CN1048556C - False-colour optical stereomicroscope device and method thereof - Google Patents
False-colour optical stereomicroscope device and method thereof Download PDFInfo
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- CN1048556C CN1048556C CN 97108464 CN97108464A CN1048556C CN 1048556 C CN1048556 C CN 1048556C CN 97108464 CN97108464 CN 97108464 CN 97108464 A CN97108464 A CN 97108464A CN 1048556 C CN1048556 C CN 1048556C
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Abstract
The present invention relates to a technology of optical information processing, particularly to a stereoscopic vision false color optical stereomicroscope device and a method thereof. The present invention utilizes illumination of a point light source to reform a mechanical cylinder of a stereomicroscope. A color separation optical grating, a Fourier transformation lens and a four-dimensional adjustable rectangular filter are arranged, which forms a deformed white light image information processing system. Therefore, the direct observation for a false color stereoscopic vision image modulated according to the oblique illumination black and white gray gradation and the phase change rate for an object microstructure is realized on the stereomicroscope. The present invention is applied to biologic optical stereomicroscopes, metallic phase optical stereomicroscopes, polarized light optical stereomicroscopes, etc. The present invention does not need to be dyed or specially treated, a stereoscopic vision false color image of a real pattern of a microscopic sample can be observed or shot in real time by the present invention.
Description
The present invention relates to optical information processing technique, particularly a kind of false-colour optical stereomicroscope device.
In existing optical microscope display technique,, only do not observe having of its colored microstructure: the virtual-colorizing technique of polarisation method, fluorescence method, dark field method, color seperation, interference contrast method and micro-egative film etc. with pure method of optics to sample dyeing and special processing.These methods respectively possess some good points, but mostly or to sample certain restriction are arranged, or can not Real Time Observation, or are of limited application.
The object of the present invention is to provide the optical microphotograph lens device of a kind of directly Real Time Observation by object black and white gray scale and the colored stereo image of the microstructural vacation of phase change rate feature modulated object.
The objective of the invention is to be achieved through the following technical solutions: with general universal optical microscope is main frame, adopt spot light, and satisfy Kohler illumination or critical illumination condition, pack in microscope machinery tube color separation grating, Fourier transformation lens and adjustable square hole wave filter, the white light picture information that forms a kind of distortion is handled real-time playback system.The frequency plane of Fourier transformation lens and microscope aperture diaphragm conjugation, frequency plane are placed adjustable square hole wave filter, and the object lens imaging surface is placed the color separation grating.Adjustable square hole wave filter is composed the filtering of carrying out in various degree to the one-level of the different wave length of process color separation grating color separation, what the part primary spectrum of a certain color wavelength by the little bore edges of adjustable square hole wave filter was become resembles corresponding to oblique illumination, and whole primary spectrums of another color wavelength by aperture central authorities are corresponding to vertical illumination, thereby cause the false colored stereo image of black and white gray-level difference and phase change rate modulation when producing by the object oblique illumination on the imaging surface in the end.
The invention will be further described below in conjunction with accompanying drawing:
Fig. 1 is for implementing a kind of microscope imaging index path of the present invention;
Fig. 2 is the component drawings of four-dimensional adjustable square hole wave filter.
Shown in Figure 1, S is a pointolite, and L1, L2 are the condenser groups, and L3 is a microcobjective, and L4 is the Fourier transformation lens, and L5 is an eyepiece; P1 is a field stop, and P2 is an aperture diaphragm, and P3 is an object plane, and P4 is the object lens back focal planes, and P5 is object lens imaging surface, placement color separation grating P5 ', and P6 is frequency plane, places adjustable square hole wave filter P6 ', and P7 is the imaging surface of Fourier transformation lens.Wherein light source S and aperture diaphragm P2, object lens back focal plane P4, frequency plane P6 are mutual conjugation, and the imaging surface P7 of field stop P1 and object plane P3, object lens imaging surface P5, Fourier transformation lens is mutual conjugation.
White point light source S satisfies Kohler illumination or critical illumination condition, its light that sends is through collector lens group L1, the L2 collimation is that the directional light vertical irradiation is on the microscopic sample that is positioned over object plane P3, after microcobjective L3 amplifies, imaging is on object lens imaging surface P5, the color separation grating P5 ' that place on the P5 plane is a transmission phase-type grating, and first-order diffraction spectrum there is higher diffraction efficiency, it modulates color separation with image, go up the adjustable square hole wave filter P6 ' filtering of placing through the frequency plane P6 of Fourier transformation lens L4 again and select look, imaging is in the P7 plane then, but like this by eyepiece L5 just Direct observation (or shooting) to the false colored stereo image of object microstructure.Distance between wherein above-mentioned color separation grating P5 ' place object lens imaging surface P5 and Fourier transformation lens L4 object space principal plane is 2 times of this Fourier transformation focal length of lens.
Fig. 2 is a kind of assembly that constitutes the adjustable rectangle aperture of spectrum face wave filter, and this wave filter rectangle aperture vertically is formed by stacking by two thin slice H1, H2 that have wedge-shaped slot.Can make thin slice H1 and H2 move the adjusting that can realize rectangle aperture size and position at the orthogonal both direction in frequency plane P6 upper edge respectively through micromatic setting, and whole filter P6 ' can carry out axial adjustment and rotate regulating in microscopical machinery tube, thereby reaches the four-dimensional adjustable purpose of wave filter rectangle aperture.
A concrete application example of the present invention is as follows: an amplitude type plane holographic transmission grating (30/millimeter) is bleached, making it to become the relief type phase grating is placed on the object plane P3, be difficult to tell this grating fringe and striped pattern at common biological microscopically, but, (with XSB-03 type biological microscope is main frame with the colored microscopie unit of stereoscopic vacation provided by the invention and method thereof, bromine tungsten filament lamp (12V, 50W) or high-pressure sodium lamp (50W) make light source, light source pin hole (mm) is observed, not only can clearly differentiate grating fringe, the pattern of grating fringe and part strip flaw are also high-visible, and the grating fringe bossing of embossment demonstrates different colors with sunk part, its color is relevant with the logical optical wavelength range of wave filter rectangle aperture, or be the cycle striped of bluish-green interval multi-color cord, or be the cycle striped of yellow red interval multi-color cord.The biologic slice of observing other no dyeing with this devices and methods therefor also can be observed the false colored stereo image of section biology microscope tissue.
Experimental results show that: the colored microscopie unit of stereoscopic vacation of the present invention, but the real topography of Real Time Observation or shot object microstructure provides more information for deliberation, also can give prominence to high-frequency information, and a certain feature of object microstructure is carried out special project research.Because the aperture and the aperture diaphragm conjugation of adjustable square hole wave filter also can play the effect of regulating field depth in this device; By regulating the position of wave filter aperture on frequency plane, can make false colored microscopic image present different background colors and help improving colored resolution when observing; Utilize these characteristics simultaneously, also can play the effect of blocking-up filter plate at the rectangle aperture of fluorescent microscope median filter, and adjustable continuously; If use polarized illumination instead, then can utilize the nonlinear relationship of color separation diffraction efficiency of grating and polarized light direction of vibration to study the characteristic of anisotropy object.The colored microscopie unit volume of this stereoscopic vacation is little, light path is simple, cost is low, can be applicable to optical microscopes such as biology, metallographic, polarisation, has to use widely and promotional value.
Claims (3)
1, a kind of false-colour optical stereomicroscope device, it is characterized in that utilizing pointolite S illumination, the following assembly of in the machinery tube of optical microscope, packing into: the color separation grating (P5 '), Fourier transformation lens (L4) and adjustable square hole wave filter (P6 '), wherein color separation grating (P5 ') is on the imaging surface (P5) of microcobjective, the distance of its object space principal plane of Fourier transformation lens (L4) and microcobjective imaging surface (P5) is 2 times of this Fourier transformation lens (L4) focal length, and adjustable square hole wave filter (P6 ') be on the frequency plane (P6) of Fourier transformation lens (L4).
2, false-colour optical stereomicroscope device according to claim 1 is characterized in that color separation grating (P5 ') is transmission phase-type grating.
3, false-colour optical stereomicroscope device according to claim 1, the rectangle aperture that it is characterized in that adjustable square hole wave filter (P6 ') vertically is formed by stacking by two thin slice H1, H2 that have wedge-shaped slot, adjustable size, on frequency plane and the adjustable positions in the machinery tube, become four-dimensional adjustable square hole wave filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97108464 CN1048556C (en) | 1997-04-12 | 1997-04-12 | False-colour optical stereomicroscope device and method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97108464 CN1048556C (en) | 1997-04-12 | 1997-04-12 | False-colour optical stereomicroscope device and method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1164034A CN1164034A (en) | 1997-11-05 |
| CN1048556C true CN1048556C (en) | 2000-01-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 97108464 Expired - Fee Related CN1048556C (en) | 1997-04-12 | 1997-04-12 | False-colour optical stereomicroscope device and method thereof |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102230874A (en) * | 2011-03-23 | 2011-11-02 | 桂林优利特医疗电子有限公司 | Stepless zooming microscopic inspection device for full-automatic arena analyzer |
| CN107045189A (en) * | 2017-03-28 | 2017-08-15 | 湖南省天骑医学新技术股份有限公司 | A kind of light microscope based on spot light system |
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1997
- 1997-04-12 CN CN 97108464 patent/CN1048556C/en not_active Expired - Fee Related
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| CN1164034A (en) | 1997-11-05 |
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