CN100496221C - Authentication method of hybrid muskmelon - Google Patents
Authentication method of hybrid muskmelon Download PDFInfo
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- CN100496221C CN100496221C CNB2006100242400A CN200610024240A CN100496221C CN 100496221 C CN100496221 C CN 100496221C CN B2006100242400 A CNB2006100242400 A CN B2006100242400A CN 200610024240 A CN200610024240 A CN 200610024240A CN 100496221 C CN100496221 C CN 100496221C
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Abstract
A method for discriminating the hybrid muskmelon plant includes such steps as transferring the white stem character of muskmelon plant into the female parent to make it express the white stem character, hybridizing between the female parent with white stem character and the male parent with green stem character to obtain the first hybrid generation, sowing, germinating, and observing the seedlings to discriminate the hybrid seedlings whose hypocotyl shows green from the non-hybrid ones whose hypocotyl shows white.
Description
Technical field
This invention relates to the authentication method of muskmelon cross-fertilize seed, especially utilizes the method for white stem character test muskmelon cross-fertilize seed.
Background technology
At present, muskmelon first generation of hybrid kind has been widely used in production, but in artificial seed's process; owing to isolating tight or artificial emasculation is untimely, reason such as not thorough, often be mixed with the seed of maternal inbred line and cause damage, therefore to production; crossbreed must pass through purity detecting before packing and selling.Current, there are following three kinds of methods in seed production both at home and abroad and operating division to what the detection of muskmelon cross-fertilize seed purity was mainly adopted:
1, planting method: the muskmelon cross-fertilize seed that will gather then, sampling (in the greenhouse or strange land) growing and cultivation in the environment of suitable planting according to the difference of this botany of father and mother proterties and the genetic development of proterties, is identified the purity of crossbreed.The shape that mainly contains leaf margin is (as the anodontia female parent of doing of leaf margin, the leaf margin sharp mouth is done male parent, F1 is for being paternal character), the climing feature of stem (is done female parent as the climing smooth nothing hair of stem, have bristle to do male parent, F1 is for being paternal character), the color of the leather of young melon (is done female parent as light color, do male parent for dark or wounded in the battle, F1 is for being paternal character) etc.Advantage: though this method is more intuitive and reliable, technology is easy, but operate in a large number, but be difficult for EARLY RECOGNITION, and floor space is big, and the time is long, and is subjected to the amblent air temperature condition restriction, and cost is also higher.
2, isodynamic enzyme or protein act: mainly be to utilize polyacrylamide gel or starch-gel electrophoresis technology, by analyzing the purity of identifying muskmelon cross-fertilize seed as the protein and the isodynamic enzyme of gene outcome.This method is set up the isozymogram or the albumen band of muskmelon cross-fertilize seed and this standard of father and mother earlier by analysis, again as contrast, the crossbreed sample is analyzed.The advantage of this method is to carry out indoor, but EARLY RECOGNITION, relatively accurately.But technology operation is strict, and the program complexity discern not intuitively, and repeatability is good inadequately sometimes, requires certain laboratory condition, difficult great amount of samples is analyzed.
3, DNA analysis method: according to molecular biological principle, directly on dna level the muskmelon first generation of hybrid and his father's female parent are carried out the genome difference analysis, the technological means that adopts mainly contains RAPD, RFLP, SSR etc. at present.Compare with Isozyme Analysis with protein, the method for DNA analysis more can reflect the difference of genomic DNA between kind.The advantage of this method is to carry out indoor, but EARLY RECOGNITION, the accuracy height, intuitive is strong, good reproducibility.But the same operation of this technology is strict, and the program complexity needs laboratory condition preferably, and cost is also higher relatively, also is difficult for to the great amount of samples analysis also extensive use aborning at present.
Summary of the invention
Technical problem to be solved by this invention is to simplify the method that muskmelon cross-fertilize seed is identified, shortens and identifies required time, improves and identifies accuracy and promptness.
In order to solve the problems of the technologies described above, the technical solution used in the present invention has been utilized the white stem proterties of muskmelon, in the seedling phase muskmelon cross-fertilize seed is identified.Concrete steps are the female parents that earlier the white stem proterties of muskmelon changed over to hybrid combination, make the white stem proterties of maternal performance, hybridize with male parent again and obtain first generation of hybrid seed with green stem proterties, then with first generation of hybrid planting seed, observe after emerging, after emerging 2～4 days, hypocotyl is not a crossbreed for the individual plant of white, and hypocotyl is a crossbreed for green individual plant.
Describedly change the white stem proterties of muskmelon the method for hybrid combination female parent over to, can adopt cross method, also can adopt engineered method.
Beneficial effect of the present invention is:
Can intuitively discern in early days, can identify hybrid strain and non-hybrid strain, seed is circulated in early days in the muskmelon seedling phase.Method is simple for this, quick and precisely, is easy to penetration and promotion, and after seed put into production, the melon grower also can just can accurately discern and reject non-hybrid seedling when growing seedlings, and avoids because the seed hybrid rate brings economic loss to production inadequately; This method is not subject to environment and condition restriction, all can identify in indoor and outdoor, and equipment is simple, utilizes seedling-cultivating tray and simple facility just can finish, and cost is cheap, also is easy to great amount of samples is identified; This method accurately and reliably, the white stem proterties of muskmelon is a qualitative character, the seedling phase and after whole growth in the phase, hybrid seedling and non-hybrid seedling all have stable obvious difference, can identify at any time; This method is applied widely, not limited to by kind, to no any harmful effects such as the output of kind, qualities.
Description of drawings
Fig. 1 is the schematic diagram of muskmelon crossover process in the muskmelon cross-fertilize seed authentication method of the present invention.
Embodiment
Below in conjunction with accompanying drawing the specific embodiment among the present invention is described further.
The inventor has found to have white stem proterties from the muskmelon idioplasm resource of collecting individual plant has carried out selfing and has reserved seed for planting, and this proterties has been observed and hereditary capacity research continuously.Studies show that the white stem proterties of muskmelon is the recessive character by the pair of alleles control of heredity, has certain genetic development, be easy to change white stem proterties over to muskmelon maternal material and utilize by the method for hybridizing transformation.And possesses the muskmelon material of white stem proterties, just tangible difference is arranged from emerging with general muskmelon material, its hypocotyl is white in color, and the hypocotyl of general muskmelon material promptly transferred green in the same day of being unearthed, this difference remains to stopping of plant vegetative growth always, and therefore white stem also is suitable for the proterties that serves as a mark.
As shown in Figure 1, the parent with white stem proterties as female parent, is hybridized the parent with just evergreen stem and to be obtained first-filial generation (F1) as male parent, F1 again selfing obtain the second generation (F2), the individual plant segregation ratio of white stem and just evergreen stem is 1:3 among the F2; In F1 and the white stem parent backcross progeny, the individual plant segregation ratio of white stem and just evergreen stem is 1:1, shifts the white stem muskmelon strain that seed selection is new with this hybridization that can carry out white stem proterties.Parent and after having parent's hybridization of just evergreen stem proterties with white stem proterties, white stem proterties disappearance in first-filial generation (F1) individual plant, plant shows as just evergreen stem, and the stem of non-crossbreed individual plant is white in color, and can detect muskmelon seeds purity or hybrid rate with this.
Concrete computing formula is as follows:
Employing possesses the improved seeds of the white stem proterties of muskmelon and does the apolegamy that female parent carries out hybrid combination, or the white stem proterties of muskmelon transferred on the female parent of certain successful hybrid combination by general cross or technique for gene engineering method, such first generation of hybrid hybrid individual plant can be realized accurate evaluation in the seedling phase (emerge back 2～4 days in).Non-crossbreed individual plant just has obvious difference with the crossbreed individual plant from the beginning of emerging, the non-crossbreed seedling hypocotyl proterties that is white in color, and crossbreed seedling hypocotyl is green proterties, this difference remains to stopping of plant vegetative growth always.
Seed selection also utilizes the white stem strain of good muskmelon M55-3, its fruit ellipse, and white smooth bark, under booth stand cultivation condition, about mean fruit weight 2kg, the pulp light red orange, the crisp exquisiteness of meat more than center refractive power sugar 16 degree, has stronger coordinate force.Utilize the new hybrid combination FM541 of its apolegamy, the fruit ellipse, white smooth bark is under booth stand cultivation condition, about mean fruit weight 1.5kg, pulp Chinese red, the crisp exquisiteness of meat, more than center refractive power sugar 17 degree, the field resistance performance is good, has vast market prospect.
Claims (4)
1, a kind of method of identifying muskmelon cross-fertilize seed, it is characterized in that: the female parent that the white stem proterties of muskmelon is changed over to hybrid combination, make female parent show white stem proterties, obtain the first generation of hybrid with maternal with paternal hybrid with green stem proterties, after the sowing first generation of hybrid is emerged, the first generation of hybrid is observed, and the individual plant that stem is white in color after emerging 2～4 days is not a crossbreed, and the greeny individual plant of stem is a crossbreed.
2, a kind of method of identifying muskmelon cross-fertilize seed according to claim 1 is characterized in that: utilize hybridization or engineered method that white stem proterties is changed in the female parent.
3, a kind of method of identifying muskmelon cross-fertilize seed according to claim 1 is characterized in that: described white stem proterties is the recessive character by the pair of alleles control of heredity.
4, according to claim 1,2 or 3 described a kind of methods of identifying muskmelon cross-fertilize seed, it is characterized in that: described white stem proterties is in the seedling phase proterties that is white in color of hypocotyl.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100242400A CN100496221C (en) | 2006-02-28 | 2006-02-28 | Authentication method of hybrid muskmelon |
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| CNB2006100242400A CN100496221C (en) | 2006-02-28 | 2006-02-28 | Authentication method of hybrid muskmelon |
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| CN101027963A CN101027963A (en) | 2007-09-05 |
| CN100496221C true CN100496221C (en) | 2009-06-10 |
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| CNB2006100242400A Expired - Fee Related CN100496221C (en) | 2006-02-28 | 2006-02-28 | Authentication method of hybrid muskmelon |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106718850A (en) * | 2017-01-22 | 2017-05-31 | 河南农业大学 | A kind of breeding method of muskmelon cenospecies |
| CN113207679A (en) * | 2021-05-21 | 2021-08-06 | 上海市农业科学院 | Identification method for purity of muskmelon hybrid |
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Non-Patent Citations (4)
| Title |
|---|
| 响水地区盐碱土壤大棚甜瓜品比试验. 周萍.上海蔬菜，第4期. 2005 |
| 响水地区盐碱土壤大棚甜瓜品比试验. 周萍.上海蔬菜，第4期. 2005 * |
| 响水盐碱土壤大棚甜瓜品比试验. 龚文兵等.现代种业，第1期. 2002 |
| 响水盐碱土壤大棚甜瓜品比试验. 龚文兵等.现代种业，第1期. 2002 * |
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Effective date of registration: 20100623 Address after: 201106 No. 35 Nanhua Road, Shanghai, Minhang District Co-patentee after: Shanghai Kelite Agricultural Science (Group) Co., Ltd. Patentee after: Inst. of Gardening, Academy of Agriculture of Shanghai Co-patentee after: Shanghai Keyuan Seed Co., Ltd. Address before: 201106 No. 35 Nanhua Road, Shanghai, Minhang District Patentee before: Inst. of Gardening, Academy of Agriculture of Shanghai |
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