CN100381561C - Pseudomonas sp. FK916 producing decomposition enzyme of organic matter and method for decomposition of organic waste using the same - Google Patents
Pseudomonas sp. FK916 producing decomposition enzyme of organic matter and method for decomposition of organic waste using the same Download PDFInfo
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- CN100381561C CN100381561C CNB03803235XA CN03803235A CN100381561C CN 100381561 C CN100381561 C CN 100381561C CN B03803235X A CNB03803235X A CN B03803235XA CN 03803235 A CN03803235 A CN 03803235A CN 100381561 C CN100381561 C CN 100381561C
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C02F2003/001—Biological treatment of water, waste water, or sewage using granular carriers or supports for the microorganisms
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
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- C12R2001/38—Pseudomonas
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Abstract
The present invention relates to Pseudomonas sp. FK 916 producing decomposition enzyme of organic compounds and a method for decomposition of organic waste using the same. The Pseudomonas sp. FK 916 strain which is derived from sewage has an excellent ability to degrade and remove organic matter and carries out the decomposition at a medium temperature between 15 and 35 DEG C. Therefore, it does not emit bad smell and can be effectively used in treatment of organic waste from waste food and factories.
Description
Technical field
The present invention relates to produce pseudomonas (Pseudomonas) FK 916 of organic compound lytic enzyme and handle the method for organic waste with it.The invention particularly relates to the pseudomonas FK 916 that comes from sewage, it has splendid decomposition and removes organic ability in food garbage and the organic waste, and can handle food garbage effectively and can not discharge stink, also relate to and a kind ofly handle the method for organic waste with it.
Background technology
In Korea S, the food garbage that produce every day is 11,577 tons (benchmark in 1999), and it accounts for 26.5% in the solid refuse that the city produces.In these solid refuses, 64% from family, and 36% comes freely, and dining room, collective supply catering industry such as meals place.58.8% buried in the food garbage that is produced, 7.3% burned, 33.9% be recycled again and utilize.Therefore present processing food garbage mainly relies on buries.Yet Seoul National Capital Area will be forbidden directly burying food garbage in the place of burying in city from January, 2005.The serious problems that most regional autonomous bodies face be in food garbage is handled because of burying the environmental pollution that leach liquor caused in the thing, and live in and bury generally being discontented with that the place surrounding resident increases day by day.Simultaneously, with incendive garbage relatively, burning disposal needs more expense, this is because food garbage water content height, therefore is difficult for building burning factory and implementing the place because of resident's opposition is difficult to preserve.Therefore be badly in need of the food garbage treatment process of a kind of economy of exploitation, it can directly use microorganism to decompose, remove or reduce the quantity of food garbage by produce ground at food garbage, thereby increases the cycling and reutilization rate.
Because of the inconsistent characteristic of food garbage and form and water and salt content height, so food garbage is more difficult.In addition, also should take different treatment processs according to different areas and commercial location.Food garbage processing in present research the and cycling and reutilization method comprise that compost treatment, fodder production, anaerobic degradation, sewage merge processing etc., and wherein compost treatment mainly is divided into the extensive compost treatment of processing use on a large scale and the small-scale compost treatment of family and small shop's use.
Compost treatment equipment comprises fermenter and moisture eliminator on a small scale, and is placed on apratment building, public in the buildings such as meals place, hotel, dining room, yet, wherein there are many problems.
Conventional composting arrangement is included in high temperature bottom fermentation food garbage and produces mixed fertilizer and feed, and its matter of utmost importance is because of thermophilic fermentation or divides degraded to give out serious tedious stink that this makes it become unwelcome equipment.In addition, because of Korean's food is the high salt food that contains 1-5% salt amount, be impossible so produce mixed fertilizer with it.Secondly, because of conventional composting arrangement uses actinomyces and thermophile bacteria, they need at high temperature to activate to increase microbial fermentation and rate of decomposition, so equipment should remain on 50-60 ℃, thereby keep the suitable growth temperature of thermophile bacteria, and reduce the fermented product volume and the transpiring moisture of food refuse.Therefore this just needs a large amount of electric power, so that has increased running cost.
For addressing the above problem, the inventor from sewage isolation identification new bacterial strain, it has splendid decomposition and removes organic ability in food garbage and the organic waste, and can under moderate temperature, carry out aerobic to decompose and can not discharge rank smell, finished the present invention thereby utilize this bacterial strain that food garbage is decomposed with degradation treatment.
Summary of the invention
Therefore, the purpose of this invention is to provide a kind of bacterial strain that has splendid decomposition and remove organism ability in food garbage and the organic waste.
Another object of the present invention provides a kind of method of utilizing bacterial strain of the present invention to handle organic waste.
According to the present invention, above-mentioned and other purpose can be finished by pseudomonas FK 916 bacterial strains are provided.
The pseudomonas FK 916 that comes from sewage is in 15-35 ℃, preferred 25-30 ℃ moderate temperature scope, in the presence of sufficient amount of oxygen, decompose and change low-molecular-weight organism into such as organism such as carbohydrate, albumen, fat in can be with food refuse, finally produce stable material such as CO by aerobic
2, H
2O etc., and produce a little heat.Microorganism belongs to pseudomonas simultaneously, can be to NO
3, NO
2, NO carries out denitrogenation and produces N
2, shown in following reaction formula (I).Therefore, pseudomonas FK 916 and other microorganism also can will produce the NH of one of bad smell composition by denitrogenation
3Change N into
2Thereby, remove unpleasant and irritating ammonia flavor.
In addition, the bacterial strain among the present invention it is characterized in that can not producing can discharge bad smell such as materials (table 2) such as indoles, hydrogen sulfide, acetoins.
Pseudomonas FK 916 bacterial strains are deposited in as the Korea S of one of international preservation mechanism on January 7th, 2002 and plant bacterium association (KCCM), and deposit number is KCCM-10350.Except that the bacterial strain of preservation, show some multifarious dissociant in other properties and be also included within the scope of the present invention, as long as they contain and identical 16S RNA sequence, lipid acid composition, form, cultivation and the biochemical character of bacterial strain among the present invention.
In addition, another aspect of the present invention provides the method for utilizing decomposition of pseudomonas FK 916 bacterial strains and removal such as organic wastes such as food garbage.
The feature of this method is that pseudomonas FK 916 bacterial strains or the composition that contains this bacterial strain are joined in the object being treated.This bacterial strain or composition can add by for example the mixture of the nutrient solution of bacterial strain or strain cultured solution and weighting material directly being sprinkled into, and wherein weighting material comprises natural organic matter (as rice shell, sawdust, wood chip), particles of inorganic material (as zeolite or pottery) and plastics.Selectively, bacterial strain is attracted to such as on the weighting material of natural organic matter and particles of inorganic material etc. or on the carrier.Weighting material or carrier can be sprinkling upon on the organic waste or according to known microbe preparation method and operate.
Among the present invention, term " object being treated " refers to the activated sewage mud piece and the sewage rubbish that produce in factory and the organic waste.
Brief Description Of Drawings
From detailed description below in conjunction with accompanying drawing, can more be expressly understood above and other objects of the present invention, feature and advantage, wherein:
Fig. 1 shows test-results, and pseudomonas FK 916 inoculation wherein of the present invention are carried out proteolytic enzyme analysis and protease activity detection in the culture plate that contains nutrient agar;
Fig. 2 shows test-results, and pseudomonas FK 916 inoculation wherein of the present invention are carried out lipase analysis and lipase activity detection in the culture plate that contains nutrient agar;
Fig. 3 is the photo of pseudomonas FK 916; And
Fig. 4 is after showing the nutrient solution that adds pseudomonas FK 916 in food garbage, the graphic representation that food garbage weight reduced with the resolving time.
The preferred plan that invention is implemented
To describe the present invention in detail by following examples now.It should be understood by those skilled in the art that embodiment only is for the present invention is described, but the invention is not restricted to this.
Embodiment 1: the separation of bacterial strain
From the sewage that Jeollabuk-do, Korea (Cholabuk-do) is collected, separate bacterial strain of the present invention on every side.Sewage sample dilutes gradually with physiological saline.A part spreads upon on the LB nutrient agar (contain bacto-tryptone 10g/L, bacterium is used yeast extract 5g/L, NaCl 10g/L, agar 15g/L, pH 7.0), and cultivates down at 25 ℃.Separate the bacterium colony that occurs.
Under 25 ℃, isolating bacterium colony is cultivated in amplification culture medium (LB liquid nutrient medium).Carry out proteolytic enzyme analysis (skimming milk nutrient agar: peptone 5g/L containing on the culture plate of nutrient agar the coating bacterial strain then, bacterium is used yeast extract 3g/L, skimming milk 1g/L, agar 15g/L, pH7.0), the coating bacterial strain carries out lipase analysis (tributyrin nutrient agar: peptone 5g/L on the culture plate of nutrient agar containing, bacterium is used yeast extract 3g/L, tributyrin 5ml/L, agar 15g/L, pH 7.4), the coating bacterial strain carries out amylase analysis (Starch Agar substratum: peptone 5g/L on the culture plate of nutrient agar containing, bacterium is used yeast extract 3g/L, Zulkovsky starch 2g/L, agar 15g/L, pH 7.0), the coating bacterial strain carries out Mierocrystalline cellulose analysis (Mierocrystalline cellulose 1g/L, K on the culture plate of nutrient agar containing
2HPO
41g/L, (NH
4)
2SO
41g/L, MgSO
47H
2O 0.2g/L, CaCl
22H
2O 0.1g/L, FeCl
30.02g/L, Congo red 0.075g/L, agar 15g/L, pH 7.2).Detect in each culture plate the transparent ring that the activity by proteolytic enzyme, lipase, amylase, cellulase forms and identify the separation positive strain.
From result shown in Figure 1 as can be seen, the culture plate that contains nutrient agar (B) of making the culture plate that contains nutrient agar (A) of proteolytic enzyme analysis (A) and doing the lipase analysis forms transparent ring behind inoculation FK 916 bacterial strains.Therefore, point out this bacterial strain can produce proteolytic enzyme and lipase.But, show that also diastatic activity is more weak and do not see cellulase activity.
Embodiment 2: the evaluation of bacterial strain
According to described methods such as Holt (Holt etc., Bergey determinative bacteriology handbook (Bergey, s Manual of Determinative Bacteriology), the 9th edition (1994)), identify in embodiment 1 strain separated by Sherlock system and clustal W.Find that this bacterial strain is the bacterial strain that belongs to pseudomonas, and called after pseudomonas FK 916.This new pseudomonas FK916 is with to contain the Pseudomonas chlororaphis (the false pseudomonas bacillus of chlororaphin) that 80.2% lipid acid forms similar, and with Pseudomonas gessardii CIP 105469 in the similarity that has 99.8% aspect the rRNA.
From photo shown in Figure 3 as can be seen, find that this bacterial strain is the Gram-negative bacteria with bacilliform morphological specificity.Cultural characters is as shown in table 1 below, comprises that suitable growth temperature is 28-30 ℃, tolerance 6%NaCl.Therefore, point out the food garbage of bacterial strain suitable treatment high content of salt of the present invention.
Cultivation and the morphological feature of table 1 pseudomonas FK 916
Method as shown in table 2 by the biochemical characteristic that the API test kit is analyzed, as to describe according to MIDI Com., the result by the gas chromatographic analysis whole-cell fatty acid and utilize the Sherlock system as shown in table 3 to the evaluation of bacterial strain.In addition, according to the method (Verhille etc., International J.Systematic Bacteriol., 49,1559,1999) of descriptions such as Verhille 16s rRNA is separated order-checking.The result shows that bacterial strain contains 1498 bases (Seq.ID.No.:1).Utilize clustal W,, analyze similarity with high degree of specificity microorganism through the gene pool screening by carrying out the rRNA base sequence relatively with other bacterial strain that belongs to pseudomonas.The result is as shown in table 4.The G+C content of analyzing according to De Ley method (De Ley, J., J.Bacteriol., 101,738,1970) is 61.5%.
The biochemical characteristic of table 2 pseudomonas FK 916
Table 3 lipid acid is formed
Table 4 pseudomonas FK 916 and belong between the bacterial strain of pseudomonas 16S rRNA similarity relatively
Embodiment 3: the decomposition of food garbage and removal: food garbage weight is with the reduction of resolving time
Be to analyze the reduction of food garbage weight, pseudomonas FK 916 is inoculated in the LB liquid nutrient medium of sterilization, and at 1 day 1 night of 28 ℃ of following wave and culture.200ml nutrient solution and 100g sawdust (as ventilating and moisture controlled) are joined in the 3L wide-mouth beaker, fully mixing.To add wherein from the 1Kg food garbage (containing 80% moisture approximately) that collect in the dining room of W university, and abundant mixing.After having claimed starting weight, mixture is put into 25 ℃ incubator, stir simultaneously.Weigh mixture 3-4 time every day, and the calculated weight reduced rate.
From result shown in Figure 4 as can be seen, at first day that decomposes, when rubbish weight reduced, food garbage inherent bad smell disappeared, and new not good odour do not occur.After decomposition the 18th day, the weight of rubbish is reduced to 72.3%, illustrates that food garbage is decomposed and removes.The weight reduced rate is rapider with the comparative example 2 that adds LB substratum, sawdust than the comparative example 1 that only adds culture fluid.These presentation of results add sawdust and help the aerobic conditions that provides suitable and can control moisture content, have therefore promoted organic decomposition and removal.In addition, FK 916 bacterial strains suit to carry out under 25 ℃ to organic decomposition and removal effect.Provide air in food garbage zero remover (zero-discharger), to obtain ideal effect continuously but also point out out by stirring.
Comparative example 1: use the decomposition of the food garbage of pseudomonas FK 916 nutrient solutions processing to detect
Pseudomonas FK 916 is inoculated in the LB liquid nutrient medium of sterilization, and at 1 day 1 night of 28 ℃ of following wave and culture.200ml nutrient solution and 100g sawdust (as ventilating and moisture controlled) are joined in the 3L wide-mouth beaker, fully mixing.1Kg food garbage (containing 80% moisture approximately) is added wherein, and abundant mixing.After having claimed starting weight, mixture is put into 25 ℃ incubator, stir simultaneously.Weigh mixture 3-4 time every day, and calculated weight reduced rate (Fig. 4).
Comparative example 2: the decomposition of the food garbage of handling with sawdust detects
200ml is joined in the 3L wide-mouth beaker through the LB liquid nutrient medium and the 100g sawdust (making moisture controlled) of sterilization, fully mixing.1Kg food garbage (containing 80% moisture approximately) is added wherein, and abundant mixing.After having claimed starting weight, mixture is put into 25 ℃ incubator, stir simultaneously.Weigh mixture 3-4 time every day, and calculated weight reduced rate (Fig. 4).
Embodiment 4: the decomposition and the removal of food garbage in zero remover
About 5-10mm), the FK 916 that cultivates according to the described method of embodiment 1 of 5L cultivates the food garbage that bodies, 39Kg collect and join the ﹠amp by Nambang E from the dining room 80Kg wood chip (particle size:; BCo., food garbage zero remover (CLEANGATE-50 type, the capacity: 50kg) of Ltd. production.Operational outfit under 28 ℃ temperature, stirring velocity are 3.2rpm, and ventilation speed is 5.5m
3/ min.After 24 hours, content weight in the weighing-appliance, and calculated weight reduced rate.Decompose beginning after 1 hour, the bad smell of food garbage begins to disappear.After 24 hours, the weight of content is reduced to 76.9%, discharges minimum taste simultaneously.Decompose second day, add the food garbage of 46Kg again, and operational outfit under the same conditions.After 24 hours, the weight reduced rate reaches 90.9%.In 29 days, add food garbage and the operational outfit under the same conditions of 27-60Kg every day.Final accumulative total reduced rate is 94.7%.
Experimental result proves, pseudomonas FK 916 bacterial strains of the present invention can decompose and the food garbage of degrading by once throwing under room temperature and moderate temperature conditions for a long time, thereby are the effective strains of a kind of decomposition and removal such as organic wastes such as food garbage.
Industrial usability
As described in embodiment, because of pseudomonas FK 916 bacterial strains of the present invention under moderate temperature Ability with splendid decomposing food rubbish, and can not discharge bad smell, so it can Economy wholesomely is used for the processing of food garbage, and plays a role in environment-industry.
Sequence?Listing
<110>CHOI，Dong-Seong
FUKAI，Kunihirl
<120>Pseudomonas?sp.FK916?producing?decomposition?enzyme?of?organic?compoundand?method?for?decomposition?of?organic?waste?using?the?same
<130>P03005
<150>KR2002-0007525
<151>2002-02-08
<160>1
<170>KopatentIn?1.71
<210>1
<211>1498
<212>DNA
<213>Pseudomonas?sp.FK916
<400>1
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gcctggtagt?gggggataac?gttcggaaac?ggacgctaat?accgcatacg?tcctacggga <180>
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cactggaact?gagacacggt?ccagactcct?acgggaggca?gcagtgggga?atattggaca <360>
atgggcgaaa?gcctgatcca?gccatgccgc?gtgtgtgaag?aaggtcttcg?gattgtaaag <420>
cactttaagt?tgggaggaag?ggttgtagat?taatactctg?caattttgac?gttaccgaca <480>
gaataagcac?cggctaactc?tgtgccagca?gccgcggtaa?tacagagggt?gcaagcgtta <540>
atcggaatta?ctgggcgtaa?agcgcgcgta?ggtggttagt?taagttggat?gtgaaatccc <600>
cgggctcaac?ctgggaactg?cattcaaaac?tgactgacta?gagtatggta?gagggtggtg <660>
gaatttcctg?tgtagcggtg?aaatgcgtag?atataggaag?gaacaccagt?ggcgaaggcg <720>
accacctgga?ctgatactga?cactgaggtg?cgaaagcgtg?gggagcaaac?aggattagat <780>
accctggtag?tccacgccgt?aaacgatgtc?aactagccgt?tgggagcctt?gagctcttag <840>
tggcgcagct?aacgcattaa?gttgaccgcc?gttttagtac?ggccgcaagg?ttaaaactca <900>
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gggaggacgg?ttaccacggt?gtgattcatg?actggggtga?agtcgtaaca?aggtagcc <1498>
Claims (4)
1. a deposit number that comes from sewage is pseudomonas (Pseudomonas sp.) FK 916 bacterial strains of KCCM-10350, its can be under 15-35 ℃ moderate temperature decomposing food rubbish and can not discharge bad smell.
2. a method of handling organic waste and sewage comprises that adding deposit number as claimed in claim 1 is pseudomonas FK 916 bacterial strains of KCCM-10350.
3. method as claimed in claim 2, wherein said pseudomonas FK 916 bacterial strains mix adding with any material that is selected from natural organic matter, particles of inorganic material and the plastics as weighting material.
4. method as claimed in claim 3, wherein said natural organic matter is selected from a meter shell, sawdust and wood chip, and described particles of inorganic material is selected from zeolite and pottery.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2002-0007525A KR100437103B1 (en) | 2002-02-08 | 2002-02-08 | Pseudomonas sp. FK 916 producing decomposition enzyme of organic compound and method for decomposition of organic waste using the same |
| KR2002/7525 | 2002-02-08 |
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| Publication Number | Publication Date |
|---|---|
| CN1729286A CN1729286A (en) | 2006-02-01 |
| CN100381561C true CN100381561C (en) | 2008-04-16 |
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| Country | Link |
|---|---|
| JP (2) | JP2003235547A (en) |
| KR (1) | KR100437103B1 (en) |
| CN (1) | CN100381561C (en) |
| AU (1) | AU2003206244A1 (en) |
| WO (1) | WO2003066837A1 (en) |
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| JP4947673B2 (en) * | 2009-06-22 | 2012-06-06 | 晋二郎 金澤 | Planted cultivation soil and its manufacturing method |
| JP5308542B2 (en) | 2010-02-05 | 2013-10-09 | 有限会社メイショウ | Degradation method of organic matter using mother cell degrading enzyme accompanying spore formation of microorganisms |
| KR101356747B1 (en) * | 2012-11-08 | 2014-02-03 | 이호식 | Composition for decomposing food waste |
| CN105347639B (en) * | 2015-12-10 | 2018-03-16 | 哈尔滨工业大学 | Microfauna adheres to combined packing |
| CN113462622B (en) * | 2021-09-03 | 2021-11-26 | 佛山市玉凰生态环境科技有限公司 | Pseudomonas for efficiently degrading various aromatic pollutants and application thereof |
| CN114410552B (en) * | 2022-03-28 | 2022-06-17 | 佛山市玉凰生态环境科技有限公司 | Pseudomonas gainsteri for oxidizing sulfur and application of pseudomonas gainsteri in sulfur reduction in black and odorous water body air |
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| CN1308670A (en) * | 1998-01-16 | 2001-08-15 | 第一制糖株式会社 | Microorganisms and preparation for disposing of organic wastewater |
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| CA1164070A (en) * | 1981-06-04 | 1984-03-20 | Georgian Manufacturing Ltd. | Wave condition sensing apparatus |
| US4461834A (en) * | 1983-04-20 | 1984-07-24 | Homestake Mining Company | Strain of Pseudomonas paucimobilis |
| US4863872A (en) * | 1987-11-03 | 1989-09-05 | Wolfram James H | Novel pseudomonas microorganism for biodegradation of liquid scintillation cocktails |
| US5508193A (en) * | 1993-08-31 | 1996-04-16 | Regents Of The University Of Minnesota | Pseudomonas strain for degradation of s-triazines in soil and water |
| JP3402705B2 (en) * | 1993-12-24 | 2003-05-06 | キヤノン株式会社 | How to decompose organic chlorine compounds |
| US5531898A (en) * | 1995-04-06 | 1996-07-02 | International Organic Solutions Corp. | Sewage and contamination remediation and materials for effecting same |
| KR0170603B1 (en) * | 1996-08-09 | 1999-02-01 | 백영배 | Microorganism preparation accelerating decomposition that has excellent organic byproduct resolving power and effluvium removing power and its method for preparation |
| KR100348991B1 (en) * | 1999-12-15 | 2002-08-17 | 박두현 | Process for food waste treatment using the bacteria with proteolytic, starchyolytic and lipolytic activity |
| KR100430031B1 (en) * | 2000-11-30 | 2004-05-03 | 주식회사 이바이오텍 | A processing method and an apparatus for food waste |
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2002
- 2002-02-08 KR KR10-2002-0007525A patent/KR100437103B1/en not_active IP Right Cessation
- 2002-09-17 JP JP2002307630A patent/JP2003235547A/en active Pending
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2003
- 2003-02-08 JP JP2003566188A patent/JP2006501810A/en active Pending
- 2003-02-08 CN CNB03803235XA patent/CN100381561C/en not_active Expired - Fee Related
- 2003-02-08 AU AU2003206244A patent/AU2003206244A1/en not_active Abandoned
- 2003-02-08 WO PCT/KR2003/000277 patent/WO2003066837A1/en active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1308670A (en) * | 1998-01-16 | 2001-08-15 | 第一制糖株式会社 | Microorganisms and preparation for disposing of organic wastewater |
| WO2001004060A2 (en) * | 1999-07-12 | 2001-01-18 | Oy Clewer Ltd | Method of purifying water, suitable bacteria for the method and use thereof |
Non-Patent Citations (1)
| Title |
|---|
| 假单孢杆菌外毒素基因的克隆及用于裸鼠大肠癌基因治疗. 曹广文等人.第二军医大学学报，第19卷第2期. 1998 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2003235547A (en) | 2003-08-26 |
| JP2006501810A (en) | 2006-01-19 |
| KR100437103B1 (en) | 2004-06-23 |
| AU2003206244A1 (en) | 2003-09-02 |
| KR20030067887A (en) | 2003-08-19 |
| WO2003066837A1 (en) | 2003-08-14 |
| CN1729286A (en) | 2006-02-01 |
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