CN100569097C - Microbe microcapsules and preparation method thereof - Google Patents
Microbe microcapsules and preparation method thereof Download PDFInfo
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- CN100569097C CN100569097C CNB2007100145208A CN200710014520A CN100569097C CN 100569097 C CN100569097 C CN 100569097C CN B2007100145208 A CNB2007100145208 A CN B2007100145208A CN 200710014520 A CN200710014520 A CN 200710014520A CN 100569097 C CN100569097 C CN 100569097C
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- microcapsules
- lactic acid
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- acid bacteria
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of microbe microcapsules and preparation method thereof, aim to provide a kind of microcapsules of protecting the microorganism formulation activity, this capsule comprises that the raw material of following weight portion makes: the xanthan gum solution of the lactic acid bacteria of 2-4 part, 0.2 part maltodextrin, the sodium alginate soln of 1 part of 1%-3%, 1 part of 1%-3%, the silica of 3-4 part, the fine rice bran or the wheat bran of 3-4 part; Its preparation method has bag quilt and dry two steps.The present invention can be used as the usefulness of the feed addictive of pig, chicken.
Description
Technical field
The present invention relates to a kind of feed addictive, microcapsules that microorganism bag is got up specifically the invention still further relates to its preparation method.
Background technology
At present, the appearance of microorganism formulation product has promoted Developing of Animal Industry.But many factors etc. have material impact to the activity of beneficial microbe bacterial classification in production practices, cause microorganism deactivatedly easily, and the microorganism formulation effect is unsatisfactory.Some bacterial classification acid resistances are poor, and (pH is about 2.0) inactivation under the sour environment in pig, chicken stomach causes the beneficial microbe bacterial classification can not field planting in its enteron aisle, thereby can't bring into play its regulatory function.After the other beneficial microbe fermentation, fermentate moisture height is difficult to mixing in feed, and summer the product gas that rises, be difficult to use.The moisture height, product is short storage period, also unfavorable preservation.Some bacterial classification poor heat resistance are subjected to temperatures involved, the bacterial classification inactivation in feed extruding granulation and high temperature drying process.Multiple factors such as fermentate moisture, baking temperature, gastric acidity, extruding granulation and waiting time are restricting the activity of microorganism formulation, so microorganism formulation not effect sometimes in production practices.
Summary of the invention
Purpose of the present invention is to provide a kind of shortcoming that overcomes prior art, the activity of protection microorganism formulation; make beneficial microbe bacterial classification definite value in pig, chicken enteron aisle, improve feed efficiency, promote pig, chicken growth, prevent disease; environment purification, the microbe microcapsules that period of storage is long.
Another object of the present invention provides the preparation method of microbe microcapsules.
The technical solution used in the present invention is for achieving the above object: this capsule comprises that the raw material of following weight portion makes: the lactic acid bacteria of 2-4 part, 0.2 part maltodextrin, the sodium alginate soln of 1 part of 1%-3%, the xanthan gum solution of 1 part of 1%-3%, the fine rice bran or the wheat bran of the silica of 3-4 part, 3-4 part.
The preparation method of mentioned microorganism microcapsules, it comprises the following steps:
A) bag quilt:
Lactic acid bacteria that 2-4 part is fermented and 0.2 part maltodextrin behind the mixing, mix with the sodium alginate soln of 1 part of 1%-3%, the xanthan gum solution of 1 part of 1%-3% in mixer again.
B) drying:
After in mixer, adding the silica of 3-4 part, begin to stir the 1-2 branch and plant, above-mentioned bag is added by good lactic acid bacteria, and continue to stir 5-10 minute, and then the fine rice bran or the wheat bran of adding 3-4 part.Continue again to stir 5-10 minute, last article are emitted, and at room temperature dry promptly.
The invention has the beneficial effects as follows: microorganism formulation can be isolated the moisture in microorganism and the feed, mineral element behind the bag quilt, prolong its storage period, it is put into feed can make microorganism formulation not be destroyed at the gastric juice sour environment, has enteric solubility, thereby improve the quantity that the microorganism lactic acid bacteria plays a role in enteron aisle, promote pig, chicken growth, prevent disease, environment purification; Improve the hear resistance of lactic acid bacteria, can make the influence of its withstand high temperatures in the extruding pelletization.
The specific embodiment
The umber of constitutive material of the present invention and each raw material and preparation method are same as the summary of the invention part.
The test example:
1 materials and methods
1.1 test material, place and cycle
(every gram contains effective viable count 4 * 10 to microbe microcapsules
10Individual): Shandong Zheng-wang Industry And Trade Co., Ltd provides: terramycin: Shandong Zheng-wang Industry And Trade Co., Ltd provides: the microorganism formulation that does not wrap quilt: Shandong Zheng-wang Industry And Trade Co., Ltd provides.
Manual simulation's gastric juice and manual simulation's intestinal juice.
108 of Du of 35 age in days weanling weights, 9.13 ± 0.60kg * big * long tri-crossbreeding, the Wucheng County pig farm provides.
Test site is the pig farm, Wucheng County.
Experimental period is 40 days.
1.2 method
1.2.1 count plate adopts the spread plate counting, cultivates 48-60h in 37 ℃ of anaerobism.
1.2.2 the test group experiment adopts single-factor to design at random, and 108 weanling pigs are close by body weight, the principle of sex ratio basically identical is divided into 3 groups at random to be handled, and each group is handled 3 repetitions, and each repeats 12 pigs.Handle 1 group of basal diet+terramycin 200mg/kg; Handling 2 groups is basal diet+do not wrap by microorganism formulation 200mg/kg; Handling 3 groups is basal diet+microcapsules 200mg/kg.Basal diet is a corn dregs of beans type.
Table 1 basal diet is formed and nutritional labeling
| Raw material | Ratio | Nutritional labeling | Content |
| Corn | 62.25 | Digestible energy (MJ/kg) | 13.42 |
| Dregs of beans | 27.0 | Crude protein (%) | 19.22 |
| Fish meal | 3.0 | Calcium (%) | 0.88 |
| Whey powder | 5.0 | Total phosphorus (%) | 0.7 |
| Salt | 0.3 | Lysine (%) | 1.12 |
| Calcium monohydrogen phosphate | 1.1 | Methionine (%) | 0.69 |
| Stone flour | 1.0 | ||
| Methionine | 0.05 | ||
| Lysine | 0.3 | ||
| Premix | 1.0 |
Annotate: premix is provided by Shandong Zheng-wang Industry And Trade Co., Ltd
1.3 feeding and management:
Test pig is raised in same circle, and free choice feeding is freely drunk water, and carries out thermal insulation.Test is carried out observing the piglet mental status in the process, and record respectively repeats grice diarrhoea number every day respectively, and experimental period finishes that piglet is carried out individuality weighs, and the pig starter feed consumption respectively organized in record.Duration of test carries out health and epidemic prevention and sterilization by pig farm routine immunization operation sequence.
1.4 testing index:
Average daily gain, feed intake, feed conversion rate, diarrhea rate.
1.5 data analysis:
Test data is carried out statistical analysis with SAS software.
2 results and discussion
2.1 the mensuration of microcapsules enteric solubility
The 0.1g microcapsules are placed the triangular flask that fills 100ml manual simulation intestinal juice (pH6.8) and manual simulation's gastric juice (pH2.0) respectively, stir in 37 ± 1 ℃ of waters bath with thermostatic control, mixing speed is no more than 200r/min, carries out count plate respectively at taking out behind 15min, the 60min
[4]The result is as shown in table 2
The disintegration result of table 2 microcapsules in manual simulation's gastric juice and manual simulation's intestinal juice
| 15min | 60min | |
| Manual simulation's gastric juice | 3.0×10 6 | 1.0×10 6 |
| Manual simulation's intestinal juice | 3.6×10 10 | 3.8×10 10 |
With the microcapsules prolongation of time in simulated gastric fluid, clump count changes when 15min and 60min not quite, and the 1h not disintegration substantially in simulated gastric fluid of these microcapsules is described as seen from Table 2.These microcapsules 15min in simulated intestinal fluid just can discharge more than 90% of viable count, and the disintegration in the 15min under the simulated intestinal fluid alkaline environment of these microcapsules is described, disintegrating property is good, and product has enteric solubility.
2.2 microcapsules are to the influence of weanling pig production performance
Table 3 piglet production performance and diarrhoea result
| Handle 1 group | Handle 2 groups | Handle 3 groups | |
| Number (head) | 36 | 36 | 36 |
| Initial weight (kg/ head) | 9.15±0.59 | 9.12±0.61 | 9.14±0.62 |
| End heavy (kg/ head) | 23.95±1.02 | 23.78±1.12 | 25.68±1.22 |
| Net gain (kg/ head) | 14.8±0.87 | 14.66±0.76 | 16.52±0.81 |
| Daily gain (g/ head) | 370 | 367 | 413 |
| Feed consumption (kg/ head) | 33.45 | 32.78 | 35.89 |
| Feedstuff-meat ratio | 2.26±0.15∶1 | 2.24±0.17∶1 | 2.12±0.13∶1 |
| Diarrhea rate (%) | 11.2 | 9.4 | 0 |
Annotate: diarrhea rate (%)=(a diarrhoea number * diarrhoea fate)/(pig number of elements * test fate) * 100%
As can be seen from Table 3, it is 413g that interpolation microcapsules bag is handled 3 groups of average daily gains by microorganism formulation, adds 1 group of the processing of terramycin and add not wrap by 2 groups of average daily gains of the processing of microorganism being respectively 370g and 367g.Handle 3 groups and handle 1 group, handle 2 groups and compare, daily gain has improved 11.6% and 12.5% respectively, and significant difference (P＜0.05) is handled 1 group and handle between 2 groups difference not significantly (P＞0.05).Handle 3 groups feedstuff-meat ratio and compare for 2 groups with handling 1 group and processing, significant difference (P＜0.05), and handle 1 group and to handle between 2 groups difference not remarkable.It is 0 that diarrhea rate is handled 3 groups, does not have diarrhoea, handles 1 group and to handle between 2 groups diarrhea rate difference not remarkable.
In this test, the microbe microcapsules that uses not disintegration substantially under the low pH environment of simulated gastric fluid, 15 minutes is disintegration in simulated intestinal fluid, shows that this product enteric solubility is good, simultaneously, also show and use this production technology can improve the living bacteria count that enteron aisle plays a role, thereby the inhibition growth of harmful intestinal tract bacteria promotes digesting and assimilating of nutriment, improves body immunity, reduce diarrhea rate, thereby further improve production performance.After using this product, indexs such as the daily gain of pig, feedstuff-meat ratio and diarrhea rate all are better than not wrapping the microorganism group and the antibiotic group of quilt.Because this product is harmless, safely, have no side effect.Therefore this product is a kind of novel green additive.
Claims (1)
1, a kind of microbe microcapsules is characterized in that this capsule comprises that the raw material of following weight portion makes: the xanthan gum solution of the lactic acid bacteria of 2-4 part, 0.2 part maltodextrin, the sodium alginate soln of 1 part of 1%-3%, 1 part of 1%-3%, the silica of 3-4 part, the fine rice bran or the wheat bran of 3-4 part;
The preparation method of mentioned microorganism microcapsules, it comprises the following steps:
A) bag quilt:
Lactic acid bacteria that 2-4 part is fermented and 0.2 part maltodextrin behind the mixing, mix with the sodium alginate soln of 1 part of 1%-3% and the xanthan gum solution of 1 part of 1%-3% in mixer again;
B) drying:
After in mixer, adding the silica of 3-4 part, begin to stir the 1-2 branch and plant, above-mentioned bag is added by good lactic acid bacteria, and continue to stir 5-10 minute, and then add the fine rice bran or the wheat bran of 3-4 part, continue again to stir 5-10 minute, last article are emitted, and at room temperature dry promptly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100145208A CN100569097C (en) | 2007-04-09 | 2007-04-09 | Microbe microcapsules and preparation method thereof |
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| CNB2007100145208A CN100569097C (en) | 2007-04-09 | 2007-04-09 | Microbe microcapsules and preparation method thereof |
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| Publication Number | Publication Date |
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| CN101032279A CN101032279A (en) | 2007-09-12 |
| CN100569097C true CN100569097C (en) | 2009-12-16 |
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| CNB2007100145208A Expired - Fee Related CN100569097C (en) | 2007-04-09 | 2007-04-09 | Microbe microcapsules and preparation method thereof |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101642189B (en) * | 2008-08-07 | 2012-05-30 | 北京大北农科技集团股份有限公司 | Microcapsule compound feed additive and preparation method thereof |
| CN101953468B (en) * | 2009-07-13 | 2012-09-05 | 烟台鸿瑞集团生物科技园有限公司 | Collagen peptide-containing lactic acid bacterium powder |
| CN101912046B (en) * | 2010-09-01 | 2013-01-09 | 北京市农林科学院 | Porcine lactobacillus plantarum microcapsules and application thereof |
| CN102352064B (en) * | 2011-07-06 | 2013-05-22 | 丁邦瑞 | Dual-degradant additive for promoting photo oxidative degradation and biodegradation of polymer |
| CN102578415B (en) * | 2012-02-23 | 2016-01-20 | 北京大北农科技集团股份有限公司 | Yue Shi lactobacillus improves the application in bird egg laying performance and Egg Quality feed in preparation |
| CN103275966B (en) * | 2013-06-04 | 2014-10-22 | 国家粮食局科学研究院 | Method for preparing coating microcapsule before microbial fermentation by utilizing common fermentation tank |
-
2007
- 2007-04-09 CN CNB2007100145208A patent/CN100569097C/en not_active Expired - Fee Related
Non-Patent Citations (8)
| Title |
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| 乳酸菌微胶囊化的研究. 李祥明等.饲料研究，第12期. 1998 |
| 乳酸菌微胶囊化的研究. 李祥明等.饲料研究，第12期. 1998 * |
| 微胶囊技术及其在饲料工业中的应用. 周锦兰等.饲料工业，第23卷第6期. 2002 |
| 微胶囊技术及其在饲料工业中的应用. 周锦兰等.饲料工业，第23卷第6期. 2002 * |
| 肠溶性益生菌微胶囊的研究. 阳晖等.食品研究与开发，第27卷第1期. 2006 |
| 肠溶性益生菌微胶囊的研究. 阳晖等.食品研究与开发，第27卷第1期. 2006 * |
| 饲料添加剂微胶囊化方法. 梁兆.中国饲料，第6期. 1998 |
| 饲料添加剂微胶囊化方法. 梁兆.中国饲料，第6期. 1998 * |
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| CN101032279A (en) | 2007-09-12 |
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| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: SHENG QINGKAI Free format text: FORMER OWNER: SHANDONG ZHENG-WANG INDUSTRY AND TRADE CO., LTD. Effective date: 20090619 |
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| C41 | Transfer of patent application or patent right or utility model | ||
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Effective date of registration: 20090619 Address after: Ji'nan City, Shandong Province, mulberry Road No. 10 post encoding: 250100 Applicant after: Sheng Qingkai Address before: Shandong city in Tengzhou Province Economic Development Zone No. 399 spring vine post encoding: 277500 Applicant before: Shandong Zhengwang Pearl-Lustered Pigments Co., Ltd. |
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| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20070912 Assignee: Shandong Huamutianyuan Animal Health-care Stock Co., Ltd. Assignor: Sheng Qingkai Contract record no.: 2010370000048 Denomination of invention: Microbial microcapsule and preparation method thereof Granted publication date: 20091216 License type: Exclusive License Record date: 20100210 |
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| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20091216 Termination date: 20130409 |