CN116868891A - Method for detoxification culture of passion fruit stem tip of purple fruit - Google Patents
Method for detoxification culture of passion fruit stem tip of purple fruit Download PDFInfo
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- CN116868891A CN116868891A CN202310263338.5A CN202310263338A CN116868891A CN 116868891 A CN116868891 A CN 116868891A CN 202310263338 A CN202310263338 A CN 202310263338A CN 116868891 A CN116868891 A CN 116868891A
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- 244000288157 Passiflora edulis Species 0.000 title claims abstract description 39
- 235000000370 Passiflora edulis Nutrition 0.000 title claims abstract description 36
- 238000001784 detoxification Methods 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 14
- 235000013399 edible fruits Nutrition 0.000 title claims description 14
- 238000012258 culturing Methods 0.000 claims abstract description 7
- 239000001963 growth medium Substances 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 230000035755 proliferation Effects 0.000 claims description 13
- 238000005520 cutting process Methods 0.000 claims description 11
- 230000006698 induction Effects 0.000 claims description 11
- 229920001817 Agar Polymers 0.000 claims description 9
- 229930006000 Sucrose Natural products 0.000 claims description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 9
- 239000008272 agar Substances 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 9
- 239000005720 sucrose Substances 0.000 claims description 9
- 238000005406 washing Methods 0.000 claims description 9
- 230000001105 regulatory effect Effects 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 239000008223 sterile water Substances 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 4
- 230000012010 growth Effects 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 235000019362 perlite Nutrition 0.000 claims description 3
- 239000010451 perlite Substances 0.000 claims description 3
- 239000012883 rooting culture medium Substances 0.000 claims description 3
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- 241000700605 Viruses Species 0.000 abstract description 22
- 230000000694 effects Effects 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000012364 cultivation method Methods 0.000 abstract description 3
- 241000685978 East Asian Passiflora virus Species 0.000 abstract 1
- 241000210029 Passiflora latent virus Species 0.000 abstract 1
- 241001096603 Telfairia mosaic virus Species 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 6
- 239000002609 medium Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 235000000088 Maracuja Nutrition 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000218996 Passiflora Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 2
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical group C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 1
- HDFLMPNKTHXEMR-UHFFFAOYSA-N 2,3,6,7-tetrahydro-s-indacene-1,5-dione Chemical compound C1=C2C(=O)CCC2=CC2=C1CCC2=O HDFLMPNKTHXEMR-UHFFFAOYSA-N 0.000 description 1
- CGIDKJRJBMFXKV-UHFFFAOYSA-N 6-n'-benzylpurine-6,6-diamine Chemical compound N1=CN=C2N=CN=C2C1(N)NCC1=CC=CC=C1 CGIDKJRJBMFXKV-UHFFFAOYSA-N 0.000 description 1
- 241000219925 Oenothera Species 0.000 description 1
- 235000004496 Oenothera biennis Nutrition 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
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- 238000004458 analytical method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 239000012882 rooting medium Substances 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a detoxification cultivation method of passion fruit stem tips. The invention breaks through the original limitation that only one virus can be removed, successfully removes the main viruses existing in the main production area of the passion fruit in Guizhou, and realizes the technical breakthrough. The method is simple to operate, can be used for culturing the detoxified female parent, has the detoxified effect of TeMV, PLV, EAPV and the detoxified rate of 100 percent, is applied and implemented in a secret form by 2 enterprises in Guizhou, and uses the detoxified seedling obtained by the detoxified technology as the female parent to carry out passion fruit seedling, thereby bringing economic benefits of more than 500 ten thousand yuan to the enterprises and being expected to have potential economic values of more than 1000 ten thousand in Guizhou province in future.
Description
Technical Field
The invention relates to the technical field of agriculture, in particular to a detoxification cultivation method of passion fruit stem tips of purple fruits.
Background
The passion fruit yield is higher, the yield measurement data can break through 2000 kg/mu, the national passion fruit mu yield in 2018 is 1146.2 kg, the average mu yield of passion fruit in Guizhou province is less than 500 kg, and the average mu yield in Guizhou province is less than 1/2 of the average domestic level. There are other reasons such as management level, but the poor quality of the seedlings is the most fundamental reason. The seedlings purchased in the Guizhou over 90 percent are not seedlings produced from regular production channels, the quality is poor, various viruses are carried, and the detection rates of TeMV (evening primrose mosaic virus), EAPV (eastern Asian Passiflora Caerulear virus) and PLV (passion Caerulear V) are respectively 100%, 81.9% and 74.3% through high-throughput sequencing of 105 parts of passion fruit leaves in the main production area of the Guizhou Passiflora Caerulear, so that the passion fruit in the main production area of the Guizhou is infected by viruses in different degrees and the viruses in partial areas are serious.
After passion fruit is infected with virus, leaves are in the form of flower leaf spots, deformity, yellowing, deformity, lignification and other symptoms of fruits, passion fruit virus diseases can seriously influence the growth of passion fruit, seedlings with virus can cause slow growth of plants, few fruits can appear when the plants with virus, the deformity rate of fruits is high, the water content of fruits is reduced and the like, and serious attenuation phenomena can appear.
Therefore, the Guizhou needs to continue to develop the passion fruit industry, the bottleneck and the root problem of the high-quality seedlings are solved, and the popularization of the high-quality healthy seedlings is imperative.
Disclosure of Invention
The invention aims to provide a detoxification cultivation method of passion fruit stem tips of purple fruits. The method can effectively remove TeMV, PLV and EAPV existing in passion fruit seedlings, and improve the yield and quality.
The technical scheme of the invention is as follows: a method for detoxification cultivation of passion fruit stem tips of purple fruits comprises the following steps:
(1) Axillary bud disinfection treatment: immersing the passion fruit axillary buds of the passion fruits in 75% ethanol for 30s, washing the passion fruit axillary buds with sterile water for 2 times, washing the passion fruit axillary buds for 2min each time, sterilizing the passion fruit axillary buds with 0.1% HgCl2 solution for 15min, and then washing the passion fruit axillary buds with sterile water;
(2) Axillary bud culture: the axillary buds after disinfection are subjected to water absorption and proliferation culture by using MS+2.0mg/L6-BA and 0.1mg/L NAA culture medium to obtain strong axillary buds;
(3) Peeling off the stem tip: cutting tender tips of the newly grown axillary buds, sterilizing in the step (1), and peeling off the stem tips of 0.6 mm; the smaller the stripped stem tip is, the better the detoxification effect is, but the stem tip is not easy to survive, the size of the stem tip of a former person is 1mm, but the invention reduces the size of the stem tip to 0.6mm on the premise of ensuring the survival rate, and is more beneficial to removing viruses of tissue culture seedlings.
(4) Induction culture: inoculating the stripped stem tip into an induction culture medium for induction culture, wherein after 30 days of culture, a cluster of green cluster-shaped buds appear;
(5) Proliferation culture: cutting the stem tip into sections after the stem tip grows to 5-7 cm, inoculating 0.4-0.6cm each section with one internode to MS+1.5mg/L6-BA and culturing for relay generation with 0.1 mg/LIBA;
(6) Rooting culture: selecting proliferation buds with the length of 2-3 cm, cutting off tender buds from a basal part, and inoculating the tender buds into a 1/2MS+IBA0.1 mg/L culture medium for rooting culture;
(7) Secondary stem tip detoxification culture: taking a robust tissue culture seedling, shearing off a stem section with a terminal bud about 3cm, and culturing according to the steps (3) - (6); the secondary detoxification can greatly reduce the survival rate under the condition of improving the detoxification effect, and the secondary detoxification can improve the detoxification rate to 100 percent, and the survival rate is maintained to be more than 70 percent. The survival rate is improved by about 30 percent when meeting the conventional secondary detoxification at present.
(8) Hardening and transplanting: when the root length of the tissue culture seedling is 2-3 cm, selecting the tissue culture seedling with the same growth vigor, the plant height of 5-7 cm and 4-5 main roots, placing the tissue culture bottle at the indoor non-sunlight direct irradiation position for hardening off for 5-7 days, taking out the residual culture medium of the root after hardening off, lightly cleaning the tissue culture seedling with flowing water, then transplanting the tissue culture seedling into a seedling pot, pouring enough rooting water, and growing new roots and new leaves after transplanting for 10-15 days to obtain the detoxified seedling.
The culture conditions are as follows: the culture temperature is 25+/-2 ℃, the illumination intensity is 2 000lx, the illumination time is 12h/d, and the relative humidity is 85-90%.
The composition of the induction culture medium takes MS as a basic culture medium, 4.74g/L MS powder, 30g/L sucrose, 7g/L agar, 1.0mg/L6-BA and 0.1mg/LNAA are added, and the pH value is regulated to 6.0.
The proliferation culture medium takes MS as a basic culture medium, 4.74g/L MS powder, 30g/L sucrose, 7g/L agar, 1.5mg/L6-BA and 0.1mg/LIBA are added, and the pH value is regulated to 6.0.
The rooting culture medium takes 1/2MS as a basic culture medium, wherein 2.47g/L MS powder, 30g/L sucrose, 7g/L agar and 0.1mg/LIBA are added, and the pH value is regulated to 6.0.
The seedling raising matrix is prepared by mixing turfy soil and perlite according to a mass ratio of 4:1.
The beneficial effects of the invention are as follows: compared with the background of the prior art, the invention breaks through the original limitation that only one virus can be removed, successfully removes the main viruses existing in the main production area of the passion fruit in Guizhou, and realizes the technical breakthrough. The method is simple to operate, can be used for culturing the detoxified female parent, and has the detoxified effect of TeMV, PLV, EAPV and 100 percent of detoxication rate through detection. Compared with the conventional detoxification at present, the method can remove three viruses simultaneously, and the removal rate of the three viruses reaches 100%, which can be a very important breakthrough.
Drawings
FIG. 1 shows the results of virus detection after the first detoxification of the present invention;
in fig. 1: m: DNA standard molecular weight; 1-9: the first time of stem tip detoxification tissue culture seedling; 10-12: tissue culture seedlings are not subjected to detoxification culture of stem tips; 13: blank control
FIG. 2 shows the effect of virus detection after a second detoxification according to the present invention;
in fig. 2: a: teMV, B: EAPV, C: PLV, M: a DNA marker;1-5: the stem tip detoxified tissue culture seedling; 6: tissue culture seedlings are not subjected to detoxification culture of stem tips; 7: blank control.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to be limiting.
Test site: laboratory, guizhou fruit tree science research laboratory, zhenfeng county, qian southwest, lu Rongxiang
Test time: 2019
Test materials: purple fruit passion fruit material selected from Zhenning base of Guizhou fruit tree science research institute
The experimental method comprises the following steps: a method for detoxification culture of the stem tip of purple passion fruit of Qian Xiang No. 1,
(1) Axillary bud disinfection treatment: fully immersing the armpit buds of the purple passion fruits of the 'Qian Xiang No. 1' in 75% ethanol for 30s, washing with sterile water for 2 times, washing for 2min each time, then sterilizing with 0.1% HgCl2 solution, and continuously shaking the beaker during the sterilization to enable the disinfectant to fully contact the materials, washing with sterile water for 6 times after 15min, and washing for 2min each time;
(2) Axillary bud culture: the axillary buds after disinfection are subjected to water absorption and proliferation culture by using an MS+2.0mg/L6-BA+0.1mg/L NAA culture medium to obtain strong axillary buds;
(3) Peeling off the stem tip: cutting tender tips of newly grown axillary buds, sterilizing, and peeling off 0.6mm stem tips under an anatomic lens;
(4) Induction culture: inoculating the stripped stem tip into an MS+1.0mg/L6-BA+0.1mg/LNAA culture medium for induction culture, and after culturing for 30 days, forming a cluster of green cluster buds;
(5) Proliferation culture: cutting into sections for subculture after stem tip grows to 5-7 cm, wherein each section is about 0.5cm and is provided with one internode, and inoculating to MS+1.5mg/L6-BA+0.1mg/LIBA for culture subculture;
(6) Rooting culture: selecting proliferation buds with the length of 2-3 cm, cutting off tender buds from a basal part, and inoculating the tender buds into a 1/2MS+IBA0.1 mg/L culture medium for rooting culture;
(7) Secondary stem tip detoxification culture: cutting off stem segments with terminal buds about 3cm from strong test tube plantlets, and culturing according to the steps (3) - (6);
(8) Hardening and transplanting: when the root length of the tissue culture seedling is 2-3 cm, selecting the tissue culture seedling with the same growth vigor, the plant height of 5-7 cm and 4-5 main roots, placing the tissue culture bottle in an indoor non-sunlight direct irradiation place for hardening off for about 5-7 days, taking out the tissue culture seedling from the bottle after hardening off, lightly cleaning the residual culture medium of the root by flowing water, and then transplanting the tissue culture seedling into a seedling pot to form turf soil: perlite=4:1 is used as a transplanting matrix, root fixing water is poured sufficiently, and new roots and new leaves can grow after 10-15 days after transplanting, so that detoxified seedlings are obtained.
(9) Planting: 7 months in 2020, the "Qian Xiang No. 1" purple passion fruit detoxified seedling obtained in the laboratory of the Guizhou fruit tree science research is planted in the female parent garden of Zhen Feng county Lu Rongxiang in Qian, southwest.
(10) And (3) field management: the greenhouse frame type frame shape is adopted, 10kg of farmyard manure and 0.5kg of compound fertilizer are applied to each plant, and the passion fruit is a waterlogged-afraid tree species, so that the soil is kept moist.
Conditions of culture: the culture temperature is 25+/-2 ℃, the illumination intensity is 2 000lx, the illumination time is 12h/d, and the relative humidity is 85-90%.
The composition of the induction medium includes; MS is taken as a basic culture medium, 4.74g/LMS powder, 30g/L sucrose, 7g/L agar, 1.0mg/L6-BA and 0.1mg/LNAA are added, and the pH value is regulated to 6.0.
The composition of the proliferation medium includes: MS is taken as a basic culture medium, 4.74g/LMS powder, 30g/L sucrose, 7g/L agar, 1.5mg/L6-BA and 0.1mg/LIBA are added, and the pH value is regulated to 6.0.
The rooting medium comprises the following components: taking 1/2MS as basic culture medium, adding 2.47g/L MS powder, 30g/L sucrose, 7g/L agar, 0.1mg/LIBA, and adjusting pH to 6.0.
The minimal medium used in this example is as follows:
TABLE 1 minimal Medium formulation
The Chinese name of BA is 6-benzylaminoadenine, and the chemical formula is C12H11N5.
NAA Chinese name is naphthalene acetic acid, and chemical formula is C12H10O2.
IBA has the chemical name of indolebutyric acid and the chemical formula of C12H12NO2.
The results of PCR are shown in the figure to explain the effect of secondary detoxification in detail
According to fig. 1-2, it can be seen that after the first detoxification, the TeMV and PLV are not completely removed, especially the PLV, but after the second detoxification, all three viruses are removed, the removal rate reaches 100%, which indicates that the detoxified seedling after the second detoxification can be used as a female parent for seedling cultivation.
TABLE 2 RT-PCR detection specific primers for three viruses
In the month 2 of 2020, 10 leaves are randomly taken and sent to the national academy of tropical agriculture institute biotechnology institute for analysis and detection, wherein the test mode is that the sample leaves are subjected to RNA extraction and reverse transcription to synthesize cDNA as a template for detecting the nucleotide sequence of a target virus; and respectively carrying out PCR amplification and sequencing identification on the passion fruit suspected virus sample cDNA through the designed conserved primers.
Claims (6)
1. The method for detoxification cultivation of passion fruit stem tips of purple fruits is characterized by comprising the following steps:
(1) Axillary bud disinfection treatment: immersing the passion fruit axillary buds of the passion fruit with 75% ethanol for 30s, washing with sterile water for 2 times, washing for 2min each time, and then using HgCl with the mass percentage of 0.1% 2 Sterilizing the solution for 15min, and then sufficiently cleaning with sterile water;
(2) Axillary bud culture: the axillary buds after disinfection are subjected to water absorption and proliferation culture by using MS+2.0mg/L6-BA and 0.1mg/L NAA culture medium to obtain strong axillary buds;
(3) Peeling off the stem tip: cutting tender tips of the newly grown axillary buds, sterilizing in the step (1), and peeling off the stem tips of 0.6 mm;
(4) Induction culture: inoculating the stripped stem tip into an induction culture medium for induction culture, wherein after 30 days of culture, a cluster of green cluster-shaped buds appear;
(5) Proliferation culture: cutting the stem tip into sections after the stem tip grows to 5-7 cm, and inoculating the sections with one internode at 0.4-0.6cm each to proliferation culture relay generation culture;
(6) Rooting culture: selecting proliferation buds with the length of 2-3 cm, cutting off tender buds from a basal part, and inoculating the tender buds into a rooting culture medium for rooting culture;
(7) Secondary stem tip detoxification culture: taking a robust test tube tissue culture seedling, cutting off a stem section with a terminal bud about 3cm, and culturing according to the steps (3) - (6);
(8) Hardening and transplanting: when the root length of the tissue culture seedling is 2-3 cm, selecting the tissue culture seedling with the same growth vigor, the plant height of 5-7 cm and 4-5 main roots, placing the tissue culture bottle at the indoor non-sunlight direct irradiation position for hardening off for 5-7 days, taking out the residual culture medium of the root after hardening off, lightly cleaning the tissue culture seedling with flowing water, then transplanting the tissue culture seedling into a seedling pot, pouring enough rooting water, and growing new roots and new leaves after transplanting for 10-15 days to obtain the detoxified seedling.
2. The method for detoxification cultivation of passion fruit stem tip according to claim 1, wherein: the culture conditions are as follows: the culture temperature of the steps (4) - (7) is 25+/-2 ℃, the illumination intensity is 2 000lx, the illumination duration is 12h/d, and the relative humidity is 85-90%.
3. The method for detoxification cultivation of passion fruit stem tip according to claim 1, wherein: the composition of the induction culture medium is MS as a basic culture medium, wherein 4.74g/LMS powder, 30g/L sucrose, 7g/L agar, 1.0mg/L6-BA and 0.1mg/LNAA are added, and the pH value is regulated to 6.0.
4. The method for detoxification cultivation of passion fruit stem tip according to claim 1, wherein: the proliferation culture medium takes MS as a basic culture medium, 4.74g/L MS powder, 30g/L sucrose, 7g/L agar, 1.5mg/L6-BA and 0.1mg/LIBA are added, and the pH value is regulated to 6.0.
5. The method for detoxification cultivation of passion fruit stem tip according to claim 1, wherein: the rooting culture medium takes 1/2MS as a basic culture medium, wherein 2.47g/L MS powder, 30g/L sucrose, 7g/L agar and 0.1mg/LIBA are added, and the pH value is regulated to 6.0.
6. The method for detoxification cultivation of passion fruit stem tip according to claim 1, wherein: the seedling substrate is prepared by mixing turfy soil and perlite according to a mass ratio of 4:1.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117084171A (en) * | 2023-09-05 | 2023-11-21 | 广西壮族自治区中国科学院广西植物研究所 | Detoxification method of passion fruits |
| CN118000102A (en) * | 2024-04-10 | 2024-05-10 | 云南龙藏生物科技有限公司 | Ultralow-temperature detoxification method of passion fruits and rapid propagation method of detoxified seedlings |
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2023
- 2023-03-17 CN CN202310263338.5A patent/CN116868891A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117084171A (en) * | 2023-09-05 | 2023-11-21 | 广西壮族自治区中国科学院广西植物研究所 | Detoxification method of passion fruits |
| CN117084171B (en) * | 2023-09-05 | 2024-05-03 | 广西壮族自治区中国科学院广西植物研究所 | Detoxification method of passion fruits |
| CN118000102A (en) * | 2024-04-10 | 2024-05-10 | 云南龙藏生物科技有限公司 | Ultralow-temperature detoxification method of passion fruits and rapid propagation method of detoxified seedlings |
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