CN1009867B - Colour polarization and interference contrast lighting device of microscope - Google Patents
Colour polarization and interference contrast lighting device of microscopeInfo
- Publication number
- CN1009867B CN1009867B CN 87106138 CN87106138A CN1009867B CN 1009867 B CN1009867 B CN 1009867B CN 87106138 CN87106138 CN 87106138 CN 87106138 A CN87106138 A CN 87106138A CN 1009867 B CN1009867 B CN 1009867B
- Authority
- CN
- China
- Prior art keywords
- microscope
- support
- light source
- ring
- polarizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
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- Microscoopes, Condenser (AREA)
Abstract
The present invention discloses a chromatic polarization interference difference illumination device for a microscope, which is designed according to the principle of multiple-beam interference and the principle of polarized light interference. The chromatic polarization interference difference illumination device for a microscope and an analyzer are together added on an ordinary optical microscope. Therefore, the microscope with single function is made into a multifunctional microscope which not only has the function of the simple polarization microscope but also has the effect of the differential interference contrast microscope. The present invention has the advantages of simple device, low cost and convenient operation. The chromatic polarization interference difference illumination device for a microscope can be widely applied to the sciences such as the biology, the medicine, the chemistry, the pharmacy, the histology, the cytology, etc. The scientific research institution, the basic unit, and the academy all have the ability to purchase the chromatic polarization interference difference illumination device for a microscope.
Description
The present invention is a kind of microscope attachment device, is installed on the ordinary optical microscope, thereby makes unifunctional ordinary optical microscope become the function that both has simple polarizing microscope, has the multi-functional microscope of the effect of differential interference contrast microscope again concurrently.
Polarizing microscope and differential interference contrast microscope are two kinds of microscopes with special optical performance, are to produce according to polarized light and two-beam interference principle, and the principal feature of its structure is to be provided with the polarizer in the mirror, analyzer and promise MAERSK (Nomarski) prism.The not only manufacturing cost height of promise MAERSK prism own, and many and universal microscope systems selling, thereby its parts price is also high make the unable purchase of general cause and R﹠D institution like this, thereby have limited applying of they.
The objective of the invention is according to multiple-beam interference principle and polarized light interference principle, increase by one is simple in structure on ordinary optical microscope, cheap attachment device, thus make common optical microscope both have the function of simple and easy polarizing microscope, have the effect of differential interference contrast microscope again concurrently.
The objective of the invention is that following mode realizes, go up the attachment device (see figure 1) that installation is made up of colour polarization and interference contrast lighting device (2) and analyzer (3) at ordinary optical microscope (1), colour polarization and interference contrast lighting device is mainly by the condensation light source (4) that is contained on the support (8), be positioned at the polarizer (5) of condensation light source (4) top, colour cast vibration plate (6) is formed (see figure 2) with the optically focused vertex mirror (7) that is contained on the top Jing Gai (9).
That condensation light source (4) adopts is 2.2V, the bulb of 0.54A, and it can reach the rotation of itself by spiral (11) in fixed, thereby makes light source centering.Regulating ring (12) is arranged outside the polarizer (5), rotate regulating ring (12) and can drive 120 ° of rotations of polarizer do, be convenient to detect disdiaclast.Colour cast vibration plate (6) is for drawing the crystal slab that forms with transparent plastic film, and its effect is to make the light that penetrates from light source produce chromatic polarization.The outer coloured polarization plates regulating ring (13) of colour cast vibration plate (6) rotates regulating ring (13) and can drive 120 ° of rotations of colour cast vibration plate do, and the field of microscope background colour is changed continuously.
Fig. 1, this device is scheme of installation on ordinary optical microscope.
Fig. 2, the colour polarization and interference contrast lighting device structural representation.
The red blood cell photo that Fig. 3, ordinary optical microscope take.
Fig. 4, ordinary optical microscope add the red blood cell photo that takes behind this device.
Fig. 5, ordinary optical microscope add the red blood cell photo that has " pockmark " that this device back is taken.
With reference to the accompanying drawings content of the present invention is described in detail again.
With reference to Fig. 1, analyzer (3) can be placed between microscopical object lens and the eyepiece, also can be placed on the eyepiece position.Reflective mirror on the microscope is taken off, again condenser (its external diameter is unified to be 37mm) is taken off from mirror holder, then colour polarization and interference contrast lighting device (2) is installed on the condenser mirror holder.
With reference to Fig. 2, polarizer regulating ring (12), colour cast vibration plate regulating ring (13) is enclosed within outside the support (8), the polarizer (5), colour cast vibration plate (6) is embedded in respectively in the bracket of the interior garden shape filler ring (15) of support (8), (16), garden shape filler ring (15) is placed on the interior bank of support (8), and garden shape filler ring (16) is placed on the garden shape filler ring (15).Polarizer regulating ring (12) is passed in screw (17), (18), and chromatic polarization regulating ring (13), its head withstand filler ring (15), (16) respectively.Rotate regulating ring (12), (13) then can drive the polarizer (5) and colour cast vibration plate (6) carries out 120 ° of rotations in the horizontal direction.Between top Jing Gai (9) and connecting cylinder (14) with being threaded, between support (8) and connecting cylinder (14) also with being threaded.The long 32mm of connecting cylinder (14), internal diameter 25.4mm, external diameter 36mm.Top Jing Gai, regulating ring, connecting cylinder, support, filler ring is all made with alloy aluminum.
With reference to Fig. 3 Fig. 4, observe red blood cell with ordinary optical microscope, red blood cell does not have stereoscopic sensation, and observe after having adopted this attachment device, normocyte is the garden plate-like of embossment sample two sides depression, and the surface is very smooth, and this phenomenon has only with differential interference contrast microscope and could observe out.
Excise the patient of spleen, have the erythrocyte surface of significant proportion to show " cavity ", and the normal person has had the red blood cell of this kind cavity rare.This kind has the red blood cell of " cavity " to observe not come out under ordinary optical microscope, but under differential interference contrast microscope, more than 1～10 garden shape shrinkage pools that differ in size appear in the surface of this cell, form is like pockmark, this kind " pockmark " is actually " cavity " on the red blood cell, and this is the phenomenon that the optical characteristics of differential interference contrast microscope causes.As can be seen from Fig. 5, adopted the ordinary optical microscope of this attachment device to observe the red blood cell that has " pockmark ", " pockmark " on it is high-visible, from also finding out, adds that the simple microscope of this device has the effect of differential interference contrast microscope here.
Ordinary optical microscope adds the optical filter that does not need various wavelength behind this attachment device, field of microscope just can present the required background color of the best contrast of various samples, if there is disdiaclast to exist, distinct different color then appears, and can make the certain stereoscopic sensation of pattern generating, the result of similar low power scanning electron microscopic observation, it is highly sensitive that it detects disdiaclast.
This apparatus structure is simple, produces easily, and is cheap.This attachment device of ordinary optical microscope adapted makes simple microscope both have simple polarizing microscope function, has the effect of differential interference contrast microscope again concurrently.Can in ambits such as biology, medical science, chemistry, materia medica, histology, cytology, use.Because it is cheap, general cause, R﹠D institution, primary care health unit, even the purchase of all having the ability of general secondary school have social benefit and remarkable economic efficiency widely.
Claims (7)
1, a kind of colour polarization and interference contrast lighting device that is installed on the ordinary optical microscope 1, it includes analyzer 3, condensation light source 4, the polarizer 5, it is characterized in that adjustable condensation light source 4 is contained on the support 8, the polarizer 5 is positioned at condensation light source 4 tops, and colour cast vibration plate 6 is positioned at the polarizer 5 tops, and optically focused vertex mirror 7 is embedded in the top mirror lid 9, adopt spiral way to connect with connecting cylinder 14 between support 8, the top mirror lid 9, condensation light source 4 is a bulb.
2, device according to claim 1, it is characterized in that the polarizer 5 is embedded in the bracket of circular filler ring 15, the polarizer regulating ring 12 that is enclosed within outside the support 8 is arranged outside it, screw 17 passes regulating ring 12, its head withstands filler ring 15, and filler ring 15 is placed on the bank in the support 8.
3, device according to claim 1, it is characterized in that colour cast vibration plate 6 is embedded in the bracket of circular filler ring 16, the colour cast vibration plate regulating ring 13 that is enclosed within outside the support 8 is arranged outside it, screw 18 passes regulating ring 13, its head withstands filler ring 16, and filler ring 16 is placed on the filler ring 15.
4,, it is characterized in that colour cast vibration plate 6 is for drawing the crystal slab that forms with transparent plastic film according to claim 1,3 described devices.
5, device according to claim 1, that it is characterized in that condensation light source 4 employings is 2.2V, the bulb of 0.54A, small electric bulb itself can rotate, and the fixed middle spiral 11 of regulating light source centering is installed on the support 8.
6, device according to claim 1 is characterized in that connecting cylinder 14 long 32mm, internal diameter 25.4mm, external diameter 36mm.
7, according to claim 2.3 or 6 described devices, it is characterized in that support 8 top mirror lids 9, connecting cylinder 14, circular filler ring 15,16 are all used the alloy aluminum material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87106138 CN1009867B (en) | 1987-09-04 | 1987-09-04 | Colour polarization and interference contrast lighting device of microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87106138 CN1009867B (en) | 1987-09-04 | 1987-09-04 | Colour polarization and interference contrast lighting device of microscope |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN87106138A CN87106138A (en) | 1988-10-26 |
| CN1009867B true CN1009867B (en) | 1990-10-03 |
Family
ID=4815597
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 87106138 Expired CN1009867B (en) | 1987-09-04 | 1987-09-04 | Colour polarization and interference contrast lighting device of microscope |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1009867B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101069115B (en) * | 2004-04-16 | 2010-05-12 | 奥本大学 | Microscope illumination device and adapter therefor |
| WO2007064959A2 (en) | 2005-12-01 | 2007-06-07 | Auburn University | High resolution optical microscope |
-
1987
- 1987-09-04 CN CN 87106138 patent/CN1009867B/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| CN87106138A (en) | 1988-10-26 |
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| CF01 | Termination of patent right due to non-payment of annual fee |