CN201040232Y

CN201040232Y - Photobioreactor used for spacing regeneration oxygen

Info

Publication number: CN201040232Y
Application number: CNU2006201660692U
Authority: CN
Inventors: 刘红; 杨晨亮
Original assignee: Beihang University
Current assignee: Beihang University
Priority date: 2006-12-12
Filing date: 2006-12-12
Publication date: 2008-03-26
Anticipated expiration: 2016-12-12

Abstract

The utility model discloses an optical bioreactor used for oxygen of spatial regeneration. The optical bioreactor comprises a body of optical bioreactor, a stirring device, an addition controlling device of nutrient solution, a CO2 supplying device, a lighting device, a temperature controlling device, a DO determination probe and a pH value determination probe. The body of the optical bioreactor consists of a lighting region, a separating region of gas and liquid and a data measuring region. The bottom of the optical bioreactor is provided with a gas distributing device. A U-shaped stirring head is arranged above the gas distributing device to realize the complete mixture of solid, liquid and gas and complete use of solar energy. The injection of the nutrient liquid is added through a time counter and a diaphragm pump. The utility model can be used in the research field of spatial biology, and has the advantages of simple structure, low spatial occupation rate, high biologic utilizing rate, fast photosynthesis and fast speed of producing oxygen.

Description

The bioreactor that is used for spacing regenerative oxygen

One, technical field

The utility model relates to a kind of bioreactor of regeneration of oxygen, is mainly used in CO in space station, spacelab, the permanent base at space (as the moon, Mars base) ₂Purification and O ₂Regeneration.

Two, background technology

Life-support systems are manned space flight key techniques, CO ₂Purification and O ₂Regeneration be key in this key.Little algae can utilize luminous energy to carry out photosynthesis regeneration O ₂Purify CO ₂Bioreactor is to influence micro algae growth and regeneration O as micro algae culturing device ₂The key factor of speed.Design an algae culture bioreactor that is suitable for long-term space flight, have crucial meaning for the safety and reliability that reduce space freight, raising closed system.

The design of bioreactor will be satisfied: simple in structure, be convenient to the assembling and the operation of space test, and efficiency of light energy utilization height maximally utilises energy, reduces the energy resource consumption of following long-term flight, is fit to the continuous High Density Cultivation of little algae.

One of key of bioreactor design is the illumination surface to volume ratio that will provide high, its objective is that raising light transmittance and frustule are to the efficiency of light energy utilization; Another key of design is to select suitable EGR for use, reduces the injury of shearing force to frustule; CO in addition ₂Dissolving and when gas-liquid mass transfer, photosynthetic oxygen evolution dissolved oxygen accumulate selection with gas transfer efficiency such as discharge in time and light source quality and form, all will great influence be arranged to the little algae of bioreactor high-efficient culture.

In life-support systems, be used at present airtight bioreactor that little algae cultivates mainly contain gas-lifting type (MELISSA, ESA) and board-like (BIOS-3, Russia).Airlift photobioreactor has been avoided the injury of shearing force, but illuminating area and volume ratio are less, how to increase illuminating area and volume ratio, and does not influence other performances, is the subject matter that needs solution.Board-like bioreactor is to assemble the simplest class reactor, be convenient to realize the cultivation of the little algae of high density, but mass transfer and heat transfer property is relatively poor.

Bioreactor will be obtained the high density high-efficient culture, and the influence factor of most critical is that luminous energy transforms, the continuous supply of gas exchange, nutrient source and the biological limits factor etc.There are many drawbacks in present widely used light source as sunshine, metal halid lamp, cold incandescent lamp etc., in order to improve conversion of solar energy, selects high light intensity, narrow spectrum, energy-conservation, reliable light source to have great importance.

Three, technology contents

Technical problem to be solved in the utility model is, provide a kind of simple in structure, the efficiency of light energy utilization is high, the culture density height is easy to control, can be used for the O in cabins such as space base ₂Regenerating unit.

For achieving the above object, the technical scheme of taking is:

The utility model mainly comprises bioreactor main body, agitating device, nutrient solution filling apparatus, CO ₂Feedway, illumination apparatus, temperature control equipment, DO measure probe, pH pH-value determination pH probe.

Described bioreactor main body 4 employing light transmittances material are preferably made, and are divided into area of illumination 402 and gas-liquid separation zone 404, and the ratio of height to diameter of area of illumination is at 2.5-5, and illuminating area and volume of culture ratio (S/V) are at 20-50m ^-1Reactor head is provided with exhaust outlet 601, and from then on gas discharged and entered gas handling system 15.Gas-liquid separation zone 4 middle parts are provided with leakage fluid dram 405, and the algae water mixed liquid enters algae separator 16 from here.The centre position of area of illumination is provided with sample tap 403, and the bottom is provided with inlet 401.

Described agitating device is made up of gas distributor 1, stirring pump controller 13, stirring pump 505, reducing 504, sealed bearing 503, contiguous block 502, U type stirring-head 501 (3).Gas enters reactor by gas distributor 1, and the bubble rising realizes the stirring to the algae water mixed liquid, throughput 20L/ (hL) simultaneously; Gas distributor 1 top is a U type stirring-head 501, U type stirring-head 501 usefulness stainless steel wires are made core, make the U type behind the parcel plastic tube and be fixed in contiguous block 502, link to each other with stirring pump 505 main shafts again, mixing speed is regulated by stirring pump controller 13, and mixing speed can reach 200r/min, can be used for the thin Spirulina of cultured cell wall etc., can not cause the fragmentation of alga cells, realize CO simultaneously ₂With the abundant mixing of algae water mixed liquid, and guarantee fully contacting of frustule and light source, reach making full use of of light source.

Described illumination apparatus is made up of light source 3 and light source controller 2, adopts the high light emitting diode of present light efficiency as light source, and emission spectrum is narrower but can cover algae and carry out photosynthetic spectral region; Be arranged in around the reactor by dot matrix, utilize light source controller 2 control intensities of illumination.

Described nutrient solution filling apparatus is made up of time controller 18, membrane pump (circulating pump 17 and fluid filling pump 19), nutrient solution storage tank 20.Circulation and the nutrient solution of duty realization algae water mixed liquid between sample tap 403 and inlet 401 by time controller 18 control circulating pumps 17 and fluid filling pump 19 annotated to the pulsed of reactor from storage tank, prevents algae adhering at closed circuit simultaneously.Liquid volume added can be controlled at 0.001-0.01L/L as requested, and the time interval can be controlled at 1-12 hour.

Described temperature control equipment is made up of temp probe 22, temperature controller 14 and blower fan 23.Blower fan 23 is started working cold air is blown to light source 3 and reactor surface when temperature of reactor surpasses certain value, take away more than heat, realize temperature controlling.

Described CO ₂Feedway is by CO ₂Gas cylinder 8, air pressure pump 7, gas flowmeter 9, gas mixer 10 and filter 21 are formed.Pass through CO ₂Gas cylinder 8 and air pressure pump 7 utilize the Atmospheric composition in flowmeter 9 virtual space cabins, control CO ₂Concentration is at certain value, and mixing feeds bioreactor main body 4 after filtering bacterium by filter 21.Can directly feed the gas of accommodation in actual applications.

Described DO measures probe 25, pH pH-value determination pH probe 24 and temp probe 22 and is installed in reactor head, is deep into below leakage fluid dram 405 positions.

Four, description of drawings

Fig. 1 is the continuous operational system schematic diagram of the utility model;

Fig. 2 is the utility model bioreactor main body front view;

Fig. 3 is the utility model mechanical stirring device schematic diagram;

Fig. 4 is the utility model reactor top board schematic diagram.

Reference numeral: 1-gas distributor; The 2-light source controller; The 3-light source; 4-bioreactor main body; The 5-agitating device; 6-reactor top board; The 7-air compressor; 8-CO ₂Gas cylinder; The 9-gas flowmeter; The 10-gas mixer; The 11-pH meter; The 12-dissolved oxygen meter; 13-stirs pump controller; The 14-temperature controller; The 15-gas handling system; 16-algae separator; The 17-circulating pump; The 18-time controller; The 19-fluid filling pump; The 20-fluid reservoir; The 21-filter; The 22-temp probe; The 23-blower fan; 24-pH pH-value determination pH probe; 25-DO measures probe;

The 401-inlet; The 402-area of illumination; The 403-sample tap; The 404-gas-liquid separation zone; The 405-leakage fluid dram;

501-U type stirring-head; The 502-contiguous block; The 503-sealed bearing; The 504-reducing; 505-stirs pump; 506-stirs the pump support;

The 601-exhaust outlet; 602-stirs pump support reinforcement block; The 603-screw hole; The 604-air admission hole; 605-pH pH-value determination pH probe installing hole; 606-DO measures the probe installing hole; 607-temp probe installing hole.

Five, the specific embodiment

The utility model mainly comprises bioreactor main body, agitating device, nutrient solution filling apparatus, CO ₂Feedway, illumination apparatus, temperature control system, DO measure probe, pH pH-value determination pH probe.Wherein bioreactor main body 4 adopt light transmittances preferably material make, be divided into area of illumination 402 and gas-liquid separation zone 404, gas-liquid separation zone 404 middle parts are provided with leakage fluid dram 405, the algae water mixed liquid enters algae separator 16 from here.The centre position of area of illumination is provided with sample tap 403, and the bottom is provided with 401 mouthfuls of feed liquors; Agitating device is made up of gas distributor 1, stirring pump controller 13, stirring pump 505, reducing 504, sealed bearing 503, contiguous block 504, U type stirring-head 501 (3); Illumination apparatus is made up of light source 3 and light source controller 2; The nutrient solution filling apparatus forms 20 by time controller 18, membrane pump (circulating pump 17 and fluid filling pump 19), nutrient solution storage tank; CO ₂Feedway is by CO ₂Gas cylinder 8, air pressure pump 7, gas flowmeter 9, gas mixer 10 and filter 21 are formed; Temperature control equipment is made up of temp probe 22, temperature controller 14 and blower fan 23; Reactor head is provided with DO and measures probe 25 and pH pH-value determination pH probe 24.

When moving continuously, nutrient solution pulsed filling under the control of time controller 18 by fluid filling pump 19 and circulating pump 17; Pass through CO ₂Gas cylinder 8 and air pressure pump 7 utilize the Atmospheric composition in flowmeter 9 virtual space cabins, control CO ₂Concentration, filter 21 feeds bioreactor after filtering bacterium, and the mechanical agitation by U type stirring-head 501 makes it evenly to mix with the algae water mixed liquid, promotes mass transfer; Required intensity of illumination is by light source 3 and light source controller 2 controls; The algae water mixed liquid is discharged by leakage fluid dram 505 and is entered algae separator 16, and gas is discharged by exhaust outlet 601 and entered gas treatment equipment 15.The utility model is used for the bioreactor of spacing regenerative oxygen, can realize the higher efficiency of light energy utilization by led light source and agitating device, has guaranteed CO ₂Contact fully with microalgae cell, help photosynthetic carrying out, product oxygen speed is fast, and the employing of pulsed liquid feeding has realized continuous High Density Cultivation and the O of little algae ₂Regeneration.

Claims

1. a bioreactor that is used for spacing regenerative oxygen comprises bioreactor main body, agitating device, nutrient solution filling apparatus, CO ₂Feedway, illumination apparatus, temperature control equipment, DO measure probe, pH pH-value determination pH probe, it is characterized in that: bioreactor main body (4) adopts light transmissive material to make, and is provided with exhaust outlet (601), leakage fluid dram (405), inlet (401) and sample tap (403); Stir pump (505) and be fixed on bioreactor main body (4) top, stir pump (505) and drive U type stirring-head (501) realization stirring by support (506); Bioreactor main body (4) is light source (3) on every side, links to each other with light source controller (2) by lead; The duty of circulating pump (17) and fluid filling pump (19) is by time controller (18) control, and nutrient solution is annotated to reactor by fluid filling pump (19) from fluid reservoir (20); Blower fan (23) is by temp probe (22), temperature controller (14) control; CO ₂By CO ₂Gas cylinder (8) and air pressure pump (7) provide CO ₂Concentration feeds bioreactor main body (4) by flowmeter (9) control by filter (21) back; DO measures probe (25), pH pH-value determination pH probe (24) and temp probe (22) and is installed in the bioreactor top.

2. the bioreactor that is used for spacing regenerative oxygen according to claim 1 is characterized in that the bioreactor main body is divided into area of illumination and gas-liquid separation zone.

3. the bioreactor that is used for spacing regenerative oxygen according to claim 1 is characterized in that the bioreactor bottom part body is provided with gas distributor, and the gas distributor top is provided with U type stirring-head.

4. the bioreactor that is used for spacing regenerative oxygen according to claim 1 is characterized in that the nutrient solution filling apparatus is made up of time controller, membrane pump.