CN101015656A - Method for preparation of pure gastrodia elata capsule with sleeping improving and hypoxia resistant functions - Google Patents

Abstract

The invention belongs to the method for preparing Gastrodia elata capsule for improving seep and anti-anoxia. The method comprises selecting Gastrodia elata in southwest of China such as Yunnan, Sichuan, and Guizhou province; squeezing to obtain juice and residue; soaking the residue in water, and squeezing to obtain juice and residue; extracting the residue with ethanol under reflux twice, recovering ethanol to obtain concentrated solution; mixing the two juices with concentrated solution, and spray-drying to obtain Gastrodia elata powder; and encapsulating, and packaging. The invention is to provide a method for preparing natural Gastrodia elata capsule with sleep improving and anti-anoxia effects. The inventive method can remarkably improve the yield of Gastrodia elata powder and Gastrodin, and can prepare Gastrodia elata health product with sleep improving and anti-anoxia effects.

Description

Can improve the manufacture method of the pure gastrodia capsule of sleep, anoxia enduring

Technical field

The invention belongs to a kind of manufacture method that can improve the pure gastrodia capsule of sleep, anoxia enduring.

Background technology

Rhizoma Gastrodiae is China's rare Chinese medicine, the successive dynasties book on Chinese herbal medicine is all classified as top grade, Rhizoma Gastrodiae is used existing more than 2,000 year history in China, it is only dizzy that modern pharmacology studies have shown that Rhizoma Gastrodiae has, control numb limbs and tense tendons, infantile convulsion, epilepsy clonus, tetanic effect, while is along with the continuous development of Chinese national economy, China's health product consumption demand is in period of expansion, the people is after solving the basic living problems, no longer satisfy simple requirements to life, begin to note the raising of quality of life, especially pay close attention to the medical treatment relevant and the consumption of health care with life and health, along with China's natural growth of the population and aged tendency of population, particularly the progressively raising of living standards of the people and health perception strengthen, China's health-product market will develop and enlarge rapidly, this provides the vast market space for the research and development of China's gastrodia tuber health product undoubtedly, and Ministry of Public Health has been classified Rhizoma Gastrodiae as the raw material list that can be used for health food, and this is to the health promoting product of exploitation based on Rhizoma Gastrodiae, development to China's public health work has important meaning.

The main component of Rhizoma Gastrodiae is gastrodine (Gastrodin), promptly to hydroxyl methanol～β～D glucopyranoside (P～Hydroxymethylphenyl～β～D～glucopyranoside), also be a higher main phenol glycoside composition of content in the Rhizoma Gastrodiae.In addition, also contain in the Rhizoma Gastrodiae: p-Hydroxybenzylalcohol (gastrodin unit) (p～Hy～droxybenzyl alcohol); Hydroxy benzaldehyde (p～Hdroxybenzaldehyde); 3,4～4-dihydroxy benzaldehyde (3,4～Di～hydroxybenzaldehyde); 4,4 '～the dihydroxy diphenyl methane (4,4 '～Dihydroxydiphenyl methane) etc.Except that above composition, also contain β～sitosterol, daucosterol, citric acid, Palmic acid, succinic acid etc. in the Rhizoma Gastrodiae tuber.Trace element is the highest with Fe content, and F, Mn, Zn, I take second place.Except above-mentioned micromolecule composition, also contain polysaccharide component and other unknown composition in the Rhizoma Gastrodiae.

At present, extraction to the Rhizoma Gastrodiae active ingredient, usually take drying Rhizoma Gastrodiae is extracted with decocting method, but this traditional extraction method, only can obtain 2～4% Rhizoma Gastrodiae powder yield (the dry powder weight/Rhizoma Gastrodiae fresh weight of extraction), the Rhizoma Gastrodiae powder yield is extremely low, and the extract that mainly contains the effective constituent gastrodine is still less, only account for usually extraction dry powder weight 0.10～0.11%.Rhizoma Gastrodiae powder yield and gastrodine extract low excessively caused the reduction of its curative effect, the waste of resource and the increase of cost.

Summary of the invention

The purpose of this invention is to provide a kind of manufacture method that can improve the pure gastrodia capsule of sleep, anoxia enduring, can significantly increase Rhizoma Gastrodiae powder yield and gastrodine extract with this method, and can make and have the tangible gastrodia tuber health product that improve sleep quality and resisting oxygen lack.

Method of the present invention is as follows:

(1) collection of raw material, in Yunnan, Sichuan, Guizhou and southwest, gather plantation be grown among 800～3800 meters remote, thickly forested mountains of height above sea level and on the unreclaimed virgin land, the Rhizoma Gastrodiae in 1～2 year of trophophase, acquisition time annual October～April next year;

(2) the fresh gastrodia elata piece of gathering is remained on ground temperature when gathering, in 4～10 hours, rejecting has the individual back of disease and pest to rinse well with tap water with the fresh gastrodia elata piece gathered, remove surperficial silt, be crushed to the tuber of particle diameter 0.5～1cm, use the juice extractor squeeze juice, get a squeezeding juice;

(3) residue after will squeezing the juice adds the clear water that accounts for residue weight 1/2, stirs, and leaves standstill 15～30 minutes, uses the juice extractor squeeze juice once more, secondary juice liquid;

(4) residue behind the secondary juice is added 80% ethanol that accounts for 2～4 times of weight of residue weight again, heat 50 ℃～60 ℃, reflux, extract, 2 times, each 2 hours, filter, get extracting solution, residue discards, extracting solution is heated 55 ℃～60 ℃, be concentrated into alcohol-free, concentrated solution;

(5) with first and second squeezeding juice and concentrated solution mix homogeneously, mixed liquor, again with mixed liquor with spray dryer with its spraying drying powder-forming, spray drying is instantaneous drying, inlet temperature is 180～200 ℃, 70～95 ℃ of outlet temperatures are made the Rhizoma Gastrodiae powder;

(6) semi-finished product are filled to capsule, loading amount is the 400mg/ grain;

(7) will get finished product after the capsule bottling/plate packing, finished product cobalt 60 irradiation sterilizations.(the requirement irradiation is even, dosage is accurate, irradiation average absorption amount is≤7.0KGY average absorbed dose inequality degree≤2.0) to arrive the sterilization purpose, guarantee that through the check of quality inspection portion salty pipe index, physical and chemical index and the microbiological indicator of product all meet national standard, after qualified, the finished product warehouse-in.

The health product that the inventive method is made are called " the pure gastrodia capsule of Fu Ruimai ", and clinical use result shows that following advantage is arranged:

1, manufacture method of the present invention can significantly increase Rhizoma Gastrodiae powder yield and gastrodine extract, and the Rhizoma Gastrodiae powder yield is 10～15%, gastrodine account for extraction dry powder weight 0.18～0.20%.The significantly increase of Rhizoma Gastrodiae powder yield and gastrodine extract causes the raising of its curative effect, the saving of resource and the reduction of cost.Especially the increase of other unknown composition extract in the Rhizoma Gastrodiae, using health product that the inventive method makes has dizzy, numb limbs and tense tendons, infantile convulsion, epilepsy clonus, the tetanic effect except that having the Rhizoma Gastrodiae traditional treatment, also has the effect that improves sleep quality and anoxia enduring.

2, the health product component made from the inventive method meets pharmaceutical control and administration and food law regulation, does not have chemical sweeting agent, and is nontoxic to human body.Show that through animal acute toxicity test underproof animal end sees any toxic reaction, its main organs does not have tangible change yet, and mice is 40g crude drug/kg to the maximum tolerated dose of medicine of the present invention, is 150 times of clinical people's consumption, and health product are safe and reliable.

3, the health product made from the inventive method need not decoct, and no bitterness sense is rich in the Rhizoma Gastrodiae active ingredient in the health product, also contain polysaccharide component and other unknown composition and various trace elements, are beneficial to absorption of human body, health invigorating, and taking convenience.

4, the present invention can make and have the tangible gastrodia tuber health product that improve sleep quality and resisting oxygen lack, and higher healing rehabilitation efficacy is arranged.The health product made from the inventive method have carried out following experiment through Nutrition and Food Safety Office of China Disease Prevention and control Centre:

(1) inspection item: improve sleep function～zoopery

Assay: with 0.6,1.2, the Fu Ruimai Rhizoma Gastrodiae capsule continuous irrigation stomach mice of 1.8/kgBW dosage 33 days, the result shows that each dosage group do not have influence to the mice body weight.Each dosage group can obviously shorten the inductive mice sleep of barbital sodium incubation period (P＜0.01), increase the inductive mice sleep incidence rate of sub-threshold dose barbital sodium (P＜0.05, P＜0.01), prolong the pentobarbital sodium inducing mouse length of one's sleep (P＜0.01), this shows that Fu Ruimai Rhizoma Gastrodiae capsule and pentobarbital sodium, barbital sodium have the effect of collaborative sleep.

(2) inspection item: anoxia tolerance function～zoopery

Assay: with 0.6,1.2, the Fu Ruimai Rhizoma Gastrodiae capsule continuous irrigation stomach mice of 1.8/kgBW dosage 32 days, the mice body weight is not had influence.Each dosage can improve anoxybiotic ability (P＜0.01) under the mice tolerance normal pressure, prolong the anoxia tolerance time (P＜0.01) that sodium nitrite poisons and causes, improve the mice tolerance anoxybiotic ability of acute cerebral ischemia (P＜0.01), this shows that Fu Ruimai Rhizoma Gastrodiae capsule has the effect of the anoxia in mice of raising tolerance.

Above-mentioned experiment shows that the health product made from the inventive method have following effect:

1, improve the sleep quality effect: the health product made from the inventive method can resist central excitation due to the animal caffeine and prolong the length of one's sleep of sodium pentobarbital, with sodium pentobarbital, chloral hydrate and pentothal sodium synergism is arranged all, the sedation of the health product made from the inventive method may be relevant with its reduction central nervous system's irritability.

2, resisting oxygen lack: the health product of day making with the inventive method to the normobaric hypoxia mice at tolerance, obviously prolong the death time, also remarkable to tolerance hypobaric hypoxia ability, in addition, can also significant prolongation inject isoproterenol and isoproterenol in advance and add the mice time-to-live in the Rhizoma Gastrodiae injection low pressure storehouse, this shows is organizing under the situation that oxygen consumption increases or anoxia endurance reduces, and the health product made from the inventive method all can improve body to anoxybiotic tolerance.

3, analgesic activity: with electric shock Mus tail method, the health product that proof is made with the inventive method all have very significant analgesia role, but analgesic effect less than morphine, the mouse subcutaneous injection gastrodia tuber preparation can resist obviously that lumbar injection acetic acid causes turns round the body reflection, proved also that with hot plate method improving the threshold of pain acts on, better to improving the vascular headache effect, treatment neurasthenia, vascular headache and depressive neurosis also there are effect preferably.

4, immunity function: be rich in the effect that polysaccharide has enhancing body nonspecific immunity and cellular immunization in the health product made from the inventive method, experiment shows that also the health product made from the inventive method have the mouse thymus of increasing weight, improve the Turnover of Mouse Peritoneal Macrophages phagocytic function, strengthen the effect of mice graft versus host disease.

It is of the present invention that to also have following advantage be to be raw material with the fresh gastrodia elata, but adopt the science preparation technology of process-cycle weak point, no environment and contamination of products large-scale industrial production, make the nutrition of Rhizoma Gastrodiae and medicinal ingredient is complete, gastrodin content is high, heavy metal etc. is polluted in the no course of processing Rhizoma Gastrodiae powder, can solve traditional Rhizoma Gastrodiae course of processing time long, make that the loss of Rhizoma Gastrodiae chemical constituent is many, active constituent content is low and problem such as medicinal difference.

The specific embodiment

(1) collecting region of raw material is Guizhou, gather plantation be grown among 1800 meters remote, thickly forested mountains of height above sea level and on the unreclaimed virgin land, the Rhizoma Gastrodiae 100kg in 1.5 years of trophophase, the annual December of acquisition time; Surface temperature is 3 ℃ during collection.

(2) with 3 ℃ of insulations of fresh gastrodia elata piece of gathering, with the fresh gastrodia elata piece gathered in 5 hours, reject the individuality of disease and pest is arranged after, rinse surperficial silt well with tap water, use the juice extractor squeeze juice, must a squeezeding juice;

(3) residue after will squeezing the juice adds the clear water that accounts for residue weight 1/2, stirs, and leaves standstill 20 minutes, uses the juice extractor squeeze juice once more, secondary juice liquid;

(4) residue behind the secondary juice is added 80% ethanol that accounts for 3 times of weight of residue weight again, heats 58 ℃, reflux, extract, 2 times, each 2 hours, filter, extracting solution, residue discards, and extracting solution is heated 55 ℃, be concentrated into alcohol-free, concentrated solution;

(5) with first and second squeezeding juice and concentrated solution mix homogeneously, mixed liquor, again with mixed liquor with spray dryer with its spraying drying powder-forming, spray drying is instantaneous drying, inlet temperature is 180～200 ℃, and 70～95 ℃ of outlet temperatures are made the about 12kg of Rhizoma Gastrodiae powder;

(6) semi-finished product are filled to capsule, loading amount is the 400mg/ grain;

(7) will get finished product after the capsule bottling/plate packing, finished product cobalt 60 irradiation sterilizations.(the requirement irradiation is even, dosage is accurate for finished product cobalt 60 irradiation sterilizations, irradiation average absorption amount is≤7.0KGY, average absorbed dose inequality degree≤2.0) to arrive the sterilization purpose, guarantee that through the check of quality inspection portion supervision index, physical and chemical index and the microbiological indicator of product all meet national standard, after qualified, the finished product warehouse-in.Instructions about how to take medicine are every day 3 times, and each 3, warm water takes.

In a word, the present invention can improve sleep, and the manufacture method of the pure gastrodia capsule of anoxia enduring can significantly increase Rhizoma Gastrodiae powder yield and gastrodine extract with this method, and can make the gastrodia tuber health product with tangible improvement sleep and resisting oxygen lack.

Claims (1)

1, a kind of can the improvement slept, and the manufacture method of the pure gastrodia capsule of anoxia enduring is characterized in that:

(1) collection of raw material in Yunnan, Sichuan, Guizhou and southwest, gathers that plantation is grown among 800～3800 meters remote, thickly forested mountains of height above sea level and on the unreclaimed virgin land, the Rhizoma Gastrodiae in 1～2 year of trophophase, acquisition time annual October～April next year;

(2) the fresh gastrodia elata piece of gathering is remained on ground temperature when gathering, in 4～10 hours, rejecting has the individual back of disease and pest to rinse well with tap water with the fresh gastrodia elata piece gathered, remove surperficial silt, be crushed to the tuber of particle diameter 0.5～1cm, use the juice extractor squeeze juice, get a squeezeding juice;

(3) residue after will squeezing the juice adds the clear water that accounts for residue weight 1/2, stirs, and leaves standstill 15～30 minutes, uses the juice extractor squeeze juice once more, secondary juice liquid;

(4) residue behind the secondary juice is added 80% ethanol that accounts for 2～4 times of weight of residue weight again, heat 50 ℃～60 ℃, reflux, extract, 2 times, each 2 hours, filter, get extracting solution, residue discards, extracting solution is heated 55 ℃～60 ℃, be concentrated into alcohol-free, concentrated solution;

(5) with first and second squeezeding juice and concentrated solution mix homogeneously, mixed liquor, again with mixed liquor with spray dryer with its spraying drying powder-forming, spray drying is instantaneous drying, inlet temperature is 180～200 ℃, 70～95 ℃ of outlet temperatures are made the Rhizoma Gastrodiae powder;

(6) semi-finished product are filled to capsule, loading amount is the 400mg/ grain;

(7) will get finished product after the capsule bottling/plate packing, finished product cobalt 60 irradiation sterilizations.