CN1229927A - Laser confocal scanning microscope - Google Patents
Laser confocal scanning microscope Download PDFInfo
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- CN1229927A CN1229927A CN 98111188 CN98111188A CN1229927A CN 1229927 A CN1229927 A CN 1229927A CN 98111188 CN98111188 CN 98111188 CN 98111188 A CN98111188 A CN 98111188A CN 1229927 A CN1229927 A CN 1229927A
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Abstract
A confocal laser scan microscope suitable for pathologic observation and patho-anatomy, biotics, genetics and blood research features that a semi-reflecting and semi-transmitting lens is used to integrat laser lighting path for specimen, the path of light signals scattered from specimen and observating light path in a whole for simpler light path, compacter structure, and smaller size.
Description
The present invention relates to a kind of microscope, particularly a kind of confocal laser scanning microscope.
Confocal laser scanning microscope is the new and high technology microscope of up-to-date appearance, and its feature is that confocal principle and high-rate laser scanning technique are combined, and obtaining the layering image of object, the three-dimensional appearance of sample is finally exported in machine image processing as calculated again.Wherein optical system is very complicated, and it need be integrated sample laser lighting light path, sample scattering light signal light path and observation optical path three.At present, all adopt the not only complicated but also unmanageable double color reflection mirror of multi-disc to realize the purpose of three light path unifications in the world, as the Leica type of Germany's product and the F900e confocal laser scanning microscope of U.S.'s product.The Leica type confocal laser scanning microscope that Germany produces, be characterized in: 1, horizontal and vertical scanning is finished with a scanner; 2, the laser of incident is introduced with the double color reflection mirror reflection; 3, scattered light signal is drawn with the transmission of double color reflection mirror; 4, use a plurality of detectors; 5, complex structure, bulky.The F900e type confocal laser scanning microscope that the U.S. produces, similar to the Leica type of Germany basically, be characterized in: 1, scanner head is movable, can take off or load onto; 2, with two double color reflection mirrors; 3, use two detectors.
The purpose of this invention is to provide and a kind of three light paths are integrated as far as possible, thereby make the small and exquisite confocal laser scanning microscope of compact conformation, volume of total system.
The object of the present invention is achieved like this: this confocal laser scanning microscope, it is characterized in that sending into condenser lens through conduction optical fiber from the laser that laser instrument sends, after condenser lens, on optical axis, be disposed with optical filter, diffusing panel, aperture light orchid, catoptron, collimation lens, semi-reflection and semi lens, XY reflective scan device, microcobjective, bearing table and depth shifter, illuminating lens and lighting source; On the transmission optical axis of semi-reflection and semi lens, be disposed with optical filter, catoptron and microcobjective, after microcobjective, scattered light signal sent into photomultiplier, through amplification processor and computing machine signal is handled then by detection optical fiber; Between catoptron and collimation lens, be provided with reflecting prism, on the optical axis of reflecting prism, be provided with observing system.Aperture light orchid is positioned on the object plane of the focus point of collimation lens and condenser lens; The end face of detection optical fiber input end is positioned on the focus point of microcobjective; Diffusing panel is tried one's best in sample laser lighting light path near on the convergent point of light beam.
The present invention has only just realized the purpose that sample laser lighting light path, sample scattering light signal light path and observation optical path are integrated with the semi-reflection and semi lens of 45 ° of settings.Laser line focus lens pass optical filter and diffusing panel post-concentration to aperture light orchid, after mirror reflects again through the reflection of semi-reflection and semi lens, invest X and Y reflective scan device, focus on through microcobjective then and be detected on the object, this is sample laser lighting light path.Light signal by the object scattering returns along same light path, sees through the semi-reflection and semi lens, and the wavelength of scattered light signal is selected through filter plate, converges to detection optical fiber and reach photomultiplier through microcobjective again to receive, and this is sample scattering light signal light path.When being detected object by incandescent lighting, light returns along same light path, through semi-reflection and semi reflection from lens, after the prismatic reflection that is reflected reach observing system, this is observation optical path.
The present invention compared with prior art, its remarkable advantage is: light path is simplified, compact conformation, volume is less, and is with low cost.The present invention can be used for pathological observation various hospitals, medical college and medical science, material science and the research of agricultural science mechanism etc. relevant with dissection, life science, genetics research, blood research etc.
The invention will be further described below in conjunction with drawings and Examples:
Accompanying drawing is light path of the present invention and structural representation.
Embodiment: peak power is the Argon ion laser 1 of 50mW, and sending beam of laser is that the conduction optical fiber of 200 μ m is sent into condenser lens 2 through core diameter.Line focus lens 2 also focus on blue 5 places of aperture light that the aperture is 0.5mm through the diffusing panel 4 that optical filter 3 passes rotation, catoptron 6 through 45 ° of settings reflects again, by collimation lens 7 with laser beam datum, then through the reflection of the semi-reflection and semi lens 8 of 45 ° of settings, again through the reflective scan of XY reflective scan device 9, at last by microcobjective 10 laser focusing to being detected on the sample.
The sample scattering light signal returns along same light path, see through the semi-reflection and semi lens after optical filter 14 filters again through catoptron 15 reflections and microcobjective 16 focusing, send into the detection optical fiber that core diameter has only several microns, by detection optical fiber scattered light signal is delivered to photomultiplier 17 and receive, behind amplification processor 18, be defeated by computing machine 19 and handle.When the observer detected by an unaided eye, the light that lighting source 13 sends removed the sample that throws light on through illuminating lens 12 optically focused, through the reflection of semi-reflection and semi lens 8, entered observing system 21 through reflecting prism 20 again and observed like a cork for the observer.
Under the instruction of computing machine 19 was handled, XY reflective scan device 9 was with the velocity sweeping of 300Hz~1200Hz, to obtain the image of a certain tomography of sample.Equally, under the instruction of computing machine 19 was handled, depth shifter 11 was adjusted the depth height of sample automatically, thereby obtained the image of the different tomographies of sample.Last computing machine 19 deals with these faultage images, to obtain the three-dimensional appearance of sample.
Claims (4)
1, a kind of confocal laser scanning microscope, it is characterized in that sending into condenser lens [2] through conduction optical fiber, be disposed with optical filter [3], diffusing panel [4], aperture light orchid [5], catoptron [6], collimation lens [7], semi-reflection and semi lens [8], XY reflective scan device [9], microcobjective [10], bearing table and depth shifter [11], illuminating lens [12] and lighting source [13] along optical axis afterwards at condenser lens [2] from the laser that laser instrument [1] sends; Transmission optical axis along semi-reflection and semi lens [8] is disposed with optical filter [14], catoptron [15] and microcobjective [16], at microcobjective [16] afterwards, by detection optical fiber scattered light signal is sent into photomultiplier [17], through amplification processor [18] and computing machine [19] signal is handled then; Between catoptron [6] and collimation lens [7], be provided with reflecting prism [20], be provided with observing system [21] along the optical axis of reflecting prism [20].
2, confocal laser scanning microscope according to claim 1 is characterized in that aperture light orchid [5] is positioned on the object plane of the focus point of collimation lens [7] and condenser lens [2].
3,, it is characterized in that the end face of detection optical fiber input end is positioned on the focus point of microcobjective [16] according to claim 1,2 described confocal laser scanning microscopes.
4,, it is characterized in that diffusing panel [4] is positioned at the beam convergence point of sample laser lighting light path according to claim 1,2 described confocal laser scanning microscopes.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN 98111188 CN1110717C (en) | 1998-03-23 | 1998-03-23 | Laser confocal scanning microscope |
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CN 98111188 CN1110717C (en) | 1998-03-23 | 1998-03-23 | Laser confocal scanning microscope |
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CN1229927A true CN1229927A (en) | 1999-09-29 |
CN1110717C CN1110717C (en) | 2003-06-04 |
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CN 98111188 Expired - Fee Related CN1110717C (en) | 1998-03-23 | 1998-03-23 | Laser confocal scanning microscope |
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CN101387008B (en) * | 2007-09-14 | 2011-05-04 | 清华大学 | Carbon nanotube growing apparatus |
CN102385153A (en) * | 2010-08-27 | 2012-03-21 | 索尼公司 | Microscope and filter inserting method |
CN102494707A (en) * | 2011-10-31 | 2012-06-13 | 中国科学院长春光学精密机械与物理研究所 | Illuminating system for absolute grating scale |
CN102566025A (en) * | 2010-12-09 | 2012-07-11 | 苏州生物医学工程技术研究所 | Microscope capable of maintaining consistency of viewing field illuminance |
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CN1793874B (en) * | 2005-12-14 | 2010-05-05 | 中国科学院上海技术物理研究所 | Equipment and method for measuring photoelectric performance of semiconductor nanometer structure |
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1998
- 1998-03-23 CN CN 98111188 patent/CN1110717C/en not_active Expired - Fee Related
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US8077318B2 (en) | 2007-09-14 | 2011-12-13 | Tsinghua University | Apparatus for manufacturing carbon nanotubes |
CN101387008B (en) * | 2007-09-14 | 2011-05-04 | 清华大学 | Carbon nanotube growing apparatus |
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CN102566025A (en) * | 2010-12-09 | 2012-07-11 | 苏州生物医学工程技术研究所 | Microscope capable of maintaining consistency of viewing field illuminance |
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CN102494707A (en) * | 2011-10-31 | 2012-06-13 | 中国科学院长春光学精密机械与物理研究所 | Illuminating system for absolute grating scale |
CN103674926A (en) * | 2012-08-29 | 2014-03-26 | 日立视听媒体股份有限公司 | Optical device |
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CN106353535A (en) * | 2016-10-10 | 2017-01-25 | 中国科学院深圳先进技术研究院 | In-situ photoelectric multifunctional coupling atomic force microscope testing system |
CN110023811A (en) * | 2016-10-17 | 2019-07-16 | 卡尔蔡司显微镜有限责任公司 | For for probe microscope light optical module, for the method and microscope of microexamination |
US11221472B2 (en) | 2016-10-17 | 2022-01-11 | Carl Zeiss Microscopy Gmbh | Optical group for detection light for a microscope, method for microscopy, and microscope |
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