CN101007049A - Application of nitric oxide donor and Chinese herbal preparation on nonspecific immune function of prawns - Google Patents
Application of nitric oxide donor and Chinese herbal preparation on nonspecific immune function of prawns Download PDFInfo
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- CN101007049A CN101007049A CN 200710026468 CN200710026468A CN101007049A CN 101007049 A CN101007049 A CN 101007049A CN 200710026468 CN200710026468 CN 200710026468 CN 200710026468 A CN200710026468 A CN 200710026468A CN 101007049 A CN101007049 A CN 101007049A
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Abstract
The invention relates to a kind of preparation that is made of donator of nitrogen monoxidum and Chinese herbal medicine. The preparation can substantially increase the non-specificity immunity of prawns and the prawn's ability to resist diseases so that it can be used in immune domain of preventing disorders in cultivating of prawns. The characteristic of the invention includes the following aspects: it is made of certain amount of donator of nitrogen monoxidum and Chinese herbal medicine and it can regulate the nonspecific immunity of prawns; the Chinese herbal medicine includes astragali radix and licorice root at a ratio of 3:1; the mentioned donator of nitrogen monoxidum is arginine; the ratio of the donator and Chinese herbal medicine is 1:5.
Description
Technical field:
The present invention relates to a kind of prawn immunostimulant, be applicable to shellfish, the increase of the immunologic function of animals such as shell-fish belongs to the immune formulation field, exactly is that nitric oxide donors and Chinese herbal and crude drugs preparations are to the nonspecific immunity of prawns purposes.
Background technology:
Along with the expansion of aquaculture scale, the raising of intensification degree and the deterioration of water quality, the generation of disease is also more and more frequent, and the extent of injury is also increasing, has a strong impact on health, the sustainable development of culture fishery.According to according to a preliminary estimate, the economic loss that caused because of disease in 2005 is up to more than several hundred million units.Again and again the disease problem of breaking out not only causes great economic loss to plant, has had a strong impact on the development of culture fishery, and has proposed a problem that presses for solution.How to prevent and treat aquatic animal disease?
But conventional medicine and method are difficult to control effectively to the great epidemic disease of aquatic animal, and breakthrough is not arranged so far, and the residual great attention that human food's potential safety hazard has been caused countries in the world government and consumer of medicine.By the immune defense system of immunological technique excitating organism self, improve the resistivity of body to various diseases, be one of valid approach of generally acknowledging at present, also meet the demand of people to " green " aquatic products.Therefore, when taking other prophylactico-therapeutic measuress, people attention diversion to how transferring or activate aquatic animal autoimmune system, improve its non-specific immunity and premunition, to reach the prevention and the purpose of resist the disease.
Nitric oxide (Nitric oxide NO) is a kind of newfound biological messenger molecule, effector molecule and immune modulatory molecules, its generation depend on nitricoxide synthase (Nitric oxide synthase, NOS).NO is distributed widely in each organ and tissue of organism, participates in the activity of the multiple important physical pathology of body, is the physiology courier of blood circulation, neurotransmission and immunologic cytotoxicity, is again the active medium under many pathological states.The nearest NO that studies show that has immunology meaning widely, and it is many-sided to the influence of body's immunological function, can be by non-specific antibacterial, fungus, parasite and virus etc., the nonspecific immunity of enhancing body of killing and wounding; Also can control the specific immunity of body; Can also regulate the panimmunity active medium, more the wide influence body's immunological function.Biological intravital NO is to be substrate by NOS with L-arginine and molecular oxygen, and one of arginic two guanidine radicals nitrogen of equal value of catalysis L-through 5 electronics oxidation reactions, produce L-citrulline and NO.
China has abundant Chinese herbal medicine resource, and application Chinese herb medicine among the people is prevented and treated aquatic animal disease long history.In (beast) research and fish production practice medically prove that all Chinese herbal medicine is particularly useful for intensification, the needs of scale production of current culture fishery as mixed feeding agent or feed additive, is convenient to carry out colony's control of cultured fishes disease.Use the herbal control fish diseases and not only can solve the residual problem such as exceed standard of anti-(anti-) property of medicine of initiation such as chemicals, antibiotic and cultured fishes medicine, and meet the disease control criterion of the nuisanceless aquatic products industry of development, production green aquatic product fully.In recent years, along with Chemistry for Chinese Traditional Medicine and Chinese medicine immunopharmacology progress of research, now proved 200 surplus kind of a Chinese herbal medicine many-sided immunologic active material is arranged, can influence and regulate immunity function of animal.
Summary of the invention:
The objective of the invention is to: develop a kind of preparation that strengthens nonspecific immunity of prawns, reduce the generation of disease in the prawn culturing process, can not cause drug residue again simultaneously aquatic products.
The technical solution adopted for the present invention to solve the technical problems is: based on Chinese herbal and crude drugs preparations, add a small amount of nitric oxide donors, thereby improve the non-specific immunity of prawn.The mixed proportion of Chinese herbal medicine is the Radix Astragali: Radix Glycyrrhizae=3: 1.
Feature of the present invention is summarized as: it is to mix by a certain percentage with nitric oxide donors and Chinese herbal and crude drugs preparations, can regulate nonspecific immunity of prawns; The composition of Chinese herbal and crude drugs preparations is the Radix Astragali and Radix Glycyrrhizae, and ratio is 3: 1; Described nitric oxide donors is characterised in that donor is an arginine; The ratio of nitric oxide donors and Chinese herbal and crude drugs preparations is 1: 5.
Adopt preparation of the present invention to have following tangible characteristics and useful effect:
1. has growth promoting function preferably;
2. can significantly improve the breed survival rate of prawn;
3. can significantly improve non-specific immunity.
Specific embodiment:
The preparation of 1 donor and Chinese herbal medicine additive
Chinese herbal medicine additive disposes voluntarily, the Radix Astragali: Radix Glycyrrhizae=3: 1, and even with arginine then by 5: 1 mixed;
2 experiment shrimp Seedlings
Test is taken from the juvenile prawn that culture in Shenzhen farming section center Aquatic product experiment base with shrimp, and average body is long supports after 7 days temporarily for (4.75 ± 0.51) cm, average weight are (0.77 ± 0.24) g, selects the experiment that is used for of specification basically identical.
3 experiment feedstuffs
Test consists of high-quality fish meal, shrimp shell meal, flour, grouts, yeast powder, Duo Kuang, multidimensional, phagostimulant etc. with normal feedstuff, add Chinese herbal and crude drugs preparations on this basis again, addition is respectively 0.5g/kg (A group), 1.0g/kg (B group) and 1.5g/kg (C group).Each nutritional labeling is roughly as follows: crude protein 40%, crude fat 4%, coarse ash 15%, moisture 12%, crude fibre 5%, calcium 4%, phosphorus 1%.
4 experimental conditions
Experiment is to carry out in the round plastic bucket of 300L, the effective water body 250L of experimental session.In order to prevent that testing shrimp jumps out, be stamped the shading net order above the experiment bucket.
5 statistical indicators
Through the breed of 60d, the growth statistical indicator is as follows.
Day growth speed=(last average weight-initial average weight eventually)/test natural law;
Relative rate is rate=(end weight-initial weight)/initial weight * 100% heavily;
The weight of the feed coefficient=feedstuff of throwing something and feeding/shrimp weight increase amount;
Survival rate=results mantissa/put in a suitable place to breed mantissa * 100%.
6 immune indexes are measured
After the off-test, each experimental group is randomly drawed the muscle of 3 tails test prawn, the back homogenate in ice bath of weighing, add physiological saline solution, make concentration reach 0.1g/ml, under 4 ℃ of conditions, behind the centrifugal 10min of 4000r/min, remove post precipitation and be Litopenaeus vannamei muscular tissue extracting solution.Be positioned in-20 ℃ of refrigerators to be measured.Select for use superoxide dismutase (SOD), acid phosphatase (ACP), lysozyme (LZM), antibiotic vigor, phenol oxidase (PO) and nitricoxide synthase (NOS) active as the nonspecific immunity index.
7 artificial challenges experiment
After experiment finishes, randomly draw 30 tails for every group and be used for artificial challenge's experiment, to inquire into the protective rate of Chinese herbal and crude drugs preparations Litopenaeus vannamei.The infection method is as follows: the white spot syndrome virus crude extract with 100 times of PBS buffer dilutions, is expelled in the prawn abdominal muscles, and every endnote is penetrated 100 μ l.Observe 7 days cumulative mortality.
8 experimental results
Growth result sees Table 1, and immune indexes the results are shown in Table 2, and the artificial challenge the results are shown in Table 3.
Table 1 growth experiment result
| Day growth speed | Relative rate is rate (%) heavily | Feed coefficient | Survival rate (%) | |
| Matched group | ?0.01333±0.00076 a | 48.81±5.13 a | 1.93±0.21 a | 86.67±4.99 a |
| A | ?0.01406±0.00105 b | 55.23±4.56 b | 1.78±0.32 b | 89.33±5.52 a |
| B | ?0.01633±0.00119 c | 63.02±3.33 c | 1.69±0.42 b | 94.00±2.70 b |
| C | ?0.01600±0.00098 c | 62.35±4.88 c | 1.71±0.36 b | 95.67±3.47 b |
Annotate: different lowercase alphabet differentials different significantly (P＜0.05) in the same column.Down together.
The influence of ACP, LZM, SOD, phenol oxidase and disinfection vitality in the table 2. pair Litopenaeus vannamei muscle
| Immune indexes | Matched group | The A group | The B group | The C group |
| ACP | 3.97±0.65 b | 4.86±0.64 b | 6.21±0.35 a | 9.95±1.39 c |
| LZM | 7.29±4.92 a | 12.02±1.96 a | 25.13±17.62 b | 34.06±4.55 b |
| SOD | 127.91±9.04 a | 122.20±8.06 ab | 113.70±10.42 b | 134.89±4.65 c |
| NOS | 5.37±1.52 a | 5.78±0.95 a | 7.12±1.44 b | 8.56±0.67 c |
| Phenol oxidase | 1.33±0.09 a | 1.26±0.11 a | 1.65±±0.23 b | 1.74±0.13 b |
| Disinfection vitality | 0.183±0.014 a | 0.234±0.011 b | 0.328±0.014 c | 0.387±0.017 d |
Table 2 artificial challenge result (the dead mantissa of accumulative total)
| Natural law | Matched group | The A group | The B group | The C group |
| 1 | 0 | 0 | 0 | 0 |
| 2 | 0 | 0 | 0 | 0 |
| 3 | 1 | 0 | 0 | 0 |
| 4 | 2 | 1 | 0 | 0 |
| 5 | 4 | 2 | 2 | 1 |
| 6 | 10 | 6 | 4 | 3 |
| 7 | 21 | 12 | 8 | 4 |
As can be seen from Table 1, after the interpolation immunostimulant, no matter be day growth speed, individual rate of body weight gain, feed coefficient, or the survival rate aspect all obviously is better than matched group; As can be seen from Table 2, after the interpolation immunostimulant, each immune indexes all has rising in various degree; As can be seen from Table 3, after the interpolation immunostimulant, the survival rate behind the artificial challenge improves greatly.Confirm that this immunostimulant has the effect of growth promotion, raising survival rate and enhancing premunition.
Claims (4)
1, a kind of nitric oxide donors and Chinese herbal and crude drugs preparations is characterized in that the nonspecific immunity of prawns purposes: it is to mix by a certain percentage with nitric oxide donors and Chinese herbal and crude drugs preparations, can regulate nonspecific immunity of prawns.
2, nitric oxide donors according to claim 1 and Chinese herbal and crude drugs preparations is characterized in that the nonspecific immunity of prawns purposes: the composition of Chinese herbal and crude drugs preparations is the Radix Astragali and Radix Glycyrrhizae, and ratio is 3: 1.
3, nitric oxide donors according to claim 1 and Chinese herbal and crude drugs preparations is characterized in that the nonspecific immunity of prawns purposes: donor is an arginine.
4, nitric oxide donors according to claim 1 and Chinese herbal and crude drugs preparations is characterized in that the nonspecific immunity of prawns purposes: the ratio of nitric oxide donors and Chinese herbal and crude drugs preparations is 1: 5.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200710026468 CN101007049A (en) | 2007-01-23 | 2007-01-23 | Application of nitric oxide donor and Chinese herbal preparation on nonspecific immune function of prawns |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104012779A (en) * | 2014-06-25 | 2014-09-03 | 无锡市朗邦生物科技有限公司 | Preparation method and application of immune preparation capable of enhancing immunities of fishes, prawns and crabs |
| CN104206710A (en) * | 2014-08-15 | 2014-12-17 | 亚太兴牧(北京）科技有限公司 | Traditional Chinese medicinal feed additive containing N-carbamylglutamic acid, and its application |
| CN114868846A (en) * | 2022-04-25 | 2022-08-09 | 闽江学院 | Application of exogenous nitric oxide donor GSNO as feed additive |
-
2007
- 2007-01-23 CN CN 200710026468 patent/CN101007049A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104012779A (en) * | 2014-06-25 | 2014-09-03 | 无锡市朗邦生物科技有限公司 | Preparation method and application of immune preparation capable of enhancing immunities of fishes, prawns and crabs |
| CN104206710A (en) * | 2014-08-15 | 2014-12-17 | 亚太兴牧(北京）科技有限公司 | Traditional Chinese medicinal feed additive containing N-carbamylglutamic acid, and its application |
| CN104206710B (en) * | 2014-08-15 | 2017-05-17 | 亚太兴牧(北京）科技有限公司 | Traditional Chinese medicinal feed additive containing N-carbamylglutamic acid, and its application |
| CN114868846A (en) * | 2022-04-25 | 2022-08-09 | 闽江学院 | Application of exogenous nitric oxide donor GSNO as feed additive |
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Open date: 20070801 |