CN101026991A - 用于检测异常上皮组织的方法 - Google Patents

用于检测异常上皮组织的方法 Download PDF

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CN101026991A
CN101026991A CNA2004800440947A CN200480044094A CN101026991A CN 101026991 A CN101026991 A CN 101026991A CN A2004800440947 A CNA2004800440947 A CN A2004800440947A CN 200480044094 A CN200480044094 A CN 200480044094A CN 101026991 A CN101026991 A CN 101026991A
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light
epithelial tissue
preselected wavelength
tissue
wavelength
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马克·布赖德
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Zila Pharmaceuticals Inc
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/44—Detecting, measuring or recording for evaluating the integumentary system, e.g. skin, hair or nails
    • A61B5/441—Skin evaluation, e.g. for skin disorder diagnosis
    • A61B5/444—Evaluating skin marks, e.g. mole, nevi, tumour, scar
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/44—Detecting, measuring or recording for evaluating the integumentary system, e.g. skin, hair or nails
    • A61B5/441—Skin evaluation, e.g. for skin disorder diagnosis
    • A61B5/445—Evaluating skin irritation or skin trauma, e.g. rash, eczema, wound, bed sore
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
    • A61B5/0088—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for oral or dental tissue

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  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
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  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Endoscopes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

本发明提供了用于检测异常上皮组织的方法。具体地说，在具有选择性地鉴定异常组织的波长峰的光下，通过传播所述波长峰但阻断其它波长传播的透镜来观察所述组织，加强了普通环境光存在时异常组织的可视性。

Description

用于检测异常上皮组织的方法
技术领域
本发明涉及用于检测异常上皮组织的方法，所述上皮组织可能具有肿瘤表型。
另一方面，本发明涉及用于体内实时检查上皮组织以检测异常的改进方法，所述异常可以是癌性的或者可以最终发展为侵袭癌。
背景技术
耽搁至少两个月才获得癌症会诊的患者，其所面临的死亡相对危险显著高于耽搁较短时间的患者。因此，如果患者较经常地接受有效的癌症筛查，那么癌症死亡风险率将得以降低。因此，需要一种用于检测异常粘膜组织的简便快速的筛查试验，所述异常粘膜组织可能具有肿瘤表型，该表型可能指示侵袭癌的存在或者最终发展。
使用选择性光检查(其非常适合于作为常规医疗和牙科检查的辅助手段而进行快速低廉的筛查)可以对异常上皮组织进行体内实时目测鉴定和定位。作为示例，美国专利5,179,938和5,329,938(在此以参考的方式引入)描述了配有化学发光光源的仪器，所述化学发光光源在可见的绿色光谱、蓝色光谱和任选的红色光谱内发光，其谱峰为450nm、550nm和580nm。在这样的照明下，普通环境光受到抑制，异常粘膜组织呈现白色。作为示例，用于实施所述体内检查的所述选择性光装置可以从ZilaPharmaceuticals，Inc.，Phoenix，Arizona，USA商购获得，其注册商标为VIZILITE。
使用所述光源对异常粘膜组织进行选择性可视化会受到落在被检组织上的普通环境光(日光或普通人工光)的妨碍，使得用于进行所述检查的标准程序要求使在其中进行该检查的房间变暗。这不仅很麻烦，而且在窗户面积大的房间内进行所述检查时或者在供以公共常规照明的同一房间内有其它患者正在进行其它程序时，这将是不可能的。
发明内容
本发明的主要目的是提供用于进行所述选择性光检查的方法，无需使在其中进行所述检查的房间变暗就可以实施该方法。本发明人现已经发现了普通环境光存在时可以实施的选择性光检查法。
简而言之，本发明用于筛查上皮组织以检测可能的异常组织，其包括用预选波长的光照射上皮组织的总解剖学区域(gross anatomical area)，并通过滤光镜观察被照射的总组织区域，所述预选波长的光选择性地帮助使所述总区域上的异常组织部位可视化，所述滤光镜仅透射所述预选波长的光，同时基本上阻断具有除所述预选波长以外的波长的环境光的传播，因此加强了普通环境光存在时任何异常组织部位的选择性可视化。
具体实施方式
提供以下实施例以使本领域技术员能够理解和实施本发明，并确定本发明目前优选的实施方式。提供这些实施例是为说明目的，而不是用以表示本发明的范围，本发明的范围仅由所附权利要求限定。
实施例1
对口腔进行常规目测检查，注意附着龈、口腔粘膜、牙床、硬腭和软腭以及舌的背部、侧部和腹部上的任何病变的存在。记录通过所述常规检查所注意到的任何病变。
在完成实施例1的所述常规检查以后，让患者用1％的醋酸溶液冲洗口腔至多1分钟，然后吐出。
通过使柔性外囊弯曲，使脆性的内瓶破碎，从而激活Lonky的美国专利5,329,938所述的、可以以VIZILITE为注册商标而商购获得的化学发光光源。然后摇动所述外囊，并将其插入牵引器中。所提供的光在约450nm和550nm具有谱峰并在约600nm的红区具有较小的峰，如图1所示。这些谱峰形成白蓝色的光。
然后，临床检查人员戴上一副眼镜，该眼镜提供有仅透射400nm～600nm波段的光的透镜，如图2所示。所述眼镜具有使从上方和侧面到达检查人员眼睛的照明最小的形状。所述眼镜可以从ZilaPharmaceuticals，Inc.，Phoenix，Arizona商购获得，其注册商标为VIZILITE。
然后，无需降低来自普通照明光源的环境光，利用所述光源提供的照明，再次对口腔进行目测检查，查找病变或其它呈现白色的可疑组织部位，特别留意在实施例1的所述常规检查中已注意到的任何可疑组织部位。注意并记录呈现白色或白蓝色的任何部位。
对所注意的部位进行进一步的评测，例如通过用于标准组织学的组织活检或者通过分子分析进行进一步的评测，以确定所述组织是否为癌性或具有位于最终发展为侵袭癌的进程中的突变。
为了让本领域技术人员能够理解并实施本发明而以所述描述方式对本发明进行了说明，并确定了本发明目前优选的实施方式。

Claims (1)

1.一种筛查上皮组织以检测可能的异常组织部位的方法，该方法包括：
(a)用预选波长的光照射上皮组织的总解剖学区域，所述预选波长的光选择性地帮助使所述总区域上的异常组织部位可视化；和
(b)通过滤光镜观察所述总区域，所述滤光镜传播所述预选波长的光，同时基本上阻断具有除所述预选波长以外的波长的光的传播，从而加强普通环境光存在时任何所述异常组织部位的可视化。
CNA2004800440947A 2004-09-28 2004-09-28 用于检测异常上皮组织的方法 Pending CN101026991A (zh)

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PCT/US2004/031963 WO2006036149A1 (en) 2004-09-28 2004-09-28 Methods for detecting abnormal epithelial tissue

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EP (1) EP1793727A4 (zh)
JP (1) JP2008514272A (zh)
CN (1) CN101026991A (zh)
AU (1) AU2004323582A1 (zh)
BR (1) BRPI0419095A (zh)
CA (1) CA2549726A1 (zh)
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US20100256125A1 (en) * 2009-04-06 2010-10-07 Zila Pharmaceuticals, Inc. Use of improved toluidine blue in photodynamic therapy
EP3664686A4 (en) 2017-08-07 2021-04-21 Weinmann, Maxwell LARYNGOSCOPE

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CA2549726A1 (en) 2006-04-06
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MX2007003619A (es) 2007-08-02
AU2004323582A1 (en) 2006-04-06
US20060241494A1 (en) 2006-10-26
EP1793727A4 (en) 2009-01-07
JP2008514272A (ja) 2008-05-08
EP1793727A1 (en) 2007-06-13

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