CN101019510A - Production process of erectum - Google Patents
Production process of erectum Download PDFInfo
- Publication number
- CN101019510A CN101019510A CNA2007100676480A CN200710067648A CN101019510A CN 101019510 A CN101019510 A CN 101019510A CN A2007100676480 A CNA2007100676480 A CN A2007100676480A CN 200710067648 A CN200710067648 A CN 200710067648A CN 101019510 A CN101019510 A CN 101019510A
- Authority
- CN
- China
- Prior art keywords
- erectum
- bud
- producing
- medium
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to erectum producing process, and is especially process of producing erectum with high hypericin content. The industrial erectum producing process with low cost and high quality includes the following steps: washing seed of erectum, sterilizing with mercury bichloride, culturing in MS culture medium containing cane sugar and agar as well as NAA and 6-BA in certain concentration to obtain cespitose bud, culturing the cespitose bud in the proliferating culture medium to proliferate, culturing the cespitose bud in the rooting culture medium to root and grow so as to obtain erectum seedling with high hypericin content. The culturing process is preferably a lighting process at 20-30 deg.c.
Description
Technical field
The present invention relates to a kind of method of producing hypericum erectum, particularly a kind of method of producing the high hypericum erectum of hypericin content.
Technical background
Hypericum erectum is the Guttiferae perennial herb, and all herbal medicine can be treated internal haemorrhage, hepatitis, swollen sore, arthralgia due to wind-dampness, premenstrual abdominal pain, stomachache, traumatic injury, venomous snake bite, the tool antirheumatic, antibiotic, diuresis, anti-inflammatory analgetic, convergence, hemostasia and promoting granulation, menstruation regulating, effects such as lactagogue mainly are distributed in China East China and Taiwan Province.Contained hypericin and pseudohypericin in the hypericum erectum wherein is the active substance of tool representative in this plant.Recently discover that hypericin has significant anti-DNA, RNA viruses effect, can be used for the treatment of acquired immune deficiency syndrome (AIDS),, caused the rapid minimizing of hypericum wild resource because hypericin has multiple pharmacological effect and its increase in American-European demand.Its wild plant has been used for commodity production; but the quality of these products may be subjected to varying environment; pollute; fungi; bacterium, the influence of insect etc., thus the accumulation that changes medicinal metabolite is effective protection wild resource; avoid resource exhaustion, hypericum is carried out cultured in vitro, plant regeneration just seem very necessary.
The existing raw material of producing hypericin mainly is a hypericum perforatum, and other species resource of this genus is lacked research and development.It is reported that the hypericin content of the open-air seedling of hypericum erectum is the highest in hypericum, hypericin content is 0.016% in its middle period, and the content in the hypericum perforatum leaf is 0.005%, so hypericum erectum be carry out tissue culture suitability for industrialized production hypericin than good material, and the report of producing the hypericum erectum seedling-growing method is not arranged as yet at present.
Summary of the invention
The purpose of this invention is to provide a kind of produce hypericum erectum method, a kind of cost is low, the measured raw material of matter for the suitability for industrialized production hypericin provides.
The method of production hypericum erectum provided by the present invention, be seed with the hypericum hypericum erectum with flushing with clean water after, behind mercuric chloride sterilization 2～5min, receive in the MS medium of 6-BA of the NAA that is mixed with 0.1～1.0mg/l concentration that contains 2.5～3.5% sucrose, 0.5～1.5% agar and 1.0～5.0mg/l concentration and carried out tissue culture 20～45 days; To in proliferated culture medium, carry out enrichment culture 10～30 days to the bud grafting of growing thickly of inducing gained; Then the bud grafting of growing thickly through enrichment culture is carried out culture of rootage 20～45 days in root media, treat that it takes root, grow tall, be the higher seedling of taking root of hypericin content.Wherein, proliferated culture medium is the MS medium that is mixed with the 6-BA of the NAA of 0.2～2.0mg/l concentration and 1.0～5.0mg/l concentration; Root media is the 1/2MS medium.Described percentage is mass percent; In order to obtain culture effect preferably, culture environment is that light is cultivated in 20～30 ℃ of temperature.
Embodiment
Below by embodiment the present invention is made further and to specify, but the invention is not restricted to these embodiment.
Example 1
A kind of step of producing hypericum erectum:
1, with the seed flushing with clean water of hypericum erectum, behind mercuric chloride sterilization 2min, receive in the MS medium of 6-BA of the NAA that is mixed with 0.1mg/l concentration that contains 2.5% sucrose, 0.5% agar and 1.0mg/l concentration and carry out tissue culture, light was cultivated 20 days in 20 ℃ of environment, induced to produce the bud of growing thickly;
2, the above-mentioned bud of growing thickly is transferred to carries out enrichment culture in the proliferated culture medium, wherein proliferated culture medium is the MS medium that is mixed with the 6-BA of the NAA of 0.2mg/l concentration and 5.0mg/l concentration, carries out enrichment culture 10 days then in 30 ℃ of environment.
3, the above-mentioned bud of growing thickly is transferred to carries out culture of rootage in the 1/2MS medium, light was cultivated 20 days in 30 ℃ of environment, treated that it takes root, and grew tall, and promptly became the higher seedling of taking root of hypericin content.
Example 2
A kind of step of producing hypericum erectum:
1, with the seed flushing with clean water of hypericum erectum, behind mercuric chloride sterilization 5min, receive in the MS medium of 6-BA of the NAA that is mixed with 1.0mg/l that contains 3.5% sucrose, 1.5% agar and 5.0mg/l and carry out tissue culture, light was cultivated 45 days in 26 ℃ of environment, induced to produce the bud of growing thickly.
2, the above-mentioned bud of growing thickly is transferred to carries out enrichment culture in the proliferated culture medium, wherein proliferated culture medium is the MS medium that is mixed with the 6-BA of the NAA of 2.0mg/l and 1.0mg/l, carries out enrichment culture 30 days then in 26 ℃ of environment.
3, the above-mentioned bud of growing thickly is transferred to carries out culture of rootage in the 1/2MS medium, light was cultivated 45 days in 26 ℃, treated that it takes root, and grew tall, and was the higher seedling of taking root of hypericin content.
Claims (3)
1, a kind of method of producing hypericum erectum is characterized in that the method that the present invention produces hypericum erectum may further comprise the steps:
A, with the seed flushing with clean water of hypericum erectum, behind mercuric chloride sterilization 2～5min, receive in the MS medium of 6-BA of the NAA that is mixed with 0.1～1.0mg/l concentration that contains 2.5～3.5% sucrose, 0.5～1.5% agar and 1.0～5.0mg/l concentration and carry out tissue culture, light was cultivated 20～45 days in 20～30 ℃ of environment, induce and produce the bud of growing thickly, wherein, described percentage is mass percent;
B, the above-mentioned bud of growing thickly is transferred in the proliferated culture medium, light was cultivated 10～30 days in 20～30 ℃ of environment of temperature;
C, the above-mentioned bud of growing thickly that carries out behind the enrichment culture is transferred in the root media, light was cultivated 20～45 days in 20～30 ℃ of environment of temperature, treated that it takes root, and grew tall, and promptly became the higher seedling of taking root of hypericin content.
2, a kind of method of producing hypericum erectum according to claim 1 is characterized in that proliferated culture medium is the MS medium that is mixed with the 6-BA of the NAA of 0.2～2.0mg/l concentration and 1.0～5.0mg/l concentration.
3, a kind of method of producing hypericum erectum according to claim 1 is characterized in that root media is the 1/2MS medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100676480A CN101019510A (en) | 2007-03-25 | 2007-03-25 | Production process of erectum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100676480A CN101019510A (en) | 2007-03-25 | 2007-03-25 | Production process of erectum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101019510A true CN101019510A (en) | 2007-08-22 |
Family
ID=38707442
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100676480A Pending CN101019510A (en) | 2007-03-25 | 2007-03-25 | Production process of erectum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101019510A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550270A (en) * | 2012-02-22 | 2012-07-11 | 陕西中医学院 | Method for ecological composite planting of Forsythia suspensa |
| CN102763600A (en) * | 2012-08-03 | 2012-11-07 | 郧县珺龙药业有限责任公司 | Forsythia suspensa planting method |
| CN104067861A (en) * | 2014-06-25 | 2014-10-01 | 桐城市景艺苗木有限公司 | Method for grafting Hypericum monogynum tree branch onto birchleaf pear tree |
| CN105684913A (en) * | 2016-04-27 | 2016-06-22 | 覃伟 | Tissue culture fast propagation method for garcinia paucinervis |
| CN109496841A (en) * | 2018-11-13 | 2019-03-22 | 中国林业科学研究院林业研究所 | A kind of Herba Hyperici Patuli quick breeding method for tissue culture |
| CN109874673A (en) * | 2019-04-03 | 2019-06-14 | 郑州大学 | Three leaf Fructus Forsythiae in vitro culture rapid propagation methods |
-
2007
- 2007-03-25 CN CNA2007100676480A patent/CN101019510A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550270A (en) * | 2012-02-22 | 2012-07-11 | 陕西中医学院 | Method for ecological composite planting of Forsythia suspensa |
| CN102550270B (en) * | 2012-02-22 | 2016-06-15 | 陕西中医学院 | A kind of method of ecological composite planting of Forsythia suspensa |
| CN102763600A (en) * | 2012-08-03 | 2012-11-07 | 郧县珺龙药业有限责任公司 | Forsythia suspensa planting method |
| CN104067861A (en) * | 2014-06-25 | 2014-10-01 | 桐城市景艺苗木有限公司 | Method for grafting Hypericum monogynum tree branch onto birchleaf pear tree |
| CN104067861B (en) * | 2014-06-25 | 2016-03-02 | 桐城市景艺苗木有限公司 | A kind of method of Hypericum Chinense grafting birchleaf pear tree |
| CN105684913A (en) * | 2016-04-27 | 2016-06-22 | 覃伟 | Tissue culture fast propagation method for garcinia paucinervis |
| CN109496841A (en) * | 2018-11-13 | 2019-03-22 | 中国林业科学研究院林业研究所 | A kind of Herba Hyperici Patuli quick breeding method for tissue culture |
| CN109496841B (en) * | 2018-11-13 | 2022-03-22 | 中国林业科学研究院林业研究所 | Tissue culture and rapid propagation method for golden silk plum |
| CN109874673A (en) * | 2019-04-03 | 2019-06-14 | 郑州大学 | Three leaf Fructus Forsythiae in vitro culture rapid propagation methods |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104686345A (en) | Tissue culture rapid propagation method of liquorice | |
| CN101019510A (en) | Production process of erectum | |
| CN109006472B (en) | Method for tissue culture and rapid propagation of ornamental plants by using carbon nano tubes with specific concentrations | |
| CN104106468B (en) | The quick breeding method for tissue culture of a kind of radix fici simplicissimae | |
| CN102119655A (en) | Natural light rapid breeding method for dendrobium officinale | |
| CN109220791A (en) | A kind of tissue culture method using bulb breeding Hipeastrum vittalum (L Her.) Herb.- Amaryllisvittata Ait | |
| CN105010147A (en) | Special culture medium for improving tissue culture propagation speed of haworthia succulent plants and tissue culture method | |
| CN102217551B (en) | Tissue culture quick reproduction method for dendrobium chrysotoxum lindl bud tips | |
| CN104686339A (en) | Method for constructing regeneration system of Magnolia officinalissubsp. biloba(Rehd. et Wils.) Law. | |
| CN106613079B (en) | production method of pinellia ternata seed stems | |
| CN105494098A (en) | Method for rapidly propagating Psammosilene tunicoides seedlings | |
| CN101926284B (en) | Monkshood-tuber tissue culture and rapid propagation method | |
| CN102860257B (en) | Houttuynia cordata aerial stem tissue culture rapid propagation method | |
| CN104686337A (en) | Tissue culture rapid propagation method of lilium | |
| CN104686329A (en) | Tissue culture method for Eucommia ulmoides Oliv. | |
| CN103798137A (en) | Tissue culture rapid propagation method of salvia miltiorrhiza bge | |
| CN107549018A (en) | Chinese mugwort tissue culture method | |
| CN109526746B (en) | Tissue culture method for petioles of hairyvein agrimony | |
| CN108142281A (en) | A kind of Cortex Eucommiae method for tissue culture | |
| CN103477976A (en) | Stem tissue culture seedling method of dendrobium candidum | |
| CN116868891A (en) | Method for detoxification culture of passion fruit stem tip of purple fruit | |
| CN102783420A (en) | Method for quickly breeding cordate houttuynia | |
| CN105123512A (en) | Breeding method of anoectochilus roxburghii | |
| CN104782494A (en) | Thymus mongolicus Ronn. tissue culture rapid propagation method | |
| CN111280058B (en) | Rapid breeding method of detoxified seedlings of stem tips of salvia miltiorrhiza bunge |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20070822 |