CN100560709C - The impact cavity that is used for cell wall breaking - Google Patents
The impact cavity that is used for cell wall breaking Download PDFInfo
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- CN100560709C CN100560709C CN 200610018944 CN200610018944A CN100560709C CN 100560709 C CN100560709 C CN 100560709C CN 200610018944 CN200610018944 CN 200610018944 CN 200610018944 A CN200610018944 A CN 200610018944A CN 100560709 C CN100560709 C CN 100560709C
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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Abstract
The present invention relates to be used for the impact cavity of cell wall breaking, comprise the secondary chamber of interconnected end to end binary master chamber and monobasic, binary master chamber is made up of main pipe and the intravital duct of the person in charge; The secondary chamber of monobasic is made up of looped pipeline body and the intravital duct of looped pipeline; Wherein: be responsible for intravital duct by preceding latter linked main feed-pipe, the first main resonance pipe, the second main resonance pipe, host buffer pipe, main isocon, main impact tube, power stream pipe, cardinal process become pipe successively, chief editor's stream is managed and main discharge nozzle is formed; The intravital duct of looped pipeline is by preceding latter linked secondary feed-pipe, secondary resonatron, secondary buffer tube, secondary isocon, secondary impact tube, secondary jet pipe, secondary expansion stream pipe and secondary discharge nozzle are formed successively.The present invention adopts the combination, particularly binary main resonance pipe in binary master chamber or the secondary chamber of monobasic, tiny bifilar isocon, tiny structures such as secondary jet pipe, allows high velocity liquid flow sharp impacts.The further fragmentation of the cell walls of the material in the suspension can be obtained fine debris.
Description
Technical field
The present invention relates to impact cavity, especially for the impact cavity of cell wall breaking.Be mainly used in fields such as medicine, biology, food, makeup, the cell granulations in the liquid towards object carries out effects such as particle pulverizing, broken wall, refinement, homogenization.
Background technology
To the cell wall breaking of cell granulationses such as spore powder, generally adopt biological enzyme and physical mechanical method.Biological enzyme mainly is to utilize cellulase pair cell wall biodegrade; The physical mechanical method mainly contains modes such as solid phase method, liquid phase method, vapor phase process and ultrasonic wave.Solid phase method mainly is by solid-state grinding broken wall, vapor phase process mainly is by the high velocity air effect of impact, ultrasonic wave is mainly by frequency modulation oscillating action broken wall, and liquid phase method to be the pair cell particle be made into suspension liquid, utilize valve homogenizer and microjet clarifixator to carry out liquid phase and pulverize and reach the broken wall purpose.But the cell wall breaking effect of the material that the impact cavity of common cell wall breaking obtains is undesirable.Be embodied in and have only the part cell, also have a considerable amount of cells not by broken wall by broken wall.
Summary of the invention
The object of the present invention is to provide a kind of impact cavity that is used for cell wall breaking, be used to obtain the high material of cell walls percentage of damage.
Technical scheme of the present invention is:
The impact cavity that is used for cell wall breaking comprises the secondary chamber of interconnected end to end binary master chamber and monobasic, and binary master chamber is made up of main pipe and the intravital duct of the person in charge; The secondary chamber of monobasic is made up of looped pipeline body and the intravital duct of looped pipeline; Wherein:
Be responsible for intravital duct by preceding latter linked main feed-pipe, the first main resonance pipe, the second main resonance pipe, host buffer pipe, main isocon, main impact tube, power stream pipe, cardinal process change pipe, main contracted flow pipe and main discharge nozzle are formed successively;
The diameter of the first main resonance pipe and the second main resonance pipe is greater than the diameter of main feed-pipe and host buffer pipe; Be provided with sprue between the host buffer Guan Yuzhu impact tube, symmetry has tiny barrel and forms main isocon about the sprue; The diameter of power stream pipe becomes the diameter of pipe, main contracted flow pipe and main discharge nozzle less than cardinal process; Cardinal process becomes the diameter of the diameter of pipe greater than main contracted flow pipe; The diameter of main contracted flow pipe is less than the diameter of main discharge nozzle;
The intravital duct of looped pipeline is by preceding latter linked secondary feed-pipe, secondary resonatron, secondary buffer tube, secondary isocon, secondary impact tube, secondary jet pipe, secondary expansion stream pipe and secondary discharge nozzle are formed successively;
Secondary resonatron diameter is greater than the diameter of secondary feed-pipe and secondary buffer tube; Be provided with sprue between secondary buffer tube and the secondary impact tube, symmetry has tiny barrel and forms secondary isocon about the sprue; The diameter of secondary jet pipe expands the diameter of stream pipe and secondary discharge nozzle less than pair; The secondary diameter that expands the diameter of stream pipe less than secondary discharge nozzle.
The impact cavity that is used for cell wall breaking, wherein: the main discharge nozzle in binary master chamber is connected with the secondary feed-pipe in the secondary chamber of monobasic; The main feed-pipe in binary master chamber is in communication with the outside; The secondary discharge nozzle in the secondary chamber of monobasic is in communication with the outside.
The impact cavity that is used for cell wall breaking, wherein: the secondary discharge nozzle in the secondary chamber of monobasic is connected with the main feed-pipe in binary master chamber; The secondary feed-pipe in the secondary chamber of monobasic is in communication with the outside; The main discharge nozzle in binary master chamber is in communication with the outside.
The origin of the secondary chamber of binary master chamber and monobasic title is: binary master chamber comprises the first main resonance pipe, the second main resonance pipe, and the secondary chamber of monobasic includes only a secondary resonatron.
Principle of work:
Elder generation is material suspended in liquor with preliminary fragmentation, relies on to have high-tension apparatus generation high velocity liquid stream now.High velocity liquid stream produces resonance through resonatron, and liquid stream jet velocity further improves, and two strands of high velocity liquid streams are done buffering slightly in buffer tube, and oppressed entering rapidly forms two strands of high velocity liquid streams and sharp impacts in impact tube in the tiny isocon of diameter again.Produce a series of effects such as high speed shear effect, cavitation, vibration and oscillation effect, vortex effect, expanded effect, instantaneous temperature and instantaneous pressure drop in the working process, thus the material particles in the suspension is further broken.
Suspension liquid enters the feed-pipe of the impact cavity that is used for cell wall breaking under the high-pressure pump effect, cylindrical cavity is arranged as resonatron at the impact cavity that is used for cell wall breaking, this helps producing the self-vibration pulsing jet, it is right that fluidic disturbance frequency and chamber natural frequency are complementary, motive fluid resonance produces big amplitude, high-frequency oscillating pressure.After fluid is divided into two strands of high velocity liquid streams, because its powerful longitudinal velocity difference causes huge shearing action.When two bursts of positive collisions of identical high-velocity fluid, fluid loses the speed on the original flow direction suddenly, produces huge Impact energy.Bump against strongly between the solid particulate in the fluid.Simultaneously, fall, can form the effect of intensive vortex at impingement region because the huge pressure of generation is bumped against in the flow field.The bump and the friction of strong horizontal direction appears in fluid, and the particle in the fluid is laterally bump and friction of experience again behind vertical bump.Along with clarifixator ram pump output rating increases, fluid obtains big more momentum and clashes into.Except that the effect of vertical direction and horizontal direction, the vortex effect of other all directions all has different bump of degree and friction.Fluid bumps against the back huge pressure of generation and falls, and occurs cavitation when pressure is lower than the vapour pressure of liquid.When the increase along with the time and intensity of cavitation, the power that cavitation erosion produces also is indubitable, and its huge energy is then more to be married again around in the flow field, thereby stream field produces the destruction bigger than flow field in the valve homogenizer spool.
For the impact cavity that is used for cell wall breaking, it is mainly combined by binary master chamber and the secondary chamber of monobasic.Use the shell-broken effect in the secondary chamber of binary master chamber or monobasic not as binary master chamber and the secondary chamber of monobasic effect of Combination separately.
The invention has the advantages that: adopt the combination, particularly binary main resonance pipe in the secondary chamber of binary master chamber or monobasic, tiny bifilar isocon, tiny structures such as secondary jet pipe, allow high velocity liquid flow sharp impacts.Produce a series of effects such as high speed shear effect, cavitation, vibration and oscillation effect, vortex effect, expanded effect, instantaneous temperature and instantaneous pressure drop in the working process, thereby the further fragmentation of the cell walls of the material in the suspension is obtained fine debris.More complete cell wall breaking is very difficult, and adopting binary main resonance pipe is for the resonance through twice obtains more high speed liquid stream, helps more complete cell wall breaking after more high speed liquid stream brute force clashes.
For example for Ganoderma spore powder, under same processing condition: use binary master chamber separately, use the shell-broken effect in the secondary chamber of monobasic to be respectively 70.2%, 75.2% separately.The shell-broken effect of the two-chamber combination that the secondary feed-pipe in the main discharge nozzle in binary master chamber and the secondary chamber of monobasic is connected can reach 94.6%, and the shell-broken effect of the two-chamber combination that the secondary discharge nozzle in the secondary chamber of monobasic and the main feed-pipe in binary master chamber are connected can reach 96.2%.
Description of drawings
The structural representation that the two-chamber that Fig. 1 is connected for the secondary feed-pipe 1b in the main discharge nozzle 9a in binary master of the present invention chamber and the secondary chamber of monobasic makes up;
The structural representation that the two-chamber that Fig. 2 is connected for the main feed-pipe 1a in the secondary discharge nozzle 9b in the secondary chamber of monobasic of the present invention and binary master chamber makes up.
Fig. 3: undressed Black Ganoderma spore powder pattern
Fig. 4: through the Black Ganoderma spore powder pattern of the secondary chamber of monobasic individual curing 1 time
Fig. 5: handle 2 times Black Ganoderma spore powder pattern through binary master chamber
Fig. 6: through the secondary chamber of monobasic in preceding, binary master chamber at the Black Ganoderma spore powder pattern of aftertreatment 2 times
Fig. 7: handle 3 times Black Ganoderma spore powder pattern through the valve formula high pressure homogenizer of routine
Reference numeral: main feed-pipe 1a, the first main resonance pipe 2a1, the second main resonance pipe 2a2, host buffer pipe 3a, main isocon 4a, main impact tube 5a, power stream pipe 6a, cardinal process become pipe 7a, main contracted flow pipe 8a, main discharge nozzle 9a, secondary feed-pipe 1b, secondary resonatron 2b, secondary buffer tube 3b, secondary isocon 4b, secondary impact tube 5b, secondary jet pipe 6b, secondary stream pipe 10b, the secondary discharge nozzle 9b of expanding.
Embodiment
Embodiment 1
The impact cavity that is used for cell wall breaking comprises the secondary chamber of interconnected end to end binary master chamber and monobasic, and binary master chamber is made up of main pipe and the intravital duct of the person in charge; The secondary chamber of monobasic is made up of looped pipeline body and the intravital duct of looped pipeline; Wherein:
Be responsible for intravital duct by preceding latter linked main feed-pipe 1a, the first main resonance pipe 2a1, the second main resonance pipe 2a2, host buffer pipe 3a, main isocon 4a, main impact tube 5a, power stream pipe 6a, cardinal process change pipe 7a, main contracted flow pipe 8a and main discharge nozzle 9a form successively;
The diameter of the first main resonance pipe 2a1 and the second main resonance pipe 2a2 is greater than the diameter of main feed-pipe 1a and host buffer pipe 3a; Be provided with sprue between host buffer pipe 3a and the main impact tube 5a, symmetry has tiny barrel and forms main isocon 4a about the sprue; The diameter of power stream pipe 6a becomes the diameter of pipe 7a, main contracted flow pipe 8a and main discharge nozzle 9a less than cardinal process; Cardinal process becomes the diameter of the diameter of pipe 7a greater than main contracted flow pipe 8a; The diameter of main contracted flow pipe 8a is less than the diameter of main discharge nozzle 9a;
The intravital duct of looped pipeline is by preceding latter linked secondary feed-pipe 1b, secondary resonatron 2b, secondary buffer tube 3b, secondary isocon 4b, secondary impact tube 5b, secondary jet pipe 6b, secondary expansion stream pipe 10b and secondary discharge nozzle 9b form successively;
Embodiment 2
The impact cavity that is used for cell wall breaking, wherein: the main discharge nozzle 9a in binary master chamber is connected with the secondary feed-pipe 1b in the secondary chamber of monobasic; The main feed-pipe 1a in binary master chamber is in communication with the outside; The secondary discharge nozzle 9b in the secondary chamber of monobasic is in communication with the outside.All the other are with embodiment 1.
Embodiment 3
The impact cavity that is used for cell wall breaking, wherein: the secondary discharge nozzle 9b in the secondary chamber of monobasic is connected with the main feed-pipe 1a in binary master chamber; The secondary feed-pipe 1b in the secondary chamber of monobasic is in communication with the outside; The main discharge nozzle 9a in binary master chamber is in communication with the outside.All the other are with embodiment 1.
Embodiment 4
Broken wall with the Black Ganoderma spore powder is further described below:
With the Black Ganoderma spore powder is raw material, disposes 10% Black Ganoderma spore powder suspension liquid earlier, and the Microfluidizer M-700 that produces with U.S. Microfluidic company is that main equipment provides propulsion source, uses secondary chamber respectively separately; Main chamber; Secondary chamber preceding main chamber after mode, handle 10% Black Ganoderma spore powder suspension liquid.To stir with Black Ganoderma spore powder sample before and after the certain density processing, pipette 20ul on slide glass with liquid-transfering gun, and dropping 10ul Switzerland dye liquor, make smear, under XS-402 type fluorescence trinocular microscope, amplify 400 times, to its granular size, pattern, the features such as adsorptivity of dyestuff are measured and observed, result such as Fig. 4, Fig. 5, shown in Figure 6.
If adopt secondary chamber preceding main chamber after the secondary feed-pipe 1b in mode secondary chamber of monobasic when handling be connected with the high-pressure section of Microfluidizer M-700, Black Ganoderma spore powder suspension liquid is finished broken wall in secondary chamber of monobasic and binary master chamber after, the main discharge nozzle 9a through binary master chamber discharges again.
When the mode in the secondary chamber of use is handled separately; The secondary feed-pipe 1b in the secondary chamber of monobasic is connected with the high-pressure section of MicrofluidizcrM-700, and Black Ganoderma spore powder suspension liquid is finished broken wall in the secondary chamber of monobasic after, the secondary discharge nozzle 9b through the secondary chamber of monobasic discharges again.
When the mode in the main chamber of use is handled separately; The main feed-pipe 1a in binary master chamber is connected with the high-pressure section of MicrofluidizcrM-700, and Black Ganoderma spore powder suspension liquid is finished broken wall in binary master chamber after, the main discharge nozzle 9a through binary master chamber discharges again.
All the other are with embodiment 3.
Embodiment 5
Broken wall with the Black Ganoderma spore powder is further described below:
With the Black Ganoderma spore powder is raw material, the Microfluidizer M-700 that produces with U.S. Microfluidic company is a main equipment, be used for the fluid impact cavity major-minor chamber series connection of cell wall breaking, adopt main chamber preceding secondary chamber after mode, handle 10% Black Ganoderma spore powder suspension liquid.Result and shown in Figure 6 similar.
Adopt main chamber preceding secondary chamber after the main feed-pipe 1a in mode binary master chamber when handling be connected with the high-pressure section of Microfluidizer M-700, Black Ganoderma spore powder suspension liquid is finished broken wall in binary master chamber and the secondary chamber of monobasic after, the secondary discharge nozzle 9b through the secondary chamber of monobasic discharges again.
All the other are with embodiment 2 and embodiment 4.
Embodiment 6
Broken wall with 10% Black Ganoderma spore powder suspension liquid is further described below: adopt traditional valve formula high pressure homogenizer shell-broken effect such as Fig. 7.
Above experimental result shows that traditional valve formula high pressure homogenizer shell-broken effect is undesirable.For the impact cavity that is used for cell wall breaking, it is mainly combined by binary master chamber and the secondary chamber of monobasic.Use the shell-broken effect in the secondary chamber of binary master chamber or monobasic not as binary master chamber and the secondary chamber of monobasic effect of Combination separately.
Claims (2)
1, the impact cavity that is used for cell wall breaking, binary master chamber is made up of main pipe and the intravital duct of the person in charge; The secondary chamber of monobasic is made up of looped pipeline body and the intravital duct of looped pipeline; It is characterized in that:
Be responsible for intravital duct by preceding latter linked main feed-pipe (1a), the first main resonance pipe (2a1), the second main resonance pipe (2a2), host buffer pipe (3a), main isocon (4a), main impact tube (5a), power stream pipe (6a), cardinal process change pipe (7a), main contracted flow pipe (8a) and main discharge nozzle (9a) are formed successively;
The diameter of the first main resonance pipe (2a1) and the second main resonance pipe (2a2) is greater than the diameter of main feed-pipe (1a) and host buffer pipe (3a); Be provided with sprue between host buffer pipe (3a) and the main impact tube (5a), symmetry has tiny barrel and forms main isocon (4a) about the sprue; The diameter of power stream pipe (6a) becomes the diameter of pipe (7a), main contracted flow pipe (8a) and main discharge nozzle (9a) less than cardinal process; Cardinal process becomes the diameter of the diameter of pipe (7a) greater than main contracted flow pipe (8a); The diameter of main contracted flow pipe (8a) is less than the diameter of main discharge nozzle (9a);
The intravital duct of looped pipeline is by preceding latter linked secondary feed-pipe (1b), secondary resonatron (2b), secondary buffer tube (3b), secondary isocon (4b), secondary impact tube (5b), secondary jet pipe (6b), secondary expansion stream pipe (10b) and secondary discharge nozzle (9b) are formed successively;
Secondary resonatron (2b) diameter is greater than the diameter of secondary feed-pipe (1b) and secondary buffer tube (3b); Be provided with sprue between secondary buffer tube (3b) and the secondary impact tube (5b), symmetry has tiny barrel and forms secondary isocon (4b) about the sprue; The diameter of secondary jet pipe (6b) expands the diameter of stream pipe (10b) and secondary discharge nozzle (9b) less than pair; The secondary diameter that expands the diameter of stream pipe (10b) less than secondary discharge nozzle (9b);
The main discharge nozzle (9a) in binary master chamber is connected with the secondary feed-pipe (1b) in the secondary chamber of monobasic; The main feed-pipe (1a) in binary master chamber is in communication with the outside; The secondary discharge nozzle (9b) in the secondary chamber of monobasic is in communication with the outside.
2, the impact cavity that is used for cell wall breaking, binary master chamber is made up of main pipe and the intravital duct of the person in charge; The secondary chamber of monobasic is made up of looped pipeline body and the intravital duct of looped pipeline; It is characterized in that:
Be responsible for intravital duct by preceding latter linked main feed-pipe (1a), the first main resonance pipe (2a1), the second main resonance pipe (2a2), host buffer pipe (3a), main isocon (4a), main impact tube (5a), power stream pipe (6a), cardinal process change pipe (7a), main contracted flow pipe (8a) and main discharge nozzle (9a) are formed successively;
The diameter of the first main resonance pipe (2a1) and the second main resonance pipe (2a2) is greater than the diameter of main feed-pipe (1a) and host buffer pipe (3a); Be provided with sprue between host buffer pipe (3a) and the main impact tube (5a), symmetry has tiny barrel and forms main isocon (4a) about the sprue; The diameter of power stream pipe (6a) becomes the diameter of pipe (7a), main contracted flow pipe (8a) and main discharge nozzle (9a) less than cardinal process; Cardinal process becomes the diameter of the diameter of pipe (7a) greater than main contracted flow pipe (8a); The diameter of main contracted flow pipe (8a) is less than the diameter of main discharge nozzle (9a);
The intravital duct of looped pipeline is by preceding latter linked secondary feed-pipe (1b), secondary resonatron (2b), secondary buffer tube (3b), secondary isocon (4b), secondary impact tube (5b), secondary jet pipe (6b), secondary expansion stream pipe (10b) and secondary discharge nozzle (9b) are formed successively;
Secondary resonatron (2b) diameter is greater than the diameter of secondary feed-pipe (1b) and secondary buffer tube (3b); Be provided with sprue between secondary buffer tube (3b) and the secondary impact tube (5b), symmetry has tiny barrel and forms secondary isocon (4b) about the sprue; The diameter of secondary jet pipe (6b) expands the diameter of stream pipe (10b) and secondary discharge nozzle (9b) less than pair; The secondary diameter that expands the diameter of stream pipe (10b) less than secondary discharge nozzle (9b);
The secondary discharge nozzle (9b) in the secondary chamber of monobasic is connected with the main feed-pipe (1a) in binary master chamber; The secondary feed-pipe (1b) in the secondary chamber of monobasic is in communication with the outside; The main discharge nozzle (9a) in binary master chamber is in communication with the outside.
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| CN 200610018944 CN100560709C (en) | 2006-04-24 | 2006-04-24 | The impact cavity that is used for cell wall breaking |
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| CN 200610018944 CN100560709C (en) | 2006-04-24 | 2006-04-24 | The impact cavity that is used for cell wall breaking |
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| CN100560709C true CN100560709C (en) | 2009-11-18 |
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| CN102793728A (en) * | 2012-08-24 | 2012-11-28 | 安徽哈博药业有限公司 | High-frequency oscillation wall-broken ganoderma lucidum spore powder |
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Non-Patent Citations (4)
| Title |
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| 微射流均质机的流体动力学行为分析. 刘成梅等.食品科学，第25卷第4期. 2004 |
| 微射流均质机的流体动力学行为分析. 刘成梅等.食品科学，第25卷第4期. 2004 * |
| 脉冲电磁场杀菌的作用机理及其应用. 刘伟等.粮食与食品工业，第12卷第1期. 2005 |
| 脉冲电磁场杀菌的作用机理及其应用. 刘伟等.粮食与食品工业，第12卷第1期. 2005 * |
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