CN108308018A - Phosphorus efficiency kind is cultivated using soybean root dry mass near isogenic lines（System）Method - Google Patents
Phosphorus efficiency kind is cultivated using soybean root dry mass near isogenic lines（System）Method Download PDFInfo
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Abstract
The invention discloses cultivate phosphorus efficiency kind using soybean root dry mass near isogenic lines（System）Method, belong to crops phosphorus nutrition genetic improvement utilize field.This method includes phosphorus efficiency related root traits recombinant inbred lines（RILs）Build, efficient root dry mass near isogenic lines（NILs）Structure, phosphorus efficiency QTLs（Quantitative trait loci）Hybridization transfer screening etc. key steps.The present invention is without relying on the gene cloning and transgenic technology that time-consuming, cost is high, phenomena such as overcoming the Phenotypic Selection difficulty and Linkage drag that conventional cross-breeding is brought simultaneously, realize phosphorus nutrition efficiently, the quick integration of high yield, Fineness gene, efficiently, accurately obtain that root dry mass is excellent, soybean varieties of phosphorus efficiency（System）, increase substantially the breeding efficiency of phosphorus efficiency kind.
Description
Technical field
The present invention designs crops phosphorus nutrition genetic improvement and utilizes field, and in particular to utilizes soybean root dry mass near isogene
Cultivate phosphorus efficiency kind（System）Method.
Background technology
The soybean varieties for cultivating and promoting phosphorus efficiency have important ecology and economic implications.The cultivated soybean (Glycine max(L.)Merrill) industrial crops are important, it is one of the main source of edible oil and Seed Storage Protein, growth is extensive
Soil environment in.Phosphorus is one of crop nutrition content three elements.For growing plant in the soil, phosphorus is limited mineral battalion
It supports.It depends on soil characteristic and unstable phosphorus content using ability.It is estimated that in the cultivating soil in the world 2/3,
Available phosphorus is generally relatively low, and there are different degrees of scarce phosphorus phenomenons.Lack phosphorus when, protein biosynthesis block, new cytoplasm and
Nucleus forms less, influences cell division, slow-growing, and branch or tiller are reduced, and plant is short and small, and florescence and maturity period are all
Delay, yield reduce, and resistance weakens.In South China tropical and subtropical region, soil available phosphorus content is low, utilization rate is not high, sternly
Ghost image rings the yield and quality of soybean.By largely using phosphate fertilizer in agricultural production in traditional plantation, yield, this side are improved
Method has resulted in the crisis of phosphor resource, and problem of environmental pollution also becomes increasingly conspicuous.To change this present situation, remedies, it is expected that
By the genetic improvement to soybean Phosphorus Nutrient Efficiency, enable to better adapt to specific ecological condition.Therefore, carry out soybean
The Screening of Germplasm of phosphorus efficiency cultivates the new soybean varieties of phosphorus efficiency, improves soybean activation and the ability using soil phophorus library,
It is the alternate resources of phosphorus ore to make soil phophorus Kucheng, has great ecology and economic implications.
Due to lacking Evaluation on specificity index, the upper common soybean phosphorus efficiency Screening of Germplasm method efficiency of production at present
It is relatively low.Plant is to the absorption of phosphorus and the relative size of Utilization ability with phosphorus efficiency（phosphorus efficiency）To indicate.
Soybean different cultivars（Genotype）There is different adaptation mechanisms to phosphorus shortage.It evaluates suitable under the conditions of different soybean varieties phosphorus lack
Answering property generally use germplasm screening method uses phosphorus shortage soil（Crop field or soil potting）Or to soybean germplasm under the conditions of water planting
Resource carries out plantation evaluation.These methods are required for occupying larger space, and generally require and complete a growth cycle, consumption
It is time-consuming longer, the accuracy of test result is influenced whether by factors such as soil, nutrient, moisture, weathers.Phosphorus efficiency kind is sieved
Choosing, determines that the index of soybean phosphorus efficiency is very important.The evaluation index of soybean growth phosphorus efficiency has very much, such as growth and development
Testing index, including root morphology and architecture, biomass, leaf area, leaf age relative value, photosynthesis characteristics, physiological and biochemical index, tiller number
（Rice）Deng.It was verified that not all parameter index is all effective for the evaluation and breeding of phosphorus efficiency soybean.Only
Only it is that some are easy to measure and is used in soybean breeder with the relevant parameter of phosphorus efficiency.Evaluation method packet statistically
Include single parameter, various parameter and principal component analysis method etc..The evaluation of single parameter has directly displayed different genotype
Compare root top biomass when phosphorus lacks, phosphorus absorbs and the ratio of phosphorus efficiency.These parameters are in phosphorus utilizability soil
Sensitive.Compared with single parameter display methods, various parameter evaluation such as kmeans cluster method is more superior, because of his comprehensive analysis
The various parameter of phosphorus efficiency.However, kmeans cluster method cannot illustrate contribution of the different parameters to phosphorus efficiency, in phosphorus efficiency rating,
Its result may be inaccurate.On this basis, it is thus proposed that a half-way house, i.e. principal component analysis method, by comprehensive
Total major part of calculating is in low-phosphorous condition（-P）And it is low-phosphorous（-P）And high phosphorus（+P）The standard value of the percentage of opposite value parameter obtains
It arrives, reservation is more than 1 characteristic value.Planting medium either soil or water planting, entire screening process process is more, expends the time
It grows, easily by environmental influence, and ripe difficult under the conditions of water planting, destructible raw material.Although being changed on statistical method
It is kind, but due to lacking effective Evaluation on specificity index, lead to of high cost, inefficiency, poor accuracy.
Root dry mass is the key index for evaluating soybean phosphorus efficiency.Root is that most of crop absorbs the unique of nutrient from soil
Approach.The development condition of root system and crop nutrient absorption are closely related.Crop root dry mass（root architecture）Refer to same
Different types of in one root system（The system of taproot）Or adventitious root（The system of fibrous root）Spatial modelling in somatomedin and distribution
（Lynch, Root architecture and plant productivity. Plant Physiolo. 1995,109:
7-13）.Root dry mass be described in advanced form level one of crop root 3 D stereo geometric configuration it is abstract, indirectly
Property aggregate concept, usually with root branch situation, lateral root distribution and the parameters such as root system geotropism come the root structure of intermediate description crop
Type feature, it mainly describes the quantity and spatial distribution characteristic that root system absorbs site or absorption area.In this sense
For, root morphology is included in root dry mass feature.Root system configuration is by genic control, also such as with rhizospheric environment condition
Phosphorus nutrient situation is related.It is to connect the mode of soil by root system as main source that root, which absorbs P elements,.Suction of the plant to phosphorus in soil
The available phosphorus for relying primarily on and being touched around root system absorption is received, long term growth is under low-phosphorus stress environmental condition, higher plant meeting
Some adaptation mechanisms absorbed to soil phophorus are formed, the differentiation of root morphology feature is included（As root hair is formed）, induction acid phosphatase
Enzyme, the formation of special root exudates and secretion, the formation of root dry mass etc., to influence phosphorus efficiency.Have many research cards
Bright, root traits absorb closely related with soybean phosphorus nutrition efficient.Under the conditions of low-phosphorous, phosphorus efficiency soybean can increase root and arrive
Root top dry ratio increases root long, increases lateral root, increase root approximate number amount, change root dry mass.Under the conditions of phosphorus lacks, phosphorus is high
It imitates genotype to be easier to accumulate biomass, increases root long, increase phosphorus absorbs and root top phosphorus utilization.Enter from Root morphology configuration
Hand is established to mould the Crop Root Morphology configuration breeding that " ideal root dry mass " is target, i.e. root system breeding, it is possible to take first
Obtain the breakthrough of mineral nutrition high-efficient breeding（Yan little Long and Zhang Fusuo, plant nutrient heredity, Beijing：Chinese agriculture publishing house,
2007）.Applicant team proposes to carry out soybean phosphorus efficiency genotype screening using the relevant root traits of phosphorus efficiency（Yin
First duckweed etc., using Root morphology configuration screen phosphorus efficiency soybean genotype, Molecular Plant Breeding, 2015,5（13）:999-
1008）, there is preferable screening effect using root traits indexs such as root biomass, root phosphorus content, root dry mass, but root system excavates
Larger with the workload of analysis, soybean root system excavates the damage for also resulting in plant, causes the inconvenience of practical operation.
The evaluation of soybean phosphorus efficiency can efficiently and be nondestructively carried out using phosphorus efficiency related root traits molecular labeling.It closes
The report of root system of plant character QTL positioning analysis sees early 1990s earliest under different phosphate nutritional condition.Simple sequence
Row repeat（Simple sequence repeat, SSR）It is a kind of DNA base sequence being widely present in eukaryotic gene groups,
They are by the basic sequence series unit of 1-6 base composition, and there are abundant differences for the repeat number between iso-allele.
For the size of soybean genome about between 1129 × 106 ~ 1181 × l06 bp, wherein 40%-60%'s is repetitive sequence, and 25% is
Moderately repetitive sequence, 20% is a small number of repetitive sequences.Soybean is shown to the comparison of the physical mapping and Molecular Markers Linkage Map of soybean
In each cM be roughly equivalent to the genome of 550 kb, therefore soybean genome genetic distance overall length about 3500cM.Since SSR is marked
Note is the codominant marker copied there is only one in genome, therefore on map construction, SSR marker is generally as collection of illustrative plates
Anchor marker, be conducive to the integration of the merger and different linkage groups of collection of illustrative plates linkage group or chromosome.Liang Quan etc.（Liang et
al., QTL analysis of root traits as related to phosphorus efficiency in
Soybean, Annals of Botany, 2010,106: 223-234）Result of study, it is found that B1, C1, D1a, D2 etc. are chain
There are 7 sites related to soybean phosphorus efficiency on group.
The characters such as Phosphorus Nutrition in Plants efficiency, root system configuration are quantitative character, easily by environmental influence.Therefore, traditional
Breeding method since often difficult give realizes effective transfer of the efficient character of phosphorus nutrition to Linkage drag.People have been cloned into so far
Some and root morphology and the relevant gene of phosphorus efficiency.With the efficiently relevant transgenic technology of phosphorus nutrition, although being conducive to quick, accurate
Really transfer phosphorus efficiency related gene, but since the acquisition of mutant material is difficult, time-consuming, costly for experiment in the technical method
It is high, and there is also the disputes of product risks for transgenic technology, and it is larger to promote and apply difficulty.
Invention content
In order to solve the above-mentioned technical problem, the present invention is that phosphorus nutrition absorbs and the major organs that utilize using soybean root system
Feature, it using soybean root dry mass near isogenic lines is that parent or intermediate materials cultivate soybean phosphorus efficiency kind to provide a kind of（System）'s
Method.
The technical solution that the present invention takes is：
Phosphorus efficiency kind is cultivated using soybean root dry mass near isogenic lines（System）Method, carry out according to the following steps：
（1）Using two geographical distant races and have confirmed the soybean varieties that there is significant difference in root dry mass, phosphorus efficiency etc.
Hybridization builds the recombinant inbred lines of root traits correlation phosphorus efficiency（RILs）, and apply SSR（Simple sequence repeats）Label is just fixed
Position soybean phosphorus efficiency target QTLs；
（2）In above-mentioned phosphorus efficiency target QTLs both sides encrypted indicias and polymorphism screening is carried out, it is final to obtain and phosphorus efficiency target
QTLs is closely chain, has the specific SSR marker of polymorphism；
（3）With step（1）In soybean phosphorus efficiency genotype be donor parents, using the kind of the inefficient product of phosphorus as recurrent parent, profit
Use step（2）In specific SSR marker screen and hybrid separation offspring selected in conjunction with phenotype, returned through 4~6 times
Friendship and molecular marker assisted selection, obtain the target QTLs of phosphorus efficiency containing soybean and genetic background and recurrent parent height are similar
Phosphorus efficiency root dry mass near isogenic lines（NILs）；
（4）With above-mentioned soybean phosphorus efficiency NILs for parent or intermediate materials, using domestic place or commercial variety as receptor parent into
Row sexual hybridization, by step（2）To hybridization, separation offspring carries out genome-wide screening and M8003 line, obtain root dry mass it is excellent,
The good new soybean varieties of phosphorus efficiency, Comprehensive Traits（System）.
Further, step（2）SSR closely chain the phosphorus efficiency target QTLs includes 21 pairs of SSR primer sequences, should
21 special SSR markers are, Sat_128, Sat_149, Satt519, Satt298, Sat_348, B1-5, B1-11, B1-18,
Sat_296、Sct_192、Satt458、Satt135、Sat_277、D2-2、D2-9、Sat_343、Satt507、Sct_010、
Satt076、Sat404、Satt042。
Further, step（2）The evaluation and screening soybean phosphorus efficiency Genotypic methods are:
（1）Control group is set, with BX10（Brazil 10）, 3 F10 generations recombinations from being RIL71, RIL97, RIL130 be phosphorus efficiency
Kind, BD2（It is No. 2 local）, 4 wild soybean YN01, YN02, YN03, YN04,3 F10 for recombinant inbred lines RIL32,
RIL144, RIL171 phosphorus inefficient cultivar；
（2）Separation and Extraction DNA in progeny soybean blade is detached from hybridization；
（3）Using DNA as template, it is primer with above-mentioned special SSR molecular marker, specific fragment is gone out by PCR amplification；
（4）Denaturing polyacrylamide gel electrophoresis（PAGE）Detection, amplified band carry out 0,1 matrix statistics, build SSR marker number
According to matrix；
（5）Genetic similarity and genetic cluster analysis are calculated according to amplified band, you can judge phosphorus efficiency soybean genotype.
Beneficial effects of the present invention are：
Method provided by the invention without relying on the gene cloning and transgenic technology that time-consuming, cost is high, but is educated with tradition
Kind mode builds root traits correlation RILs to position phosphorus efficiency with the big kind of two root dry mass, phosphorus efficiency variance first
QTL, then by means of building phosphorus efficiency NILs with SSR marker closely related phosphorus efficiency QTLs, finally with phosphorus efficiency root dry mass
Near isogenic lines be parent or intermediate materials, by with phosphorus efficiency target QTLs（Quantitative trait loci）Closely chain SSR points
Sub- marker assisted selection quickly, accurately shifts phosphorus efficiency QTLs, to realize that phosphorus nutrition is efficient, high yield, Fineness gene quick
It integrates, efficiently and accurately obtains that root dry mass is excellent, soybean varieties of phosphorus efficiency（System）, while overcoming conventional hybridization breeding side
Phenomena such as Phenotypic Selection difficulty and Linkage drag that method is brought, increase substantially phosphorus efficiency kind（System）Breeding efficiency.This
Technical method is equally applicable to the genetic improvement of other crop phosphorus efficiencies.
Table 1
Table 1 is used for 21 couples of SSR of soybean phosphorus efficiency screening（Simple sequence repeat, simple sequence repeats）Primer
Sequence.
Description of the drawings
Fig. 1 soybean phosphorus efficiency related root traits recombinant inbred lines（RILs）Build flow；
Fig. 2 soybean phosphorus efficiency QTLs positioning analysis flow charts；
The soybean hybridization separation progeny population of Fig. 3 primers satt519 amplifications, wherein No. 1-4 is wild species, and No. 5-10 is 6
RIL, No. 35 are BD2, and No. 92 are BX10, remaining is soybean hybridization separation progeny population；
Amplification of Fig. 4 phosphorus efficiencies specificity SSR primers in soybean hybridizes separation progeny population；
Fig. 5 soybean phosphorus efficiency near isogenic lines（NILs）The flow chart of structure；
Fig. 6 shifts the breeding procedures figure of phosphorus efficiency QTLs using phosphorus efficiency NILs quick hybridizations.
Fig. 7 pcr amplification reaction program parameter figures
Specific implementation mode
Phosphorus efficiency kind is cultivated using soybean root dry mass near isogenic lines（System）Method, follow the steps below：
（1）Build phosphorus efficiency related root traits recombinant inbred lines：The Boluo County local varieties of inefficient with phosphorus, deep root type
BD2 is female parent, and phosphorus efficiency, shallow root type, Brazil introduce commercial variety BX10 as male parent, and hybridization generates F1Generation, selfing obtain F2Generation,
Through single seed descent to F9Generation（Or F9It is more than generation）, obtain F9For recombinant inbred lines RIL (F9)。
（2）Phosphorus efficiency QTLs positioning：With above-mentioned F9 for recombinant inbred lines RIL (F9) it is material, respectively in high phosphorus（160kg/
hm2）, it is low-phosphorous（Do not apply phosphorus）It is planted under the conditions of two kinds, phosphorus efficiency QTL positioning analysis is carried out using SSR marker（Detailed process is shown in figure
2）, 7 sites of acquisition and the low-phosphorous condition efficient absorption of soybean utilize related in B1, C1, D1a, D2 and F linkage group（Liang Quan etc.,
The Genetic Analysis of soybean root system character under different P levels, Molecular Plant Breeding, 2009,7：321-329；Liang et
al., QTL analysis of root traits as related to phosphorus efficiency in
Soybean, Annals of Botany, 2010,106: 223–234）.Inventor's section at left and right sides of above-mentioned 7 sites adds
Secret mark is remembered, has selected 100 pairs of SSR primers altogether, and expanded using 94 Soybean Germplasms, has finally obtained 21 pairs and phosphorus
The SSR primers that efficient target QTLs is closely chain, amplification polymorphism is good（Fig. 3, Fig. 4）, the special SSR marker of this 21 couple is Sat_
128、Sat_149、Satt519、Satt298、Sat_348、B1-5、B1-11、B1-18、Sat_296、Sct_192、Satt458、
Satt135, Sat_277, D2-2, D2-9, Sat_343, Satt507, Sct_010, Satt076, Sat404, Satt042, sequence
It is listed in table 1.
（3）Phosphorus efficiency near isogenic lines obtains：It is female parent with BX10, BD2 is that male parent carries out hybridization acquisition F1 generation hybrid, with
BD2 is recurrent parent, adds generation for backcross method and strange land using height, with the relevant 21 pairs of SSR markers combination phenotype of above-mentioned phosphorus efficiency
Backcross progeny individual is screened, through 5 backcrossings and molecular marker assisted selection, the final near isogenic lines for obtaining the QTLs containing target,
These phosphorus efficiency near isogenic lines are shown and phosphorus efficiency from plant growing way, root traits, biomass, phosphorus content etc.
The similar characteristic features of shallow root type donor parents BX10.
（4）The method that phosphorus efficiency genotype is screened from backcross progeny segregating population, includes the following steps：1. setting control
Group sets up the phosphorus efficiency having experimentally confirmed that, phosphorus inefficient cultivar for control, with BX10（Brazil 10）, 3 F10Generation recombinates is certainly
RIL71, RIL97, RIL130 are phosphorus efficiency kind, BD2（It is No. 2 local）, 4 wild soybean YN01, YN02, YN03, YN04,3
A F10 can also increase and decrease check variety for recombinant inbred lines RIL32, RIL144, RIL171 phosphorus inefficient cultivar according to actual conditions
Quantity；2. extracting soybean leaves total DNA using CTAB methods or commercially available reagent box；3. select following SSR molecular marker Sat_128,
Sat_149、Satt519、Satt298、Sat_348、B1-5、B1-11、B1-18、Sat_296、Sct_192、Satt458、
Satt135, Sat_277, D2-2, D2-9, Sat_343, Satt507, Sct_010, Satt076, Sat404, Satt042, and close
At primer, synthesized primer is diluted to 50 μM according to OD values and molecular size range, by primer, is placed in -20 DEG C of refrigerators and preserves；④
PCR amplification specific section, using the PCR reaction systems of 20 μ l, composition is as follows：
10×PCR Buffer（Containing Mg2+） 2.0 µl
dNTP（10 mmol/L） 0.3 µl
Upstream and downstream primer（50µmol/L）Each 0.06 μ l
Taq enzyme（5U/µl） 0.2 µl
1 μ l of template DNA（About 20 ng）
Distilled water is mended to 20 μ l
It after mixing, is placed in PCR instrument and is expanded, the parameter that amplified reaction program is finally selected is shown in Fig. 7.
According to the different Tm values of each pair of primer, the appropriate annealing temperature adjusted in response procedures is general to can be obtained with 52 DEG C
Preferable amplification；5. 7.2% denaturing polyacrylamide gel electrophoresis of pcr amplification product（PAGE）Detection, 0.5 × TBE are slow
The loading indicator of 5 μ L is added in fliud flushing in PCR product per hole, and with microsyringe 2.0 μ l samples are taken extremely after vibrating mixing
In loading wells, 80 mA of electrophoresis tank constant current, according to primer size, general dimethylbenzene cyanines can stop when being displaced downwardly to the 2/3 of gel,
Electrophoresis electrophoresis terminates, and taking-up gel is dyed with after rinsing, is read tape and records banding pattern data；6. each pair of SSR primer detections 1
A site regards every polymorphism band as 1 allele, wherein there is polymorphism band to be denoted as（1）With being denoted as nothing（0）, according to expansion
Increase band and builds SSR marker data matrix；7. calculating genetic similarity using NTsys 2.10e softwares and structure UPGMA losing
Pass dendrogram.8. according to cluster result, with reference to control group as a result, can determine whether phosphorus efficiency soybean genotype.
（5）With Zheng 9525（State examines beans 2009029）For receptor parent（Commercial variety may be used in receptor parent, can also adopt
Use local varieties）, the one of strain A3 of phosphorus efficiency near isogenic lines for choosing above-mentioned acquisition is donor parents, carries out hybridization and turns
Move phosphorus efficiency target QTL.Genome-wide screening is carried out to hybridization separation offspring using above-mentioned 21 pairs of SSR markers, specific steps are shown in
Embodiment 2.It, can Rapid identification phosphorus efficiency filial generation material in conjunction with Phenotypic Selection.Detailed process is shown in Fig. 6.
The present invention builds phosphorus efficiency RILs and phosphorus efficiency NILs by priority, close using phosphorus efficiency QTLs positioning and phosphorus efficiency
Chain specific molecular marker assisted Selection technology is cut, without relying on the gene cloning and transgenosis skill that time-consuming, cost is high
Art, while phenomena such as overcome difficult Phenotypic Selection that conventional cross-breeding brings and Linkage drag, realize phosphorus nutrition efficiently,
The quick integration of high yield, Fineness gene, efficiently and accurately obtains that root dry mass is excellent, soybean varieties of phosphorus efficiency（Strain）, substantially
Degree improves phosphorus efficiency kind（System）Breeding efficiency.This technology method is equally applicable to the genetic improvement of other crop phosphorus efficiencies.
Claims (1)
1. cultivating phosphorus efficiency kind using soybean root dry mass near isogenic lines（System）Method, it is characterised in that：According to the following steps
It carries out：
（1）Using two geographical distant races and have confirmed the soybean varieties that there is significant difference in root dry mass, phosphorus efficiency etc.
Hybridization builds the recombinant inbred lines of root traits correlation phosphorus efficiency（RILs）, and apply simple sequence repeats（SSR）Label is just fixed
Position soybean phosphorus efficiency target QTLs；
（2）In above-mentioned phosphorus efficiency target QTLs both sides encrypted indicias and polymorphism screening is carried out, it is final to obtain and phosphorus efficiency target
QTLs is closely chain, has the specific SSR marker of polymorphism；
（3）With step（1）In soybean phosphorus efficiency genotype be donor parents, using the kind of the inefficient product of phosphorus as recurrent parent, profit
Use step（2）In specific SSR marker screen and hybrid generation selected in conjunction with phenotype, through 4~6 backcrossings and
Molecular marker assisted selection, obtains the target QTLs of phosphorus efficiency containing soybean and genetic background phosphorus similar with recurrent parent height is high
Imitate root dry mass near isogenic lines（NILs）；
（4）With above-mentioned soybean phosphorus efficiency NILs for parent or intermediate materials, using domestic place or commercial variety as receptor parent into
Row sexual hybridization, by step（2）To hybridization, separation offspring carries out genome-wide screening and M8003 line, obtain root dry mass it is excellent,
The good new soybean varieties of phosphorus efficiency, Comprehensive Traits（System）.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111652324A (en) * | 2019-10-12 | 2020-09-11 | 沈阳农业大学 | Rapid identification method for genotype soybean varieties with different phosphorus efficiencies |
| CN115644055A (en) * | 2022-10-14 | 2023-01-31 | 吉林省农业科学院 | Low-phosphorus-resistant breeding and culturing method for soybean |
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| CN107058496A (en) * | 2017-01-20 | 2017-08-18 | 云南农业大学 | A kind of SSR primer sequences screened suitable for soybean phosphorus efficiency and its application method |
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2017
- 2017-12-29 CN CN201711470689.4A patent/CN108308018A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107058496A (en) * | 2017-01-20 | 2017-08-18 | 云南农业大学 | A kind of SSR primer sequences screened suitable for soybean phosphorus efficiency and its application method |
Non-Patent Citations (2)
| Title |
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| LIANG ET AL.: "QTL analysis of root traits as related to phosphorus efficiency in soybean", 《ANNALS OF BOTANY》 * |
| 梁泉等: "不同磷水平下大豆根系性状的遗传特性分析", 《分子植物育种》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111652324A (en) * | 2019-10-12 | 2020-09-11 | 沈阳农业大学 | Rapid identification method for genotype soybean varieties with different phosphorus efficiencies |
| CN111652324B (en) * | 2019-10-12 | 2023-10-03 | 沈阳农业大学 | Rapid identification method for soybean varieties with different phosphorus efficiency genotypes |
| CN115644055A (en) * | 2022-10-14 | 2023-01-31 | 吉林省农业科学院 | Low-phosphorus-resistant breeding and culturing method for soybean |
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