CN100520355C - Sensor array type cold light instrument - Google Patents
Sensor array type cold light instrument Download PDFInfo
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- CN100520355C CN100520355C CNB2004101029164A CN200410102916A CN100520355C CN 100520355 C CN100520355 C CN 100520355C CN B2004101029164 A CNB2004101029164 A CN B2004101029164A CN 200410102916 A CN200410102916 A CN 200410102916A CN 100520355 C CN100520355 C CN 100520355C
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- cold light
- sensor array
- array type
- signal
- light instrument
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Abstract
The invention discloses a luminescence meter which has a detection array, can detect optical signals of plural samples at the same time and receive these optical signals at the same time by photosensor. The invention at least comprises a detection array, which comprises plural detection ends and is used to pick up the luminescence signals emitted by the to-be-detected samples; plural optical signal transmission components, extending to the detection ends, respectively, so as to transmit these luminescence signals; and a photosensor used to receive the luminescence signals transmitted by the optical signal transmission components and convert them into the data able to identify the luminescence signals of the to-be-detected samples.
Description
Technical field
The present invention relates to a kind of cold light arrangement for detecting, especially about a kind of sensor array type cold light instrument (Luminometer), this kind cold light instrument can be detected the light signal of a plurality of samples simultaneously, and can significantly save the detecting time.
Background technology
In the Measurement for Biotechnique, utilize the test pointer of cold light light reaction result, become method of testings commonly used such as the performance of test gene, genetic analysis, immunoassay and cell proliferation in the present laboratory as quantitative or qualitative test.The carrying out of cold light reaction also need add excitation source like that in addition not as the fluorescent reaction, also do not have the danger of radioactive ray element, and the process of sample concentration or dilution also must be arranged unlike traditional ferment immune response simultaneously, and its superiority is easily arranged experimentally.
Known cold light instrument mainly is divided into two classes, and a kind of is the tubular type cold light instrument of the single test tube of detecting, and another kind then is to survey the disc type cold light instrument of reading trace dish (microplate).The tubular type cold light instrument is generally used for sample size test more after a little while because once can only detect a test tube.If desire to carry out the screening analysis of sample size when big, then need utilize the disc type cold light instrument, just can meet the test demand of fairly large amount.With known disc type cold light instrument is example, as shown in Figure 1, this cold light instrument comprises a detecting part 10, these detecting part 10 belows are provided with a sense terminal 11 towards micropore Pan20Chu, this sense terminal 11 is movable to Kong Jing (well) top of carrying sample to be tested when detecting, corresponding with each tested hole well 21, carry out the cold light 15 that cold light reaction is sent in the hole well 21, promptly enter in the middle of the sense terminal 11 by the light entrance 12 of sense terminal 11 front ends.For being assembled, concentrates cold light, this sense terminal 11 is in light entrance 12 tops, be provided with a condenser lens 13 simultaneously, after cold light 15 is assembled through condenser lens 13, be incident upon on the prism 14, (photomultiplier tube is PMT) in 16 to import photomultiplier behind light refraction into, this photomultiplier 16 can change the light signal into electric signal, with further interpretation.The known cold light instrument of this kind only is provided with single sense terminal, therefore when detecting trace dish, this sense terminal must hole well intercropping X, Y-axis to move in proper order, respectively each Kong Jing is detected.This kind detecting mode, on the one hand quite consuming time, the also quite inconvenience of analyzing for extensive screening of test, and these can carry out X, Y-axis is higher to the assembly apparatus price that moves, and raising is provided with the cost of this type cold light instrument.On the other hand, excessive to the mistiming between detecting for the comparatively of short duration test of cold light reaction time if the back takes place in reaction, its result error that time conditions differs that probably also reacts.Very person is still arranged, and what sense terminal was frequent moves around, and after the prolonged use, assembly itself will produce error because of thermal expansion makes the location of sense terminal, and then detecting data is departed from, thereby reduce the accuracy of this instrument.
Summary of the invention
For shortening the detecting time of cold light instrument, keep akin detecting condition, and increase its detecting accuracy, purpose of the present invention is for providing a kind of sensor array type cold light instrument, this cold light instrument comprises one and has the detecting array of a plurality of sense terminals, it can detect the cold light signal of a plurality of samples simultaneously, therefore can increase the detecting speed and the accuracy thereof of sample.
Cold light instrument of the present invention comprises a detecting array, and this detecting array has a plurality of sense terminals, and it is to be used to capture the cold light signal that sample to be tested sends; A plurality of smooth signal transmission assemblies, it is to extend respectively from each sense terminal, uses the transmission cold light signal; And an optical sensing subassembly, this optical sensing subassembly is in order to receiving a plurality of cold light signals that these a plurality of smooth signal transmission assemblies are transmitted, and these signals can be presented via the numerical value or the image mode of electric signal.
When utilizing cold light instrument of the present invention to detect sample, be that respectively this sense terminal that will detect array corresponds respectively to a sample to be tested, the cold light signal that each sample to be tested sent can throw and enter respectively in this sense terminal, and transfer on the optical sensing subassembly by light signal transmission assembly, after this optical sensing subassembly receives these a plurality of cold light signals, those signals can be presented with numerical value or image mode, with the cold light numerical value of further those samples to be tested of interpretation.
The arrangement mode of aforementioned sense terminal, there is no particular restriction, but for detecting moving direction by using can be only carried out to single axial (X axis or Y-axis to), the spacing of each sense terminal and number should conform to the specification single-row or single hurdle of use trace dish.On the other hand, the trace dish specification that cold light instrument of the present invention was suitable for is also unrestricted, and 96 general common holes or 384 holes trace dish are all applicable.If the test tube test then can be provided with according to its number and its spacing adjustment.
Owing to when carrying out the detecting of sample, be mode to become to be listed as, a plurality of samples are detected simultaneously, therefore can significantly reduce the time of detection, so minimizing detecting error also can be shortened in the back that reacts between each sample as far as possible to the mistiming that detecting is produced.Moreover, when moving, only being moving of single axial owing to sense terminal, can significantly reduce because of moving the positioning error of the thermal expansion that is produced repeatedly, for keeping of accuracy sizable help is arranged also.Simultaneously, obtain more or less freelyly owing to only make assembly that single axial moves, and its price is also lower, therefore can reduce the cost of cold light instrument.
Smooth signal transmission assembly used in the present invention, the preferably is an optical fiber, because of it has the little advantage of signal loss.Because optical fiber has the characteristic of total internal reflection, the cold light signal that each sense terminal received can all be transmitted through each optical fiber, and because of the restriction of its angle of light, and the cold light interference of other adjacent holes well that need not to worry.Simultaneously, because optical fiber is not limit by the light refraction angle when transmitting light, and it can extend or tortuous according to apparatus structure, and it is comparatively convenient therefore to design for the configuration of instrument inner assembly.
Optical sensing subassembly used in the present invention, the preferably is for once receiving and can distinguish the optical sensing subassembly of a plurality of smooth signals simultaneously, for example: Charged Coupled Device (charge-coupled device, CCD), complementary matal-oxide semiconductor (complementary metal-oxide semiconductor, CMOS), avalanche diode (avalanche photodiode) or other similar optical sensing subassembly.Above-mentioned optical sensing subassembly all can convert the light signal to image signal, is example with CCD, and it can be accepted and the light signal is recorded as image, as long as after each image signal is converted to data, can detect the cold light numerical value of each sample.As in known technology as the photomultiplier of optical sensing subassembly therefore and be not suitable for the present invention because it can't receive and distinguish a plurality of smooth signals simultaneously, once can only detect a sample.
Below conjunction with figs. is further specified embodiments of the present invention; following cited embodiment is in order to illustrate the present invention; be not in order to limit scope of the present invention; any personage who is familiar with this technology; without departing from the spirit and scope of the present invention; change and retouching when doing a little, so protection scope of the present invention is when looking accompanying being as the criterion that claim defines.
Description of drawings
Synoptic diagram when Fig. 1 carries out the cold light detecting for known invention;
Synoptic diagram when Fig. 2 carries out the cold light detecting for the present invention;
Fig. 3 receives the synoptic diagram of light signal for CCD array of the present invention;
Synoptic diagram when Fig. 4 detects 96 holes trace dish for the present invention.
Among the figure
10 detecting parts
11 sense terminals, 12 light entrances
13 condenser lenses, 14 prisms
15 cold light, 16 photomultipliers
Detecting chambers 17
20 trace dishes, 21 hole wells
30 detecting arrays
31 sense terminals, 32 light entrances
33 condenser lenses, 34 optical fiber
35 cold light, 36 CCD arrays
361 bafflers detecting chambers 37
40 moving parts
Embodiment
See also Fig. 2, cold light instrument of the present invention comprises a detecting array 30, a plurality of optical fiber 34 in order to the transmission cold light signal, and the CCD array 36 of a reception cold light signal.This detects array 30, and it is in order to the sample that is carried in the trace dish 20 is detected, and it is provided with a plurality of sense terminals 31 towards the direction of trace dish 20, the number of those sense terminals and the spacing of arrangement thereof, can according to desire the samples tested container and be provided with.Be that detection with 96 holes (8 hole x 12 row) trace dish is an example, detecting array 30 comprises single-row 8 sense terminals 31 herein, and it can to coil 20 single-row 8 hole wells corresponding with trace.Detecting is during cold light, and all sense terminals 31 align mutually with corresponding hole well 21, carries out cold light in the hole well 21 and reacts the cold light 35 that produced and enter respectively this sense terminal 31 via the light entrance 32 of sense terminal 31 below ends.Cold light 35 is through a condenser lens 33 of being located at light entrance 32 tops, by means of its focussing force, cold light 35 is concentrated to import in the optical fiber 34 that sense terminal 31 tops are linked, respectively 34 of this optical fiber are respectively on the transmission block that respectively this cold light 35 to CCD arrays 36 are different, by this CCD array 36, can convert the light signal of different blocks on the CCD array 36 to electric signal numerical value respectively, or further be reduced into image signal, after then being converted to data again, can interpretation go out the respectively cold light numerical value of this sample.And detecting chamber 37 mainly is the space that is used for receiving optical fiber 34 and CCD array 36, and wherein, optical fiber 34 can extend according to the design requirement of instrument or is tortuous.Though optical fiber 34 can be close to the cold light signal that sample to be tested sent conduction completely, and be not subjected to ectocine, but shine the light arround trace dish 20, still the cold light signal that can disturb this sample to be tested to send, therefore detecting array 30 detecting trace coiled 20 o'clock in the embodiment of the invention, carry out under the lighttight environment of Ying Zaiyi, therefore can be in those assembly outer covers with an enclosing cover (not shown), to guarantee the accuracy of cold light detecting.
In addition, see also Fig. 3, this figure is the synoptic diagram that CCD array 36 receives the light signal.On this CCD array 36 baffler 361 can be set, this baffler 361 can make the input area of each adjacent optical fiber 34 on the CCD array 36 be able to be separated, to avoid the phase mutual interference between each input signal.During optical fiber 34 transmission is that the cold light signal that sense terminal 31 is transmitted directly is sent to each respective block on the CCD array 36, and writes down those cold light signals by the CCD of this respective block respectively.The present invention can comprise further that one handles synchronously the signal unit, uses and handles the signal that each pixel of different blocks is transmitted on the CCD array 36 simultaneously, again those signals is converted to data thereafter, further the interpretation cold light numerical value of this sample respectively.
Fig. 4 is a synoptic diagram of the invention process, the situation when shown in this figure being detecting 96 holes trace dish, and detecting array 30 is linked to a moving part 40 among the figure, does moving of single axial by these moving part 40 drive detecting arrays 30.In this detecting array 30 and be provided with 8 sense terminals 31,31 of those sense terminals are corresponding with the hole well 21 of longitudinal axis row on the trace dish 20 respectively.Can detect 8 samples of a longitudinal axis row simultaneously when detecting array 30 is done X axis and moved, moving and measure 12 times in regular turn can finish this trace dish detecting.Owing to sense terminal number among the present invention or setting at interval are to cooperate the hole well specification of trace dish to be measured to change, therefore in the detecting process, the longitudinal axis row or the transverse axis row of trace dish can be once detected,, the former eighth time can be saved if according to shown in the present embodiment.On the other hand, because optical sensor can capture a plurality of smooth signals simultaneously, therefore also can obtain a plurality of sample numerical value simultaneously, its whole detecting efficient is excellent than known technology.
Claims (11)
1. a sensor array type cold light instrument is characterized in that, this cold light instrument comprises at least:
One detecting array, this detecting array comprises a plurality of sense terminals, it is to be used to capture the cold light signal that sample to be tested sends;
A plurality of smooth signal transmission assemblies, it is to extend certainly respectively this sense terminal respectively, uses this cold light signal of transmission; And
One optical sensing subassembly, it is in order to receiving a plurality of cold light signals that those light signal transmission assemblies are transmitted, but and convert thereof into the respectively data of this sample to be tested cold light signal of identification;
This optical sensing subassembly is provided with baffler, uses isolation and respectively imports the mutual interference of cold light signal.
2. sensor array type cold light instrument as claimed in claim 1 wherein respectively is respectively equipped with a focalizer in this sense terminal, use the cold light that each sample is sent be focused to this light signal transmission assembly respectively end.
3. sensor array type cold light instrument as claimed in claim 2, wherein said focalizer are lens.
4. sensor array type cold light instrument as claimed in claim 1, wherein this light signal transmission assembly is an optical fiber.
5. sensor array type cold light instrument as claimed in claim 1, wherein this optical sensing subassembly is a Charged Coupled Device.
6. sensor array type cold light instrument as claimed in claim 1, wherein this optical sensing subassembly is a complementary matal-oxide semiconductor.
7. sensor array type cold light instrument as claimed in claim 1, wherein this optical sensing subassembly is an avalanche diode.
8. sensor array type cold light instrument according to claim 1, wherein this cold light instrument further comprises a moving part, by this moving part, can make this detecting array do moving of single axial.
9. sensor array type cold light instrument according to claim 1, but data that wherein should this sample to be tested cold light signal of identification is an electric signal.
10. sensor array type cold light instrument according to claim 1, but data that wherein should this sample to be tested cold light signal of identification is an image signal.
11. cold light instrument as claimed in claim 1, wherein this cold light instrument comprises that further one handles the signal unit synchronously, uses and handles the signal that different pixels transmitted on this optical sensing subassembly simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004101029164A CN100520355C (en) | 2004-12-28 | 2004-12-28 | Sensor array type cold light instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004101029164A CN100520355C (en) | 2004-12-28 | 2004-12-28 | Sensor array type cold light instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1796974A CN1796974A (en) | 2006-07-05 |
| CN100520355C true CN100520355C (en) | 2009-07-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004101029164A Expired - Fee Related CN100520355C (en) | 2004-12-28 | 2004-12-28 | Sensor array type cold light instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100520355C (en) |
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2004
- 2004-12-28 CN CNB2004101029164A patent/CN100520355C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1796974A (en) | 2006-07-05 |
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