CN100377768C - Micro fluid chip - Google Patents
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- CN100377768C CN100377768C CNB2005100058164A CN200510005816A CN100377768C CN 100377768 C CN100377768 C CN 100377768C CN B2005100058164 A CNB2005100058164 A CN B2005100058164A CN 200510005816 A CN200510005816 A CN 200510005816A CN 100377768 C CN100377768 C CN 100377768C
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Abstract
The invention relates to a micro fluid chip that leads liquids supplied from a plurality of liquid supply ports, respectively, to a minute flow passage, performs mixing and reaction (chemical reaction) of the liquids in the minute flow passage, and obtains a liquid having been processed from a liquid discharge port. A micro fluid chip that leads liquids supplied from a plurality of liquid supply ports, respectively, to a minute flow passage, performs mixing/reaction of the liquids in the minute flow passage, and obtains a liquid having been processed from a liquid discharge port, the micro fluid chip comprising liquid supplies that supply a plurality of flows, which are formed by division of two kinds of liquids, respectively, in an alternate arrangement, and a flow flattening portion provided downstream of the liquid supplies to be configured in flow passage such that liquids alternately arranged are decreased in dimension as they go downstream and increased in dimension in a direction, which intersects the direction of arrangement and a direction of flow, as they go downstream, to be made substantially the same or slightly large in cross sectional area in the direction of flow. According to the invention, liquids of large flow rates can be processed at high speed and an apparatus is not made large in size.
Description
Technical field
The present invention relates to micro fluid chip, this micro fluid chip will import small stream from the liquid that a plurality of liquid supply ports are supplied with respectively, in small stream, carry out mixing, the reaction (chemical reaction) of liquid, then the liquid of handling from the liquid outlet acquisition.
Background technology
So-called micro fluid chip is that the sensing width and the degree of depth are to supply with multiple liquid the small stream from a few μ m to hundreds of μ m, according to the molecule that constitutes liquid, the automatic motion of particle, carries out the mixing of liquid, the device of reaction in small stream.
That is, in the small stream that small fluid chip had, the Reynolds number of liquid is below the hundreds of, does not carry out the sinuous flow control as reflection device in the past, but the environment of controlling for laminar flow.For liquid mixing, the reaction each other under this laminar flow control, mixing, reaction based on the molecular diffusion of the contact interface of each liquid are main, and the device that is used to limit this speed is the thickness (liquid not of the same race is diffusible distance=diffusion length till becoming the concentration of homogeneous) of the dispersal direction of liquid.
In micro fluid chip in the past, with the analysis of liquid is that the device of purpose is more, and these devices make in the small stream of narrow (=diffusion length is shorter) of the width of liquid below hundreds of μ m of the flow with a few μ l/min to tens μ l/min and mix, react.
Concrete is, it is known that to open the 2003-1077 communique just like the spy disclosed like that, by multiple liquid being divided into the liquid stream of a plurality of stratiforms respectively, and with its configuration that replaces, with this layering liquid stream of ratio that forms the contact area of each shared in the cumulative volume that can be increased in liquid liquid, can carry out the device of the mixing of liquid efficiently; Perhaps as the spy open the 2002-346355 communique disclosed, liquid is shaped to the membranaceous liquid stream of ripple, and it is vertically carried out with respect to the liquid flow path direction stacked, by this stacked liquid stream is pushed intermittently, with the device of the stirring stream mixing material that produces etc.
Above-mentioned technology in the past, be to be prerequisite with a spot of liquid of analyzing about tens μ l/min, design micro fluid chip, in order to carry out the mixing of liquid with the flow about tens μ l/min, reaction, if to make liquid flow at a high speed, then stream is too small causes the internal pressure loss excessive, the desirable flow that can not circulate can not be expected the increaseization for the treatment of capacity.In addition, if with high speed circulating liquid in small stream, then diffusion becomes insufficient, can not obtain desired mixing, reaction.
In order to increase treating capacity, to obtain practical mixing, reaction, be necessary to handle side by side, this processing arranged side by side is used and being called the parallel a plurality of micro fluid chips that amplify (numbering up), causes the maximization of device integral body.
For the pressure loss in the small stream of micro fluid chip, the viscosity of liquid has bigger influence power.This is because higher with respect to the ratio of the cross-sectional perimeters of stream sectional area, and the friction loss that causes the viscosity owing to the stream wall to cause is bigger.Therefore, if to micro fluid chip transfer of highly viscous liquid, then near wall, stagnation, accumulation that generation is caused by the pressure loss are difficult to carry out correct liquor charging.
Particularly, supply with low viscous liquid and full-bodied liquid and mix, when reacting, because the pressure loss of low-viscosity (mobile) liquid and high viscosity liquid is variant, even cause low-viscosity (mobile) liquid more successfully to flow through small stream, high viscosity liquid also will be stagnated.Therefore, even with desirable ratio feed fluid originally, because in the process of small stream of flowing through, flow produces difference, the amount of one side's fluid tails off with respect to the opposing party, so under the situation of mixing, can not carry out the mixing of desirable ratio, cause under situation about reacting, the income amount reduces.In addition, gradually, full-bodied liquid is stagnated in small stream, causes the most occupied by high viscosity liquid of stream, can not carry out desirable mixing, reaction.
Summary of the invention
Therefore the purpose of this invention is to provide a kind of micro fluid chip that can handle the liquid of big flow at high speed and device is maximized.
In addition, the purpose of this invention is to provide and a kind ofly can supply with multiple liquid that comprises high viscosity liquid and the micro fluid chip that can continue to carry out desirable mixing, reaction.
The feature of the micro fluid chip of the present invention that is used to achieve the above object is, to import small stream from the liquid that a plurality of liquid supply ports are supplied with respectively, in small stream, carry out mixing, the reaction of liquid, obtain the liquid handle from liquid outlet then, in such micro fluid chip, be provided with liquid supply unit and liquid stream flattening portion, the aforesaid liquid supply unit will be cut apart respectively and a plurality of liquid streams of forming are supplied with the form of alternately arranging at least 2 kinds of liquid; Above-mentioned liquid flows flattening portion, its stream is shaped as: in the downstream of this liquid supply unit, on this liquid supply unit, alternately the size of the orientation of the liquid of arranging diminishes gradually towards the downstream, the size of the direction that intersects with this orientation and flow direction becomes gradually towards the downstream greatly, and sectional area becomes big about equally or a little at the liquid flow path direction.
And then, the feature of the micro fluid chip of the present invention that is used to achieve the above object is, be provided with the stream handling part, its length is: can keep sectional area till liquid outlet with previous status, and can guarantee to finish the mixing of each liquid of supplying with from this liquid supply unit or the holdup time till the reaction, above-mentioned sectional area is meant in the downstream of this liquid stream flattening portion, with the sectional area of the connecting portion of this liquid stream plat part of the direction that intersects with liquid stream.
In this liquid supply unit, if size along with the orientation of the liquid that reduces gradually towards the downstream alternately to arrange, though can think alternately the narrowed width (diffusion length shortening) of each liquid in the orientation of the liquid arranged, can spread well, but, as if the size of the direction that keeps at the liquid flow path direction intersecting with this orientation and liquid flow path direction, then flow path cross sectional area diminishes at the liquid flow path direction.So, the pressure loss becomes the big flow that can not circulate greatly.
So, the size by increasing the direction that intersects with this orientation and liquid flow path direction at the liquid flow path direction, flow path cross sectional area is increased about equally or a little at the liquid flow path direction, can stop the pressure loss to increase, and the liquid of the big flow that circulates.
In this liquid supply unit, if size along with the orientation of the liquid that reduces gradually towards the downstream alternately to arrange, the narrowed width of each liquid of the orientation of the liquid of alternately arranging then, on this basis, if increase the size of the direction that intersects with this orientation and liquid flow path direction in the direction of liquid stream, then can enlarge the liquid flow path direction unit length in abutting connection with liquid contact area each other.
In ensuing handling part, if will with the intersect sectional area of the connecting portion that is connected with liquid stream flattening portion of direction of liquid stream, with previous status, remain to liquid outlet, even then because in handling part, also can keep liquid stream extended contact area each other, this liquid stream is meant flowing in abutting connection with liquid of unit length on the liquid flow path direction, so can shorten to the mixing of each liquid of supplying with from this liquid stream supply unit or the holdup time till the reaction terminating, because the length of the stream of handling part shortens, so can carry out the miniaturization of device.
Therefore,, liquid at high speed ground is flowed according to the present invention, in addition, because the rising of the flow that on 1 micro fluid chip, can handle, so can reduce number of processes arranged side by side, and can avoid the maximization of device.
In addition, the feature of the micro fluid chip of the present invention that is used to achieve the above object is, the liquid of supplying with respectively from a plurality of liquid supply ports of micro fluid chip is imported into inner small stream, in small stream, implement mixing, the reaction of liquid, obtain the liquid handle from liquid outlet, in such micro fluid chip, this liquid supply port is the device that is used for supplying with respectively at least 2 kinds of liquid, there are the guiding road of high viscosity liquid, the opening on this guiding road to be arranged in the liquid stream of low-viscosity (mobile) liquid in the stream upper shed of low-viscosity (mobile) liquid.
Therefore, according to the present invention, owing to around high viscosity liquid, have low viscous liquid, high viscosity liquid does not directly contact with the wall of small stream, so the stagnation of liquid stream can not take place in high viscosity liquid, can continue to have obtained the mixing of desirable liquid, the treatment fluid of reaction.
And, because the contact area of high viscosity and low-viscosity (mobile) liquid increases,, can carry out mixing, the reaction of desirable liquid efficiently so the mutual molecular diffusion of liquid can be carried out well.
Description of drawings
Fig. 1 is the summary exploded perspective view as the micro fluid chip of an embodiment of the invention;
Fig. 2 is the front view of micro fluid chip main body shown in Figure 1;
Fig. 3 is the transverse cross-sectional view of the micro fluid chip of the I～I line of cut along Fig. 2;
Fig. 4 is the part enlarged drawing of the layering liquid stream formation portion of micro fluid chip main body shown in Figure 1;
Fig. 5 is the part longitudinal sectional drawing of the micro fluid chip main body of the II～II line of cut along Fig. 2;
Fig. 6 is the figure that the liquid of micro fluid chip main body shown in Figure 1 stream flattening portion is described;
Fig. 7 is analog representation as the small stream of the handling part of micro fluid chip main body shown in Figure 1 and the figure of suitable shape;
Fig. 8 is analog representation as the small stream of the handling part of micro fluid chip main body shown in Figure 1 and the figure of other suitable shapes;
Fig. 9 is the summary exploded perspective view as the micro fluid chip of other embodiments of the present invention;
Figure 10 is the front view of micro fluid chip main body shown in Figure 9;
Figure 11 is the back view of cover shown in Figure 9;
Figure 12 is the transverse cross-sectional view of the micro fluid chip of the III～III line of cut along Figure 10;
Figure 13 is the transverse cross-sectional view of the micro fluid chip of the IV～IV line of cut along Figure 10;
Figure 14 is the part stereogram of flow condition that is used to illustrate the liquid of micro fluid chip main body shown in Figure 10;
Figure 15 is the part longitudinal sectional drawing of the micro fluid chip of the V～V line of cut along Figure 14;
Figure 16 is the part longitudinal sectional drawing of the micro fluid chip of the VI～VI line of cut along Figure 14;
Figure 17 is the longitudinal sectional drawing of the micro fluid chip main body of the VII～VII line of cut along Figure 10;
Figure 18 is the enlarged drawing of the variation of the expression second liquid discharge portion shown in Figure 9;
Figure 19 is the part longitudinal sectional drawing based on the micro fluid chip of Figure 15 of expression other embodiments of the present invention.
The specific embodiment
Below, illustrated embodiment is described.
Below, to as an embodiment of micro fluid chip of the present invention, the micro fluid chip that is used to mix 2 kinds of liquid illustrates and describes, but the present invention is not limited to these embodiments.
Fig. 1 is the summary exploded perspective view of the integral body of expression micro fluid chip 1.
As seal member, can use cementability liner component, metal gasket parts, but also can use other method direct fixed cap parts 30, adaptor member 50 on the table of micro fluid chip main body 10, inboard such as laser joints, cement.In addition, in Fig. 1, the ellipse that is depicted on micro fluid chip main body 10, cover 30, the adaptor member 50 is a screw hole.In addition, though on adaptor member 50 and seal member 90, be respectively arranged with opening, omit its detailed diagram as the path of the treatment fluid of the stoste of supplying with, discharge.
Fig. 2 is the front view of interarea one side of micro fluid chip main body 10, has the first liquid supply unit 11, the first liquid-conducting stream portion 12, the second liquid supply unit 13, layering liquid stream formation portion 14, liquid stream flattening portion 15, handling part 16, liquid discharge portion 17.
At this, the basin of stream formation portion 14 from the first liquid supply unit 11 to layering liquid is liquid supply units that a plurality of liquid streams that at least 2 kinds of liquid are split to form are respectively supplied with the form of alternately arranging; Handling part 16 is the mixing that is used to carry out liquid, the small stream of reaction (chemical reaction).
In micro fluid chip main body 10, be provided with groove from the following liquid supply unit that specifically describes to the different shape of liquid outlet, connect airtight the effect of the lid of lip-deep cover 30 these grooves of performance sealing that are fixed in micro fluid chip main body 10.
The first liquid supply unit 11 is made of a plurality of first liquid supply nozzle 11a.Each first liquid supply nozzle 11a is made of opening, and this opening leaves the form that certain compartment of terrain is arranged in row with the width in micro fluid chip main body 10 and is provided with from the back side (interareas of adaptor member 50 1 sides) of micro fluid chip main body 10 to surperficial (interareas of cover 30 1 sides).
Fig. 3 is micro fluid chip main body 10 transverse cross-sectional view of the I～I line of cut along Fig. 2, is provided with in the bottom (back sides of adaptor member 50 1 sides) of each first liquid supply nozzle 11a to be used for the temporary transient dashpot 18 that stores as first liquid of supplying with liquid.Dashpot 18 is formed by the recess and the adaptor member 50 at the back side that is arranged at micro fluid chip main body 10.
With the dashpot 18 corresponding positions of adaptor member 50 on first liquid be installed supply with 19, supply with 19 first liquid of supplying with from first liquid, after being full of dashpot 18, follow the rising of liquid level to supply with equably to the first all liquid discharge nozzle 11a.Its result discharges first liquid from the first all fluid injector 11a, and the first liquid-conducting stream portion 12 of these liquid by extending from each nozzle moves to layering liquid stream formation portion 14.
The second liquid supply unit 13, by constituting at stagger respectively a plurality of second liquid discharge nozzle 13a of a nozzle location of the width of micro fluid chip main body 10 with the first liquid discharge nozzle 11a, have dashpot, second liquid supply with first-class aspect, have identical structure with the first liquid supply unit 11.
Second liquid is discharged from the second all liquid discharge nozzle 13a, supplies with to layering liquid stream formation portion 14.
Shown in Fig. 4 amplifies, in the first liquid-conducting stream portion 12, have the first liquid-conducting stream 12b in the position from each first liquid discharge nozzle 11a between a plurality of wall 12a of portion of the length direction (flow direction of first liquid) of micro fluid chip main body 10, first liquid flows down from the first liquid-conducting stream 12b.
A plurality of second liquid discharge nozzle 13a are opened on the end of downstream side of the 12a of wall portion, two kinds of liquid from each first liquid discharge nozzle 11a and each second liquid discharge nozzle 13a discharge, because departing from of the position of two nozzle 11a, 13a, in layering liquid stream formation portion 14, form the layering liquid stream 14A that 2 kinds are disposed liquid alternatedly.
By forming layering liquid stream, the ratio of shared contact area increases in the cumulative volume of 2 kinds of liquid, the molecular diffusion that produces at contact-making surface comes to life, owing to increase the molecular diffusion amount of certain hour, so realized the high efficiency of the processing of micro fluid chip.Under the situation of same volume, the number of plies of the treatment effeciency of micro fluid chip and layering liquid stream is proportional.
The layering liquid stream 14A that forms in layering liquid stream formation portion 14 then moves to liquid stream flattening portion 15.
In the mixing of the liquid that is undertaken by molecular diffusion, what can have influence on time of mixing till finishing is and the thickness (diffusion length of=molecule, particle) of the liquid of the perpendicular direction of contact interface to have square proportional relation of time and thickness.For example, under situation, be 0.5mm if diffusion length is shortened 1/2 with the diffusion of carrying out 1mm in 10 seconds, then the time only needs 1/4 to be 2.5 seconds.
According to this relation, in liquid stream flattening portion 15, that dwindle layering liquid stream 14A and the flow path width perpendicular direction of contact interface.Thus, can shorten diffusion length, at full speed carry out the mixing of liquid.
In addition, before shortening with shorten after the width of stream than under the situation of identical value, if increase by the first liquid discharge nozzle 11a and at the width of micro fluid chip main body 10 columns of alternately arranging of the second liquid discharge nozzle 13a of each position that staggers, make the number of plies of layering liquid stream many more, then 1 layer of thickness attenuation on every side can shorten to the time of mixing till finishing thus.
Usually, if reduce the width of stream, then flow path cross sectional area reduces, and the pressure loss of the unit length in the stream becomes big towards the downstream thus.Particularly, to a large amount of conveying liquid of micro fluid chip the time, the pressure loss in the stream surpasses and is disposed at the not shown pump performance limit that first liquid is supplied with the upstream side of 19 grade, causes carrying liquid.
So, in the present embodiment, in the micro fluid chip main body 10 that the thin plate about 0.5mm commonly used is made, using the thick sheet material of several mm in the past, as shown in Figure 2 along with when the downstream dwindles flow path width W gradually, as shown in Figure 5, promptly, enlarge the depth H of stream with it pro rata, flow path cross sectional area is remained certain liquid stream flattening portion 15 so that the pressure loss is increased by being provided with as along shown in the part longitudinal sectional drawing of II shown in Figure 2～II line of cut.Liquid stream flattening portion 15 is also referred to as the diffusion length shortening portion with prevention pressure loss increase function.
In this case, even enlarge the depth H of stream, the liquid stream in the stream in order to keep laminar flow, also can not make layering liquid stream 14A entanglement, and molecular diffusion can be not disorderly yet.
As shown in Figure 6, in liquid stream flattening portion 15, with the direction of liquid stream as directions X, with width (being the orientation of the liquid alternately arranged in liquid supply unit 11) as the Y direction, under the situation that depth direction (in liquid supply unit 11 for the orientation of the liquid of alternately arranging and the direction that the liquid flow path direction intersects) is represented as the Z direction, 1 layer of each of layering liquid stream, thickness attenuation along with advanced downstream (thickness w1 → w2), alternately, (degree of depth h1 → h2) carries out flattening with elongated form at depth direction (Z direction), even make sectional area on the direction that intersects with the liquid flow path direction, still also can enlarge (the sectional area in downstream of sectional area S2 〉=S1) a little in handling part 16 1 sides (sectional area S2) roughly the same (sectional area S1 S2) in the layering liquid of upstream stream formation portion's 14 1 sides (sectional area S1) and downstream.
For with the section shape of the connecting portion that is connected 20 of handling part 16, can determine according to the thickness of micro fluid chip 10, the length of aftermentioned handling part 16.
In Fig. 2, though the semicircular in shape shape is seen from the front of micro fluid chip main body 10 by liquid stream flattening portion 15, shape is not limited, leg-of-mutton also passable.In addition, shapes such as semi-circular shape, triangle also can be simulated the step-like shape of fine pitch, and corresponding its shape also can be the step-like of gentle dip at depth direction.
Liquid by liquid stream flattening portion 15 moves to handling part 16.
Handling part 16 keeps sectional area with previous status till the liquid discharge portion (liquid outlet) 17, and this sectional area is meant the sectional area of the connecting portion 20 that is connected with the crisscross liquid stream flattening portion 15 of liquid stream.
This be because, by the stream section shape is unified, can process easily, in addition, can easily calculate the calculating of liquid admixture.But, if the thickness of micro fluid chip main body 10 has surplus, then can deepen the flow path depth H of liquid stream flattening portion 15, increase the ratio of interfacial area/whole volume of layering liquid stream 14A, can further improve mixed performance.
In embodiment, handling part 16 is done circular whirlpool shape, ask the holdup time that obtains mixing the necessity till the end by calculating, if can guarantee on micro fluid chip main body 10 from the flow path length of necessity of this guiding, then the shape of other of the square whirlpool shape of Fig. 7, not shown hexagonal whirlpool shape, the snakelike shape of Fig. 8 etc. is also passable.
The mixing of liquid finishes fully during by this handling part 16, from liquid discharge portion 17 to the mixed equably treatment fluid of the outer discharge of device.Be provided with not shown treatment fluid discharge head with the liquid discharge portion 17 corresponding positions of adaptor member 50, discharge head by this treatment fluid and obtain a treatment fluid.
So long as the liquid laminar flow, the mixed performance of micro fluid chip 1 of the present invention is by the number of plies of layering liquid stream 14A and the front and back flow path width decision of liquid stream flattening portion 15.Thus, may not need such fine nozzle, the groove of micro fluid chip in the past, even also can bring into play performance to the nozzle of several mm, the combination of groove, so excellent in workability owing to be hundreds of μ m respectively.
In the embodiment of Fig. 2, though layering liquid is flowed 14A as transversely arranged shape, but the shape of arranging at the thickness direction of micro fluid chip main body 10, promptly, each direction of principal axis for Fig. 6, can be that the center is revolved and turn 90 degrees with the direction of principal axis of Y and Z with X, make Y become the extended spread of the Z-direction of Fig. 6.
In the above-described embodiment, be illustrated, but under the situation that the liquid more than 3 kinds is mixed, the liquid supply unit can be set morely with the example that 2 kinds of liquid are mixed.
The embodiment of the micro fluid chip that 2 kinds of liquid that viscosity is different are mixed describes below.
Fig. 9, it is the summary exploded perspective view of expression micro fluid chip 1, micro fluid chip 1, by: micro fluid chip main body 10, this micro fluid chip main body 10 is used to mix, the processing of reaction etc., forms corresponding to the kind of the liquid thick sheet material of several mm by metal, glass, silicon, resin etc.; With cover 30, this cover 30 is disposed at an interarea one side of micro fluid chip main body 10, is used to constitute the top plate portion of the stream of micro fluid chip main body 10; With adaptor member 50, this adaptor member 50 is disposed at another interarea one side of micro fluid chip main body 10 of an opposite side of this cover 30, and the conveying mechanism and the chip of hitch pump etc.; And the seal member 70,90 (with reference to Figure 12, Figure 13) that is disposed between these 3 parts constitutes, and the binding of the not shown screw of these parts by running through 8 screw holes 2～4 around being arranged at prevents leak of liquid.
As seal member, can use cementability liner component, metal gasket parts, but also can use other method direct fixed cap parts 30, adaptor member 50 on the table of micro fluid chip main body 10, the inside such as laser joints, cement.
From stream one side of chip body 10 see the right side and below, as the location parts, be provided with the recessed guide part 11 of L word shape, on cover 30 as being provided with the protruding guide part 31 of L word shape with recessed guide part 11 corresponding parts, when linking chip body 10 and cover 30, by making up the jog of this guide part, can easily carry out correct positioning.
On adaptor member 50, be provided with opening 51～53, be arranged at and between chip body 10 and the adaptor member 50 be provided with same opening with opening 51～53 corresponding positions seal member 90 (with reference to Figure 12, Figure 13) as the path of the treatment fluid of the stoste of supplying with, discharge.As described later,, in opening 51, be connected with the head (stopper or joint) that is used to supply with low-viscosity (mobile) liquid, on opening 52, be connected with the head (stopper or joint) that is used to supply with high viscosity liquid in the rear side of adaptor member 50.
Figure 10 is the front view of interarea one side of micro fluid chip main body 10, has the oval-shaped first liquid supply unit 12, a plurality of second liquid supply unit 14, layering liquid stream formation portion 15, liquid stream flattening portion 16, handling part 17, liquid discharge portion 18.
In this embodiment, the basin of stream formation portion 15 from the first liquid supply unit 12 to layering liquid is liquid supply units that a plurality of liquid streams that at least 2 kinds of liquid are split to form are respectively supplied with the form of alternately arranging; Handling part 17 is the mixing that is used to carry out liquid, the small stream of reaction.
Discharge low-viscosity (mobile) liquid from the first liquid supply unit 12, discharge high viscosity liquid from the second liquid supply unit 14, the first liquid supply unit 12 is positioned at the upstream, and liquid discharge portion 18 is positioned at the downstream.
A plurality of second liquid supply units 14 with the form along the oval-shaped length direction of the first liquid supply unit 12, that is, are arranged according to the interval of desirable equalization with the form vertical with respect to the flow direction of low-viscosity (mobile) liquid.
Till the first liquid supply unit, 12 to second liquid supply units 14 is the stream 13 of low-viscosity (mobile) liquid.Even in layering liquid stream formation portion 15, liquid stream flattening portion 16, also slowly carry out mixing, the reaction of liquid.These structures be will be described hereinafter.
Figure 11 is the front view with the cover 30 of micro fluid chip main body 10 contacted sides, by constituting as a plurality of second liquid caps 32 of the lid of a plurality of second liquid supply units 14 with as other the stream cap 33 of lid of stream of micro fluid chip main body 10.Stream cap 33 is to form by the surface of cutting cover 30, than hanging down one section with the second liquid cap 32, contacted 34 of seal member 70 (with reference to Figure 12, Figure 13).In addition, seal member 70 has the shaped aperture of 8 screw holes 3, stream cap 33, and liquid contacts with stream cap 33.
In micro fluid chip main body 10, be provided with the groove of the following different shape that specifically describes from the liquid supply unit to liquid outlet, connect airtight the lip-deep cover 30 that is fixed in micro fluid chip main body 10 and realize the function of the lid of these slot parts of sealing, constitute above-mentioned each stream (13,15～17) by each groove and cover 30.
Figure 12 is the transverse cross-sectional view of micro fluid chip 1 of the position of the III～III line of cut along Figure 10; The first liquid supply unit 12 has the single oval nozzle as the first liquid outlet 12a.
First liquid is installed on the position corresponding with the first liquid outlet 12a of adaptor member 50 supplies with 5, supply with 5 first liquid (low-viscosity (mobile) liquid) A. that supply with from this first liquid and be full of the first liquid outlet 12a, form the laminar liquid that equates with the whole width of stream 13 shown in Figure 10 and flow along with the rising of liquid level.
Figure 13 is the transverse cross-sectional view of micro fluid chip 1 of the position of the IV～IV line of cut along Figure 10.
As shown in figure 13, the second liquid supply unit 14 is provided with a plurality of second liquid supply port 14a, each second liquid supply port 14a is made of opening, and above-mentioned opening leaves the form that certain compartment of terrain is arranged in row with the width in micro fluid chip main body 10 and is provided with from the back side (interareas of adaptor member 50 1 sides) of micro fluid chip main body 10 to surperficial (interareas of cover 30 1 sides).
In the back side portion of adaptor member 50 1 sides of micro fluid chip main body 10, be provided with the dashpot 19 that is used for temporarily storing second liquid (high viscosity liquid) B with the form that is communicated with each second liquid supply port 14a.Dashpot 19 is formed by the recess and the adaptor member 50 at the back side that is arranged at micro fluid chip main body 10.
With the dashpot 19 corresponding positions of adaptor member 50 on second liquid be installed supply with 6, supply with 6 second liquid B of supplying with from second liquid, after being full of dashpot 19, follow the rising of liquid level to supply with equably to the second all liquid outlet 14a.Its result discharges second liquid from all second liquid outlet 14a shuntings.
Figure 14 amplifies the major part of the face side of the first liquid supply unit, the 12～layering liquid stream formation portion 15 that represents micro fluid chip main body 10.
Around each second liquid supply port 14a, the protuberance 20 that is roughly assumed diamond in shape surrounds, each top flat, and contact has each second liquid cap 32 on this tabular surface.Each second liquid cap 32 has the shape identical with protuberance 20.On each protuberance 20, be provided with from the recess 21 of each second liquid supply port 14a one side towards the downstream, the bottom of recess 21 is positioned at than on the higher position, the position of stream 13, layering liquid stream formation portion 15.
Each second liquid supply port 14a is owing to seal by the second liquid cap 32 from outstanding one section of stream cap 33, so will collaborate from flow through first liquid A of stream 13 of each recess 21.Therefore, each second liquid supply port 14a and each recess 21 that is communicated with therewith constitute the guiding road of each second liquid B.The opening on this guiding road promptly, flows the opening 22 that forms portion 15 towards the layering liquid that is formed by each recess 21 and each second liquid cap 32, from the bottom of stream 13, the top board of stream cap 33 both leave.Therefore, each second liquid B is discharged to from opening 22 is flowed through the liquid stream of first liquid (low-viscosity (mobile) liquid) A of stream 13, and does not contact with the bottom of layering liquid stream formation portion 15, the top plate portion of stream cap 33.
Arrow with the direction of the liquid stream of representing each liquid shown in Figure 14 illustrates this state.In addition, in order to understand the liquid stream of each liquid, the second liquid cap 32 of an expression part easily.
Low viscous first liquid A of discharging from the first liquid supply port 12a is liquid stream in the form of sheets, is full of stream 13 integral body on one side and moves, and contacts with each protuberance 20 of the second liquid cap 32 and the second liquid discharge portion 14.Because this sheet liquid stream is laminar flow, so after the contact, do not shunt with the form that wraps into the second liquid cap 32 and each protuberance 20 mussily shown in the arrow among the figure, collaborates once more in the downstream of each protuberance 20.
As the longitudinal sectional drawing along V～V line of cut of above-mentioned Figure 14, promptly shown in Figure 15, each opening 22 of discharging second liquid B exceeds one section from the stream bottom surface, and the second liquid cap 32 is from outstanding one section of stream top board.Therefore, high viscosity liquid (second liquid) B from each opening 22 is discharged is discharged in the liquid stream of first liquid A of collaborating in the downstream of each protuberance 20, and advances to layering liquid stream formation portion 15.
From each opening 22 discharges second liquid B the time, owing to flowed in the surrounding low viscous first liquid A, so as along the transverse cross-sectional view of VI～VI line of cut of Figure 14, promptly shown in Figure 16, full-bodied second liquid B becomes the liquid stream that the whole body is surrounded by first liquid A, and full-bodied second liquid B does not contact with the stream wall.
Thus, can prevent the increase that contacts the pressure loss that causes owing to wall and full-bodied liquid, and stagnation, the accumulation of the high viscosity liquid that accompanies therewith, can carry out correct liquor charging.At this moment, send the diameter of the second liquid supply port 14a of high viscosity liquid, if liquid stream is then in order to suppress the pressure loss of the second liquid supply port 14a, preferably bigger for laminar flow.
Owing to the liquid stream identical with each second liquid supply port 14a quantity of the second liquid discharge portion 14 can be arranged side by side, the described second liquid discharge portion 14 is arranged in row at the width of micro fluid chip main body 10, so as Figure 14, shown in Figure 16, in layering liquid stream formation portion 15, be formed with the layering liquid stream that first liquid and second is arranged liquid alternatedly.That is, the reason of layering liquid stream formation portion 15 being set, is the stable layering liquid stream of first liquid and second liquid alternately being arranged in order to obtain.
This layering liquid stream is for surrounding the shape of second liquid B after the shunting by first liquid A, can obtain to be increased in the effect of the ratio of contact area shared in the cumulative volume of 2 kinds of liquid.This result, the molecular diffusion that produces at contact-making surface is come to life, owing to increase the molecular diffusion amount of certain hour, realized the high efficiency (under the situation of same volume, the number of plies of the treatment effeciency of micro fluid chip and layering liquid stream is proportional) of micro fluid chip.
The layering liquid stream that forms in layering liquid stream formation portion 15 then moves to liquid stream flattening portion 16.
As longitudinal sectional drawing, promptly shown in Figure 17 in liquid stream flattening portion 16 along VII shown in Figure 10～VII line of cut, enlarge the degree of depth that layering liquid flows, dwindle flow path width.
In this case, identical with the liquid stream flattening portion 15 of Fig. 2, even sectional area on the direction that intersects with the liquid flow path direction is to keep (with reference to Fig. 6) necessarily (sectional area S1 S2) in the also roughly the same form of handling part 17 sides (sectional area S2) in upstream layering liquid stream formation portion's 15 sides (sectional area S1) and downstream with flow path cross sectional area.
Thus, even enlarge the depth H of stream, the liquid road in the stream also can keep laminar flow, and can not make layering liquid stream 13a entanglement, and molecular diffusion can be not disorderly yet.Its result, the pressure loss can not increase, and diffusion length shortens, and can carry out the mixing of liquid at high speed.That is, liquid stream flattening portion 16 stops the pressure loss to increase the diffusion length shortening portion of function for having.
Liquid stream flattening portion 16 is preferably the form that sectional area downstream enlarges gradually (sectional area S2 〉=S1).The section shape of liquid stream flattening portion 16 can determine according to the thickness of micro fluid chip main body 10, the length of aftermentioned handling part 17 etc.In Figure 10, though the semicircular in shape shape is seen from the front of micro fluid chip main body 10 by liquid stream flattening portion 16, shape is not limited, leg-of-mutton also passable.In addition, low the grade under the fully little situation of Reynolds number of flow velocity of liquid, shapes such as semi-circular shape, triangle also can be simulated the step-like shape of fine pitch, and corresponding its shape also can be the step-like of gentle dip at depth direction.
Liquid by liquid stream flattening portion 16 moves to handling part 17.
Handling part 17, will with the intersect width and the degree of depth of the connecting portion that is connected with liquid stream flattening portion 16 of direction of liquid stream, with previous status, remain to liquid discharge portion (liquid outlet) 18.
This be because, by the stream section shape is unified, can process easily, in addition, can easily calculate the calculating of liquid admixture.But, if the thickness of micro fluid chip main body 10 has surplus, then can deepen the flow path depth H of liquid stream flattening portion 16, increase the ratio of the interfacial area/whole volume of layering liquid stream, can further improve mixed performance.
The mixing of liquid finishes fully during by this handling part 17, from liquid discharge portion 18 to the mixed equably treatment fluid of the outer discharge of device.Be provided with not shown treatment fluid discharge head with the liquid discharge portion 18 corresponding positions of adaptor member 50, discharge head by this treatment fluid and obtain a treatment fluid.
In the present embodiment, wrap into high viscosity liquid with low-viscosity (mobile) liquid, improving outside the interfacial area rate, it can also be carried out layering liquid fluidisation and clamp-on, therefore, in handling part 17, compare with the mixing of being undertaken by common simple layering liquid stream that two kinds of liquid are alternately arranged, can carry out liquid with higher efficient and mix.
In addition, because high viscosity liquid stream is in the way of flowing, can be owing to the pressure loss is stagnated, and the ratio of high viscosity liquid and low-viscosity (mobile) liquid does not change at upstream side and downstream, therefore, can obtain the intact treatment fluid of reaction of the treatment fluid of desirable blending ratio, desirable amount constantly.
In the micro fluid chip 10 of above-mentioned embodiment, can pass through single chip, comprise the high speed of multiple liquid of high viscosity liquid and the mixing and the reaction of homogeneous with the processing flow of tens ml/min.
In addition, owing to form the bigger discharge nozzle of a plurality of easy processing, and formation will wrap into a plurality of liquid streams layering liquid stream of arranging arranged side by side of high viscosity liquid with low-viscosity (mobile) liquid, and can realize mixing, reaction at a high speed, so can not need the Micrometer-Nanometer Processing Technology ground of height to make micro fluid chip by pushing this liquid stream.
In an embodiment, though handling part 17 is made rectilinear form, but in order to ask the holdup time that obtains mixing the necessity till finishing by calculating, and on micro fluid chip main body 10, guarantee from the length of the necessary stream of its importing, also can adopt other shape such as whirlpool shape shown in Figure 2, serpentine shaped shown in Figure 8.
So long as the liquid laminar flow, the mixed performance of micro fluid chip 1 is by the number of plies of layering liquid stream and the front and back flow path width decision of liquid stream flattening portion 16.Thus, may not need such fine nozzle, the groove of micro fluid chip in the past, even also can bring into play performance to the nozzle of several mm, the combination of groove, so excellent in workability owing to be hundreds of μ m respectively.
In the embodiment of Figure 10, though layering liquid is flowed as transversely arranged shape, but the shape of arranging at the thickness direction of micro fluid chip main body 10, promptly, each direction of principal axis for Figure 10, can be that the center is revolved and turn 90 degrees with the direction of principal axis of Y and Z with X, make Y become the extended spread of the Z-direction of Figure 10.
In the above-described embodiment, though be illustrated with the example that 2 kinds of liquid are mixed, but under the situation that the liquid more than 3 kinds is mixed, can only append the second liquid supply unit 14 of amount of fluid in the downstream and be equivalent to the device of the corresponding with it second liquid cap 32.By the minimum liquid of the first liquid supply unit, 12 transport viscosity, next carry out liquor charging in the employed at this moment liquid according to low viscous order.
Figure 18 represents other the protuberance 20 of each two liquid outlet 14a of embodiment of the present invention.
In this embodiment, cover 30 is not for existing each second liquid cap 32 shown in Figure 11, the tabular of stream cap 33, protuberance 20 has the height that arrives cover 30, and the upstream side of the second outlet 14a of 20a is provided with the inclined plane 24 towards the bottom of recess 21 to the downstream above the top of protuberance 20.If flat cover 30 is installed at this, promptly shown in Figure 19 then as based on the longitudinal sectional drawing of the part of the micro fluid chip of Figure 15, by inclined plane 24 around the second liquid outlet 14a, and cover 30 between form gap K.
At this, if carry low viscous first liquid A by the first liquid supply unit 12, then shown in the arrow among the figure, at first contact with the upstream side front end of protuberance 20 and about shunting.Though the part of the liquid after the shunting moves to layering liquid stream formation portion 15 with previous status, but another part passes through gap K between inclined plane 24 and cover 30, move with the top form that covers recess 21, after forming the top board stream that forms by first liquid, form portion 15 and move to layering liquid stream.
Under this state, if second liquid B of transfer of highly viscous, then can form with low viscous first liquid surround full-bodied second liquid B around liquid stream, contact with the stream wall by high viscosity liquid B, can prevent the increase of the pressure loss that produces.
With situation that embodiment shown in Figure 9 is compared under, in the present embodiment because cover 30 can be smooth plate, so can omit the processing that is used for forming overleaf the second liquid cap 32, stream cap 33, productivity excellence.In addition, owing to do not need second cap 32 is correctly fitted with protuberance 20, when assembling, locate easily.
In addition, in the present embodiment, flow, can set arbitrarily so be used to realize the shape on its inclined plane 24 owing to can form the top board that forms by first liquid, described first liquid moves with the top form that covers recess 21 by the gap K between inclined plane 24 and the cover 30.
Claims (5)
1. micro fluid chip, to import small stream from first liquid of first liquid supply unit supply and second liquid of supplying with from the second liquid supply unit, in small stream, carry out mixing, the reaction of first and second liquid, obtain the liquid of handling from liquid outlet
The above-mentioned first liquid supply unit has, and low viscous first liquid is cut apart and a plurality of first liquid supply ports of a plurality of liquid stream that forms; The above-mentioned second liquid supply unit has in the downstream of the first liquid supply unit, contrast that the second high liquid of first liquid viscosity is cut apart and a plurality of second liquid supply ports of supplying with, above-mentioned a plurality of first and second liquid supply port is flowing alternately arrangement on the direction of intersecting with liquid
Downstream at the above-mentioned second liquid supply unit, be provided with liquid stream flattening portion, the stream of described liquid stream flattening portion is shaped as: above-mentioned first and the size of the orientation of second liquid supply port along with diminishing gradually towards the downstream more, the size of the direction that intersects with the both sides of this orientation and flow direction is along with becoming big gradually towards the downstream more, sectional area becomes big about equally or a little at the liquid flow path direction
On the stream of low viscous first liquid flow that above-mentioned first liquid supply port is supplied with, the guiding road of full-bodied second liquid that above-mentioned second liquid supply port is supplied with is towards downstream opening,
Above-mentioned micro fluid chip by tabular micro fluid chip main body, respectively with the table of this micro fluid chip main body in each side interarea airtightly fluid-tight engagement cover, and adaptor member constitute,
On the interarea of the table side of above-mentioned micro fluid chip main body, form the stream that constitutes above-mentioned first liquid flow and the groove of the small stream of binding with it,
Above-mentioned cover to be covering the form fluid-tight engagement of above-mentioned groove,
On groove, form protuberance as the stream of above-mentioned first liquid,
When above-mentioned second liquid supply port is communicated with the rear side of above-mentioned micro fluid chip main body, be formed on this protuberance,
The recess that is connected with this second liquid supply port is towards the formation downstream of the stream of first liquid flow,
Above-mentioned cover covers second liquid supply port and recess, and forms the guiding road of second liquid,
The top part of this cover one side of this protuberance is provided with the inclined plane, and this inclined plane is towards the downstream of the stream of first liquid and tilt, and and this cover between form the space.
2. micro fluid chip as claimed in claim 1, it is characterized in that, the downstream of flowing flattening portion at above-mentioned liquid is connected with handling part, in this handling part, till the aforesaid liquid discharge portion, keep flowing the sectional area of the connecting portion of plat part with previous status, and have mixing or the length of the holdup time till the reaction end that to guarantee up to first and second liquid of supplying with from the above-mentioned first and second liquid supply unit with liquid.
3. micro fluid chip as claimed in claim 1 is characterized in that, the flat shape that above-mentioned liquid flows flattening portion is semicircle shape or triangle, and the handling part that constitutes small stream is set to whirlpool shape or meander-like.
4. micro fluid chip as claimed in claim 1 is characterized in that, on the adaptor member at the back side that is arranged at above-mentioned micro fluid chip main body, is provided with and supplies with the second supply head that first of first liquid is supplied with head and supplied with second liquid.
5. micro fluid chip as claimed in claim 1, it is characterized in that, the guiding road of above-mentioned full-bodied second liquid and the path direction quadrature and the compartment of terrain of low viscous first liquid are provided with, thus first liquid and the second liquid alternated landform component layer liquid stream.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004230055 | 2004-08-06 | ||
| JP2004230055A JP4367283B2 (en) | 2004-08-06 | 2004-08-06 | Microfluidic chip |
| JP2004293179 | 2004-10-06 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1730142A CN1730142A (en) | 2006-02-08 |
| CN100377768C true CN100377768C (en) | 2008-04-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100058164A Expired - Fee Related CN100377768C (en) | 2004-08-06 | 2005-01-27 | Micro fluid chip |
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| Country | Link |
|---|---|
| JP (1) | JP4367283B2 (en) |
| CN (1) | CN100377768C (en) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007252979A (en) * | 2006-03-20 | 2007-10-04 | National Institute Of Advanced Industrial & Technology | Method for manufacturing compound by micro-reactor, its micro-reactor and distributor for micro-reactor |
| JP4940756B2 (en) * | 2006-05-22 | 2012-05-30 | ソニー株式会社 | Micro channel system |
| JP2008014791A (en) * | 2006-07-05 | 2008-01-24 | Nipro Corp | Liquid mixing device, liquid mixing method, and measuring method of very small amount of specimen |
| JP5030520B2 (en) * | 2006-09-29 | 2012-09-19 | 富士フイルム株式会社 | Fluid mixing method and microdevice |
| JP5221287B2 (en) * | 2007-12-27 | 2013-06-26 | 日揮触媒化成株式会社 | Method for producing barium titanate and barium calcium titanate using a microreaction apparatus |
| JP5470642B2 (en) * | 2008-03-25 | 2014-04-16 | 国立大学法人 岡山大学 | Micro droplet preparation device |
| JP2009233514A (en) * | 2008-03-26 | 2009-10-15 | Hitachi Plant Technologies Ltd | Microchemical reaction device and microchemical reaction system |
| JP5092881B2 (en) * | 2008-05-13 | 2012-12-05 | ソニー株式会社 | Channel structure and microchip |
| US20120003122A1 (en) * | 2009-03-20 | 2012-01-05 | Avantium Holding B.V. | Flow controller assembly for microfluidic applications and system for performing a plurality of experiments in parallel |
| JP5959835B2 (en) * | 2011-12-05 | 2016-08-02 | 株式会社日立製作所 | Reaction system |
| CN102698672B (en) * | 2012-05-21 | 2014-01-29 | 中南大学 | Microfluidic reactor |
| US10578613B2 (en) * | 2014-09-01 | 2020-03-03 | Ridgeview Instruments Ab | Solid support for improved detection of interaction between species |
| WO2018190336A1 (en) * | 2017-04-10 | 2018-10-18 | 古河電気工業株式会社 | Liquid delivery device and liquid delivery method |
| CN107702973B (en) * | 2017-09-08 | 2024-07-26 | 深圳市太赫兹科技创新研究院有限公司 | Whole blood plasma separation system and method |
| TWI651074B (en) * | 2017-10-25 | 2019-02-21 | 台達電子工業股份有限公司 | Mixing method and mixing apparatus for particle agglutination |
| WO2020118487A1 (en) | 2018-12-10 | 2020-06-18 | 深圳大学 | Micro-structure mold core of microfluidic chip and manufacturing method therefor |
| CN111829822B (en) * | 2019-04-18 | 2023-10-20 | 中国科学院微电子研究所 | Liquid chip sampling device of electron microscope |
| CN109967149B (en) * | 2019-04-24 | 2020-07-28 | 中国农业大学 | Micro-fluidic chip |
| CN110052297B (en) * | 2019-04-26 | 2021-08-27 | 广州迈普再生医学科技股份有限公司 | Microfluidic chip for fluid blending and multi-component fluid blending method |
| CN113720834B (en) * | 2021-08-25 | 2023-08-18 | 中国科学院南海海洋研究所 | Microfluidic chip, system and method for detecting biochemical elements of water body |
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| US5957579A (en) * | 1997-10-09 | 1999-09-28 | Caliper Technologies Corp. | Microfluidic systems incorporating varied channel dimensions |
| CN2596363Y (en) * | 2002-12-20 | 2003-12-31 | 上海博昇微晶科技有限公司 | Micro fluid system |
| EP1415708A2 (en) * | 2002-11-01 | 2004-05-06 | Hitachi, Ltd. | Chemical reactor and apparatus |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US5957579A (en) * | 1997-10-09 | 1999-09-28 | Caliper Technologies Corp. | Microfluidic systems incorporating varied channel dimensions |
| EP1415708A2 (en) * | 2002-11-01 | 2004-05-06 | Hitachi, Ltd. | Chemical reactor and apparatus |
| CN2596363Y (en) * | 2002-12-20 | 2003-12-31 | 上海博昇微晶科技有限公司 | Micro fluid system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1730142A (en) | 2006-02-08 |
| JP4367283B2 (en) | 2009-11-18 |
| JP2006043617A (en) | 2006-02-16 |
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