CN110484461A - Dystrophy Bacillus new species and its application - Google Patents
Dystrophy Bacillus new species and its application Download PDFInfo
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Abstract
The invention discloses dystrophy Bacillus new species and its applications.The present invention provides Modestobacter deseti CPCC D00004, are CGMCC No.18004 in the deposit number of China Committee for Culture Collection of Microorganisms's common micro-organisms center.Bacterial strain CPCC D00004 of the present invention has alkaline phosphatase activities and activity of acid phosphatase, it can be with degrading organic phosphor, one plant of functional dystrophy bacillus new species, garbage disposal, soil remediation, control of desert, in terms of will be with a wide range of applications.
Description
Technical field
The present invention relates to microorganism fields, and in particular to dystrophy Bacillus new species and its application.
Background technique
Dystrophy Bacillus (Modestobacter) belongs to bacterium domain (Bacteria)-actinomyces door
(Actinobacteria)-Actinomycetes (Actinobacteria)-Forlan kirschner mesh (Frankiales)-Geodermatophilus section
(Geodermophilaceae)。
2000, the scholars such as Mevs U had found 4 plants of thermophilic cold actinomyces, and number is respectively AA-802, AA-824, AA-825
And AA-826.The 16S rRNA gene sequence information of these bacterial strains shows they and Geodermatophilus (Geodermatophilus)
The similitude of bacterial strain and Mycococcus (Blastococcus) bacterial strain is higher, what is constructed with 16S rRNA gene sequencing
In systematic growth, strains A A-802, AA-824, AA-825 and AA-826 form individual branch, and with Geodermatophilus and
Mycococcus is neighbour.Comprehensive physiological and biochemical property and cytochemistry classification indicators, establish dystrophy Bacillus
(Modestobacter), type sepecies are mostly every dystrophy bacillus (Modestobacter multiseptatus), with strains A A-
826 be type strain.2006, Normand P formally proposed Geodermatophilus section (Geodermophilaceae) this grouping sheet
Member, and over the ground Dermatophilaceae be described, it is specified that Geodermatophilus, Mycococcus and dystrophy Bacillus be under the jurisdiction of it is thermophilic
Skin Cordycepps, Geodermatophilus are type genus (Normand P.Geodermatophilaceae fam.nov., a formal
description.Int J Syst Evol Microbiol.2006Oct；56(Pt 10):2277-8.).
Dystrophy Bacillus includes 9 species altogether at present, greatly mostly from drought desert soil, is also isolated from biological soil knot
The life harsh environments such as skin, salt-tolerant plant rhizosphere, deep-sea bed mud, rock surface.It is reported that dystrophy Bacillus genus strain has anti-spoke
Penetrate, the performances such as drought-resistant and soil protection does not weather, thus be one of desert Environment pioneer microorganism (Martha E,
Trujillo,Michael Goodfellow,Kanungnid Busarakam,Raul Riesco.Modestobacter
lapidis sp.nov.and Modestobacter muralis sp.nov.,isolated from a deteriorated
sandstone historic building in Salamanca,Spain.Antonie van Leeuwenhoek,2015,
108:311-320)。
In recent years, since organic phosphorus in pesticide, chemical fertilizer is enriched in the environment, result in global water quality degenerate problem and
The frequent outburst of toxic wawter bloom.Using microbial degradation as the microorganism remediation technology of the environmental organism pollution administration object of core, tool
There are the remarkable advantages such as efficient, environmentally friendly, economic, by the extensive concern of scholar.Although being separated in habitats at present
More plants of phosphorus-solubilizing bacterias are obtained, but also less to the report of dystrophy Bacillus genus strain；Only one living to dystrophy Bacillus genus strain Soluble phosphorus
Report (Zhang BH, Salam N, Cheng J, et the al.2016.Modestobacter lacusdianchii of property
sp.nov.,a phosphate-solubilizing actinobacterium with ability to promote
microcystis growth microbiology.PloS One,Aug 18；11(8)).
Summary of the invention
The object of the present invention is to provide a dystrophy Bacillus new species and its applications.
In a first aspect, the claimed one plant bacterial strain for belonging to dystrophy Bacillus novel bacterial.
The present invention bacterial strain claimed for belonging to dystrophy Bacillus novel bacterial is specially Modestobacter
deseti CPCC D00004.Deposit number of the bacterial strain in China Committee for Culture Collection of Microorganisms's common micro-organisms center
For CGMCC No.18004.
Second aspect, a kind of claimed microbial inoculum.
The active constituent of present invention microbial inoculum claimed is the Modestobacter deseti CPCC
D00004。
In above-mentioned microbial inoculum, the microbial inoculum can also include carrier.The carrier can be solid carrier or liquid-carrier.It is described
Solid carrier can be mineral material, vegetable material or high-molecular compound；The mineral material can for clay, talcum, kaolin,
At least one of montmorillonite, white carbon, zeolite, silica and diatomite；The vegetable material can be in corn flour, bean powder and starch
At least one；The high-molecular compound can be polyvinyl alcohol and/or polyglycols.The liquid-carrier can for organic solvent,
Vegetable oil, mineral oil or water；The organic solvent can be decane and/or dodecane.In the microbial inoculum, the active constituent can be with
It is deposited in the form of the fermentation liquid of the living cells, living cells that are cultured, the filtrate of cell culture or cell and the mixture of filtrate
In.The microbial inoculum dosage form can be a variety of dosage forms, such as liquor, emulsion, suspending agent, pulvis, granule, wettable powder or water dispersion
Granula.
As needed, surfactant (such as polysorbas20, Tween 80), adhesive, stabilization can be also added in the microbial inoculum
Agent (such as antioxidant), pH adjusting agent.
The third aspect, the claimed Modestobacter deseti CPCC D00004 or the microbial inoculum
It is following it is any in application:
(A1) degrading organic phosphor；
(A2) preparation is used for the product of degrading organic phosphor.
Fourth aspect, the claimed Modestobacter deseti CPCC D00004 or the microbial inoculum
It is following it is any in application:
(B1) alkaline phosphatase is prepared；
(B2) preparation has the product of alkaline phosphatase activities.
5th aspect, the claimed Modestobacter deseti CPCC D00004 or the microbial inoculum
It is following it is any in application:
(C1) acid phosphatase is prepared；
(C2) preparation has the product of activity of acid phosphatase.
6th aspect, the claimed Modestobacter deseti CPCC D00004 or the microbial inoculum
It is following it is any in application:
(D1) garbage disposal；
(D2) soil remediation；
(D3) control of desert；
(D4) soil improvement.
7th aspect, claimed following any substance:
(E1) degradation prepared using the Modestobacter deseti CPCC D00004 or the microbial inoculum is organic
The product of phosphorus；
(E2) alkaline phosphatase prepared using the Modestobacter deseti CPCC D00004 or the microbial inoculum
Enzyme or product with alkaline phosphatase activities；
(E3) acid phosphatase prepared using the Modestobacter deseti CPCC D00004 or the microbial inoculum
Enzyme or product with activity of acid phosphatase.
Further, in (E1), the active constituent of the product can be the Modestobacter deseti CPCC
D00004, or can be the alkaline phosphatase prepared using the Modestobacter deseti CPCC D00004 or the microbial inoculum
Enzyme and/or acid phosphatase.
Further, in (E2), the active constituent of the product can be the desert dystrophy bacillus
(Modestobacter deseti) CPCC D00004, or can be to utilize the desert dystrophy bacillus (Modestobacter
Deseti) CPCC D00004 or the alkaline phosphatase of microbial inoculum preparation.
Further, in (E3), the active constituent of the product can be the Modestobacter deseti CPCC
D00004, or can be the acid phosphatase prepared using the Modestobacter deseti CPCC D00004 or the microbial inoculum
Enzyme.
Eighth aspect, the claimed Modestobacter deseti CPCC D00004 is described in the preparation
Application in microbial inoculum.
In a specific embodiment of the invention, the organic phosphorus specially lecithin.It degrades the organic phosphorus temperature
Specially 28 DEG C.Organic phosphorus time of degrading is specially 14 days.The organic phosphorus environment pH that degrades is 7.2.Degradation institute
Carbon source when stating organic phosphorus is glucose.
9th aspect, claimed following bacterial strain or its application；
The bacterial strain is bacterial strain in Modestobacter deseti strain；The Modestobacter deseti strain
The 16S rRNA gene order of middle bacterial strain at least has 98.7% or more similitude compared with SEQ ID No.1；It is described
Bacterial strain is Gram positive aerobic heterotroph in Modestobacter deseti strain, and no sporogenesis does not move, corynebacterium
Cell tends to aggregate into group, and cell size is (1.0-2.8) μ m (1.0-3.0) μm, and bacterium colony is in thin and irregular orange；Bacterium
The growth tolerance range of strain is 15-37 DEG C, 0-3%NaCl, pH 6-8.0, and optimum growing condition is 28 DEG C, 0%NaCl, pH
7.0；Oxidizing ferment, catalase, trypsase, esterase (C4), lipoid esterase (C8), lipoidase (C14), cystine arylamine enzyme, white ammonia
Sour arylamine enzyme, valine arylamine enzyme, beta galactosidase, alpha-glucosidase, beta-glucosidase, alkaline phosphatase, acidity
The generation of phosphatase and naphthols-AS-BI- phosphohydrolase is positive；Gelatin liquefaction, indoles generation, Starch Hydrolysis, cellulose water
Solution and H2S is produced as feminine gender；Bacterial strain and Modestobacter belong to other kinds in the Modestobacter deseti strain
Bacterial strain compares peculiar C16:1ω 7c and C17:1Both fatty acid compositions of ω 8c；
The application be the bacterial strain it is following it is any in application:
(A1) degrading organic phosphor；
(A2) preparation is used for the product of degrading organic phosphor；
(B1) alkaline phosphatase is prepared；
(B2) preparation has the product of alkaline phosphatase activities；
(C1) acid phosphatase is prepared；
(C2) preparation has the product of activity of acid phosphatase；
(D1) garbage disposal；
(D2) soil remediation；
(D3) control of desert；
(D4) soil improvement.
It is demonstrated experimentally that bacterial strain CPCC D00004 of the present invention represents a new species of dystrophy Bacillus, it is named as
Modestobacter deseti.Demonstrating bacterial strain of the present invention simultaneously has alkaline phosphatase activities and activity of acid phosphatase,
It can be one plant of functional dystrophy bacillus new species, in garbage disposal, soil remediation, control of desert, soil property with degrading organic phosphor
Improvement etc. will be with a wide range of applications.
Preservation explanation
It is recommended that classification naming: Modestobacter deseti
Join the biomaterial (strain) of Ju: CPCC D00004
Preservation mechanism: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation mechanism abbreviation: CGMCC
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Preservation date: on June 19th, 2019
Collection is registered on the books number: CGMCC No.18004
Detailed description of the invention
Fig. 1 is CPCC D00004 bacterial strain in 28 DEG C of-GYM culture medium cultures, 5 days colonial morphology photos.
Fig. 2 is the N-J systematic evolution tree of the 16S rRNA gene order building based on the various representative strains of dystrophy Bacillus.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
The separation and identification of embodiment 1, bacterial strain CPCC D00004
One, the separation of bacterial strain CPCC D00004
Bacterial strain CPCC D00004 of the present invention is isolated from Ningxia Tengger desert soil, and specific lock out operation is as follows:
Soil supension preparation: by 2g pedotheque be added to 18mL it is sterile, containing 0.1% sodium pyrophosphate and 0.85% chlorine
In the mixed solution for changing sodium, it is placed in 28 DEG C of shaking tables 180 revs/min, concussion 40min, soil particle is made sufficiently to suspend.By formation
Soil supension carries out gradient dilution to 10﹣ 4It is spare.
Strain isolation culture medium: using M1 isolation medium, and nutritional ingredient is as follows: glucose 10gL-1, yeast extract
1g·L-1, beef extract 1gL-1, casein hydrolysate 2gL-1, agar 15gL-1, ddH2O 1L；pH 7.
Separation method: the good soil supension of above-mentioned dilution is coated on M1 isolation medium plate, and 28 DEG C are cultivated 3 weeks.
Picking well-grown, the single colonie that lawn is complete and morphological feature is different, in PYG (peptone 3gL-1, yeast extract 5gL-1,
Glycerol 10gL-1, glycine betaine 1.25gL-1, Sodium Pyruvate 1.25gL-1, agar 15gL-1；PH7 it) is trained on slant medium
It supports, pure culture is obtained, so as to follow-up study.Pure bacterial strain obtained is carried out using 20% (v/v) glycerol as protective agent simultaneously
Liquid nitrogen preservation and -80 DEG C of freezings.It obtains one plant and compares bacterium Modestobacter lapidis MON 3.1 with nearest edgeT
Similitude is 97.8% strain, and number is CPCC D00004.
Two, the identification of bacterial strain CPCC D00004
Bacterial strain CPCC D00004 is grown on GYM culture medium (fructus hordei germinatus leaching powder 10gL under the conditions of 28 DEG C-1, yeast extract
4g·L-1, glucose 4gL-1, calcium carbonate 2gL-1, agar 15gL-1；PH 7) on, carry out morphology, Physiology and biochemistry, thin
Born of the same parents' chemistry and gene level research, other special circumstances are by explanation.
1, the morphology of CPCC D00004 bacterial strain and physiological and biochemical property detection
Bacterial strain CPCC D00004 after cultivating 5 days on GYM solid medium, uses full-automatic bacterial colony counting instrument at 28 DEG C
(fast number) and Stereo microscope (OLYMPUS SZX7) carry out colony morphological observation.Growth temperature detection range be 4,10,15,
20,25,28,30,37,42 and 45 DEG C；Grow salinity (NaCl) detection range be 0-10% and 15% (0-10g/100mL and
12 (0,1,2,3,4,5,6,7,8,9,10 and 15) concentration gradients 15g/100mL)；PH detection range is grown between 4-10
7 (4,5,6,7,8,9,10) gradients (Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, et
al.Naxibacter alkalitolerans gen.nov.,sp.nov.,a novel member of the family
Oxalobacteraceae isolated from China.Int J Syst Evol Microbiol 2005；55:1149-
1153).Detection kit API 50CH, the API ZYM that the biochemical functions of bacterial strain are produced using Mei Liai company, France, with
And the detection system GEN III of BiOLOG company, U.S. production is completed.Other bacterial strain physiological characteristics, including Gram's staining category
Property, motility, oxygen demand, catalase activity, Starch Hydrolysis, gelatin liquefaction, indoles generate, H2S generate and cellulolytic activity
Deng, carried out referring especially to " actinomyces system identification handbook " (Xu L H.Actinomycete systematics:
principles,methods and practices[M].Beijing:Science Press,2007,93-108.)。
Qualification result shows, bacterial strain CPCC D00004 is the aerobic heterotroph of Gram-positive, no sporogenesis,
Do not move, corynebacterium cell tends to aggregate into group, and cell size is (1.0-2.8) μ m (1.0-3.0) μm, bacterium colony in it is thin without
The orange of rule, is shown in Fig. 1.Bacterial strain CPCC D00004 can be grown on oligotrophic culture medium, the GYM solid culture at 30 DEG C
After cultivating 5 days on base, the growth tolerance range of bacterial strain is 15-37 DEG C, 0-3%NaCl, pH 6-8.0, and optimum growing condition is 28
DEG C, 0%NaCl, pH 7.0.Oxidizing ferment, catalase, trypsase, esterase (C4), lipoid esterase (C8), lipoidase (C14), Guang ammonia
Sour arylamine enzyme, leucine arylamine enzyme, valine arylamine enzyme, beta galactosidase, alpha-glucosidase, beta-glucosidase, alkali
The generation of acid phosphatase, acid phosphatase and naphthols-AS-BI- phosphohydrolase is positive；Gelatin liquefaction, indoles generation, starch
Hydrolysis, cellulose hydrolysis and H2S is produced as feminine gender.Bacterial strain CPCC D00004 source species representative strain close with dystrophy Bacillus
Physiological and biochemical property difference is shown in Table 1.
1 bacterial strain CPCC D00004 of table source strain representative strain physiological and biochemical property difference table close with dystrophy Bacillus
Note: in table ,+it is expressed as the positive, W indicates weakly positive ,-it is expressed as feminine gender.
Result is as it can be seen that bacterial strain CPCC D00004 of the present invention and the nearly source bacterial strain registered are special in Physiology and biochemistry as shown in Table 1
Sign aspect has differences.
3, the cytochemistry feature detection of bacterial strain CPCC D00004
Cellular fat sour component (the Miller LT.Single of bacterial strain CPCC D00004 is detected by gas chromatographic analysis
derivatization method for routine analysis of bacterial whole-cell,fatty
acids methyl esters,including hydroxyl acids.J Clin Microbiol 1982；16:584-
586.).In bacterial strain CPCC D00004 of the present invention, MK-9 (H4) it is main breathing quinones type in respiratory chain, phosphatidyl ethanol
Amine (PE), phosphatidyl glycerol (PG), cardiolipin (DPG), phosphatidylinositols (PI) and phosphatidylinositol mannosidase
It (PIMs) is main polar lipid ingredient.Compare invention bacterial strain CPCC D00004 and nearly source strain representative strain M.lapidis
MON 3.1T、M.multiseptatus AA-826T、M.versicolor CP153-2T、M.marinus 42H12-1TRouge
Fat sour component, is shown in Table 2.The results show that the main fatty acid of bacterial strain CPCC D00004 of the present invention is iso-C16:0, account for total content
15.5%, this main component is also consistent with other kinds of dystrophy Bacillus.But bacterial strain CPCC D00004 of the present invention has again and it
His different distinctive feature of nearly source kind, such as C16:1ω 7c and C17:1ω 8c etc. is exactly its exclusive ingredient, while as shown in table 2, bacterium
The content of the strain many fatty acid compositions of CPCC D00004 is also different from other nearly source kinds, therefore bacterial strain CPCC D00004 of the present invention
It is dystrophy Bacillus new species.
2 bacterial strain CPCC D00004 of table source strain representative strain fatty acid characteristic difference table close with dystrophy Bacillus
Note: "-" expression does not detect in table
4, the alkaline phosphatase activities detection of bacterial strain CPCC D00004
Bacterial strain CPCC D00004 of the present invention is measured the degrading activity of organic phosphorus compound using liquid screening method
(Schrenkhammer P,Rosnizeck I C,Duerkop A,et al.Time-resolved fluorescence-
based assay for the determination of alkaline phosphatase activity and
application to the screening of its inhibitors[J].Journal of Biomolecular
Screening,2007,13(1):9-16.).Organic phosphorus fluid nutrient medium is prepared, solvent is water, and solute and concentration are as follows: lecithin
Rouge 0.5gL-1, glucose 10gL-1, Magnesium dichloride hexahydrate 5gL-1, bitter salt 0.25gL-1, potassium chloride
0.2g·L-1, ammonium sulfate 0.1gL-1, pH 7.2.Bacterial strain CPCC D00004 in logarithmic growth phase is connect with 5% (v/v)
Kind amount is inoculated into the organic phosphorus fluid nutrient medium of 20mL, and not connect the blank cultures of bacterium as a control group, is trained in 28 DEG C
After supporting 14 days, centrifuging and taking supernatant measures organic phosphorus concentration in culture solution according to the method recorded in above-mentioned document, and with blank
Group calculates the PO that bacterial strain CPCC D00004 degrading organic phosphor generates as control4 3-(mg/L) concentration variation (uses △ PO4 3-Table
Show).
△PO4 3-Concentration=M1-M0
Wherein, M0 is the PO detected in blank control group4 3-Concentration, M1 are the PO detected in experimental group4 3-Concentration.
It is computed the △ PO that bacterial strain CPCC D00004 of the present invention is generated with alkaline phosphatase activities, degrading organic phosphor4 3-
Concentration value is 10.07mg/L.Bacterial strain CPCC D00004 further is detected in the genome level of CPCC D00004 simultaneously
Containing alkaline phosphatase A gene (pho A) and alkaline phosphatase D (pho D) gene, it is shown in Table 3.To sum up, from phenotype and genotype
In level, prove that bacterial strain CPCC D00004 of the present invention has alkaline phosphatase activities.
The comparison result of alkaline phosphatase gene in the database in 3 bacterial strain CPCC D00004 genome of table
5, the determination of bacterial strain phyletic evolution status
The genomic DNA for extracting bacterial strain CPCC D00004 of the present invention is sequenced, and by 16S rRNA gene sequence therein
Arrange (as shown in SEQ ID No.1) internal authority systematic bacteriology analytical database (http: //
Www.ezbiocloud.net/ (Kim OS, Cho YJ, Lee K, et al.2012, Introducing are compared online in)
EzTaxon-e:a prokaryotic 16S rRNA gene sequence database with phylotypes that
represent uncultured species.Int J Syst Evol Microbiol,62:716-721.).As the result is shown
The strain similitude highest of bacterial strain CPCC D00004 of the present invention and dystrophy Bacillus, wherein the sequence highest object of similitude two-by-two
Kind representative strain is respectively M.lapidis MON 3.1T(similitude 97.8%) and M.multiseptatus AA-826TIt is (similar
97.2%) property is.It can be seen that the highest of the 16S rRNA gene order of bacterial strain CPCC D00004 of the present invention and known strain
Similitude is 97.8%, which defines (the bibliography: Stackebrandt of threshold value 98.7% significantly lower than bacterium different plant species
E,Ebers J.2006,Taxonomic parameters revisited:tarnished gold
standards.Microbiol Today,33:152-155.).For the phyletic evolution status for further clarifying bacterial strain, choose poor
The 16S rRNA gene order for supporting the representative strain of all strains of Bacillus constructs systematic evolution tree (Fig. 2).To further clarify
The phyletic evolution status of bacterial strain relatively and calculates the whole genome sequence of bacterial strain CPCC D00004 and close on EZbiocloud
Edge compares the average nucleotide similarity (ANI value) of the whole genome sequence of bacterium, calculates bacterial strain CPCC D00004 and compares with nearly edge
Bacterium M.versicolor CP153-2TWith M.marinus 42H12-1TSimilitude 73.6% and 74.1%, lower than divide two
ANI value (95%) (Yoon SH, Ha SM, Lim J, Kwon S, the Chun J.A large-scale of gene kind
evaluation of algorithms to calculate average nucleotide identity.Antonie van
Leeuwenhoek 2017；110:1281-1286.).Based on this, bacterial strain CPCC D00004 of the present invention is determined as dystrophy Bacillus
A new species.
In conclusion bacterial strain CPCC D00004 of the present invention and existing dystrophy Bacillus genus strain phenotype, Physiology and biochemistry and
There are many significant difference characteristics for cytochemistry feature etc..The phylogenetic analysis based on gene level is further simultaneously
It illustrates the difference of the bacterial strain Yu existing each species of dystrophy bacillus, sufficiently demonstrates bacterial strain CPCC D00004 of the present invention and represent
One new species of dystrophy Bacillus, are named as Modestobacter deseti.Bacterial strain CPCC D00004 of the present invention has alkali
Acid phosphatase activity and activity of acid phosphatase, can be one plant of functional dystrophy bacillus new species, in rubbish with degrading organic phosphor
Rubbish processing, soil remediation, control of desert, soil improvement etc. will be with a wide range of applications.
Modestobacter deseti CPCC D00004 has been preserved on 06 19th, 2019 Chinese common micro-
Biological inoculum preservation administrative center, deposit number are CGMCC No.18004.
<110>Inst. of Medicinal Biological Technology, Chinese Academy of Medical Sciences
<120>dystrophy Bacillus new species and its application
<130> GNCLN191598
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1455
<212> DNA
<213> Modestobacter deseti
<400> 1
actggcgggt gcttaccatg caagtcgagc gaggcccgct cttcggggtg gtgccctagc 60
ggcgaacggg tgagtaacac gtgggcaacc tgccctcagc tctgggataa ccccaagaaa 120
ttggggctaa tactggatat gactgctgac cgcatggtct ggtggtggaa agatttatcg 180
gctgaggatg ggcccgcggc ctatcagctt gttggtgggg tgatggccta ccaaggcgac 240
gacgggtagc cggcctgaga gggtgaccgg tcacactggg actgagacac ggcccagact 300
cctacgggag gcagcagtgg ggaatattgc gcaatgggcg gaagcctgac gcagcgacgc 360
cgcgtggggg atgacggcct tcgggttgta aacctctttc agcagggacg aagcgaaagt 420
gacggtacct gcagaagaag caccggccaa ctacgtgcca gcagccgcgg taatacgtag 480
ggtgcaagcg ttgtccggaa ttattgggcg taaagagctc gtaggcggtc tgtcgcgtcg 540
gctgtgaaat cccgaggctc aacctcgggt ctgcagtcga tacgggcaaa ctagagtgtt 600
gcaggggaga ctggaattcc tggtgtagcg gtgaaatgcg cagatatcag gaggaacacc 660
ggtggcgaag gcgggtctct gggcaacaac tgacgctgag gagcgaaagc gtggggagcg 720
aacaggatta gataccctgg tagtccacgc cgtaaacgtt gggcgctagg tgtgggggcc 780
attccacggt ctccgtgccg cagctaacgc attaagcgcc ccgcctgggg agtacggccg 840
caaggctaaa actcaaagga attgacgggg gcccgcacaa gcggcggagc atgttgctta 900
attcgatgca acgcgaagaa ccttacctag gcttgacatg tgcggaaatc ctccagagat 960
ggtgggtccg taagggtcgc acacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag 1020
atgttgggtt aagtcccgca acgagcgcaa ccctcgttcc atgttgccag cacgttatgg 1080
tggggactca tgggagactg ccggggtcaa ctcggaggaa ggtggggatg acgtcaaatc 1140
atcatgcccc ttatgtctag ggctgcaaac atgctacaat ggccggtaca aagggctgcg 1200
ataccgtgag gtggagcgaa tcccaaaaag ccggtctcag ttcggattgg ggtctgcaac 1260
tcgaccccat gaagttggag tcgctagtaa tcgcagatca gcaacgctgc ggtgaatacg 1320
ttcccgggcc ttgtacacac cgcccgtcac gtcacgaaag tcggtaacgc ccgaagccgg 1380
gggcccaacc cttgtggagg gagccgtcga aggcgggatc ggcgattggg acgaagtcgt 1440
aacaagatgc ccccc 1455
Claims (10)
1. bacterial strain, it is characterised in that: the bacterial strain is Modestobacter deseti CPCC D00004, in the micro- life of China
The deposit number of object culture presevation administration committee common micro-organisms center is CGMCC No.18004.
2. a kind of microbial inoculum, active constituent is bacterial strain described in claim 1.
3. bacterial strain described in claim 1 or microbial inoculum as claimed in claim 2 it is following it is any in application:
(A1) degrading organic phosphor；
(A2) preparation is used for the product of degrading organic phosphor.
4. bacterial strain described in claim 1 or microbial inoculum as claimed in claim 2 it is following it is any in application:
(B1) alkaline phosphatase is prepared；
(B2) preparation has the product of alkaline phosphatase activities；
(C1) acid phosphatase is prepared；
(C2) preparation has the product of activity of acid phosphatase.
5. bacterial strain described in claim 1 or microbial inoculum as claimed in claim 2 it is following it is any in application:
(D1) garbage disposal；
(D2) soil remediation；
(D3) control of desert；
(D4) soil improvement.
6. following any substance:
(E1) product of the degrading organic phosphor prepared using bacterial strain described in claim 1 or microbial inoculum as claimed in claim 2；
(E2) it utilizes the alkaline phosphatase of bacterial strain described in claim 1 or microbial inoculum as claimed in claim 2 preparation or there is alkali
The active product of acid phosphatase；
(E3) it utilizes the acid phosphatase of bacterial strain described in claim 1 or microbial inoculum as claimed in claim 2 preparation or there is acid
The active product of acid phosphatase.
7. substance according to claim 6, it is characterised in that: in (E1), the active constituent of the product is the bacterium
Strain, or alkaline phosphatase and/or acid phosphatase to be prepared using the bacterial strain or the microbial inoculum；Or
In (E2), the active constituent of the product is the bacterial strain, or the alkali to be prepared using the bacterial strain or the microbial inoculum
Acid phosphatase；Or
In (E3), the active constituent of the product is the bacterial strain, or the acid to be prepared using the bacterial strain or the microbial inoculum
Acid phosphatase.
8. application of the bacterial strain described in claim 1 in the microbial inoculum described in preparation claim 2.
9. substance described in application according to claim 3 or claim 7 or 8, it is characterised in that: described organic phosphorus to be
Lecithin.
10. bacterial strain or its application；
The bacterial strain is bacterial strain in Modestobacter deseti strain；Bacterium in the Modestobacter deseti strain
The 16S rRNA gene order of strain at least has 98.7% or more similitude compared with SEQ ID No.1；It is described
Bacterial strain is Gram positive aerobic heterotroph in Modestobacter deseti strain；The Modestobacter deseti
Bacterial strain peculiar C compared with Modestobacter belongs to the bacterial strain of other kinds in strain16:1ω 7c and C17:1Both fatty acid of ω 8c
Ingredient；
The application be the bacterial strain it is following it is any in application:
(A1) degrading organic phosphor；
(A2) preparation is used for the product of degrading organic phosphor；
(B1) alkaline phosphatase is prepared；
(B2) preparation has the product of alkaline phosphatase activities；
(C1) acid phosphatase is prepared；
(C2) preparation has the product of activity of acid phosphatase；
(D1) garbage disposal；
(D2) soil remediation；
(D3) control of desert；
(D4) soil improvement.
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Cited By (1)
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| CN114774330A (en) * | 2022-05-27 | 2022-07-22 | 中国医学科学院医药生物技术研究所 | Novel species of cellulolytic female phytophthora and application thereof |
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