CN100441229C - Sterilizing and disinfection agent - Google Patents
Sterilizing and disinfection agent Download PDFInfo
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- CN100441229C CN100441229C CNB2006100056125A CN200610005612A CN100441229C CN 100441229 C CN100441229 C CN 100441229C CN B2006100056125 A CNB2006100056125 A CN B2006100056125A CN 200610005612 A CN200610005612 A CN 200610005612A CN 100441229 C CN100441229 C CN 100441229C
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Abstract
The present invention provides a novel sterilizing agent of a hydrogen peroxide composition. The composition comprises the constituents and the mass concentration (g/100mL): 0.1 to 30 of hydrogen peroxide, 0.05 to 15 of aromatic carboxylic acid, 0.05 to 15 of inorganic acidic substances, 0 to 10 of corrosion inhibitors, 1 to 70 of hydrophilic solvent and water as the rest. The composition sterilizing agent has a strong spore killing function and can be used for sterilizing medical appliances of operation, dental appliances and endoscopes. Medical appliances soaked in the sterilizing agent to be sterilized do not need water washing except for endoscopes to be flushed by sterile water or sterilizing water, and other medical appliances are dried up by sterile gauze. The sterilizing agent is mainly characterized in that the sterilizing effect of the sterilizing agent can not be weakened within a certain storage period but can be enhanced greatly. The sterilizing agent can be used for sterilizing foodstuff processing utensils, foodstuff packaging material and containers and can also be used for sterilizing environments and places of public places, catering industry, graziery, etc. or used for sterilizing physical surfaces.
Description
Technical field
The present invention relates to a kind of disinfection sanitizer, relate in particular to the application of hydrogen peroxide composition disinfectant in the sterilization of medical instrument technology.
Background technology
First-selected pressure-steam sterilization method is now generally acknowledged in the sterilization of medical apparatus and instruments.But, suit to sterilize with chemosterilant to those medical apparatus and instruments of making by heat-sensitive material.Peracetic acid, chlorine-containing disinfectant have bactericidal effect preferably, but because of it has the strong impulse abnormal smells from the patient, the aqueous solution instability to shortcomings such as metal are corrosive, should not be used for the sterilization of medical apparatus and instruments.Oxirane can be used for the sterilization of medical apparatus and instruments, but its gas is inflammable and explosive, and safety in utilization is poor, and needs the certain device condition, uses and is subjected to certain limitation.Glutaraldehyde is a kind of biocide, is generally used in the sterilization of medical instrument technology.But according to the literature, expose repeatedly that some can cause headache and anaphylaxis (Xue Guangbo, practical Disinfection, 1986 behind the glutaraldehyde to the open air; 267-277); Animal experiment shows that the glutaraldehyde 1mL with 2% is expelled in the experimental animal muscle, can cause the part and inflammatory reaction or larger area tissue necrosis (Li Faliang, Chinese Disinfection magazine, 2003 occur; 20 (3): 190-191).The glutaraldehyde alkali condition is down unstable, easily produces polymerization and loses bactericidal activity; More stable under the acid condition, but bactericidal effect is poor.This shows: the glutaraldehyde disinfectant that uses as routine still has its defective, is not very satisfactory.
Hydrogen peroxide is a kind of efficient, safe disinfectant, and it is simple in structure, and catabolite is water and oxygen, and environment is not produced the residual toxicity effect.Hydrogen peroxide has been widely used in the food industry such as bleaching of the sterilization of packaging material for food, food containers and raw-food material as antiseptic sterilization agent, bleach; Wound is handled with its concentration solution of 3% at medical field, made gargarism adjuvant drug (Ministry of Public Health, disinfection technology standard, version in 2002: 164) as stomatitis, tonsillitis, pharyngolaryngitis with 1-1.5% concentration solution.But hydrogen peroxide low concentration poor stability, required bacteriocidal concentration height, long action time makes its application be subjected to certain restriction.
Hydrogen peroxide is that catabolite does not produce secondary pollution to external environment as the outstanding advantage of disinfectant, the requirement of the protection that conforms.For making full use of this advantage, this area research worker in hydrogenperoxide steam generator by certain match well add have potentiation, one or more components of stable and antiseptic property, be mixed with the hydrogen peroxide composition disinfectant, desire to make it to reach efficiently, stablize, hang down corrosive idealized disinfectant, so that be applied in the larger context.CN97114457.5 and CN03117945.2 have carried out the research report to hydrogen peroxide composition, observe but only it has been carried out research as detergent or environment and daily living article disinfectant, do not relate to the application of hydrogen peroxide composition disinfectant in the medical apparatus sterilizing sterilization technology.
Seek a kind of hydrogen peroxide composition disinfectant, make that it reaches efficiently, safe, stable, nontoxic, low corrosive performance; And be applied to go in medical apparatus and instruments or the wider field of disinfection and sterilization, urgent requirement is arranged.Primary and foremost purpose of the present invention expects to satisfy this requirement exactly.
Summary of the invention
The invention provides a kind of hydrogen peroxide composition disinfectant, its compositions comprises following component and mass concentration: hydrogen peroxide 4.64-4.80g/100mL, aromatic carboxylic acid 0.05-15g/100mL, mineral acid material 0.05-15g/100mL, the organic inhibitor 0-8g/100mL compatible with hydrogen peroxide, hydrophilic solvent 1-70g/100mL, surplus is a water.
Hydrogen peroxide is that active component plays sterilization in the present composition.
Described component aromatic carboxylic acid is a benzoic acid, mainly plays stable or Synergistic biocidal effect; The preferred 0.1-8g/100mL of its mass concentration.
Described component mineral acid material mainly plays stable and acidity adjustment effect, regulates disinfectant pH value 1-5 with it, selects inorganic phosphate or Metaphosphoric acid for use; The preferred 0.1-8g/100mL of its mass concentration.
The compatible organic inhibitor of described component and hydrogen peroxide mainly plays the corrosion-resisting function to metal material.
Described component hydrophilic solvent act as the material that is insoluble in water in the dissolved constituent and the Synergistic biocidal effect is provided.Select a kind of or its two kinds the mixture in ethanol and the isopropyl alcohol for use; The preferred 2-60g/100mL of its mass concentration.
The pH value of the various prescriptions of hydrogen peroxide composition disinfectant of the present invention is 1-5.
Present composition disinfectant preparation method is easy.The steps include: earlier aromatic carboxylic acid to be dissolved in the hydrophilic solvent, add a certain amount of pure water after, add corrosion inhibiter and liquid hydrogen peroxide, treat dissolving fully after, regulate pH value 1-5 with the mineral acid material, the reuse pure water is added to required total amount.After placing ambient temperature overnight, use the titration of potassium permanganate content of hydrogen peroxide, it is qualified that concentration relative error≤10% of preparing as the content and the desire of its mensuration o'clock is.According to the different application purpose, can prepare the hydrogen peroxide composition disinfectant of variable concentrations; Also can prepare the compositions of higher concentration earlier, water or hydrophilic solvent are done suitably dilution during use.With this compositions disinfectant of higher concentration of preparation, after depositing 2-4 month under 25 ℃ of-50 ℃ of conditions, can improve its sterilizing ability, obtain the better application effect.
Described hydrogen peroxide composition antiseptic sterilization agent, its general guiding using method is as follows:
1. to the sterilization of general article surface, food processing apparatus, packaging material for food and container, be after this compositions disinfectant of 46.0g/L adds water and does dilution in 1: 10, to spray or wiping action 5-10 minute with the hydrogen peroxide mass concentration.
2. the sterilization of medical apparatus and instruments and articles for use and sterilization.To the general curative apparatus that only needs to do to disinfect and articles for use the scope etc. of human body natural's passage and tube chamber mucosa (as enter), be this compositions disinfectant action of soaking 10-30 minute of 46.0g/L with the hydrogen peroxide mass concentration.To operation medical apparatus and instruments and articles for use, dental instrument and enter human body sterilization tissue, organ or enter the scope etc. of aseptic chamber through surgical incision, need reach medical apparatus and instruments and articles for use (as heat labile operation articles for use, peritoneoscope, cystoscope, hysteroscope etc.) that sterilization requires, be this compositions of 46.0g/L disinfectant action of soaking 3 hours with the hydrogen peroxide mass concentration.
Other component in hydrogen peroxide and the compositions all is materials nontoxic or that toxicity is very low, and low concentration does not all have obvious damage to article and tissue.Therefore, with medical apparatus and instruments and the articles for use after this compositions disinfectant soaking disinfection sterilization treatment, except that scope needs to do the flushing with sterilized water or disinfectant, other medical apparatus and instruments all need not used aseptic water washing, just dries and can use with sterile gauze.
3. what medical instrument cleaning was preceding disinfecting.Is after this compositions of 46.0g/L disinfectant is soaked 10-20 minute to used medical apparatus and instruments with the hydrogen peroxide mass concentration, washs, washes with flowing water, drains or dries, and gets final product.
Hydrogen peroxide composition disinfectant bactericidal assay of the present invention and correlated performance test experience result:
1. bactericidal assay:
1.1 suspension method kill vegetative forms of bacteria test:
The fresh Nutrient agar Dou face culture of depletion Staphylococcus aureus (ATCC6538), escherichia coli (ATCC8099) is made bacteria suspension with 0.1% tryptone normal saline (TPS).The hydrogen peroxide composition disinfectant 5.0mL that adds variable concentrations is in test tube, put 20 ℃ of water-bath preheating 10min, add above-mentioned bacteria suspension 0.1mL, affact the scheduled time, taking-up 0.5mL adding contains the 4.5mL nertralizer (to be identified through neutralization test: in the test tube PBS that selects 0.5% sodium thiosulfate, 3% tween 80,0.3% lecithin for use), act on 10min.Get stock solution or its diluent 1.0mL and do the viable bacteria counting, cultivate the 2d observed result for 37 ℃.See Table 1
Table 1 present composition disinfectant kill vegetative forms of bacteria effect
Annotate: the result is 3 test meansigma methodss, and test temperature is 20 ℃.Positive control on average reclaims the bacterium amount: staphylococcus aureus 5.3 * 10
6Cfu/mL, escherichia coli 3.8 * 10
6Cfu/mL
Conclusion: with the hydrogen peroxide mass concentration is the compositions disinfectant of 4.58g/L, all can make it kill logarithm value (KL)＞5.00 to staphylococcus aureus, escherichia coli effect 5min, reaches the disinfection standard requirement.
1.2 support methods kill bacteria spore test:
Following carrier qualitative is quantitatively killed the spore test and is all undertaken by " disinfection technology standard " (version in 2002) described method and step.
Bacillus subtilis var (ATCC9372) spore is prepared liquid, and (TSB) is diluted to 1 * 10 with pancreas peptone soybean broth
8-5 * 10
8The bacteria suspension of cfu/mL drips and dyes on the stainless steel substrates of 10mm * 10mm, makes its bacteria containing amount reach 5 * 10
5-5 * 10
6The cfu/ sheet, 37 ℃ of drying for standby.By the amount of every truffles sheet 5.0mL, add the hydrogen peroxide composition disinfectant in aseptic plate, put preheating 10min in 20 ℃ of water-baths, add the microbiological contamination sheet, take out the bacterium sheet after affacting the scheduled time.When doing the carrier quantitation test, bacterium sheet input is added with the 5.0mL nertralizer (to be identified through neutralization test: act on 10min in the test tube PBS that selects 0.2% sodium thiosulfate, 0.3% lecithin, 3% tween 80 for use), get stock solution or its diluent 1.0mL and do the viable bacteria counting, cultivate the 3d observed result for 37 ℃.When doing the carrier qualitative test, the bacterium sheet is dropped into 5.0mL contain in the TSB meat soup of above-mentioned nertralizer, cultivate the 7d observed result for 37 ℃.Simultaneously, kill spore effect contrast test with the simple hydrogenperoxide steam generator conduct of same concentrations.The results are shown in Table 2,3.
Table 2 present composition disinfectant and simple hydrogenperoxide steam generator carrier quantitation kill the spore effect
Annotate: experiment repeats 5 times, and test temperature is 20 ℃.Denominator is test a total of tubes, the positive pipe number of molecule in the table.Positive control on average reclaims bacterium amount 2.6 * 10
6The cfu/ sheet.
Conclusion: with the hydrogen peroxide mass concentration is this compositions disinfectant effect 90min of 47.6g/L, can kill the bacillus subtilis black variety gemma that dyes on stainless steel substrates fully, reaches the sterilization standard-required; And the simple hydrogenperoxide steam generator effect 120min of same concentrations still has bacteria growing, can not reach the sterilization requirement.
2. medical apparatus and instruments simulated field sterile test:
The spore suspension of (1.2) preparation is dripped and dyes on the increment of the mosquito forceps that has blocked, make its bacteria containing amount reach 10
6The cuf/ handle, 37 ℃ of drying for standby.By every the amount of microbiological contamination mosquito forceps 10mL, the compositions disinfectant that adds concentration of hydrogen peroxide and be 47.6g/L is put in 20 ℃ of water-baths and is incubated 10min in aseptic plate.20 microbiological contamination mosquito forcepss are soaked in act on 120min in the disinfectant solution, take out be put in respectively 10mL contain nertralizer (identifies through neutralization test: in the TSB meat soup PBS that selects 0.6% sodium thiosulfate, 0.3% lecithin, 3% tween 80 for use), 37 ℃ of cultivation 7d observed results.Simultaneously, do negative and the positive control test.
The result: test repeats 3 times.Test temperature is 20 ℃.60 are dyed the mosquito forceps that bacillus subtilis black variety gemma is arranged, and are equal asepsis growth behind the 47.6g/L compositions disinfectant effect 120min with the hydrogen peroxide mass concentration, reach the sterilization standard-required.The negative control asepsis growth, positive control is cultivated 24h bacteria growing, and positive control on average reclaims the bacterium amount and is: 1.8 * 10
6The cfu/ handle.
3. stability in use test continuously:
With 2000mL hydrogen peroxide mass concentration is that the compositions disinfectant solution of 47.6g/L is poured in the enamel square tray with cover, puts into surgical medical apparatuses such as mosquito forceps, needle forceps, tweezers, makes it reach fully loaded requirement.Take out apparatus and wash, drain, put into disinfectant solution with water every day.After two weeks, the cancellation venom is done bactericidal assay by (2) described method and step so repeatedly.Test temperature is 20 ℃.
The result: test repeats 3 times.The medicining liquid dipping effect 120min of above-mentioned continuous service test after two weeks can kill the bacillus subtilis black variety gemma that dyes on mosquito forceps fully, with comparison indifference before the continuous service test.Positive control on average reclaims the bacterium number: 1.9 * 10
6The cfu/ handle
4. stability test:
Find in experimentation: this compositions disinfectant is after accelerated test and natural room temperature are deposited certain hour, and its bactericidal effect does not see that not only reduction strengthens on the contrary unexpectedly to some extent.For confirming its reliability, the disinfectant after depositing has been carried out the comparatively coherent detection of system.Now be summarized as follows respectively:
Below Xiang Guan bactericidal assay is all carried out according to " disinfection technology standard " (version in 2002) described method and step.The nertralizer of used variable concentrations is all cooked neutralization test once more with the disinfectant after depositing and is identified.The result: former nertralizer with variable concentrations can effectively neutralize and deposit used disinfectant in each corresponding bactericidal assay of back.
4.1 the disinfectant of this compositions after depositing carrier qualitative quantitative sterilization effect:
Test organisms is bacillus subtilis var (ATCC9372) spore.Method and step by (1.2) are carried out bactericidal assay.The PBS compound recipe nertralizer of 0.2% sodium thiosulfate, 3% tween 80,0.3% lecithin is adopted in the carrier quantitation test.The carrier qualitative test TSB meat soup that contains above-mentioned nertralizer.The results are shown in Table 4,5
This compositions disinfectant carrier quantitation bactericidal effect after table 4 is deposited
Annotate: above every test repeats 5 times.Test temperature is 20 ℃.
This compositions disinfectant carrier qualitative bactericidal effect after table 5 is deposited
Annotate: above-mentioned every test repeats 5 times.Test temperature is 20 ℃.Denominator is test a total of tubes, the positive pipe number of molecule in the table.
4.2 the disinfectant of this compositions after depositing is to medical apparatus and instruments simulated field sterile test:
With the hydrogen peroxide mass concentration be 47.6g/L this compositions disinfectant in 37 ℃ deposit 90d after, carry out medical apparatus and instruments simulated field sterile test by (2) described method and step.Test repeats 3 times, as a result 60 dye bacillus subtilis black variety gemma is arranged mosquito forceps with above-mentioned disinfectant action of soaking 30min after equal asepsis growth, reach the sterilization standard-required.Positive control on average reclaims the bacterium number: 2.3 * 10
6The cfu/ handle
4.3 the continuous stability in use test of the disinfectant of this compositions after depositing:
With concentration is to take out after 37 ℃ of this compositions disinfectant of 46.4g/L are deposited 90d, carries out continuous stability in use test by (3) described method and step.Test organisms is bacillus subtilis var (ATCC9372) spore.The bactericidal assay temperature is 20 ℃.The results are shown in Table 5
The continuous stability in use result of the test of this compositions disinfectant after table 5 is deposited
Annotate: denominator is test a total of tubes, the positive pipe number of molecule, the mosquito forceps of medical apparatus and instruments simulation test carrier for having blocked in the table.Because the continuous volatilization of alcohols material is concentrated effective ingredient in the disinfectant, along with its content of hydrogen peroxide of prolongation of life cycle slightly increases.
Conclusion: the content of hydrogen peroxide measurement result shows: this compositions disinfectant is along with the prolongation of storage period, and the content of main component hydrogen peroxide descends gradually to some extent.Natural room temperature (15 ℃-38 ℃) is deposited 120 days, 54 ℃ 14 days, the 37 ℃ average rate of decrease after 90 days and is respectively 3.45%, 7.56% and 13.42%.Yet the bactericidal effect after this compositions disinfectant is deposited is not because of the reduction of content of hydrogen peroxide weakens, and on the contrary, its bactericidal effect has enhancing greatly.Through experiment confirm repeatedly, the disinfectant before depositing is killed the bacillus subtilis black variety gemma that dyes on stainless steel carrier fully needs action of soaking 90min; 120 days, 54 ℃ 14 days, the 37 ℃ disinfectant after 90 days only need effect 20min, 10min and 15min just can reach same effect respectively and deposit room temperature.The fluctuation of disinfectant pH value is little before and after depositing after testing.We think and may exist a kind of chemical reaction slowly in the present composition system, have produced a kind of have potentiation or synergistic material.
This compositions disinfectant is deposited 37 ℃ and is done continuous service test again after 90 days, and its bactericidal effect of back is not seen yet and weakened all around.Because of the continuous volatilization of alcohols material in the disinfectant solution, its concentration of hydrogen peroxide is slightly increased along with the prolongation of service time.In view of the above, we think that this compositions disinfectant can use for three weeks at least continuously, too much only need add a small amount of new disinfectant and get final product as alcohols material volatilization in actual use.
5. Organic substance interference test:
Test organisms is bacillus subtilis var (ATCC9372) spore, is the Organic substance representative with the calf serum.The diluent that contains 25% and 50% aseptic calf serum with the TPS preparation prepares the spore suspension respectively and makes the microbiological contamination sheet.The bacterium sheet made from the TPS that does not contain calf serum carries out carrier quantitative germicidal test with this compositions disinfectant of the mass concentration 46.4g/L of new preparation in contrast.Operating procedure is seen (1.2).The results are shown in Table 6
Table 6 Organic substance interference test
Annotate: test repeats 3 times.Test temperature is 20 ℃.
Conclusion: Organic substance does not have influence substantially to the bactericidal effect of present composition disinfectant.
6. clinical result of use checking:
This compositions disinfectant that with concentration is 46.4g/L is carried out the result of use checking to dental clinic.Its using method is: exhausted dental instruments with the clear water washing, drains behind preliminarily pasteurized, and this compositions of reuse disinfectant is soaked in side's enamel tray with cover spends the night.Time spent removal equipment (not needing aseptic water washing) is dried the back with sterile gauze and is directly used.After using for two weeks so continuously, the stainless steel instruments of immersion does not have iron mold, and mouth mirror minute surface light as before.Take out disinfectant solution, surveying its content of hydrogen peroxide is 45.4g/L, comparison indifference before killing action time of dying the bacillus subtilis black variety gemma on carrier fully and using.Wipe away to dip in cotton and get this compositions disinfectant, be applied on volunteer's the oral mucosa, gingiva after the disinfection, check the mucosa not damaged,, do not have uncomfortable reaction except that feeling slightly the tart flavour.
7. corrosion test:
With concentration of hydrogen peroxide is this compositions disinfectant of 47.6g/L, carries out corrosion test by " disinfection technology standard " (version in 2002) described method and step.Experimental result is judged by the corrosivity grade scale: this compositions disinfectant of this concentration does not have corrosion substantially to rustless steel, and carbon steel is had mild corrosion, and copper and aluminum are all had the moderate corrosion.The rustless steel operating theater instruments was soaked in during the stability in use test continuously in the disinfectant accumulative total nearly 80 days, but do not see that yet apparatus has rust staining, as excellent as before.In addition, an illuminator is soaked in surplus this compositions disinfectant 150 of above-mentioned concentration day, it is clear, smooth to observe minute surface, does not have the erosion trace.
Advantage of the present invention:
Hydrogen peroxide composition disinfectant of the present invention compares with simple hydrogen peroxide, has advantages such as efficient, stable, that corrosivity is low.The compositions disinfectant that hydrogen peroxide mass concentration of the present invention is 47.6g/L, effect 90min can kill the bacillus subtilis black variety gemma that dyes on stainless steel carrier fully, reaches the sterilization standard-required; And the simple hydrogenperoxide steam generator of same concentrations acts on 120min and still can not reach the sterilization requirement under the situation of bactericidal assay term harmonization.The chemical assay stability test is the result show: present composition disinfectant stable phase can reach 1 year at least, and deposit room temperature 120 days, 54 ℃ 14 days and 37 ℃ are after 90 days, and the effective ingredient average rate of decrease is respectively 3.45%, 7.56% and 13.42%; And natural room temperature is deposited in the simple disinfectant with hydrogen peroxide agent of 10% concentration 3 months, 54 ℃ 14 days and 37 ℃ are after 90 days, and the effective ingredient average rate of decrease is respectively 7.05%, 37.43% and 38.53% (Sun Shouhong, Chinese Disinfection magazine, 2003; 20 (1): 6-9).The microbiological assay stability test is the result show: present composition disinfectant is deposited 37 ℃ of its bactericidal effects after 90 days and is not only weakened on the contrary and strengthen to some extent (decidable effective storage period be 2 years).Present composition disinfectant does not have corrosion substantially to rustless steel, and carbon steel is only had mild corrosion, obviously is better than simple hydrogenperoxide steam generator.
Present composition disinfectant is used for the sterilization of medical apparatus and instruments and the glutaraldehyde disinfectant of 20g/L relatively has outstanding superiority.(1), lack germicidal efficiency height, action time.Hydrogen peroxide mass concentration of the present invention is 47.6g/L compositions disinfectant effect 90min, can kill the bacillus subtilis black variety gemma that dyes on stainless steel carrier fully, reaches the sterilization standard-required; And reaching equal effect, the glutaraldehyde disinfectant of 20g/L concentration need act on 240min (Huang Yuhong, Chinese Disinfection magazine, 2004; 21 (3): 227-228) (2), present composition disinfectant can directly be used, and its disinfectant uses the liquor ratio glutaraldehyde disinfectant to have better stability.Concentration is that this compositions of 46.4g/L disinfectant was deposited room temperature after 120 days, and it kills the action time of dying the bacillus subtilis black variety gemma on stainless steel carrier fully, shortens to 20min by the 90min before depositing.20g/L compound glutaraldehyde disinfectant uses liquid to deposit room temperature after 70 days, and extend to 5h (Chen Yueying etc., Chinese Disinfection magazine, 1999 by original 3h the action time that suspension method is killed bacillus subtilis black variety gemma; 16 (1): 11-14).(3), the use of present composition disinfectant is more safer, easy than glutaraldehyde disinfectant.Glutaraldehyde disinfectant has certain toxicity, and tissue is had solidification, and idol has untoward reaction such as sensitization.In actual applications, have because of the medical apparatus and instruments flushing of soaking not thoroughly, remain in report (Xu Li equality, Chinese Disinfection magazine, 2005 that the glutaraldehyde on the apparatus causes the patient anaphylaxis to take place even anaphylactic shock takes place with glutaraldehyde; 22 (1): 96-98).Therefore, the medical apparatus and instruments that soaks after sterilizing with glutaraldehyde disinfectant must wash with sterilized water repeatedly, thoroughly removes the glutaraldehyde disinfectant that remains on the apparatus, could use after the reuse sterile gauze is dried.Hydrogen peroxide is nontoxic substantially, no sensitization, to the tissue no solidification, low concentration is little to the skin mucosa zest.In actual applications, typical concentrations is the hydrogen peroxide treatment wound of 30g/L, is that the hydrogen peroxide of 15g/L is made gargarism etc. with concentration.This compositions disinfectant main component is a hydrogen peroxide, and the disinfectant solution hydrogen peroxide mass concentration of using that is used for sterilization treatment only is 47.6g/L.Therefore, use this compositions disinfectant medical apparatus and instruments is carried out the soaking disinfection sterilization, the apparatus behind its sterilization need not to use aseptic water washing, takes out the back and just dries and can directly use with sterile gauze.(relevant this point is confirmed in the practical application of dental instruments sterilization treatment.) (4), present composition disinfectant does not produce secondary pollution to environment, the requirement of the protection that more conforms.The main component of this compositions is a hydrogen peroxide, and the catabolite of hydrogen peroxide is water and oxygen, can not pollute external environment.Therefore, use disinfectant later outwards to discharge and have better safety.
The outstanding advantage of this compositions disinfectant is that its bactericidal effect does not only weaken in certain storage period, has on the contrary greatly to strengthen.Repeated multiple times ground carrier qualitative and carrier quantitation result of the test show: this compositions disinfectant is deposited 120 days, 54 ℃ 14 days and 37 ℃ after 90 days in room temperature, it kills the action time of dying the bacillus subtilis black variety gemma on stainless steel carrier fully, shortens to 20min, 10min and 15min respectively by the 90min before depositing.Medical apparatus and instruments simulated field sterile test and continuous stability in use result of the test also show: this compositions disinfectant is killed the action time of dying the bacillus subtilis black variety gemma on mosquito forceps fully, and being shortened to by original 120min only needs 30min.Do continuous stability in use test again with depositing 37 ℃ of this compositions disinfectant after 90 days, continuously around the service test after, indifference relatively before its bactericidal effect and the test.At present, the disinfectant products sales section is longer demurrage, and the effective ingredient of most monobasic packaging products all descends gradually to some extent in storage, transportation and sales process, causes weakening of its actual bactericidal effect.In order to ensure the bactericidal effect of disinfectant products, the counter-measure one of manufacturer is to strengthen the concentration of disinfectant effective ingredient, makes in its concentration range that product active constituent content still can guarantee to indicate at product in long sales cycle; The 2nd, strict control volume of production reduces product inventory as much as possible, shortens the demurrage of product in sales section to greatest extent.The present invention can solve the concrete above-mentioned practical problem that manufacturing enterprise is run into of implementing preferably in process of producing product, significant to bactericidal effect and the safety in actual use of guaranteeing its product.
The specific embodiment
The present invention does to set forth further by following examples, but does not limit the scope of the invention.
Embodiment 1: hydrogen peroxide 48g, and benzoic acid 5g, isopropyl alcohol 450g, corrosion inhibiter 4g adds an amount of pure water dissolving, transfers pH value 1-5 with phosphoric acid, adds pure water to 1000mL.
Embodiment 2: hydrogen peroxide 48g, and benzoic acid 10g, ethanol 350g, corrosion inhibiter 6g adds an amount of pure water dissolving, transfers pH value 1-5 with phosphoric acid, adds pure water to 1000mL.
Embodiment 3: hydrogen peroxide 48g, and benzoic acid 4g, ethanol 250g, isopropyl alcohol 300g, corrosion inhibiter 10g adds an amount of pure water dissolving, transfers pH value 1-5 with phosphoric acid, adds pure water to 1000mL.
Embodiment 4: hydrogen peroxide 48g, and benzoic acid 5g, ethanol 400g, corrosion inhibiter 5g adds an amount of pure water dissolving, transfers pH value 1-5 with phosphoric acid, adds pure water to 1000mL.
Experiment confirm: the prescription action of soaking 90min with the foregoing description 1-4 can kill the bacillus subtilis black variety gemma that dyes on stainless steel carrier fully, reaches the sterilization standard-required; With its diluent effect 5min of 1: 10 to staphylococcus aureus, colibacillaryly kill the bacterium logarithm value all＞5, reach the disinfection standard requirement.
Preparation method: with ethanol and/or isopropyl alcohol dissolving benzoic acid, add a small amount of pure water after, mixing adds corrosion inhibiter and hydrogen peroxide again, fully use phosphoric acid regulating ph value 1-5 behind the mixing, adds extremely required total amount of pure water again.
Claims (5)
1. disinfection sanitizer, it is characterized in that described disinfectant comprises following component and mass concentration: hydrogen peroxide 4.64-4.80g/100mL, aromatic carboxylic acid 0.05-15g/100mL, mineral acid material 0.05-15g/100mL, the organic inhibitor 0-8g/100mL compatible with hydrogen peroxide, hydrophilic solvent 1-70g/100mL, surplus is a water.
2. a kind of disinfection sanitizer as claimed in claim 1 is characterized in that described disinfectant pH value is 1-5.
3. a kind of disinfection sanitizer as claimed in claim 1 is characterized in that aromatic carboxylic acid is a benzoic acid in the described disinfectant, and its mass concentration is 0.1-8g/100mL.
4. a kind of disinfection sanitizer as claimed in claim 1 is characterized in that the mineral acid material is an inorganic phosphate in the described disinfectant, regulates disinfectant pH value 1-5 with it, and its mass concentration is 0.1-8g/100mL.
5. a kind of disinfection sanitizer as claimed in claim 1 is characterized in that hydrophilic solvent in the described disinfectant is a kind of or its two kinds the mixture in ethanol and the isopropyl alcohol, and its mass concentration is 2-60g/100mL.
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| CNB2006100056125A CN100441229C (en) | 2005-02-18 | 2006-01-02 | Sterilizing and disinfection agent |
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| CN103300060B (en) * | 2013-05-31 | 2016-01-20 | 天津南药科技有限公司 | A kind of hydrogenperoxide steam generator and preparation method thereof |
| CN108209070A (en) * | 2017-12-05 | 2018-06-29 | 钟茜茜 | The production method of deciduous teeth jewelry |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1379983A (en) * | 2001-04-13 | 2002-11-20 | 郭颖 | Compound disinfectant liquor and its preparation method |
| CN1466876A (en) * | 2002-06-21 | 2004-01-14 | �ձ�������ʽ���� | Sterilizing composition and method for sterilizing using the same |
| US20050019421A1 (en) * | 2003-07-23 | 2005-01-27 | 3M Innovative Properties Company | Disinfecting compositions and methods of making and using same |
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2006
- 2006-01-02 CN CNB2006100056125A patent/CN100441229C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1379983A (en) * | 2001-04-13 | 2002-11-20 | 郭颖 | Compound disinfectant liquor and its preparation method |
| CN1466876A (en) * | 2002-06-21 | 2004-01-14 | �ձ�������ʽ���� | Sterilizing composition and method for sterilizing using the same |
| US20050019421A1 (en) * | 2003-07-23 | 2005-01-27 | 3M Innovative Properties Company | Disinfecting compositions and methods of making and using same |
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| Publication number | Publication date |
|---|---|
| CN1820788A (en) | 2006-08-23 |
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