CN100574789C - A kind of promotion wound healing, the synulotic external Chinese medicine of minimizing and preparation method - Google Patents
A kind of promotion wound healing, the synulotic external Chinese medicine of minimizing and preparation method Download PDFInfo
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Abstract
The invention discloses a kind of promotion wound healing, reduce synulotic external Chinese medicine and preparation method thereof, the crude drug that makes this medicine is the compound compatibility of Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Lignum Dalbergiae Odoriferae, its prescription also has Galla Chinensis, the effective ingredient that extracts respectively with the different solvent of three classes as active ingredient, be aided with the acceptable pharmaceutic adjuvant and make the multiple external preparation of being convenient to clinical use.Preparation method of the present invention comprises the extraction and the preparation of effective ingredient: be solvent with water, ethanol and vegetable oil respectively, extract the effective ingredient (be called for short water extract, ethanol extract and oil and carry thing) of prescription, the substrate and the appropriate vessel that are aided with wetting agent, film former, antibacterial, emulsifying agent, thickening agent and gel, membrane, liniment and ointment etc. are made the external preparation that can supply selection of clinical to use.Medicine of the present invention is carried thing as active ingredient with water extract, ethanol extract and oil, all has significant promotion wound healing, reduces synulotic effect.
Description
Technical field
The present invention relates to a kind of Chinese prescription of external, especially a kind ofly be used to promote wound healing, reduce synulotic external Chinese medicine and this external preparation method of Chinese medicine thereof.
Background technology
Wound, operation etc. are all multifactorly all can to form wound, takes the necessary measures to promote wound healing, reduce cicatrization, has become an important subject in repair in trauma field.Both at home and abroad the world of medicine has carried out the safe and effective approaches and methods that long-term a large amount of experimentation that is deep into cell, molecule and gene level from tissue can shorten the damage phase in the hope of searching out, promote wound healing.
Be used at present promote that the method for wound healing mainly contains:
1, reduces the factor that influences wound healing as far as possible;
2, laser;
3, engineered application;
4, the application of biological engineering material etc. can increase as the chitin that extracts that fibroblast assembles and collagen is synthetic from dried small shrimps, Carapax Eriocheir sinensis.
The research of said method, application greatly improve and have promoted the content of repair in trauma, wound healing has been played facilitation significantly, but because wound healing is an extremely complicated biochemical process, said method respectively has superiority, also there are in various degree deficiency and defective simultaneously, lack a kind of gratifying method.
In the traditional Chinese medicine and pharmacy of motherland, the repair in trauma content is very abundant, has formed the theoretical system that shows unique characteristics, and has accumulated valuable clinical experience.In line with the principle of " hemostasis analgesic therapy, expelling the stasis for the new ", adopt the method that must use of blood circulation promoting and blood stasis dispelling, improved cure rate and function serviceability rate that skin trauma is repaired.The relevant proved recipe of report is more at present, and prescription is formed comparatively complexity, also lacks necessary pharmacological agent research, and curative effect is still needed and further verified.Therefore, screening has the Chinese prescription of the active treatment effect of stage construction, many target spots to repair in trauma, and is made into and is fit to safe, efficient modern medicines clinical practice, that meet national standard, will be the emphasis of this area research from now on.
Summary of the invention
The objective of the invention is to, provide a kind of and can promote wound healing, the synulotic external Chinese medicine of minimizing and preparation method thereof.The present invention is be guidances with the traditional Chinese medicine of China " hemostasis analgesic therapy, expelling the stasis for the new " theory, screens to have the Chinese medicine composition prescription of pretending usefulness, takes different extraction process extraction Effective Components of Chinese Herb and makes suitable exterior-applied formulation.Said preparation hemostasis effectively, antiinflammatory, exudation, shorten wound healing time, promote wound healing, reducing scar forms, integrate hemostasis, antiinflammatory, short more, effect such as desalination scar, for trauma patient provide a kind of safe, convenient, effectively promote wound healing, reduce synulotic new way.
In order to realize above-mentioned task, the present invention takes following technical solution:
A kind ofly be used to promote wound healing, reduce synulotic external Chinese medicine, it is characterized in that the Chinese medicine ingredients that makes this medicine comprises Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Lignum Dalbergiae Odoriferae, its ratio is Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae=(10-50): (5-50): (5-50).
Described crude drug also has Galla Chinensis, and the ratio of Galla Chinensis and other crude drug is Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae: Galla Chinensis=(10-50): (5-50): (5-50): (5-30).
Said external Chinese medicine is that all crude drug in the above-mentioned prescription are extracted effective ingredient as active ingredient with proper method, add proper auxiliary materials such as an amount of wetting agent, film former, antibacterial, emulsifying agent, excipient again and can make gel, membrane, liniment, ointment, spray and ointment etc. respectively, contain crude drug 5%～50% in its preparation.
Above-mentioned external preparation method of Chinese medicine, it is characterized in that, get crude drug by prescription and decoct with water secondary, each 1 hour, filter the back and merge twice filtrate, place after being concentrated into certain volume, the filtering precipitation is as water extract, add various pharmaceutic adjuvants then, packing can make gel, membrane, liniment, ointment or spray.
Perhaps, with crude drug, place hermetic container by prescription, with certain density soak with ethanol 5-7 day, the reuse soak with ethanol is extracted once behind the leaching solution, merges filtrate twice, control volume messenger drug substrate concentration remains in the proper range, is sub-packed in container after stirring evenly and promptly gets tincture or liniment.
Perhaps, vegetable oil is heated to about 180 ℃, adds crude drug by prescription, frying is filtered to sallow, adds Cera Flava in the fluid, stirs fusing, puts cold back packing.
The preparation of gel, membrane, liniment, ointment, spray and ointment is respectively:
A. the preparation of gel:
By the prescription weighting raw materials, decoct with water secondary, each 1 hour, filter the back and merge twice filtrate, cool after being concentrated into certain volume, filtering precipitates as the Chinese medicine water extract;
Other gets an amount of carbomer or other conventional gel-type vehicle is sprinkled in an amount of purified water, treat its natural swelling after, add an amount of glycerol, ethyl hydroxybenzoate and above-mentioned Chinese medicine water extract, constantly stir and drip triethanolamine number droplet down, be stirred to and coagulate the back packing promptly;
B. the preparation of membrane:
By the prescription weighting raw materials, decoct with water secondary, each 1 hour, filter the back and merge twice filtrate, cool after being concentrated into certain volume, filtering precipitates as the Chinese medicine water extract;
Other gets an amount of polyvinyl alcohol or other conventional membrane substrate is sprinkled in an amount of purified water, placement is spent the night, after treating its natural swelling, heat the dissolving after-filtration in the water-bath, add an amount of glycerol, ethyl hydroxybenzoate and above-mentioned Chinese medicine water extract, be incubated 30 minutes, be poured on bright and clean, the smooth flat board, pushing becomes certain thickness thin film, dry rear demoulding, packing gets final product;
C. the preparation of liniment:
By the prescription weighting raw materials, decoct with water secondary, each 1 hour, filter the back and merge twice filtrate, cool after being concentrated into certain volume, filtering precipitates as the Chinese medicine water extract;
Other gets an amount of polyvinyl alcohol or other conventional liniment substrate is sprinkled in an amount of purified water, and placement is spent the night, treat its natural swelling after, heat in the water-bath, the dissolving after-filtration adds an amount of glycerol, ethyl hydroxybenzoate and Chinese medicine water extract, stirs evenly the back packing promptly;
D. the preparation of spray:
By the prescription weighting raw materials, decoct with water secondary, each 1 hour, filter the back and merge twice filtrate, cool after being concentrated into certain volume, filtering precipitates as the Chinese medicine water extract;
In the Chinese medicine water extract, add an amount of glycerol, ethyl hydroxybenzoate, stir evenly the back fill in automiser spray promptly;
E. ointment preparation:
By the prescription weighting raw materials, decoct with water secondary, each 1 hour, filter the back and merge twice filtrate, to place after being concentrated into certain volume, filtering precipitates as the Chinese medicine water extract;
Add an amount of glycerol, ethyl hydroxybenzoate and, be heated to 70 ℃～85 ℃ at the Chinese medicine water extract, obtain aqueous-phase material as the triethanolamine of emulsifying agent;
Other gets an amount of stearic acid, vaseline, octadecanol, lanoline, is heated to consistently with the aqueous-phase material temperature, obtains the oil phase thing;
Under agitation aqueous-phase material is slowly added in the oil phase thing, be stirred to along same direction and coagulate promptly.
Described Chinese medicine water extract also can adopt another kind of method to obtain, and reclaims the aqueous solution that the etoh solvent of Chinese medicine ethanol extract obtains that is:, and concrete grammar is:
By the prescription weighting raw materials, place hermetic container, with certain density soak with ethanol 5-7 day, behind the leaching solution filtering residue reuse soak with ethanol is extracted once, merge filtrate twice, reclaim ethanol and extremely do not have the alcohol flavor, and be concentrated into certain volume promptly.
The preparation of described tincture or liniment is respectively:
A. the preparation of tincture:
By the prescription weighting raw materials, with certain density soak with ethanol 5-7 day, the reuse soak with ethanol is extracted once behind the leaching solution, merges filtrate twice, controls volume messenger drug substrate concentration and remains in the proper range, is sub-packed in after stirring evenly in the hermetic container promptly;
Perhaps, by the prescription weighting raw materials, with certain density alcohol heating reflux secondary, each 1 hour, filter, merge filtrate twice, control volume messenger drug substrate concentration and remain in the proper range, be sub-packed in after stirring evenly in the hermetic container promptly;
B. the preparation of liniment:
By the prescription weighting raw materials, with certain density soak with ethanol 5-7 day, the reuse soak with ethanol is extracted once behind the leaching solution, merges filtrate twice, controls volume messenger drug substrate concentration and remains in the proper range, is sub-packed in after stirring evenly in the appropriate vessel promptly.
The preparation of described ointment is:
Vegetable oil is heated to about 180 ℃, adds crude drug by prescription, frying is filtered to sallow, adds Cera Flava or other suitable thickening agent in the fluid, stirs fusing, puts cold back packing.
Promotion wound healing of the present invention, the synulotic external Chinese medicine of minimizing, crude drug wherein all has good anastalsis.Pseudobulbus Bletillae (Rhizoma Bletillae), Galla Chinensis hemostasis convergence promote wound healing; Radix Angelicae Sinensis, Lignum Dalbergiae Odoriferae nourish blood and blood, and the analgesic therapy of promoting the circulation of blood dissipating blood stasis improves local microcirculation, strengthen local blood fortune, improve wound nutrition, and accelerating wound healing makes only and the not stasis of blood of blood, promotes the absorption of local inflammation exudate.Because of it has circulation of qi promoting and blood effect again,, also can bring into play facilitation preferably to the reparation of local wound.Therefore, above-mentioned Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Lignum Dalbergiae Odoriferae share or all medicines of Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Lignum Dalbergiae Odoriferae and Galla Chinensis share, and then all have the hemostasis analgesic therapy, detoxify and hold back the effect of skin ulcer.
The present invention is in conjunction with the traditional theory and the modern pharmacological research theory of Chinese medicine, be the water soluble ingredient in the solvent extraction prescription, pure soluble components and oil-soluble composition with water, ethanol and vegetable oil respectively, water extract mainly comprises compositions such as alkaloid salt, glycosides, water-soluble organic acid, tannin, saccharide, aminoacid; The Chinese medicine ingredients of ethanol extraction comprises many hydrophilic compositions that isolating protein, phlegmatic temperament, pectin, starch and part polysaccharide etc. are outer, be insoluble in the low-polarity component of water in addition, the oil-soluble composition then comprises liposoluble constituents such as free alkaloid, volatile oil and many aromatic compounds.
The zoopery result shows, the water extract of Chinese medicine involved in the present invention, ethanol extract and oil are carried thing all has significant curative effect to a plurality of links in the skin wound healing process, therefore, with water extract, ethanol extract and oil carry thing as active ingredient, be aided with the acceptable pharmaceutic adjuvant, can make suitable external preparations respectively.
Description of drawings
Fig. 1 is a preparation method flow sheet of the present invention.
Be described in further detail below in conjunction with the external Chinese medicine of the present invention preparation and preparation method thereof flow sheet.
The specific embodiment
External Chinese prescription involved in the present invention is made up of Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Lignum Dalbergiae Odoriferae three flavor medicines, and its ratio is Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae=(10-50): (5-50): (5-50).Perhaps
Chinese prescription is made up of Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Lignum Dalbergiae Odoriferae and Galla Chinensis four Chinese medicine, and its ratio is Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae: Galla Chinensis=(10-50): (5-50): (5-50): (5-30).
Pseudobulbus Bletillae (Rhizoma Bletillae) is a monarch drug in the Chinese prescription, have another name called give free of charge, the Pseudobulbus Bletillae (Rhizoma Bletillae), bitter sweet, cold, nontoxic, can astringing to arrest bleeding, detumescence and promoting granulation is held back skin ulcer.Cure mainly the bleeding due to a variety of causes such as spitting of blood, epistaxis, incised wound be hemorrhage.Modern pharmacology experimental results show that Pseudobulbus Bletillae (Rhizoma Bletillae) has good astringing to arrest bleeding, detumescence and promoting granulation function, is usually used in treating diseases such as gastric and duodenal ulcers, erosive gastritis, chronic skin infection, epidermis burn and scald.Can also improve wound macrophage quantity in addition, improve extracellular matrix collagen level, thereby improve the wound healing quality.
Radix Angelicae Sinensis and Lignum Dalbergiae Odoriferae are ministerial drugs, the Radix Angelicae Sinensis suffering, and temperature, nontoxic, the function hemostasis of regulating the flow of vital energy, the clots absorbing analgesic therapy curing mainly spitting of blood and spitting blood, traumatic injury, incised wound is hemorrhage.Have the effect of the tissue metabolism of improvement, can significantly delay amyotrophy; The Lignum Dalbergiae Odoriferae suffering, temperature, nontoxic, the function hemostasis of regulating the flow of vital energy, the clots absorbing analgesic therapy curing mainly spitting of blood and spitting blood, traumatic injury, incised wound is hemorrhage.The Compendium of Material Medica record can " be treated folding and hinder incised wound, hemostasis analgesic therapy, detumescence and promoting granulation." row such as Lignum Dalbergiae Odoriferae end, Galla Chinensis end, copper end transfer and apply, and control sword or traumatic injury, haemophilia (" 101 choosing side ").
Galla Chinensis is an adjuvant drug, and acid is flat, nontoxic.Astringe the lung astringing intestine to stop diarrhea hemostasis of function.Surgery is usually used in treating various skin infection erosions, does not close up for a long time, and incised wound is hemorrhage etc.Have stronger antibacterial action, and can obviously be suppressed to the in-vitro multiplication rate of fibrocyte (Fb), and strong to the normal skin Fb of effect of hypertrophic cicatrix Fb, be significant amount-effect dependence.Galla Chinensis can obviously influence fibroblast form and ultrastructure, suppresses the propagation of fibroblasts from hypertrophic scars and synthesizing of collagen protein.
All medicines in the prescription all have good anastalsis.Pseudobulbus Bletillae (Rhizoma Bletillae), Galla Chinensis hemostasis convergence promote wound healing; Radix Angelicae Sinensis, Lignum Dalbergiae Odoriferae nourish blood and blood, and the analgesic therapy of promoting the circulation of blood dissipating blood stasis improves local microcirculation, strengthen local blood fortune, improve wound nutrition, and accelerating wound healing makes only and the not stasis of blood of blood, promotes the absorption of local inflammation exudate.Because of it has circulation of qi promoting and blood effect again,, also can bring into play facilitation preferably to local wound healing.Four medicines share, and then have the effect of hemostasis analgesic therapy, expelling the stasis for the new.
Fig. 1 has provided preparation technology's flow chart of the present invention.
With the preparation method (with gel is example) of water extract as the external preparation of active ingredient:
The first step: take by weighing all medicines of prescription, decoct with water secondary, each 1 hour, filter the back and merge twice filtrate, to place after being concentrated into certain volume, filtering precipitates as water extract.
Second step: get carbomer (or other gel-type vehicle) and be sprinkled in an amount of purified water, treat its natural swelling after, add glycerol, ethyl hydroxybenzoate and Chinese medicine water extract, constantly stir and drip triethanolamine down, be stirred to and coagulate the back packing promptly.
Ethanol in the ethanol extract is reclaimed, and also can obtain with water is the extract (method of directly extracting with water relatively, operate comparatively loaded down with trivial detailsly, cost increase but impurity is few, preparation stability is better) of solvent.Therefore, this extract can be made the water solublity external preparation according to the method described above.
With the preparation method (with tincture is example) of ethanol extract as the external preparation of active ingredient:
Take by weighing all medicines of prescription, with certain density soak with ethanol 5-7 day, the reuse soak with ethanol is extracted once behind the leaching solution, merges filtrate twice, and control volume messenger drug substrate concentration remains in the proper range, stirs evenly the back packing promptly.
Put forward the preparation method (with ointment is example) of thing with oil as the external preparation of active ingredient:
Vegetable oil is heated to about 180 ℃ in advance, adds crude drug by prescription, frying is filtered to sallow, adds Cera Flava in the fluid, and stirring and dissolving is put cold back packing.
Of the present inventionly be used to promote wound healing, reduce synulotic external Chinese medicine, its distinguishing feature is:
(1) this repair in trauma prescription contains Chinese medicine Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Lignum Dalbergiae Odoriferae and Galla Chinensis, the total medical material amount that contains 5-50% (g/ml or g/g) in the preparation made from this prescription, and the proportion of composing of the medical material of respectively distinguishing the flavor of in the prescription is a Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae: Galla Chinensis=(10-50): (5-50): (5-50): (5-30);
Pseudobulbus Bletillae (Rhizoma Bletillae) is a monarch drug, and Radix Angelicae Sinensis, Lignum Dalbergiae Odoriferae are ministerial drug, is the topmost composition of this prescription with above-mentioned three medicines, and its proportion of composing is a Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae=(10-50): (5-50): (5-50).Being aided with other has Chinese medicine of repairing wound, promoting healing and will form new prescription and be used for such treatment of diseases.
(2) with water be extract solvent, be principal agent with the water soluble ingredient, or, be aided with acceptable auxiliary element such as wetting agent, film former, antibacterial etc. and can be made into dosage forms such as gel, membrane, liniment, spray to have reclaimed alcoholic acid ethanol extract as water solublity drug effect composition;
Based on liposoluble constituent, also the ethanol extract that contains some water soluble ingredient can be made into tincture, liniment etc. as active ingredient.
Be to extract solvent, comprise that with liposoluble constituent free alkaloid, volatile oil and many aromatic compounds are the main pharmacodynamics composition, can make ointment with the vegetable oil.
(3) external preparation method of Chinese medicine of the present invention comprises three classes, promptly to prepare with the water extract be the method (abbreviation method 1) of effective ingredient, be the method (abbreviation method 2) of effective ingredient with the ethanol extract and put forward the method that thing is an effective ingredient (abbreviation method 3) with oil that preparation method may further comprise the steps:
Method 1 (with the example that is prepared as of gel):
1. take by weighing all medicines of prescription, decoct with water secondary, each 1 hour, filter the back and merge twice filtrate, to place after being concentrated into certain volume, filtering precipitates as water extract.
2. getting carbomer (or other gel-type vehicle such as sodium carboxymethyl cellulose) in addition is sprinkled in an amount of purified water, after treating its natural swelling, the wetting agent that adds glycerol or other kind, as propylene glycol, the antiseptic of ethyl hydroxybenzoate or other kind is received as benzoic acid, and the Chinese medicine water extract, constantly stir down and drip triethanolamine, be stirred to and coagulate the back packing promptly.
Method 2 (with the example that is prepared as of tincture):
Take by weighing all medicines of prescription, with certain density soak with ethanol 5-7 day, filter, filtering residue reuse soak with ethanol is extracted once, filters, and merges filtrate twice, and control volume messenger drug substrate concentration remains in the proper range, stirs evenly the back packing promptly.
Method 3 (with the example that is prepared as of ointment):
Vegetable oil is heated to about 180 ℃, adds crude drug by prescription, frying is filtered to sallow, adds Cera Flava or other suitable thickening agent in the fluid, stirs fusing, puts cold back packing.
It below is the specific embodiment that the inventor provides.
Embodiment 1: the preparation gel
Prescription: Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae=30: 40: 20
Take by weighing all medicines of prescription, decoct with water secondary, each 1 hour, filter the back and merge twice filtrate, to place after being concentrated into certain volume, filtering precipitates as water extract.
Other gets carbomer (or other gel-type vehicle such as sodium carboxymethyl cellulose) and is sprinkled in an amount of purified water, treat its natural swelling after, add an amount of glycerol, ethyl hydroxybenzoate and Chinese medicine water extract, constantly stir and drip triethanolamine down, be stirred to and coagulate the back packing promptly.
Embodiment 1-1: different is that the ratio of prescription is adjusted into: Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis for this embodiment and embodiment 1: Lignum Dalbergiae Odoriferae=20: 40: 40, all the other are all with embodiment 1.
Embodiment 1-2: different is that the ratio of prescription is adjusted into: Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis for this embodiment and embodiment 1: Lignum Dalbergiae Odoriferae=10: 50: 40, all the other are all with embodiment 1.
Embodiment 1-3: different is that the ratio of prescription is adjusted into: Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis for this embodiment and embodiment 1: Lignum Dalbergiae Odoriferae=50: 25: 25, all the other are all with embodiment 1.
Embodiment 1-4: different is that the ratio of prescription is adjusted into: Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis for this embodiment and embodiment 1: Lignum Dalbergiae Odoriferae=30: 35: 35, all the other are all with embodiment 1.
Embodiment 1-5: different is that the ratio of prescription is adjusted into: Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae for this embodiment and embodiment 1: Galla Chinensis=30: 20: 20: 10, and all the other are all with embodiment 1.
Embodiment 1-6: different is that the ratio of prescription is adjusted into: Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae for this embodiment and embodiment 1: Galla Chinensis=30: 30: 30: 10, and all the other are all with embodiment 1.
Embodiment 1-7: different is that the ratio of prescription is adjusted into: Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae for this embodiment and embodiment 1: Galla Chinensis=20: 30: 30: 20, and all the other are all with embodiment 1.
Embodiment 1-8: different is that the ratio of prescription is adjusted into: Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae for this embodiment and embodiment 1: Galla Chinensis=30: 20: 20: 30, and all the other are all with embodiment 1.
Embodiment 1-9: different is that the ratio of prescription is adjusted into: Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae for this embodiment and embodiment 1: Galla Chinensis=40: 20: 20: 20, and all the other are all with embodiment 1.
Embodiment 1-10: different is that the ratio of prescription is adjusted into: Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae for this embodiment and embodiment 1: Galla Chinensis=45: 25: 25: 5, and all the other are all with embodiment 1.
Need to prove that present embodiment provides 11 kinds of embodiment, adjust, all can reach purpose of the present invention in the prescription scope that the present invention provides.
Embodiment 2: the preparation membrane
Need to prove, the membrane preparation causes with institute's dosage homogeneous that embodiment 1 provides various embodiment, and just the dosage form difference of preparation is enumerated in the present embodiment no longer one by one, adjust in the prescription scope that the present invention provides, all can reach purpose of the present invention.
Take by weighing all medicines of prescription, decoct with water secondary, each 1 hour, filter the back and merge twice filtrate, to place after being concentrated into certain volume, filtering precipitates as water extract.
Other gets polyvinyl alcohol (or other membrane substrate such as chitosan) and is sprinkled in an amount of purified water, placement is spent the night, after treating its natural swelling, heat the dissolving after-filtration in the water-bath, add an amount of glycerol, ethyl hydroxybenzoate and Chinese medicine water extract, be incubated 30 minutes, be poured on bright and clean, the smooth flat board, pushing becomes certain thickness thin film, dry rear demoulding, packing gets final product.
Embodiment 3: the preparation liniment
Need to prove, the liniment preparation causes with institute's dosage homogeneous that embodiment 1 provides various embodiment, and just the dosage form difference of preparation is enumerated in the present embodiment no longer one by one, adjust in the prescription scope that the present invention provides, all can reach purpose of the present invention.
Take by weighing all medicines of prescription, decoct with water secondary, each 1 hour, filter the back and merge twice filtrate, to place after being concentrated into certain volume, filtering precipitates as water extract.
Other gets polyvinyl alcohol (or other liniment substrate) and is sprinkled in an amount of purified water, and placement is spent the night, treat its natural swelling after, heat in the water-bath, the dissolving after-filtration, add an amount of glycerol, ethyl hydroxybenzoate and Chinese medicine water extract, stir evenly the back packing promptly.
Embodiment 4: the preparation spray
Need to prove, the spray preparation causes with institute's dosage homogeneous that embodiment 1 provides various embodiment, and just the dosage form difference of preparation is enumerated in the present embodiment no longer one by one, adjust in the prescription scope that the present invention provides, all can reach purpose of the present invention.
Take by weighing all medicines of prescription, decoct with water secondary, each 1 hour, filter the back and merge twice filtrate, to place after being concentrated into certain volume, filtering precipitates as water extract.
In medicinal liquid, add adjuvants such as an amount of glycerol, ethyl hydroxybenzoate, stir evenly the back fill in automiser spray promptly.
Embodiment 5: the preparation ointment
Need to prove, the ointment preparation causes with institute's dosage homogeneous that embodiment 1 provides various embodiment, and just the dosage form difference of preparation is enumerated in the present embodiment no longer one by one, adjust in the prescription scope that the present invention provides, all can reach purpose of the present invention.
Take by weighing all medicines of prescription, decoct with water secondary, each 1 hour, filter the back and merge twice filtrate, to place after being concentrated into certain volume, filtering precipitates as water extract.Add an amount of glycerol, ethyl hydroxybenzoate, emulsifying agent such as triethanolamine, be heated to 70-85 ℃, be water.
Other gets stearic acid, vaseline, octadecanol, lanoline etc., is heated to consistently with the water temperature, is oil phase.
Under agitation water is slowly added in the oil phase, be stirred to along same direction and coagulate promptly.
Embodiment 6: the preparation tincture
Need to prove, the tincture preparation causes with institute's dosage homogeneous that embodiment 1 provides various embodiment, and just the dosage form difference of preparation is enumerated in the present embodiment no longer one by one, adjust in the prescription scope that the present invention provides, all can reach purpose of the present invention.
Take by weighing all medicines of prescription, with certain density soak with ethanol 5-7 day, behind the leaching solution filtering residue reuse soak with ethanol is extracted once, merge filtrate twice, control volume messenger drug substrate concentration remains in the proper range, is sub-packed in after stirring evenly in the hermetic container promptly.
Embodiment 7: the preparation liniment
Need to prove, the liniment preparation causes with institute's dosage homogeneous that embodiment 1 provides various embodiment, and just the dosage form difference of preparation is enumerated in the present embodiment no longer one by one, adjust in the prescription scope that the present invention provides, all can reach purpose of the present invention.
Take by weighing all medicines of prescription, with certain density soak with ethanol 5-7 day, the reuse soak with ethanol is extracted once behind the leaching solution, merges filtrate twice, and control volume messenger drug substrate concentration remains in the proper range, is sub-packed in after stirring evenly in the appropriate vessel promptly.
Embodiment 8: the preparation ointment
Need to prove, the ointment preparation causes with institute's dosage homogeneous that embodiment 1 provides various embodiment, and just the dosage form difference of preparation is enumerated in the present embodiment no longer one by one, adjust in the prescription scope that the present invention provides, all can reach purpose of the present invention.
Vegetable oil is heated to about 180 ℃, adds crude drug by prescription, frying is filtered to sallow, adds Cera Flava or other suitable thickening agent in the fluid, stirs fusing, puts cold back packing.
Among the above-mentioned embodiment that provides, wherein, the water extract among embodiment 1, embodiment 2, embodiment 3, embodiment 4, the embodiment 5 all can adopt the resulting aqueous solution of ethanol extract behind the recovery ethanol to make active ingredient.That is:
Take by weighing all medicines of prescription,, behind the leaching solution filtering residue reuse soak with ethanol is extracted once, merge filtrate twice, reclaim ethanol to nothing alcohol flavor, and be concentrated into certain volume promptly with certain density soak with ethanol 5-7 day.
Experimental result:
Adopting external Chinese prescription of the present invention, is that the solvent extraction Effective Components of Chinese Herb obtains water extract, ethanol extract and oil and carries thing as the experiment medicine with water, ethanol and vegetable oil respectively, observes the facilitation of medicine to wound healing with the skin trauma rat model.The experiment content and the summary of results are as follows:
(1) experimental technique:
Get the SD rat, the male and female dual-purpose, routine is sloughed back wool behind the conventional raising 3d.Next day, in 1cm place, side, spinal column both sides, back, parallel spinal column was done the otch that is about 3cm respectively, reaches the flesh layer deeply under anesthesia, after the hemostasis, and 3-0 silk suture wound.
To test and be divided into four groups at random: negative control group, treatment component are that Chinese medicine water extract group (hereinafter to be referred as the water preparation group), Chinese medicine ethanol extract group (hereinafter to be referred as the tincture group), Chinese medicine oil are put forward thing group (hereinafter to be referred as the oil preparation group), and every group each 18, single cage is fed.Before medication every day earlier with iodine tincture alcohol disinfecting wound, each group external Chinese medicine extract respectively then, consumption 1.0ml/ wound is evenly smeared wound, every day secondary, to postoperative the 10th day; Negative control group (abbreviation matched group) is sew up wound directly, every day iodine tincture alcohol disinfecting wound, to postoperative the 10th day.
(2) experimental result:
1. ordinary circumstance:
All animal postoperative whole bodies are all right, do not have diarrhoea, and bad of refusing to eat etc. are answered, and are movable normal.
Postoperative 1,3 days: water preparation group, tincture group: wound is cleaning, and sepage is less, and the part wound has a little incrustation, and inflammatory reaction is light, and wound circumference does not have obvious redness; The oil preparation group: the wound incrustation, inflammatory reaction is lighter, and wound circumference does not have obvious redness; Matched group: the part wound has incrustation, and the part wound fluid is more, the wound circumference redness.
Postoperative 6 days: water preparation group, tincture group: epidermal area heals, does not have redness, and the part wound has lighter suture reaction; The oil preparation group: epidermal area heals substantially, does not have redness, and the part wound has suture reaction; Matched group: the most of incrustation of wound, wound circumference is red and swollen slightly, and the reaction of suture place is heavier.
Postoperative 9,14,28 days: water preparation group, tincture group: wound healing is good, does not have significantly healing line; The oil preparation group: wound healing is good, can see the healing line.Matched group: wound healing is good, visible significantly healing line.
2. the histology changes
2.1HE coloration result:
Postoperative 1 day, each is organized the surface and sees blood clot and inflammatory exudate, and epidermis is discontinuous, and inflammatory cell infiltrations such as visible more neutrophilic granulocyte, plasma cell, mononuclear cell in the skin corium of otch both sides are based on neutrophilic granulocyte.
Postoperative 3 days, water preparation group, tincture group: epidermal area heals substantially, visible newborn blood capillary and more fibroblast in the skin corium, inflammatory cell infiltration is less, inorganization internal hemorrhage in the skin corium.Oil preparation group epidermal area and skin corium do not heal, visible more fibroblast and inflammatory cell in the skin corium.Matched group epidermal area and skin corium do not heal, and visible part is organized internal hemorrhage in the skin corium, and fibroblast quantity is few, and inflammatory cell is more.
Postoperative 6 days: water preparation group, tincture group: epidermal area heals, and the part skin corium also heals, and visible a large amount of fibroblast is spindle shape in the skin corium, the loose and a spot of inflammatory cell of nucleus.Oil preparation group epidermal area heals substantially, and visible more fibroblast is spindle shape in the skin corium, and nucleus is bigger.The matched group epidermal area heals substantially, and fibroblast in the wound skin corium is spindle shape, and quantity is few, and is sparse, and nucleus is little, and more inflammatory cell is arranged.
Postoperative 9 days: each is organized wound and heals, visible a large amount of fibroblast and a large amount of new crude rubber original fibers in water preparation group, the tincture group skin corium.Visible more fibroblast and newborn collagen fiber in the oil preparation group skin corium.The above three groups of quantity of the fibroblast of matched group skin corium internal breeding are few, and newborn collagen is also less.
Postoperative 14,28 days: the cicatrix of matched group wound healing is bigger.Oil preparation group wound scar is less than matched group.Water preparation group, tincture group: cicatrix is little in the healing of wound.
2.2 influence to the blood capillary generation:
3,6,9 days after surgery, each treatment group group and matched group comparison wound surface capillary vessel number significantly increased, and significant difference (P＜0.05) is arranged, after modeling 14 days, except that matched group, the capillary vessel number of each treatment group is more preceding all to be reduced to some extent, but compares no difference of science of statistics (P＞0.05) between each group.The result shows that Chinese medicinal formulae of the present invention has the effect that promotes the generation of wound blood capillary, the local blood oxygen supply of increase, acceleration wound surface metabolism significantly, promotes wound healing.
Show capillary vascular counts in the 1:400 times of light microscopic visual field
Compare * p＜0.05, * * p＜0.01 with matched group; Compare #p＜0.05 with the oil preparation group
2.3 influence to fibroblastic growth:
3,6,9 days after surgery, the local fibroblast of each treatment group of Chinese medicine and matched group comparison wound significantly increased, and significant difference (P＜0.05) is arranged, and it is after the modeling the 9th day that fibroblast reaches the peak period.After modeling 14 days, the more preceding minimizing of fibroblast, but no difference of science of statistics (P＞0.05) relatively between each group.Show: external Chinese medicine of the present invention has obvious quickening fibroblasts proliferation, promotes the effect of wound healing.
Show fibroblast number in the 2:400 times of light microscopic visual field
Compare * p＜0.05, * * p＜0.01 with matched group; Compare #p＜0.05 with the oil preparation group
2.4 influence to hydroxyprolin levels in the wound granulation tissue:
OHP content no significant difference in 3 days wound surface granulation tissuies after modeling; 6 days, 9 days the time, each organizes all more preceding obvious rising of index value after the modeling, and each treatment group all is significantly higher than matched group (p＜0.05).Modeling 14 days, each group is all on a declining curve, compares no difference of science of statistics between group.Show that Chinese medicine of the present invention is significantly increased collagenation, promotes wound healing, reduces synulotic effect.
The influence of hydroxyprolin levels in the table 3 pair wound granulation tissue
Compare * p＜0.05 with matched group, #p＜0.05 is compared with the oil preparation group in * * p＜0.01
Conclusion:
1. external Chinese medicine of the present invention can obviously alleviate the wound surface inflammatory reaction, reduce and ooze out, and shortens wound healing time, reduces cicatrization.
2. external Chinese medicine of the present invention can obviously increase wound blood capillary quantity, promotes growth of fibroblasts, the state thereby the microcirculation stasis of blood stagnates, blood circulation promoting increases the supply of local blood oxygen, quickens the wound surface metabolism, promote blood capillary to generate fibroblasts proliferation and collagen deposition.
3. wound healing is the process of a complexity, and external Chinese medicine of the present invention all has therapeutical effect preferably to a plurality of links in the skin wound healing process.
Medicine of the present invention compared with prior art has following characteristics:
1. the present invention is on zooperal basis, with water extract of TCM, alcohol extract and oil carry thing as Effective component is made respectively the external preparation with wound repair effect of multiple different purposes, becomes phase-splitting To clearly, be convenient to quality control, also be convenient to patient's choice for use.
2. external application Chinese medicine of the present invention can act on a plurality of periods in the skin wound healing process and many Individual link: anti-inflammatory, imperviously go out, promote capillary regeneration, shorten wound thereby increase collagen generation etc. Healing time promotes wound healing, reduces scar and forms, and demonstrates omnibearing effect.
3. external application Chinese medicine of the present invention adopts clinical Chinese herbal medicine commonly used, and security is good.
Claims (5)
1, a kind ofly is used to promote wound healing, reduces synulotic external Chinese medicine, it is characterized in that the raw materials of effective components medicine and the part by weight thereof that make this medicine are Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae=10～50: 5～50: 5～50.
2, a kind ofly be used to promote wound healing, reduce synulotic external Chinese medicine, it is characterized in that the raw materials of effective components medicine and the part by weight thereof that make this medicine are Pseudobulbus Bletillae (Rhizoma Bletillae): Radix Angelicae Sinensis: Lignum Dalbergiae Odoriferae: Galla Chinensis=10～50: 5～50: 5～50: 5～30.
3, external Chinese medicine as claimed in claim 1 or 2, it is characterized in that, described external Chinese medicine is to be to extract the water soluble ingredient of solvent extraction as active ingredient with water, or to have reclaimed alcoholic acid ethanol extract as active ingredient, and add adjuvant and make gel, membrane, liniment, ointment or spray, contain active ingredient in each dosage form and be equivalent to 5%～50% of crude drug summation.
4, external Chinese medicine as claimed in claim 1 or 2, it is characterized in that, described external Chinese medicine is to be the liposoluble constituent that extracts solvent extraction with ethanol, the ethanol extract that also contains some water soluble ingredient is as active ingredient, make tincture or liniment, contain active ingredient in the dosage form and be equivalent to 5%～50% of crude drug summation.
5, external Chinese medicine as claimed in claim 1 or 2, it is characterized in that, described external Chinese medicine is to be to extract the oil-soluble composition of solvent extraction as active ingredient with the vegetable oil, and adds adjuvant and make ointment, contains active ingredient in the dosage form and is equivalent to 5%～50% of crude drug summation.
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| CN105363021A (en) * | 2015-11-13 | 2016-03-02 | 邢月军 | Gel for promoting wound healing |
| CN105943691A (en) * | 2016-06-21 | 2016-09-21 | 赵锋 | Traditional Chinese medicine preparation for treating keloid and preparation method thereof |
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Non-Patent Citations (4)
| Title |
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| 急性伤口治疗中中药外用的药理作用分析. 蔡文智.南方护理学报，第11卷第3期. 2004 |
| 急性伤口治疗中中药外用的药理作用分析. 蔡文智.南方护理学报，第11卷第3期. 2004 * |
| 瘢痕的中医药研究现状. 周艳杰.光明中医，第13卷第79期. 1998 |
| 瘢痕的中医药研究现状. 周艳杰.光明中医，第13卷第79期. 1998 * |
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