CN100582625C - Hard matrix biochip drying tool - Google Patents
Hard matrix biochip drying tool Download PDFInfo
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- CN100582625C CN100582625C CN200810243472A CN200810243472A CN100582625C CN 100582625 C CN100582625 C CN 100582625C CN 200810243472 A CN200810243472 A CN 200810243472A CN 200810243472 A CN200810243472 A CN 200810243472A CN 100582625 C CN100582625 C CN 100582625C
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Abstract
The invention discloses a method for drying a hard-substrate biological chip and a drying tool thereof. The method comprises the following technical proposal: (1) the chip drying tool is started; temperature is set between 22 and 37 DEG C and is balanced for 8 to 15 minutes; (2) the hard-substrate biological chip is put to a mould of the drying tool; the centrifugal rotational speed is between 2,000 and 5,000 rmp; and the hard-substrate biological chip is subjected to centrifugal rotation for 5 to 40 minutes. The chip drying tool used in the method for drying the hard-substrate biological chip comprises a shell, an inner liner and a top cover; a motor is arranged between the shell and the inner liner; a rotating shaft of the motor runs through the liner wall of the inner liner, and extends into a chamber of the inner liner; the rotating shaft of the motor is connected with a rotary arm; two ends of the rotary arm are connected with a mould bracket respectively; a mould is arranged on each mould bracket; the mould is provided with densely distributed macropores; and the macropores run through the bottom of the mould through small pylomes running through the bottom. The periphery of the inner liner is provided with a temperature control module. Through the chip drying tool and the matched mould, the drying method utilizes centrifugal force to firstly fully throw closed liquid remained on the surface of the chip off the chip and fully evaporate less moisture remained on the surface of the chip under the centrifugal vacuum environment in order that the chip achieves the drying effect.
Description
Technical field
The present invention relates to a kind of hard substrate biological chips drywall tool, belong to the biochip technology field.
Background technology
The appearance of biochip technology, making in an experiment simultaneously, thousands of genes of parallel study and gene outcome become possibility.The advantage of biochip technology is high detection sensitivity, low sample consumption and high throughput analysis, biochip technology has caused global interest as the peculiar methods of gene expression analysis, sequence analysis, clinical diagnosis, drug discovery and drug screening.At present, hard substrate biological chips still is in laboratory stage, the hard substrate biological chips of industrial production do not occur, and traditional-handwork drying means production efficiency is lower, does not still have the drying means that is applied to the hard substrate biological chips industrial-scale production.
Summary of the invention
Technical problem to be solved by this invention is: a kind of drywall tool of realizing that the hard substrate biological chips industrial scale is produced is provided.
Technical scheme of the present invention is:
The chip drywall tool of hard substrate biological chips, comprise shell, inner bag, top cover, between shell and inner bag, be provided with motor, the courage wall of described inner bag is passed in the rotating shaft of described motor, stretches in the chamber of inner bag, connects turning arm in the rotating shaft of motor, the two ends of described turning arm respectively are connected with mould rack, mould is installed on the mould rack, and described mould is provided with densely covered macropore, and macropore runs through the bottom of mould by the small through hole with its bottom perforation.
Be provided with temperature control module around the inner bag; Be provided with sealing ring between shell and the top cover; Mould is provided with handle; The bottom of inner bag is provided with osculum; The bottom surface of inner bag is middle to tilting all around.
Beneficial effect: in the chip drywall tool of the present invention the mould that is placed on the motor shaft is arranged, during rotation, produce centrifugal force, and the bottom of mould is a hollow out, makes the residual liquid on hard substrate biological chips surface under action of centrifugal force, breaks away from chip surface, mould when rotated, above mould, produce vacuum environment, help the evaporation of moisture, help drying.Another favourable structure is to have loaded onto temperature control module at the centrifugal chamber casing periphery, can control the temperature in centrifugal zone effectively.With the bottom beveling of centrifugal chamber casing, do the discharge that helps waste liquid like this, be convenient to clean, guaranteed the interior clean environment of instrument, keep the cleaning of centrifugal chamber casing.The centrifugal mold of super large has improved batch process, has guaranteed production efficiency, integrate the heating and cooling module, guaranteed the stable of environment temperature, reduced the production difference between batch, this drywall tool can be finished dry run apace, the uniformity between again can retention tab and do not influence chip performance.
Description of drawings
Fig. 1 is the front view of chip drywall tool;
Fig. 2 is the vertical view of mould 7 among Fig. 1;
Fig. 3 is that the A-A of Fig. 2 is to view.
Among the figure:
| Numbering | Title | Numbering | Title |
| 1 | Top cover | 8 | Turning arm |
| 2 | Sealing ring | 9 | Osculum |
| 3 | |
10 | Motor |
| 4 | |
11 | Macropore |
| 5 | |
12 | |
| 6 | |
13 | |
| 7 | Mould |
The specific embodiment
The invention will be further described below in conjunction with embodiment and accompanying drawing thereof.
Embodiment 1
(1) opening chip drywall tool, 22 ℃ of design temperatures, balance 8 minutes;
(2) hard substrate biological chips is put into the mould of drywall tool, centrifugal rotary speed 2000rpm, centrifugal rotation 5 minutes.
Embodiment 2
(1) opening chip drywall tool, 26 ℃ of design temperatures, balance 12 minutes;
(2) hard substrate biological chips is put into the mould of drywall tool, centrifugal rotary speed 2800rpm, centrifugal rotation 12 minutes.
Embodiment 3
(1) opening chip drywall tool, 28 ℃ of design temperatures, balance 15 minutes;
(2) hard substrate biological chips is put into the mould of drywall tool, centrifugal rotary speed 3200rpm, centrifugal rotation 15 minutes.
Embodiment 4
(1) opening chip drywall tool, 30 ℃ of design temperatures, balance 12 minutes;
(2) hard substrate biological chips is put into the mould of drywall tool, centrifugal rotary speed 3600rpm, centrifugal rotation 18 minutes.
Embodiment 5
(1) opening chip drywall tool, 33 ℃ of design temperatures, balance 12 minutes;
(2) hard substrate biological chips is put into the mould of drywall tool, centrifugal rotary speed 4000rpm, centrifugal rotation 22 minutes.
(1) opening chip drywall tool, 37 ℃ of design temperatures, balance 10 minutes;
(2) hard substrate biological chips is put into the mould of drywall tool, centrifugal rotary speed 4200rpm, centrifugal rotation 25 minutes.
(1) opening chip drywall tool, 37 ℃ of design temperatures, balance 12 minutes;
(2) hard substrate biological chips is put into the mould of drywall tool, centrifugal rotary speed 4500rpm, centrifugal rotation 32 minutes.
Embodiment 8
(1) opening chip drywall tool, 37 ℃ of design temperatures, balance 15 minutes;
(2) hard substrate biological chips is put into the mould of drywall tool, centrifugal rotary speed 4800rpm, centrifugal rotation 38 minutes.
Embodiment 9
(1) opening chip drywall tool, 35 ℃ of design temperatures, balance 15 minutes;
(2) hard substrate biological chips is put into the mould of drywall tool, centrifugal rotary speed 2500rpm, centrifugal rotation 40 minutes.
(1) opening chip drywall tool, 37 ℃ of design temperatures, balance 15 minutes;
(2) hard substrate biological chips is put into the mould of drywall tool, centrifugal rotary speed 5000rpm, centrifugal rotation 40 minutes.
(1) opening chip drywall tool, 35 ℃ of design temperatures, balance 12 minutes;
(2) hard substrate biological chips is put into the mould of drywall tool, centrifugal rotary speed 4600rpm, centrifugal rotation 40 minutes.
(1) opening chip drywall tool, 37 ℃ of design temperatures, balance 8 minutes;
(2) hard substrate biological chips is put into the mould of drywall tool, centrifugal rotary speed 5000rpm, centrifugal rotation 30 minutes.
The chip drywall tool that uses in the drying means of above-mentioned hard substrate biological chips, include shell 4, inner bag 3, top cover 1, between shell 4 and inner bag 3, be provided with motor 10, the courage wall of described inner bag 3 is passed in the rotating shaft of motor 10, stretch in the chamber of inner bag 3, connect turning arm 8 in the rotating shaft of motor 10, the two ends of turning arm 8 respectively are connected with mould rack 6, mould 7 is installed on the mould rack 6, mould 7 is provided with densely covered macropore 11, and macropore 11 runs through the bottom of mould 7 by the small through hole 12 with its bottom perforation.
Be provided with temperature control module 5 around the inner bag 3.Be provided with sealing ring 2 between shell 4 and the top cover 1.Mould 7 is provided with handle 13.The bottom of inner bag 3 is provided with osculum 9.The bottom surface of inner bag 3 is middle to tilting all around.
During work, motor 10 rotations drive mould rack 6 rotations of turning arm 8 and the connection of turning arm 8 two ends, the mould of installing on the mould rack 67, together rotate together with the biochip in the macropore 11 that is placed in mould 7, during rotation, produced centrifugal force, and the bottom macropore 11 of mould 7 is by the bottom of small through hole 12 hollow out moulds 7, make the residual liquid of biochip surface under action of centrifugal force, break away from chip surface.By mould when rotated, the vacuum environment that produces above mould helps the evaporation of moisture, helps drying.At the temperature control module 5 that the centrifugal chamber casing periphery is loaded onto, can control the temperature in centrifugal zone effectively.With the bottom beveling of centrifugal chamber casing, do the discharge that helps waste liquid like this, be convenient to clean, guaranteed the interior clean environment of instrument, keep the cleaning of centrifugal chamber casing.
Table 1 is the part of detecting result of embodiment of the invention 1-12
The raw material that is used in the table 1 test is respectively:
Ankyrin: AFP (Alpha-Fetoprotein) antibody (the rich match in Henan biological production)
Signal antibody: horseradish peroxidase-labeled AFP antibody (the rich match in Henan biological production)
BSA:Roche company produces
Used general chemical reagent is analyzes pure rank
Reaction washing lotion: 0.1mol/l Tris-Hcl (biological production of U.S. season of Shanghai)
Luminous substrate: SuperSignal ELISA Femto Maximum Sensitivity Substrate (U.S. pierce production)
Centrifugal dryer: produce for my company designs voluntarily voluntarily
With the specking spotting robot of the U.S. biodot company ankyrin is put after surface of glass slide by the 4*12 array way during test, read signal, analyze through reaction.
Table 1: test result
Claims (6)
1, a kind of chip drywall tool of hard substrate biological chips, comprise shell (4), inner bag (3), top cover (1), it is characterized in that: between shell (4) and inner bag (3), be provided with motor (10), the courage wall of described inner bag (3) is passed in the rotating shaft of described motor (10), stretch in the chamber of inner bag (3), connect turning arm (8) in the rotating shaft of motor (10), the two ends of described turning arm (8) respectively are connected with mould rack (6), mould (7) is installed on the mould rack (6), described mould (7) is provided with densely covered macropore (11), and macropore (11) runs through the bottom of mould (7) by the small through hole (12) with its bottom perforation.
2, chip drywall tool according to claim 1 is characterized in that: be provided with temperature control module (5) around the described inner bag (3).
3, chip drywall tool according to claim 1 is characterized in that: be provided with sealing ring (2) between described shell (4) and the top cover (1).
4, chip drywall tool according to claim 1 is characterized in that: described mould (7) is provided with handle (13).
5, chip drywall tool according to claim 1 is characterized in that: the bottom of described inner bag (3) is provided with osculum (9).
6, chip drywall tool according to claim 1 is characterized in that: the bottom surface of described inner bag (3) is middle to tilting all around.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810243472A CN100582625C (en) | 2008-12-19 | 2008-12-19 | Hard matrix biochip drying tool |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810243472A CN100582625C (en) | 2008-12-19 | 2008-12-19 | Hard matrix biochip drying tool |
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| Publication Number | Publication Date |
|---|---|
| CN101435655A CN101435655A (en) | 2009-05-20 |
| CN100582625C true CN100582625C (en) | 2010-01-20 |
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| CN200810243472A Active CN100582625C (en) | 2008-12-19 | 2008-12-19 | Hard matrix biochip drying tool |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102252960B (en) * | 2011-04-21 | 2013-02-20 | 西北工业大学 | Device and method for measuring adhesive force between objects |
| CN102261825A (en) * | 2011-07-25 | 2011-11-30 | 李家凌 | Drying centrifuge |
| CN102814294B (en) * | 2012-06-19 | 2015-02-18 | 江苏三联生物工程有限公司 | Cleaning and drying device for biological chip |
| CN103206838B (en) * | 2013-04-02 | 2014-12-10 | 海博瑞恩电子科技无锡有限公司 | Electrode core drying device and method |
| CN104930837A (en) * | 2014-03-19 | 2015-09-23 | 上海空间电源研究所 | System and method for efficiently drying zinc-silver battery cathode pole piece |
| CN114353476A (en) * | 2022-01-04 | 2022-04-15 | 江西中弘晶能科技有限公司 | Method for improving drying of monocrystalline silicon wafer |
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| CN101435655A (en) | 2009-05-20 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Hard matrix biochip drying tool Effective date of registration: 20190726 Granted publication date: 20100120 Pledgee: Agricultural Bank of China Limited by Share Ltd Wuxi science and Technology Branch Pledgor: Jiangsu Sanlian Bioengineering Co., Ltd. Registration number: 2019990000792 |
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Date of cancellation: 20200810 Granted publication date: 20100120 Pledgee: Agricultural Bank of China Limited by Share Ltd. Wuxi science and Technology Branch Pledgor: SUNLANT BIOLOGICAL ENGINEERING Co.,Ltd. Registration number: 2019990000792 |
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Address after: No. 35-305, Changjiang South Road, Xinwu District, Wuxi, Jiangsu 214111 Patentee after: Jiangsu Sanlian Bioengineering Co.,Ltd. Address before: A science and Technology Park Comprehensive incubator building Xin'an District Road 214101 Jiangsu Xingyuan University City of Wuxi Province, first floor Patentee before: SUNLANT BIOLOGICAL ENGINEERING CO.,LTD. |
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