CN103674934B - Micro-fluidic chip Image-forming instrument and system for chemiluminescence detection - Google Patents
Micro-fluidic chip Image-forming instrument and system for chemiluminescence detection Download PDFInfo
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- CN103674934B CN103674934B CN201310503116.2A CN201310503116A CN103674934B CN 103674934 B CN103674934 B CN 103674934B CN 201310503116 A CN201310503116 A CN 201310503116A CN 103674934 B CN103674934 B CN 103674934B
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- 238000002038 chemiluminescence detection Methods 0.000 title claims abstract description 23
- 238000001514 detection method Methods 0.000 claims abstract description 37
- 230000003287 optical effect Effects 0.000 claims abstract description 25
- 238000000034 method Methods 0.000 claims description 16
- 238000003384 imaging method Methods 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 8
- 229940088597 hormone Drugs 0.000 description 6
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- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 102000009151 Luteinizing Hormone Human genes 0.000 description 2
- 108010073521 Luteinizing Hormone Proteins 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
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- 230000003053 immunization Effects 0.000 description 2
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- 238000000504 luminescence detection Methods 0.000 description 2
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- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 102000003946 Prolactin Human genes 0.000 description 1
- 108010057464 Prolactin Proteins 0.000 description 1
- 102000011923 Thyrotropin Human genes 0.000 description 1
- 108010061174 Thyrotropin Proteins 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 238000003759 clinical diagnosis Methods 0.000 description 1
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- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- 230000001748 thyrotropin Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Abstract
The invention discloses a kind of micro-fluidic chip Image-forming instrument and system for chemiluminescence detection, the instrument includes apparatus frame and the light path stuck-module on the apparatus frame, chip support structures, illuminator and optical lens group and detection module, the light path stuck-module is used for fixing the position of optical lens group and the adjustment of light path and fixation, the chip support structures are used for supporting and moving chip is to adjust chip position, and the illuminator and optical lens group are used for collecting the chemiluminescence signal on chip and focusing on the detection module；The detection module is used for receiving chemiluminescence signal and being changed into analog electrical signal, then analog electrical signal is changed into digital electric signal.The instrument connection computer constitutes system.The instrument can carry out effectively collecting and analyzing to the chemiluminescence signal on micro-fluidic chip, and the equipment instrument is little, is a kind of new portable micro-fluidic chip analyzer.
Description
Technical field
The present invention relates to microfluidic chip system technical field, and in particular to a kind of for the micro- of chemiluminescence detection
Fluidic chip Image-forming instrument and system.
Background technology
Microfluidic analysis method is the technology that new development in recent years is got up, the method based on analytical chemistry, in conjunction with
The achievement of the related disciplines such as biochemistry, molecular biology, physical chemistry and immunology, in micron scale construction, manipulation is nanoliter extremely
Picoliters volume fluid, is analyzed detection to test substance, and with small volume, specific surface area is big, the response time is short, analyze speed
Hurry up, reagent and amount of samples is few and Multi-example multi objective is while the advantages of detecting.To relevant stigmata in clinical disease research
There is important practical to be worth for the discovery of thing and disease early diagnosiss.When conventional diagnostic analysis method needs long analysis
Between, Liquid-treatment processes are also cumbersome, and flux is smaller（One or several samples can only be detected every time）, and need to compare
More antibody reagent.And microfluidic analysis chip then can be efficiently against these shortcomings.For example, in analysis sample size very
During few sample, microflow control technique shows extremely strong advantage, it usually needs sample size is that several milliliters of experiment is adopting miniflow
After control technology, it is only necessary to several microlitres of sample size, the consumption of sample and reagent is greatlyd save.In clinical immunization detection neck
Domain, the combination of microfluidic analysis technology and immunoassay, can also overcome to a certain extent traditional immunization analysis other lack
Point, therefore, has caused the extensive concern of clinical laboratory medicine in recent years.Microflow control technique is applied to immune detection can be greatly
Promote the raising of immunoassay technology, be with a wide range of applications and important using value.
But, in current Microfluidic Immunoassay method, although micro-fluidic chip volume very little, is matched somebody with somebody with micro-fluidic chip
The detecting instrument volume of set is general very big, is not easy to the extensive application and popularization of micro-fluidic detection method.And can not body completely
The existing micro-fluidic advantage in easy quick detection this aspect.It is primarily due to also detect for micro-fluidic chip without special
Instrument, therefore, it is necessary to develop a kind of small-sized micro-fluidic chip analyzer device, realize that what sampling and analysis of signal processed has
Machine is integrated, and is miniaturized microflow control technique, easy.
Chemiluminescence is generated from a kind of light radiation in chemical reaction process, and chemiluminometry is sent out by chemistry
Optical phenomenon and a kind of analysis method for setting up, the method do not need light source and dispersion means, without optical analysis method in
The interference of common scattered light and veiling glare, substantially increases signal to noise ratio, thus with specificity is good, that the range of linearity is wide is excellent
Point, oneself is widely used in the every field such as life sciences, clinical medicine, environment measuring in recent years.Immune detection in hospital
In, microwell plate chemiluminescence method has become the method for predominantly detecting, and in clinical diagnosises work plays huge effect.Will
Microfluidic chip technology can be effectively reduced micro-fluidic chip after being combined with chemical luminescence detection method and detection means is wanted
Ask, reduce the volume of detection means, be easy to the popularization and application of microfluidic chip technology.Therefore, this area is required to miniflow
The miniature instrument dedicated for micro-fluidic chip detection combined with chemical luminescence detection method by control chip technology.
Content of the invention
The present invention provides a kind of micro-fluidic chip Image-forming instrument and system for chemiluminescence detection, can be to micro-fluidic
Chemiluminescence signal on chip carries out effectively collecting and analyzing, and the equipment instrument is little, is a kind of new portable micro-fluidic
Chip analyzer.
The present invention provides technical scheme below：
A kind of micro-fluidic chip Image-forming instrument for chemiluminescence detection, including apparatus frame and is arranged on the instrument
Light path stuck-module, chip support structures, illuminator and optical lens group and detection module on framework, the light path stent
Block is used for fixing the position of optical lens group and the adjustment of light path and fixation, and the chip support structures are used for supporting and mobile core
To adjust chip position, the illuminator and optical lens group are used for collecting the chemiluminescence signal on chip and focusing on piece
The detection module；The detection module is used for receiving chemiluminescence signal and being changed into analog electrical signal, then by analog telecommunications
Number it is changed into digital electric signal.
Preferably, the chip support structures be chip tray, more preferably plug-type chip tray.
Preferably, the detection module is CCD camera.
Preferably, the CCD camera is horizontally disposed with.
Preferably, the apparatus frame is light closed structure.
Preferably, the Image-forming instrument also includes Switching Power Supply.
Preferably, the detection module connects computer, and the computer is received and processed from the detection module
Digital electric signal.
Preferably, the Image-forming instrument is in integrally cuboid.
Preferably, the length of three adjacent edges of Image-forming instrument of the cuboid be respectively 12-18cm, 12-18cm and
35-45cm, preferably 15cm, 15cm and 40cm.
A kind of micro-fluidic chip imaging system for chemiluminescence detection, examines for chemiluminescence including as above
The micro-fluidic chip Image-forming instrument and connected computer of survey.
Beneficial effects of the present invention are：The micro-fluidic chip Image-forming instrument for chemiluminescence detection of the present invention, including
Apparatus frame and the light path stuck-module on the apparatus frame, chip support structures, illuminator and optical lens group
And detection module, the light path stuck-module be used for fix the position of optical lens group and the adjustment of light path and fixation, the core
Piece support structures are used for supporting and moving chip is to adjust chip position, and the illuminator and optical lens group are used for chip
Chemiluminescence signal collect and focus on the detection module；The detection module is used for receiving chemiluminescence signal and changing
For analog electrical signal, then analog electrical signal is changed into digital electric signal.The Image-forming instrument of said structure can be to micro-fluidic core
Chemiluminescence signal on piece carries out effectively collecting and analyzing.Due to being the imaging for being exclusively used in fluidic chip chemiluminescence signal
Instrument, does not have other uncorrelated parts in addition to above-mentioned critical piece；And due to viewing area relatively small, it is not necessary to adjust
Jiao, light path are less；Illuminator changes optical path direction and causes detection module（CCD camera）Can be horizontally disposed with, reduce instrument
Whole height, therefore the equipment instrument is little, is a kind of new portable micro-fluidic chip analyzer.
Description of the drawings
Fig. 1 is the structural representation of the micro-fluidic chip imaging system for chemiluminescence detection of the present invention, wherein, 1
Apparatus frame is represented, 2 represent light path stuck-module, and 3 represent chip tray, and 4 represent illuminator, and 5 represent optical lens group, 6 tables
Show CCD camera, 7 represent Switching Power Supply, and 8 represent computer.
Fig. 2 is that chemiluminescence signal light path is shown in the micro-fluidic chip Image-forming instrument for chemiluminescence detection of the invention
It is intended to, wherein, 1 represents apparatus frame, and 2 represent light path stuck-module, and 3 represent chip tray, and 4 represent illuminator, and 5 represent optics
Lens group, 6 represent CCD camera, and 7 represent Switching Power Supply, and 9 represent micro-fluidic chip.
Fig. 3 is the micro-fluidic immune detection that the micro-fluidic chip imaging system for chemiluminescence detection of the present invention reads
As a result photo, wherein white square represent chemiluminescence signal, and white square is brighter to represent that chemiluminescence signal is stronger.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment.Those skilled in the art will manage
Solution, following examples are only the preferred embodiments of the present invention, in order to more fully understand the present invention, thus should not be regarded as limiting this
The scope of invention.For a person skilled in the art, the present invention can have various modifications and variations, all essences in the present invention
Within god and principle, any modification, equivalent or improvement for being made etc. should be included within the scope of the present invention.
Fig. 1 and Fig. 2 is refer to, the micro-fluidic chip imaging system for chemiluminescence detection includes Image-forming instrument and calculating
Machine 8.Wherein the instrument mainly by apparatus frame 1, light path stuck-module 2, chip tray 3, illuminator 4 and optical lens group 5,
CCD（Charge-coupled Device, charge coupled cell）Camera 6 and Switching Power Supply 7.Wherein, apparatus frame 1 is used for instrument
The fixation of device each module position；Light path stuck-module 2 is used for fixing the position of optical lens group 5 and the adjustment of light path and consolidates
Fixed；Chip tray 3 is used for moving chip, and adjustment chip position, makes the chemiluminescence signal region of chip be placed in detection system
The underface of the observation area of system；Illuminator 4 and optical lens group 5 are used for the chemiluminescence signal collection on chip, CCD phases
Machine 6；The optical signalling for obtaining is changed into analog electrical signal by CCD camera 6, then analog electrical signal is changed into digital electric signal biography
It is defeated by computer 8.The system achieves the integrated of detecting system, and the system meets carries out chemiluminescence letter to micro-fluidic chip 9
The requirement of number detection, small volume is a kind of new portable micro-fluidic chip analyzer, has emphatically in microfluidic analysis field
The application potential that wants.
The chip tray 3 other chip support structures can be substituted with chip support plate etc., but the present invention preferably core
Tablet tray 3, and particularly preferably plug-type chip tray, this plug-type chip tray cause picking and placeing all very just for chip
Chip tray only need to be pulled out and be placed on it chip, then be pushed it into by victory.The CCD camera 6 can be examined with other
Survey module to replace, as long as the detection module can receive chemiluminescence signal and be changed into analog electrical signal again by analog electrical signal
It is changed into digital electric signal, the present invention preferably CCD camera, it is also possible to referred to as ccd image sensor, is a kind of semiconductor device,
Optical image can be converted into digital signal, complete on micro-fluidic chip the imaging of chemiluminescence signal and be transferred to calculating
Machine.6 small volume of CCD camera of the present invention, and be horizontally disposed with, therefore reduce the whole height of instrument.Chemiluminescence signal
Collection needs light protected environment, and to exclude the interference of light in environment, therefore apparatus frame is light closed structure.The imager of the present invention
Device is in integrally cuboid, and the length of three adjacent edges can be 12-18cm, 12-18cm and 35-45cm, such as width respectively
Can be 12cm, 13cm, 14cm, 15cm, 16cm, 17cm or 18cm, preferably 15cm；Height can be 12cm, 13cm, 14cm,
15cm, 16cm, 17cm or 18cm, preferably 15cm；Length can be 35cm, 37cm, 39cm, 41cm, 43cm or 45cm, preferably
40cm.As can be seen that the Image-forming instrument is very compact, it is easy to use and carries.
The present invention micro-fluidic chip imaging system operation principle and process be：Chip tray 3 is pulled out, will be micro-fluidic
Chip 9 is placed on pallet, pushes chip tray 3, it is to avoid external light influence；The chemiluminescence signal of micro-fluidic chip 9 is by anti-
Light microscopic 4 is collected by optical lens group 5 after reflecting；CCD camera 6 is emitted onto the optical signalling on CCD chip surface and changes
For electrical signal, and then it is transferred to computer 8 and carries out data processing and analysis.
Through test, the micro-fluidic chip Image-forming instrument has higher resolution and sensitivity, disclosure satisfy that in miniflow
The needs of chemiluminescence method detection are carried out on control chip；Simple and compact for structure, the small volume of instrument, easy to carry and use.
Micro-fluidic chemiluminescence detection system has following characteristics：Chip tray is designed using push-and-pull, and photophobism is good, and chip is placed and taken out
Convenient, while the locus of chip accurately can be determined, it is easy to detect；Using illuminator, it is that light path occurs right angle transformation, can
So that CCD camera horizontal positioned, utilization space to the full extent；System design is simple, and processing and manufacturing is with low cost；Achieve inspection
Integrated, the small volume of examining system, is convenient for Site Detection.
The chemiluminescence signal that micro-fluidic chip is detected using the micro-fluidic chip imaging system.Specifically, micro-fluidic
Thyrotropin is fixed on the direction of the 1 of chip, 2,3,4 and 5 respectively in advance（TSH）, prolactin antagonist（PRL）, interstitialcellstimulating hormone (ICSH)
（LH）, growth hormone（GH）And follicule-stimulating hormone (FSH)（FSH）Coated antibody；In five Micro-flow pipes of A, B, C, D and E successively
It is passed through confining liquid（BSA etc.）, hormone（Above-mentioned 5 kinds of hormones）, cleanout fluid（Phosphate buffer etc.）, mixing swash
Plain detection antibody（The antibody mixed liquor of above-mentioned 5 kinds of hormones）, cleanout fluid and chemical luminescence for liquid（Hydrogen peroxide and luminol etc.）, its
The hormone each led in five Micro-flow pipes of middle A, B, C, D and E is TSH, PRL, LH, GH and FSH.The photo of reading（Figure
3）Show：Above-mentioned 5 kinds of hormones can pass through its specificity coated antibody capture, and detected antibody test is arrived, white side in figure
Block represents chemiluminescence signal, and white square is brighter to represent that chemiluminescence signal is stronger.
Applicant states that the present invention illustrates the detailed features and method detailed of the present invention by above-described embodiment, but
Above-mentioned detailed features and method detailed is the invention is not limited in, that is, does not mean that the present invention has to rely on above-mentioned detailed features
And method detailed could be implemented.Person of ordinary skill in the field it will be clearly understood that any improvement in the present invention, to this
The interpolation of the equivalence replacement of bright selection component and auxiliary element, selection of concrete mode etc., all fall within protection scope of the present invention
Within the scope of disclosure.
Claims (9)
1. a kind of micro-fluidic chip Image-forming instrument for chemiluminescence detection, including apparatus frame and is arranged on the instrument frame
Detection module on frame；Wherein, the apparatus frame is light closed structure；Characterized in that, also including installed in the instrument
Light path stuck-module, plug-type chip tray, illuminator and optical lens group on framework；Wherein,
Illuminator, optical lens group, light path stuck-module and detection module are located at the top of the interior spatial structure of apparatus frame；
Also, the position of illuminator, optical lens group, light path stuck-module and detection module is from left to right to be sequentially placed；
The light path stuck-module is used for fixing the position of optical lens group and the adjustment of light path and fixation；
The plug-type chip tray is located at the lower section of apparatus frame, for support and moving chip to adjust chip position, with
The chemiluminescence signal region of chip is made to be placed in the underface of observation area；
The illuminator and optical lens group are used for collecting the chemiluminescence signal on chip and focusing on the detection module；
Wherein, after the chemiluminescence signal of chip is by mirror reflection, it is collected by optical lens group；
The detection module is used for receiving chemiluminescence signal and being changed into analog electrical signal, then analog electrical signal is changed into number
The word signal of telecommunication.
2. the micro-fluidic chip Image-forming instrument for chemiluminescence detection according to claim 1, it is characterised in that described
Detection module is CCD camera.
3. the micro-fluidic chip Image-forming instrument for chemiluminescence detection according to claim 2, it is characterised in that described
CCD camera is horizontally disposed with.
4. the micro-fluidic chip Image-forming instrument for chemiluminescence detection according to claim 1, it is characterised in that also wrap
Include Switching Power Supply.
5. the micro-fluidic chip Image-forming instrument for chemiluminescence detection according to claim 1, it is characterised in that described
Detection module connects computer, and the computer is received and processes the digital electric signal from the detection module.
6. the micro-fluidic chip Image-forming instrument for chemiluminescence detection according to any one of claim 1-5, its are special
Levy and be, overall is in cuboid.
7. the micro-fluidic chip Image-forming instrument for chemiluminescence detection according to claim 6, it is characterised in that described
The length of three adjacent edges of cuboid is 12-18cm, 12-18cm and 35-45cm respectively.
8. the micro-fluidic chip Image-forming instrument for chemiluminescence detection according to claim 7, it is characterised in that described
The length of three adjacent edges of cuboid is 15cm, 15cm and 40cm respectively.
9. a kind of micro-fluidic chip imaging system for chemiluminescence detection, including as described in any one of claim 1-8
Micro-fluidic chip Image-forming instrument and connected computer for chemiluminescence detection.
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| CN201310503116.2A CN103674934B (en) | 2013-10-23 | 2013-10-23 | Micro-fluidic chip Image-forming instrument and system for chemiluminescence detection |
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| CN201310503116.2A CN103674934B (en) | 2013-10-23 | 2013-10-23 | Micro-fluidic chip Image-forming instrument and system for chemiluminescence detection |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105388131A (en) * | 2014-09-09 | 2016-03-09 | 国家纳米科学中心 | Fluorescence detection instrument and system based on micro-fluidic chip |
| CN104535547B (en) * | 2014-11-29 | 2017-04-05 | 北京工业大学 | A kind of common focused light passages portable unit of observation micro-fluidic chip |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201859122U (en) * | 2010-09-13 | 2011-06-08 | 北京赛智创业科技有限公司 | Integrated micro chemical light-emitting imager |
| CN202049111U (en) * | 2011-01-25 | 2011-11-23 | 山东师范大学 | Laser-induced fluorescent detector for micro-fluidic analysis |
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2013
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201859122U (en) * | 2010-09-13 | 2011-06-08 | 北京赛智创业科技有限公司 | Integrated micro chemical light-emitting imager |
| CN202049111U (en) * | 2011-01-25 | 2011-11-23 | 山东师范大学 | Laser-induced fluorescent detector for micro-fluidic analysis |
Non-Patent Citations (2)
| Title |
|---|
| Microfluidic biochip for chemiluminescent detection of allergen-specific antibodies;Kecin A.Heyries 等;《Biosensors and Bioelectronics》;20080307;1802-1808 * |
| 微流控芯片化学发光检测系统的关键技术研究;卢绍永;《中国优秀硕士学位论文全文数据库 信息科技辑》;20111215;正文18-33页 * |
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