CN1218636A - Extracting method for nutritive component of spirulina - Google Patents
Extracting method for nutritive component of spirulina Download PDFInfo
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- CN1218636A CN1218636A CN97109320A CN97109320A CN1218636A CN 1218636 A CN1218636 A CN 1218636A CN 97109320 A CN97109320 A CN 97109320A CN 97109320 A CN97109320 A CN 97109320A CN 1218636 A CN1218636 A CN 1218636A
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- spirulina
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Abstract
The stepped extracting technology of spirulina nutrients includes freeze thawing, combined enzymolysis, hot water extraction and other processes. The technology can extract fully and the obtained nutrient liquid can be used separately or in coordination for producing various beverage, oral liquid and additive for cosmetics. The extraction process uses no acid, alkali or organic solvent, produces no environmental pollution, and is easy to operate and to be used in industrial production.
Description
The present invention relates to the extraction of algal nutrient matter, particularly the extractive technique of nutritive component of spirulina.
Nutritive component of spirulina is comprehensive, has the good health care effect, is confirmed by domestic and international researcher.Spirulina is acknowledged as " super nutritive food " in the United Nations's World Food Summit in 1974, confirmed as " optimum protein matter source " by FDA (FDA) in 1981, and be described as by FAO (Food and Agriculture Organization of the United Nation) (FAO) " optimal food tomorrow ".At present both at home and abroad existing about various schools of thinkers spirulina manufacturer, its major product is a spirulina powder.
Chinese patent literature: 1, nutritious algae liquid and making thereof and purposes, classification number: A61K7/48, the applying date: 94.07.28, the day for announcing: 96.01.31, application number: 94107892, applicant: Shipurui Co. Ltd., Yunnan Prov..The emphasis of this invention is a kind of nutritious algae liquid and preparation method thereof, but in preparation process, relate to acid treatment and processes such as neutralization subsequently, the acid treatment meeting causes the destruction of part nutritional labeling, and removing the inorganic or organic salt that is produced that neutralizes simultaneously also has certain degree of difficulty.2, the extracting method of water-soluble spirulina polyose, classification number: CO8B37/04, the applying date: 94.05.16, the day for announcing: 95.11.22, application number: 94105389, applicant: South China Normal University.This invention is a kind of extracting method of water-soluble spirulina polyose, specifically adopts the aqueous slkali extracting, neutralizes with organic acid.This method only relates to the preparation of spirulina-active polysaccharide, underuses other composition of spirulina, and this method also relates to acid, alkali treatment simultaneously.3, from spirulina, extract the method and the application thereof of protein and polysaccharide, classification number: CO7K1/14, the applying date: 94.08.24, the day for announcing: 95.08.09, application number: 94112454, applicant: Shenyang Institute of Phamaceutical Industry.This invention relates to from spirulina the method for extracting protein and polysaccharide, specifically be in the time of 5-15 ℃, to soak to extract 5-20 hour, triplicate, through further extract, method such as precipitation makes protein and polysaccharide.Because nutritious spirulina is abundant, this method is 5-15 ℃ of long soaking time, and is perishable; This method is extracted being difficult for dissolved substances at low temperatures simultaneously, and the nutritional labeling of spirulina is also underused.
China's non-patent literature: 1, the purification of spirulina antiradiation polysaccharide and analysis, Pang Qishen, Guo Baojiang, Ruan Jihong, Acta Biochimica et Biophysica Sinica, 1989,21:445-449.The document has been reported the method for extracting the water-soluble polysaccharide with radioresistance activity from spirulina.2, the research of the separation of spirulina maxim polysaccharide, purifying and anti-oxidation characteristics thereof, Zhou Zhigang, Liu Zhili, Liu Xuexian, Botany Gazette, 1997,39:77-81.Document report spirulina polysaccharide has the effect of significant removing hydroxy radical.More than 2 pieces of documents just study at the preparation and the biologically active of spirulina polysaccharide, the nutritional labeling of spirulina is not carried out full use.
The objective of the invention is to from spirulina, extract the nutritional labeling of multiple maintenance natural activity, improve the utilization ratio of spirulina, use for the dissimilar beverage of preparation, oral liquid or cosmetic additive agent.
For the purpose that realizes inventing, its technical scheme is to be material with the spirulina, create the method that substep extracts, first step freeze thawing extracts algocyan, second step combination enzymolysis and extraction free amino acid, oligopeptides and oligosaccharides, the 3rd step hot water extracting active polysaccharide, protein, thus make full use of spirulina and improve utilization ratio.Implementing beneficial effect of the present invention mainly contains:
1, owing to taked effective substep extractive technique, makes that the nutritional labeling in the spirulina is extracted fully, and kept the color of coloring matter.
2, according to back product working properties difference, each goes on foot extract and both can use respectively, can be used again.
When 3, extracting, use different enzyme combination enzymolysis, both improved extraction effect, make protein fully be converted into free amino acid and oligopeptides again with enzymatic isolation method.
4, in the leaching process without bronsted lowry acids and bases bronsted lowry, do not add any organic solvent yet, do not need last handling processes such as neutralization, thereby in not having and the time various salinities problem such as residual, both kept natural activity, free from environmental pollution again.
5, technology is simple, and easy operating can be used for suitability for industrialized production.
Embodiment:
1, freeze thawing is extracted.Get spirulina powder and soak in distilled water that (algae: water is 1: 1, W/W; Spend the night under the room temperature) after, multigelation 2-4 time thaws, and is centrifugal, gets supernatant, obtains pigment nutritional labelings such as algocyan.
2, combination enzymolysis and extraction.Algae-residue adding distil water 5-10 after the freeze thawing doubly adds lysozyme and pectase hydrolysis 1-2 hour, adds amylase, papain, pepsin, each hydrolysis 0.5-1 of trypsase hour more respectively.Hydrolysis temperature is 45 ± 2 ℃ except that papain, and other is 37 ± 2 ℃.Institute's enzyme concentration is different different because of enzyme unit alive, when stirring evenly the back enzymolysis as add 10 liters of distilled water with 1 kilogram of spirulina powder, add lysozyme 1 gram of food industry, pectase 4 grams, each 2 gram of amylase, papain, pepsin and trypsase with enzyme.Centrifugal behind the enzymolysis, get supernatant, obtain nutritional labelings such as free amino acid, oligopeptides, oligosaccharides.
3, hot water extracting.Algae-residue adding distil water 5-10 behind the enzymolysis doubly stirs 2 times (being respectively 2 hours and 1 hour) of extraction at 80 ℃, and centrifugal merging supernatant obtains nutritional labelings such as active polysaccharide, protein.
Each step of gained of the present invention is extracted nutrient solution, can be made into dissimilar health care drinks; Make the auxotype oral liquid of materials such as being rich in amino acid, oligopeptides, oligosaccharides, active polysaccharide; Also can respectively color table materials such as algocyan be made the cosmetics with different health cares with the active polysaccharide with radioresistance, anti-oxidation characteristics.
Claims (3)
1, from spirulina, extract the extracting method of a kind of nutritive component of spirulina of algocyan, free amino acid, oligopeptides, active polysaccharide and protein, it is characterized in that carrying out freeze thawing, combination enzymolysis and hot water substep extraction method:
A. freeze thawing is extracted.Get spirulina powder in distilled water, soak (algae: water is 1: 1, W/W) spend the night under the room temperature, multigelation 2-4 time, the centrifuging and taking supernatant obtains algocyan;
B. make up enzymolysis and extraction.Algae-residue adding distil water 5-10 after the freeze thawing doubly adds lysozyme and pectase hydrolysis 1-2 hour, adds amylase, papain, pepsin, each hydrolysis 0.5-1 of trypsase hour more respectively, and is centrifugal, gets supernatant, obtains free amino acid, oligopeptides, oligosaccharides;
C. hot water extracting.Algae-residue adding distil water 5-10 behind the enzymolysis doubly stirs extraction 2 times at 80 ℃, and centrifugal merging supernatant obtains active polysaccharide, protein.
2, the extracting method of nutritive component of spirulina according to claim 1, the hydrolysis temperature that it is characterized in that papain is 45 ± 2 ℃, lysozyme and pectase, amylase, pepsin, tryptic hydrolysis temperature are 37 ± 2 ℃.
3, the extracting method of nutritive component of spirulina according to claim 1, the consumption that it is characterized in that enzyme when 1 kilogram of spirulina powder of enzymolysis, adds lysozyme 1 gram of food industry with enzyme, pectase 4 grams, each 2 gram of amylase, papain, pepsin and trypsase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97109320A CN1119946C (en) | 1997-11-27 | 1997-11-27 | Extracting method for nutritive component of spirulina |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97109320A CN1119946C (en) | 1997-11-27 | 1997-11-27 | Extracting method for nutritive component of spirulina |
Publications (2)
| Publication Number | Publication Date |
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| CN1218636A true CN1218636A (en) | 1999-06-09 |
| CN1119946C CN1119946C (en) | 2003-09-03 |
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| CN97109320A Expired - Fee Related CN1119946C (en) | 1997-11-27 | 1997-11-27 | Extracting method for nutritive component of spirulina |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1098707C (en) * | 1999-07-19 | 2003-01-15 | 齐清 | Medicinal composition containing algal protein polyose extract and extraction of algal protein polyose |
| CN100383183C (en) * | 2004-11-30 | 2008-04-23 | 台盐实业股份有限公司 | Algae ferment hydrolysate and its producing method |
| CN100434528C (en) * | 2005-03-24 | 2008-11-19 | 中国科学院植物研究所 | Simplified method for extracting a great lot phycocyanin |
| CN101348754B (en) * | 2007-07-16 | 2012-05-30 | 青岛啤酒股份有限公司 | Spirulina extract and preparation thereof |
| CN102987408A (en) * | 2012-10-17 | 2013-03-27 | 徐州鸿宇农业科技有限公司 | Method for extracting nutritional ingredients such as free amino acids from waste cordyceps militaris cocoons |
| CN103013726A (en) * | 2012-12-12 | 2013-04-03 | 赵波 | Spirulina blue polysaccharide green bear, liquor and beverage and preparation method |
| CN103082160A (en) * | 2013-01-15 | 2013-05-08 | 浙江现代中药与天然药物研究院有限公司 | High-efficiency deodorization and low-nutritional content loss method of conducting wall breaking and deodorization to spirulina |
| CN104644694A (en) * | 2015-02-09 | 2015-05-27 | 江苏海王健康生物科技有限公司 | Preparation method of ultramicro spirulina powder as well as preparation and application thereof |
| CN105125668A (en) * | 2015-07-28 | 2015-12-09 | 常州工程职业技术学院 | Compound lotus leaf health product and preparation method thereof |
| CN106562273A (en) * | 2016-10-27 | 2017-04-19 | 张文艳 | Method for extracting cyanophycin substances from spirulina |
| CN107475120A (en) * | 2016-06-07 | 2017-12-15 | 张德荣 | A kind of preparation method of broken wall spirulina |
| CN111909811A (en) * | 2020-07-27 | 2020-11-10 | 深圳市太空微藻生物科技有限公司 | Preparation method and product of algae-flavor liquor |
-
1997
- 1997-11-27 CN CN97109320A patent/CN1119946C/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1098707C (en) * | 1999-07-19 | 2003-01-15 | 齐清 | Medicinal composition containing algal protein polyose extract and extraction of algal protein polyose |
| CN100383183C (en) * | 2004-11-30 | 2008-04-23 | 台盐实业股份有限公司 | Algae ferment hydrolysate and its producing method |
| CN100434528C (en) * | 2005-03-24 | 2008-11-19 | 中国科学院植物研究所 | Simplified method for extracting a great lot phycocyanin |
| CN101348754B (en) * | 2007-07-16 | 2012-05-30 | 青岛啤酒股份有限公司 | Spirulina extract and preparation thereof |
| CN102987408A (en) * | 2012-10-17 | 2013-03-27 | 徐州鸿宇农业科技有限公司 | Method for extracting nutritional ingredients such as free amino acids from waste cordyceps militaris cocoons |
| CN102987408B (en) * | 2012-10-17 | 2014-01-15 | 徐州鸿宇农业科技有限公司 | Method for extracting nutritional ingredients such as free amino acids from waste cordyceps militaris cocoons |
| CN103013726B (en) * | 2012-12-12 | 2017-07-11 | 赵波 | The algae indigo plant green beer of polysaccharide and drinks and beverage and preparation method thereof |
| CN103013726A (en) * | 2012-12-12 | 2013-04-03 | 赵波 | Spirulina blue polysaccharide green bear, liquor and beverage and preparation method |
| CN103082160A (en) * | 2013-01-15 | 2013-05-08 | 浙江现代中药与天然药物研究院有限公司 | High-efficiency deodorization and low-nutritional content loss method of conducting wall breaking and deodorization to spirulina |
| CN103082160B (en) * | 2013-01-15 | 2019-03-01 | 浙江现代中药与天然药物研究院有限公司 | A kind of efficient deodorant, low nutrition ingredients from lossing breaking wall of spirullina princeps fishy-removing-method |
| CN104644694A (en) * | 2015-02-09 | 2015-05-27 | 江苏海王健康生物科技有限公司 | Preparation method of ultramicro spirulina powder as well as preparation and application thereof |
| CN105125668A (en) * | 2015-07-28 | 2015-12-09 | 常州工程职业技术学院 | Compound lotus leaf health product and preparation method thereof |
| CN107475120A (en) * | 2016-06-07 | 2017-12-15 | 张德荣 | A kind of preparation method of broken wall spirulina |
| CN106562273A (en) * | 2016-10-27 | 2017-04-19 | 张文艳 | Method for extracting cyanophycin substances from spirulina |
| CN111909811A (en) * | 2020-07-27 | 2020-11-10 | 深圳市太空微藻生物科技有限公司 | Preparation method and product of algae-flavor liquor |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1119946C (en) | 2003-09-03 |
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Granted publication date: 20030903 Termination date: 20091228 |