CN101002575A - Method for producing alfa sensing matter and its application for controlling Huhua Micao grass - Google Patents
Method for producing alfa sensing matter and its application for controlling Huhua Micao grass Download PDFInfo
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- CN101002575A CN101002575A CN200610161387.4A CN200610161387A CN101002575A CN 101002575 A CN101002575 A CN 101002575A CN 200610161387 A CN200610161387 A CN 200610161387A CN 101002575 A CN101002575 A CN 101002575A
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- reed
- allelochemical
- spartina alterniflora
- alterniflora loisel
- extract
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- 238000004519 manufacturing process Methods 0.000 title claims description 6
- 244000025254 Cannabis sativa Species 0.000 title abstract 2
- 235000014676 Phragmites communis Nutrition 0.000 claims abstract description 44
- 238000000034 method Methods 0.000 claims abstract description 12
- 241001149258 Sporobolus alterniflorus Species 0.000 claims description 56
- 239000003627 allelochemical Substances 0.000 claims description 24
- 238000000354 decomposition reaction Methods 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 238000000605 extraction Methods 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 235000012054 meals Nutrition 0.000 claims description 4
- 230000000813 microbial effect Effects 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 229920002678 cellulose Polymers 0.000 claims description 3
- 239000001913 cellulose Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 230000005855 radiation Effects 0.000 claims description 3
- 239000000126 substance Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 description 20
- 241000196324 Embryophyta Species 0.000 description 10
- 244000005700 microbiome Species 0.000 description 9
- 241000894007 species Species 0.000 description 8
- 239000006041 probiotic Substances 0.000 description 7
- 230000000529 probiotic effect Effects 0.000 description 7
- 235000018291 probiotics Nutrition 0.000 description 7
- 230000036531 allelopathy Effects 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 230000035784 germination Effects 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 239000012452 mother liquor Substances 0.000 description 4
- 230000007226 seed germination Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 240000004928 Paspalum scrobiculatum Species 0.000 description 3
- 235000003675 Paspalum scrobiculatum Nutrition 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 241000209094 Oryza Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 241000209504 Poaceae Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000004009 herbicide Substances 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 238000013207 serial dilution Methods 0.000 description 2
- 206010020880 Hypertrophy Diseases 0.000 description 1
- 241000235575 Mortierella Species 0.000 description 1
- 241001558147 Mortierella sp. Species 0.000 description 1
- 241000013557 Plantaginaceae Species 0.000 description 1
- 241000746413 Spartina Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
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- 238000000053 physical method Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- YZHUMGUJCQRKBT-UHFFFAOYSA-M sodium chlorate Chemical compound [Na+].[O-]Cl(=O)=O YZHUMGUJCQRKBT-UHFFFAOYSA-M 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
An allelopathic substance extracted from reed for preventing and eliminating Huhuami grass and its preparing process are disclosed.
Description
One, technical field
This invention belongs to the Ecological Control technical field.
Two, background technology
Spartina alterniflora loisel (Spartina aterniflora) originates in the U.S., belongs to grass family speedwell family, is a kind of perennial sabkha plant that is grown in the intertidal zone.Spartina alterniflora loisel's root system hypertrophy, plant is sturdy tall and big, biomass is big, ground level generally can reach 1.0～2.0m, root system gos deep into can reaching about 1.5m in the soil, have stronger wind resistance disappear wave, protect the effect at beach shore protection, created land with silt, greening seabeach.China's Coastal Areas often was subjected to natural calamities such as storm tide, typhoon at that time and attacked, and the sea wall large tracts of land is impaired, was subjected to very big harm for coastal area economy and people's lives and properties.For this reason, the spartina alterniflora loisel introduces the coastal beach of China at the beginning of 1980, and is protecting beach shore protection, created land with silt, improving many-sides such as beach ecotope and mitigation and made major contribution.On the other hand, the spartina alterniflora loisel is as the alien species with extremely strong fertility, its growth is prosperous, breeding is fast, plant Gao Ermi, strong with the endemic species competition, the view on mud matter light beach and the habitat of native country species have been changed, through the development of more than two decades, the spartina alterniflora loisel forms 50,000 hm in China's Coastal Areas
2Above Alien invasive species advantage group has a series of harmful effects to the bio-diversity and the aquaculture in China coastal beach area, brings a series of negative effect to the coastal area.Therefore, the spartina alterniflora loisel was listed in 16 kinds of invasive species blacklists of China in 2003 by State Bureau of Environmental Protection, and spartina alterniflora loisel's control problem has become global problem.
Control spartina alterniflora loisel's measure at present has:
Physical method: mainly be hand digging and mechanical removal, consume lot of manpower and material resources, not only efficient is low, also needs government's investment to organize manpower and financial resources.
Chemical method: use weed killer herbicide, though effectively simple, there is the serious environmental pollution problem.
Biological control: biological control is meant and utilizes natural enemy or pathogenic microorganisms to prevent and treat Alien invasive species.As Alien invasive species, its natural enemy and pathogenic microorganisms also are denizens, and therefore, there is great ecological risk in biological control, may bring new invasion problem, use not extensive.
Three, summary of the invention
Administer spartina alterniflora loisel's method at present, have many drawbacks.Physical control is wasted time and energy; There is pollution problem in chemical method; Not only there is bigger problems of ecological security in BIOLOGICAL CONTROL, and control efficiency is relatively poor.The problem that the present invention need solve is: utilize the reed allelopathy, from the reed The litter, extract allelochemical, can effectively suppress the growth of peculiar probiotic microorganisms population in the spartina alterniflora loisel group, and can reduce spartina alterniflora loisel's percentage of seedgermination, spartina alterniflora loisel's growth of seedlings there is significant inhibitory effect.By these effects, the reed allelochemical can effectively suppress spartina alterniflora loisel's breeding, thus control spartina alterniflora loisel's expansion.
This laboratory finds that under study for action a kind of distinctive advantage fungi (Mortierella sp.) population is arranged in spartina alterniflora loisel group.This fungi only appears in the spartina alterniflora loisel group, does not find to belong to peculiar population by this fungi in the plant community around the spartina alterniflora loisel.Find that through identifying this bacterial strain is a kind of of Mortierella fungi.Test shows that this bacterium can be improved the survival rate of spartina alterniflora loisel seedling significantly, and as seen comparison has played very big effect for the breeding expansion of rice grass according to having improved 20.27%; After adding this bacterium,, all increase significantly, show that this bacterium plays significant facilitation to spartina alterniflora loisel's growth than control group to growth indexes biomass, plant height, the thick mensuration relatively of stem; Chlorophyll and soluble sugar analysis have been shown that also adding this bacterium obviously is better than control group to the growth of rice grass; The mensuration of POD and PAL has also obtained identical result, shows that this bacterium all plays positive role to spartina alterniflora loisel's resistance and growth.This shows, this fungi is a distinctive microbiologic population in the exotic species Spartina alterniflora group, is spartina alterniflora loisel's rhizosphere symbiotic microorganism, belongs to spartina alterniflora loisel's probio, to growing of spartina alterniflora loisel, and very large positive role is all played in spartina alterniflora loisel's expansion.So we are by the method for probiotic microorganisms in the inhibition spartina alterniflora loisel, thus inhibition spartina alterniflora loisel's growth, control spartina alterniflora loisel's expansion.
Reed is the grass family herbaceos perennial, and cosmopolitan can adapt to different ecotopes, belongs to local species in China, is the representative plant species that constitutes wetland plant group.Reed and spartina alterniflora loisel's ecological niche is overlapped, and forms competition in certain zone and spartina alterniflora loisel.Utilize the allelochemical of reed, can effectively control the spartina alterniflora loisel, both laborsaving, control efficiency is remarkable.
Concrete technical scheme of the present invention is as follows:
1, the production method of reed allelochemical
(1) collection of reed The litter and preliminary working: collect ripe reed The litter in December, with the distilled water rinsing, dry.With micromill it is pulverized, it is standby to cross 20 mesh sieves;
(2) extraction of reed allelochemical: utilize solution extraction and anaerobism decomposition method to extract the allelochemical of reed, the solution extraction is that the distilled water of 10 times of quality of standby hay meal adding is put into closed container, normal temperature 48 hours, and filtration obtains extract, anaerobism decomposition method is that hay meal radiation sterilization 3h under uviol lamp is placed on through in the container of sterilization treatment, the microbial decomposition cellulose medium and the rhizosphere soil that add 10 times of quality, mixing, in 25 ℃ of following constant temperature culture 30 days, obtain extract.
2, the application of reed allelochemical
The reed allelochemical suppresses peculiar probiotic microorganisms population in spartina alterniflora loisel's seed, seedling and the rice grass.The reed allelochemical of 1: 10 concentration of solution extraction obviously reduces percentage of seedgermination, and has suppressed growth of seedlings, has suppressed the growth of spartina alterniflora loisel probio simultaneously significantly.Reed decomposition product most pronounced effects when mother liquid concentration that anaerobism decomposition method is extracted.
(3) the present invention compared with the prior art, its significance progress is:
Plant allelopathy (Allelopathy) also claims allelopathy, the effect of counteracting each other and allelopathy effect, is that plant passes through synthetic and discharges chemical substance and to sprouting of of the same race or xenogenesis plant (comprising microorganism) and the direct or indirect useful or deleterious effects of growing and being produced to environment.Biochemical allelopathy has important effect in the formation of sociales, the succession of community, revegetation.At present, still do not utilize the correlative study report of reed allelochemical control exotic species Spartina alterniflora both at home and abroad.
The present invention utilizes the allelochemical of reed, can reduce spartina alterniflora loisel's seed germination, suppress spartina alterniflora loisel's growth of seedlings, simultaneously distinctive probiotic microorganisms population in the spartina alterniflora loisel group is produced significant inhibitory effect, disturbed the microbial environment of spartina alterniflora loisel's root system, the spartina alterniflora loisel has been grown be suppressed.
By the reed allelochemical, can effectively control spartina alterniflora loisel's growth, suppress spartina alterniflora loisel's transition expansion, and can make reed community substitute the spartina alterniflora loisel, the sustainable development of coastal area there is positive role.The present invention has overcome physics in the past, chemistry and biological control spartina alterniflora loisel's deficiency, has both avoided pollution and ecological risk, has realized the Ecological Control to the spartina alterniflora loisel again.
Four, embodiment
Collect the The litter of ripe reed in winter, utilize solution extraction and anaerobism decomposition method to extract the allelochemical of reed, and the peculiar probiotic microorganisms population of spartina alterniflora loisel's seed, seedling and spartina alterniflora loisel is acted on by allelochemical, thereby realize the control to the spartina alterniflora loisel, embodiment is as follows:
1, the collection of reed The litter and preliminary working: collect ripe reed The litter in December, with the distilled water rinsing, dry.With micromill it is pulverized then, it is standby to cross 20 mesh sieves;
2, reed water extract preparation: the dry powder that 1 step is obtained places closed container and adds the distilled water of 5 times of quality, adds to be placed under the normal temperature and leaves standstill 48h, and filtering at last and obtaining mass concentration is 1: 5 mother liquor;
3, reed decomposition product: dry powder radiation sterilization 3h under uviol lamp that 1 step is obtained is placed on through in the container of sterilization treatment, add 10 times of quality microbial decomposition cellulose medium and rhizosphere soil, mixing in 25 ℃ of following constant temperature culture 30 days, obtains mother liquor;
4, the reed allelochemical is to the effect of spartina alterniflora loisel's seed: with the mother liquor serial dilution is 50% and 25%, obtains three concentration gradients altogether.Seed inserted carry out seed germination test in three kinds of mother liquors and the control group distilled water.The germination rate formula is: germination rate=germinative number/sum * 100%.The result shows that reed water extract concentration is that 1: 10 o'clock inhibition effect reaches best, and germination rate drops to 23% by 36% of control group, and effect is remarkable.Germination rate dropped to 28% by 36% of control group when reed decomposition production concentration was mother liquid concentration, and effect is remarkable;
5, the reed allelochemical is to the effect of spartina alterniflora loisel seedling: with the mother liquor serial dilution is 50% and 25%, obtains three concentration gradients altogether.Seedling is inserted three kinds of mother liquors and control group heats up in a steamer and carries out the growth of seedling test in the water.20 ℃ of following constant temperature culture 96 hours are calculated the influence that extract is long to seedling and root is grown.The result shows, the processing of three concentration of reed water extract is to the not obviously effect of seedling acrial part, but extract concentrations is but to suppress the growth of seedling underground part root division significantly 1: 10 time, by the 6.40cm of the control group 4.93cm under this concentration.Reed decomposition product is under mother liquid concentration, and is not only remarkable to the influence of seedling acrial part, and the growth of under ground portion root is had utmost point remarkable influence.By the 6.40cm of the contrast 1.03cm under this concentration, effect is extremely remarkable especially for the 1.44cm of the 1.89cm that acrial part has contrast respectively under this concentration, under ground portion;
6, the reed allelochemical is to the effect of peculiar probiotic microorganisms population in the spartina alterniflora loisel group: with Martin's medium and extract through after the sterilization treatment, get three kinds of treatment fluids of 13.5ml medium and 1.5ml and in culture dish of the same size, mix the formation flat board, the careless probio of inoculation rice is measured the bacterial plaque diameter after three days.The result shows that three concentration effects of reed water extract are all obvious, are in concentration at 1: 10 o'clock, and effect is best, is reduced to 2.90cm by the 3.75cm that contrasts.Three concentration of reed decomposition product are similar to the water extract effect, and obvious inhibitory action is all arranged, and effect is best under 25% concentration, and colony diameter is reduced to 3.20cm by 3.75cm.
Can obtain by above, the allelochemical that the reed The litter is extracted can reduce spartina alterniflora loisel's germination rate, suppress the careless growth of seedlings of rice, also significantly suppress simultaneously the growth of peculiar probiotic microorganisms group in the spartina alterniflora loisel group, energy good restraining spartina alterniflora loisel's breeding and expansion, thus the spartina alterniflora loisel effectively controlled.
Claims (2)
1, a kind of production method of reed allelochemical, method is made up of following step:
(1) collection of reed The litter and preliminary working: collect ripe reed The litter in December, with the distilled water rinsing, dry, with micromill it is pulverized, mistake 20 mesh sieves are standby;
(2) extraction of reed allelochemical: utilize solution extraction and anaerobism decomposition method to extract the allelochemical of reed, the solution extraction is that the distilled water of 10 times of quality of standby hay meal adding is put into closed container, normal temperature 48 hours, and filtration obtains extract, anaerobism decomposition method is that hay meal radiation sterilization 3h under uviol lamp is placed on through in the container of sterilization treatment, the microbial decomposition cellulose medium and the rhizosphere soil that add 10 times of quality, mixing, in 25 ℃ of following constant temperature culture 30 days, obtain extract.
2, the application of reed allelochemical in the control exotic species Spartina alterniflora of producing according to the production method of the described a kind of reed allelochemical of claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101613874A CN100426970C (en) | 2006-12-26 | 2006-12-26 | Method for producing alfa sensing matter and its application for controlling Huhua Micao grass |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006101613874A CN100426970C (en) | 2006-12-26 | 2006-12-26 | Method for producing alfa sensing matter and its application for controlling Huhua Micao grass |
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| CN101002575A true CN101002575A (en) | 2007-07-25 |
| CN100426970C CN100426970C (en) | 2008-10-22 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101982047A (en) * | 2010-09-09 | 2011-03-02 | 孙兆远 | Method for cultivating wheat seedlings by exogenous phenolic acid intimidation method |
| CN103004428A (en) * | 2012-12-17 | 2013-04-03 | 华东师范大学 | Method for removing invasive alien plants from reed communities |
| CN103197052A (en) * | 2013-04-23 | 2013-07-10 | 南京林业大学 | Method for verifying fern gametophyte allelopathy through membrane permeability method |
| CN105028412A (en) * | 2015-07-22 | 2015-11-11 | 兰州大学 | Plant allelopathy inhibitor for controlling Gansu pedicularis |
| CN105123669A (en) * | 2015-09-10 | 2015-12-09 | 上海大学 | Ecological management method for utilizing secondary metabolites of plants to inhabit spartina alterniflora invasion |
| CN105784613A (en) * | 2016-03-07 | 2016-07-20 | 江苏大学 | Method for testing water environmental response characteristic of allelopathic effect of reeds on phalaris arundinacea |
| CN109247350A (en) * | 2017-07-12 | 2019-01-22 | 上海利盛生化有限公司 | A kind of herbicide of environment-friendly high-efficiency and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003212703A (en) * | 2002-01-11 | 2003-07-30 | Kiso Kogyo Kk | Grass growth-suppressing board |
| CN1282612C (en) * | 2003-11-07 | 2006-11-01 | 清华大学 | Application of bulrush as algae inhibiting material |
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2006
- 2006-12-26 CN CNB2006101613874A patent/CN100426970C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101982047A (en) * | 2010-09-09 | 2011-03-02 | 孙兆远 | Method for cultivating wheat seedlings by exogenous phenolic acid intimidation method |
| CN101982047B (en) * | 2010-09-09 | 2013-02-13 | 孙兆远 | Method for cultivating wheat seedlings by exogenous phenolic acid intimidation method |
| CN103004428A (en) * | 2012-12-17 | 2013-04-03 | 华东师范大学 | Method for removing invasive alien plants from reed communities |
| CN103197052A (en) * | 2013-04-23 | 2013-07-10 | 南京林业大学 | Method for verifying fern gametophyte allelopathy through membrane permeability method |
| CN105028412A (en) * | 2015-07-22 | 2015-11-11 | 兰州大学 | Plant allelopathy inhibitor for controlling Gansu pedicularis |
| CN105123669A (en) * | 2015-09-10 | 2015-12-09 | 上海大学 | Ecological management method for utilizing secondary metabolites of plants to inhabit spartina alterniflora invasion |
| CN105784613A (en) * | 2016-03-07 | 2016-07-20 | 江苏大学 | Method for testing water environmental response characteristic of allelopathic effect of reeds on phalaris arundinacea |
| CN105784613B (en) * | 2016-03-07 | 2018-10-09 | 江苏大学 | A method of test reed is to Phalaris grass allelopathy effect water environmental response characteristics |
| CN109247350A (en) * | 2017-07-12 | 2019-01-22 | 上海利盛生化有限公司 | A kind of herbicide of environment-friendly high-efficiency and preparation method thereof |
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| CN100426970C (en) | 2008-10-22 |
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