CN113559023A - Anhydrous yeast essence filtrate and preparation method thereof - Google Patents
Anhydrous yeast essence filtrate and preparation method thereof Download PDFInfo
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The invention provides anhydrous yeast essence filtrate which comprises a schizosaccharomyces cerevisiae lysate, a yeast fermentation product filtrate extract, a yeast extract, hydrolat and glycerol. The anhydrous yeast essence filtrate has high skin absorption rate, improves skin water content, and has good wrinkle removing effect.
Description
Technical Field
The invention relates to anhydrous yeast essence filtrate and a preparation method thereof, belonging to the technical field of cosmetics.
Background
The yeast lysate is obtained by culturing, inactivating and decomposing Bifidobacterium to obtain metabolite, cytoplasmic fraction, cell wall component and polysaccharide complex. It has strong immunosuppressive activity and can promote DNA repair, effectively protect skin from damage caused by ultraviolet rays, and is used for skin care, sun protection and after-sun care products of emulsion, water-based and hydroalcoholic systems, and helps to prevent photoaging of epidermis and dermis.
The extract of the filtrate of the yeast fermentation product is a product extracted from secretion by removing yeast after the yeast is fermented. The skin conditioner is rich in natural active ingredient substances such as small molecular amino acids, polypeptides, nucleotides, vitamins and the like, and is a very good comprehensive skin conditioner.
The yeast extract is light yellow powder which is prepared by breaking the walls of yeast, extracting protein, nucleic acid, vitamin and the like, and carrying out biological enzymolysis on the extract and is rich in natural active ingredients such as micromolecule amino acid, peptide, nucleotide, vitamin and the like. The cosmetic has amino acid content of above 30%, total protein content of above 50%, and nucleotide content of above 10%, and has effects of keeping moisture, activating, improving free radical resistance of skin, recovering elasticity of aged epidermis, and delaying skin aging.
However, the common schizosaccharomyces cerevisiae lysate, the extract of the filtrate of the yeast fermentation product or the yeast extract has insufficient skin absorption rate, so that the skin care effect is not ideal and troubles are brought to users.
Disclosure of Invention
The invention provides anhydrous yeast essence filtrate and a preparation method thereof, which can effectively solve the problems.
The invention is realized by the following steps:
an anhydrous yeast essence filtrate comprises cracked yeast lysate, yeast fermentation product filtrate extract, yeast extract, hydrolat and glycerol.
As a further improvement, the mass-to-volume ratio of the yeast lysate, the yeast fermentation product filtrate extract, the yeast extract, the hydrolat and the glycerol is 0.5-5 mg: 0.5-5 mg: 0.5-5 mg: 15-25 mL: 1.5-2.5 mL.
As a further improvement, the mass to volume ratio of the yeast lysate, yeast fermentation product filtrate extract, yeast extract, hydrolat and glycerol is 2 mg: 2 mg: 2 mg: 18mL of: 2 mL.
As a further improvement, the hydrosol is rose hydrosol.
A preparation method of anhydrous yeast essence filtrate comprises the following steps:
s1, mixing the hydrolat and the glycerol uniformly to obtain hydrolat mixed liquid;
s2, mixing the lysate of the yeast schizolysis, the extract of the filtrate of the yeast fermentation product, the yeast extract and the hydrolat mixed solution, stirring uniformly, sterilizing and refrigerating.
As a further improvement, the mass-to-volume ratio of the yeast lysate, the yeast fermentation product filtrate extract, the yeast extract, the hydrolat and the glycerol is 0.5-5 mg: 0.5-5 mg: 0.5-5 mg: 15-25 mL: 1.5-2.5 mL.
As a further improvement, the mass to volume ratio of the yeast lysate, yeast fermentation product filtrate extract, yeast extract, hydrolat and glycerol is 2 mg: 2 mg: 2 mg: 18mL of: 2 mL.
As a further improvement, the hydrosol is rose hydrosol.
An application of the anhydrous yeast essence filtrate in preparing skin care products is provided.
The invention has the beneficial effects that:
the anhydrous yeast essence filtrate contains the cracked yeast fermentation lysate, the yeast extract and the yeast fermentation product filtrate extract, is dissolved by using the pure dew to dissolve various active substances in the filtrate, and meanwhile, the solution is in a micro-emulsion form, so that the absorption efficiency of the skin on the nutrient substances can be improved, the active substances are mutually matched, the moisture content of the skin is increased, the skin dryness is improved, and the anhydrous yeast essence filtrate has a good effect of removing wrinkles.
The anhydrous yeast essence filtrate contains a yeast fermentation lysate, a yeast extract and a yeast fermentation product filtrate extract, wherein various active ingredients have various and safe action paths and do not have adverse reaction.
The anhydrous yeast essence filtrate adopts rose hydrosol, can effectively dissolve various active ingredients in the yeast fermentation lysate, the yeast extract and the yeast fermentation product filtrate extract, and the various active ingredients play a synergistic role, so that the moisture content of skin is effectively improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a graph showing the rate of increase in skin moisture content provided in test example 1 of the present invention.
Fig. 2 is a graph showing wrinkle removal efficiency provided in test example 2 of the present invention.
Fig. 3 is a graph showing the change with time of skin moisturizing provided in test example 4 of the present invention.
Fig. 4 is a graph of the change in skin moisture content provided in example 2 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Example 1
1. Preparation of fermentation lysate of yeast schizosaccharomyces
(1) Inoculating Bifidobacterium longum into culture medium, performing anaerobic fermentation at 10% (V: V) and initial pH of 6.6-7.0, and standing at 37-40 deg.C for 13 hr. The fermentation culture medium comprises the following components in percentage by mass/volume: 1.5% of yeast powder, 2.0% of glucose, 1.5% of casein peptone, 1.0% of beef peptone, 1.5% of hydrolyzed milk protein, 0.5% of anhydrous sodium acetate, 0.2% of triammonium citrate, 800.1% of tween-tween, 0.05% of magnesium sulfate, 0.02% of manganese sulfate and 0.05% of dipotassium hydrogen phosphate.
(2) After the culture is finished, the bacterial liquid is slightly shaken up, and the cell wall is broken under the condition of low temperature by adopting an ultrasonic breaking method, and the ultrasonic treatment is carried out for 5-6 s/time and the circulation is carried out for 15 times.
(3) After the crushing is finished, adding a small amount of suspending agent, slightly sucking and beating by using a liquid transfer gun to ensure that the suspending agent is completely fused, centrifuging at high speed and low temperature (-20 ℃), wherein the rotating speed is 12000r/min, and removing supernate to keep precipitate substances.
(4) And (4) taking the precipitate, and performing low-temperature freeze drying to prepare the yeast schizolysis product.
2. Preparation of extract from yeast fermentation product filtrate
The culture medium is 26.7ml of 85% phosphoric acid, 20g/L of peptone, 18.2g/L of potassium sulfate, 14.9g/L of magnesium sulfate heptahydrate, 0.93g/L of calcium sulfate dihydrate and 4.13g/L of potassium hydroxide; adjusting pH to 5.5 with 25% ammonia water, inoculating yeast with 10% inoculum size. Culturing at 21 deg.C for 24 hr, and fermenting for 3 days to obtain fermented product; homogenizing and centrifuging the fermentation product, and filtering supernatant to obtain yeast fermentation product filtrate. And after ultrafiltration and concentration, freeze-drying the yeast fermentation product filtrate to obtain the yeast fermentation product filtrate extract.
3. Preparation of Yeast extract
And (3) homogenizing and centrifuging the fermentation product to obtain thalli, repeatedly washing the thalli with deionized water, centrifuging to remove supernatant, and repeatedly washing for 3 times to obtain the thalli. And (3) ultrasonically crushing the saccharomycetes by adopting a low-temperature ultrasonic crushing method, centrifuging after crushing, taking supernate, and repeatedly crushing for three times. Collecting supernatant, ultrafiltering, concentrating, and lyophilizing at low temperature to obtain yeast extract.
4. Preparing a hydrolat mixed solution: measuring 180ml of rose hydrosol by using a measuring cylinder, mixing with 20ml of glycerin, continuously stirring until the mixture is completely fused, and placing the mixture in a refrigerator at 4 ℃ for cooling for later use.
5. Preparation of substance S8: accurately weighing the secondary fission yeast fermentation lysate, the yeast fermentation product filtrate extract and the yeast extract, respectively weighing 2.0mg, sequentially adding into 20ml of the hydrolat mixed solution, slightly stirring until the mixture is uniformly mixed, sterilizing, and then placing in a refrigerator at 4 ℃ for standing and refrigerating for 12 h.
Comparative example 1
Preparation of substance S1: accurately weighing 2.0mg of the prepared yeast schizosaccharomyces cerevisiae fermentation lysate, adding into 20ml of the hydrolat mixed solution, slightly stirring until the mixture is uniformly mixed, sterilizing, and then placing in a refrigerator at 4 ℃ for standing and refrigerating for 12 h. The preparation of the hydrosol mixture solution was the same as in example 1.
Comparative example 2
Preparing a glycerol mixed solution: 180ml of deionized water is weighed by a measuring cylinder and mixed with 20ml of glycerin, the mixture is continuously stirred until the mixture is completely fused, and the mixture is placed in a refrigerator at 4 ℃ for cooling for standby.
Preparation of substance S2: accurately weighing 2.0mg of the prepared yeast schizosaccharomyces cerevisiae fermentation lysate, adding into 20ml of glycerol mixed solution, slightly stirring until the mixture is uniformly mixed, sterilizing, and then placing in a refrigerator at 4 ℃ for standing and refrigerating for 12 h.
Comparative example 3
Preparation of substance S3: accurately weighing 2.0mg of yeast fermentation product filtrate extract, adding into 20ml of the hydrolat mixed solution, slightly stirring to mix well, sterilizing, and standing and refrigerating in a refrigerator at 4 deg.C for 12 h. Wherein, the preparation of the mixed solution of the yeast fermentation product filtrate extract and the hydrolat is the same as that of example 1.
Comparative example 4
Preparation of substance S4: accurately weighing 2.0mg of yeast fermentation product filtrate extract, adding into 20ml of glycerol mixed solution, slightly stirring to mix well, sterilizing, and standing and refrigerating in refrigerator at 4 deg.C for 12 hr. Wherein, the preparation of the extract of the yeast fermentation product filtrate is the same as that of example 1, and the preparation of the glycerol mixture is the same as that of comparative example 2.
Comparative example 5
Preparation of substance S5: accurately weighing yeast extract 2.0mg, adding into the hydrolat mixed solution (20ml), stirring slightly until mixing well, sterilizing, and refrigerating at 4 deg.C for 12 hr. The preparation of the mixed solution of yeast extract and hydrolat was the same as in example 1.
Comparative example 6
Preparation of substance S6: accurately weighing yeast extract 2.0mg, adding into glycerol mixture 20ml, stirring slightly until mixing well, sterilizing, and refrigerating in refrigerator at 4 deg.C for 12 hr. The yeast extract was prepared in the same manner as in example 1, and the glycerin mixture was prepared in the same manner as in comparative example 2.
Comparative example 7
Preparation of substance S7: accurately weighing 2.0mg of each of the yeast fermentation lysate, the yeast fermentation product filtrate extract and the yeast extract, sequentially adding into 20ml of the glycerol mixed solution, slightly stirring until the mixture is uniformly mixed, sterilizing, and standing and refrigerating in a refrigerator at 4 ℃ for 12 h. Wherein, the yeast fermentation lysate, the yeast fermentation product filtrate extract and the yeast extract were prepared in the same manner as in example 1, and the glycerol mixture was prepared in the same manner as in comparative example 2.
Test example 1 hydration degree and skin elasticity of skin
160 subjects of 20-40 years old age were randomly selected, 80 for male and female, respectively, and randomly assigned to 8 groups. Substances S1-S8 were used, respectively. After cleaning the face in the morning and evening every day, 2 drops of the liquid medicine are used, the liquid medicine is massaged and absorbed, the experimental group is smeared on the left face, and the deionized water with the same amount is smeared on the right face serving as a blank control group. And testing the water content of the face skin position of the volunteer by using a skin moisture tester every day, selecting five positions on the same face position in each test, calculating the average value of the water content, and calculating the content increase rate. The calculation formula is as follows: the skin moisture content increase rate is (test group moisture content-blank group moisture content)/blank group moisture content x 100%. The test was performed once a day for 30 days. The test results are shown in fig. 1.
From this, it was found that the moisture content of the skin increased after 30 days of the use of the substance S1-S8 solution, but the increase rate of the substance S8 was the greatest. The increase rate of the group S8 is significantly higher than that of the group S7, which indicates that the hydrolat is adopted to dissolve the schizosaccharomyces cerevisiae fermentation lysate, the yeast extract and the yeast fermentation product filtrate extract, and more active substances can be dissolved than glycerin, so that the better water retention effect is exerted. The increasing rate of the S8 group is higher than the sum of the S1, the S3 and the S5 group, which shows that the extracts of the schizosaccharomyces cerevisiae fermentation lysate, the yeast extract and the yeast fermentation product filtrate extract are matched with each other, so that the synergistic effect is exerted, and the moisture content of the skin is improved together.
Test example 2 wrinkle removal and Fine line leveling effects
160 subjects of 20-40 years old age were randomly selected, 80 for male and female, respectively, and randomly assigned to 8 groups. Substances S1-S8 were used, respectively. After cleaning the face in the morning and evening every day, 2 drops of the liquid medicine are used, the liquid medicine is massaged and absorbed, the experimental group is smeared on the left face, and the deionized water with the same amount is smeared on the right face serving as a blank control group. And (3) calculating the wrinkle removal efficiency by using a skin color difference tester as a testing instrument and testing the wrinkles of the face. The test was performed once a day for 60 days. The test results are shown in fig. 2.
From this, it was found that the removal efficiency of wrinkles on the skin was increased after 60 days using the solutions of the substances S1 to S8, but the increase rate of the substance S8 was the greatest. The fine lines declined by nearly 60% at 60 days with the S8 solution, with a marked improvement in wrinkle depth. The increase rate of the group S8 was significantly higher than that of the group S7, indicating that the use of hydrolat to dissolve the yeast lysate, yeast extract and yeast fermentation product filtrate extract resulted in the dissolution of more active substances than glycerol, thereby exerting a better wrinkle removal effect. The increasing rate of the S8 group is higher than the sum of the S1, the S3 and the S5 group, which shows that the extracts of the schizosaccharomyces cerevisiae fermentation lysate, the yeast extract and the yeast fermentation product filtrate extract are matched with each other, so that the synergistic effect is exerted, and the wrinkle removal rate is improved together.
Test example 3 skin improvement
In order to further observe the improvement of the substance S8 on the skin of the female before and after makeup, 30 women aged 25-30 and often made up are selected to test the mixed solution, 2 drops of the product are used every day after makeup removal, the mixed solution is absorbed by massage, and after 30 days, the skin is found to be remarkably improved, wherein the moisture content of the skin is improved by 20%, and the fine wrinkles removal rate is over 50%.
Test example 4 graph showing change of skin moisturizing with time
30 subjects of 20-40 years old were randomly selected. Substance S8 was used. After cleaning the face in the morning and evening every day, 2 drops of the liquid medicine are used, the liquid medicine is massaged and absorbed, the experimental group is smeared on the left face, and the deionized water with the same amount is smeared on the right face serving as a blank control group. And testing the water content of the face skin position of the volunteer by using a skin moisture tester at intervals, selecting five positions on the same face position in each test, testing once a day, obtaining the average value of the water content, and calculating the content increase rate. The calculation formula is as follows: the skin moisture content increase rate is (test group moisture content-blank group moisture content)/blank group moisture content x 100%. The test was carried out for 72 days. The test results are shown in fig. 3.
Therefore, after the product is used, the skin moisture content increasing rate is continuously increased and maintained for about 60 days, and after the product is continuously used for 60 days, the skin moisture content increasing rate is maintained at a stable level.
Example 2
In order to investigate the suitable use concentrations of the yeast secondary fission lysate, yeast extract, yeast fermentation product filtrate extract, we performed the investigation using the controlled variable method. 180 subjects of 20-40 years old were randomly selected, wherein the number of the male and female subjects was 90, and the subjects were randomly and evenly divided into three groups, i.e., a two-split yeast fermentation lysate test group, a yeast extract test group, and a yeast fermentation product filtrate extract test group, wherein each group was randomly and evenly divided into 6 groups.
In the yeast extract experimental group, the concentration of yeast extract was set as 6 concentration gradients with variable set to 0, 1, 2, 3, 4, 5mg/ml, wherein the addition amounts of yeast fermentation product filtrate extract and yeast schizolysis product extract were set to 0.2mg/ml, respectively.
In the yeast fermentation product filtrate extract experimental group, the concentration of the yeast fermentation product filtrate extract is set as a variable and is set as 0, 1, 2, 3, 4 and 5mg/ml concentration gradients, wherein the addition amounts of the yeast extract and the schizosaccharomyces cerevisiae lysate extract are respectively set as 0.2 mg/ml.
In the experimental group of the split yeast fermentation lysate, the variables of the split yeast fermentation lysate are set as 0, 1, 2, 3, 4 and 5mg/ml concentration gradients, wherein the addition amounts of the yeast fermentation product filtrate extract and the yeast extract are respectively set as 0.2 mg/ml.
After cleaning the face in the morning and evening every day, 2 drops of the product was applied, massaged and absorbed, and the left face was smeared with the experimental group and the right face was smeared with the same amount of deionized water as a blank control group. And testing the water content of the face skin position of the volunteer by using a skin moisture tester at intervals, selecting five positions on the same face position in each test, testing once a day, calculating the average value of the water content after 7 days of testing, and calculating the content increase rate. The calculation formula is as follows: the skin moisture content increase rate is (test group moisture content-blank group moisture content)/blank group moisture content x 100%. The test results are shown in fig. 4.
It is clear from this that the effect is relatively stable when the concentration of the yeast lysate, yeast extract, yeast fermentation filtrate extract is 1 mg/ml.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. An anhydrous yeast essence filtrate, which is characterized by comprising a yeast schizolysis product, a yeast fermentation product filtrate extract, a yeast extract, hydrolat and glycerol.
2. The anhydrous yeast essence filtrate according to claim 1, wherein the mass-to-volume ratio of the yeast lysate, the yeast fermentation product filtrate extract, the yeast extract, the hydrolat and the glycerol is 0.5-5 mg: 0.5-5 mg: 0.5-5 mg: 15-25 mL: 1.5-2.5 mL.
3. The anhydrous yeast essence filtrate according to claim 1, wherein the mass-to-volume ratio of the yeast lysate, the yeast fermentation product filtrate extract, the yeast extract, the hydrolat and the glycerol is 2 mg: 2 mg: 2 mg: 18mL of: 2 mL.
4. The anhydrous yeast essence filtrate of claim 1, wherein the hydrolat is rose hydrolat.
5. The preparation method of the anhydrous yeast essence filtrate is characterized by comprising the following steps of:
s1, mixing the hydrolat and the glycerol uniformly to obtain hydrolat mixed liquid;
s2, mixing the lysate of the yeast schizolysis, the extract of the filtrate of the yeast fermentation product, the yeast extract and the hydrolat mixed solution, stirring uniformly, sterilizing and refrigerating.
6. The method for preparing anhydrous yeast essence filtrate according to claim 5, wherein the mass-to-volume ratio of the yeast lysate, the yeast fermentation product filtrate extract, the yeast extract, the hydrolat and the glycerol is 0.5-5 mg: 0.5-5 mg: 0.5-5 mg: 15-25 mL: 1.5-2.5 mL.
7. The method for preparing anhydrous yeast essence filtrate according to claim 5, wherein the mass-to-volume ratio of the yeast lysate, the yeast fermentation product filtrate extract, the yeast extract, the hydrolat and the glycerol is 2 mg: 2 mg: 2 mg: 18mL of: 2 mL.
8. The method for preparing anhydrous yeast essence filtrate according to claim 5, wherein the hydrosol is rose hydrosol.
9. Use of the anhydrous yeast essence filtrate according to any one of claims 1 to 4 for the preparation of a skin care product.
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| CN115581655A (en) * | 2022-04-13 | 2023-01-10 | 广州市万千粉丝化妆品有限公司 | Application and preparation of 3X nutrient and muscle energy maintenance interstitial fluid preparation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112107511A (en) * | 2020-07-31 | 2020-12-22 | 广州优科生物科技有限公司 | Preparation method and application of schizosaccharomyces cerevisiae fermentation lysate |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115581655A (en) * | 2022-04-13 | 2023-01-10 | 广州市万千粉丝化妆品有限公司 | Application and preparation of 3X nutrient and muscle energy maintenance interstitial fluid preparation |
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