CN100593114C - 乳粉蛋白质含量快速检测试剂盒及其制备方法 - Google Patents
乳粉蛋白质含量快速检测试剂盒及其制备方法 Download PDFInfo
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- CN100593114C CN100593114C CN200610056997A CN200610056997A CN100593114C CN 100593114 C CN100593114 C CN 100593114C CN 200610056997 A CN200610056997 A CN 200610056997A CN 200610056997 A CN200610056997 A CN 200610056997A CN 100593114 C CN100593114 C CN 100593114C
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- milk powder
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- 238000012360 testing method Methods 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 38
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 32
- OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 claims abstract description 72
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 37
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 37
- 239000012460 protein solution Substances 0.000 claims abstract description 33
- 238000001514 detection method Methods 0.000 claims abstract description 24
- 238000004519 manufacturing process Methods 0.000 claims abstract description 6
- 235000019624 protein content Nutrition 0.000 claims description 107
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
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- 241000669003 Aspidiotus destructor Species 0.000 claims description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 5
- 229940098773 bovine serum albumin Drugs 0.000 claims description 5
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 4
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- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 16
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 10
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- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
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- 238000004458 analytical method Methods 0.000 description 2
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- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 2
- 239000004327 boric acid Substances 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
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- 241000532370 Atla Species 0.000 description 1
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
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- 101001018064 Homo sapiens Lysosomal-trafficking regulator Proteins 0.000 description 1
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- 229920005479 Lucite® Polymers 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
样品编号 | A | B | C | D | E |
蛋白质含量(%) | 20 | 25 | 15-20 | 10-15 | 20 |
样品编号 | A | B | C | D | E |
蛋白质含量(%) | 20 | 20-30 | 15-20 | 10-15 | 20 |
样品编号 | A | B | C | D | E |
蛋白质含量(%) | 19.67 | 25.08 | 17.92 | 12.70 | 19.20 |
样品编号 | A | B | C | D | E |
蛋白质含量(%) | 20.2 | 24.0 | 17.2 | 13.2 | 19.7 |
方法名称 | 试剂盒法 | 凯氏定氮法 | 常规双缩脲法 |
仪器设备 | 少 | 多 | 较多 |
试剂 | 一种 | 多 | 较多 |
操作步骤 | 简单 | 复杂繁琐 | 较繁琐 |
所需时间 | 5-10分钟 | 3.5-4.5小时 | 1.5-2小时 |
人员要求 | 无特殊要求 | 专业人员 | 专业人员 |
场地要求 | 无特殊要求 | 实验室中 | 实验室中 |
结果 | 半定量 | 准确 | 较准确 |
现场检测 | 适合 | 不适合 | 不适合 |
Claims (1)
Priority Applications (1)
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CN200610056997A CN100593114C (zh) | 2006-03-07 | 2006-03-07 | 乳粉蛋白质含量快速检测试剂盒及其制备方法 |
Applications Claiming Priority (1)
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CN200610056997A CN100593114C (zh) | 2006-03-07 | 2006-03-07 | 乳粉蛋白质含量快速检测试剂盒及其制备方法 |
Publications (2)
Publication Number | Publication Date |
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CN101034065A CN101034065A (zh) | 2007-09-12 |
CN100593114C true CN100593114C (zh) | 2010-03-03 |
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CN200610056997A Expired - Fee Related CN100593114C (zh) | 2006-03-07 | 2006-03-07 | 乳粉蛋白质含量快速检测试剂盒及其制备方法 |
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CN (1) | CN100593114C (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021089707A1 (en) * | 2019-11-07 | 2021-05-14 | Nanonord A/S | A method of and a system for determining protein concentration in a selected material by nuclear magnetic resonance relaxometry |
Families Citing this family (8)
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CN102288597B (zh) * | 2011-05-12 | 2016-01-13 | 上海苏豪逸明制药有限公司 | 一种快速简便的多肽残留检测方法 |
CN102879538A (zh) * | 2012-08-26 | 2013-01-16 | 大连理工大学 | 一种测定乳粉中蛋白质含量的方法 |
CN102944550B (zh) * | 2012-10-15 | 2015-07-15 | 上海谱尼测试技术有限公司 | 面粉中过氧化钙的检测试剂盒及过氧化钙检测方法 |
CN103278469B (zh) * | 2013-05-24 | 2015-08-12 | 宁波美康生物科技股份有限公司 | 一种总蛋白检测试剂 |
CN104155292A (zh) * | 2014-06-19 | 2014-11-19 | 东力(南通)化工有限公司 | 一种米屈肼中间体3-(2,2,2-三甲基肼)丙酸甲酯甲基硫酸盐含量的检测方法 |
CN105486682A (zh) * | 2015-12-28 | 2016-04-13 | 东北农业大学 | 一种蛋白质快速检测试剂盒及其检测方法和应用 |
CN107179316A (zh) * | 2017-07-19 | 2017-09-19 | 山东大学 | 一种海参口服液快速鉴定试剂盒 |
CN108489972B (zh) * | 2018-03-02 | 2021-03-02 | 成都虹波钼业有限责任公司 | 一种快速测定溶液中低含量钼的方法 |
-
2006
- 2006-03-07 CN CN200610056997A patent/CN100593114C/zh not_active Expired - Fee Related
Non-Patent Citations (9)
Title |
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乳粉蛋白质数字图像检测方法的研究. 候彩云,孙建平.中国乳品工业,第33卷第5期. 2005 |
乳粉蛋白质数字图像检测方法的研究. 候彩云,孙建平.中国乳品工业,第33卷第5期. 2005 * |
双缩脲光度法快速测定乳品中的蛋白质. 杨柳桦,孙成均.现代预防医学,第32卷第8期. 2005 |
双缩脲比色法快速测定含乳固体饮料中蛋白质含量的探讨. 高素虹.广东卫生防疫,第25卷第3期. 1999 |
双缩脲比色法快速测定含乳固体饮料中蛋白质含量的探讨. 高素虹.广东卫生防疫,第25卷第3期. 1999 * |
双缩脲法测定乳中酪蛋白含量. 程涛,孙艳波,李健.中国乳品工业,第28卷第3期. 2000 |
双缩脲法测定乳中酪蛋白含量. 程涛,孙艳波,李健.中国乳品工业,第28卷第3期. 2000 * |
测定含乳固体饮料蛋白质含量的双缩脲比色法. 闫铁炜.内蒙古质量技术监督,第1期. 2002 |
测定含乳固体饮料蛋白质含量的双缩脲比色法. 闫铁炜.内蒙古质量技术监督,第1期. 2002 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021089707A1 (en) * | 2019-11-07 | 2021-05-14 | Nanonord A/S | A method of and a system for determining protein concentration in a selected material by nuclear magnetic resonance relaxometry |
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CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100303 |