CN100434119C - Method for producing high immunity yolk antibody liquid - Google Patents
Method for producing high immunity yolk antibody liquid Download PDFInfo
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- CN100434119C CN100434119C CNB2006100414479A CN200610041447A CN100434119C CN 100434119 C CN100434119 C CN 100434119C CN B2006100414479 A CNB2006100414479 A CN B2006100414479A CN 200610041447 A CN200610041447 A CN 200610041447A CN 100434119 C CN100434119 C CN 100434119C
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- immunity
- egg
- antibody liquid
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- distilled water
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- 210000002969 egg yolk Anatomy 0.000 title claims abstract description 24
- 239000007788 liquid Substances 0.000 title claims abstract description 16
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 9
- 230000036039 immunity Effects 0.000 title claims description 21
- 241000287828 Gallus gallus Species 0.000 claims abstract description 15
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 13
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 13
- 235000013601 eggs Nutrition 0.000 claims abstract description 13
- 235000013345 egg yolk Nutrition 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 229920001817 Agar Polymers 0.000 claims description 9
- 239000008272 agar Substances 0.000 claims description 9
- 239000012153 distilled water Substances 0.000 claims description 9
- 229960005486 vaccine Drugs 0.000 claims description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- PZJWYUDBXNNVLZ-UHFFFAOYSA-N 1-cyclopropyl-7-(4-ethylpiperazin-1-yl)-6-fluoro-4-oxoquinoline-3-carboxylic acid;hydrochloride Chemical compound Cl.C1CN(CC)CCN1C(C(=C1)F)=CC2=C1C(=O)C(C(O)=O)=CN2C1CC1 PZJWYUDBXNNVLZ-UHFFFAOYSA-N 0.000 claims description 3
- 125000001246 bromo group Chemical group Br* 0.000 claims description 3
- 239000000645 desinfectant Substances 0.000 claims description 3
- 210000003278 egg shell Anatomy 0.000 claims description 3
- 230000001900 immune effect Effects 0.000 claims description 3
- 210000004681 ovum Anatomy 0.000 claims description 3
- 238000009928 pasteurization Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 2
- 230000000249 desinfective effect Effects 0.000 claims description 2
- 235000013330 chicken meat Nutrition 0.000 abstract description 11
- 239000000243 solution Substances 0.000 abstract description 4
- 241000282887 Suidae Species 0.000 abstract description 2
- 230000008021 deposition Effects 0.000 abstract description 2
- 239000012267 brine Substances 0.000 abstract 2
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 abstract 2
- 230000036512 infertility Effects 0.000 abstract 1
- 230000007774 longterm Effects 0.000 abstract 1
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N sodium azide Substances [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 abstract 1
- 238000010255 intramuscular injection Methods 0.000 description 11
- 239000007927 intramuscular injection Substances 0.000 description 11
- 230000037396 body weight Effects 0.000 description 6
- 208000027312 Bursal disease Diseases 0.000 description 4
- 210000003608 fece Anatomy 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 230000002458 infectious effect Effects 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- SPFYMRJSYKOXGV-UHFFFAOYSA-N Baytril Chemical compound C1CN(CC)CCN1C(C(=C1)F)=CC2=C1C(=O)C(C(O)=O)=CN2C1CC1 SPFYMRJSYKOXGV-UHFFFAOYSA-N 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 208000010359 Newcastle Disease Diseases 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 229960000740 enrofloxacin Drugs 0.000 description 2
- 206010013952 Dysphonia Diseases 0.000 description 1
- 208000005577 Gastroenteritis Diseases 0.000 description 1
- 241000218628 Ginkgo Species 0.000 description 1
- 235000011201 Ginkgo Nutrition 0.000 description 1
- 235000008100 Ginkgo biloba Nutrition 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- 206010018762 Grunting Diseases 0.000 description 1
- 208000010473 Hoarseness Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 241000219780 Pueraria Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 206010064097 avian influenza Diseases 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000001669 bursa of fabricius Anatomy 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000000744 eyelid Anatomy 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 231100000001 growth retardation Toxicity 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 208000009305 pseudorabies Diseases 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 239000009705 sanhuang Substances 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The invention discloses a method for producing high-immunity yolk antibody liquid, which comprises the steps of preparing high-immunity eggs, taking yolk, preparing brine, mixing the yolk with the brine, and adding NaN3Filtering and pasteurizing to obtain the highly immune yolk antibody solution. The egg yolk antibody liquid prepared by the invention has the characteristics of sterility, no deposition and long-term normal-temperature storage, can be stored for more than 2 years at normal temperature, and is particularly suitable for chickens or pigs.
Description
Technical field:
The present invention relates to a kind of production method of antibody liquid.
Background technology:
The preparation method of antagonism body fluid generally adopts prepared in laboratory in the prior art, and the technological requirement height can not be preserved by room temperature, and easily deposition can not effectively be applicable to chicken or pig.
Summary of the invention:
The object of the present invention is to provide the production method of the yelk antibody liquid in high immunity that does not a kind ofly deposit, can long-time room temperature preserves.
Technical solution of the present invention is:
A kind of production method of yelk antibody liquid in high immunity is characterized in that: comprise the following steps:
(1) select healthy bird inlay, use the vaccine reinforced immunological, contain corresponding antibodies in the egg that it is produced, it is standby to obtain high-immunity egg;
(2) high-immunity egg that step (1) is obtained after cleaning, disinfecting, is removed eggshell, Ovum Gallus domesticus album, and it is standby to leave and take egg yolk;
(3) in distilled water, add Sal and agar Icing Sugar, boil, stir, the addition of Sal is 0.7～0.95% of a distilled water weight, the addition of agar is 1～1.3% of a distilled water weight, after treating that agar dissolves fully, cool to 40～50 ℃, and by the ratio adding Enrofloxacin hydrochloride. of 0.3～0.4mg/ml, stirring and dissolving, it is standby to get saline;
(4) 40～50 ℃ saline obtaining of egg yolk that step (2) is obtained and step (3) is by 1: 2～4 mixed, and adds the NaN of final concentration 0.1～0.2%
3, filtration, pasteurization sterilization obtain yelk antibody liquid in high immunity.
The concrete grammar that high-immunity egg is cleaned, disinfected in the step (2) is: high-immunity egg flowing water is cleaned, put into bromo geramine or 84 disinfectant solution and soaked 5～15 minutes.
The yolk antibody liquid that the present invention makes has characteristics aseptic, that do not deposit, can long-time room temperature preserve, can preserve more than 2 years room temperature under, is particularly suitable for the use of chicken or pig.
The concrete example that uses:
Case one, in August, 1996,3000 plumage AA chickens of Semen Ginkgo village, Dong She town, Tongzhou, Jiangsu Province Qin raising, sudden onset during 24 ages in days, the food of mining massively entirely descends, drinking-water rises, draw white dung, feather is contrary upright, large quantities of death, be diagnosed as infectious bursal disease through veterinary station laboratory, Tongzhou, use yolk antibody intramuscular injection 2ml/ plumage of the present invention, be clearly better after 1 day, recover normal after 3 days.
Case two, in April, 2000, the symptom similar to case one takes place in so-and-so 7500 plumage SANHUANG broiler, 30 ages in days of raising of Xin Lian town, Tongzhou, Jiangsu Province utmost point filial piety village Wu, be diagnosed as infectious bursal disease through veterinary station laboratory, Tongzhou, use yolk antibody intramuscular injection 1.5ml/ plumage of the present invention, be clearly better after 1 day, recover normal after 3 days.
Case three, in February, 2002,4000 plumage Luo Man laying hens, the 35 ages in days morbidity of so-and-so raising of Dong Chang village, bank town Pueraria lobota is ridden the Tongzhou in Jiangsu Province, the symptom similar to case one, be diagnosed as infectious bursal disease through veterinary station laboratory, Tongzhou, use yolk antibody intramuscular injection 1ml/ plumage of the present invention, be clearly better after 1 day, recover normal after 3 days.
Case four, in JIUYUE, 2004, the 2800 plumage AA chickens that so-and-so raising of Nan Cun Shen is mended in the town are mended in sky, Haimen City, Jiangsu Province, fall ill during 38 ages in days, draw green dung, slightly breathe the hello sound, the part chicken is turned round nervous symptoms such as neck, paralysed foot, dead constantly increase, be diagnosed as chronic newcastle disease through veterinary station laboratory, Haimen City, use yolk antibody intramuscular injection 2.5ml/ plumage of the present invention, injected once in second day again, chicken group mental status was clearly better in the 3rd day, feed intake rises significantly, death no longer increases, and death in the 5th day begins to descend, and stops death on the 8th day substantially.
Xin Lian town, Tongzhou, Jiangsu Province take so-and-so clinic from used in 1994 chicken of the present invention with the multivalence high immunity yolk antibody since, use every year about 500,000 ml approximately, about treatment chicken group 250,000 plumages, 10 years about 5,000,000 ml, 2,500,000 plumage chickens, rough estimates are 100% to the effective percentage of infectious bursal disease, cure rate about about 95%, effective percentage to newcastle disease is 90%, and cure rate is about about 70%, and effect is satisfactory.
Case five, in November, 2004, one circle of so-and-so raising of Luan Dian village, town, dan port, Tongzhou, the Jiangsu Province Yin Dynasty is from numerous piglet, 7 ages in days begin to draw yellow dung, acute death, and it is little to use multiple antibiotics effect, be diagnosed as yellow scour of piglet through veterinary station laboratory, Tongzhou, use pig of the present invention with multivalence high immunity yolk antibody intramuscular injection 0.5ml/ kg body weight, injected once again, and cooperated the Enrofloxacin Base intramuscular injection simultaneously in the 2nd day, stop to have loose bowels after 2 days, stop death after 3 days.This disease does not take place in 1 age in days and twice per kilogram of body weight intramuscular injection of 10 ages in days high immunity yolk antibody 0.5ml again after every batch of piglet is born later on.
Case six, in December, 2004, a circle of dan port, Tongzhou, Jiangsu Province town Dong Shengqiao village Zhang raising is from numerous piglet, and 30 ages in days begin to draw white dung, use multiple antibiotics and do not take effect, delay and to take a turn for the better in more than 10 day, become thin, growth retardation, fragmentary dead, be diagnosed as piglet pujos blancos through veterinary station laboratory, Tongzhou, use pig of the present invention and use multivalence high immunity yolk antibody intramuscular injection 0.5ml/ kg body weight, injection was once again in the day, cooperate the Enrofloxacin Base intramuscular injection simultaneously, take a turn for the better after 2 days, cure after 5 days.This disease does not take place in 1 age in days and twice per kilogram of body weight intramuscular injection of 10 ages in days high immunity yolk antibody 0.5ml again after every batch of piglet is born later on.
Case seven, in October, 2005, so-and-so two circles of raising of Dong Chang village, bank town king are ridden the Tongzhou from numerous piglet by Jiangsu Province, sudden onset during a circle 60 ages in days wherein, and large quantities of batches of death of non-evident sympton, the minority dead sick grunting of hogs hoarseness that is not able to do in time is mute, eyelid swelled.Be diagnosed as baby pig edema through veterinary station laboratory, Tongzhou, adopt aggregate measures such as anti-inflammation, diuresis, toxin expelling, death toll head again, the state of an illness was controlled in the 5th day.Another encloses also sudden onset after one week, and symptom is identical, so the high immunity yolk antibody 0.5ml/ kg body weight of annotating on the basis of above-mentioned aggregate measures was injected once on the 2nd day again, just cures only dead 1 on the 3rd day.
This disease does not take place in 1 age in days and twice per kilogram of body weight intramuscular injection of 10 ages in days high immunity yolk antibody 0.5ml again after every batch of piglet is born later on.
The invention will be further described below in conjunction with embodiment.
The specific embodiment:
A kind of production method of yelk antibody liquid in high immunity comprises the following steps:
(1) select healthy bird inlay, adopt simultaneously or in batches reinforced immunological repeatedly of conventional method with vaccine, contain corresponding antibodies in the egg that it is produced, it is standby to obtain high-immunity egg.When wherein using yelk antibody liquid in high immunity for the preparation chicken, the vaccine immunity (vaccine adopts commercially available prod or conventional method self-control product) that can select for use related antigens such as newcastle, fabricius bursa, biography are propped up, bird flu, Mycoplasma, escherichia coli to make; When using yelk antibody liquid in high immunity for the preparation pig, the vaccine immunity (vaccine adopts commercially available prod or conventional method self-control product) that can select for use related antigens such as escherichia coli, transmissible gastroenteritis, epidemic diarrhea, reproductive and respiratory syndrome, pseudorabies to make.
(2) high-immunity egg that step (1) is obtained, flowing water is cleaned, and puts into bromo geramine or 84 disinfectant solution and soaks 5～15 minutes (example 5 minutes, 10 minutes, 15 minutes), removes eggshell, Ovum Gallus domesticus album again, and it is standby to leave and take egg yolk;
(3) in distilled water, add Sal and agar Icing Sugar, boil, stir, the addition of Sal is 0.7～0.95% (example 0.7%, 0.85%, 0.95%) of distilled water weight, the addition of agar is 1～1.3% (example 1%, 1.12%, 1.3%) of distilled water weight, after treating that agar dissolves fully, cool to 40～50 ℃, and by the ratio adding Enrofloxacin hydrochloride. of 0.3mg/ml (or 0.35mg/ml, 0.4mg/ml), stirring and dissolving, it is standby to get saline;
(4) 40～50 ℃ saline obtaining of egg yolk that step (2) is obtained and step (3) is by the mixed of 1: 2～4 (examples 1: 2,1: 3,1: 4), obtains mixed liquor and adds the NaN of final concentration 0.1～0.2% (example 0.1%, 0.15%, 0.2%)
3(be the NaN in the final mixed liquor
3Concentration is 0.1～0.2%), filtration, pasteurization sterilization obtain yelk antibody liquid in high immunity.
Claims (2)
1, a kind of production method of yelk antibody liquid in high immunity is characterized in that: comprise the following steps:
(1) it is standby to take the high-immunity egg that healthy bird inlay produced, that contain corresponding antibodies that the vaccine reinforced immunological crosses;
(2) with the high-immunity egg of step (1), after cleaning, disinfecting, remove eggshell, Ovum Gallus domesticus album, it is standby to leave and take egg yolk;
(3) in distilled water, add Sal and agar Icing Sugar, boil, stir, the addition of Sal is 0.7～0.95% of a distilled water weight, the addition of agar is 1～1.3% of a distilled water weight, after treating that agar dissolves fully, cool to 40～50 ℃, and by the ratio adding Enrofloxacin hydrochloride. of 0.3～0.4mg/ml, stirring and dissolving, it is standby to get saline;
(4) 40～50 ℃ saline obtaining of egg yolk that step (2) is obtained and step (3) is by 1: 2～4 mixed, and adds the NaN of final concentration 0.1～0.2%
3, filtration, pasteurization sterilization obtain yelk antibody liquid in high immunity.
2, the production method of yelk antibody liquid in high immunity according to claim 1 is characterized in that: the concrete grammar that high-immunity egg is cleaned, disinfected in the step (2) is: high-immunity egg flowing water is cleaned, put into bromo geramine or 84 disinfectant solution and soaked 5～15 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100414479A CN100434119C (en) | 2006-09-04 | 2006-09-04 | Method for producing high immunity yolk antibody liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100414479A CN100434119C (en) | 2006-09-04 | 2006-09-04 | Method for producing high immunity yolk antibody liquid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1915426A CN1915426A (en) | 2007-02-21 |
| CN100434119C true CN100434119C (en) | 2008-11-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100414479A Expired - Fee Related CN100434119C (en) | 2006-09-04 | 2006-09-04 | Method for producing high immunity yolk antibody liquid |
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| Country | Link |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1439650A (en) * | 2002-04-08 | 2003-09-03 | 邱义能 | Preparation of multi-antigen from waterfowl |
| CN1463985A (en) * | 2002-06-12 | 2003-12-31 | 顾德生物科技股份有限公司 | Process for selectively separating IgY antibody from anseriforme birds eggs and IgY antibody produced therefrom |
-
2006
- 2006-09-04 CN CNB2006100414479A patent/CN100434119C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1439650A (en) * | 2002-04-08 | 2003-09-03 | 邱义能 | Preparation of multi-antigen from waterfowl |
| CN1463985A (en) * | 2002-06-12 | 2003-12-31 | 顾德生物科技股份有限公司 | Process for selectively separating IgY antibody from anseriforme birds eggs and IgY antibody produced therefrom |
Non-Patent Citations (1)
| Title |
|---|
| 高免鸡蛋卵黄囊抗体的提取与纯化. 吴周良等.河北畜牧兽医，第21卷第7期. 2005 * |
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| CN1915426A (en) | 2007-02-21 |
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