CN100350928C - Cerebral apoplexy treating effervescence tablet and its preparation method - Google Patents

Abstract

The present invention relates to a Chinese medicine effervescent tablet for treating cerebral apoplexy, namely a cerebral embolism effervescent tablet, and a preparation method thereof. The preparation method adopts a direct tablet pressing method to press the effervescent tablet and the prescription of the cerebral embolism effervescent tablet contains cerebral embolism dry extract, crospovidone, mannitol, anhydrous citric acid, sodium bicarbonate, microcrystalline cellulose, silica gel powder, magnesium stearate, talcum powder and aspartame. The pressure range of a plain tablet is from 5.5N to 12.5N, disintegration time can reach 2'12', and effervescent time can reach 3'50'. The plain tablet is coated by blue film coating materials and a coated instant tablet is a special-shaped table with the disintegration time of 3'56' and the effervescent time of 6'16'. The effervescent tablet prepared by the method does not need taking with water and can be quickly dissolved when coming into contact with sputum in the mouth and partial medicines can be absorbed by blood through the transference of the mouth cavity, the sublingual gland and the tunica mucosa linguae. The effervescent tablet has the advantages of quick effect action, high medical absorption and biological availability, convenient oral application and little stimulation on the esophagus and the gastrointestinal tract.

Description

A kind of cerebral thrombosis effervescent tablet for the treatment of apoplexy and preparation method thereof

Invention field

The present invention relates to a kind of Chinese medicine preparation for the treatment of apoplexy, particularly be meant with the cerebral thrombosis dry extract to be cerebral thrombosis effervescent tablet of raw material and preparation method thereof.

Background technology

Apoplexy mainly betides middle-aged and elderly people, and 90% apoplexy occurs in the patient more than 40 years old, and big more sickness rate of age is then high more, and common every increase morbidity in 10 years old chance all can increase by 1 times.China is one of country that the apoplexy incidence rate is the highest in the world, existing apoplexy patient more than 2,000 ten thousand people.Acute cerebrovascular disease has leapt to urban population cause of the death first place, also increases by 1,500,000 new patients every year.Along with the arrival of China's aged tendency of population and shifting to an earlier date of apoplectic seizure age bracket, the sickness rate of apoplexy will be more and more higher.It has refractory more, the relapse rate height, take medicine all the life, complication is many, case fatality rate is high characteristics, be the formidable enemy of harm humans health always.Therefore, strengthen the prevention of old cerebrovascular and treat imperative.

The Western medicine that is used for the treatment of apoplexy at present is mainly thrombolytic medicine and channel blocker, but being it, the defective of using the thrombolytic drug therapy maximum only is applicable to that apoplexy takes place in back 3-6 hour, as above 3 hours, can cause intracerebral hemorrhage and cerebral edema behind the thrombolytic, increase the weight of the state of an illness on the contrary, these secondary effects are owing to reperfusion injury causes.Regrettably, generally can not arrive hospital in 3 hours after apoplexy takes place the patient, in addition, apoplexy also needs CAT or MRI to get rid of hemorrhagic apoplexy.Also need more than 30 minutes thus.Therefore, the probability that can accept thromboembolism treatment has only 1～2%.Chang Yong thrombolytic medicine has further limited acute paralytic's early treatment thus for how also can increase the hemorrhage probability in treatment back for treatments such as general enzyme, urokinase, streptokinase, defibrase, tissue-type plasminogen activator (tPA), r-ProUK in the market.Though some antithrombotic, as aspirin, warfarin, clopidogrel (clopidogrel) etc. is widely used in prophylactic treatment, and clearly, one can be used for the early stage Therapeutic Method of apoplexy safely and effectively is crucial often.

At present commercially available NAOXUESHUAN PIAN is by supporting in Song's Xu Shuwei " general Ji ability side " when the spherical pellet plus-minus, after be made into tablet and draft by the institute for drug control, Tianjin and be written into version " Drug Standard of Ministry of Public Health of the Peoples Republic of China Chinese traditional patent formulation preparation " the 19 in 1998, because of it is used for premonitory apoplexy and the convalescent curative effect of cerebral thrombosis is better, selected for use for the clinicist by income country " essential drug list ", and exploitation has oral liquid on the basis of tablet.

Because of Cornu Saigae Tataricae is put into " Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) ", belong to animals under first-class state protection; Calculus Bovis belongs to medicine source class medical material in short supply, is difficult to satisfy the clinical application needs, a large amount of dependence on import.Therefore, the inventor substitutes Cornu Saigae Tataricae, is used as medicine with the alternative Calculus Bovis of In vitro cultured Calculus Bovis, Calculus Bovis cultivating or artificial Calculus Bovis with Cornu Naemorhedi or Cornu Bubali through concentrating on studies, and former prescription technology is optimized and improved.In addition, though because former dosage form has effects such as antithrombotic, blood vessel dilating, because 4 flavors such as Radix Paeoniae Rubra are with crude drug fine powder direct compression, so exist bioavailability low, shortcoming such as the incomplete and dosage of drug absorption is big.The inventor carries out the dosage form process modification on the basis of traditional NAOXUESHUAN PIAN, the preparation of cerebral thrombosis effervescent tablet is concentrated on studies.As raw material, by Orthogonal Experiment and Design, filter out the preparation technology of optimization with the meticulous cerebral thrombosis dry extract that extracts of last stage, be developed to effervescent tablet, make it have taking convenience, mouthfeel is better, increases the advantage of patient's compliance, thereby is more suitable for clinical practice.Finished the present invention thus.

Summary of the invention

The object of the present invention is to provide that a kind of what treat apoplexy is the cerebral thrombosis effervescent tablet of raw material with the cerebral thrombosis dry extract;

Another object of the present invention has provided a kind of preparation method for the treatment of the cerebral thrombosis effervescent tablet of apoplexy.

The present invention realizes by following technical proposal;

Prescription (raw material consists of):

Flos Carthami 900g Radix Angelicae Sinensis 900g Hirudo (system) 900g Radix Paeoniae Rubra 900g

Semen Persicae 900g Rhizoma Chuanxiong 900g Radix Salviae Miltiorrhizae 900g Eupolyphaga Seu Steleophaga 225g

Cornu Naemorhedi or Cornu Bubali 600～1500g In vitro cultured Calculus Bovis, Calculus Bovis cultivating or artificial Calculus Bovis 3～30g

Method for making: prepare by the following step:

(1) pulverize above-mentioned raw material of Chinese medicine standby;

(2) get 5 flavors such as Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae, add entry, soak, vapor extraction volatile oil gets oil-water mixture; Filtrate and filtering residue are standby;

(3) in step (2), collect oil-water mixture in add the inclusion agents enclose, inclusion agents can be used beta-schardinger dextrin-, disperses enclose to get inclusion essential oil liquid, preferably adopts high shear process to comprise;

(4) water that step (2) is obtained is carried mother solution decompression low temperature and is reclaimed, and the concentrated liquid measure after the recovery equals the dosage of 5 flavor raw material of Chinese medicine such as Radix Angelicae Sinensis, adds ethanol and is stirred to that to contain the alcohol amount be 55～85% (V/V), and cold preservation is placed, filter, water extract-alcohol precipitation liquid;

(5) get Flos Carthami adding decocting and boil 1～3 time, filter, filtrate is recycled to the dosage of Flos Carthami;

(6) Flos Carthami filtering residue that obtains in the step (5) and the filtering residue in the step (2) merge, add 50～90% ethanol (V/V) that are equivalent to 6～10 times of amounts of this crude drug amount, heating and refluxing extraction 1～3 time, filter, water extract-alcohol precipitation liquid in alcohol extract and the step (4) merges, and reclaims ethanol to dosage and obtains alcohol extract;

(7) the water intaking trematodiasis adds soak with ethanol, and percolation is collected percolate, and it is standby that medicinal residues are flung to ethanol;

(8) percolate reclaims ethanol 1～3 times to dosage, adds the inclusion agents enclose and obtains Hirudo enclose liquid;

(9) get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge, add decocting and boil 1～3 friendship, filter, filtrate is recycled to dosage;

(10) get Cornu Naemorhedi or Cornu Bubali adding decocting and boil 3 times, filter filtrate for later use;

(11) Cornu Naemorhedi or Cornu Bubali medicinal residues add 0.25% sodium hydroxide solution (W/V), heating hydrolysis filters, and alkaline hydrolyzate adds rare HCl and transfers pH to 7.0, the Cornu Naemorhedi or the Cornu Bubali water extract that obtain with step (10) merge, and are recycled to dosage and obtain Cornu Naemorhedi or Cornu Bubali extracting solution;

(12) get In vitro cultured Calculus Bovis, Calculus Bovis cultivating or artificial Calculus Bovis and add entry, high shear disperses to pulverize;

(13) the inclusion essential oil aqueous solution that obtains of combining step (3), the saffron aqueous solution that step (5) obtains, the Radix Angelicae Sinensis that step (6) obtains, 6 flavor medicine alcohol extracts such as Flos Carthami, the Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution that step (8) obtains, Eupolyphaga Seu Steleophaga that step (9) obtains and Hirudo water extract, Cornu Naemorhedi that step (11) obtains or Cornu Bubali extracting solution, and the Calculus Bovis that step (12) obtains is pulverized liquid, add an amount of flavoring orange essence again, drying (can adopt spray drying, also can adopt vacuum drying, infrared drying, other suitable drying meanss such as microwave drying carry out drying), get the dry extract powder;

(14) method and the adjuvant of the routine of use preparation effervescent tablet are made effervescent tablet with the dry extract that step (13) obtains.Wherein, In vitro cultured Calculus Bovis is produced by Wuhan Dapeng Pharmaceutical Industry Co., Ltd, and Calculus Bovis cultivating is produced by China Bezoar Tech Development Co., and the artificial Calculus Bovis is produced by bass Europe, Shanghai pharmaceutcal corporation, Ltd.(down together)

The more excellent technical scheme of the present invention is:

Prescription:

Flos Carthami 900g Radix Angelicae Sinensis 900g Hirudo (system) 900g Radix Paeoniae Rubra 900g

Semen Persicae 900g Rhizoma Chuanxiong 900g Radix Salviae Miltiorrhizae 900g Eupolyphaga Seu Steleophaga 225g

Cornu Naemorhedi or Cornu Bubali 900～1200g In vitro cultured Calculus Bovis or Calculus Bovis cultivating 6～18g

Method for making: prepare by the following step:

(1) 4 flavors such as Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae were ground into the powder of 10～50 mesh sieves, and peach kernel powder was broken into the powder of 10～30 mesh sieves, and 3 flavors such as Flos Carthami, Hirudo, Eupolyphaga Seu Steleophaga were pulverized 20 orders, the Cornu Naemorhedi coarse pulverization, and In vitro cultured Calculus Bovis pulverizes, and is standby;

(2) get 5 flavors such as Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae, add the water that is equivalent to 6～10 times of this crude drug amounts, soaked 0～2 hour, vapor extraction volatile oil 3～9 hours filters, and gets oil-water mixture; Water carries mother solution and filtering residue keeps in Dark Place, and is standby;

(3) in step (2), collect oil-water mixture in add the beta-cyclodextrin inclusion compound agent of 70g～120g, 40～60 ℃ of water-baths adopt high shear process to disperse enclose 2～4 hours, shear rate is 1000～13000rpm, volatile oil beta-cyclodextrin inclusion compound liquid;

(4) water that step (2) is obtained is carried mother solution decompression low temperature and is recycled to dosage, adds ethanol and is stirred to and contains the alcohol amount and be 60-70% (V/V), and cold preservation is placed, filter, water extract-alcohol precipitation liquid, keep in Dark Place;

(5) get Flos Carthami and add 20～30 times of water that are equivalent to Flos Carthami crude drug amount, decoct 1～3 time, each 1～3 hour, filter, filtrate is recycled to dosage, keeps in Dark Place;

(6) the Flos Carthami filtering residue of step (5) and the filtering residue of step (2) are merged, 60～80% ethanol (V/V) (32.4～54L) that add 6～10 times of amounts of crude drug amount, heating and refluxing extraction 1～3 time, each 1～3 hour, filter, the water extract-alcohol precipitation liquid that this alcohol extract and step (4) obtain merges, and reclaims ethanol to dosage (5.4L), obtains alcohol extract;

(7) water intaking trematodiasis 70% ethanol (V/V) that adds 1 times of crude drug amount (0.9L) soaked 24 hours, percolation, and the collection percolate, medicinal residues are flung to ethanol, and are standby;

(8) percolate reclaims ethanol 2 times to dosage, adds the agent of 300～450g beta-cyclodextrin inclusion compound, and 60 ℃ of stirring in water bath, mixing speed are that 1000rpm gets Hirudo beta-cyclodextrin inclusion compound liquid, keep in Dark Place;

(9) get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge, add 3～5 times of the crude drug amounts that are equivalent to (3.375～5.625L) decocting boils 1～3 time, each 1～2 hour, filter, filtrate is recycled to dosage, preserves;

(10) get Cornu Naemorhedi or Cornu Bubali and add the decocting be equivalent to 10 times of crude drug amounts (promptly adding 10 times of amounts that the water yield is equivalent to Cornu Naemorhedi or Cornu Bubali dosage) and boil 3 times, each 2 hours, filter filtrate for later use;

(11) Cornu Naemorhedi or Cornu Bubali medicinal residues add 0.25% sodium hydroxide solution (W/V) that is equivalent to 8 times of crude drug amounts, and heating hydrolysis 4 hours filters, alkaline hydrolyzate adds rare HCL and transfers pH to 7.0, merge with Cornu Naemorhedi or Cornu Bubali water extract, be recycled to dosage, keep in Dark Place;

(12) get In vitro cultured Calculus Bovis or Calculus Bovis cultivating, add the water of 30 times of amounts, high shear disperses to pulverize 2 hours, and speed is 22000～28000rpm;

(13) the volatile oil beta-cyclodextrin inclusion compound aqueous solution that obtains of combining step (3); the saffron aqueous solution that step (5) obtains; step (6) must be extremely Radix Angelicae Sinensis; Radix Paeoniae Rubra; Rhizoma Chuanxiong; Radix Salviae Miltiorrhizae; Semen Persicae; 6 flavor medicine alcohol extracts such as Flos Carthami; the Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution that step (8) obtains; the water extract that step (9) obtains; Cornu Naemorhedi that step (11) obtains or Cornu Bubali extracting solution; pulverize liquid with the Calculus Bovis that step (12) obtains; add an amount of flavoring orange essence again; spray drying (also can adopt vacuum drying; infrared drying; other suitable drying meanss such as microwave drying carry out drying); inlet temperature: 160 ℃; outlet temperature: 82 ℃, get the dry extract powder.

(14) press dry extract 30～42%, crospolyvinylpyrrolidone 10～30%, mannitol 3～15%, anhydrous citric acid 6～12%, sodium bicarbonate 10～18%, microcrystalline Cellulose 5～16%, micropowder silica gel 2～3.5%, magnesium stearate 0.4%, Pulvis Talci 1.8%, the ratio mixing of Aspartane 0.5%, direct compression.

Optimized technical scheme of the present invention is:

Prescription:

Flos Carthami 900g Radix Angelicae Sinensis 900g Hirudo (system) 900g Radix Paeoniae Rubra 900g

Semen Persicae 900g Rhizoma Chuanxiong 900g Radix Salviae Miltiorrhizae 900g Eupolyphaga Seu Steleophaga 225g

Cornu Naemorhedi 1125g In vitro cultured Calculus Bovis 12.5g

Method for making: prepare by the following step:

(1) 4 flavors such as Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae were ground into the fine powder of 50 mesh sieves, and peach kernel powder was broken into the fine powder of 30 mesh sieves, and 3 flavors such as Flos Carthami, Hirudo, Eupolyphaga Seu Steleophaga were pulverized 20 orders, the Cornu Naemorhedi coarse pulverization, and In vitro cultured Calculus Bovis pulverizes, and is standby;

(2) get 5 flavors such as Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae, add the water that is equivalent to 10 times of crude drug amounts, soaked 1 hour, vapor extraction volatile oil filters, and gets oil-water mixture; Water carries mother solution and filtering residue keeps in Dark Place, and is standby;

(3) in step (2), collect oil-water mixture in add the beta-schardinger dextrin-of 93.3g, 50 ℃ of water-bath high shears disperseed enclose 4 hours, shear rate is 13000rpm, volatile oil beta-cyclodextrin inclusion compound liquid;

(4) water that step (2) is obtained is carried mother solution decompression low temperature and is recycled to dosage, adds 95% ethanol (V/V) and is stirred to that to contain the alcohol amount be 60% (V/V), and cold preservation was placed 24 hours, filter, water extract-alcohol precipitation liquid keeps in Dark Place;

(5) get Flos Carthami and add 30 times of water that are equivalent to the crude drug amount, decoct 2 times, each 1 hour, filter, filtrate is recycled to dosage and obtains saffron aqueous solution, keeps in Dark Place;

(6) the Flos Carthami filtering residue of step (5) and the filtering residue of step (2) are merged, 70% ethanol (V/V) that adds 10 times of amounts of crude drug amount, heating and refluxing extraction 3 times, each 3 hours, filter, the water extract-alcohol precipitation liquid that this alcohol extract and step (4) obtain merges, and reclaims ethanol to dosage, obtains alcohol extract 5.4L;

(7) water intaking trematodiasis 70% ethanol (V/V) that adds 1 times of crude drug amount soaked 24 hours, percolation, and percolation speed is 2.5ml/ minute, collects the percolate that is equivalent to 10 times of crude drug amounts, medicinal residues are flung to ethanol, and are standby;

(8) percolate reclaims ethanol 2 times to dosage, adds the 302.8g beta-schardinger dextrin-, 60 ℃ of stirring in water bath 1.5 hours, and mixing speed is that 1000rpm gets Hirudo beta-cyclodextrin inclusion compound liquid, keeps in Dark Place;

(9) get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge, add the decocting that is equivalent to 4 times of crude drug amounts and boil 3 times, each 2 hours, filter, filtrate is recycled to dosage, preserves;

(10) get Cornu Naemorhedi and add the decocting be equivalent to 10 times of doses and boil 3 times, each 2 hours, filter filtrate for later use;

(11) the Cornu Naemorhedi medicinal residues that step (10) obtained add 0.25% sodium hydroxide solution (W/V) that is equivalent to 8 times of crude drug amounts, heating hydrolysis 4 hours, keep the skin wet, filter, alkaline hydrolyzate adds rare HCL and transfers pH to 7.0, merge with the Cornu Naemorhedi water extract of step (10), be recycled to dosage and obtain the Cornu Naemorhedi extracting solution, keep in Dark Place;

(12) get In vitro cultured Calculus Bovis, add the water of 30 times of amounts, high shear disperses to pulverize 2 hours, and speed is 22000rpm;

(13) 6 flavor medicine alcohol extracts such as the volatile oil beta-cyclodextrin inclusion compound aqueous solution that obtains of combining step (3), saffron aqueous solution that step (5) obtains, Radix Angelicae Sinensis that step (6) obtains, Flos Carthami, Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution that step (8) obtains, water extract that step (9) obtains, Cornu Naemorhedi extracting solution that step (11) obtains,, the Calculus Bovis pulverizing liquid that obtains with step (12); add an amount of flavoring orange essence again; spray drying; inlet temperature: 160 ℃; outlet temperature: 82 ℃; rotating speed: 21000rpm (350HZ) gets the dry extract powder.

(14) press dry extract 39%, crospolyvinylpyrrolidone 15%, mannitol 3.5%, anhydrous citric acid 9.94%, sodium bicarbonate 15.06%, microcrystalline Cellulose 11.8%, micropowder silica gel 3%, magnesium stearate 0.4%, Pulvis Talci 1.8%, the ratio mixing of Aspartane 0.5%, direct compression is a coating material with OPADRY II (85G60989 BLUE), prepare into 18% water coating solution, inlet temperature: 50 ℃, the sheet bed tempertaure: 42 ℃, atomizing pressure: 3bar, coating pan rotating speed: 6～8rpm, coating weightening finish 3%, bag blue membrane clothing, promptly.

The cerebral thrombosis effervescent tablet that the present invention prepares has rapid-action, advantages such as bioavailability is high, taking convenience, constant product quality, good patient compliance.

The pressure limit of the plain sheet of effervescent tablet of the present invention is: 5.5N～12.5N, disintegration time can reach 2 ' 12 ", the effervescent time can reach 3 ' 50 ".Plain sheet blue membrane clothing material coating, instant is special-shaped tablets behind the coating, disintegration time reaches 3 ' 56 ", the effervescent time reaches 6 ' 16 ".The effervescent tablet that said method is made does not need water delivery service, runs into saliva and dissolve rapidly in mouth, and the part medicine can pass through oral cavity, Sublingual and periglottis transhipment, is absorbed into blood, and onset is rapid, has improved the absorption and the bioavailability of medicine.Play taking convenience and minimizing simultaneously to esophagus and gastrointestinal stimulation.

The prepared cerebral thrombosis effervescent tablet of the present invention can be used for the treatment of the blood stagnant in cerebral venation syndrome disease of apoplexy apoplex involving the channels and collaterals, the cerebral thrombosis convalescent period that comprises premonitory apoplexy and doctor trained in Western medicine, can effectively treat symptoms such as the caused hemiplegia of syndrome of blood stasis blocking brain, facial hemiparalysis, speech be unfavorable.

Description of drawings:

Accompanying drawing 1: Calculus Bovis grain size analysis figure

The specific embodiment:

Further specify the present invention below by embodiment.It should be understood that embodiments of the invention are to be used to illustrate the present invention rather than limitation of the present invention.Essence according to the present invention all belongs to the scope of protection of present invention to the simple modifications that the present invention carries out.In addition, in the present invention, (v/v) expression volume by volume concentration or volume ratio; (w/w) expression weight ratio concentration or weight ratio; (w/v) expression weight/volume specific concentration or by weight/volume, its corresponding unit is (grams per milliliter); (rpm) expression per minute revolution (rev/min); (h) expression hour.

Embodiment 1. Radix Angelicae Sinensis, Rhizoma Chuanxiong etc. extract the research of volatile oil technology

Radix Angelicae Sinensis, Rhizoma Chuanxiong, Semen Persicae three flavors are rich in volatile oil, fry in shallow oil as closing with other several flavors, may extract influentially to it, and compound Chinese medicinal preparation is emphasized synergism, so Radix Angelicae Sinensis, Rhizoma Chuanxiong, Semen Persicae three medicines of distinguishing the flavor of are closed to fry in shallow oil and carry oil, close to fry in shallow oil with five kinds of Chinese medicine and carry oil and compare.Both are leading indicator with the volatile oil yield, and the active component of each medicine of distinguishing the flavor of is that auxiliary characteristics is carried out orthogonal design, and the optimised process of gained compares to determine to put forward oily scheme.

1.1 closing to fry in shallow oil, three flavor medicines carry oily orthonormal design of experiments

Adopt orthogonal experiment method to determine the volatile oil optimum extraction condition of Radix Angelicae Sinensis, Rhizoma Chuanxiong, Semen Persicae three flavor medicines, select medical material fineness, amount of water, 3 factors of decocting time as the investigation factor, each factor is selected 3 level design L9 (3 ³) investigate, as following table:

Table 1 three flavor medicines close carries volatile oil factor level table

Level	Factor
				Extraction time A (hour)	Amount of water B (doubly)	Fineness C (order)
	1 2 3	3 6 9	6 8 10				40 10 20

Take by weighing Radix Angelicae Sinensis, Rhizoma Chuanxiong, each 70g of Semen Persicae according to the prescription ratio, 210g claims 9 parts altogether, experimentizes analysis result such as table 2 according to orthogonal experiment scheme table:

Table 2 three flavor medicines close fries in shallow oil the positive quadraturing design test analysis result

Standards of grading: get maximum level value X in each index components _MBe 100 minutes, each is tested this component content score and is X _i=X _i/ X _M* 100%, multiply by again that the total points of each index addition is this experiment score behind the coefficient separately.With volatile oil is leading indicator, ferulic acid, ligustrazine are auxiliary characteristics, we adopt method of weighting scores comprehensive assessment optimum condition, wherein the volatile oil yield accounts for 50% (coefficient is 0.5), ferulic acid (deriving from Rhizoma Chuanxiong, Radix Angelicae Sinensis) accounts for 30% (coefficient is 0.3), and ligustrazine accounts for 20% (coefficient is 0.2).

Statistical procedures such as following table:

Table 3 The results of analysis of variance

As from the foregoing, each factor influences size sequence to extraction and is: extraction time (A)＞amount of water (B)＞fineness (C), and A factor there was no significant difference, each factor optimum condition is: A ₂B ₃C ₁, promptly 40 orders add 10 times of amounts of water, extract 6 hours.

1.2 closing to fry in shallow oil, Radix Angelicae Sinensis, Rhizoma Chuanxiong, Semen Persicae, Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra five kinds of Chinese medicine carry oily orthonormal design of experiments

The best that adopts orthogonal experiment method to determine that Radix Angelicae Sinensis, Rhizoma Chuanxiong etc. 5 are distinguished the flavor of medicines is carried fritters of twisted dough spare, and adopting fineness, soak time, amount of water, extraction time is 4 factors, and 3 levels of each factor are investigated.The results are shown in Table 4:

Table 4 five kinds of Chinese medicine closes to fry in shallow oil and extracts volatile oil factor level table

Level	Factor
					Soak time A (hour)	Fineness B (order)	Amount of water C (doubly)	Extraction time D (hour)
	1	0	10	6					3
2					1	20	8	6
	3	2	50	10					9

Take by weighing Radix Angelicae Sinensis, Rhizoma Chuanxiong, Semen Persicae, Radix Salviae Miltiorrhizae, each 100g of Radix Paeoniae Rubra in the prescription ratio, 500g claims 9 parts altogether, experimentize according to orthogonal experiment scheme table 4, and extracted with diethyl ether 2 times of gained oil, a 25ml, a 10ml volatilizes ether, weighs.Analysis result such as table 5:

Table 5 five kinds of Chinese medicine closes fries in shallow oil the positive quadraturing design test analysis result

Numbering	A	B	C	D	Volatile oil content (%)	Ferulaic acid content (mg)	Ligustrazine content (mg)	Content of Danshensu (mg)	Protocatechualdehyde content (mg)	Paeoniflorin content (mg)	Score
												1	1	1	1	1	0.17	99.75	13.20	361.7	37.18	14.10	46.59
2	1	2	2	2	0.29	92.75	16.77	482.9	73.29	12.90	59.76
												3	1	3	3	3	0.58	116.6	20.27	513.1	82.60	12.80	84.99
4	2	1	2	3	0.30	64.00	24.37	545.7	120.34	13.41	64.51

Methods of marking closes to fry in shallow oil with three flavor medicines carries oil.Adopt method of weighting scores comprehensive assessment optimum condition, wherein the volatile oil yield is that leading indicator accounts for 40% (coefficient is 0.4), and ferulic acid accounts for 20% (coefficient is 0.2), and ligustrazine, protocatechualdehyde, danshensu, peoniflorin respectively account for 10% (coefficient is 0.1 separately).

Statistical procedures such as following table:

Table 6 The results of analysis of variance

As from the foregoing, each factor is closed five kinds of Chinese medicine and carried oil and influence size and be: extraction time (D)＞amount of water (C)＞fineness (B)＞soak time (A), wherein, B, C, D have utmost point significance to influence, and optimum condition is: A ₂B ₃C ₃D ₃, promptly 50 orders soaked 1 hour, added 10 times of water gagings, extracted 9 hours.

1.3 confirmatory experiment

Carry oil twice with optimum condition with the same amount medical material, the gained result carries out three flavor medicines and five kinds of Chinese medicine and closes and propose contrast.

Crude drug source: 1. Minxian County, Gansu Radix Angelicae Sinensis, Guanxian county, Sichuan Rhizoma Chuanxiong, Luoyang, Henan Semen Persicae, Pingyi, Shandong Radix Salviae Miltiorrhizae and Fushun Radix Paeoniae Rubra (with the five kinds of Chinese medicine quadrature)

2. Minxian County, Gansu Radix Angelicae Sinensis, Guanxian county, Sichuan Rhizoma Chuanxiong, Luoyang, Henan Semen Persicae, Luoyang, Henan Radix Salviae Miltiorrhizae and Wen County, Gansu Radix Paeoniae Rubra

The checking of table 7 Different Extraction Method

	Three flavor medicines	Five kinds of Chinese medicine
				Medicine source 1	Medicine source 2
		The volatile oil yield, (g) ferulaic acid content, (mg) ligustrazine content, (mg) content of Danshensu, (mg) protocatechualdehyde content, (mg) paeoniflorin content, (mg)	0.9531 88.65 57.0			1.5575 109.1 39.52 501.5 90.55 1613.75	1.2999 97.6 35.825 844.0 159.0 539.25

Two kinds of schemes are all with volatile oil yield (coefficient is 0.5), ferulaic acid content (0.3), and ligustrazine content (0.2) counts the score.See the following form:

Two kinds of schemes of table 8 get submeter

Three flavor medicines		Five kinds of Chinese medicine
				Medicine source 1	Medicine source 2
		Volatile oil ferulic acid ligustrazine score	47.12 44.26 89.61 54.76			77.01 54.47 90.21 72.89	64.26 48.73 81.77 63.10

From on can find out that three flavor medicines close to fry in shallow oil and carry oily comprehensive grading less than the five kinds of Chinese medicine comprehensive grading, illustrate distinguishes the flavor of Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra and Radix Angelicae Sinensis etc. three medicines are same boils the stripping that helps volatile oil extraction and ferulic acid, ligustrazine.

Embodiment 2. inclusion essential oil optimised process screening studies

The volatile oil that hybrid medicine proposes has stronger penetrating odor, if directly be used as medicine, is unfavorable for directly taking, and volatilization easily, be not easy to preservation, thereby be difficult to control the quality of preparation.As molecule behind enclose, dissolubility increases, stability improves, but the oil powdered that evaporates can prevent its volatilization, covers bad smell or taste simultaneously, reduces zest and toxic and side effects.Adopting β-CD (beta-schardinger dextrin-, down together) is the enclose material, and traditional medicine volatile oil is had better enclose effect.Its inclusion method and condition have been carried out following investigation.

2.1 inclusion method screening

The inclusion essential oil method has following several: 1. electric operated thermostatic paddling process; 2. magnetic force constant temperature paddling process; 3. ultrasonic method; 4. high shear dispersion method; 5. polishing.Wherein preceding four kinds is saturated water solution method, and back one is the solid-liquid method.And the used instrument of high shear dispersion method is a high shear dispersion boxshear apparatus.Under the same terms 5 kinds of methods have been done contrast now, used volatile oil system mixes the medical material steam distillation and proposes, and through the absolute ether extraction, the anhydrous sodium sulfate dehydration drying is yellow oil.

21.1 the preparation of the clathrate of different inclusion methods

Method is 1.～4.: take by weighing β-CD 5g, and adding distil water 75ml, heating for dissolving drips 0.5g volatile oil, 60 ℃ of constant temperature stirs, ultrasonic or high shear disperseed 2.5 hours, and cold preservation was placed 24 hours, and sucking filtration is used 15ml water, 5ml petroleum ether precipitation, 70 ℃ of drying under reduced pressure get faint yellow solid content.

Method is 5.: take by weighing β-CD 5g, put in the mortar, add water 20ml, add 0.5g oil, ground 2.5 hours, cold preservation was placed 24 hours, and sucking filtration use 15ml water, 5ml petroleum ether precipitation, and 70 ℃ of drying under reduced pressure must faint yellow solid content.

2.1.2 the yield of different inclusion methods

Above-mentioned clathrate is put round-bottomed flask, and adding distil water 100ml added thermal distillation 5 hours, collected volatile oil, and with extracted with diethyl ether, the anhydrous sodium sulfate dehydration drying volatilizes ether, and precision is weighed.Oil yield sees the following form:

The comparison of table 9 β-CDBao He volatile oil distinct methods

	Electronic paddling process	The magnetic agitation method	Ultrasonic method	Shearing method	Polishing
						Get oily number (g) oil yield %	0.1743 33.58	0.2268 45.27	0.169 33.81	0.2651 52.20	0.2147 42.68

As from the foregoing, five kinds of method oil yield comparative results are: shearing method＞magnetic agitation method＞polishing＞ultrasonic method＞electronic paddling process.So preferred high shear process that adopts of decision.

2.2 inclusion essential oil condition research

Selecting miscella and β-CD ratio, shear temperature, shear rate, shear time is 4 factors, and each factor is selected three levels, carries out the orthogonal experiment of four factors, three levels and observes, and sees the following form:

Table 10 volatile oil, β-CDBao He quadrature factor level table

Level	Factor
					Oil β-CD A	Enclose temperature B (℃)	Shear rate C (rpm)	The enclose time D (hour)
	1 2 3	1∶6 1∶8 1∶10	40 50 60	1000 1300 1600					2 3 4

Take by weighing β-CD, add 12.5 times of amount distilled water, heated and stirred makes dissolving, drip volatile oil 1g at constant temperature, control temperature and shear rate are carried out enclose to volatile oil, and keep certain hour, take out, cold preservation 24 hours, sucking filtration, with a small amount of petroleum ether precipitation, filtering residue was in 40 ℃ of drying under reduced pressure 4 hours, get the Powdered clathrate of yellow-white, weigh.

Get the 1g mixture of volatile oil, put round-bottomed flask, adding distil water 200ml added thermal distillation 5 hours, collected volatile oil, and with extracted with diethyl ether, the anhydrous sodium sulfate dehydration drying volatilizes ether, and precision is weighed.As a result, oilyly blank: 0.4053g; Then the response rate is: 40.53%.

Clathrate according to orthogonal table 10 gained is measured volatile oil by blank determination of recovery rates method.Gained result such as following table:

Table 11 shearing method β-CDBao He volatile oil orthogonal experiment analysis result

Annotate: solid yield=[clathrate weight/(β-CD+ volatile oil addition)] * 100%

Oil utilization rate=[gained volatilization oil mass/(the blank response rate of volatile oil addition *)] * 100%

Statistical procedures such as following table:

Table 12 The results of analysis of variance

As from the foregoing, each factor affecting size sequence of shearing method enclose volatile oil is: B (enclose temperature)＞D (enclose time)＞C (shear rate)＞A (β-CD, oil ratio example), and B, D factor have utmost point significant difference, and each factor optimum condition is: A ₂B ₂C ₂D ₃, promptly β-CD, oil ratio example are 1: 8,50 ℃ of 1300rpm sheared 4 hours.

2.3 inclusion essential oil process certification experiment

Carry out replication experiment by best clathrate process, n=2, the result is as follows:

The best preparation technology's replication experiment of table 13 volatile oil clathrate compound result

The experiment number	Volatile oil addition (g)	Filtrate solid yield (%)	Gained volatilization oil mass (g)	Oil utilization rate (%)
					1 2	2.0059 23.34	78.27 77.92	0.6741 0.7712	82.92 81.53

See by last table, oily utilization rate not high for the filtrate solid yield low due to, so enclose liquid is directly carried oil without the solid content that filters the evaporate to dryness gained, the calculating oil yield.

The research of water extract impurity removal process such as embodiment 3. Radix Angelicae Sinensis

Select for use precipitate with ethanol to handle or handle with natural clarifying agent, two kinds of remove impurity modes are carried out many indexs contrasts with mixing medical material water extract stock solution, thereby choose remove impurity mode preferably.

3.1 precipitate with ethanol impurity removal process

During with the remove impurity of ethanol precipitate with ethanol, Rhizoma Chuanxiong, Radix Angelicae Sinensis water extract are that 60% ethanol (V/V) precipitate with ethanol is good with concentration, and Radix Paeoniae Rubra, Radix Salviae Miltiorrhizae are then better with 60～70% (V/V) ethanol precipitate with ethanol, and then preferably using 60% (V/V) is the ethanol alcohol precipitation concentration.

Get Rhizoma Chuanxiong, Radix Angelicae Sinensis, Radix Paeoniae Rubra, Radix Salviae Miltiorrhizae, the Semen Persicae five tastes and carry the water extract behind the oil, 60 ℃ of lucifuge vacuum concentration to density are 1.02, add 95% ethanol (V/V) precipitate with ethanol, make it concentration and reach 60% (V/V), and cold preservation was placed 24 hours, sucking filtration, filtrate for later use.

3.2 clarifier impurity removal process

Investigated two kinds of clarifiers: Han Weisite clarifier (Tianjin Han Weisite scientific and technological development company limited) becomes clarifier (Tianjin became the clarification technique company limited in positive day) with positive sky.Get and mix medical material water extract, be concentrated into crude drug: water=1: 5, record its pH=5, be divided into two fens, stir the B component (every 100ml concentrated solution adds 30ml 1%B) that adds 3 parts of two kinds of clarifiers for 65 ℃, stirred 1 time in per 30 minutes respectively at constant temperature, add 1.5 parts of A after 2 hours, the same B of addition, centrifugal after 2 hours, it is standby to get supernatant.

3.3 precipitate with ethanol sample, clarifier sample and three kinds of sample liquid solid yields of water extract stock solution effective ingredient

Get the sample liquid that is equivalent to the 10g crude drug and be evaporated to driedly in 80 ℃ of water-baths, be taken out to 60 ℃ of evacuation 12 hours, taking-up is weighed, and calculates solid yield.The results are shown in following table:

The different impurity-removing methods of table 14 relatively

		Ferulic acid	Protocatechualdehyde	Danshensu	Peoniflorin	Ligustrazine	Solid yield (%)
								The water extract-alcohol precipitation sample	Content (mg) in the prescription	109.45	126.05	860.5	539		18.850
Account for the percent (%) of stock solution	85.78	89.81	84.28	74.19		46.00
							Han Weisite clarifier sample		Content (mg) in the prescription	121.35	136.5	921.0	536.5		40.494
Account for the percent (%) of stock solution	95.10	97.26	90.21	73.85		98.81
								Became the clarifier sample in positive day	Content (mg) in the prescription	125.85	128.8	917.5	521.0		39.673
Account for the percent (%) of stock solution	98.63	88.01	89.86	71.71		96.81

Water extract stock solution

127.60

140.35

1021

726.5

40.981

This shows that aqueous extraction-alcohol precipitation technology can keep the effective ingredient in the water extract stock solution more than 80%, and solid content has only 46% of stock solution, has improved the concentration of effective ingredient greatly, and reduced the extractum amount largely.Can keep more than 90% though add clarifier technology, solid content is 97% of a stock solution, does not play effective impurity-eliminating effect.Therefore, we preferably use the impurity removal process of aqueous extraction-alcohol precipitation technology as this side.

The research of embodiment 4. Flos Carthami extraction process by water

The safflower effective ingredients Carthamus yellow has better anticoagulation and significant to the treatment cerebral infarction to blood, and other water soluble ingredients also have certain effect to the corresponding symptom that apoplexy causes in the Flos Carthami simultaneously.Therefore, the Flos Carthami extraction process by water is that main total trap is estimated in order to flavochrome among the we, adopts orthogonal design that the factor that influences the aqueous soluble active constituent extraction is investigated.

4.1 Flos Carthami water is proposed the orthogonal experiment design

Selecting medical material fineness, solvent amount, extraction time, extraction time is 4 factors, and each factor is selected three levels, carries out the orthogonal experiment of four factors, three levels and investigates, and sees the following form:

Table 15 Flos Carthami water is carried quadrature factor level table

Level	Factor
					Medical material fineness A (order)	Solvent amount B (doubly)	Extraction time C (hour)	Extraction time D (inferior)
	1 2 3	0 20 50	20 25 30	1 2 3					1 2 3

Get flos carthami 5g (fineness is by last table) and press last table reflux, extract, in a certain amount of boiling water, sucking filtration, filtrate dilution certain multiple is measured trap according to spectrophotography at 401nm wavelength place.Orthogonal experiment results such as following table:

Table 16 Flos Carthami water is carried the orthogonal experiment analysis result

	Medical material fineness A (order)	Solvent amount B (doubly)	Extraction time C (hour)	Extraction time D (inferior)	Absorption value	Extension rate
							1 2 3 4 5 6 7 8 9 K 1 K 2 K 3	1 1 1 2 2 2 3 3 3 1.082 1.216 1.228	1 2 3 1 2 3 1 2 3 1.082 1.189 1.255	1 2 3 2 3 1 3 1 2 1.238 1.171 1.117	1 2 3 3 1 2 2 3 1 1.129 1.197 1.200	0.335 0.371 0.376 0.381 0.375 0.460 0.366 0.443 0.419	250 100 55.6 83.3 200 83.3 12.5 66.7 166.7

R

0.048

0.057

0.041

0.024

Statistical procedures is as follows:

Table 17 The results of analysis of variance

The result shows, factor B is the extraction solvent amount has appreciable impact to the extraction ratio of aqueous soluble active constituents such as Carthamus yellow, and all the other factors all do not make significant difference, and the optimum condition that experiment draws is A ₃B ₃C ₁D ₃Be that the medical material fineness is 50 orders, 30 times of water extract each 1 hour 3 times.Comprehensively to the consideration of energy-conservation process conditions such as easy, can be with A ₂B ₃C ₁D ₂Be considered as optimised process, promptly the medical material fineness is 20 orders, and 30 times of water extract each 1 hour 2 times.

4.2 confirmatory experiment

Ten times with the orthogonal experiment amount is the confirmatory experiment dosage, and the optimum condition 1 that experiment can be drawn contrasts with the optimised process of making by oneself 2, and experimental procedure is by 4.1.The result is as follows:

Table 18 Flos Carthami water is carried confirmatory experiment

Numbering	Condition	Absorption value
			1 2	(A 3B 3C 1D 3) (A 2B 3C 1D 2)	0.462 0.470

As seen from the above table, condition 1 is little with condition 2 difference, so select process conditions 2 (A for use ₃B ₃C ₁D ₃) be the Flos Carthami extraction process by water, promptly the medical material fineness is 20 orders, 30 times of water extract each 1 hour 2 times.

Embodiment 5 mixes the research of medical material alcohol extraction process

Five tastes medical materials such as Semen Persicae are after water is carried oil, and medicinal residues and Flos Carthami water are proposed the medicinal residues merging and carried out alcohol extraction, are intended to extract fat-soluble effective ingredient, and this is significant for comprehensive treatment cerebral embolism.Chinese medicine compound is emphasized synergism, considers that therefore this Six-element medical material merges alcohol extraction.The liposoluble constituent polarity of considering Six-element medical materials such as Semen Persicae is variant, drafts determining alcohol as the maximum variable factor, earlier alcohol extraction concentration is carried out single factor screening, to determine the alcohol extraction concentration range, again other factors is done the quadrature screening subsequently.

5.1 the single factor screening experiment of alcohol extraction concentration:

Get Six-element crude drug 24g (every flavor 4g), carry oil by 1,2,3,4 following steps of embodiment respectively and water is carried, medicinal residues add 95 ℃ of water-bath reflux, extract, of 10 times of Different concentrations of alcohol one hour, and centrifugal, supernatant is standby.

Design alcohol extraction concentration has: 30%, 50%, 70%, 90% (V/V).

5.1.1 determining of weight coefficient:

Because five kinds of Chinese medicine such as Radix Angelicae Sinensis has proposed part effective ingredient (ferulic acid, ligustrazine, peoniflorin) when water is carried oil, after the corresponding active constituent content of water being carried oil (five kinds of Chinese medicine) and the alcohol extraction relatively, with the weight coefficient of definite each index.

The results are shown in following table:

Table 19 different concentration ethanol is extracted the result

	Paeoniflorin content (mg)	Tanshinone I LA content (mg)	Ferulaic acid content (mg)	Ligustrazine content (mg)
					30% pure sample 50% pure sample 70% pure sample 90% pure sample	4.920 3.960 3.816 3.768	0.2210 2.4456 5.5440 4.7616	1.2240 0.7728 0.8880 0.6960	0.1864 0.1196 0.1088 0.0988

Water is carried oil phase and is answered composition and alcohol extraction active constituent content relatively:

Table 20 water is proposed the contrast with the effective ingredient of alcohol extraction

	Water extract extraction ratio (%)	Alcohol extract extraction ratio (%)
			Ferulic acid ligustrazine peoniflorin	0.1091 0.0358 0.5392	0.02418 0.00466 0.123

This shows that above-mentioned three kinds of active constituent contents have only water to carry 20% of fluid in the alcohol extract, so alcohol extraction is based on tanshinone.So each index components coefficient is decided to be: tanshinone is 0.55, and peoniflorin, ferulic acid, ligustrazine respectively are 0.15.

5.1.2 screening experiment result

Get maximum level X in each index components _mCount 100 fens, all the other sample sizes of this composition are kept the score and are X _i=(X _i/ X _m) * 100%, multiply by each tie element coefficient again after, the total points of each index addition is the score of this composition.Result such as following table:

Table 21 different concentration ethanol is extracted the score result

	Peoniflorin (0.15)	Tanshinone I LA (0.55)	Ferulic acid 0.15	Ligustrazine 0.15	Total points
						30% pure sample 50% pure sample 70% pure sample 90% pure sample	100 80.49 77.56 76.59	3.99 44.11 100 85.89	100 78.92 90.69 71.08	100 64.22 58.43 52.90	47.190 57.805 89.002 77.325

This shows that 70% pure sample mark is the highest, so be the alcohol extraction centre concentration.

5.2 Six-element medicine alcohol extraction orthogonal experiment design

Intend investigating ferulic acid, ligustrazine in the Rhizoma Chuanxiong, ferulic acid in the Radix Angelicae Sinensis, peoniflorin in the Radix Paeoniae Rubra, tanshinone isoreactivity component content is measured with HPLC in the Radix Salviae Miltiorrhizae.Select determining alcohol, solvent amount, extraction time and 4 factors of extraction time as the investigation factor, each factor is selected 3 levels to carry out orthogonal experiment to investigate, as following table:

Table 22 alcohol extraction orthogonal experiment factor level table

Level	Factor
					Determining alcohol A (%)	Solvent amount B (doubly)	Extraction time C (hour)	Extraction time D (inferior)
	1 2 3	60％ 70％ 80％	6 8 10	1 2 3					1 2 3

Get the used medicinal residues of single factor screening experiment and carry out reflux, extract, by last table, medicinal liquid is centrifugal, and supernatant is standby.Orthogonal experiment results is as follows:

Table 23 orthogonal experiment analysis result

Scoring system: with single factor screening experiment scoring system.

Statistical procedures:

Table 24 The results of analysis of variance

This shows that optimum condition is: A ₂B ₃C ₃D ₃, promptly concentration is that 10 times of amounts of 70% ethanol (V/V) are extracted each 3 hours 3 times.Because B, C, three factors of D all have appreciable impact to the index components extraction ratio, so can not only consider energy-conservation factor, confirmatory experiment is carried out in decision choosing then this condition.

5.4 optimum condition confirmatory experiment:

Get the used medicinal residues of orthogonal experiment, press A ₂B ₃C ₃D ₃Condition is extracted, and medicinal liquid is centrifugal, and supernatant is standby.The result is as follows:

Table 25 alcohol extraction confirmatory experiment result

Peoniflorin		TANSHINONES		Ferulic acid		Ligustrazine		Total points 95.17
									Content (mg) 7.0992	x i’ 98.90	Content (mg) 7.4232	x i’ 100	Content (mg) 1.620	x i’ 97.04	Content (mg) 0.396	x i’ 71.86

Confirmatory experiment shows, and this condition is the alcohol extraction optimum condition, and promptly concentration is that 10 times of amounts of ethanol (V/V) of 70% are extracted each 3 hours 3 times.

Embodiment 6. Hirudos, Eupolyphaga Seu Steleophaga Study on extraction

Hirudo is used ethanol percolation; Medicinal residues are carried with Eupolyphaga Seu Steleophaga merging water after flinging to ethanol.It is that index is made orthogonal experiment with nitrogen content that water is proposed experiment.

6.1 Hirudo alcohol extraction experiment:

It is best that the Hirudo alcohol extraction adopts 70% ethanol (V/V) that Hirudo is carried out the percolation effect, and collect 10 times of amount percolates, can reach the better extract rate, by above-mentioned condition Hirudo carried out alcohol extraction.

Water intaking trematodiasis 600g is ground into coarse powder, carries out percolation with 70% ethanol (V/V) for extracting solvent, and room temperature is 25 ± 3 ℃; Dip time is 24 hours; Speed is 2ml/ minute, collects percolate 6000ml, and is standby.It is standby that medicinal residues are flung to ethanol.

After percolate reclaimed ethanol, in 80 ℃ of evaporates to dryness of water-bath, 60 ℃ of vacuum dryings 24 hours were weighed, and the rate of extract is 8.41% (W/W).

6.2 Hirudo, the research of Eupolyphaga Seu Steleophaga extraction process by water

With the total nitrogen content of extract is index, and selective solvent soak time, extraction quantity of solvent, extraction time, 4 factors of extraction time are as the investigation factor, and each factor selects 3 levels to investigate its influence to extraction ratio, thereby determines best extraction process by water.

Get the Hirudo medicinal residues and the Eupolyphaga Seu Steleophaga coarse powder 15g that are equivalent to crude drug 60g, carry out water by table and propose experiment:

Table 26 Hirudo, Eupolyphaga Seu Steleophaga water are carried the factor level table

Level	Factor
					Soak time A (hour)	Solvent amount B (doubly)	Extraction time C (hour)	Extraction time D (inferior)
	1 2 3	0 1 2	3 4 5	1.0 1.5 2.0					1 2 3

The total nitrogen content of extract is measured and press medical material nitrogen content assay method mensuration, gained experimental data and the results are shown in following table:

Table 27 Hirudo, Eupolyphaga Seu Steleophaga water are carried the orthogonal experiment analysis result

Statistical procedures:

Table 28 The results of analysis of variance

The result shows that extraction process by water adds 4 times of water not soak, and extracts 3 times, and each 1.5 hours for well.

Make confirmatory experiment with optimum condition, n=4, the result is as follows, nitrogen content average: 2859.95mg; Extraction ratio average: 3.813%.This shows, available above-mentioned as Hirudo and Eupolyphaga Seu Steleophaga extraction process by water condition.Be extraction process by water not soak, add 4 times of water, extract 3 times, each 1.5 hours for well.

Embodiment 7. Hirudo ethanol extract clathrate process

Hirudo extract has strong stench flavor, and taste is not good, is difficult to directly take.Be used as medicine as direct evaporate to dryness and make us and to accept, be unfavorable for taking of effervescent tablet.Intend ethanol extract being carried out enclose, be intended to cover bad smell, increase its water solublity with β-CD or You Teqi aqueous dispersion (acrylic resin, R  HM company).With a certain amount of β-CD or You Teqi aqueous dispersion enclose ethanol extract, make and play flavored action, avoid heavy again because of using a large amount of adjuvants to increase sheet as far as possible.

By than the sense of taste behind the enclose accessory package Heshui trematodiasis ethanol extract of sedan-chair variety classes and consumption thereof, determine the technology of enclose.Concrete steps are as follows:

7.1 β-CDBao He

Measure the alcohol extract 300ml that is equivalent to crude drug 30g, add β-CD saturated solution (2 times, 3 times, 4 times of ethanol extract) respectively and stirred 1.5 hours for 60 ℃ in water-bath with 1000rpm, take out in 70 ℃ of heating of water-bath evaporate to dryness with electric blender.

7.2 You Teqi enclose

Get alcohol extract 300ml, measure the You Teqi (dosage 10%, 15%, 20%) of certain volume respectively, and 10% triethyl citrate of You Teqi, with electric blender with 1000rpm (rev/min) stirred 1.5 hours in 60 ℃ of water-baths, take out in 70 ℃ of heating of water-bath evaporate to dryness.

7.3 two kinds of enclose relatively

By gustation relatively, result such as following table:

The different inclusion method contrasts of table 29 Hirudo ethanol extract

	β-CDBao He			The You Teqi enclose
							Institute adds adjuvant amount (g) sense of taste	2 times 5.046 is not good	3 times 7.569 abnormal flavour is arranged slightly	Be as good as sense of taste for 4 times 10.092	10% 3 are not good	15% 4.5 are not good	20% 6 have abnormal flavour

Take all factors into consideration, because Hirudo ethanol extract the rate of extract is little, its added adjuvant amount is also little, and the dosage behind the preparations shaping increases few, therefore preferably gets the still receptible 4 times of β-CDBao He schemes of sense of taste.

The technical study of the mode of being used as medicine of embodiment 8. Cornu Naemorhedi

There is Cornu Saigae Tataricae simply in the former prescription.Cornu Saigae Tataricae belongs to protection species in imminent danger, is put in the species scope of " Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) ".Because of than, sustainable development has positive effect to Endangered species to seek suitable succedaneum, also reduces simultaneously the prescription cost greatly.Cornu Naemorhedi proves that after deliberation its chemical constituent and pharmacological action and Cornu Saigae Tataricae are basic identical, and consumption is 8～10 times.Therefore now use for Cornu Saigae Tataricae with 10 times of amount Cornu Naemorhedi.Show that after deliberation it is more obvious than powder suspensoid refrigeration function to be used as medicine after the Cornu Saigae Tataricae hydrolysis.So intend planting to the quality of cerebral thrombosis treatment effect directly into the pharmaceutical worker with pharmacodynamics The effects Cornu Naemorhedi hydrolysis process and powder.

8.1 Cornu Naemorhedi hydrolysis process

Basic hydrolysis is handled: get Cornu Naemorhedi 562.5g, coarse pulverization adds 10 times of water gagings and decocts 3 times, and each 2 hours, to filter, filtrate is placed standby.Filtering residue adds 8 times of amount 0.25% sodium hydroxide solutions (W/V), and heating hydrolysis 4 hours constantly keeps the skin wet, cooled and filtered, and filtrate merges decocting liquid and hydrolyzed solution with dilute hydrochloric acid adjust pH to 7.0, and heating is concentrated into and contains crude drug amount 10%, and is standby.

8.2 Cornu Naemorhedi disintegrating process

Get Cornu Naemorhedi 562.5g, coarse pulverization, in micronizing place micronizing, degree of grinding is as follows:

Table 30 degree of grinding relatively

Volume average particle size (μ m)	Surface area mean diameter (μ m)	d(0.5)(μm)	d(0.9)(μm)
				37.96	22.07	30.14	78.13

8.3 the preparation of two kinds of technology samples

8.3.1 Cornu Naemorhedi hydrolysis process sample

Except that Cornu Naemorhedi all the other flavour of a drug carry by preceding definite technology branch in a recipe quantity (being Radix Angelicae Sinensis 900g) do not get, remove impurity and enclose.Get half amount and merge with Cornu Naemorhedi hydrolyzation sample extract, spray drying gets powdered extract powder 984.5g altogether, add to be ground to thin In vitro cultured Calculus Bovis powder 6.25g, and mixing, as Cornu Naemorhedi hydrolysis process sample, standby.

8.3.2 Cornu Naemorhedi powder technology sample

Get second half all the other flavour of a drug extracts, spray drying, powdered extract powder 834g altogether, add 562.5g and cultivate 6.25g with being ground to outside the thin bovine calculus powder opisthosoma through the Cornu Naemorhedi powder of micronizing, mixing, as Cornu Naemorhedi powder technology sample, standby.

8.4 two kinds of technology samples to the drug action of acute cerebral ischemia animal model relatively

8.4.1 influence to intraluminal middle cerebral artery occlusion in rats blocking-up model nervous symptoms and infarct size

Rat model to the moulding cerebral ischemia of this experiment employing line bolt method formation office inserts intracranial to anterior cerebral artery with nylon wire from the total arteries and veins of neck, the blood flow of blocking-up middle cerebral artery, thus form middle cerebral artery blocking-up (MCAO) model.After rat is clear-headed, a side quadriplegia and rotation asymmetry occur, use the Longa point system, 1,3,6,12,24 hour neurological handicap symptom is marked; With infarction size behind the TTC dyeing demonstration cerebral ischemia 24h.Laboratory animal SD rat, body weight 250-300g, result such as following table:

The influence of table 31 pair cerebral ischemia (intraluminal middle cerebral artery occlusion in rats thromboembolism MCAO) cerebral infarction volume (X ± s)

Grouping	Dosage	Number of animals	Cerebral infarction volume (%)
				Sham operated rats model group hydrolysis process group powder technology group	330mg/kg 460mg/kg	10 10 10 10	0 24.96±8.24 13.87±6.14 ** 15.73±6.46 *

^*Compare p＜0.05 with model group ^*Compare p＜0.01 with model group

The influence of table 32 pair cerebral ischemia (intraluminal middle cerebral artery occlusion in rats thromboembolism MCAO) nervous symptoms (X ± s)

Grouping	Dosage	Animal	Nervous symptoms scoring (Longa55 divides system)
									(mg/kg)	(n)	1h	3h	6h	12h	24h
Sham operated rats model group hydrolysis process group powder technology group	330 460	10 10 10 10	0 2.22±0.97 1.33±0.71 *1.45±0.69 *	0 2.63±0.92 2.22±0.97 2.73±0.47	0 3.17±0.98 1.63±0.92 * 1.90±0.32 *	0 2.63±0.92 1.17±0.98 * 1.80±0.41 *	0 3.17±0.98 0.62±0.17 ** 0.81±0.21 **

^*Compare p＜0.05 with model group ^*Compare p＜0.01 with model group

8.42 influence to rat bilateral ligation model brain water content and cerebral index

The SD male rat (250～300g), with 20% urethane i.p anesthesia, cut skin of neck, separate the bilateral neck always affectionately and ligation, after three hours, broken end is got brain, claims full cutaneous horn, dry weight, calculates rat brain water content and cerebral index, as following table:

The influence of table 33 pair cerebral ischemia (rat bilateral ligation) cerebral edema and cerebral index (X ± s)

Grouping	Dosage	Number of animals	Brain water content (%)	Cerebral index
					Normal group model group hydrolysis process group powder technology group	330mg/kg 460mg/kg	10 10 9 9	76.25±1.38 78.67±0.99 * 78.09±1.26 *# 78.19±2.21	0.59±0.002 0.62±0.03 * 0.54±0.03## 0.55±0.03#

Annotate: heavy * 100/ body weight brain water content (%)=(weight in wet base-dry weight)/weight in wet base * 100% of cerebral index=cutaneous horn

^*Compare P＜0.05 with normal group; # and model group compare p＜0.05 ## and model group compares p＜0.01

8.43 to thrombotest

Rat artery-vein bypass thrombotic model is adopted in this experiment, with 20% urethane anesthetized rat, cuts skin of neck, separates a side common carotid artery.Between artery and vein, insert the rapid ethylene tube that fills a 50U/ml heparin sodium aqua and the long 4# operation of the 5cm that weighs an in advance silk thread in, form the A-V short circuit.50U/kg.i.v behind the heparin sodium aqua, weigh after removing floating blood, deduct the silk thread original weight, be the weight in wet base of thrombosis, thrombosis was put into 60 ℃ of baking boxs dry 4 hours, weigh after the cooling, be the thrombosis dry weight.Calculate the thrombosis suppression ratio then, see the following form:

The thrombotic influence of the total artery-vein bypass of table 34 pair rat neck (X ± s)

Grouping	Dosage	Number of animals	Wet weight of thrombus	The thrombosis dry weight
					Normal control group aspirin group hydrolysis process group powder technology group	100 330mg/kg 460mg/kg	7 7 5 5	14.83±4.12 9.14±1.25 ** 12.32±4.80 * 13.18±3.52	3.09±1.15 2.07±0.34 * 2.65±0.31 * 2.88±0.35 *

^*Compare p＜0.05 with normal group ^*Compare p＜0.01 with normal group

Comprehensive drug experimental result, hydrolysis process group are than the good drug efficacy of powder technology group, and both have the significance difference, and the rate of extract of while hydrolysis process is planted little directly into the pharmaceutical worker than powder, reduced dosage.Therefore preferred Cornu Naemorhedi hydrolysis process is as the Cornu Naemorhedi extraction process.

The disintegrating process research of embodiment 9. In vitro cultured Calculus Boviss

The In vitro cultured Calculus Bovis consumption is less, and more valuable, can directly be used as medicine.Bovine calculus powder is broken into microgranule is used as medicine, can increase its dissolubility, help the quick stripping of preparation and improve bioavailability.Consider Calculus Bovis to be pulverized, pulverize the particle diameter of back in water, screen the process conditions of pulverizing by surveying it with high shear mark crushing technology.

Breaking method: get In vitro cultured Calculus Bovis and be ground to fine powder with mortar, take by weighing 2g, add water 60ml, put high shear by different condition and disperse boxshear apparatus to pulverize, mixed liquor is measured particle diameter with particle size analyzer.Result such as following table:

Table 35 In vitro cultured Calculus Bovis grain size analysis report

Rotating speed (rpm)

Time (h)

Volume average particle size (μ m)

Surface area mean diameter (μ m)

d(0.5)(μm)

d(0.9)(μm)

22000 28000

0.5 1.0 2.0 3.0 1.0

11.049 8.996 6.808 6.774 8.186

2.514 2.251 1.798 1.251 1.585

5.106 4.430 3.119 2.081 2.929

30.308 23.881 17.629 18.688 23.157

To rotating speed 22000rpm mapping, analysis time is to the influence of degree of grinding, the result as shown in Figure 1:

As from the foregoing; Rotating speed strengthens little to grain diameter influence.Time energy appreciable impact size, but time expand, influence little to powder particle diameter again after 2.0 hours.So from energy-conservation angle, but optimum condition: and rotating speed 22000rpm, the pulverizing time is 2.0 hours.

Medical research shows that the human body the intestines and stomach is about 15 μ m to particulate optimal absorption fineness, because the granule of micron Chinese medicine reaches optimal absorption fineness level, effective ingredient obviously increases at the gastrointestinal dissolubility, thereby increase bioavailability of medicament, accelerate the onset time (Chen Li of medicine, Wu Yiping. micron Chinese medicine and technology of preparing Chinese herbal medicine thereof, 2002,33 (10): 865～868).So the above-mentioned optimum condition of high shear process reaches this purpose.

Embodiment 10. spray dryinges

Merging water extract-alcohol precipitation liquid such as Radix Angelicae Sinensis (be the distinguish the flavor of water extract of medicinal material extract volatile oil of Radix Angelicae Sinensis etc. 5; adding alcohol after concentrating is 60% to containing the alcohol amount; cold preservation is placed; filter; obtaining filtrate promptly is); saffron aqueous solution; alcohol extracts such as Radix Angelicae Sinensis; water extracts such as Hirudo; the Cornu Naemorhedi extracting solution; the volatile oil beta-cyclodextrin inclusion compound aqueous solution; Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution; In vitro cultured Calculus Bovis is pulverized liquid; add an amount of flavoring orange essence; mix homogeneously; the sample solution that takes a morsel records mixed liquor solid content 0.0766g/g; density is 1.023g/ml, advances the spray drying tower drying.Inlet temperature: 160 ℃, outlet temperature: 82 ℃, obtain dry extract, mix homogeneously.Results of grain size analysis sees the following form:

The efficient cerebral thrombosis dry extract grain size analysis report of table 36

Volume average particle size (μ m)	Surface area mean diameter (μ m)	d(0.5)(μm)	d(0.9)(μm)
				25.996	6.814	8.830	72.760

By result in the table as can be known, the dry extract granularity is the ultra micro granularity, helps the preparation of preparations shaping technology, effervescent tablet and medicine in absorption by human body.

The preparation of embodiment 11. cerebral thrombosis effervescent tablets

In the prescription ratio material 161.86kg that gets it filled, wherein Radix Angelicae Sinensis, Rhizoma Chuanxiong, Flos Carthami, Radix Paeoniae Rubra, Radix Salviae Miltiorrhizae, Semen Persicae, each 19kg of Hirudo, Eupolyphaga Seu Steleophaga 4.8kg, Cornu Naemorhedi 23.8kg, In vitro cultured Calculus Bovis 262.6g gets the 8.32kg beta-schardinger dextrin-simultaneously.By determined extraction process each medical material is extracted, concrete grammar is with the laboratory amplification test.Radix Angelicae Sinensis, Rhizoma Chuanxiong are cut into small pieces, and Radix Paeoniae Rubra, Radix Salviae Miltiorrhizae, peach kernel powder are broken into fine powder, and 3 flavors such as Flos Carthami, Hirudo, Eupolyphaga Seu Steleophaga are ground into coarse powder, the Cornu Naemorhedi pregrounding, and In vitro cultured Calculus Bovis pulverizes, and is standby; Get 5 flavors such as Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae, mix homogeneously is divided into 5 parts, in the sand hill that the warp layer cloth of packing into is made, add the water (190L) that is equivalent to 10 times of crude drug amounts, soaked vapor extraction volatile oil 1 hour, filter, collect volatile oil, obtain the 33L oil water mixture; Filtrate and filtering residue keep in Dark Place, and be standby; With collect the volatile oil oil water mixture add the beta-cyclodextrin inclusion compound contain 1.96kg, 50 ℃ of water-bath high shears disperse to shear enclose 4 hours, shear rate is 13000rm ^-1, get volatile oil beta-cyclodextrin inclusion compound liquid; Water extracts such as Radix Angelicae Sinensis decompressions low temperature is recycled to dosage, adds 95% ethanol (V/V) and is stirred to that to contain the alcohol amount be 60% (V/V), and cold preservation was placed 24 hours, filter, water extract-alcohol precipitation liquid keeps in Dark Place; Get Flos Carthami and add the 30 times of water (570L) that are equivalent to the crude drug amount, decoct 2 times, each 1 hour, filter, filtrate is recycled to dosage, keeps in Dark Place; 70% ethanol (V/V) that filtering residues such as Flos Carthami filtering residue and Radix Angelicae Sinensis merge to add 10 times of amounts of crude drug amount (190L), heating and refluxing extraction 3 times, each 3 hours, filter, water extracts such as alcohol extract and Radix Angelicae Sinensis merge recovery ethanol to dosage; 70% ethanol (V/V) that the water intaking trematodiasis adds 1 times of crude drug amount (19L) soaked 24 hours, percolation, and percolation speed is 2.5ml/ minute, collects the percolate that is equivalent to 10 times of crude drug amounts, medicinal residues are flung to ethanol, and are standby; Percolate reclaims ethanol 2 times to dosage, adds the beta-schardinger dextrin-saturated aqueous solution that contains 6.36kg, 60 ℃ of stirring in water bath 1.5 hours, and mixing speed is that 1000rpm gets Hirudo beta-cyclodextrin inclusion compound liquid, keeps in Dark Place; Get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge to add the water (95.2L) that is equivalent to 4 times of crude drug amounts and decoct 3 times, each 2 hours, filter; Get Cornu Naemorhedi and add the water (238L) be equivalent to 10 times of crude drug amounts and decoct 3 times, each 2 hours, filter filtrate for later use; The Cornu Naemorhedi medicinal residues add be equivalent to 8 times of crude drug amounts 0.25% NaOH (W/V) (190.4L), heating hydrolysis 4 hours keeps the skin wet, and filters, alkaline hydrolyzate adds rare HCL and transfers pH to 7.0, keeps in Dark Place; Get In vitro cultured Calculus Bovis, be ground to fine powder, put the middle high shear of 30 times of water (7.9L) and pulverized 2 hours, speed is 22000rpm.Merge water extracts such as alcohol extracts such as water extract-alcohol precipitation liquid, saffron aqueous solution, Radix Angelicae Sinensis, Hirudo, Cornu Naemorhedi extracting solution, volatile oil beta-cyclodextrin inclusion compound aqueous solution, Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution, In vitro cultured Calculus Bovis pulverizing liquid such as Radix Angelicae Sinensis; mix homogeneously; the sample solution that takes a morsel records mixed liquor solid content 0.0766g/g; density is 1.023g/ml, advances the spray drying tower drying.Inlet temperature: 160 ℃, outlet temperature: 82 ℃, obtain dry extract, mix homogeneously.Discharging dry extract 43.11kg after the spray drying.The rate of extract is 25.33% (W/W), moisture content 1.66% (W/V).Press dry extract 39%, crospolyvinylpyrrolidone (XL) 15%, mannitol 3.5%, anhydrous citric acid 9.94%, sodium bicarbonate 15.06%, microcrystalline Cellulose (PH102) 11.8%, micropowder silica gel 3%, magnesium stearate 0.4%, Pulvis Talci 1.8%, the ratio mixing of Aspartane 0.5%, direct compression, (Shanghai Colorcon Coating Technology Co., Ltd produces with Opadry OPADRY II (85G60989 BLUE), be coating material down together), prepare into 18% water coating solution, inlet temperature: 50 ℃, sheet bed tempertaure: 42 ℃, atomizing pressure: 3bar, coating pan rotating speed: 6～8rpm, coating weightening finish 3%, bag blue membrane clothing, promptly.

The cerebral thrombosis effervescent tablet main pharmacodynamics experiment that embodiment 12. embodiment 11 make

Experimental drug and reagent:

The cerebral thrombosis effervescent tablet: Shanghai Institute of Pharmaceutical Industry provides, lot number: 040727; Be blue membrane sugar coated tablet (the 0.725g/ sheet contains 0.2828g dry extract/sheet, quite contains crude drug 1.06g, and promptly every gram contains crude drug 1.46g)

NIAOXUESHUAN GANJINGAOFEN: Shanghai Institute of Pharmaceutical Industry provides, lot number: 040702, and every gram contains crude drug 3.74g.

XIAOSHUAN TONGLUO PIAN 0.375g/ sheet: Jisheng Pharmaceutical Factory, Nanchang, Jiangxi (lot number: 040401) (positive control medicine)

NAOXUESHUAN PIAN 0.3g/ substrate: the Jilin Province willow is closed brightness and sends out Pharmacy stock Co., Ltd's (lot number: 20040601) (former dosage form contrast medicine)

The chloral hydrate analytical pure: going up seamount Pu Chinese workers has company's (lot number: 20040212)

Red tetrazolium (2,3,5 triphenyite trazolium, TTC) powder: Chinese Medicine Shanghai chemical reagents corporation (lot number: F20021123).

Heparin sodium injection 2ml/ props up, 12500u/ml: Tianjin Biochemical Pharmaceutical Factory (lot number: 20040507)

Sodium chloride analytical pure: Tianjin northization glass purchase and sale center (lot number: 20020605)

Ethylurethanm chemical pure: go up seamount Pu chemical industry company limited (lot number: 20040517)

5ml heparin sodium vacuum test tube, (lot number: 041020): Hubei gold Fructus Pruni development in science and technology company limited is produced

3ml specification sodium citrate vacuum test tube (lot number: 040915): produce by Hubei gold Fructus Pruni development in science and technology company limited.

Concrete experiment is as follows:

12.1 protective effect to focal cerebral ischemia in rats

Get 80 male SD rats, 250g-350g is divided into eight groups at random, that is: sham operated rats, the normal saline matched group, positive drug control group (XIAOSHUAN TONGLUO PIAN group 0.6gd-1kg-1), former dosage form group (NAOXUESHUAN PIAN group 0.5gd-1kg-1), dry extract group (the crude drug of cerebral thrombosis effervescent tablet, the not starch-containing additive component that waits) 0.3gd-1kg-1), cerebral thrombosis effervescent tablet small dose group (0.4gd-1kg-1), cerebral thrombosis converges rises dosage group (0.8gd-1kg-1) in the sheet, the heavy dose of group of cerebral thrombosis effervescent tablet (1.6gd-1kg-1), every group 10, each group is irritated gastric capacity and is about 5mlkg-1, every day 2 times, successive administration 14 days.The middle cerebral artery line bolt method (middle cerebral artery occlusion, MCAO) the preparation rat persistence focal cerebral ischemia model that adopt the Longa method and give improvement.With reference to 6 grades of point systems of Zea 3h after surgery, 6h, 12h and 24h mark: 0 minute: impassivity damage symptom; 1 minute: can not full extension offside fore paw; 2 minutes: turn-take laterally; 3 minutes; Topple over to offside; 4 minutes: can not spontaneously walk loss of consciousness; 5 minutes: death.

Postoperative 24h, rat is anaesthetized the back deeply with 10% chloral hydrate and puts to death, get brain rapidly, freezing 10min, get coronalplane from optic chiasma and evenly be cut into 5, the brain sheet of every thick about 2mm, put into 2% TTC solution (37 ℃) dyeing 30min, take out then and observe, as seen pink zone is a normal cerebral tissue, and white area is an infarcted region. after measuring brain infarction area, separate infarcted region and normal cerebral tissue, infarcted region and normal cerebral tissue are carried out weighing, obtain the cerebral infarction percent by volume according to infarction brain weight and full brain weight ratio.See the following form:

The influence of cerebral infarction volume due to the table 37 pair rat face kitchen range cerebral ischemia (X ± s)

Group	Number of animals	Dosage	Infarction volume/brain cumulative volume (%)
				Dosage group small dose group in the sham-operation group model group anti-cerebral-thrombosis tablet group eliminating thrombus and removing obstruction in channels group dry extract group high dose group	10 10 8 8 9 8 8 9	0.5g/d/kg 0.6g/d/kg 0.3g/d/kg 1.6g/d/kg 0.8g/d/kg 0.4g/d/kg	0 27.51±4.71 17.35±4.67 **18.80±6.94 **12.72±5.51 ***11.18±3.35 ***◆ 14.60±7.00 **15.37±7.21 **

^*P＜0.05, ^*P＜0.01, ^{* *}Compare with model group P＜0.001

◆ p＜0.05 and former preparation group;  P＜0.01 and positive controls

Annotate: 1. dose column is represented the every Kg body weight of rat, every day dosage.

2. test used Rapid Dose Calculation foundation: the animals administer amount of positive controls and former preparation group is all based on the clinical administration amount, and by the dose,equivalent commutation table on " new drug evaluation basis with put into practice ", the every day consumption of will being grown up is converted to rat consumption every day.The dry extract group is based on clinical plan dosage, and by the dose,equivalent commutation table on " new drug evaluation basis with put into practice ", the every day consumption of will being grown up is converted to rat consumption every day.Below each the table with.

The influence of nervous symptoms due to the table 38 pair focal cerebral ischemia in rats (X ± s)

Group	Number of animals	Dosage	Nervous symptoms (by system scoring in 5 fens)
						3 hours	6 hours	24 hours

Dosage group small dose group in the sham-operation group model group anti-cerebral-thrombosis tablet group eliminating thrombus and removing obstruction in channels group dry extract group high dose group

10 10 10 9 10 10 10 9

0.5g/d/kg 0.6g/d/kg 0.3g/d/kg 1.6g/d/kg 0.8g/d/kg 0.4g/d/kg

0 2.80±1.3 1.60±0.70 *1.67±1.12 *1.20±0.60 ***0.90±0.44 ***1.10±0.57 ***1.44±0.53 **

0 3.00±1.33 1.70±0.67 * 1.67±0.50 ** 1.20±0.63 *** 1.00±0.71 *** 1.30±0.48 ** 1.56±0.53 **

0 3.56±1.59 2.11±1.27 * 1.89±1.27 * 1.60±1.35 ** 1.30±0.53 *** 1.40±0.69 ** 1.67±0.87 **

^*P＜0.05, ^*P＜0.01, ^{* *}Compare with model group P＜0.001

From as can be seen last, the cerebral thrombosis effervescent tablet can alleviate the cerebral infarction volume due to the focal cerebral ischemia in rats and improve the nervous symptoms that is caused by cerebral ischemia according to dosage.Model group rat Infarction volume is 27.51% ± 4.71, and high dose group is 11.18% ± 3.35; Middle dosage group is 14.60% ± 7.00; Small dose group is 15.37% ± 7.21); Compare with model group, each group hundred rates that descend are respectively 59.36%, 46.93% and 44.13%; Compare p＜0.01 with model group and former preparation group; Difference all has extremely significantly meaning.

12.2 to the thrombotic influence of rat neck artery-vein bypass

Get 72 male SD rats, 2.50g-350g is divided into seven groups at random, except that the normal saline matched group is 12, and all the other every group 10.Each group is irritated the stomach amount and is 5mlkg-1, every day 2 times, successive administration 14 days.Adopt neck artery-vein by-pass method to form thrombosis.Connect the tube for transfusion that a section long, centres have been placed one section long No. 4 silk thread of 3～4cm with two sections short polyethylene tubes, and it is filled standby intubate with the heparin-saline of 50uml-1.20% urethane intraperitoneal injection of anesthesia rat (6mlkg-1), treat Animal Anesthesia after, dorsal position is fixed, position, neck center routine operation otch is isolated right carotid and left side external jugular vein.The polyethylene tube that has prepared is inserted at two ends respectively, promptly forms the bypass of rat neck artery-vein, and during No. 4 silk threads in tube for transfusion of blood flow, the matsurface that platelet etc. promptly are attachable to silk thread forms thrombosis.In the intubate process, need give the heparin-saline anticoagulant of the quiet notes of rat 0.1ml100g-1.Form the artery-vein bypass after 15 minutes, take out silk thread, weighing after removing floating blood on the filter paper promptly obtains wet weight of thrombus.The back thrombosis of will weighing is put in the drying baker, takes out after under 60 ℃ dry 4 hours and weighs, and is the thrombosis dry weight.The results are shown in following table:

The table 39 pair thrombotic influence of rat neck artery-vein bypass (X ± s)

Group	Number of animals	Dosage	Thrombus weight (mg)
					Weight in wet base	Dry weight
			Dosage group small dose group in the physiological saline group anti-cerebral-thrombosis tablet group eliminating thrombus and removing obstruction in channels group dry extract group high dose group	12 8 8 8 10 10 8			0.5g/d/kg 0.6g/d/kg 0.3g/d/kg 1.6g/d/kg 0.8g/d/kg 0.4g/d/kg	24.23±8.21 17.10±3.89 * 14.93±4.50 ** 11.49±2.29 ** 11.08±3.10 ** 12.26±3.55 ** 15.56±5.13 *	4.93±2.17 3.83±1.09 3.45±0.87 1.89±0.67 ** 2.88±1.02 * 3.13±1.38 * 3.35±1.44

^*P＜0.05, ^*Compare with the normal saline group P＜0.01

The result shows, the cerebral thrombosis effervescent tablet has certain inhibitory action to rat neck artery-vein bypass thrombosis, 0.8g/d/kg and 1.6g/d/kg relatively is respectively 55% and 50% to the inhibition percentage rate and the normal saline group of wet weight of thrombus, and the inhibition percentage rate of thrombosis dry weight is respectively 54% and 37%; Its effect is more more obvious than former preparation and positive controls.

12.3 influence to platelet aggregation

63 of male SD rats, body weight 250-300 gram is divided into 7 groups at random by body weight.Normal control group oral (irritate and feed) normal saline, the administration group gives cerebral thrombosis effervescent tablet 0.4,0.8 and 1.6g/kg respectively, and positive control drug is irritated stomach and is given XIAOSHUAN TONGLUO PIAN 0.6g/kg.The administration volume is the 0.5ml/100g body weight.Behind the successive administration 14 days, 20% urethane intraperitoneal anesthesia rat (6mlkg-1) is got blood from common carotid artery.

With sodium citrate blood sampling tube 3ml, jolting immediately is in case hemostasis liquid solidifies.By the turbidimetry for Determination platelet aggregation, use the LBY-NJ2 platelet aggregation instrument, the anticoagulant whole blood is with the centrifugal 8min of 1000rmin-1 under the room temperature, and preparation platelet rich plasma (PRP) is placed to close in the 0.5mlEppendorf pipe to cover and is preserved, and puts in the constant temperature hole standby; Remaining blood is platelet poor plasma (PPP) through centrifugal 10 minutes gained supernatants of 3000rpm.Making it platelet count with PPP adjustment PRP is 40～500,000 mm-3, get 200 μ l PPP and put into square cup in order to zeroing, getting 200 μ l PRP adds in the square cup that fills stirrer unshakable in one's determination, after the PPP zeroing, extract the square cup of PPP, put into the square cup of PRP, platelet count to be shown adds final concentration and is 3.5 μ molL-1 ADP, 11 μ l and can obtain 1,3 and 5min platelet aggregation rate (PAG) and maximum platelet aggregation rate (MPAG) numerical value.Experimental data is expressed as standard deviation (x ± s), check the significance of administration group and normal control group mean difference with t.The results are shown in following table:

The influence of table 40 pair rat platelet aggregation (X ± s)

Group	Number of animals	Dosage	Platelet aggregation rate (t/min)			Maximum agglutination rate
							1	3	5
			Dosage group small dose group in the heavy dose of group of physiology salt group anti-cerebral-thrombosis tablet group eliminating thrombus and removing obstruction in channels group dry extract group	9 8 8 8 9 9 9	0 0.5g/d/kg 0.6g/d/kg 0.3g/d/kg 1.6g/d/kg 0.8g/d/kg 0.4g/d/kg					38.66±7.91 30.59±8.93 23.26±12.35 **22.13±9.02 **23.10±10.08 **27.33±12.37 *27.91±13.18	48.79±12.51 37.26±11.68 27.44±13.78 **26.06±10.36 **27.77±9.08 **37.80±16.86 39.00±14.3	41.36±19.07 22.49±13.73 *18.34±11.54 **17.79±8.89 **18.02±6.02 **29.66±14.54 33.73±14.34	50.46±12.17 40.48±10.17 31.33±12.5 **29.2±19.58 **30.84±9.84 **39.81±17.1 40.63±13.03

^*P＜0.05, ^*Compare with the normal saline group P＜0.01

As seen from the above, after cerebral thrombosis effervescent tablet 1.6g/d/kg continuous irrigation stomach gives 14 days, the inductive platelet aggregation of ADP there is inhibitory action, to 1,3,5 minutes and maximum the gathering wait the suppression ratio of each time point to be respectively 40.24%, 43.08%, 56.43% and 42.13%; (P＜0.01).Its effect obviously is better than former preparation NAOXUESHUAN PIAN group.

12.4 influence to rat blood viscosity

Get 70 male SD rats, 250g-350g is divided into 7 groups at random by body weight, 10 every group.Award dosage group (0.8gd-1kg-1) in normal saline (normal control and model control group), positive drug control group (NAOXUESHUAN PIAN group 0.5gd-1kg-1), former preparation group (XIAOSHUAN TONGLUO PIAN group 0.6gd-1kg-1), crude drug group (cerebral thrombosis effervescent tablet dry extract group 0.3gd-1kg-1), cerebral thrombosis effervescent tablet small dose group (0.4gd-1kg-1), the cerebral thrombosis effervescent tablet, the heavy dose of group of cerebral thrombosis effervescent tablet (1.6gd-1kg-1) respectively.Each group is irritated the stomach amount and is 5mlkg-1, every day 2 times, and successive administration 14 days, the 15th day rat got blood with 20% urethane intraperitoneal anesthesia rat (6mlkg-1) from common carotid artery.With heparin sodium blood sampling tube 3ml, jolting immediately prevents blood coagulation.Behind Sysmex kx-21 type cellanalyzer sample introduction, measure packed cell volume (HCG) and platelet count (PLD) value under the room temperature.

From whole blood, take out 0.5ml in addition and contain heparin whole blood adding Brookfield DV-III+RHEOMETER type rotating cylinder viscometer, under 37 ℃, measure respectively high shear rate (HS, 37.5/s), middle shear rate (MS, 150/s) and low shear rate (LS, 300/s) the whole blood viscosity value (WBV) under; Other gets 2ml and contained heparin whole blood 3000rmin-1 centrifugal 10 minutes, and draw upper plasma 0.5ml and add viscometer, the high cutting-out of mensuration plasma viscosity value (PV, 225/s).The result is as follows:

The influence of table 41 pair rat platelet counting and packed cell volume (X ± s)

Group	Number of animals	Dosage	Platelet count ( *10 9/1)	Packed cell volume (%)
					Dosage group small dose group in the heavy dose of group of physiological saline group anti-cerebral-thrombosis tablet group eliminating thrombus and removing obstruction in channels group dry extract group	9 8 8 8 9 9 9	0.5g/d/kg 0.6g/d/kg 0.3g/d/kg 1.6g/d/kg 0.8g/d/kg 0.4g/d/kg	980±298 876±230 767±63 744±306 * 752±167 * 857±98 937±214	40.2±72.5 43.9±4.74 45.9±4.82 47.92±4.81 45.9±15.39 43.61±4.23 43.13±4.21

^*P＜0.05, ^*Compare with the normal saline group P＜0.01

The influence of table 42 pair rat blood viscosity (shear rate 1/s) (X ± s)

Group	Number of animals	Dosage	WBV (whole blood)			PV (blood plasma) (225/s)
							LS(37.5/s)	MS(90/s)	HS(300/s)
			Dosage group small dose group in the heavy dose of group of physiological saline group anti-cerebral-thrombosis tablet group eliminating thrombus and removing obstruction in channels group dry extract group	10 10 8 8 9 9 9	0.5g/d/kg 0.6g/d/kg 0.3g/d/kg 1.6g/d/kg 0.8g/d/kg 0.4g/d/kg					13.99±4.13 11.49±2.23 11.10±1.22 10.70±1.17 * 9.75±1.60 * 8.95±2.48 ** 9.83±2.27 *	11.18±5.20 8.47±1.79 9.42±1.49 7.12±1.00 * 7.21±0.94 * 6.55±1.80 ** 7.42±1.72	6.40±1.52 5.17±0.46 * 5.04±0.79 * 5.01±0.57 * 5.13±0.53 * 4.29±0.40 ** 5.32±1.71	1.55±0.58 1.63±0.99 1.30±0.11 1.21±0.09 1.28±0.15 1.31±0.07 1.33±0.18

^*P＜0.05, ^*Compare with the normal saline group P＜0.01

Can draw by last two tables, cerebral thrombosis effervescent tablet 1.6g/d/kg and dry extract group (being the crude drug of this tablet) 0.3g/d/kg continuous irrigation stomach 14 days, influence to platelet count, compare with the normal saline group, 23.26% and 24.08% (p＜0.05) that descended does not respectively all have obvious influence to packed cell volume.The results are shown in Table 5.Continuous 14 days oral cerebral thrombosis effervescent tablets of rat to the viscosity of whole blood and normal saline matched group relatively, at shear rate 37.5/s, 90/s, 300/s, can obviously reduce whole blood viscosity, and the effect of 0.8g/d/kg group is more obvious, each dosage group is obvious influence to the rat plasma viscosity.

The cerebral thrombosis effervescent tablet long term toxicity test of embodiment 13. embodiment 11

Rat is carried out the long term toxicity test in 9 weeks with the cerebral thrombosis effervescent tablet of embodiment 11.Promptly with 21.2g, 10.6g, 5.3g crude drug/kg/ day in continuous 9 weeks of rat oral gavage administration of three dosage, the result show this medicine to rat outward appearance sign, behavioral activity, diet, feces, body weight, hematological indices, blood parameters, system become celestial, main organs coefficient and histopathologic examination all do not find any toxic reaction.After two weeks of drug withdrawal the part rat is carried out convalescent period and observe, repeat above-mentioned every inspection, also no abnormality seen.Above-mentioned dosage be equivalent to respectively to be grown up 100,50 and 25 times of clinical plan consumption show that it is safe that clinical plan adopts the dosage of 12.72g crude drug/60kg/ day.

In sum, use that multinomial new technique extracts and the cerebral thrombosis effervescent tablet focal cerebral ischemia in rats is had significant protective effect; Can alleviate the cerebral infarction volume due to the focal cerebral ischemia in rats and improve the nervous symptoms that causes by cerebral ischemia according to dosage; Rat carotid artery thrombosis there is certain inhibitory action; Can improve each index of hemorheology aspect preferably.With 21.2g, 10.6g, 5.3g crude drug/kg/ day in continuous 9 weeks of rat oral gavage administration of three dosage, do not see toxic reaction or death.

The cerebral thrombosis effervescent tablet acute toxicity test of embodiment 14. embodiment 11

Cerebral thrombosis effervescent tablet 63.6g crude drug/kg/ day with embodiment 11 is given mouse stomach, none death of mice in 7 days, and this dosage is equivalent to 300 times of adult's plan consumption (12.72g crude drug/60kg/ day).It is clinical safer to point out this medicine to be used for.

The preparation of embodiment 15. cerebral thrombosis effervescent tablets

In the prescription ratio material 8.0586Kg that gets it filled, wherein Flos Carthami, Radix Angelicae Sinensis, Hirudo, Radix Paeoniae Rubra, Semen Persicae, Rhizoma Chuanxiong, each 900g of Radix Salviae Miltiorrhizae, Eupolyphaga Seu Steleophaga 225g, Cornu Naemorhedi 1125g, In vitro cultured Calculus Bovis 12.5g, β-go back dextrin 396.1g.4 flavors such as Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae were ground into the fine powder of 50 mesh sieves, and peach kernel powder was broken into the fine powder of 30 mesh sieves, and 3 flavors such as Flos Carthami, Hirudo, Eupolyphaga Seu Steleophaga were pulverized 20 orders, the Cornu Naemorhedi coarse pulverization, and In vitro cultured Calculus Bovis pulverizes, and is standby; Get 5 flavors such as Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae, add the water that is equivalent to 10 times of crude drug amounts, soaked 1 hour, vapor extraction volatile oil filters, and gets oil-water mixture; Filtrate and filtering residue keep in Dark Place, and be standby; Collect oil-water mixture in add the β of 93.3g-go back dextrin, 50 ℃ of water-bath high-shear emulsifying enclose 4 hours, emulsifying rate is 13000rpm, volatile oil beta-go back dextrin enclose liquid; Water extracts such as Radix Angelicae Sinensis decompressions low temperature is recycled to dosage, adds 95% ethanol and is stirred to that to contain the alcohol amount be 60%, and cold preservation was placed 24 hours, filter, water extract-alcohol precipitation liquid keeps in Dark Place; Get Flos Carthami and add 30 times of water that are equivalent to the crude drug amount, decoct 2 times, each 1 hour, filter, filtrate is recycled to dosage, keeps in Dark Place; Filtering residues such as Flos Carthami filtering residue and Radix Angelicae Sinensis merge 70% ethanol that adds 10 times of amounts of crude drug amount, heating and refluxing extraction 3 times, and each 3 hours, filter, water extract-alcohol precipitation liquid such as alcohol extract and Radix Angelicae Sinensis merge recovery ethanol to dosage; The water intaking trematodiasis adds 70% soak with ethanol 24 hours of 1 times of crude drug amount, percolation, and percolation speed is 2.5ml/min, collects the percolate that is equivalent to 10 times of crude drug amounts, medicinal residues are flung to ethanol, and are standby; Percolate reclaims ethanol 2 times to dosage, adds 302.5g β-go back dextrin, and 60 ℃ of stirring in water bath 1.5 hours, mixing speed are that 1000rpm gets Hirudo β-go back dextrin enclose liquid, keep in Dark Place; Get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge to add the decocting that is equivalent to 4 times of crude drug amounts and boil 3 times, each 2 hours, filter, filtrate is recycled to dosage, preserves; Get Cornu Naemorhedi and add the decocting be equivalent to 10 times of crude drug amounts and boil 3 times, each 2 hours, filter filtrate for later use; The Cornu Naemorhedi medicinal residues add 0.25% sodium hydroxide solution that is equivalent to 8 times of crude drug amounts, and heating hydrolysis 4 hours keeps the skin wet, and filter, and alkaline hydrolyzate adds rare HCL and transfers pH to 7.0, merge with Cornu Naemorhedi water extract to be recycled to dosage, keep in Dark Place; Get the Calculus Bovis high shear and disperse to pulverize 2 hours, speed is 22000rm-1; Merge water extracts such as alcohol extracts such as water extract-alcohol precipitation liquid, saffron aqueous solution, Radix Angelicae Sinensis, Hirudo such as Radix Angelicae Sinensis, Cornu Naemorhedi extracting solution, volatile oil beta-go back dextrin enclose aqueous solution, Hirudo ethanol extract β-go back dextrin enclose aqueous solution and Calculus Bovis pulverizing liquid and an amount of flavoring orange essence; spray drying; inlet temperature: 160 ℃; outlet temperature: 82 ℃; rotating speed: 2100rpm (350HZ) gets the dry extract powder.Discharging dry extract 1.9815kg after the spray drying.The rate of extract is 24.59% (W/W).Press dry extract 39%, crospolyvinylpyrrolidone (XL) 17%, mannitol 3.5%, anhydrous citric acid 9%, sodium bicarbonate 16%, microcrystalline Cellulose (PH102) 11%, micropowder silica gel 2%, magnesium stearate 0.4%, Pulvis Talci 1.8%, Aspartane ratio mixing O.5%, direct compression is a coating material with OPADRY II (85G60989 BLUE), prepare into 18% water coating solution, inlet temperature: 50 ℃, the sheet bed tempertaure: 42 ℃, atomizing pressure: 3bar, coating pan rotating speed: 6-8rpm, coating weightening finish 3%, bag blue membrane clothing, promptly.

The preparation of embodiment 16. cerebral thrombosis effervescent tablets

In the prescription ratio material 451.59kg that gets it filled, wherein Radix Angelicae Sinensis, Rhizoma Chuanxiong, Flos Carthami, Radix Paeoniae Rubra, Radix Salviae Miltiorrhizae, Semen Persicae, each 57kg of Hirudo, Eupolyphaga Seu Steleophaga 14.4kg, Cornu Naemorhedi 38kg, In vitro cultured Calculus Bovis 190g gets the 6.25kg beta-schardinger dextrin-simultaneously.By determined extraction process each medical material is extracted, concrete grammar is with the laboratory amplification test.Radix Angelicae Sinensis, Rhizoma Chuanxiong are cut into small pieces, and Radix Paeoniae Rubra, Radix Salviae Miltiorrhizae, peach kernel powder are broken into fine powder, and 3 flavors such as Flos Carthami, Hirudo, Eupolyphaga Seu Steleophaga are ground into coarse powder, the Cornu Naemorhedi pregrounding, and In vitro cultured Calculus Bovis pulverizes, and is standby; Get 5 flavors such as Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae, mix homogeneously is divided into 5 parts, in the sand hill that the warp layer cloth of packing into is made, add the water (190L) that is equivalent to 10 times of crude drug amounts, soaked vapor extraction volatile oil 1 hour, filter, collect volatile oil, obtain the 70L oil water mixture; Filtrate and filtering residue keep in Dark Place, and be standby; With collect the volatile oil oil water mixture add the beta-cyclodextrin inclusion compound contain 4.2kg, 50 ℃ of water-bath high shears disperse to shear enclose 4 hours, shear rate is 13000rm ^-1, get volatile oil beta-cyclodextrin inclusion compound liquid; Water extracts such as Radix Angelicae Sinensis decompressions low temperature is recycled to dosage, adds 95% ethanol (V/V) and is stirred to that to contain the alcohol amount be 60% (V/V), and cold preservation was placed 24 hours, filter, water extract-alcohol precipitation liquid keeps in Dark Place; Get Flos Carthami and add the 30 times of water (570L) that are equivalent to the crude drug amount, decoct 2 times, each 2 hours, filter, filtrate is recycled to dosage, keeps in Dark Place; Filtering residues such as Flos Carthami filtering residue and Radix Angelicae Sinensis merge 70% ethanol (V/V) that adds 10 times of amounts of crude drug amount, heating and refluxing extraction 3 times, and each 3 hours, filter, water extracts such as alcohol extract and Radix Angelicae Sinensis merge recovery ethanol to dosage; 70% ethanol (V/V) that the water intaking trematodiasis adds 1 times of crude drug amount soaked 24 hours, percolation, and percolation speed is 2.5ml/ minute, collects the percolate that is equivalent to 10 times of crude drug amounts, medicinal residues are flung to ethanol, and are standby; Percolate reclaims ethanol 2 times to dosage, adds the beta-schardinger dextrin-saturated aqueous solution that contains 13.32kg, 60 ℃ of stirring in water bath 1.5 hours, and mixing speed is that 1000rpm gets Hirudo beta-cyclodextrin inclusion compound liquid, keeps in Dark Place; Get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge to add the water (95.2L) that is equivalent to 4 times of crude drug amounts and decoct 3 times, each 3 hours, filter; Get Cornu Naemorhedi and add the water (238L) be equivalent to 10 times of crude drug amounts and decoct 3 times, each 2 hours, filter filtrate for later use; The Cornu Naemorhedi medicinal residues add be equivalent to 8 times of crude drug amounts 0.25% NaOH (W/V) (190.4L), heating hydrolysis 4 hours keeps the skin wet, and filters, alkaline hydrolyzate adds rare HCL and transfers pH to 7.0, keeps in Dark Place; Get In vitro cultured Calculus Bovis, be ground to fine powder, put in 30 times of water high shear and pulverized 3 hours, speed is 22000rpm.Merge water extracts such as alcohol extracts such as water extract-alcohol precipitation liquid, saffron aqueous solution, Radix Angelicae Sinensis, Hirudo, Cornu Naemorhedi extracting solution, volatile oil beta-cyclodextrin inclusion compound aqueous solution, Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution, In vitro cultured Calculus Bovis pulverizing liquid such as Radix Angelicae Sinensis; mix homogeneously; the sample solution that takes a morsel records mixed liquor solid content 0.0783g/g; density is 1.031g/ml, advances the spray drying tower drying.Inlet temperature: 160 ℃, outlet temperature: 82 ℃, obtain dry extract, mix homogeneously.Discharging dry extract 109.38kg after the spray drying.The rate of extract is 23.89% (W/W), moisture content 1.87% (W/V).Press dry extract 30%, crospolyvinylpyrrolidone (XL) 30%, mannitol 4%, anhydrous citric acid 10%, sodium bicarbonate 16%, microcrystalline Cellulose (PH102) 5%, micropowder silica gel 2%, magnesium stearate 0.4%, Weihe stone powder 1.8%, Aspartane ratio mixing O.5%, direct compression is a coating material with OPADRY II (85G60989 BLUE), prepare into 18% water coating solution, inlet temperature: 50 ℃, the sheet bed tempertaure: 42 ℃, atomizing pressure: 3bar, coating pan rotating speed: 6～8rpm, coating weightening finish 3%, bag blue membrane clothing, promptly.

The preparation of embodiment 17. cerebral thrombosis effervescent tablets

In the prescription ratio material 323.72kg that gets it filled, wherein Radix Angelicae Sinensis, Rhizoma Chuanxiong, Flos Carthami, Radix Paeoniae Rubra, Radix Salviae Miltiorrhizae, Semen Persicae, each 38kg of Hirudo, Eupolyphaga Seu Steleophaga 9.6kg, Cornu Naemorhedi 57.6kg, In vitro cultured Calculus Bovis 525.2g gets the 16.64kg beta-schardinger dextrin-simultaneously.By determined extraction process each medical material is extracted, concrete grammar is with the laboratory amplification test.Radix Angelicae Sinensis, Rhizoma Chuanxiong are cut into small pieces, and Radix Paeoniae Rubra, Radix Salviae Miltiorrhizae, peach kernel powder are broken into fine powder, and 3 flavors such as Flos Carthami, Hirudo, Eupolyphaga Seu Steleophaga are ground into coarse powder, the Cornu Naemorhedi pregrounding, and In vitro cultured Calculus Bovis pulverizes, and is standby; Get 5 flavors such as Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae, mix homogeneously is divided into 5 parts, in the sand hill that the warp layer cloth of packing into is made, add the water that is equivalent to 10 times of crude drug amounts, soaked vapor extraction volatile oil 2 hours, filter, collect volatile oil, obtain the 68L oil water mixture; Filtrate and filtering residue keep in Dark Place, and be standby; With collect the volatile oil oil water mixture add the beta-cyclodextrin inclusion compound contain 4kg, 50 ℃ of water-bath high shears disperse to shear enclose 2 hours, shear rate is 13000rm ^-1, get volatile oil beta-cyclodextrin inclusion compound liquid; Water extracts such as Radix Angelicae Sinensis decompressions low temperature is recycled to dosage, adds 95% ethanol (V/V) and is stirred to that to contain the alcohol amount be 60% (V/V), and cold preservation was placed 24 hours, filter, water extract-alcohol precipitation liquid keeps in Dark Place; Get Flos Carthami and add 30 times of water that are equivalent to the crude drug amount, decoct 2 times, each 1 hour, filter, filtrate is recycled to dosage, keeps in Dark Place; 70% ethanol (V/V) that filtering residues such as Flos Carthami filtering residue and Radix Angelicae Sinensis merge to add 10 times of amounts of crude drug amount (190L), heating and refluxing extraction 3 times, each 3 hours, filter, water extracts such as alcohol extract and Radix Angelicae Sinensis merge recovery ethanol to dosage; 70% ethanol (V/V) that the water intaking trematodiasis adds 1 times of crude drug amount soaked 24 hours, percolation, and percolation speed is 2.5ml/ minute, collects the percolate that is equivalent to 10 times of crude drug amounts, medicinal residues are flung to ethanol, and are standby; Percolate reclaims ethanol 2 times to dosage, adds the full Heshui solution of the beta-schardinger dextrin-that contains 12.82kg, 60 ℃ of stirring in water bath 2 hours, and mixing speed is that 1000rpm gets Hirudo beta-cyclodextrin inclusion compound liquid, keeps in Dark Place; Get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge to add the decocting that is equivalent to 4 times of crude drug amounts and boil 3 times, each 1 hour, filter; Get Cornu Naemorhedi and add the decocting be equivalent to 10 times of crude drug amounts and boil 3 times, each 3 hours, filter filtrate for later use; The Cornu Naemorhedi medicinal residues add be equivalent to 8 times of crude drug amounts 0.25%NaOH (W/V) (190.4L), heating hydrolysis 4 hours keeps the skin wet, and filters, alkaline hydrolyzate adds rare HCL and transfers pH to 7.0, keeps in Dark Place; Get In vitro cultured Calculus Bovis, be ground to fine powder, put in 30 times of water high shear and pulverized 2 hours, speed is 22000rpm.Merge water extracts such as alcohol extracts such as water extract-alcohol precipitation liquid, saffron aqueous solution, Radix Angelicae Sinensis, Hirudo, Cornu Naemorhedi extracting solution, volatile oil beta-cyclodextrin inclusion compound aqueous solution, Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution, In vitro cultured Calculus Bovis pulverizing liquid such as Radix Angelicae Sinensis; mix homogeneously; the sample solution that takes a morsel records mixed liquor solid content 0.0738g/g; density is 1.012g/ml, advances the spray drying tower drying.Inlet temperature: 160 ℃, outlet temperature: 82 ℃, obtain dry extract, mix homogeneously.Discharging dry extract 87.23kg after the spray drying.The rate of extract is 25.63% (W/W), moisture content 1.46% (W/V).Press dry extract 39%, crospolyvinylpyrrolidone (XL) 10%, mannitol 15%, anhydrous citric acid 6%, sodium bicarbonate 15%, microcrystalline Cellulose (PH102) 8%, micropowder silica gel 3%, magnesium stearate 0.4%, Pulvis Talci 1.8%, the ratio mixing of Aspartane 0.5%, direct compression is a coating material with OPADRY II (85G60989 BLUE), prepare into 18% water coating solution, inlet temperature: 50 ℃, the sheet bed tempertaure: 42 ℃, atomizing pressure: 3bar, coating pan rotating speed: 6～8rpm, coating weightening finish 3%, bag blue membrane clothing, promptly.

The preparation of embodiment 18. cerebral thrombosis dry extracts

In the prescription ratio material 24.62kg that gets it filled, wherein Radix Angelicae Sinensis, Rhizoma Chuanxiong, Flos Carthami, Radix Paeoniae Rubra, Radix Salviae Miltiorrhizae, Semen Persicae, each 2.88kg of Hirudo, Eupolyphaga Seu Steleophaga 0.72kg, Cornu Naemorhedi 3.68kg, In vitro cultured Calculus Bovis 60g gets the 6.42kg beta-schardinger dextrin-simultaneously.By determined extraction process each medical material is extracted, concrete grammar is with the laboratory amplification test.Radix Angelicae Sinensis, Rhizoma Chuanxiong are cut into small pieces, and Radix Paeoniae Rubra, Radix Salviae Miltiorrhizae, peach kernel powder are broken into fine powder, and 3 flavors such as Flos Carthami, Hirudo, Eupolyphaga Seu Steleophaga are ground into coarse powder, the Cornu Naemorhedi pregrounding, and it is liquor-saturated, standby that In vitro cultured Calculus Bovis grinds; Get 5 flavors such as Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae, mix homogeneously is divided into 5 parts, in the sand hill that the warp layer cloth of packing into is made, add the water that is equivalent to 10 times of crude drug amounts, soaked vapor extraction volatile oil 1 hour, filter, collect volatile oil, obtain the 28L oil water mixture; Filtrate and filtering residue keep in Dark Place, and be standby; With collect the volatile oil oil water mixture add the beta-cyclodextrin inclusion compound contain 17kg, 50 ℃ of water-bath high shears disperseed enclose 4 hours, shear rate is 13000rm ^-1, get volatile oil beta-cyclodextrin inclusion compound liquid; Water extracts such as Radix Angelicae Sinensis decompressions low temperature is recycled to dosage, adds 95% ethanol (V/V) and is stirred to that to contain the alcohol amount be 60% (V/V), and cold preservation was placed 24 hours, filter, water extract-alcohol precipitation liquid keeps in Dark Place; Get Flos Carthami and add 30 times of water that are equivalent to the crude drug amount, decoct 3 times, each 2 hours, filter, filtrate is recycled to dosage, keeps in Dark Place; Filtering residues such as Flos Carthami filtering residue and Radix Angelicae Sinensis merge 70% ethanol (V/V) that adds 10 times of amounts of crude drug amount, heating and refluxing extraction 3 times, and each 3 hours, filter, water extracts such as alcohol extract and Radix Angelicae Sinensis merge recovery ethanol to dosage; 70% ethanol (V/V) that the water intaking trematodiasis adds 1 times of crude drug amount soaked 24 hours, percolation, and percolation speed is 2.5ml/ minute, collects the percolate that is equivalent to 10 times of crude drug amounts, medicinal residues are flung to ethanol, and are standby; Percolate reclaims ethanol 2 times to dosage, adds the beta-schardinger dextrin-saturated aqueous solution that contains 1.14kg, 60 ℃ of stirring in water bath 1.5 hours, and mixing speed is that 1000rpm gets Hirudo beta-cyclodextrin inclusion compound liquid, keeps in Dark Place; Get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge to add the decocting that is equivalent to 4 times of crude drug amounts and boil 3 times, each 2 hours, filter; Get Cornu Naemorhedi and add the decocting be equivalent to 10 times of crude drug amounts and boil 3 times, each 2 hours, filter filtrate for later use; The Cornu Naemorhedi medicinal residues add the 0.25%NaOH (W/V) that is equivalent to 8 times of crude drug amounts, and heating hydrolysis 4 hours keeps the skin wet, and filter, and alkaline hydrolyzate adds rare HCL and transfers pH to 7.0, keeps in Dark Place; Get In vitro cultured Calculus Bovis, be ground to fine powder, put in 30 times of water high shear and pulverized 3 hours, speed is 22000rpm.Merge water extracts such as alcohol extracts such as water extract-alcohol precipitation liquid, saffron aqueous solution, Radix Angelicae Sinensis, Hirudo, Cornu Naemorhedi extracting solution, volatile oil beta-cyclodextrin inclusion compound aqueous solution, Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution, In vitro cultured Calculus Bovis pulverizing liquid such as Radix Angelicae Sinensis; mix homogeneously; the sample solution that takes a morsel records mixed liquor solid content 0.0682g/g; density is 1.004g/ml, advances the spray drying tower drying.Inlet temperature: 160 ℃, outlet temperature: 82 ℃, obtain dry extract, mix homogeneously.Discharging dry extract 8.11kg after the spray drying.The rate of extract is 26.13% (W/W), moisture content 1.34% (W/V).Press dry extract 42%, crospolyvinylpyrrolidone (XL) 18%, mannitol 3.5%, anhydrous citric acid 7%, sodium bicarbonate 12%, microcrystalline Cellulose (PH102) 10%, micropowder silica gel 3.5%, magnesium stearate 0.4%, Pulvis Talci 1.8%, the ratio mixing of Aspartane 0.5%, direct compression is a coating material with OPADRY II (85G60989 BLUE), prepare into 18% water coating solution, inlet temperature: 50 ℃, the sheet bed tempertaure: 42 ℃, atomizing pressure: 3bar, coating pan rotating speed: 6～8rpm, coating weightening finish 3%, bag blue membrane clothing, promptly.

The cerebral thrombosis effervescent tablet disintegration time that embodiment 19. embodiment 11 make

The effervescent tablet disintegration time is as follows:

Effervescent timetable behind table 43 coating:

Lot number	Disintegration time
		050203-1 050203-2 050203-3 050203-4	6′05″ 7′34″ 6′31″ 6′04″

Claims (6)

1. cerebral thrombosis effervescent tablet for the treatment of apoplexy, it is characterized in that: wherein raw material consists of:

Flos Carthami 900g Radix Angelicae Sinensis 900g Hirudo 900g Radix Paeoniae Rubra 900g

Semen Persicae 900g Rhizoma Chuanxiong 900g Radix Salviae Miltiorrhizae 900g Eupolyphaga Seu Steleophaga 225g

Cornu Naemorhedi or Cornu Bubali 600～1500g In vitro cultured Calculus Bovis, Calculus Bovis cultivating or artificial Calculus Bovis 3～30g method for making: prepare by the following step:

(1) pulverize above-mentioned raw material of Chinese medicine standby;

(2) get Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae 5 flavors, add entry, soak, vapor extraction volatile oil gets oil-water mixture; Filtrate and filtering residue are standby;

(3) in step (2), collect oil-water mixture in add the inclusion agents enclose, inclusion agents can be used beta-schardinger dextrin-, disperses enclose to get inclusion essential oil liquid;

(4) water that step (2) is obtained is proposed the recovery of mother solution decompression low temperature, concentrated liquid measure after the recovery equals the dosage of Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae 5 flavor raw material of Chinese medicine, adds ethanol and is stirred to that to contain the alcohol amount be 55～85%V/V, and cold preservation is placed, filter, get water extract-alcohol precipitation liquid;

(5) get Flos Carthami adding decocting and boil 1～3 time, filter, filtrate is recycled to the dosage of Flos Carthami;

(6) Flos Carthami filtering residue that obtains in the step (5) and the filtering residue in the step (2) merge, add the 50～90%V/V ethanol that is equivalent to 6～10 times of amounts of this crude drug amount, heating and refluxing extraction 1～3 time, filter, water extract-alcohol precipitation liquid in alcohol extract and the step (4) merges, and reclaims ethanol to dosage and obtains alcohol extract;

(7) the water intaking trematodiasis adds soak with ethanol, and percolation is collected percolate, and it is standby that medicinal residues are flung to ethanol;

(8) percolate reclaims ethanol 1～3 times to dosage, adds the inclusion agents enclose and obtains Hirudo enclose liquid;

(9) get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge, add decocting and boil 1～3 time, filter, filtrate is recycled to dosage;

(10) get Cornu Naemorhedi or Cornu Bubali adding decocting and boil 3 times, filter filtrate for later use;

(11) medicinal residues of Cornu Naemorhedi or Cornu Bubali add the 0.25%W/V sodium hydroxide solution, heating hydrolysis filters, and alkaline hydrolyzate adds rare HCl and transfers pH to 7.0, the Cornu Naemorhedi that obtains with step (10) or the water extract of Cornu Bubali merge, and are recycled to the extracting solution that dosage obtains Cornu Naemorhedi or Cornu Bubali;

(12) get In vitro cultured Calculus Bovis, Calculus Bovis cultivating or artificial Calculus Bovis and add entry, high shear disperses to pulverize;

(13) alcohol extract of the inclusion essential oil aqueous solution that obtains of combining step (3), saffron aqueous solution that step (5) obtains, Radix Angelicae Sinensis that step (6) obtains, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae, Flos Carthami 6 flavor medicines, Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution that step (8) obtains, Eupolyphaga Seu Steleophaga that step (9) obtains and Hirudo water extract, Cornu Naemorhedi or the extracting solution of Cornu Bubali and the Calculus Bovis pulverizing liquid that step (12) obtains that step (11) obtains, add an amount of flavoring orange essence again, drying gets the dry extract powder;

(14) method and the adjuvant of the routine of use preparation effervescent tablet are made effervescent tablet with the dry extract that step (13) obtains.

2. according to the effervescent tablet of claim 1, it is characterized in that:

Raw material consists of:

Flos Carthami 900g Radix Angelicae Sinensis 900g Hirudo 900g Radix Paeoniae Rubra 900g

Semen Persicae 900g Rhizoma Chuanxiong 900g Radix Salviae Miltiorrhizae 900g Eupolyphaga Seu Steleophaga 225g

Cornu Naemorhedi or Cornu Bubali 900～1200g In vitro cultured Calculus Bovis or Calculus Bovis cultivating 6～18g method for making: prepare by the following step:

(1) Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae 4 are distinguished the flavor of and be ground into the powder of 10～50 mesh sieves, peach kernel powder was broken into the powder of 10～30 mesh sieves, and Flos Carthami, Hirudo, Eupolyphaga Seu Steleophaga 3 flavors were pulverized 20 orders, the Cornu Naemorhedi coarse pulverization, and In vitro cultured Calculus Bovis pulverizes, and is standby;

(2) get Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae 5 flavors, add the water that is equivalent to 6～10 times of this crude drug amounts, soaked≤2 hours, vapor extraction volatile oil 3～9 hours filters, and gets oil-water mixture, and water carries mother solution and filtering residue keeps in Dark Place, and is standby;

(3) in step (2), collect oil-water mixture in add the beta-cyclodextrin inclusion compound agent of 70g～120g, 40～60 ℃ of water-baths adopt high shear process to disperse enclose 2～4 hours, shear rate is 1000～13000rpm, volatile oil beta-cyclodextrin inclusion compound liquid;

(4) water that step (2) is obtained is carried mother solution decompression low temperature and is recycled to dosage, adds ethanol and is stirred to and contains the alcohol amount and be 60-70%V/V, and cold preservation is placed, filter, water extract-alcohol precipitation liquid keeps in Dark Place;

(5) get Flos Carthami and add 20～30 times of water that are equivalent to Flos Carthami crude drug amount, decoct 1～3 time, each 1～3 hour, filter, filtrate is recycled to dosage, keeps in Dark Place;

(6) the Flos Carthami filtering residue of step (5) and the filtering residue of step (2) are merged, 60～80%V/V the ethanol that adds 6～10 times of amounts of crude drug amount, heating and refluxing extraction 1～3 time, each 1～3 hour, filter, the water extract-alcohol precipitation liquid that this alcohol extract and step (4) obtain merges, and reclaims ethanol to dosage, obtains alcohol extract;

(7) the water intaking trematodiasis adds the 70%V/V soak with ethanol 24 hours of 1 times of crude drug amount, and percolation is collected percolate, and medicinal residues are flung to ethanol, and are standby;

(8) percolate reclaims ethanol 2 times to dosage, adds the agent of 300～450g beta-cyclodextrin inclusion compound, and 60 ℃ of stirring in water bath, mixing speed are that 1000rpm gets Hirudo beta-cyclodextrin inclusion compound liquid, keep in Dark Place;

(9) get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge, add the decocting that is equivalent to 3～5 times of crude drug amounts and boil 1～3 time, each 1～2 hour, filter, filtrate is recycled to dosage, preserves;

(10) get Cornu Naemorhedi or Cornu Bubali and add the decocting be equivalent to 10 times of crude drug amounts and boil 3 times, each 2 hours, filter filtrate for later use;

(11) medicinal residues of Cornu Naemorhedi or Cornu Bubali add the 0.25%W/V sodium hydroxide solution that is equivalent to 8 times of crude drug amounts, and heating hydrolysis 4 hours filters, alkaline hydrolyzate adds rare HCl and transfers pH to 7.0, merge with the water extract of Cornu Naemorhedi or Cornu Bubali, be recycled to dosage, keep in Dark Place;

(12) get In vitro cultured Calculus Bovis or Calculus Bovis cultivating, add the water of 30 times of amounts, high shear disperses to pulverize 2 hours, and speed is 22000～28000rpm;

(13) extracting solution of the volatile oil beta-cyclodextrin inclusion compound aqueous solution that obtains of combining step (3), saffron aqueous solution that step (5) obtains, Radix Angelicae Sinensis that step (6) obtains, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae, Flos Carthami 6 flavor medicine alcohol extracts, Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution that step (8) obtains, water extract that step (9) obtains, Cornu Naemorhedi that step (11) obtains or Cornu Bubali, the Calculus Bovis that obtains with step (12) are pulverized liquid, add an amount of flavoring orange essence again, spray drying, inlet temperature: 160 ℃, outlet temperature: 82 ℃, get the dry extract powder;

(14) press dry extract 30～42%, crospolyvinylpyrrolidone 10～30%, mannitol 3～15%, anhydrous citric acid 6～12%, sodium bicarbonate 10～18%, microcrystalline Cellulose 5～16%, micropowder silica gel 2～3.5%, magnesium stearate 0.4%, Pulvis Talci 1.8%, the ratio mixing of Aspartane 0.5%, direct compression.

3. according to the effervescent tablet of claim 2, it is characterized in that:

Raw material consists of:

Flos Carthami 900g Radix Angelicae Sinensis 900g Hirudo 900g Radix Paeoniae Rubra 900g

Semen Persicae 900g Rhizoma Chuanxiong 900g Radix Salviae Miltiorrhizae 900g Eupolyphaga Seu Steleophaga 225g

Cornu Naemorhedi 1125g In vitro cultured Calculus Bovis 12.5g

Method for making: prepare by the following step:

(1) Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae 4 are distinguished the flavor of and be ground into the fine powder of 50 mesh sieves, peach kernel powder was broken into the fine powder of 30 mesh sieves, and Flos Carthami, Hirudo, Eupolyphaga Seu Steleophaga 3 flavors were pulverized 20 orders, the Cornu Naemorhedi coarse pulverization, and In vitro cultured Calculus Bovis pulverizes, and is standby;

(2) get Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae 5 flavors, add the water that is equivalent to 10 times of crude drug amounts, soaked 1 hour, vapor extraction volatile oil filters, and gets oil-water mixture; Water carries mother solution and filtering residue keeps in Dark Place, and is standby;

(3) in step (2), collect oil-water mixture in add the beta-schardinger dextrin-of 93.3g, 50 ℃ of water-bath high shears disperseed enclose 4 hours, shear rate is 1300rpm, volatile oil beta-cyclodextrin inclusion compound liquid;

(4) water that step (2) is obtained is carried mother solution decompression low temperature and is recycled to dosage, adds 95%V/V ethanol and is stirred to and contains the alcohol amount and be 60%V/V, and cold preservation was placed 24 hours, filter, water extract-alcohol precipitation liquid, keep in Dark Place;

(5) get Flos Carthami and add 30 times of water that are equivalent to the crude drug amount, decoct 2 times, each 1 hour, filter, filtrate is recycled to dosage and obtains saffron aqueous solution, keeps in Dark Place;

(6) the Flos Carthami filtering residue of step (5) and the filtering residue of step (2) are merged, add the 70%V/V ethanol of 10 times of amounts of crude drug amount, heating and refluxing extraction 3 times, each 3 hours, filter, the water extract-alcohol precipitation liquid that this alcohol extract and step (4) obtain merges, reclaim ethanol to dosage, obtain alcohol extract;

(7) the water intaking trematodiasis adds the 70%V/V soak with ethanol 24 hours of 1 times of crude drug amount, percolation, and percolation speed is 2.5ml/ minute, collects the percolate that is equivalent to 10 times of crude drug amounts, medicinal residues are flung to ethanol, and are standby;

(8) percolate reclaims ethanol 2 times to dosage, adds the 302.8g beta-schardinger dextrin-, 60 ℃ of stirring in water bath 1.5 hours, and mixing speed is that 1000rpm gets Hirudo beta-cyclodextrin inclusion compound liquid, keeps in Dark Place;

(9) get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge, add the decocting that is equivalent to 4 times of crude drug amounts and boil 3 times, each 2 hours, filter, filtrate is recycled to dosage, preserves;

(10) get Cornu Naemorhedi and add the decocting be equivalent to 10 times of crude drug amounts and boil 3 times, each 2 hours, filter filtrate for later use;

(11) the Cornu Naemorhedi medicinal residues that step (10) obtained add the 0.25%W/V sodium hydroxide solution that is equivalent to 8 times of crude drug amounts, heating hydrolysis 4 hours, keep the skin wet, filter, alkaline hydrolyzate adds rare HCL and transfers pH to 7.0, merge with the Cornu Naemorhedi water extract of step (10), be recycled to dosage and obtain the Cornu Naemorhedi extracting solution, keep in Dark Place;

(12) get In vitro cultured Calculus Bovis, add the water of 30 times of amounts, high shear disperses to pulverize 2 hours, and speed is 22000rmp;

(13) Calculus Bovis that obtains of the volatile oil beta-cyclodextrin inclusion compound aqueous solution that obtains of combining step (3), saffron aqueous solution that step (5) obtains, alcohol extract that step (6) obtains, Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution that step (8) obtains, water extract that step (9) obtains, Cornu Naemorhedi extracting solution that step (11) obtains and step (12) is pulverized liquid, add an amount of flavoring orange essence again, spray drying, inlet temperature: 160 ℃, outlet temperature: 82 ℃, rotating speed: 21000rpm, power: 350HZ gets the dry extract powder;

(14) press dry extract 39%, crospolyvinylpyrrolidone 15%, mannitol 3.5%, anhydrous citric acid 9.94%, sodium bicarbonate 15.06%, microcrystalline Cellulose 11.8%, micropowder silica gel 3%, magnesium stearate 0.4%, Pulvis Talci 1.8%, the ratio mixing of Aspartane 0.5%, direct compression is a coating material with OPADRY II, prepare into 18% water coating solution, inlet temperature: 50 ℃, the sheet bed tempertaure: 42 ℃, atomizing pressure: 3bar, coating pan rotating speed: 6～8rpm, coating weightening finish 3%, bag blue membrane clothing, promptly.

4. according to the preparation method of the effervescent tablet of claim 1, comprise the following steps:

(1) raw material of Chinese medicine is pulverized standby;

(2) get Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae 5 flavors, add the water logging bubble, vapor extraction volatile oil gets oil-water mixture, and water carries mother solution and filtering residue is standby;

(3) in step (2), collect oil-water mixture in add inclusion agents, disperse enclose to get inclusion essential oil liquid;

(4) water that step (2) is obtained is carried mother solution decompression low temperature and is reclaimed, and the concentrated liquid measure after the recovery equals the dosage of this 5 flavor raw material of Chinese medicine, adds ethanol and is stirred to that to contain the alcohol amount be 55～85%V/V, and cold preservation is placed, filter, water extract-alcohol precipitation liquid;

(5) get Flos Carthami adding decocting and boil 1～3 time, filter, filtrate is recycled to dosage;

(6) Flos Carthami filtering residue that obtains in the step (5) and the filtering residue in the step (2) merge, add the 50～90%V/V ethanol that is equivalent to 6～10 times of amounts of this crude drug amount, heating and refluxing extraction 1～3 time, filter, water extract-alcohol precipitation liquid in alcohol extract and the step (4) merges, and reclaims ethanol to dosage and obtains alcohol extract;

(7) the water intaking trematodiasis adds soak with ethanol, and percolation is collected percolate, and it is standby that medicinal residues are flung to ethanol;

(8) percolate reclaims ethanol 1～3 times to dosage, adds the inclusion agents enclose and obtains Hirudo enclose liquid;

(9) get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge, add decocting and boil 1～3 time, filter, filtrate is recycled to dosage;

(10) get Cornu Naemorhedi or Cornu Bubali adding decocting and boil 3 times, filter filtrate for later use;

(11) Cornu Naemorhedi or Cornu Bubali medicinal residues add the 0.25%W/V sodium hydroxide solution, heating hydrolysis filters, and alkaline hydrolyzate adds rare HCl and transfers pH to 7.0, the Cornu Naemorhedi or the Cornu Bubali water extract that obtain with step (10) merge, and are recycled to dosage and obtain Cornu Naemorhedi or Cornu Bubali extracting solution;

(12) get In vitro cultured Calculus Bovis, Calculus Bovis cultivating or artificial Calculus Bovis and add entry, high shear disperses to pulverize;

(13) the inclusion essential oil aqueous solution that obtains of combining step (3), saffron aqueous solution that step (5) obtains, Radix Angelicae Sinensis that step (6) obtains, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae, Flos Carthami 6 flavor medicine alcohol extracts, Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution that step (8) obtains, Eupolyphaga Seu Steleophaga that step (9) obtains and Hirudo water extract, Cornu Naemorhedi or the extracting solution of Cornu Bubali and the Calculus Bovis pulverizing liquid that step (12) obtains that step (11) obtains, add an amount of flavoring orange essence again, drying gets the dry extract powder;

(14) method and the adjuvant of the routine of use preparation effervescent tablet are made effervescent tablet with the dry extract that step (13) obtains.

5. according to the preparation method of the effervescent tablet of claim 2, comprise the following steps:

(1) Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae 4 are distinguished the flavor of and be ground into the powder of 10～50 mesh sieves, peach kernel powder was broken into the powder of 10～30 mesh sieves, and Flos Carthami, Hirudo, Eupolyphaga Seu Steleophaga 3 flavors were pulverized 20 orders, the Cornu Naemorhedi coarse pulverization, and In vitro cultured Calculus Bovis pulverizes, and is standby;

(2) get Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae 5 flavors, add the water that is equivalent to 6～10 times of crude drug amounts, soaked 0～2 hour, vapor extraction volatile oil 3～hour, filter, get oil-water mixture, water carries mother solution and filtering residue keeps in Dark Place, and is standby;

(3) in step (2), collect oil-water mixture in add the beta-cyclodextrin inclusion compound agent of 70g～120g, 40～60 ℃ of water-baths adopt high shear process to disperse enclose 2～4 hours, shear rate is 1000～13000rpm, volatile oil beta-cyclodextrin inclusion compound liquid;

(4) water that step (2) is obtained is carried mother solution decompression low temperature and is recycled to dosage, adds ethanol and is stirred to and contains the alcohol amount and be 60-70%V/V, and cold preservation is placed, filter, water extract-alcohol precipitation liquid, keep in Dark Place;

(5) get Flos Carthami and add 20～30 times of water that are equivalent to Flos Carthami crude drug amount, decoct 1～3 time, each 1～3 hour, filter, filtrate is recycled to dosage, keeps in Dark Place;

(6) the Flos Carthami filtering residue of step (5) and the filtering residue of step (2) are merged, 60～80%V/V the ethanol that adds 6～10 times of amounts of crude drug amount, heating and refluxing extraction 1～3 time, each 1～3 hour, filter, the water extract-alcohol precipitation liquid that this alcohol extract and step (4) obtain merges, and reclaims ethanol to dosage, obtains alcohol extract;

(7) the water intaking trematodiasis adds the 70%V/V soak with ethanol 24 hours of 1 times of crude drug amount, and percolation is collected percolate, and medicinal residues are flung to ethanol, and are standby;

(8) percolate reclaims ethanol 2 times to dosage, adds the agent of 300～450g beta-cyclodextrin inclusion compound, and 60 ℃ of stirring in water bath, mixing speed are that 1000rpm gets Hirudo beta-cyclodextrin inclusion compound liquid, keep in Dark Place;

(9) get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge, add the decocting that is equivalent to 3～5 times of crude drug amounts and boil 1～3 time, each 1～2 hour, filter, filtrate is recycled to dosage, preserves;

(10) get Cornu Naemorhedi or Cornu Bubali and add the decocting be equivalent to 10 times of crude drug amounts and boil 3 times, each 2 hours, filter filtrate for later use;

(11) Cornu Naemorhedi or Cornu Bubali medicinal residues add the 0.25%W/V sodium hydroxide solution that is equivalent to 8 times of crude drug amounts, and heating hydrolysis 4 hours filters, and alkaline hydrolyzate adds rare HCl and transfers pH to 7.0, merge with Cornu Naemorhedi or Cornu Bubali water extract, are recycled to dosage, keep in Dark Place;

(12) get In vitro cultured Calculus Bovis or Calculus Bovis cultivating, add the water of 30 times of amounts, high shear disperses to pulverize 2 hours, and speed is 22000～28000rpm;

(13) the volatile oil beta-cyclodextrin inclusion compound aqueous solution that obtains of combining step (3), the saffron aqueous solution that step (5) obtains, the Radix Angelicae Sinensis that step (6) obtains, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae, the alcohol extract of Flos Carthami 6 flavor medicines, the Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution that step (8) obtains, the water extract that step (9) obtains, the Cornu Naemorhedi that step (11) obtains or the extracting solution of Cornu Bubali, pulverize liquid with the Calculus Bovis that step (12) obtains, add an amount of flavoring orange essence again, spray drying, inlet temperature: 160 ℃, outlet temperature: 82 ℃, get the dry extract powder;

(14) press dry extract 30～42%, crospolyvinylpyrrolidone 10～30%, mannitol 3～15%, anhydrous citric acid 6～12%, sodium bicarbonate 10～18%, microcrystalline Cellulose 5～16%, micropowder silica gel 2～35%, magnesium stearate 0.4%, Pulvis Talci 1.8%, the ratio mixing of Aspartane 0.5%, direct compression.

6. require the preparation method of 3 effervescent tablet according to power, comprise the following steps:

(1) Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae 4 are distinguished the flavor of and be ground into the fine powder of 50 mesh sieves, peach kernel powder was broken into the fine powder of 30 mesh sieves, and Flos Carthami, Hirudo, Eupolyphaga Seu Steleophaga 3 flavors were pulverized 20 orders, the Cornu Naemorhedi coarse pulverization, and In vitro cultured Calculus Bovis pulverizes, and is standby;

(2) get Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Semen Persicae 5 flavors, add the water that is equivalent to 10 times of crude drug amounts, soaked 1 hour, vapor extraction volatile oil filters, and gets oil-water mixture; Water carries mother solution and filtering residue keeps in Dark Place, and is standby:

(3) in step (2), collect oil-water mixture in add the beta-schardinger dextrin-of 93.3g, 50 ℃ of water-bath high shears disperseed enclose 4 hours, shear rate is 13000rpm, volatile oil beta-cyclodextrin inclusion compound liquid;

(4) water that step (2) is obtained is carried mother solution decompression low temperature and is recycled to dosage, adds 95%V/V ethanol and is stirred to and contains the alcohol amount and be 60%V/V, and cold preservation was placed 24 hours, filter, water extract-alcohol precipitation liquid, keep in Dark Place;

(5) get Flos Carthami and add 30 times of water that are equivalent to the crude drug amount, decoct 2 times, each 1 hour, filter, filtrate is recycled to dosage and obtains saffron aqueous solution, keeps in Dark Place;

(6) the Flos Carthami filtering residue of step (5) and the filtering residue of step (2) are merged, add the 70%V/V ethanol of 10 times of amounts of crude drug amount, heating and refluxing extraction 3 times, each 3 hours, filter, the water extract-alcohol precipitation liquid that this alcohol extract and step (4) obtain merges, reclaim ethanol to dosage, obtain alcohol extract;

(7) the water intaking trematodiasis adds the 70%V/V soak with ethanol 24 hours of 1 times of crude drug amount, percolation, and percolation speed is 2.5ml/ minute, collects the percolate that is equivalent to 10 times of crude drug amounts, medicinal residues are flung to ethanol, and are standby;

(8) percolate reclaims ethanol 2 times to dosage, adds the 302.8g beta-schardinger dextrin-, 60 ℃ of stirring in water bath 1.5 hours, and mixing speed is that 1000rprm gets Hirudo beta-cyclodextrin inclusion compound liquid, keeps in Dark Place;

(9) get Eupolyphaga Seu Steleophaga and Hirudo medicinal residues and merge, add the decocting that is equivalent to 4 times of crude drug amounts and boil 3 times, each 2 hours, filter, filtrate is recycled to dosage, preserves;

(10) get Cornu Naemorhedi and add the decocting be equivalent to 10 times of crude drug amounts and boil 3 times, each 2 hours, filter filtrate for later use;

(11) the Cornu Naemorhedi medicinal residues that step (10) obtained add the 0.25%W/V sodium hydroxide solution that is equivalent to 8 times of crude drug amounts, heating hydrolysis 4 hours, keep the skin wet, filter, alkaline hydrolyzate adds rare HCL and transfers pH to 7.0, merge with the Cornu Naemorhedi water extract of step (10), be recycled to dosage and obtain the Cornu Naemorhedi extracting solution, keep in Dark Place;

(12) get In vitro cultured Calculus Bovis, add the water of 30 times of amounts, high shear disperses to pulverize 2 hours, and speed is 22000rmp;

(13) Calculus Bovis that obtains of the volatile oil beta-cyclodextrin inclusion compound aqueous solution that obtains of combining step (3), saffron aqueous solution that step (5) obtains, alcohol extract that step (6) obtains, Hirudo ethanol extract beta-cyclodextrin inclusion compound aqueous solution that step (8) obtains, water extract that step (9) obtains, Cornu Naemorhedi extracting solution that step (11) obtains and step (12) is pulverized liquid; add an amount of flavoring orange essence again; spray drying; inlet temperature: 160 ℃; outlet temperature: 82 ℃; rotating speed: 21000rpm; power: 350HZ gets the dry extract powder.

(14) press dry extract 39%, crospolyvinylpyrrolidone 15%, mannitol 3.5%, anhydrous citric acid 9.94%, sodium bicarbonate 15.06%, microcrystalline Cellulose 11.8%, micropowder silica gel 3%, magnesium stearate 0.4%, Pulvis Talci 1.8%, the ratio mixing of Aspartane 0.5%, direct compression is a coating material with OPADRY II, prepare into 18% water coating solution, inlet temperature: 50 ℃, the sheet bed tempertaure: 42 ℃, atomizing pressure: 3bar, coating pan rotating speed: 6～8rpm, coating weightening finish 3%, bag blue membrane clothing, promptly.