CN110484461B - Novel species of genus anaerophytic bacillus and use thereof - Google Patents
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Abstract
The invention discloses a new species of the genus anaerophytic bacillus and application thereof. The invention provides a Modstopbacter desetti CPCC D00004 with the preservation number of CGMCC No.18004 in China general microbiological culture Collection center. The strain CPCC D00004 has alkaline phosphatase activity and acid phosphatase activity, can degrade organic phosphorus, is a new species of functional poor-culture bacillus, and has wide application prospect in the aspects of garbage treatment, soil remediation, desert control, soil improvement and the like.
Description
Technical Field
The invention relates to the field of microorganisms, in particular to a novel species of the genus anaerophytic bacillus and application thereof.
Background
The genus anaerophytic (modelobacter) belongs to the bacterial domain (Bacteria) -Actinomycetes (Actinomobacteria) -Frankles (Frankiales) -Dermatophagaceae (Geodermophilaceae).
In 2000, 4 strains of psychrophilic actinomycetes, numbered AA-802, AA-824, AA-825 and AA-826, were discovered by Mevs U et al. The 16S rRNA gene sequence information of these strains showed that they were highly similar to the strains of Geodermatophilus (Geodermatophilus) and Blastocococcus (Blastococcus), and that the strains AA-802, AA-824, AA-825 and AA-826 formed separate branches on phylogeny constructed by 16S rRNA gene sequence analysis and were adjacent to the strains of Geodermatophilus and Blastococcus. By integrating physiological and biochemical characteristics and cytochemical classification indexes, the model strain is a model strain of multi-septum poor-culture bacillus (Modostobacter), and the strain AA-826 is used as the model strain. In 2006, Normand P formally proposed a classification unit of Geodermatophilaceae (Geodermaphilaceae) and described Geodermatophilaceae, which were assigned to the genus Geodermatophilaceae, Nov, a for the description. int Syst Evol Microbiol.2006Oct, 56(Pt 10): 2277-8).
At present, the poor-nutrition bacillus comprises 9 species, most of which are from arid desert soil, and also has life-critical environments separated from biological soil crust, salt-tolerant plant rhizosphere, deep sea bottom mud, rock surface and the like. It has been reported that strains of the genus anaerophyte have properties of radiation resistance, drought resistance and protection of soil against erosion and are therefore among the pioneer microorganisms in desert environments (Martha E, Trujillo, Michael Goodfellow, Kanungnid Busarakam, Raul Riesco.Modostobacter lapidis sp.nov.and Modstobacter miralis sp.nov., isolated from a predetermined saline soil building in Salamanca, Spain. Antonie van Leeuwenhoek,2015,108: 311-320).
In recent years, the organic phosphorus in pesticides and fertilizers is enriched in the environment, which causes the global water quality degradation problem and frequent outbreak of toxic water bloom. The microbial remediation technology for treating pollutants by using environmental organisms with microbial degradation as a core has the remarkable advantages of high efficiency, environmental protection, economy and the like, and is widely concerned by scholars. Although many strains of lysogenum have been isolated in various habitats, few reports have been made on strains of the genus anaerophyta; there is only One report of the phosphorus solubilizing activity of strains of the genus anaerophyticus (Zhang BH, Salam N, Cheng J, et al 2016. Modostobacter lactis Microchip sp. nov., phosphor-soluble bacterium with ability to promote microorganisms growth microbiology. PloS One, Aug 18; 11 (8)).
Disclosure of Invention
The invention aims to provide a new species of the genus anaerophytic bacillus and application thereof.
In a first aspect, the invention claims a strain belonging to a new species of the genus anaerophytic bacteria.
The invention claims a new strain belonging to the genus anaerophytic bacillus, in particular a modelobacter desetti CPCC D00004. The preservation number of the strain in China general microbiological culture Collection center is CGMCC No. 18004.
In a second aspect, the invention claims a bacterial agent.
The active ingredient of the microbial inoculum required by the invention is the modelockacter desetti CPCC D00004.
In the above microbial inoculum, the microbial inoculum may further comprise a carrier. The carrier may be a solid carrier or a liquid carrier. The solid carrier can be a mineral material, a plant material or a high molecular compound; the mineral material may be at least one of clay, talc, kaolin, montmorillonite, white carbon, zeolite, silica, and diatomaceous earth; the plant material may be at least one of corn flour, bean flour and starch; the high molecular compound may be polyvinyl alcohol and/or polyglycol. The liquid carrier can be an organic solvent, vegetable oil, mineral oil, or water; the organic solvent may be decane and/or dodecane. In the microbial inoculum, the active ingredient may be present in the form of cultured living cells, a fermentation broth of living cells, a filtrate of a cell culture, or a mixture of cells and a filtrate. The preparation formulation of the microbial inoculum can be various formulations, such as liquid, emulsion, suspending agent, powder, granules, wettable powder or water dispersible granules.
According to the requirement, the microbial inoculum can also be added with a surfactant (such as Tween 20, Tween 80 and the like), a binder, a stabilizer (such as an antioxidant), a pH regulator and the like.
In a third aspect, the invention claims the application of the modelockacter deseti CPCC D00004 or the microbial inoculum in any one of the following:
(A1) degrading organic phosphorus;
(A2) preparing products for degrading organic phosphorus.
In a fourth aspect, the invention claims the application of the modelockacter deseti CPCC D00004 or the microbial inoculum in any one of the following:
(B1) preparing alkaline phosphatase;
(B2) preparing a product having alkaline phosphatase activity.
In a fifth aspect, the invention claims the use of said modelockacter desetti CPCC D00004 or said microbial inoculum in any of:
(C1) preparing acid phosphatase;
(C2) preparing a product having acid phosphatase activity.
In a sixth aspect, the invention claims the use of said modelockacter deseti CPCC D00004 or said microbial inoculum in any of:
(D1) treating garbage;
(D2) soil remediation;
(D3) treating desert;
(D4) the soil quality is improved.
In a seventh aspect, the invention claims any of the following:
(E1) an organophosphorus degradation product prepared by the Modstobacter desetti CPCC D00004 or the microbial inoculum;
(E2) alkaline phosphatase prepared by the modelockacter desetti CPCC D00004 or the microbial inoculum or a product with alkaline phosphatase activity;
(E3) acid phosphatase prepared by the modelobacter deseti CPCC D00004 or the microbial inoculum or a product with acid phosphatase activity.
Further, in (E1), the active ingredient of the product may be the modelockbacter deseti CPCC D00004, or may be alkaline phosphatase and/or acid phosphatase prepared using the modelockbacter deseti CPCC D00004 or the microbial agent.
Further, in (E2), the active ingredient of the product may be the desert poor-cultured bacillus (modelockacter desenti) CPCC D00004, or may be alkaline phosphatase prepared using the desert poor-cultured bacillus (modelockacter desenti) CPCC D00004 or the microbial agent.
Further, in (E3), the active ingredient of the product may be the modelockacter deseti CPCC D00004, or may be acid phosphatase prepared using the modelockacter deseti CPCC D00004 or the microbial agent.
In an eighth aspect, the invention claims the application of the modelockacter deseti CPCC D00004 in preparing the microbial inoculum.
In a particular embodiment of the invention, the organophosphorus is in particular lecithin. The temperature for degrading the organic phosphorus is specifically 28 ℃. The time for degrading the organophosphorus was specifically 14 days. The pH of the environment in which the organophosphorus was degraded was 7.2. And the carbon source for degrading the organic phosphorus is glucose.
In a ninth aspect, the invention claims the following strains or uses thereof;
the strain is a strain in a Modstobacter desetti strain; compared with the SEQ ID No.1, the 16S rRNA gene sequence of the strain in the Modstobacter desetti strain has at least more than 98.7 percent of similarity; the strain in the Modstobacter deselti strain is gram-positive aerobic heterotrophic bacteria, does not have spore formation and does not move, short rod-shaped cells tend to aggregate into clusters, the cell size is (1.0-2.8) Mumx (1.0-3.0) Mum, and bacterial colonies are thin and irregular orange; the growth tolerance range of the strain is 15-37 ℃, 0-3% NaCl and pH 6-8.0, and the optimal growth condition is 28 ℃, 0% NaCl and pH 7.0; positive for production of oxidase, catalase, trypsin, esterase (C4), lipoid esterase (C8), lipase-like enzyme (C14), cystine arylamine, leucine arylamine, valine arylamine, β -galactosidase, α -glucosidase, β -glucosidase, alkaline phosphatase, acid phosphatase, and naphthol-AS-BI-phosphohydrolase; gelatin liquefaction, indole production, starch hydrolysis, cellulose hydrolysis and H2S is negative; the strain in the strain of Modostobacter desenti has characteristic C compared with the strain of other strains of Modostobacter16:1ω 7C and C17:1Two fatty acid components, omega 8 c;
the application is the application of the strain in any one of the following steps:
(A1) degrading organic phosphorus;
(A2) preparing a product for degrading organic phosphorus;
(B1) preparing alkaline phosphatase;
(B2) preparing a product having alkaline phosphatase activity;
(C1) preparing acid phosphatase;
(C2) preparing a product having acid phosphatase activity;
(D1) treating garbage;
(D2) soil remediation;
(D3) treating desert;
(D4) the soil quality is improved.
Experiments prove that the strain CPCC D00004 represents a new species of the genus anaerophyte and is named as modelockacter deseti. Meanwhile, the bacterial strain has alkaline phosphatase activity and acidic phosphatase activity, can degrade organic phosphorus, is a new species of functional poor-culture bacillus, and has wide application prospects in the aspects of garbage treatment, soil remediation, desert control, soil improvement and the like.
Deposit description
Suggested classification nomenclature: modstobacter desetti
According to the biological materials (strains): CPCC D00004
The preservation organization: china general microbiological culture Collection center
The preservation organization is abbreviated as: CGMCC (China general microbiological culture Collection center)
Address: xilu No.1 Hospital No. 3 of Beijing market facing Yang district
The preservation date is as follows: 6 and 19 months in 2019
Registration number of the preservation center: CGMCC No.18004
Drawings
FIG. 1 is a photograph showing the colony morphology of the strain CPCC D00004 cultured in the medium 28 ℃ to GYM for 5 days.
FIG. 2 is a N-J phylogenetic tree constructed based on the 16S rRNA gene sequences of various representative strains of the genus anaerophytium.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1 isolation and identification of Strain CPCC D00004
Separation of strain CPCC D00004
The strain CPCC D00004 is separated from Ningxia Teng desert soil, and the specific separation operation is as follows:
preparing a soil suspension: 2g of soil sample was added to 18mL of a sterile mixed solution containing 0.1% sodium pyrophosphate and 0.85% sodium chloride, and the mixture was placed in a shaker at 28 ℃ for 180 rpm and shaken for 40min to suspend the soil particles sufficiently. The resulting soil suspension was diluted to 10 degrees in a gradient﹣4And (5) standby.
Strain isolation medium: the culture medium was isolated using M1, and the nutrients were as follows: glucose 10 g.L-1Yeast extract 1 g.L-1Beef extract 1 g.L-1Casein hydrolysate 2 g.L-1Agar 15 g. L-1，ddH2O 1L；pH 7。
The separation method comprises the following steps: the diluted soil suspension was applied to M1 medium plates and incubated at 28 ℃ for 3 weeks. Selecting single colony with good growth, intact lawn and different morphological characteristics, and adding into PYG (peptone 3 g. L)-1Yeast extract 5 g.L-1Glycerol 10 g.L-1Betaine 1.25 g.L-1Sodium pyruvate 1.25 g.L-1Agar 15 g. L-1(ii) a pH7) on slant medium to obtain pure culture for subsequent study. Meanwhile, the obtained pure strain is preserved by liquid nitrogen and frozen at-80 ℃ by taking 20% (v/v) glycerol as a protective agent. Obtaining a strain of Modestobacter lapidis MON 3.1 which is a strain of the most recent control bacteriumTThe similarity was 97.8% strain, numbered CPCC D00004.
II, identification of strain CPCC D00004
The strain CPCC D00004 grows in GYM culture medium (malt extract powder 10 g.L) at 28 deg.C-1Yeast extract powder 4 g.L-1Glucose 4 g.L-1Calcium carbonate 2 g.L-1Agar 15 g. L-1(ii) a pH7), morphological, physiological biochemical, cytochemical and gene level studies, other special cases will be described.
1. Morphological and physiological and biochemical characteristic detection of CPCC D00004 strain
After the strain CPCC D00004 was cultured on GYM solid medium at 28 ℃ for 5 days, colony morphology was observed using a full-automatic colony counter (rapid count) and a body microscope (OLYMPUS SZX 7). The growth temperature detection range is 4, 10, 15, 20, 25, 28, 30, 37, 42 and 45 ℃; growth salt concentration (NaCl) was measured over 12 (0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 15) concentration gradients ranging from 0-10% and 15% (0-10g/100mL and 15g/100 mL); growth pH was measured in 7 (4, 5, 6, 7, 8, 9, 10) gradients (Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, et al, Naxibacterium alkallitanterans gen. nov., sp. nov., a novel member of the family Oxalobacterium isolated from China. int J Syst Evol Microbiol 2005; 55: 1149-. The physiological and biochemical functions of the strain are completed by using detection kits API 50CH and API ZYM produced by French Meriella, and a detection system GEN III produced by American BiOLOG. Other physiological characteristics of the strain, including gram stain profile, motility, oxygen demand, catalase activity, starch hydrolysis, gelatin liquefaction, indole production, H2S production, cellulose hydrolysis activity, and the like, are mainly described in "Actinomycetes systems identification Manual" (Xu L. Actinomycetes systems: principles, methods and practices [ M ]. Beijing: Science Press,2007, 93-108.).
The identification result showed that the strain CPCC D00004 is a gram-positive aerobic heterotrophic bacterium, has no sporulation and no movement, the short rod-shaped cells tend to aggregate into clusters, the cell size is (1.0-2.8) mum x (1.0-3.0) mum, and the colonies are in a thin and irregular orange color, as shown in FIG. 1. The strain CPCC D00004 can grow on an oligotrophic culture medium, after the strain is cultured on a GYM solid culture medium for 5 days at the temperature of 30 ℃, the growth tolerance range of the strain is 15-37 ℃, 0-3% NaCl and pH 6-8.0, and the optimal growth conditions are 28 ℃, 0% NaCl and pH 7.0. Positive for production of oxidase, catalase, trypsin, esterase (C4), lipoid esterase (C8), lipase-like enzyme (C14), cystine arylamine, leucine arylamine, valine arylamine, β -galactosidase, α -glucosidase, β -glucosidase, alkaline phosphatase, acid phosphatase, and naphthol-AS-BI-phosphohydrolase; liquefaction of gelatinIndole production, starch hydrolysis, cellulose hydrolysis and H2S production was negative. The differences in physiological and biochemical characteristics between the strain CPCC D00004 and the strain represented by the closely-derived species of the genus anaerophyte are shown in Table 1.
TABLE 1 physiological and biochemical characteristics difference table between strain CPCC D00004 and closely-derived strain of genus anaerophyte
Note: in the table, + indicates positive, W indicates weak positive, and-indicates negative.
As can be seen from the results shown in Table 1, the strain CPCC D00004 of the present invention has differences in physiological and biochemical characteristics from the reported closely-derived strain.
3. Detection of cytochemical characteristics of strain CPCC D00004
The cellular fatty acid content of strain CPCC D00004 was determined by gas chromatographic analysis (Miller LT. Single inactivation method for route analysis of bacterial white-cells, fatty acids methyl esters, encapsulating hydroxyl acids. J Clin Microbiol 1982; 16:584 586.). In the present strain CPCC D00004, MK-9 (H)4) Is the main respiratory quinone type in the respiratory chain, Phosphatidylethanolamine (PE), Phosphatidylglycerol (PG), Diphosphatidylglycerol (DPG), Phosphatidylinositol (PI), and phosphatidylinositol mannosides (PIMs) are the main polar lipid components. Comparing the inventive strain CPCC D00004 with the closely-sourced strain representative strain M.lapidis MON 3.1T、M.multiseptatus AA-826T、M.versicolor CP153-2T、M.marinus 42H12-1TSee table 2 for fatty acid components of (a). The results show that the main fatty acid of the strain of the invention CPCC D00004 is iso-C16:015.5% of the total content, this main component also corresponds to other species of the genus anaemia. However, the strain CPCC D00004 of the invention has a unique feature different from other closely related species, such as C16:1ω 7C and C17:1Omega 8c and the like are unique components, and as shown in Table 2, the content of a plurality of fatty acid components of the strain CPCC D00004 is different from that of other closely-sourced species, so that the strain CPCC D00004 is a new species of the anoxybacter.
TABLE 2 fatty acid characteristic difference table between strain CPCC D00004 and a strain represented by a near-source strain of the genus anaerophyte
Note: in the table, "-" indicates no detection
4. Alkaline phosphatase Activity detection of Strain CPCC D00004
The degrading activity of the strain CPCC D00004 of the present invention on organophosphorus compounds was determined by liquid screening (Schrenkhammer P, Rosnizick I C, Duerkop A, et al, time-delayed fluorescence-based assay for the determination of alkaline phosphorus activity and application to the screening of inorganic inhibitors J]Journal of Biomolecular Screening,2007,13(1): 9-16.). Preparing an organic phosphorus liquid culture medium, wherein the solvent is water, and the solutes and the concentrations are as follows: lecithin 0.5 g.L-1Glucose 10 g.L-1Magnesium chloride hexahydrate 5 g.L-1Magnesium sulfate heptahydrate 0.25 g.L-10.2 g.L of potassium chloride-1Ammonium sulfate 0.1 g.L-1pH 7.2. Inoculating 5% (v/v) inoculum of strain CPCC D00004 in logarithmic growth phase into 20mL of organic phosphorus liquid culture medium, taking blank culture medium without inoculation as a control group, culturing at 28 ℃ for 14 days, centrifuging, taking supernatant, measuring the concentration of organic phosphorus in the culture solution according to the method described in the above document, taking blank as a control group, and calculating PO generated by degrading organic phosphorus by strain CPCC D000044 3-(mg/L) concentration Change (with. DELTA.PO)4 3-Representation).
△PO4 3-Concentration of M1-M0
Wherein M0 is PO detected in blank control group4 3-Concentration, M1 is PO detected in the experimental group4 3-And (4) concentration.
The bacterial strain CPCC D00004 of the invention has alkaline phosphatase activity and is calculated to degrade the delta PO generated by organic phosphorus4 3-The concentration value was 10.07 mg/L. At the same time, further on the genomic level of CPCC D00004, strain CPCC D00004 was detected to contain the alkaline phosphatase a gene (pho a) and the alkaline phosphatase D (pho D) gene, see table 3. In summary, the strain CPCC D00004 of the present invention was demonstrated to have alkaline phosphatase activity both in phenotypic and genotypic levels.
TABLE 3 alignment of alkaline phosphatase genes in the genome of Strain CPCC D00004 in the database
5. Determination of phylogenetic position of strain
Genomic DNA of the strain CPCC D00004 of the invention is extracted for sequencing, and the 16S rRNA gene sequence (shown as SEQ ID No. 1) is aligned online in an international authoritative bacteria taxonomy analysis database (http:// www.ezbiocloud.net /) (Kim OS, Cho YJ, Lee K, et al 2012, innovative EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes being present in the unknown space. int J Syst Evol Microbiol,62: 716-. The result shows that the strain CPCC D00004 has the highest similarity with the strains of the genus anaerophyte, wherein the representative strains of the species with the highest sequence pairwise similarity are respectively M.lapidis MON 3.1T(similarity 97.8%) and M.multiseptatus AA-826T(similarity 97.2%). It can be seen that the highest similarity of the 16S rRNA gene sequence of the strain CPCC D00004 of the invention to known species is 97.8%, which is significantly below the defined threshold of 98.7% for different species of bacteria (ref: Stackelbrandt E, Ebers J.2006, Taxomic parameters obtained: tarnished gold standards. Microbiol Today,33: 152-. To further clarifyPhylogenetic position of the strains, 16S rRNA gene sequences of representative strains of all species of the genus anaerophytes were selected to construct phylogenetic trees (FIG. 2). To further clarify the phylogenetic status of the strains, the average nucleotide similarity (ANI value) of the whole genome sequence of the strain CPCC D00004 and the whole genome sequence of the closely related control strain was compared and calculated on EZbiocloud, and the strain CPCC D00004 and the closely related control strain M.versicolor CP153-2 were calculatedTMarinus 42H12-1TThe similarity of 73.6% and 74.1% is lower than the ANI value (95%) for dividing two gene species (Yoon SH, Ha SM, Lim J, Kwon S, Chun J.A large-scale evaluation of algorithm to algorithm average nucleotide identity. Antonie van Leeuwenhoek 2017; 110: 1281-1286.). Based on this, the strain of the invention CPCC D00004 was identified as a new species of the genus anaerophyte.
In conclusion, the strain CPCC D00004 of the invention has a plurality of significant difference characteristics with the existing strains of the genus anaerophyta in the aspects of phenotype, physiological and biochemical characteristics, cytochemical characteristics and the like. Meanwhile, the phylogenetic analysis based on the gene level further proves that the strain is different from various species of the existing poor-nutrition bacillus, and fully proves that the strain CPCC D00004 represents a new species of the poor-nutrition bacillus and is named as Modstobacter desetti. The strain CPCC D00004 has alkaline phosphatase activity and acid phosphatase activity, can degrade organic phosphorus, is a new species of functional poor-culture bacillus, and has wide application prospect in the aspects of garbage treatment, soil remediation, desert control, soil improvement and the like.
The Modstobacter desetti CPCC D00004 has been preserved in 19.06.2019 in China general microbiological culture collection center with the preservation number of CGMCC No. 18004.
<110> institute of medical and Biotechnology of Chinese academy of medical sciences
<120> novel species of genus anaerophytic bacteria and uses thereof
<130> GNCLN191598
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1455
<212> DNA
<213> Modestobacter deseti
<400> 1
actggcgggt gcttaccatg caagtcgagc gaggcccgct cttcggggtg gtgccctagc 60
ggcgaacggg tgagtaacac gtgggcaacc tgccctcagc tctgggataa ccccaagaaa 120
ttggggctaa tactggatat gactgctgac cgcatggtct ggtggtggaa agatttatcg 180
gctgaggatg ggcccgcggc ctatcagctt gttggtgggg tgatggccta ccaaggcgac 240
gacgggtagc cggcctgaga gggtgaccgg tcacactggg actgagacac ggcccagact 300
cctacgggag gcagcagtgg ggaatattgc gcaatgggcg gaagcctgac gcagcgacgc 360
cgcgtggggg atgacggcct tcgggttgta aacctctttc agcagggacg aagcgaaagt 420
gacggtacct gcagaagaag caccggccaa ctacgtgcca gcagccgcgg taatacgtag 480
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gctgtgaaat cccgaggctc aacctcgggt ctgcagtcga tacgggcaaa ctagagtgtt 600
gcaggggaga ctggaattcc tggtgtagcg gtgaaatgcg cagatatcag gaggaacacc 660
ggtggcgaag gcgggtctct gggcaacaac tgacgctgag gagcgaaagc gtggggagcg 720
aacaggatta gataccctgg tagtccacgc cgtaaacgtt gggcgctagg tgtgggggcc 780
attccacggt ctccgtgccg cagctaacgc attaagcgcc ccgcctgggg agtacggccg 840
caaggctaaa actcaaagga attgacgggg gcccgcacaa gcggcggagc atgttgctta 900
attcgatgca acgcgaagaa ccttacctag gcttgacatg tgcggaaatc ctccagagat 960
ggtgggtccg taagggtcgc acacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag 1020
atgttgggtt aagtcccgca acgagcgcaa ccctcgttcc atgttgccag cacgttatgg 1080
tggggactca tgggagactg ccggggtcaa ctcggaggaa ggtggggatg acgtcaaatc 1140
atcatgcccc ttatgtctag ggctgcaaac atgctacaat ggccggtaca aagggctgcg 1200
ataccgtgag gtggagcgaa tcccaaaaag ccggtctcag ttcggattgg ggtctgcaac 1260
tcgaccccat gaagttggag tcgctagtaa tcgcagatca gcaacgctgc ggtgaatacg 1320
ttcccgggcc ttgtacacac cgcccgtcac gtcacgaaag tcggtaacgc ccgaagccgg 1380
gggcccaacc cttgtggagg gagccgtcga aggcgggatc ggcgattggg acgaagtcgt 1440
aacaagatgc ccccc 1455
Claims (9)
1. A strain characterized by: the strain is Modestobacter desetti CPCC D00004, and the preservation number of the strain in the China general microbiological culture Collection center is CGMCC No. 18004.
2. A bacterial agent, the active ingredient of which is the strain of claim 1.
3. Use of the strain of claim 1 or the microbial agent of claim 2 in any one of:
(A1) degrading organic phosphorus;
(A2) preparing products for degrading organic phosphorus.
4. Use according to claim 3, characterized in that: the organophosphorus is lecithin.
5. Use of the strain of claim 1 or the microbial agent of claim 2 in any one of:
(B1) preparing alkaline phosphatase;
(B2) preparing a product having alkaline phosphatase activity;
(C1) preparing acid phosphatase;
(C2) preparing a product having acid phosphatase activity.
6. Use of the strain of claim 1 or the microbial agent of claim 2 in any one of:
(D1) treating garbage;
(D2) soil remediation;
(D3) treating desert;
(D4) the soil quality is improved.
7. Any one of the following:
(E1) an organophosphorus degrading product produced by using the strain according to claim 1 or the microbial agent according to claim 2; the active component of the product is the strain;
(E2) a product having alkaline phosphatase activity, which is produced by using the strain according to claim 1 or the microbial agent according to claim 2; the active component of the product is the strain;
(E3) a product having acid phosphatase activity, which is produced by using the strain according to claim 1 or the microbial agent according to claim 2; the active component of the product is the strain.
8. The substance of claim 7, wherein: the organophosphorus is lecithin.
9. Use of the strain of claim 1 for the preparation of the microbial inoculum of claim 2.
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| CN106544303A (en) * | 2016-12-07 | 2017-03-29 | 南京农业大学 | One plant of efficient phosphate-solubilizing bacterium P18 for being isolated from cow dung compost and its application |
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