CN1121624C - Color lining microscope - Google Patents

Color lining microscope Download PDF

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Publication number
CN1121624C
CN1121624C CN01108514A CN01108514A CN1121624C CN 1121624 C CN1121624 C CN 1121624C CN 01108514 A CN01108514 A CN 01108514A CN 01108514 A CN01108514 A CN 01108514A CN 1121624 C CN1121624 C CN 1121624C
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China
Prior art keywords
condenser
microscope
focal plane
lens
light path
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Expired - Fee Related
Application number
CN01108514A
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Chinese (zh)
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CN1321904A (en
Inventor
王�华
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Chongqing Normal College
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王�华
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Priority to CN01108514A priority Critical patent/CN1121624C/en
Publication of CN1321904A publication Critical patent/CN1321904A/en
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Publication of CN1121624C publication Critical patent/CN1121624C/en
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Abstract

The present invention relates to a novel phase contrast microscope which comprises an illuminated light path and an imaging light path, wherein the illuminated light path is composed of an electric light source 1, an illuminator 2 and a condensing lens 4; the imaging light path is composed of an objective lens 6 and an eye lens 8. The present invention is characterized in that in the illuminated light path, a coding plate 3 is arranged on the front focal plane of the condensing lens 4; the rear focal plane of the condensing lens 4 is coincided with the object plane of the microscope so that a specimen 5 is positioned on the rear focus of the condensing lens 4. The present invention has the advantages that the problem that color contrast microscopic images are difficult to be coded by a high-power objective lens is solved; under general conditions, objective lenses with different powers can share the same coding plate so as to simplify the structure of the microscope and facilitate operation without using an analyser plate in front of the eye lens.

Description

Novel color lining microscope
Technical field
The present invention relates to optical instrument, particularly a kind of adopted pseudo-color coding technique and spatial filtering technology, have a novel color lining microscope that colour, solid close high contrast micro-image.
Background technology
Color lining microscope has the micro-image feature of colour, embossment sense and high contrast, and its dispense with dyeing just can make micro-sample have colored micro-image.But this microscopical high power objective colorize is difficulty, and its image effect is also many than low power objective weak effect; Simultaneously, different object lens can not use a block encoding plate jointly, and this increases manufacturing cost, use also inconvenient.For example, Chinese patent 92108060.7 disclosed color lining microscopes, though can make the object lens image colorization below 25 times, but make high power objective image color difficulty, image halation is serious, its figure image contrast is poor than the figure image contrast that interference microscopy obtains, and the structure of encoding board is comparatively complicated, and manufacture difficulty is bigger.In order to make microscope obtain the better pictures effect, to the object lens of different numerical apertures, the encoding board of adapted different size size, this mode has increased microscopical cost.
Summary of the invention
The purpose of this invention is to provide that a kind of back focal plane that makes the condenser of color lining microscope overlaps with microscopical object plane, microscopical image effect is greatly improved, and can make the microscopical structure simple more novel color lining microscope that becomes.
Above-mentioned purpose of the present invention is to realize by such technical scheme, it is a kind of novel color lining microscope, comprise illumination path that electric light source, collecting lens, condenser constitute and the imaging optical path part that constitutes by object lens, eyepiece, it is characterized in that: in illumination path, the front focal plane of condenser is provided with encoding board, the back focal plane of condenser overlaps with microscopical object plane, and sample is on the back focus of condenser.
Description of drawings
Said structure of the present invention can further specify by the embodiment that accompanying drawing constitutes.
The present invention has following accompanying drawing:
Accompanying drawing 1 is the optical schematic diagram of the structure of the embodiment of the invention 1;
Accompanying drawing 2 is the optical schematic diagram of the structure of the embodiment of the invention 2;
Accompanying drawing 3 is the structural representation of encoding board 3 of the present invention.
Embodiment is referring to accompanying drawing: the novel color lining microscope among the figure, comprise illumination path that electric light source 1, collecting lens 2, condenser 4 constitute and the imaging optical path that constitutes by object lens 6, eyepiece 8, it is characterized in that: in illumination path, encoding board 3 is set on the front focal plane of condenser 4, the back focal plane of condenser overlaps with microscopical object plane, and sample 5 is on the back focus of condenser.
The embodiment that accompanying drawing 1 provides is the optical schematic diagram of the microscopical structure that do not need to change the color of observing image.In this embodiment, before the eyepiece 8 checking bias slice 7 is not set.
The embodiment that accompanying drawing 2 provides is the optical schematic diagram of microscopical structure of the color that can obtain the observation image.In this embodiment, before the eyepiece 8 of imaging optical path, be provided with checking bias slice 7.
Among above-mentioned two embodiment, micro-sample 5 on the back focal plane of condenser 4, position deviation between the two must not greater than the condenser focal distance f ± 5%; Equally, encoding board 3 is in the condenser front focal plane, its position deviation must not greater than the condenser focal distance f ± 5%.
Referring to accompanying drawing 3, encoding board 3 of the present invention near the ring-type intermediate frequency look district 11 in low frequency look district and near ring-type high frequency look district's 12 radial arrays in intermediate frequency look district, and constitutes an integral body with its clamping up and down by simple glass by the low frequency look district 9 of centre.Because the basic, normal, high frequency district of encoding board 3 has three kinds of different basic colors respectively, thereby when illumination path passes through sample, make image have the effect of colour, embossment and high contrast.In an embodiment, the color of sample and contrast are constant; In embodiment 2, by regulating the checking bias slice 7 in the imaging optical path, the just color of may command sample.
The advantage that the present invention is following owing to said structure has:
1, solved look lining micro-image high power objective coding hard problem;
2, under general condition, the object lens of variant multiple can use an encoding board jointly, make micro-The structure of mirror is simpler, uses convenient;
3, do not requiring under the prerequisite that changes color, can be without the checking bias slice before the eyepiece; Do not need polaroid on the encoding board simultaneously yet, make structure simpler.
4, novel color lining microscope according to claim 1 is characterized in that: micro-sample (5) on the back focal plane of condenser (4), position deviation between the two must not greater than the condenser focal distance f ± 5%; Encoding board (3) is in the condenser front focal plane, its position deviation must not greater than the condenser focal distance f ± 5%.

Claims (4)

1, a kind of novel color lining microscope, comprise illumination path that electric light source (1), collecting lens (2), condenser (4) constitute and the imaging optical path that constitutes by object lens (6), eyepiece (8), it is characterized in that: in illumination path, encoding board (3) is set on the front focal plane of condenser (4), the back focal plane of condenser overlaps with microscopical object plane, and sample (5) is on the back focus of condenser.
2, novel color lining microscope according to claim 1 is characterized in that: at the preceding checking bias slice (7) that is provided with of the eyepiece (8) of imaging optical path.
3, novel color lining microscope according to claim 1, it is characterized in that: encoding board (3) near the ring-type intermediate frequency look district (11) in low frequency look district and near ring-type high frequency look district (12) radial array in intermediate frequency look district, and constitutes an integral body with its clamping up and down by simple glass by the low frequency look district (9) of centre.
4, novel color lining microscope according to claim 1 is characterized in that: micro-sample (5) on the back focal plane of condenser (4), position deviation between the two must not greater than the condenser focal distance f ± 5%; Encoding board (3) is in the condenser front focal plane, its position deviation must not greater than the condenser focal distance f ± 5%.
CN01108514A 2001-06-08 2001-06-08 Color lining microscope Expired - Fee Related CN1121624C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN01108514A CN1121624C (en) 2001-06-08 2001-06-08 Color lining microscope

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN01108514A CN1121624C (en) 2001-06-08 2001-06-08 Color lining microscope

Publications (2)

Publication Number Publication Date
CN1321904A CN1321904A (en) 2001-11-14
CN1121624C true CN1121624C (en) 2003-09-17

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN01108514A Expired - Fee Related CN1121624C (en) 2001-06-08 2001-06-08 Color lining microscope

Country Status (1)

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CN (1) CN1121624C (en)

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Publication number Publication date
CN1321904A (en) 2001-11-14

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C06 Publication
PB01 Publication
C14 Grant of patent or utility model
GR01 Patent grant
C56 Change in the name or address of the patentee

Owner name: CHONGQING NORMAL COLLEGE

Free format text: FORMER NAME OR ADDRESS: WANG HUA

CP03 Change of name, title or address

Address after: 400047 No. 12 Chen Cheng Road, Shapingba District, Chongqing

Patentee after: Chongqing Normal College

Address before: 400047 Chongqing normal college, No. 12 Chen Cheng Road, Shapingba District, Chongqing

Patentee before: Wang Hua

C19 Lapse of patent right due to non-payment of the annual fee
CF01 Termination of patent right due to non-payment of annual fee