three, technology contents
Technical problem to be solved by this invention is, provide a kind of simple in structure, the efficiency of light energy utilization is high, the culture density height is easy to control, can be used for the O in cabins such as space base 2Regenerating unit.
For achieving the above object, the technical scheme of taking is:
The present invention mainly comprises bioreactor main body 4, illumination system, airing system, nutritive medium loading system, stirring system, temperature measuring and Controlling System, DO, pH determinator.
Described bioreactor main body 4 employing transmittances material are preferably made, and are divided into area of illumination 402 and gas-liquid separation and DATA REASONING district 404, and the area of illumination aspect ratio is at 2.5-5, and illuminating area and volume of culture ratio (S/V) are at 20-50m -1Reactor head is provided with venting port 601, and from then on gas discharged and entered gas treating system 15.Gas-liquid separation and 4 middle parts, DATA REASONING district are provided with leakage fluid dram 405, and the algae water mixed liquid enters algae water separator 16 from here.The area of illumination mid-way is provided with thief hole 403, and the bottom is provided with fluid inlet 401.
Described stirring system is made up of gas distributor 1, stirring pump controller 13, stirring pump 505, reducing 504, sealing bearing 503, contiguous block 502, U type stirring-head 501 (3).U type stirring-head 501 usefulness Stainless Steel Wires are made core, make the U type behind the parcel plastics tubing and be fixed in contiguous block 502, link to each other with stirring pump 505 main shafts again, stirring velocity is regulated by stirring pump controller 13,, stirring velocity can reach 200r/min, can be used for the thin Spirulina of culturing cell wall etc., can not cause the fragmentation of alga cells, realize CO simultaneously 2With the thorough mixing of algae liquid, and guarantee fully contacting of frustule and light source, reach making full use of of light source; Gas enters reactor by gas distributor 1, and bubble rises and realizes stirring air flow 20L/hL.
Described illumination system is made up of light source 3 and light source controller 2, adopts the higher LED of present light efficiency as light source, and emmission spectrum is narrow but can cover algae and carry out photosynthetic spectral range; Be arranged in around the reactor by dot matrix, utilize light source controller 2 control brightness values.
Described nutritive medium loading system is made up of time controller 18, surge pump (recycle pump 17 and fluid filling pump 19), nutritive medium storage tank 20.Circulation and the nutritive medium of working order realization algae water mixed liquid between thief hole 403 and fluid inlet 401 by time controller 18 Control Circulation pumps 17 and fluid filling pump 19 annotated to the pulsed of reactor from storage tank, prevents algae adhering at circulation loop simultaneously.Liquid volume added can be controlled at 0.001-0.01L/L as requested, and the timed interval can be controlled at 1-12 hour.
Described temperature controlling system is made up of temp probe 22, temperature controller 14 and blower fan 23.Blower fan 23 is started working freezing air is blown to light source 3 and reactor surface when temperature of reactor surpasses certain value, take away more than heat, realize temperature controlling.
Described airing system is by CO 2Gas cylinder 8, air compressor pump 7, gas meter 9, gas mixer 10 and strainer 21 are formed.Pass through CO 2Gas cylinder 8 and air compressor pump 7 utilize the Atmospheric composition under meter 9 virtual space cabins, control CO 2Concentration is at certain value, and mixing feeds bioreactor main body 4 after filtering bacterium by strainer 21.Can directly feed the gas of accommodation in actual applications.
Described DO measures probe 25 and pH pH-value determination pH probe 24 and temp probe 22 and is installed in reactor head, is deep into below leakage fluid dram 405 positions.
Four, description of drawings
Fig. 1 is the continuous operational system synoptic diagram of the present invention;
Fig. 2 is a bioreactor main body front view of the present invention;
Fig. 3 is a whipping appts synoptic diagram of the present invention;
Fig. 4 is a reactor top board synoptic diagram of the present invention.
Reference numeral: 1-gas distributor; The 2-light source controller; The 3-light source; 4-bioreactor main body; The 5-whipping appts; 6-reactor top board; The 7-air compressor; 8-CO 2Gas cylinder; The 9-gas meter; The 10-gas mixer; The 11-pH meter; The 12-dissolved oxygen meter; 13-stirs pump controller; The 14-temperature controller; The 15-gas treating system; 16-algae water separator; The 17-recycle pump; The 18-time controller; The 19-fluid filling pump; The 20-container for storing liquid; The 21-strainer; The 22-temp probe; The 23-blower fan; The 24-pH probe; The 25-DO probe;
The 401-fluid inlet; The 402-area of illumination; The 403-thief hole; 404-gas-liquid separation and DATA REASONING district; The 405-leakage fluid dram;
501-U type stirring-head; The 502-contiguous block; The 503-sealing bearing; The 504-reducing; 505-stirs pump; 506-stirs the pump support;
The 601-venting port; 602-stirs pump support reinforcement block; The 603-screw hole; The 604-air inlet port; The 605-pH open holes of popping one's head in; The 606-DO open holes of popping one's head in; 607-temp probe open holes.
Five, embodiment
The present invention mainly comprises bioreactor main body, illumination system, airing system, nutritive medium loading system, stirring system, temperature measuring and Controlling System, DO, pH determinator composition.Wherein bioreactor main body 4 adopt transmittances preferably material make, be divided into area of illumination 402 and gas-liquid separation and DATA REASONING district 404, gas-liquid separation and 404 middle parts, DATA REASONING district are provided with leakage fluid dram 405, and the algae water mixed liquid enters algae water separator 16 from here.The area of illumination mid-way is provided with thief hole 403, and the bottom is provided with 401 mouthfuls of feed liquors; Stirring system is made up of gas distributor 1, stirring pump controller 13, stirring pump 505, reducing 504, sealing bearing 503, contiguous block 504, U type stirring-head 501 (3); Illumination system is made up of light source 3 and light source controller 2; The nutritive medium loading system forms 20 by time controller 18, surge pump (recycle pump 17 and fluid filling pump 19), nutritive medium storage tank; Temperature controlling system is made up of temp probe 22, temperature controller 14 and blower fan 23; Airing system is by CO 2Gas cylinder 8, air compressor pump 7, gas meter 9, gas mixer 10 and strainer 21 are formed; Reactor head is provided with the mensuration probe 24 that DO measures probe 25 and pH value.
When moving continuously, the nutritive medium of preparation pulsed filling under the control of time controller 18 in advance by fluid filling pump 19 and recycle pump 17; Pass through CO 2Gas cylinder 8 and air compressor pump 7 utilize the Atmospheric composition under meter 9 virtual space cabins, control CO 2Concentration is at certain value, mixes to feed bioreactor after filtering bacterium by strainer 21, and the mechanical stirring by U type stirring-head 501 makes it and algae water mixed liquid uniform mixing, promotes mass transfer; Required illuminance is by light source 3 and light source controller 2 controls; The algae water mixed liquid is discharged by leakage fluid dram 505 and is entered algae water separator 16, and gas is discharged by venting port 601 and entered gas treatment equipment 15.The present invention is used for the bioreactor of spacing regenerative oxygen, has realized the higher efficiency of light energy utilization by the led light source of design voluntarily, and the whipping appts of employing can make CO 2Contact fully with microalgae cell, help photosynthetic carrying out, adopt the pulsed liquid feeding to realize continuous high-density culture and the O of little algae 2Regeneration.

Claims (4)

1, a kind of bioreactor that is used for spacing regenerative oxygen comprises bioreactor main body, illumination system, airing system, nutritive medium loading system, stirring system, temperature measuring and Controlling System.
2, the bioreactor that is used for spacing regenerative oxygen according to claim 1 is characterized in that the bioreactor main body is divided into area of illumination and gas-liquid separation and DATA REASONING district.Area of illumination aspect ratio 2.5-5, illuminating area and volume of culture ratio (S/V) are at 20-50m -1
3, the bioreactor that is used for spacing regenerative oxygen according to claim 1, it is characterized in that stirring system adopts dual stirring: (1) mechanical stirring, adopt softer U type stirring-head, stirring velocity can reach 200r/min, can be used for the thin Spirulina of culturing cell wall etc., can not cause the fragmentation of alga cells, realize the thorough mixing of solid-liquid gas simultaneously, and guarantee effective utilization of luminous energy.(2) gas stirring, air flow 20L/hL.
4, the bioreactor that is used for spacing regenerative oxygen according to claim 1, it is characterized in that the nutritive medium loading system carries out the pulsed filling of nutritive medium by the working order of time controller Control Circulation pump and fluid filling pump, liquid volume added can be controlled at 0.001-0.01L/L as requested, the timed interval can be controlled at 1-12 hour, had realized the cultured continuously of little algae.
CNA2006101620914A 2006-12-12 2006-12-12 Optical bioreactor for spacing regenerative oxygen Pending CN101033449A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNA2006101620914A CN101033449A (en) 2006-12-12 2006-12-12 Optical bioreactor for spacing regenerative oxygen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNA2006101620914A CN101033449A (en) 2006-12-12 2006-12-12 Optical bioreactor for spacing regenerative oxygen

Publications (1)

Publication Number Publication Date
CN101033449A true CN101033449A (en) 2007-09-12

Family

ID=38730191

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA2006101620914A Pending CN101033449A (en) 2006-12-12 2006-12-12 Optical bioreactor for spacing regenerative oxygen

Country Status (1)

Country Link
CN (1) CN101033449A (en)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101724546A (en) * 2009-10-29 2010-06-09 复旦大学 Alga and zooplankton linkage culturing device
CN101781663B (en) * 2009-01-21 2012-05-02 新奥科技发展有限公司 Method for preparing and collecting oxygen-enriched air
CN102443536A (en) * 2010-10-09 2012-05-09 新奥科技发展有限公司 Auxiliary device for photobioreactor
TWI381046B (en) * 2009-09-28 2013-01-01 Univ Ishou Airlift type light reaction device
CN103109752A (en) * 2013-01-05 2013-05-22 北京航空航天大学 Experimental device of minitype creature regenerative type life support system
CN103698158A (en) * 2013-12-27 2014-04-02 安徽淮化股份有限公司 Solution sampling stirrer
CN103885468A (en) * 2013-09-27 2014-06-25 北京航空航天大学 Controller for regulating and controlling artificial closed ecological system gas balance by utilizing microalgae and aerobic fermentation
CN104214845A (en) * 2014-09-15 2014-12-17 宋昊 Microalgae oxygen bar-mediated air purification device
CN106754342A (en) * 2017-01-17 2017-05-31 杭州元点生物科技有限公司 A kind of equipment of the culture spirulina of high-purity indoors of closed
CN107177476A (en) * 2016-03-10 2017-09-19 空中客车Ds有限责任公司 Transfer device
CN109516651A (en) * 2018-12-29 2019-03-26 温州市龙湾建设园林绿化工程有限公司 A kind of gardens water purification system and its purification method
CN109642193A (en) * 2016-08-26 2019-04-16 株式会社Ihi Cell culture apparatus and cell culture processes
CN114317269A (en) * 2022-03-09 2022-04-12 苏州大学 Multi-organ chip and application thereof in drug evaluation

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101781663B (en) * 2009-01-21 2012-05-02 新奥科技发展有限公司 Method for preparing and collecting oxygen-enriched air
TWI381046B (en) * 2009-09-28 2013-01-01 Univ Ishou Airlift type light reaction device
CN101724546A (en) * 2009-10-29 2010-06-09 复旦大学 Alga and zooplankton linkage culturing device
CN102443536A (en) * 2010-10-09 2012-05-09 新奥科技发展有限公司 Auxiliary device for photobioreactor
CN102443536B (en) * 2010-10-09 2014-07-30 新奥科技发展有限公司 Auxiliary device for photobioreactor
CN103109752A (en) * 2013-01-05 2013-05-22 北京航空航天大学 Experimental device of minitype creature regenerative type life support system
CN103885468B (en) * 2013-09-27 2016-08-17 北京航空航天大学 A kind of method for designing based on the controller regulating gas balance in artificial closed ecological system
CN103885468A (en) * 2013-09-27 2014-06-25 北京航空航天大学 Controller for regulating and controlling artificial closed ecological system gas balance by utilizing microalgae and aerobic fermentation
CN103698158A (en) * 2013-12-27 2014-04-02 安徽淮化股份有限公司 Solution sampling stirrer
CN103698158B (en) * 2013-12-27 2015-10-28 安徽淮化股份有限公司 A kind of solution sampling stirrer
CN104214845A (en) * 2014-09-15 2014-12-17 宋昊 Microalgae oxygen bar-mediated air purification device
CN107177476A (en) * 2016-03-10 2017-09-19 空中客车Ds有限责任公司 Transfer device
CN109642193A (en) * 2016-08-26 2019-04-16 株式会社Ihi Cell culture apparatus and cell culture processes
CN106754342A (en) * 2017-01-17 2017-05-31 杭州元点生物科技有限公司 A kind of equipment of the culture spirulina of high-purity indoors of closed
CN109516651A (en) * 2018-12-29 2019-03-26 温州市龙湾建设园林绿化工程有限公司 A kind of gardens water purification system and its purification method
CN114317269A (en) * 2022-03-09 2022-04-12 苏州大学 Multi-organ chip and application thereof in drug evaluation
CN114317269B (en) * 2022-03-09 2022-05-31 苏州大学 Multi-organ chip and application thereof in drug evaluation

Similar Documents

Publication Publication Date Title
CN101033449A (en) Optical bioreactor for spacing regenerative oxygen
Ugwu et al. Photobioreactors for mass cultivation of algae
AU2006324198B2 (en) A carbon supply device for cultivating miro algae in large and its application method and use
EP2672807B1 (en) Method and bioreactor for the cultivation of microorganisms
EP3328985A1 (en) Light emitting diode photobioreactors and methods of use
KR101043583B1 (en) Photobioreactor having baffles integrally provided with internal light source for high density cultivation of microalgae
Muller-Feuga et al. Comparison of artificial light photobioreactors and other production systems using Porphyridium cruentum
CN110760439B (en) Algae cultivation photo-biological reaction kettle and continuous culture reaction system containing same
CN101985595A (en) Membrane type photobioreactor for enhancing microalgae culturing
CN104988059A (en) Photobioreactor for algal culture
CN105002085A (en) Culture farm special-purpose microalgae photo-biological culture system and culture method
CN204474658U (en) Multistage potential energy differential driving microdisk electrode dedicated optical bioreactor system
CN201040232Y (en) Photobioreactor used for spacing regeneration oxygen
CN2688718Y (en) Spiral pipe optical biological reactor
Tsygankov Laboratory scale photobioreactors
CN204737957U (en) A photobioreactor for algae culture
CN2394915Y (en) Bubble tower type light biological reactor
WO2016000192A1 (en) Bioreactor with built-in light source and microalgae culture method
WO2014130357A1 (en) Bioreactor array and methods of combinatorial testing
CN104593224B (en) Special photo-biological reactor system for driving microalgae cultivation by multi-stage potential energy differences
EP2725090A1 (en) System for obtaining biomass
CN1472305A (en) Integral pneumatic lifting photobiological reactor and use thereof
JPH10150974A (en) Apparatus for culturing photosynthetic microorganism and culturing method
Griffiths 5 Microalgal Cultivation
KR101415553B1 (en) Device for culturing micro algae

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication