CN101011023A - Method for synchronous improvement of hybrid rape parents - Google Patents
Method for synchronous improvement of hybrid rape parents Download PDFInfo
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- CN101011023A CN101011023A CN 200710051475 CN200710051475A CN101011023A CN 101011023 A CN101011023 A CN 101011023A CN 200710051475 CN200710051475 CN 200710051475 CN 200710051475 A CN200710051475 A CN 200710051475A CN 101011023 A CN101011023 A CN 101011023A
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Abstract
The invention relates to a method for synchronously improving the parents of hybrid rape, based on present advanced parents and two selected parents to be transferred hidden corn sterility system and dominant sterility system, while the invention uses two sterility systems to process alternative selection that selecting parental sample, building base group, and selecting. The invention can hold the genetic heterogeneity of parents, and collect more useful gene points and alleles in two groups, to improve the advantages of parents. The invention provides a method based on gene heterogeneity molecule check and special match force analysis based on present advanced parents to build two turned selected base groups of parental samples, to improve the selection effect.
Description
Technical field
The invention belongs to rape variety seed selection field, relate to a kind of method of synchronous improvement of hybrid rape parents.
Background technology
Heterosis utilization is the effective technical way that improves crop yield, strengthens resistance and alleviation quality and output contradiction, is the key areas of crop genetic improvement.Parent's general performance and hereditary difference are heterotic bases, and more excellent gene of polymerization and allelomorph and coordinate interaction relationship between gene and between allelomorph among the parents are the main directions of genetic improvement always.The parent improves method commonly used two classes, one class is the proterties at qualitative character or major gene resistance control, employing is choosing, radiation, chemistry or space flight mutagenesis, transgenosis and hybridization, back cross breeding etc., but only effective to improvement indivedual or the minority proterties, be difficult to quantitative characters such as effective polymeric output, resistance and quality; Another kind of is the optimization polymerization of a plurality of proterties, at quantitative character, adopts population improvement technology such as recurrent selection.Recurrent selection is by periodic genetic recombination, selection and evaluation, important economy such as output, anti-patience and economical character are carried out comprehensive improvement, can effectively integrate the beneficial gene or the allelomorph that are distributed in different germ plasm resources, thereby improve the ratio of beneficial gene in the colony, break intergenic chain, increase the chance of beneficial gene reorganization, in the time of improvement colony, keep genetic diversity more widely.Recurrent selection is as the technology of population improvement, use quite extensive, lasting improvement to quantitative characters such as output, anti-patience, oil content, protein all obtains good effect, and the model of success is all arranged in cross pollinated plant, normal cross pollinated plant and the intragroup recurrent selection of self pollination crop.Heterotic utilization had both paid attention to improving parent's general performance (general combining ability), more will improve the specific combining ability of hybrid strain.Carry out the mutual recurrent selection of parents and can improve specific combining ability and general combining ability simultaneously, can be in parents colony polymerization more desirable genes site and allelomorph, and between the effective coordination gene and the interaction relationship between allelomorph.Therefore, the developing direction of recurrent selection breeding is mutual recurrent selection.But, for flower and normal cross pollinated plant certainly, only be confined to the improvement of single colony at present, mainly adopt male sterile line to solve the artificial difficulty of handing over mutually, still lack otherwise effective technique so far and carry out mutual polling improvement between parental population.
Summary of the invention
The present invention is based on existing strong advantage combination parents, according to variety source genetic diversity and specific combining ability analysis result, screen two parent's samples, and transformation is recessive gms line and dominant genic male sterility respectively, utilize two sterile systems to carry out mutual recurrent selection, realize continuing and improvement synchronously of hybrid rape parents, when improving parent's general combining ability, improve the specific combining ability between the parent.
The present invention is achieved through the following technical solutions: based on existing strong advantage combination parents, screen two parent's samples and respectively transformation be recessive gms line and dominant genic male sterility, utilize two sterile systems to carry out mutual recurrent selection, specifically comprise: the screening of parent's sample, the structure of basic population, mutual three steps of recurrent selection.
1. the screening of parent's sample
Selection has all kinds of variety sources of the excellent proterties of expectation and the biparent cross of existing strong advantage combination, analyze the specific combining ability that compares candidate parent and existing advantage combination parents, adopt molecular marking technique to carry out analysis of genetic diversity simultaneously, comprehensive two analysis results, two parent's samples that screening and former parents get together.Its concrete steps are as follows:
(1) according to breeding objective, Preliminary screening has the rape variety resource of expectation merit;
(2) specific combining ability analysis: rape variety that obtains through step (1) or combination are made up biparent cross with existing strong advantage, plantation F1 hybrid and parents, investigate phenophase, degeneration-resistant, economic characters and specific combining ability, carry out cluster analysis according to integral economic trait phenotype and specific combining ability result;
(3) molecular genetic diversity analysis: through rape variety or the combination that step (1) obtains, extract DNA, screen SSR (Simple sequence repeats, simple sequence repeats) primer, detect the SSR mark banding pattern of each kind, analyze cluster;
(4) comprehensive step (2) and step (3) cluster result are selected the kind sample of getting together with existing strong advantage combination parents, and one is male sterile line parent sample (SP), and another is parent's sample (RP) for recovering.
2. the structure of basic population
1) structure of recessive cytoblast sterile basic population
By hybridization, selfing with backcross, be recessive gms line with the transformation of male sterile line parent sample, then from respectively being to get the equivalent seed to mix, 1 generation of open pollination under the isolation condition, make up recessive cytoblast sterile recurrent selection basic population (RC0), its concrete steps are as follows:
(1) with each kind or combination among the SP and arbitrary preferred recessive gms line hybridization, F1 selfing;
(2) select sterile strain to backcross the BCF1 selfing with corresponding parent in step (1) the gained F2 colony;
(3) isolation condition is sowed and is planted step (2) gained BCF2, and the florescence is therefrom selected sterile strain, and the mark of listing is collected sterile strain after the maturation, and every is the 5-10 strain, respectively gets the equivalent seed and mixes, and gets the equivalent seed from every system again and mixes;
(4) step (3) gained population mixture open pollination generation under the isolation condition, the florescence is selected sterile strain, collects sterile strain after the maturation, the two low individual plants of screening, mixed in equal amounts makes up the RC0 basic population.
2) structure of dominant genic male sterile basic population
By hybridizing and backcrossing, to recover is that parent's sample RP changes into dominant genic male sterility, then from respectively being to get the equivalent seed to mix, the open pollination for 1 generation under the isolation condition, choose sterile strain, the two low individual plants of screening are got the equivalent seed and are mixed structure dominant genic male sterile recurrent selection basic population (DC0), and its concrete steps are as follows:
(1) with each kind among the RP and two low dominant genic male sterility hybridization (comprising heterozygous male sterile line and homozygous male sterile line);
(2) select sterile strain to backcross with corresponding parent (, showing that the parent is the recovery system of dominant genic male sterile, therefrom roguing selfing) in the F1 colony if the whole individual plants of F1 can be educated;
(3) the every system of step (2) gets equivalent (selfing) seed of backcrossing and mixes, 1 generation of open pollination under the isolation condition, choose sterile strain, and the two low individual plants of screening, mixed in equal amounts makes up the DC0 basic population.
3. mutual recurrent selection techniqueflow
RC0 and DC0 carry out mutual recurrent selection, improve two parental populations synchronously, and its concrete steps are as follows:
(1) under the isolation condition, plant two colonies respectively, choose sterile strain from the field according to breeding objective, every 600-1000 of colony strain, indoor species test and attributional analysis, from the two low high-oil offtake individual plant 200-300 strains of each mass screening, respectively get and equate that an amount of seed mixes and constitute from the test cross male parent MPR of recessive cytoblast sterile colony and test cross male parent MPD from dominant genic male sterile colony;
(2) get an amount of seed preparation combination in the selected sterile strain of each colony, remaining seed is properly preserved.Under the isolation condition, the selected individual plant of recessive cytoblast sterile colony is got an amount of seed and is planted (final singling 40-50 strain) work female parent in proper order, 2 strains of being separated by are plantation 2 row male parent MPD, florescence is pulled out the educated strain in the maternal row, the maternal row of maturing stage results seed, every strain equivalent seed mixing is closed as the sterile strain of correspondence institute combo; Simultaneously, under another isolation condition, the selected individual plant of dominant genic male sterile colony is got an amount of seed and is planted (final singling 40-50 strain) work female parent in proper order, every 2 strain systems plantation, 2 row male parent MPR, florescence is pulled out the educated strain in the maternal row, the maternal row of maturing stage results seed, every strain equivalent seed mixing is closed as the sterile strain of correspondence institute combo, gathers in the crops 400-600 combination;
(3) output, anti-patience and the quality of the combination of step (2) gained are identified, according to general performance, from recessive cytoblast sterile group and 30-40 the combination of final election respectively of dominant genic male sterile group;
(4) the corresponding sterile strain kind subclassification mixed in equal amounts of step (3) final election combination, the open pollination for 1 generation under the isolation condition makes up two basic populations that a new round is returned respectively;
(5) continue repeating step (1), (2), (3) and (4), simultaneously, screening excellent strain is to select or microspores culture according to pedigree, cultivates inbred line or male sterile line, i.e. Gai Liang parent.
Advantage of the present invention:
(1) adopts two sterile systems, solved an artificial difficult problem of handing over mutually in the polling process, improved test cross combo efficient, the real scheme of the mutual recurrent selection of a kind of cabbage type rape hybrid strain is provided, filled up the technological gap of the mutual recurrent selection of cabbage type rape.
(2) this technology keeps the multifarious while of amphilepsis, and polymerization more desirable genes site and allelomorph in two colonies are realized parents' lasting improvement, have both improved general combining ability, improve specific combining ability again.
(3) this technology proposes on existing strong advantage combination parents' basis, and by genetic diversity Molecular Detection and specific combining ability analysis, final election makes up parent's sample of two polling basic populations, can further improve the effect of polling.
Embodiment
The present invention is described in detail below in conjunction with embodiment.
Embodiment 1
1. the screening of parent's sample
G5034 is two low recessive gms lines, and R210 is an a pair of low-quality sterile restoring system, and both institute's combos are closed has strong advantage.Select candidate parent and G5034 and R210 hybridization, analyze specific combining ability, the candidate parent is divided into two groups.Simultaneously, adopt molecular marking technique, analyze each candidate parent's SSR banding pattern, two parent's samples that screening and G5034 and R210 get together.Comprehensive analysis of molecules and specific combining ability analysis result, determine to participate in two parent's samples that basic population makes up: male sterile line parent sample (SP) and recovery are parent's sample (RP);
2. the structure of basic population
(1) structure of recessive gms line basic population: each parent of SP sample and G5034 hybridization, the F1 selfing selects sterile strain to backcross the BC1F1 selfing with corresponding parent in the F2 colony.The isolation condition is sowed and is planted BCF2, florescence marks sterile strain, collect sterile strain after the maturation, every is the 5-10 strain, respectively gets the equivalent seed and mixes, and gets the equivalent seed from every system again and mixes, the open pollination for 1 generation under the isolation condition, collect sterile strain, the two low sterile strain 30-40 strains of screening are got the equivalent seed and are mixed structure recessive cytoblast sterile basic population (RC0).
(2) structure of dominant genic male sterility basic population: each parent of RP sample and D5008 (two low heterozygous dominant genic male sterility) hybridization, selecting the sterile strain of F1 to backcross with corresponding parent (is the recovery system of dominant genic male sterile as the parent, then F1 selfing), every system fetches and hands over or the mixing of selfing equivalent seed, the open pollination for 1 generation collected sterile strain seed, behind the attributional analysis under the isolation condition, screening 30-40 strain is got the equivalent seed and is mixed structure dominant genic male sterile basic population (DC0).
3. mutual recurrent selection techniqueflow
(1) isolation condition is sowed and is planted two basic populations, and the florescence marks sterile strain, and the maturing stage is gathered in the crops the sterile preferably individual plant of comprehensive The Characters, and each colony respectively selects the 600-1000 strain, and through indoor species test, attributional analysis, each screens the 200-300 strain, enters test cross and identifies.
(2) each selected sterile individual plant of each colony is divided into 3 parts, 1 part of preservation, and 1 part of branch colony type hybrid is made test male parent: MPR (recessive cytoblast sterile) and MPD (dominant genic male sterile), does to test maternal for 1 part.Under the isolation condition, recessive cytoblast sterile strain and MPD preparation combination, and 400-600 combination prepared in dominant genic male sterile strain and MPR preparation combination under another isolation condition altogether.
(3) combination is observed and is identified.Under the condition of unanimity, evaluation is observed in each combination, investigate yield traits, degeneration-resistant proterties and quality etc., each final election goes out 30-40 combination, corresponding sterile individual plant is got the equivalent seed and is mixed, and open the pollination for 1 generation under the isolation condition synthesized the basic population of new round choosing respectively.
Repeat above (1), (2) and (3) three steps, each takes turns that selected sterile strain enters is that choosing or Isolated microspore are cultivated, and cultivating male sterile line and recovering is promptly to obtain the parents that improve.
Embodiment 2
1. the screening of parent's sample
With embodiment 1.
2. the structure of basic population
(1) structure of recessive cytoblast sterile basic population: with embodiment 1.
(2) structure of dominant genic male sterile basic population: each parent of RP sample and D5226 (two low homozygous dominant genic male sterility) hybridization, select sterile strain of F1 (parent is temporary maintainer line) and corresponding parent to backcross and maybe can educate strain (parent is for recovering system) selfing, every system fetches and hands over or the mixing of selfing equivalent seed, the open pollination for 1 generation under the isolation condition, collect sterile strain seed, behind the attributional analysis, screening 30-40 strain is got the equivalent seed and is mixed structure dominant genic male sterile basic population (DC0).
3. mutual recurrent selection techniqueflow
With embodiment 1.
Claims (5)
1. the method for a synchronous improvement of hybrid rape parents, it is characterized in that: based on existing strong advantage combination parents, screen two parent's samples and respectively transformation be recessive gms line and dominant genic male sterility, utilize two sterile systems to carry out mutual recurrent selection, comprising: the screening of parent's sample, the structure of basic population, mutual three steps of recurrent selection.
2. according to the method for right 1 described synchronous improvement of hybrid rape parents, it is characterized in that: select to have all kinds of variety sources of the excellent proterties of expectation and the biparent cross of existing strong advantage combination, analyze the specific combining ability that compares candidate parent and existing advantage combination parents, adopt molecular marking technique to carry out analysis of genetic diversity simultaneously, comprehensive two analysis results, two parent's samples that screening and former parents get together, concrete steps are as follows:
(1) according to breeding objective, Preliminary screening has the rape variety resource of expectation merit;
(2) specific combining ability analysis: rape variety that obtains through step (1) or combination are made up biparent cross with existing strong advantage, plantation F1 hybrid and parents, investigate phenophase, degeneration-resistant, economic characters and specific combining ability, carry out cluster analysis according to integral economic trait phenotype and specific combining ability result;
(3) molecular genetic diversity analysis: through rape variety or the combination that step (1) obtains, extract DNA, the screening simple sequence repeats the SSR primer, detects the SSR mark banding pattern of each kind, analyzes cluster;
(4) comprehensive step (2) and step (3) cluster result, final election makes up the kind sample that parents get together with existing strong advantage, and one is male sterile line parent sample SP, and another is parent's sample RP for recovering.
3. according to the method for the described synchronous improvement of hybrid rape parents of claim 1, it is characterized in that: by hybridization, selfing with backcross, with male sterile line parent sample SP transformation is recessive gms line, then from respectively being to get the equivalent seed to mix, the open pollination for 1 generation under the isolation condition, make up recessive cytoblast sterile recurrent selection basic population RC0, its concrete steps are as follows:
(1) with each kind or combination among the SP and arbitrary preferred recessive gms line hybridization, F1 selfing;
(2) select sterile strain to backcross the BCF1 selfing with corresponding parent in step (1) the gained F2 colony;
(3) isolation condition is sowed and is planted step (2) gained BCF2, and the florescence is therefrom selected sterile strain, and the mark of listing is collected sterile strain seed after the maturation, and every is the 5-10 strain, respectively gets the equivalent seed and mixes, and gets the equivalent seed from every system again and mixes;
(4) step (3) gained population mixture open pollination generation under the isolation condition, the florescence is selected sterile strain, collects sterile strain after the maturation, and the two low individual plants of screening are got the mixing of equivalent seed and are made up the RC0 basic population.
4. according to the method for right 1 described synchronous improvement of hybrid rape parents, it is characterized in that: by hybridizing and backcrossing, to recover is that parent's sample RP changes into dominant genic male sterility, then from respectively being to get the equivalent seed to mix, the open pollination for 1 generation chosen sterile strain under the isolation condition, the two low individual plants of screening, get the equivalent seed and mix structure dominant genic male sterile recurrent selection basic population DC0, its concrete steps are as follows:
(1) with each kind among the RP and two low dominant genic male sterility hybridization, comprises heterozygous male sterile line and homozygous male sterile line;
(2) select sterile strain to backcross in the F1 colony,, show that the parent is the recovery system of dominant genic male sterile, therefrom roguing selfing if the whole individual plants of F1 can be educated with corresponding parent;
(3) get equivalent from the every system of step (2) gained and backcross and the selfed seed mixing, the open pollination for 1 generation chosen sterile strain under the isolation condition, screening pair low individual plants, mixed in equal amounts structure DC0 basic population.
5. according to the method for right 1,3 and 4 described synchronous improvement of hybrid rape parents, it is characterized in that: RC0 and DC0 carry out mutual recurrent selection, and its concrete steps are as follows:
(1) under the isolation condition, plant two colonies respectively, choose sterile strain from the field according to breeding objective, every 600-1000 of colony strain, indoor species test and attributional analysis, from the two low high-oil offtake individual plant 200-300 strains of each mass screening, respectively get and equate that an amount of seed mixes, constitute from the test cross male parent MPR of recessive cytoblast sterile with from the test cross male parent MPD of dominant genic male sterile colony;
(2) get an amount of seed preparation combination in the selected sterile strain of each colony, remaining seed is properly preserved.Under the isolation condition, the selected individual plant of recessive cytoblast sterile colony is got an amount of seed and is planted in proper order, final singling 40-50 strain is done maternal, 2 strains of being separated by are plantation 2 row male parent MPD, florescence is pulled out the educated strain in the maternal row, the maternal row of maturing stage results seed, every strain equivalent seed mixing is closed as the sterile strain of correspondence institute combo; Simultaneously, under the isolation condition, the selected individual plant of dominant genic male sterile colony is got an amount of seed and is planted 40-50 strain work female parent in proper order, every 2 strain systems plantation, 2 row male parent MPR, florescence is pulled out the educated strain in the maternal row, the maternal row of maturing stage results seed, every strain is got the mixing of equivalent seed and is closed as the sterile strain of correspondence institute combo, gathers in the crops 400-600 combination;
(3) output, anti-patience and the quality of the combination of step (2) gained are identified, according to general performance, from recessive cytoblast sterile group and 30-40 the combination of final election respectively of dominant genic male sterile group;
(4) the corresponding sterile strain kind subclassification mixed in equal amounts of step (3) final election combination, the open pollination for 1 generation makes up two basic populations of new round choosing;
(5) continue repeating step (1), (2), (3) and (4), simultaneously, screening excellent strain is to select or microspores culture according to pedigree, cultivates inbred line or male sterile line, i.e. Gai Liang parent.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102742496A (en) * | 2012-03-27 | 2012-10-24 | 贵州省油菜研究所 | Rapid transfer improvement method for rape recessive homozygosis sterile line |
| CN104823836A (en) * | 2015-05-04 | 2015-08-12 | 河北省农林科学院粮油作物研究所 | Method for obtaining Jidou12 backcrossing introgression population |
| CN108416189A (en) * | 2018-02-08 | 2018-08-17 | 北京市农林科学院 | A kind of variety of crops Heterosis identification method based on molecular marking technique |
| CN109566399A (en) * | 2018-12-26 | 2019-04-05 | 华中农业大学 | A kind of method of S haplotype molecular marking supplementary breeding rape of self incompatible lin |
| CN111771716A (en) * | 2020-07-24 | 2020-10-16 | 江西省农业科学院作物研究所 | Crop genetic breeding method for efficiently utilizing heterosis |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1127893C (en) * | 1999-06-04 | 2003-11-19 | 华中农业大学 | Two-line male sterile method for assortively breeding ecological-type cytoplasm of rape |
-
2007
- 2007-01-31 CN CN2007100514753A patent/CN101011023B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102742496A (en) * | 2012-03-27 | 2012-10-24 | 贵州省油菜研究所 | Rapid transfer improvement method for rape recessive homozygosis sterile line |
| CN102742496B (en) * | 2012-03-27 | 2014-08-06 | 贵州省油菜研究所 | Rapid transfer improvement method for rape recessive homozygosis sterile line |
| CN104823836A (en) * | 2015-05-04 | 2015-08-12 | 河北省农林科学院粮油作物研究所 | Method for obtaining Jidou12 backcrossing introgression population |
| CN108416189A (en) * | 2018-02-08 | 2018-08-17 | 北京市农林科学院 | A kind of variety of crops Heterosis identification method based on molecular marking technique |
| CN109566399A (en) * | 2018-12-26 | 2019-04-05 | 华中农业大学 | A kind of method of S haplotype molecular marking supplementary breeding rape of self incompatible lin |
| CN111771716A (en) * | 2020-07-24 | 2020-10-16 | 江西省农业科学院作物研究所 | Crop genetic breeding method for efficiently utilizing heterosis |
| CN111771716B (en) * | 2020-07-24 | 2022-03-04 | 江西省农业科学院作物研究所 | Crop genetic breeding method for efficiently utilizing heterosis |
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