CN100383183C - Algae ferment hydrolysate and its producing method - Google Patents
Algae ferment hydrolysate and its producing method Download PDFInfo
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- CN100383183C CN100383183C CNB2004101006139A CN200410100613A CN100383183C CN 100383183 C CN100383183 C CN 100383183C CN B2004101006139 A CNB2004101006139 A CN B2004101006139A CN 200410100613 A CN200410100613 A CN 200410100613A CN 100383183 C CN100383183 C CN 100383183C
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Abstract
The present invention relates to an algae ferment hydrolysate and a producing method thereof. The algae ferment hydrolysagte is an undissolved precipitate which is obtained by making dewatering algae processed by condensing, and accordingly, the algae ferment hydrolysate which can dissolve and has biotic activity is obtained by making use of the specific condition processing of ferment reaction. Since the algae ferment hydrolysate of the present invention at least comprises a mixture of polysaccharide and polypeptide, the present invention can not only effectively promote the growth of skin cells of extrasomatic cultivation, but also can be added into a formula of constituents of cosmetics, food products, or external pharmaceuticals of skin.
Description
Technical field
The present invention relates to a kind of algae hydrolyzate and manufacture method thereof, particularly relate to algae protease hydrolysate and the manufacture method thereof handled through enzyme.
Background technology
The algae of protobiont is the basic unit of fresh water and marine ecosystem food web, can be hydrocoles energy and nutrition are provided.Relatively large algae can be for human edible, and more small-sized algae, Spirullina (Spirulina spp.) of the Chlorella of green alga (Chlorella spp.), blue-green algae etc. for example, because rich in proteins, the A wide selection of colours and designs and the equilibrium of human indispensable amino acid, VITAMIN, polybasic unsaturated fatty acid and micronutrient levels are all very abundant, so become heath food via artificial a large amount of the cultivation.
Secondly, many results of study are pointed out lipopolysaccharide class (Lipopolysaccharide) contained in the spirulina and anthocyanidin (Phycocyanin) but have to strengthen marrow regeneration, thymus gland become serum protein and enhancing immunity system with spleen growth intercrescence function.Moreover the spirulina in water hot extraction's thing of spirulina (Spirulina platensis) contains calcareous algae polysaccharide (Calcium Spirulan; Ca-SP) have more inhibition hsv (Herpes SimplexVirus; HSV) with HIV (human immunodeficiency virus) (Human Immunodeficiency Virus; HIV) antiviral efficacy (No. the 5th, 585,365, United States Patent (USP) notification number).In addition, also there are many results of study to point out that composition by the algae extraction more has the diabetes of alleviating and hypertension, reducing cholesterol, effect such as anticancer.
In addition, frond itself, frond extract or its hydrolyzate (Hydrolysate) more can be added in the makeup, to promote various beauty functions, for example the Taiwan patent announcement is number No. 520286, No. the 2003/0091560th, Application No., No. the 2002/0160064th, Application No., No. the 2002/0120242nd, Application No., United States Patent (USP) notification number the 6th, 190, No. 664, the 5th, 508, No. 033, European patent notification number the 1st, 239, No. 813, Russian Federation's patent announcement number the 2nd, 114, No. 632, Chinese patent notification number the 1st, 206, No. 587, French Patent notification number the 2nd, 609, No. 246, French Patent notification number the 2nd, 555, No. 444 and Japan's special permission notification number the 52nd, 021, No. 336 etc., more than all classify reference of the present invention as.Above-mentioned patent is to disclose the various makeup that contain the spirulina extract to form, the active isoflavones (Isoflavone Aglycone) of at least a desaccharification body of for example cryodesiccated spirulina powder, chlorella and spirulina extract, spirulina extraction, via freeze-thaw circulation and sodium chloride solution extraction and the spirulina extract, via the water hot extraction the spirulina extract and through the spirulina hydrolyzate of organic solvent ethanol/acetone and ethanol/water extraction etc.
From the above, the existing technology of using algae do not take off in direct application frond, via freeze-thaw circulation and sodium chloride solution extraction and the algae extract, via organic solvent extraction the algae hydrolyzate or via the water hot extraction the algae extract.Yet frond itself is difficult for being absorbed by human body skin, and via in freeze-thaw circulation, organic solvent extraction or the water hot extraction's process, destroys the contained biologically active substance of algae easily.
Summary of the invention
One of purpose of the present invention is exactly to disclose a kind of algae protease hydrolysate, this algae protease hydrolysate comprises the mixture of polysaccharide (Polysaccharide) and polypeptide (Polypeptide) at least, can be added in the prescription of makeup, food or external preparation for skin pharmaceuticals constituent.
Another object of the present invention is to disclose a kind of manufacture method of algae protease hydrolysate, this algae protease hydrolysate be with the dehydration algae through rehydration (Rehydration) handle the back and the throw out (Pellet) that do not dissolve utilize specific enzyme reaction condition processing, obtain the algae protease hydrolysate of solubilized and tool biological activity (Bioactive) whereby, wherein aforementioned algae protease hydrolysate comprises the mixture of polysaccharide and polypeptide at least.
Purpose of the present invention can be achieved through the following technical solutions:
A kind of algae protease hydrolysate (Enzymatic Hydrolysate), it is characterized in that, this algae protease hydrolysate be with dehydration (Dehydrated) algae through rehydration (Rehydration) handle the back and must the throw out that do not dissolve utilize endopeptidase (Endopeptidase) in room temperature treatment 1.5 hours to 16 hours, obtain the algae protease hydrolysate of solubilized and tool biological activity (Bioactive) whereby, the algae that wherein dewaters is selected from the group that is made up of Chlorella (Chlorella spp.) and Spirullina (Spirulina spp.), and the algae protease hydrolysate comprises polysaccharide (Polysaccharide) and molecular weight between 1000 dalton (Dalton; Da) to the mixture of the polypeptide (Polypeptide) of 17000 Da.
Described endopeptidase is to be selected from by stomach en-(Pepsin), trypsin Trypsin), the group that forms of papain (Papain) and other proteolytic ferments.
The described throw out that do not dissolve is to utilize stomach en-to handle between 1 to 5 in (about 25 ℃) and pH-value (pH) under the room temperature.
The described throw out that do not dissolve is to utilize this trypsinase to handle between 5 to 9 in (about 25 ℃) and pH-value under the room temperature.
The described throw out that do not dissolve is to utilize papain to handle between 5 to 10 between (about 25 ℃) and pH-value under the room temperature in temperature.
The effective constituent or the food additives of the additive that described algae protease hydrolysate is a cosmetic composition, external preparation for skin pharmaceuticals constituent.
Described additive is to make an addition in the matrix with weight percent weight percent between 0.1 weight percent to 50, and this matrix is to be selected from a group that is made up of breast frost (Cream), emulsion (Lotion), essence (Essence) and a gel (Gel).
A kind of manufacture method of algae protease hydrolysate is characterized in that, comprises at least:
The dehydration algae is carried out rehydration handle, obtaining supernatant liquor and not dissolve throw out, wherein said dehydration algae is to be selected from the group that is made up of Chlorella and Spirullina; Remove this supernatant liquor; And, this is not dissolved throw out utilizes endopeptidase in room temperature treatment 1.5 hours to 16 hours, obtain the bioactive algae protease hydrolysate of solubilized and tool whereby, wherein said algae protease hydrolysate comprises polysaccharide and the molecular weight mixture between the polypeptide of 1000 Da to 17000 Da.
Described endopeptidase is selected from the group that is made up of stomach en-, trypsinase, papain and other proteolytic ferments.
The described throw out that do not dissolve is to utilize stomach en-to handle between 1 to 5 in (about 25 ℃) and pH-value under the room temperature.
The described throw out that do not dissolve is to utilize trypsinase to handle between 5 to 9 in (about 25 ℃) and pH-value under the room temperature.
The described throw out that do not dissolve is to utilize papain to handle between 5 to 10 in (about 25 ℃) and pH-value under the room temperature.
The above-mentioned purpose according to the present invention, a kind of algae protease hydrolysate is proposed, this algae protease hydrolysate is with the dehydration algae, the algae powder of Chlorella or Spirullina for example, through rehydration handle the back and must the throw out that do not dissolve utilized specific enzyme reaction condition and room temperature treatment 1.5 hours to 16 hours, obtain the bioactive algae protease hydrolysate of solubilized and tool whereby, wherein aforementioned algae protease hydrolysate comprises polysaccharide and molecular weight between 1000 dalton (Dalton; Da) to the mixture of the polypeptide of 17000 Da.
According to preferred embodiment of the present invention, employed enzyme is endopeptidase (Endopeptidase) in the above-mentioned specific enzyme reaction condition.
According to preferred embodiment of the present invention, above-mentioned algae protease hydrolysate can be added in the prescription of makeup, food or external preparation for skin pharmaceuticals constituent.
In addition, according to the present invention on also another purpose, a kind of manufacture method of algae protease hydrolysate is proposed.At first, with the dehydration algae for example the algae powder of Chlorella or Spirullina carry out can obtaining solubilized supernatant liquor (Supernatant) and not dissolving throw out after rehydration handles.After removing supernatant liquor, utilize specific enzyme reaction condition in room temperature treatment 1.5 hours to 16 hours the aforementioned throw out that do not dissolve, obtain the bioactive algae protease hydrolysate of solubilized and tool whereby, wherein aforementioned algae protease hydrolysate comprises polysaccharide and the molecular weight mixture between the polypeptide of 1000 Da to 17000 Da.
Use the algae protease hydrolysate of above-mentioned manufacture method gained, owing to being handles the throw out that do not dissolve that the back gets with the dehydration algae through rehydration to utilize specific enzyme reaction condition processing again, obtain the bioactive algae protease hydrolysate of solubilized and tool whereby, wherein above-mentioned algae protease hydrolysate comprises the mixture of polysaccharide and polypeptide at least, can be added in the prescription of makeup, food or external preparation for skin pharmaceuticals constituent.
Description of drawings
Fig. 1 is the skin cells allometry situation of the interpolation algae protease hydrolysate of several preferred embodiments according to the present invention.
Embodiment
Algae protease hydrolysate of the present invention and manufacture method thereof, be with the dehydration algae through rehydration handle the back and the throw out that do not dissolve utilize specific enzyme reaction condition processing again, obtain the bioactive algae protease hydrolysate of solubilized and tool whereby.At first, with the dehydration algae for example the algae powder of Chlorella or Spirullina under room temperature, carry out rehydration and handle, being about to aforementioned algae powder is suspended in distilled water, deionized water, other purified water or the buffered soln, to obtain algae suspension, wherein the concentration of algae suspension used in the present invention is not limit, and looks closely demand and decides.In an example, the concentration of employed algae suspension for example can include but not limited to wherein to comprise the solubilized supernatant liquor and not dissolve throw out between 20 weight percent to 40 weight percents.After utilizing centrifugal or mode such as filtration remove supernatant liquor, utilize specific enzyme reaction condition in room temperature treatment 1.5 hours to 16 hours the aforementioned throw out that do not dissolve, obtain the bioactive algae protease hydrolysate of solubilized and tool whereby.
According to a preferred embodiment of the present invention, employed enzyme is an endopeptidase in the above-mentioned specific enzyme reaction condition, so with stomach en-(Pepsin), trypsin Trypsin), papain (Papain) or other proteolytic ferments be preferable.In an example, when not dissolving throw out and utilizing pepsin, preferable enzyme reaction condition is to handle between 1 to 5 between (about 25 ℃) and pH-value (pH) under the room temperature in temperature with the stomach en-of 0.15 weight percent to 10 weight percent when above-mentioned.In another example, when the above-mentioned throw out that do not dissolve utilizes trypsinase, during processing, preferable enzyme reaction condition is to handle between 5 to 9 between (about 25 ℃) and pH-value under the room temperature in temperature with the trypsinase of 0.15 weight percent to 10 weight percent.In another example, when not dissolving throw out and utilizing papain to handle, preferable enzyme reaction condition is to handle between 5 to 10 between (about 25 ℃) and pH-value under the room temperature in temperature with the papain of 0.15 weight percent to 10 weight percent when above-mentioned.
After handling through above-mentioned endopeptidase, algae protease hydrolysate one polysaccharide of gained and molecular weight are between the mixture of the polypeptide of 1000 Da to 17000 Da, not only effectively promote the growth of the skin cells of vitro culture (In Vitro), more can be added in the prescription of makeup, food or external preparation for skin pharmaceuticals constituent.
Below enumerate several preferred embodiments with the more application of elaboration algae protease hydrolysate of the present invention and manufacture method thereof, so it is not in order to qualification the present invention, so protection scope of the present invention is as the criterion when looking the accompanying Claim person of defining.
Embodiment 1
At first, the dehydration spirulina powder is carried out can obtaining supernatant liquor and not dissolving throw out after rehydration is processed into the spirulina solution of 28 weight percents for example.After removing supernatant liquor, do not dissolve in the buffered soln that throw out is dissolved in pH-value about 2.5 again aforementioned, the stomach en-that utilizes 0.57 weight percent for example is under room temperature (about 25 ℃), after handling 0.5 hour, 1.0 hours, 1.5 hours, 2.0 hours and 16.0 hours respectively, end pepsic reaction immediately, obtain the algae protease hydrolysate whereby.
Embodiment 2
At first, the dehydration spirulina powder is carried out can obtaining supernatant liquor and not dissolving throw out after rehydration is processed into the spirulina solution of 28 weight percents for example.After removing supernatant liquor, do not dissolve in the buffered soln that throw out is dissolved in pH-value about 3.5 again aforementioned, the stomach en-that utilizes 0.57 weight percent for example is under room temperature (about 25 ℃), after handling 0.5 hour, 1.0 hours, 1.5 hours, 2.0 hours and 16.0 hours respectively, end pepsic reaction immediately, obtain the algae protease hydrolysate whereby.
Embodiment 3
At first, the dehydration spirulina powder is carried out can obtaining supernatant liquor and not dissolving throw out after rehydration is processed into the spirulina solution of 30 weight percents for example.After removing supernatant liquor, the aforementioned throw out that do not dissolve is dissolved in about 6.5 to 7.5 the buffered soln of pH-value again, the papain that utilizes 0.57 weight percent for example is under room temperature (about 25 ℃), after handling 0.5 hour, 1.0 hours, 1.5 hours, 2.0 hours and 16.0 hours respectively, end the reaction of papain immediately, obtain the algae protease hydrolysate whereby.
Embodiment 4
At first, the dehydration spirulina powder is carried out can obtaining supernatant liquor and not dissolving throw out after rehydration is processed into the spirulina solution of 35 weight percents for example.After removing supernatant liquor, the aforementioned throw out that do not dissolve is dissolved in about 6.5 to 7.5 the buffered soln of pH-value again, the papain that utilizes 1 weight percent for example is under about 50 ℃, after handling 0.5 hour, 1.0 hours, 1.5 hours, 2.0 hours and 16.0 hours respectively, end the reaction of papain immediately, obtain the algae protease hydrolysate whereby.
Embodiment 5
At first, the dehydration spirulina powder is carried out can obtaining supernatant liquor and not dissolving throw out after rehydration is processed into the spirulina solution of 25 weight percents for example.After removing supernatant liquor, the aforementioned throw out that do not dissolve is dissolved in about 6.5 to 7.5 the buffered soln of pH-value again, the trypsinase that utilizes 0.57 weight percent for example is under room temperature (about 25 ℃), after handling 0.5 hour, 1.0 hours, 1.5 hours, 2.0 hours and 16.0 hours respectively, end tryptic reaction immediately, obtain the algae protease hydrolysate whereby.
Embodiment 6
At first, the dehydration spirulina powder is carried out can obtaining supernatant liquor and not dissolving throw out after rehydration is processed into the spirulina solution of 20 weight percents for example.After removing supernatant liquor, do not dissolve in the buffered soln that throw out is dissolved in pH-value about 9.0 again aforementioned, the trypsinase that utilizes 0.25 weight percent for example is under room temperature (about 25 ℃), after handling 0.5 hour, 1.0 hours, 1.5 hours, 2.0 hours and 16.0 hours respectively, end tryptic reaction immediately, obtain the algae protease hydrolysate whereby.
Embodiment 7
The various algae protease hydrolysates of embodiment 1 to embodiment 6 gained are through the protein electrophorese gel analysis, and the molecular weight of the polypeptide of gained is between for example (figure does not illustrate) between 1000 Da to 17000 Da.Then, with the various algae protease hydrolysates of embodiment one to embodiment six gained, carry out the growth test of vitro culture skin cells.Generally speaking, the skin cells of test usefulness can adopt the mammals fibroblast, is example with embodiment seven, is that the strain of rodents 3T3 fibroblast is incubated in 96 porose discs, with the about for example cell density of 1 * 105 cell count in every hole, carry out the growth test of vitro culture skin cells.Algae protease hydrolysate with embodiment one to embodiment six gained, make an addition in the cell culture fluid respectively with the concentration between 1 volume percent to 10 volume percent, under about 37 ℃, the environment of 5% gas concentration lwevel, after cultivating 48 hours, utilize MTT test (3-[4,5-Dimethylthylthiazol-2-yl]-2,5-DiphenyltetrazoliumBromide Assay; MTT Assay, 3 (4,5-dimethylthiazole-2)-2,5-phenylbenzene tetrazole bromine salt test) come the situation of quantitative analysis cell growth.The cell culture fluid of control group does not then add the algae protease hydrolysate.See also table 1, be the survivaling cell per-cent that shows the interpolation algae protease hydrolysate of several preferred embodiments according to the present invention, wherein the survivaling cell per-cent of following each embodiment is that the survivaling cell number with control group is 100% to get after stdn (Normalization).
Table 1
The result of above-mentioned table 1 further represents with Fig. 1.See also Fig. 1, it is the skin cells allometry situation of the interpolation algae protease hydrolysate of several preferred embodiments according to the present invention, wherein the longitudinal axis is represented the survivaling cell per-cent (%) with respect to control group, and transverse axis is represented the algae protease hydrolysate of embodiment 1 to embodiment 6 gained of handling through different time from left to right in regular turn.Rectangular 101, rectangular 103, rectangular 105, rectangular 107 and rectangular 109 in regular turn respectively expression add survivaling cell per-cent behind the algae protease hydrolysate of embodiment 1 treated 0.5 hour, 1.0 hours, 1.5 hours, 2.0 hours and 16.0 hours.Rectangular 111, rectangular 113, rectangular 115, rectangular 117 and rectangular 119 in regular turn respectively expression add survivaling cell per-cent behind the algae protease hydrolysate of embodiment 2 treated 0.5 hour, 1.0 hours, 1.5 hours, 2.0 hours and 16.0 hours.Rectangular 121, rectangular 123, rectangular 125, rectangular 127 and rectangular 129 in regular turn respectively expression add survivaling cell per-cent behind the algae protease hydrolysate of embodiment 3 treated 0.5 hour, 1.0 hours, 1.5 hours, 2.0 hours and 16.0 hours.Rectangular 131, rectangular 133, rectangular 135, rectangular 137 and rectangular 139 in regular turn respectively expression add survivaling cell per-cent behind the algae protease hydrolysate of embodiment 4 treated 0.5 hour, 1.0 hours, 1.5 hours, 2.0 hours and 16.0 hours.Rectangular 141, rectangular 143, rectangular 145, rectangular 147 and rectangular 149 in regular turn respectively expression add survivaling cell per-cent behind the algae protease hydrolysate of embodiment 5 treated 0.5 hour, 1.0 hours, 1.5 hours, 2.0 hours and 16.0 hours.Rectangular 151, rectangular 153, rectangular 155, rectangular 157 and rectangular 159 in regular turn respectively expression add survivaling cell per-cent behind the algae protease hydrolysate of embodiment 6 treated 0.5 hour, 1.0 hours, 1.5 hours, 2.0 hours and 16.0 hours.
As table 1 and shown in Figure 1, by and large, at the algae protease hydrolysate that adds after stomach en-, trypsinase or papain are handled at least 1.5 hours, can effectively promote the growth of the 3T3 fibroblast of vitro culture, its survivaling cell per-cent has more about 30% than control group and does not wait to about 60%, wherein the effect of embodiment five and the embodiment 6 3T3 fibroblast growth that utilizes trypsin treatment promptly to have in 1.0 hours to promote vitro culture.
In brief, the present invention be with the dehydration algae through rehydration handle the back and the throw out that do not dissolve utilize specific enzyme reaction condition processing again, obtain the bioactive algae protease hydrolysate of solubilized and tool whereby.What deserves to be mentioned is that the algae protease hydrolysate that the present invention makes not only effectively promotes the growth of the skin cells of vitro culture, more can be added in the prescription of makeup, food or external preparation for skin pharmaceuticals constituent.With the cosmetic composition is example, the algae protease hydrolysate that the present invention makes can make an addition in the matrix between 0.1 weight percent to 50 weight percent by content, and matrix can be for example breast frost (Cream), emulsion (Lotion), essence (Essence) or gel (Gel) etc.
By the invention described above preferred embodiment as can be known, use algae protease hydrolysate of the present invention, its advantage is that this algae protease hydrolysate comprises the mixture of polysaccharide and polypeptide at least, not only effectively promote the growth of the skin cells of vitro culture, more can be applied to the effective constituent or the food additives of makeup and external preparation for skin pharmaceuticals constituent.
By the invention described above preferred embodiment as can be known, use the manufacture method of algae protease hydrolysate of the present invention, its advantage be this algae protease hydrolysate be with the dehydration algae through rehydration handle the back and the throw out that do not dissolve utilize specific enzyme reaction condition processing, obtain the bioactive algae protease hydrolysate of solubilized and tool whereby.Because above-mentioned algae protease hydrolysate comprises the mixture of polysaccharide and polypeptide at least, can effectively promote the growth of the skin cells of vitro culture.
Though the present invention discloses as above with several preferred embodiments; right its is not in order to limit the present invention; anyly have the knack of this skill person; without departing from the spirit and scope of the present invention; when can being used for a variety of modifications and variations, so protection scope of the present invention is when looking being as the criterion that accompanying Claim defines.
Claims (11)
1. algae protease hydrolysate, it is characterized in that, this algae protease hydrolysate be with the dehydration algae through rehydration handle the back and must the throw out that do not dissolve utilize endopeptidase in room temperature treatment 1.5 hours to 16 hours, obtain the bioactive algae protease hydrolysate of solubilized and tool whereby, the algae that wherein dewaters is selected from the group that is made up of Chlorella and Spirullina, and the algae protease hydrolysate comprises polysaccharide and molecular weight between the mixture of 1000 dalton to the polypeptide of 17000Da.
2. algae protease hydrolysate as claimed in claim 1 is characterized in that, described endopeptidase is to be selected from the group that is made up of stomach en-, trypsinase, papain and other proteolytic ferments.
3. algae protease hydrolysate as claimed in claim 2 is characterized in that, the described throw out that do not dissolve is to utilize stomach en-to handle between 1 to 5 with pH-value.
4. algae protease hydrolysate as claimed in claim 2 is characterized in that, the described throw out that do not dissolve is to utilize this trypsinase to handle between 5 to 9 with pH-value.
5. algae protease hydrolysate as claimed in claim 2 is characterized in that, the described throw out that do not dissolve is to utilize papain in handling between 5 to 10 with pH-value.
6. algae protease hydrolysate as claimed in claim 1 is characterized in that, described algae ferment hydrolysate is the additive of cosmetic composition, the effective constituent or the food additives of external preparation for skin pharmaceuticals constituent;
Described additive is to make an addition in the matrix with weight percent weight percent between 0.1 weight percent to 50, and this matrix is to be selected from the group that is made up of breast frost, emulsion, essence and gel.
7. the manufacture method of an algae protease hydrolysate is characterized in that, comprises at least:
The dehydration algae is carried out rehydration handle, obtaining supernatant liquor and not dissolve throw out, wherein said dehydration algae is to be selected from the group that is made up of Chlorella and Spirullina; Remove this supernatant liquor; And, this is not dissolved throw out utilizes endopeptidase in room temperature treatment 1.5 hours to 16 hours, obtain the bioactive algae protease hydrolysate of solubilized and tool whereby, wherein said algae protease hydrolysate comprises polysaccharide and the molecular weight mixture between the polypeptide of 1000Da to 17000Da.
8. the manufacture method of algae protease hydrolysate as claimed in claim 7 is characterized in that, described endopeptidase is selected from the group that is made up of stomach en-, trypsinase, papain and other proteolytic ferments.
9. the manufacture method of algae protease hydrolysate as claimed in claim 8 is characterized in that, the described throw out that do not dissolve is to utilize stomach en-to handle between 1 to 5 with pH-value.
10. the manufacture method of algae protease hydrolysate as claimed in claim 8 is characterized in that, the described throw out that do not dissolve is to utilize trypsinase to handle between 5 to 9 with pH-value.
11. the manufacture method of algae protease hydrolysate as claimed in claim 8 is characterized in that, the described throw out that do not dissolve is to utilize papain to handle between 5 to 10 with pH-value.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1218636A (en) * | 1997-11-27 | 1999-06-09 | 中国科学院水生生物研究所 | Extracting method for nutritive component of spirulina |
| CN1367257A (en) * | 2002-02-08 | 2002-09-04 | 江苏省农业科学院土壤肥料研究所 | Extraction method of chlorella growth factor (chlorella extract and CGF) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1218636A (en) * | 1997-11-27 | 1999-06-09 | 中国科学院水生生物研究所 | Extracting method for nutritive component of spirulina |
| CN1367257A (en) * | 2002-02-08 | 2002-09-04 | 江苏省农业科学院土壤肥料研究所 | Extraction method of chlorella growth factor (chlorella extract and CGF) |
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