CN100540649C - A kind of Chinese caterpillar fungus - Google Patents
A kind of Chinese caterpillar fungus Download PDFInfo
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- CN100540649C CN100540649C CNB2007101575576A CN200710157557A CN100540649C CN 100540649 C CN100540649 C CN 100540649C CN B2007101575576 A CNB2007101575576 A CN B2007101575576A CN 200710157557 A CN200710157557 A CN 200710157557A CN 100540649 C CN100540649 C CN 100540649C
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Abstract
The invention discloses a kind of white Chinese caterpillar fungus, called after Cordyceps militaris (L.) Link. (Cordyceps militaris (L.:Fr.) Link.), deposit number CGMCC NO.1854, its body colour is a white, have outstanding resistance of oxidation, especially be fit to the Application and Development of cosmetic field; Also can be used on the exploitation of other field, have economic worth.
Description
Technical field
The present invention relates to a kind of Cordyceps militaris (L.) Link., more particularly, relate to the Cordyceps militaris (L.) Link. that a kind of domestication cultivates.
Background technology
Chinese caterpillar fungus is a kind of famous and precious wild Chinese medicinal materials of China, has tonifying deficiency, beneficial vital essence, protects effects such as lung, kidney-nourishing, hemostasis and phlegm, strengthening by means of tonics.Classify China's three big tonics as with genseng, pilose antler.The record of " Cordyceps sinensis Gan Pingbao lung, kidney-nourishing are mended marrow, hemostasis and phlegm, phthisical cough, it is all good to control diaphragm disease " was just arranged in " new compilation of materia medica " as a Seventh Five-Year Plan in 7 years.The traditional Chinese medical science thinks that Chinese caterpillar fungus goes into lung kidney two warps, can tonifying lung the moon, again can kidney-replenishing, and cure mainly and suffer from a deficiency of the kidney, impotence and seminal emission, soreness of waist and knee joint, weak after being ill, the chronic cough weakness, phthisical cough phlegm blood, spontaneous sweatings etc. are unique a kind of balances simultaneously, regulate the Chinese medicine of negative and positive.Kind surplus the known Chinese caterpillar fungus class in the whole world has 350, China has 62 kinds at present, but the most expensive has only Cordyceps and Cordyceps militaris.Archaism is said: rather want one of Chinese caterpillar fungus, not the full car of gold and jade; Among the peoplely then be referred to as " gold of living ".
Cordyceps militaris (L.) Link. (having another name called Cordyceps militaris, Cordyceps militaris (L.) Link.) (C.militaris (L.) Link.), Cordyceps militaris (L.) Link. is an Ascomycotina, the ergot Zoopagales, the type species of Clavicipitaceae, Cordyceps, formal name used at school are Cordycepsmilitaris, and worldwide distribution natural resource quantity seldom.By stroma (being careless part) and sclerotium (being the corpse part of worm) complex body dimerous.Winter, larva lived in seclusion in the soil, and the mushroom parasitism is wherein drawn nutrition, was full of mycelia in the larva body and dead.To summer, on the larva corpse, bear seedling, the likeness in form grass, front and back were gathered and were got the Summer Solstice.Domesticly found first in Jilin Province, be tested and appraised, think that it and Cordyceps sinensis (Cordyceps Sinensis (Berk.) Sacc.) are same genus, name and be pupa grass (C.militaris (L.) Link) in 1958.Main product is (Kunming, peacefulness, river and mountain), Jilin (Antu, Yongji), Liaoning (Shenyang), the Inner Mongol (Zhelimu League) in Yunnan, is born on the pupal cell of lepidopterous insects in pin, broad-leaf forest or the mixed forest surface soil layer.Can adopt that silkworm and cocoon chrysalis are artificial to be cultivated in batches, drug effect, pharmacology are similar to wild species even better.Cordyceps militaris has several unrivaled advantages than Cordyceps sinensis: the one, and Cordyceps militaris is the type species of Cordyceps, and is widely distributed, by the countries in the world scholar is familiar with and accepts.The 2nd, Cordyceps militaris has been bred complete stroma under artificial condition.The 3rd, Cordyceps militaris contain cordycepin (have another name called cordycepin Cordycepin, promptly 3 '-Dexyadenosine C10H13O3N5) and Cordyceps polysaccharide, its unique pharmacological action causes the great attention of pharmacy circle day by day.Because Cordyceps militaris (L.) Link. has above advantage, becomes the outstanding person in the medicinal Cordyceps in the Cordyceps.Because the wild Chinese caterpillar fungus scarcity of resources costs an arm and a leg, and is difficult to popularize.
At present, succeed, but do not see the report of candida species as yet with biotechnology method artificial culture Cordyceps militaris (L.) Link..
Summary of the invention
The invention provides the new bacterial strain of a kind of Cordyceps militaris (L.) Link., body colour is a white, and using value has potentiality.
A kind of Cordyceps militaris (L.) Link. provided by the invention, Latin formal name used at school Cordyceps militaris (L.:Fr.) Link is at China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, deposit number CGMCCNO.1854; The microorganism (strain) of ginseng certificate is PA-H 4. (a bacterial strain name).
Wherein, Cordyceps militaris (L.) Link. integral body of the present invention is white; Stroma or sporophore column, single or grow thickly, or branch is arranged, the top that has is expanded and is bar-shaped, long 7.5~10 centimetres, thick 0.3~0.5 centimetre, the fragrance flavor is arranged.And its cordycepin content is 0.8 * 10
4~1.2 * 10
4Milligram, the cordycepin average content is 1.1 * 10
4Milligram.In addition, other main content such as following tables:
Interventions Requested | Unit | Experimental result |
Cordycepic acid | % | 10~16 |
The SOD enzyme | μ/g | 170~387 |
Polysaccharide | % | 12~16 |
Protein | % | 25~41 |
White Cordyceps militaris (L.) Link. provided by the invention has using value, especially is fit to the Application and Development of cosmetic field.Reason is that traditional Cordyceps militaris (L.) Link. color is yellow, because the existence of yellow composition needs to adopt the expensive means of discoloring, this has limited Cordyceps militaris (L.) Link. and has been used for cosmetic development on cost.For this reason, through the domestication, turn out white Chinese caterpillar fungus of the present invention, after testing, the resistance of oxidation of this white Cordyceps militaris (L.) Link. is better than common Chinese caterpillar fungus far away, particularly is fit to the Application and Development of cosmetic field.And white Cordyceps militaris (L.) Link. of the present invention can be used in other field equally, so its economic worth is difficult to estimate.
Description of drawings
Fig. 1 is the photo of Cordyceps militaris (L.) Link. of the present invention.
The preservation information of the biological material specimens that the application relates to: the ginseng certificate microorganism (strain) for PA-H 4., classification called after Cordyceps militaris (L.) Link. (Cordyceps militaris), in on October 31st, 2006 by China Committee for Culture Collection of Microorganisms common micro-organisms center (be called for short CGMCC) preservation, deposit number CGMCC NO.1854.The CGMCC address is a No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City Institute of Microorganism, Academia Sinica.
Embodiment
As shown in Figure 1, a kind of Chinese caterpillar fungus provided by the invention, called after Cordyceps militaris (L.) Link. (Cordyceps militaris (L.:Fr.) Link.), deposit number CGMCC NO.1854, body colour is a white.
Mention in the detection probation report that Institute of Microorganism, Academia Sinica provides: PA-H 4. for Cordyceps militaris (L.) Link. of the present invention (Cordyceps militaris), " stroma or sporophore column; single or grow thickly; or branch is arranged; the top that has is expanded and is bar-shaped, and long 7.5~10 centimetres, thick 0.3~0.5 centimetre; the fragrance flavor is arranged, and other form tissues are identical with the Cordyceps militaris (L.) Link. of common artificial culture ".Through identifying, " be the Cordyceps militaris (L.) Link. that the domestication cultivates, by its fungi morphological classification; belong to Ascomycotina, ergot Zoopagales Clavicipitales, Clavicipitaceae Clavicipitaceae; Chinese caterpillar fungus Pseudomonas Cordyceps, the Chinese Cordyceps militaris (L.) Link. is called pupa grass, Cordyceps militaris, Cordyceps militaris (L.) Link..Latin formal name used at school Cordyceps militaris (L.:Fr.) Link..This sample belongs to the Cordyceps militaris (L.) Link. white bacterial strain that forms through natural seed selection for many years, has cultivated production, and proterties is stable, has not yet to see the report of candida species, is considered as treasure.Cordyceps militaris (L.) Link. belongs to China's tradition medicinal fungi, and candida species may have more development and application values.Identify that sample retention is at Institute of Microorganism, Academia Sinica fungus sample shop (HMAS).”
In addition, in every kilogram of Cordyceps militaris (L.) Link. of the present invention cordycepin content 0.8 * 10
4~1.2 * 10
4Milligram, average content is 1.1 * 10
4Milligram.Other main content ranges see table:
Interventions Requested | Unit | Experimental result |
Cordycepic acid | % | 10~16 |
The SOD enzyme | μ/g | 170~387 |
Polysaccharide | % | 12~16 |
Protein | % | 25~41 |
White Chinese caterpillar fungus of the present invention after testing has outstanding resistance of oxidation, is fit to very much the Application and Development of cosmetic field.
Its technological process of production of the present invention's white Cordyceps militaris (L.) Link. is similar to common Chinese caterpillar fungus, comprises seed collecting, separates master clock, selects breeding, original seed liquid culture, bottling, sterilization, cultivation, inoculation, the differentiation of annesl original hase, sporophore growth period management, gathers ... step.
Through the concrete artificial culture of implementing, obtained white Cordyceps militaris (L.) Link. of the present invention, detect wherein once cultivating successful Chinese caterpillar fungus, the mean value of its concrete trace element is as follows:
Interventions Requested | Unit | Experimental result |
Aspartic acid | mg/100g | 2534.65 |
The Sue propylhomoserin | mg/100g | 1603.39 |
The Silk propylhomoserin | mg/100g | 1341.80 |
The Grains propylhomoserin | mg/100g | 2736.10 |
Glycine | mg/100g | 1377.71 |
L-Ala | mg/100g | 2227.44 |
Gelucystine | mg/100g | 1922.59 |
Xie Ansuan | mg/100g | 2946.23 |
Methionine(Met) | mg/100g | 629.69 |
Isoleucine | mg/100g | 837.00 |
Leucine | mg/100g | 1251.45 |
Tyrosine | mg/100g | 1945.66 |
Phenylalanine | mg/100g | 540.34 |
Methionin | mg/100g | 1739.91 |
Ammonia | mg/100g | 1388.21 |
Histidine | mg/100g | 1641.92 |
Arginine | mg/100g | 1770.57 |
Proline(Pro) | mg/100g | 2079.92 |
Total amino acid content | mg/100g | 30514.59 |
Cordycepin | mg/kg | 1.1×104 |
Cordycepic acid | % | 13.47 |
The SOD enzyme | μ/g | 386.36 |
Polysaccharide | % | 15.4 |
Protein | % | 37.42 |
Copper | mg/kg | 31.3 |
Calcium | % | 0.037 |
Zinc | mg/kg | 108 |
Selenium | mg/kg | 0.28 |
Vitamins B | mg/kg | 1509.31 |
Vitamin-E | mg/kg | 2.72 |
Vitamin A | mg/kg | 3.68 |
Claims (1)
1, a kind of Chinese caterpillar fungus is characterized in that, name Cordyceps militaris (L.) Link. (Cordyceps militaris), deposit number CGMCC NO.1854.
Priority Applications (1)
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CNB2007101575576A CN100540649C (en) | 2007-10-16 | 2007-10-16 | A kind of Chinese caterpillar fungus |
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CNB2007101575576A CN100540649C (en) | 2007-10-16 | 2007-10-16 | A kind of Chinese caterpillar fungus |
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CN100540649C true CN100540649C (en) | 2009-09-16 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103283479B (en) * | 2012-02-24 | 2014-10-15 | 北京市弘科农业科技服务站 | Cordyceps militaris strain and application thereof as well as method for producing cordyceps militaris |
CN109294922B (en) * | 2018-08-02 | 2021-09-24 | 河北晶懋生物医药科技有限公司 | Preparation method of cordyceps militaris strain and cordyceps militaris strain prepared by same |
CN115491313B (en) * | 2022-10-09 | 2024-01-30 | 辽宁省农业科学院 | Cordyceps militaris white strain and application thereof |
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Non-Patent Citations (2)
Title |
---|
蛹虫草子囊孢子萌发及其后代群体培养性状观察. 梁月等.菌物学报,第24卷第4期. 2005 |
蛹虫草子囊孢子萌发及其后代群体培养性状观察. 梁月等.菌物学报,第24卷第4期. 2005 * |
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