CN100367033C - Microflow control chip integrated unit in integrated constant temperature system in high efficiency - Google Patents
Microflow control chip integrated unit in integrated constant temperature system in high efficiency Download PDFInfo
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- CN100367033C CN100367033C CNB2005100390003A CN200510039000A CN100367033C CN 100367033 C CN100367033 C CN 100367033C CN B2005100390003 A CNB2005100390003 A CN B2005100390003A CN 200510039000 A CN200510039000 A CN 200510039000A CN 100367033 C CN100367033 C CN 100367033C
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Abstract
The present invention relates to a micro-flow control chip integral device in an integrated high-efficiency constant-temperature system, which comprises a thermostat and a miniature three-dimensional regulator, wherein the miniature three-dimensional regulator is fixed at one side of a pedestal platform; the thermostat is composed of an upper miniature metal thermotank and a lower miniature metal thermotank, a circulation pipeline for temperature control working substances is arranged in each metal thermotank, a micro-flow control chip is placed on a platform between the upper miniature metal thermotank and the lower miniature metal thermotank, a nut for support and screw adjustment is arranged between the two miniature thermotanks, and thus, the height between the two miniature thermotanks is equivalent to the thickness of the chip. The present invention is the micro-flow control chip integral device of the integrated thermostat. By using the device, an experiment can be carried out at set temperature, the influence of the thermal perturbation inside the system and outside the system on a microanalysis system is eliminated, the experimental reproducibility is greatly enhanced, and the application and the research range of electrophoretic chips are widened.
Description
Technical field
The present invention is a kind of micro-fluidic chip integrated apparatus of integrated efficient constant temperature system, specifically, be that thermostat directly is integrated in the capillary electrophoresis chip system, the advantage of the efficient constant temperature of performance thermostat, this integrated system is applicable to various chip detection technology (as optical detection, Electrochemical Detection and Mass Spectrometer Method etc.), belongs to chemical field.
Background technology
Micro-total analysis system is one of research forward position focus of present analysis science.Micro-total analysis system is with laboratory The whole analytical process such as sample preparation, sample filtering, sample separation so that sample detection etc. are integrated in carries out on the little chip block, can realize the robotization and the microminiaturization of The whole analytical process.
Micro-total analysis system that important focus is exactly the integrated electrophoresis chip of research in the micro-total analysis system field.At present, the chip electrophoresis analysis is generally at room temperature carried out.Lack attemperating unit, on the one hand, limited the analyst coverage of electrophoresis chip, as: need under specified temp, react and separate; On the other hand, variation of temperature can influence the reappearance and the reliability of experimental result.Therefore, improve the chip electrophoresis Controllable Temperature, can further widen the research field of electrophoresis chip, can improve the reappearance and the detection sensitivity of experimental result simultaneously.
Summary of the invention
The present invention seeks to: the micro-fluidic chip integrated apparatus that has proposed the integrated efficient constant temperature system.
The present invention also aims to provide that a kind of volume is small and exquisite, the integrated efficient constant temperature or the temperature-controlling system of constant temperature or convenient temperature control, reliable operation, this device can be by the selection of circulation fluid, realize temperature controlling in the microchannel, thereby the influence of research temperature to separating, the while can greatly be improved the reappearance and the sensitivity of experiment.
The technical solution adopted in the present invention is: the micro-fluidic chip integrated apparatus of integrated efficient constant temperature system, comprise a micro constant-temperature device, and a miniature three-dimensional regulator is formed.At the fixing miniature three-dimensional regulator of a side of base platform, regulator is clamped working electrode, can accurately locate, and carries out the styletable Electrochemical Detection.Thermostat is by two micro metal constant temperature ovens up and down, and the micro metal constant temperature oven is provided with temperature control working medium circulating line.Place micro-fluidic chip on the platform between the micro metal constant temperature oven up and down, be provided with between two micro metal constant temperature ovens and support and the adjustable diameter and screw adjusting nut, make between two micro metal constant temperature ovens highly suitable with chip thickness.Described micro metal constant temperature oven is by two square miniature copper (or aluminium) system constant temperature oven, also can be that steel, the stainless steel and other metal materials that surface anticorrosion is handled made.
Characteristics of the present invention are, the present invention is the micro-fluidic chip integrated apparatus of integrated thermostat, be provided with electric heater in the micro metal constant temperature oven or in the outside temperature control working medium container, can carry out accurate temperature controlling to temperature control working medium by PID modes such as (adjustings of infinitesimal analysis ratio), the mode by pump or natural convection makes the circulation of temperature control working medium.Use this device that experiment is carried out under the temperature of a setting, eliminate the influence of system internal and external temperature disturbance, improved the reappearance of experiment greatly, and widened the application and the research range of electrophoresis chip micro-analysis system.This apparatus platform becomes one constant temperature, separation, detection three segment sets, and Controllable Temperature is good, microminiaturized degree height.
Description of drawings
Fig. 1 is the micro-fluidic chip Electrochemical Detection integrated apparatus perspective diagram of integrated efficient constant temperature system of the present invention
Fig. 2 is the micro-fluidic chip Electrochemical Detection integrated apparatus schematic cross-section of integrated efficient constant temperature system of the present invention
Fig. 3 is the micro-fluidic chip Electrochemical Detection integrated apparatus schematic top plan view of integrated efficient constant temperature system of the present invention
Among the figure: 1, square micro constant-temperature case; 2, micro-fluidic chip; 3, the miniature three-dimensional regulator; 4, the inlet of constant temperature system and liquid outlet; 5, regulate nut highly between the two micro constant-temperature casees; 6, working electrode; 7, electrolytic cell; 8, base; 9, electrophoresis high-field electrode hole (anode of separation, sample introduction anode and sample introduction negative terminal are arranged); 10, the working electrode hole; 11, electrophoresis high-field electrode hole (separation negative terminal); 12, to electrode hole; 13, the contrast electrode hole.
Embodiment
Shown in Fig. 1,2 and 3, the micro-fluidic chip integrated apparatus of making integrated efficient constant temperature system provided by the invention (is example with the Electrochemical Detection) comprises following 3 steps: (1) at first makes constant temperature system platform and electrolytic cell (1 two square micro constant-temperature casees, the feed liquor of 4 constant temperature systems and fluid hole, 5 regulate the nut of height between the two micro constant-temperature casees, 7 electrolytic cells, 8 bases, 9 electrophoresis high-field electrode holes (separating anode, sample introduction anode and sample introduction negative terminal), 11 electrophoresis high-field electrode holes (separation negative terminal), 12 pairs of electrode holes, 13 contrast electrode holes); (2) again at the fixing miniature three-dimensional regulator (3 miniature three-dimensional regulators, 6 working electrodes) of base opposite side; (3) on square hollow microneedle constant temperature oven inner platform, place chip (2 micro-fluidic chip).
The constant temperature system platform adopts copper (or aluminium) matter metal material, and the micro constant-temperature case is square, and chip is on platform between the constant temperature oven, and the height that utilizes nut to regulate between the constant temperature oven makes it suitable with chip thickness.Be provided with electric heater in the metal constant temperature oven or in the outside temperature control working medium container, can carry out accurate temperature controlling to temperature control working medium by modes such as PID, the mode by pump or natural convection makes the circulation of temperature control working medium.Experimentation: earlier the micro-fluidic chip Electrochemical Detection integrated apparatus of integrated efficient constant temperature system is assembled by above-mentioned steps; Utilize the miniature three-dimensional regulator that the separating pipe endpiece of working electrode and micro-fluidic chip is accurately located at microscopically again, and, guarantee that chip is under the isoperibol with highly just in time identical between two micro constant-temperature casees of nut adjusting with chip thickness; Open the circulation fluid of constant temperature system then, regulate required experimental temperature and carry out constant temperature.Put three electrodes (working electrode, contrast electrode and to electrode) of electrophoresis high-field electrode (separate anode, separate negative terminal, sample introduction anode and sample introduction negative terminal) and Electrochemical Detection at last in the relevant position, carry out sample introduction, separation and Electrochemical Detection.
The micro-fluidic chip integrated apparatus of making the integrated efficient constant temperature system can carry out (being example with galvanochemistry) as follows: at first make constant temperature system platform and electrolytic cell; Again at the fixing miniature three-dimensional regulator of base opposite side; Placing micro-fluidic chip on the platform between two square micro constant-temperature casees at last, and the height of regulating between two square micro constant-temperature casees with nut makes it suitable with chip thickness, accurately locate working electrode (electrophoresis high-field electrode, to electrode, contrast electrode) with the miniature three-dimensional regulator, regulator can accurately be located working electrode, carries out the styletable Electrochemical Detection.
Claims (3)
1. the micro-fluidic chip integrated apparatus of integrated constant temperature system, comprise a thermostat, a miniature three-dimensional regulator, at the fixing miniature three-dimensional regulator of a side of base platform, the miniature three-dimensional regulator is clamped working electrode, it is characterized in that thermostat is made of two micro metal constant temperature ovens up and down, the micro metal constant temperature oven is provided with temperature control working medium circulating line, place micro-fluidic chip on the platform between the micro metal constant temperature oven up and down, be provided with between two micro metal constant temperature ovens and support and the adjustable diameter and screw adjusting nut, make between two micro constant-temperature casees highly suitable with chip thickness.
2. by the micro-fluidic chip integrated apparatus of the described integrated constant temperature system of claim 1, it is characterized in that described micro metal constant temperature oven is the square copper or the micro constant-temperature case of aluminum; Or the micro constant-temperature case of the steel or stainless steel metal material system of surface anticorrosion processing.
3. by the micro-fluidic chip integrated apparatus of the described integrated constant temperature system of claim 1, it is characterized in that being provided with electric heater in the micro metal constant temperature oven or in the outside temperature control working medium container that is provided with, the micro metal constant temperature oven is provided with pump and makes the circulation of temperature control working medium.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100390003A CN100367033C (en) | 2005-04-21 | 2005-04-21 | Microflow control chip integrated unit in integrated constant temperature system in high efficiency |
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| CNB2005100390003A CN100367033C (en) | 2005-04-21 | 2005-04-21 | Microflow control chip integrated unit in integrated constant temperature system in high efficiency |
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| CN1687770A CN1687770A (en) | 2005-10-26 |
| CN100367033C true CN100367033C (en) | 2008-02-06 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103184249A (en) * | 2011-12-31 | 2013-07-03 | 浙江工业大学 | Method for on-line synthesizing glucose-6-palmitate by lipase catalysis |
| CN103184256A (en) * | 2011-12-31 | 2013-07-03 | 浙江工业大学 | Method for on-line synthesizing saccharose-6-laurate by lipase catalysis |
| CN103184251A (en) * | 2011-12-31 | 2013-07-03 | 浙江工业大学 | Method for on-line synthesizing glucose-6-acetate catalyzed by lipase |
| CN103184255A (en) * | 2011-12-31 | 2013-07-03 | 浙江工业大学 | Method of using lipase to catalyze and synthesize galactose-6-acetate on line |
| CN103184250A (en) * | 2011-12-31 | 2013-07-03 | 浙江工业大学 | Method for on-line synthesizing mannose-6-acetate by lipase catalysis |
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| CN103182277B (en) * | 2011-12-31 | 2015-08-26 | 浙江工业大学 | Microfluidic channel reactor and the application in synthesis of sucrose-6-palmitate thereof |
| CN105548587A (en) * | 2015-12-02 | 2016-05-04 | 江苏理工学院 | Multifunctional micro-fluidic intelligent experimental device and working method thereof |
| CN105498870A (en) * | 2015-12-02 | 2016-04-20 | 江苏理工学院 | Micro-fluidic intelligent experimental device with reagent sampling function and working method thereof |
| CN106861779A (en) * | 2017-02-20 | 2017-06-20 | 广东工业大学 | A kind of segmented micro-fluidic chip temperature regulating device |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103184249A (en) * | 2011-12-31 | 2013-07-03 | 浙江工业大学 | Method for on-line synthesizing glucose-6-palmitate by lipase catalysis |
| CN103184256A (en) * | 2011-12-31 | 2013-07-03 | 浙江工业大学 | Method for on-line synthesizing saccharose-6-laurate by lipase catalysis |
| CN103184251A (en) * | 2011-12-31 | 2013-07-03 | 浙江工业大学 | Method for on-line synthesizing glucose-6-acetate catalyzed by lipase |
| CN103184255A (en) * | 2011-12-31 | 2013-07-03 | 浙江工业大学 | Method of using lipase to catalyze and synthesize galactose-6-acetate on line |
| CN103184250A (en) * | 2011-12-31 | 2013-07-03 | 浙江工业大学 | Method for on-line synthesizing mannose-6-acetate by lipase catalysis |
| CN103184251B (en) * | 2011-12-31 | 2014-11-12 | 浙江工业大学 | Method for on-line synthesizing glucose-6-acetate catalyzed by lipase |
| CN103184249B (en) * | 2011-12-31 | 2014-11-12 | 浙江工业大学 | Method for on-line synthesizing glucose-6-palmitate by lipase catalysis |
| CN103184256B (en) * | 2011-12-31 | 2014-11-12 | 浙江工业大学 | Method for on-line synthesizing saccharose-6-laurate by lipase catalysis |
| CN103184255B (en) * | 2011-12-31 | 2015-01-28 | 浙江工业大学 | Method of using lipase to catalyze and synthesize galactose-6-acetate on line |
| CN103184250B (en) * | 2011-12-31 | 2015-01-28 | 浙江工业大学 | Method for on-line synthesizing mannose-6-acetate by lipase catalysis |
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| CN1687770A (en) | 2005-10-26 |
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