CN102057862B - Application of upland cotton flower based red spot marked character - Google Patents
Application of upland cotton flower based red spot marked character Download PDFInfo
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- CN102057862B CN102057862B CN 201010506743 CN201010506743A CN102057862B CN 102057862 B CN102057862 B CN 102057862B CN 201010506743 CN201010506743 CN 201010506743 CN 201010506743 A CN201010506743 A CN 201010506743A CN 102057862 B CN102057862 B CN 102057862B
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Abstract
The invention relates to an application of the upland cotton flower based red spot marked character. In the application, a hybrid cotton parent or a pure line variety with the flower based red spot market character as a male parent or a female parent is used to hybridize other hybrid cotton parents or pure line varieties with or without the flower based red spot marked character to produce a hybrid variety; and after the produced hybrid variety is sawn, the identity and purity of the hybrid variety can be intuitively identified and inspected according to the proportion of the flower based red spot, wherein the hybrid cotton parent with the flower based red spot marked character comprises a two-line male sterile line, a three-line male sterile line, a maintenance line and a restoring line. By the application, the production quality of cotton seeds can be effectively controlled, the fake or poor purity seeds are prevented from impacting the market, and the technical support and protection are provided for production and sale of qualified seeds so as to maintain the lawful benefit of enterprises and peasants and promote healthy development of cotton planting industry. The application has high application value.
Description
Technical field
The present invention relates to the application method of upland cotton flower based red spot marked character, belong to the agricultural biotechnologies application.
Background technology
Cotton occupies critical role in China economic crops, sown areas of cotton reach more than 5,000,000 hectares throughout the year, plants cotton area and occupies the second place of the world, and total producing ranks first in the world; Cotton is also the primary raw material of China's textile industry, and textile exports is China's traditional advantage industry, relates to cotton industry chain length, employment group is large, and therefore, the supply security of cotton is to the sustainable development of agricultural and social harmony is stable has important practical significance.Along with the development of cotton breeding technology, high yield, high-quality, disease-resistant, transgenosis new varieties continue to bring out.Because cotton has the indeterminate growth habit, different cultivars is difficult to differentiation directly perceived, make and sell fake and inferior cotton seeds and the piracy of intellectual property rights event emerges in an endless stream, the harmful farming part of cheating the farmers constantly occurs, the interests of vast farmers have not only been injured, reduce the level of production, seriously restricted the sound development of Cotton seed industry, all caused heavy economic losses also for enterprise, peasant and country.
In China, except the Northwest inland cotton region has a small amount of sea-island cotton, other cotton region plantation be the Upland Cotton type, upland cotton accounts for China and plants more than 95% of cotton area.Therefore; seek out a kind of can be intuitively, the easily and fast and effective method of identification of species authenticity, specificity and purity; can effectively control the cotton seeds quality of production; avoid the seed of fake and forged and purity difference to raid market; provide technical support and protection for producing and selling registered seed; with maintaining enterprise and peasant's legitimate rights and interests, promote the sound development of Cotton seed industry.
Summary of the invention
The object of the invention is to use upland cotton flower based red spot marked character seed selection hybrid cotton parents and kind, production has crossbreed and conventional kind of this mark property, utilizes then that this mark property carries out intuitively, easily and fast and effectively the differential variety true and false and purity.
The application of upland cotton flower based red spot marked character of the present invention, it is to use to have the hybrid cotton parents of spending basic erythema mark property or pure line cultivar as male parent or female parent, spend other hybrid cotton parents and the pure line cultivar of basic erythema mark property to hybridize with having or do not have, produce crossbreed, the crossbreed that produces is after planting intuitively differentiated and the check crossbreed true and false and purity according to the ratio of spending basic erythema; Described have the hybrid cotton parents of spending basic erythema mark property and comprise that two-line sterile line, three is male sterile line, maintainer and restorer.
The crossbreed of described production has spends basic erythema mark property, and it shows as bud when in full bloom, and petal is milky, the red or purplish red color base spot of petal bottom tool, and spending basic erythema mark property is dominant inheritance.
The described hybrid cotton parents of basic erythema mark property of spending is: (1), introduce, collect have a upland cotton new germ plasm of spending basic erythema mark property; Or (2), have by what genetic improvement obtained the upland cotton new germ plasm of spending basic erythema mark property by the hybridization of upland cotton and wild cotton; Or (3), with (1) and/or (2) breeding method by conventional breeding method or combined mutagenesis, trans-exogenous gene, acquisition have a new hybrid cotton parents of upland cotton flower based red spot marked character.
The application of described upland cotton flower based red spot marked character, be to utilize to have to spend basic erythema mark property upland cotton pure line cultivar, by expanding propagation, produce conventional the kind, the routine kind that produces is after planting intuitively differentiated and the conventional true and false and the purity of planting of check according to the ratio of spending basic erythema.
The routine kind of described production has spends basic erythema mark property, and it shows as bud when in full bloom, and petal is milky, the red or purplish red color base spot of petal bottom tool.
The described pure line cultivar of basic erythema mark property of spending is: (1), introduce, collect have a upland cotton new germ plasm of spending basic erythema mark property; Or (2), have by what genetic improvement obtained the upland cotton new germ plasm of spending basic erythema mark property by the hybridization of upland cotton and wild cotton; Or (3), utilize (1) and/or (2) by the breeding method of conventional breeding method or combined mutagenesis, trans-exogenous gene, acquisition have a new pure line cultivar of upland cotton flower based red spot marked character.
The present invention uses upland cotton flower based red spot marked character seed selection hybrid cotton parents and kind, produces crossbreed and conventional kind the with this mark property, utilize this mark property can be intuitively, easily and fast and effectively the differential variety true and false and purity.The cotton seeds quality of production can be effectively controlled in this invention; avoid the seed of fake and forged and purity difference to raid market; provide technical support and protection for producing and selling registered seed, with maintaining enterprise and peasant's legitimate rights and interests, promote the sound development of Cotton seed industry.This invention has very high using value and important social effect.
Embodiment
Embodiment 1 and embodiment 2 are basic breeding material source and seed selection example.
Embodiment 1
Mutant: in July, 2002, chancing on a strain pattern in cotton breeding material garden is numbered 02107 strain is milky, the mutated individual of the red basic spot of petal bottom tool, 10 bells of bagging selfing at once, send Hainan to add the generation plantation in the seed of results September then, selfing bell plant type is not unanimously separated, and strain is isozygotied, and is namely BJ-7 surely;
Embodiment 2
Distant hybridization: in August, 2003, safflower base spot allotetraploid offspring H401 with upland cotton * wild cotton (Bi Keshi is cotton) distant hybridization, turn cry1Ab/cry1Ac and CpTI gene pest-resistant material SGK321 hybridization with normally spending in vain without basic spot, backcross once in Hainan the end of the year then, next year is at (the F of H401 * SGK321)
1In * SGK321 colony, separating a strain pattern is milky, (this is published in " the Bi Keshi cotton gradually is seeped into the genetic analysis of the flower color gene of upland cotton " research paper of " Acta Genetica Sinica " the 4th phase the 27th volume in 2000 with how to reflect star, Li Zengshu etc. of hereditary institute of the Chinese Academy of Sciences, academy of agricultural sciences, Shijiazhuang the individual plant of the red basic spot of petal bottom tool, result of study about " from the hybrid derivatives of G. hirsutum×G. bickii, isolating the milky white little basic spot pure lines of spending " is basically identical).This individual plant adds generation through generation selfing, Hainan, orientations is selected in F in 2006
5Shi Yucheng spends the pest-resistant pure lines of basic erythema, names to be BJ-18.
Embodiment 3～6 is the seed selection example of new hybrid cotton parents and pure line cultivar.
Embodiment 3
2003 to spend basic erythema marker material BJ-7 as maternal, take large bell, how anti-material 896 as male parent, add generation, directive breeding through generation selfing, Hainan, bred large bell, how anti-type hybrid cotton parents (pure line cultivar) BJ-7896 that tool is spent basic erythema mark property in 2006.
Embodiment 4
2006 take the neat 6S of two-line sterile line as maternal, to spend basic erythema marker material BJ-18 as male parent, choosing has the sterile strain of spending basic erythema mark for maternal in follow-up segregative generation respectively, be paternal hybrid with having the fertile plant of spending basic erythema mark, bred in 2009 and to have the neat BJ-618S of two-line sterile line hybrid cotton parents that spends basic erythema mark.
Embodiment 5
2005 is that the neat 5B of maintainer is as maternal take three respectively, to spend basic erythema marker material BJ-7 as paternal hybrid, offspring's purifying is from generation to generation constantly that the neat 5A of male sterile line is measured sterility with three, breeds that to have the hybrid cotton parents three of spending basic erythema mark be that male sterile line QBJ-5A and three is maintainer QBJ-5B in 2009;
Embodiment 6
2006 take the neat extensive 307R of restorer as maternal, to spend basic erythema marker material BJ-18 as paternal hybrid, offspring's purifying is from generation to generation constantly that the neat 5A of male sterile line is measured recovery rate with three, breeds in 2009 to have that to spend basic erythema mark hybrid cotton parents three be restorer QBJ718R.
Embodiment 7～8 is production and the application examples of crossbreed.
Embodiment 7
2008 take self-fertile normal without the large bell material of basic spot 23-1 as maternal, spend basic erythema marker material BJ-18 as male parent to have, artificial emasculation, pollination are dialled except male parent when pollination finishes, and produce mix 276 kilograms of 118 crossbreed of China.Send Hainan to identify the seed true and false and purity the seed sampling of results the end of the year then, plant altogether 500 strains, intuitively investigate pattern at full-bloom stage, having the plant of spending basic erythema mark property is 492 strains, account for 98.4% of sum, illustrating that the seeding farmer does not mix makes vacation or has participated in few other seed, judge that this crossbreed is pure dan, and purity is qualified.
Embodiment 8
Spent basic erythema marker material as maternal to have in 2008, and spent basic erythema marker material BJ-18 as male parent to have, artificial emasculation, pollination are dialled except male parent when pollination finishes, and produce 253 kilograms of assorted 618 crossbreed of China.Send Hainan to identify the true and false and purity the seed sampling of results the end of the year then, plant altogether 500 strains, intuitively investigate pattern at full-bloom stage, having the plant of spending basic erythema mark property is 499 strains, account for 99.8% of sum, illustrating that the seeding farmer does not mix makes vacation or has participated in few other seed, judge that this crossbreed is pure dan, and purity is qualified.
Embodiment 9 (conventional production and application examples of planting)
2003 to spend basic erythema marker material BJ-7 as maternal, take large bell, how anti-material 896 as male parent, add generation, directive breeding through generation selfing, Hainan, bred large bell, how anti-type hybrid cotton parents (pure line cultivar) BJ-7896 that tool is spent basic erythema mark property in 2006.
2008, with having the pure line cultivar BJ-7896 that spends basic erythema mark property, to concentrate in flakes, isolate and expand numerous 1 hectare, full-bloom stage is dialled except not having the hybrid strain of spending basic erythema mark, and the results routine is planted 1230 kilograms altogether.Send Hainan to identify the true and false and purity the seed sampling of results the end of the year then, plant altogether 500 strains, intuitively investigate pattern at full-bloom stage, having the plant of spending basic erythema mark property is 496 strains, account for 99.2% of sum, illustrate that expanding numerous peasant household does not mix and make vacation or participated in few other seed, judge that this routine kind is pure dan, and purity is qualified.
Claims (4)
1. the application of upland cotton flower based red spot marked character, it is characterized in that, use has the hybrid cotton parents of spending basic erythema mark property or pure line cultivar as male parent or female parent, spend other hybrid cotton parents and the pure line cultivar of basic erythema mark property to hybridize with having or do not have, produce crossbreed, the crossbreed that produces is after planting intuitively differentiated and the check crossbreed true and false and purity according to the ratio of spending basic erythema; Described have the hybrid cotton parents of spending basic erythema mark property and comprise that two-line sterile line, three is male sterile line, maintainer and restorer; The crossbreed of described production has spends basic erythema mark property, and it shows as bud when in full bloom, and petal is milky, the red or purplish red color base spot of petal bottom tool, and spending basic erythema mark property is dominant inheritance.
2. the application of upland cotton flower based red spot marked character claimed in claim 1, is characterized in that, the described hybrid cotton parents of basic erythema mark property of spending is: (1), introduce, collect have a upland cotton new germ plasm of spending basic erythema mark property; Or (2), have by what genetic improvement obtained the upland cotton new germ plasm of spending basic erythema mark property by the hybridization of upland cotton and wild cotton; Or (3), with (1) and/or (2) breeding method by conventional breeding method or combined mutagenesis, trans-exogenous gene, acquisition have a new hybrid cotton parents of upland cotton flower based red spot marked character.
3. the application of upland cotton flower based red spot marked character, is characterized in that, utilizes to have and spend basic erythema mark property upland cotton pure line cultivar, pass through expanding propagation, produce conventional the kind, the routine kind of generation is after planting intuitively differentiated and the conventional true and false and the purity of planting of check according to the ratio of spending basic erythema; The routine kind of described production has spends basic erythema mark property, and it shows as bud when in full bloom, and petal is milky, the red or purplish red color base spot of petal bottom tool, and spending basic erythema mark property is dominant inheritance.
4. the application of claim 1 or 3 described upland cotton flower based red spot marked character, is characterized in that, the described pure line cultivar of basic erythema mark property of spending is: (1), introduce, collect have a upland cotton new germ plasm of spending basic erythema mark property; Or (2), have by what genetic improvement obtained the upland cotton new germ plasm of spending basic erythema mark property by the hybridization of upland cotton and wild cotton; Or (3), utilize (1) and/or (2) by the breeding method of conventional breeding method or combined mutagenesis, trans-exogenous gene, acquisition have a new pure line cultivar of upland cotton flower based red spot marked character.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010506743 CN102057862B (en) | 2010-10-12 | 2010-10-12 | Application of upland cotton flower based red spot marked character |
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| CN 201010506743 CN102057862B (en) | 2010-10-12 | 2010-10-12 | Application of upland cotton flower based red spot marked character |
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| CN102057862A CN102057862A (en) | 2011-05-18 |
| CN102057862B true CN102057862B (en) | 2013-06-19 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112301143B (en) * | 2020-09-15 | 2022-09-13 | 河北省农林科学院棉花研究所（河北省农林科学院特种经济作物研究所） | Method for identifying floral erythema trait of Asian cotton by using KASP marker |
| CN115443904A (en) * | 2022-09-30 | 2022-12-09 | 石家庄市农林科学研究院 | Method for breeding conventional variety and identifying purity by using index character of Bikstan cotton |
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Non-Patent Citations (4)
| Title |
|---|
| 何鉴星等.比克氏棉渐渗到陆地棉的花色基因的遗传分析.《遗传学报》.2000,第27卷(第4期),第344-350页. * |
| 危家文等.杂交棉不去雄人工制种技术研究.《湖南文理学院学报》.2008,第20卷(第2期),第63-65页. * |
| 李俊兰等.远缘杂交创造棉花优异种质的研究初报.《河北农业科学》.2003,第7卷第150-151页. * |
| 黎鸿慧.棉花花器颜色的研究进展简述.《中国棉花学会2009年年会论文汇编》.2009,第72-73页. * |
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