CN100348212C - Chinese medicine capsule used for regulating immunity, its preparation method and use - Google Patents
Chinese medicine capsule used for regulating immunity, its preparation method and use Download PDFInfo
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- CN100348212C CN100348212C CNB200510068349XA CN200510068349A CN100348212C CN 100348212 C CN100348212 C CN 100348212C CN B200510068349X A CNB200510068349X A CN B200510068349XA CN 200510068349 A CN200510068349 A CN 200510068349A CN 100348212 C CN100348212 C CN 100348212C
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Abstract
The present invention relates to a Chinese medicine capsule used for regulating immunity, a preparation method and use thereof. The capsule is made from the following raw materials with a weight ratio of 4 to 16 portions of rainbow conk powder after extraction, 3 to 12 portions of shiitake fungus powder after extraction and 3 to 12 portions of milk vetch powder after extraction. The preparation method comprises that the rainbow conk powder, the shiitake fungus powder and the milk vetch powder after extraction are respectively weighed and sieved by a sieve of 60 meshes. The materials are externally detected, mixed and packaged by a double-layer polyethylene plastic bag. The capsule is filled by a filling machine, and products are obtained after the capsule is polished, inspected, filled in bottles and packaged in boxes. The present invention has the advantages of high safety of traditional Chinese medicine capsules and no toxic side effect; the present invention is suitable for patients in delicate health and recovery people after prolonged illness. The product of the present invention can be used for enhancement of immunological functions, replenishing of blood, promotion of the production of the body fluid, antioxidation, anti-aging, radioresistance, nourishing and protection of the liver, reinforcement of physique disease resistance and promotion of physical rehabilitation.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition that is used for the treatment of disease, relate to Chinese medicinal capsule of regulating immunity and preparation method thereof specifically.
Background technology
Among the crowd of society, some long-term sufferers, physical weakness, suffer from wasting diseases (as pneumonopathy, gastroenteropathy, tumor, anemia, thyroid is hyperfunction, diabetes), and the patient after the treatment that undergos surgery, all demand is nourished nutrition, improve body resistance against diseases, improve health living accommodation ability and existence attainment: many tumors, the cancer patient is through chemotherapy, behind the radiotherapy, leukopenia appears, appetite is poor, vomiting, alopecia, body constitution is poor, resistance decline situation, have a strong impact on therapeutic effect and life quality, they need to replenish body immunity, reduce chemotherapy, the side effect of radiotherapy, improving appetite, antioxidation, radioprotective etc.; There is physical weakness in the patient after hepatitis, hepatocarcinoma, the hepatopathy, and appetite is poor, and demand strengthens health immune disease-resistance ability, improves body constitution, improves health digestion, hemopoietic metabolic function; Along with social personnel's age ageing, increasing health aging, weak hypoimmunity, normal easily ill crowd, the demand health invigorating, defying age, antioxidation improves immunologic function, disease resistance enhancemen.
Though it is many to nourish the kind of product on the market, function singleness, some of them are the scorching medicine of tonifying YANG, and non-chronically ill man suits.Therefore, it is a kind of improving immunologic function, enriching blood and promoting secretion of body fluid to be badly in need of research and development, antioxidation, defying age, and radioprotective, the nourishing the liver hepatoprotective, the health invigorating resistance against diseases promotes multi-functional, the pure natural preparation that effect is gentle of physical rehabilitation, is numerous patients' needs.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art part, utilize existing medical science new development and provide, press Chinese medical theory and modern medicine study, adopt suitable compatible combination, collaborative playing a role, provide an energy comprehensive, have enhance immunity ability, defying age, protect the liver, mend effects such as needing QI invigorating at a plurality of links a kind of, composition capsule (trade name: give birth to section and mend precious (RVF)) that becomes with natural drug component and the homologous prepared from traditional Chinese medicines of medicine and preparation method thereof.
Purpose of the present invention can realize by following measure: this capsule is to be made by the raw material of following weight ratio:
Coriolous Dersicolor (Fr.) Quel powder 4-16 part after the extraction
Lentinus Edodes powder 3-12 part after the extraction
Astragalus membranaceus powder 3-12 part after the extraction.
Preferred proportioning raw materials is:
12 parts in Coriolous Dersicolor (Fr.) Quel powder after the extraction
9 parts in Lentinus Edodes powder after the extraction
9 parts of astragalus membranaceus powders after the extraction.
The preparation method that the present invention also provides this life section to mend precious composition capsule:
The preparation method of the Coriolous Dersicolor (Fr.) Quel powder after the extraction: get qualified Coriolous Dersicolor (Fr.) Quel, clean, the removal of impurity, clear water is cleaned, and chopping is put in the multi-function extractor, add 8-12 times of water gaging, be preferably 10 times, boil, extract 2-4 time, be preferably 3 times, boil extraction time to be 1.5-2.5 hour at every turn, be preferably 2 hours, extracting solution 200 orders are crossed screen cloth, get filtrate,-80--95kpa, 65-80 ℃ of decompression concentrates, 60 ℃ of following relative densities are 1.08-1.10, cross 120 orders, merge concentrated solution, be chilled to room temperature, stir the ethanol of slow adding 95% down, make the alcohol amount that contains of concentrated solution clear paste reach 85%, stir, left standstill 24 hours, filter, get the precipitation drying under reduced pressure, be ground into smalls, promptly make the Coriolous Dersicolor (Fr.) Quel powder after the extraction.
Lentinus Edodes after the extraction, the preparation method of the Radix Astragali: get qualified Lentinus Edodes, the Radix Astragali respectively, clean, the removal of impurity, clear water is cleaned, and chopping is put in the multi-function extractor, add 8-12 times of water gaging, be preferably 10 times, boil, extract 2-4 time, be preferably 3 times, boil extraction time to be 0.5-1.5 hour at every turn, be preferably 1 hour, extracting solution 200 orders are crossed screen cloth, get filtrate ,-80--95kpa, 65-80 ℃ of decompression, concentrate, 60 ℃ of following relative densities are 1.05-1.08, cross 120 orders, merge concentrated solution, spray drying makes Lentinus Edodes powder after the extraction respectively, the astragalus membranaceus powder after extracting.
By proportioning raw materials of the present invention, take by weighing respectively and extract back Coriolous Dersicolor (Fr.) Quel powder, extract back Lentinus Edodes powder, extract the back astragalus membranaceus powder, it is stand-by to cross 60 mesh sieves; All raw materials carry out outward appearance and detect, and place and mix powder in the mixer, collect the mixed powder through mix homogeneously, usefulness double-layer polyethylene (PE) poly-bag packed for standby use; The mixed powder of mix homogeneously is carried out capsule charge, capsule polishing, checks in automatic or semi-automatic capsule filler, go into bottle, go into the box packing, promptly get product.
The present invention has following advantage compared to existing technology: Chinese medicinal capsule safety of the present invention is good, has no side effect, and is applicable to physical weakness, is back on one's feet after a long illness and is used for dietetic therapy and prevention and health care that chronic hepatitis B, carninomatosis rehabilitation and old immunologic function reduce the crowd.
The specific embodiment
Following listed examples is further specified the present invention, but does not limit the present invention in any way.
The preparation of raw material:
Get qualified Coriolous Dersicolor (Fr.) Quel, clean, the removal of impurity, clear water is cleaned, and chopping is put in the multi-function extractor, add 10 times of water gagings, boil, extract 3 times, boil extraction time is 2 hours at every turn, and extracting solution 200 orders are crossed screen cloth, get filtrate,-80--95kpa, 65-80 ℃ of decompression concentrates, 60 ℃ of following relative densities are 1.08-1.10, cross 120 orders, merge concentrated solution, be chilled to room temperature, stir down the slowly ethanol of adding 95%, make the alcohol amount 85% that contains of concentrated solution clear paste, stir, left standstill 24 hours, and filtered, get the precipitation drying under reduced pressure, be ground into smalls, promptly make the Coriolous Dersicolor (Fr.) Quel powder after the extraction.
Get qualified Lentinus Edodes, the Radix Astragali respectively, clean, the removal of impurity, clear water is cleaned, chopping is put in the multi-function extractor, adds 10 times of water gagings, boil, extract 3 times, boil extraction time to be 1 hour at every turn, extracting solution 200 eye mesh screens filter, and get filtrate ,-80--95kpa, 65-80 ℃ of decompression concentrates, and 60 ℃ of relative densities are that 1.05-1.08 crosses 120 orders, merge concentrated solution, spray drying makes Lentinus Edodes powder after the extraction respectively, the astragalus membranaceus powder after extracting.
Embodiment 1
Present embodiment is by following component: take by weighing the Coriolous Dersicolor (Fr.) Quel powder 1.2kg after each component raw material is extracted respectively, Lentinus Edodes powder 0.9kg after the extraction, astragalus membranaceus powder 0.9kg after the extraction, each raw material is sieved through 60 eye mesh screens, get each raw material components that sieves qualified, put and mix powder in the mixer, collection is through the mixed powder of mix homogeneously, with double-layer polyethylene (PE) poly-bag packed for standby use, the mixed powder of mix homogeneously is carried out capsule charge, capsule polishing, checks in automatic or semi-automatic capsule filler, go into bottle, go into the box packing and promptly get product.
Embodiment 2
Press the method for embodiment 1, wherein, with the Coriolous Dersicolor (Fr.) Quel powder 0.4kg after extracting, the Lentinus Edodes powder 0.3kg after the extraction, the astragalus membranaceus powder 0.3kg after the extraction replaces respectively that corresponding raw material makes among the embodiment 1.
Embodiment 3
Press the method for embodiment 1, wherein, with the Coriolous Dersicolor (Fr.) Quel powder 1.6kg after extracting, the Lentinus Edodes powder 1.2kg after the extraction, the astragalus membranaceus powder 1.2kg after the extraction replaces respectively that corresponding raw material makes among the embodiment 1.
The immunity effect of Chinese medicinal capsule of the present invention and nonhazardous effect.Medicine Pharmacology Lab experimentation proves through Guangzhou department of pharmacy of Zhongshan University: giving birth to precious (RVF) composition capsule of section's benefit has good pharmacologically active, has the effect that promotes body immunity, significantly improve the phagocytic rate and the phagocytic index of mouse macrophage, improve mouse immune organ index, the improve ability of learning and memory of mice of tool; Also to the D-galactose all have to subacute old and feeble rat and mice and improve memory ability significantly, remove the generation of intravital peroxide radical (MDA, MAO) preferably, improve the content of anti-oxidation active substance (SOD, GSH-Px) in the body, help cell membrane stability.
1, experiment material
1.1 trial drug
Give birth to section and mend precious (RVF), the khaki powder, the easy moisture absorption, lot number: 03001, the date of manufacture: 20031203; Provide by Hong Kong Biological Science and Technology Research Inst. Co., Ltd..
1.2 positive control drug:
QINGCHUNBAO PIAN, Zhejiang are defended No. the 0371st, the strong word (1996) of medicine, and Zhengda Qingchunbao Pharmaceutical Co., Ltd produces, lot number 0211016;
SHENQI JINGWU JIAONANG, the accurate word Z52020322 of traditional Chinese medicines number, Guizhou Jinqiao Pharmaceutical Co produces, lot number: 20030902.
1.3 experimental animal
The SD rat is provided by Guangdong Province's Experimental Animal Center, the animal quality certification number: 2002A024;
The NIH mice is provided by Guangdong Province's Experimental Animal Center, the animal quality certification number: 2002A022;
The experimental situation quality certification: Guangdong searching 2001C020 number.
1.4 reagent and medicine
Cyclophosphamide injection powder pin, Shanghai are defended the accurate word (1995) of medicine No. 012034, lot number: 030510, and 200mg/ props up; Hualian Pharmaceutical Co., Ltd., Shanghai produces;
The D-galactose, Shanghai permanent letter chemical reagent company limited is produced lot number: 20031030.
Citric acid trisodium, citric acid, glucose, sodium chloride, methanol, acetone etc., the AR level is purchased in the Guangzhou glass.
Chicken red blood cell (CRBC) is purchased live chickens from market, the preparation of sterile blood sampling Alsever ' s solution, autoclaving.
1.5 experimental apparatus
The rotary whole blood viscosity meter of LBY-N6A type self-cleaning, Beijing Puli gives birth to group and produces;
LBY-NM1 type self-cleaning blood plasma, serum viscosity in rabbits meter, Beijing Puli gives birth to group and produces;
The visible ultraviolet light spectrophotometer of UV-2102 type, Shanghai You Nika company limited is produced;
LBY-NJ2 type platelet aggregation instrument, Beijing Puli gives birth to group and produces;
YLS-2T type mice diving tower monitor, Shandong Academy of Medical Sciences equipment station produces;
The multi-functional mice automatic movable of YLS-1A type monitor, Shandong Academy of Medical Sciences equipment station produces;
1.6 dosage setting
The dosage of giving birth to section's benefit treasured and positive control drug is as shown in table 1 below.
Table 1 is pressed body surface area equivalent coefficient conversion dosage
| Group | Adult's dosage (g/ day/70kg) | Be equivalent to the multiple of being grown up | Rat dosage (g/kg) | Mice dosage (g/kg) | |
| Giving birth to section mends precious | Low dosage | 10 | 1 | 0.089 | 0.129 |
| Middle dosage | 3.0 | 3 | 0.267 | 0.387 | |
| High dose | 9.0 | 9 | 0.804 | 1.161 | |
| QINGCHUNBAO PIAN | 5.0 | 3 | 1.338 | 1.932 | |
| SHENQI JINGWU JIAONANG | 8.1 | 3 | 2.170 | 3.132 | |
The dosage design considerations:
Give birth to section and mend precious clinical plan to give the 70kg adult dosage that take every day be 1g, the dose,equivalent that is converted into rat by body surface area is 0.089g/kg, and the dose,equivalent that is converted into mice is 0.129g/kg.Positive control drug all adopts be equivalent to 70kg adult taking dose 3 times dosage in contrast, and therefore, QINGCHUNBAO PIAN Coming-of-Age Day taking dose is 5g, and the dose,equivalent that is converted into rat by body surface area is 0.446g/kg, and 3 multiple doses are 1.338g/kg; The dose,equivalent that is converted into mice is 0.644g/kg, and 3 multiple doses are 1.932g/kg.SHENQI JINGWU JIAONANG Coming-of-Age Day taking dose 8.1g, the dose,equivalent that is converted into rat by body surface area is 0.723g/kg, 3 multiple doses are 2.170g/kg; The dose,equivalent that is converted into mice is 1.044 g/kg, and 3 multiple doses are 3.132g/kg.
2, experimental technique
Mend precious acute toxicity testing 2.1 give birth to section
Mend the maximum tolerated dose experiment that a precious per os gives given the test agent 2.1.1 give birth to section
Select 40 of the healthy NIH mices of body weight 18-22g for use, male and female half and half, fasting be can't help water more than 12 hours before the experiment, every mice all once the heavy dose section of giving birth to mend precious suspension and maximum volume 0.8ml; Observed 7 days continuously after the administration, judge toxic reaction.Notice whether mice spirit occurred, activity, diet abnormal conditions and death condition in 7 day observation period.Mice for death should be dissected immediately, the perusal important organ heart, and liver, spleen, lung, whether kidneys etc. have obvious pathological changes.
Mend the precious maximum tolerated dose experiment that repeatedly gives given the test agent 2.1.2 give birth to section
Select 40 of the healthy NIH mices of body weight 18-22g for use, male and female half and half, fasting be can't help water more than 12 hours before the experiment, was administered once every 2 hours then, and the section of giving birth to mends precious suspension Cmax and only irritates stomach 0.5ml/; Whole day is total to administration 4 times.Observed 7 days continuously after the administration, judge toxic reaction, the record mice gives spirit whether to occur, activity, diet abnormal conditions and death condition during the given the test agent and in the observation period.For the mice of the death anatomic observation heart immediately, liver, spleen, lung, kidney etc.Calculate the maximum tolerated dose of given the test agent.
Section's benefit is precious to cause the low effect of immune function of mice to cyclophosphamide 2.2 give birth to
Select 60 of the healthy NIH mices of body weight 18-22g for use, be divided into six groups at random, promptly the normal control group is given birth to section and is mended precious basic, normal, high dosage group, positive controls and cyclophosphamide model control group, and 10 every group is male entirely.During the experiment beginning, remove the normal control group, all the other respectively organize mice every 3d intraperitoneal injection of cyclophosphamide 30mg/kg respectively, totally 3 times.Begin administration simultaneously, living section mends precious basic, normal, high dosage group mice gastric infusion every day dosage and is respectively 0.129g/kg, 0.387g/kg, 1.160g/kg, is equivalent to 1 times, 3 times, 9 times of clinical day taking dose 1g of 70kg adult; Normal control group and cyclophosphamide model control group are irritated stomach every day and are given normal saline with volume; It is 3.132g/kg that the positive control drug SHENQI JINGWU JIAONANG is irritated stomach dosage every day, is equivalent to 3 times of clinical day taking dose 8.1g of 70kg adult.
During two weeks, behind last administration 30min,, adopt YLS-2T type mice diving tower monitor to test its school grade and be the wrong number of times of appearance in the 5min with diving tower method training learning and memory abilities in aging mice; After 24 hours mice is placed on the diving tower, test memory achievement is that mice jumps off the incubation period of platform and the wrong total degree (n) in the 3min for the first time; Calculate diving tower mistake slip (%).
Diving tower mistake slip (%)=(model group errors number-administration group errors number) * 100%
In the time of around the administration, chicken red blood cell (CRBC) the suspension 1ml of every Mus abdominal cavity (being volume ratio 5%) takes off cervical vertebra and puts to death behind 4h, earlier the record body weight; Be fixed in then on the Mus plate, abdominal skin is cut off in the center, injects heparin sodium normal saline 2ml through the abdominal cavity, behind the soft mouse web portion, sucking-off peritoneal irrigation drop sheet push jack, dry the back and adopt methanol: acetone (1: 1) is fixedly behind the 5min, Giemsa-Wright dyeing, oily sem observation counting.Get spleen at last and thymus is weighed, calculate index and spleen index and thymus index.
Phagocytic rate %=engulfs macrophage/100 macrophage (gulp down and do not gulp down) * 100% of CRBC
Organ index=organ weight/body weight
The preparation of CRBC: under the sterile working, from the chicken venous blood collection, place 100ml vaccine bottle, add Alsever ' the s solution mixing that is equivalent to five times of Sanguis Gallus domesticus volumes, 4 ℃ of preservations are standby.
Alsever ' s solution: citric acid trisodium 8.0g, citric acid 0.5g, anhydrous glucose 18.7g, sodium chloride 4.2g is settled to 1000ml, packing, 4 ℃ of preservations are standby behind the autoclaving.
Mend precious effect 2.3 give birth to section to the subacute aging model of rat due to the D-galactose
Choose 60 of the male SD rats of body weight 300-400g, be divided into six groups at random, promptly the normal control group is given birth to section and is mended precious basic, normal, high dosage group, positive controls and D-galactose model control group, every group of 10 rats.Remove the normal control group, other respectively organizes rat lumbar injection every day D-galactose 100mg/kg, injects for six weeks continuously, the physiological saline solution of normal control group lumbar injection equivalent.Begin administration simultaneously, living section mends precious basic, normal, high dosage group rat gastric infusion every day dosage and is respectively 0.089g/kg, 0.267g/kg, 0.804g/kg, is equivalent to 1 times, 3 times, 9 times of clinical day taking dose 1g of 70kg adult; Normal control group and model control group are irritated stomach every day and are given normal saline with volume; It is 1.328g/kg that the positive control drug QINGCHUNBAO PIAN is irritated stomach dosage every day, is equivalent to 3 times of clinical day taking dose 5g of 70kg adult.
Successive administration carries out water maze laboratory after six weeks, measures the ability of learning and memory of rat; Get the hematometry hemorheology index then, comprise whole blood viscosity, plasma viscosity, serum viscosity in rabbits, Fibrinogen viscosity, platelet aggregation rate and erythrocyte inferior long-pending (HCT) or the like; Get peripheral blood and measure the content of MDA, GSH-Px, SOD and MAO; Put to death rat, weigh, get spleen, thymus and full brain and weigh, calculate index and spleen index, thymus index and cerebral index; Other gets the content that big brain homogenate is measured MDA, GSH-Px, SOD and MAO.Carry out statistical procedures and comparable group differences.
The water maze design: by diameter 150cm, the circle of high 60cm, black pond are formed, the dark 40cm of pool inner water, and water temperature keeps 26 ℃; Pool wall japanning adularescent or red symbol as triangle, circle, waveform and rectangle, and indicate four place of entry of East, West, South, North; The pond is divided into four equal quadrants, is respectively SW, NW, SE, NE quadrant, being placed with a diameter in SW quadrant center is 10cm, the circular movable black platform of high 39cm, and platform is lower than about horizontal plane 1cm.The position of object of reference remains unchanged outside the training period labyrinth.
Space learning recall tests: training earlier before the rat experiment, put into water from different quadrants every day, write down each Mus and find the platform required time with four difference entry respectively, be and look for platform incubation period that (Searching platform latency is SPL) as the learning and memory achievement, if rat is not found platform in 2min, rat can be lured on the platform, this moment, SPL pressed 120s calculating, and each 60s. at interval so trained 2-4 days, began to measure SPL value (being orientation navigation experiment).Removed underwater platform in the 5th day, optional water spot is put into water with rat towards pool wall, the record rat is after removing underwater platform, cross over original underwater platform relevant position number of times in the 120s, cross over the platform number of times (Crossing platform times, CPT) with and cross over the percent of all quadrants platform relevant position total degree, stride platform percentage rate (Crossingplatform percentages, CPP), detect rat space learning memory ability (being the space exploration experiment).
Mend precious effect 2.4 give birth to section to the subacute aging model of d-galactose induced mice
Select 60 of the male NIH mices of body weight 18-22g for use, be divided into six groups at random, promptly the normal control group is given birth to section and is mended precious basic, normal, high dosage group, positive controls and d-galactose model control group, every group of 10 mices.Remove the normal control group, all the other respectively organize the mice nape every day subcutaneous injection 0.5%D-of portion galactose, and 0.1ml/10g injected for six weeks continuously, the physiological saline solution of normal control group subcutaneous injection equivalent.。Begin administration simultaneously, living section mends precious basic, normal, high dosage group mice gastric infusion every day dosage and is respectively 0.129g/kg, 0.387g/kg, 1.160g/kg, is equivalent to 1 times, 3 times, 9 times of clinical day taking dose 1g of 70kg adult; Normal control group and model control group are irritated stomach every day and are given normal saline with volume; It is 1.932g/kg that the positive control drug QINGCHUNBAO PIAN is irritated stomach dosage every day, is equivalent to 3 times of clinical day taking dose 5g of 70kg adult.
Successive administration is after six weeks, measure the autonomic activities situation of respectively organizing mice, autonomic activities number of times in the record mice 5min, the improvement rate (%) of autonomic activities is calculated in the experiment back, adopt diving tower method training learning and memory abilities in aging mice then, write down its school grade and be the number of times that occurs mistake in the 5min; After 24 hours mice is placed on the diving tower, record memory achievement, both mice jumped off the incubation period and the 3 interior wrong total degrees (n) of platform for the first time; Calculate diving tower mistake slip (%); The comparable group differences also carries out statistical procedures.
Experiment is last, takes off cervical vertebra and puts to death mice, and the body weight of record mice is got thymus, spleen is weighed, and calculates thymus index and index and spleen index.
Autonomic activities improvement rate (%)=(administration group autonomic activities number of times-model group autonomic activities number of times) * 100%
Diving tower mistake slip (%)=(model group errors number-administration errors number) * 100%
3, experimental result
Mend precious acute toxicity testing result 3.1 give birth to section
Mend the experimental result that a precious per os gives the given the test agent maximal dose 3.1.1 give birth to section
Precious suspension Cmax is mended by the mouse stomach section of giving birth to, and (maxima solubility of this sample is 27.8%; Solution for the brown homogeneous) and behind the maximum volume 0.8ml, there was not the animal dead of appearance the same day; Observed 7 days continuously, the mice hair color, activity, diet is all normal, does not have dead the generation.Put to death animal, and dissected all no abnormal discoveries such as the perusal heart, liver, spleen, lung, kidney in the 8th day after the administration.Because administration volume and concentration can not increase again, it is 0.222g that precious maximal dose is mended by per os section of giving birth to of mice, be converted to the disposable oral living section of the adult of 70kg by body surface area and mend precious total amount and be no less than 86.3g, be equivalent to 86 times of adult's taking dose every day (1g/ day) of 70kg.
Mend the precious maximum tolerated dose experimental result that repeatedly gives given the test agent 3.1.2 give birth to section
Precious suspension Cmax is mended by the mice section of giving birth on the same day, and (maxima solubility of this sample is 27.8%; Solution for the brown homogeneous) 0.5ml/ time, none the routine dead mouse of back that is administered four times is altogether observed continuously and was seen that the mice hair color was smooth in 7 days, and movable good, diet is normal, does not have dead the generation.Put to death animal, and dissected all no abnormal discoveries such as the perusal heart, liver, spleen, lung, kidney in the 8th day after the administration.It is 0.556g that precious total amount is mended by the prompting mice section of giving birth to, promptly living section benefit treasured is no less than 0.556g to the maximum tolerated dose of the multiple dosing of mice, the adult's taking dose that is converted to 70kg by body surface area is no less than 215.7g, is equivalent to about 216 times of adult's taking dose every day (1g/ day) of 70kg.The living section of prompting benefit treasured is the hypotoxicity sample thus.
Section's benefit is precious to cause the low experimental result of immune function of mice to cyclophosphamide 3.2 give birth to
Give birth to section mend precious to the administration of cyclophosphamide mice during 2 weeks the diving tower experimental result as shown in table 2 below, give birth to section and mend the precious experimental result that mononuclear phagocyte is engulfed chicken red blood cell and be shown in Table 3: give birth to section and mend and precious 4 weeks of administration the exponential experimental result of immune organ is shown in Table 4.
Table 2 give birth to section mend the experimental result of precious diving tower model to the cyclophosphamide mice (
N=10)
| Group | Dosage (g/kg) | Errors number (n) in the 5min | Slip (%) | Behind the 24h | |
| The incubation period (s) of jumping off platform for the first time | Wrong total degree (n) in the 3min | ||||
| The normal control group | NS | 3.2±1.2 ** | --- | 129.5±49.5 *** | 7 |
| The cyclophosphamide model control group | NS | 5.8±1.8 | --- | 49.1±13.9 | 18 |
| Give birth to section and mend precious low dose group | 0.129 | 5.6±2.1 △ | 5.2 | 78.5±41.5 * | 14 |
| Give birth to section and mend dosage group in the treasured | 0.387 | 5.3±1.7 △ | 8.6 | 98.5±47.1 ** | 11 |
| Give birth to section and mend precious high dose group | 1.161 | 4.1±1.1 * | 29.3 | 164.8±27.7 *** | 7 |
| The QINGCHUNBAO PIAN matched group | 1.932 | 4.1±1.5 * | 29.3 | 132.4±57.3 *** | 8 |
Compare with the cyclophosphamide model control group,
*P＜0.05,
*P＜0.01,
* *P＜0.001,
△P＞0.05.
Can get by above experimental result, cyclophosphamide model control group mice school grade (diving tower errors number in the 5min) is compared with the normal control group has significant difference (P＜0.01), its memory achievement (jumping off the incubation period and the interior errors number of 3min of platform for the first time) after 24 hours is compared with normal control group mice has significant difference (P＜0.001), illustrates that learning and memory abilities in aging mice obviously descends.
Give birth to precious each the dosage group of section's benefit and all improve the diving tower school grade of mice to some extent, diving tower mistake slip respectively 5.2%, 8.6%, 29.3% in its 5min, wherein gives birth to section and mends precious high dose group mice school grade and remember achievement and compare with model control group and have significant difference (P＜0.05); The school grade of positive controls mice is compared with model control group has significant difference (P＜0.05), and to mend precious high dose group identical with giving birth to section for its action effect.Behind the 24h, the memory ability of examination mice as seen, the memory achievement of giving birth to the precious basic, normal, high dosage group of section's benefit is to compare with model control group the incubation period of jumping off platform for the first time, has significant difference (P＜0.05, P＜0.01 P＜0.001), has dose-effect relationship preferably between each dosage group.
Prompting is thus given birth to section and is mended precious effect with the learning and memory that promotes mice.
The precious effect to macrophage phagocytosis of mice of the living section of table 3 benefit (
N=10)
| Group | Dosage (g/kg) | Phagocytic rate (%) | Phagocytic index (CRBC number/100 phagocyte) |
| The normal control group | NS | 47.4±14.1 * | 0.569±0.135 * |
| The cyclophosphamide model control group | NS | 34.7±11.7 | 0.406±0.149 |
| Give birth to section and mend precious low dose group | 0.129 | 37.9±13.1 △ | 0.433±0.118 △ |
| Give birth to section and mend dosage group in the treasured | 0.387 | 40.3±13.7 △ | 0.449±0.126 △ |
| Give birth to section and mend precious high dose group | 1.160 | 53.1±9.2 *** | 0.574±0.092 ** |
| The QINGCHUNBAO PIAN matched group | 1.932 | 55.4±10.1 *** | 0.681±0.103 *** |
Compare with model control group,
*P＜0.05,
*P＜0.01,
* *P＜0.001,
△P＞0.05.
Can be got by above experimental result, each organizes the down visible chicken red blood cell clearly of peritoneal fluid smear oil mirror of mice by the phenomenon of macrophage phagocytic.Wherein model control group is because injection cyclophosphamide immunosuppressant, so its phagocytic rate compares obvious decline with phagocytic index with the normal control group, and has significant difference (P＜0.05).Give birth to section's precious basic, normal, high dosage group of benefit and all improve phagocytic rate and phagocytic index to some extent, wherein high dose group is compared with model control group and is had significant difference (P＜0.01, P＜0.001); Positive controls can improve phagocytic rate and phagocytic percentage significantly, compares with model control group to have significant difference (P＜0.001).Therefore give birth to section and mend the activity that precious high dose can improve macrophage, have immunological enhancement preferably.
Table 4 give birth to section mend precious to the exponential experimental result of mouse immune organ (
N=10)
| Group | Dosage (g/kg) | Body weight (g) | Thymus index | Index and spleen index |
| The normal control group | NS | 26.54±2.74 *** | 0.227±0.044 *** | 0.536±0.72 △ |
| The cyclophosphamide model control group | NS | 21.39±2.72 | 0.135±0.029 | 0.451±0.068 |
| Give birth to section and mend precious low dose group | 0.129 | 22.66±2.44 △ | 0.144±0.040 * | 0.463±0.054 △ |
| Give birth to section and mend dosage group in the treasured | 0.387 | 23.65±2.32 △ | 0.174±0.040 * | 0.482±0.077 △ |
| Give birth to section and mend precious high dose group | 1.160 | 24.51±2.45 * | 0.189±0.062 * | 0.625±0.143 ** |
| The QINGCHUNBAO PIAN matched group | 1.932 | 24.21±2.96 * | 0.203±0.043 *** | 0.511±0.070 △ |
Compare with model control group,
*P＜0.05,
*P＜0.01,
* *P＜0.001,
△P＞0.05.
Can be got by above experimental result, cyclophosphamide model group mice body weight obviously reduces, and atrophy all takes place for thymus and spleen, and wherein body weight, thymus index are compared with the normal control group and had significant difference (P＜0.001).Give birth to section and mend body weight, thymus index and the index and spleen index that precious basic, normal, high dosage group can increase mice to some extent, wherein the high dose group These parameters is compared with model control group, has significant difference (P＜0.05, P＜0.01); Positive controls can improve body weight and the thymus index of mice significantly, compares with model control group to have significant difference (P＜0.05, P＜0.001).Therefore give birth to section and mend precious certain enhancing immunity effect that has.
3.3 giving birth to section mends precious to the subacute aging model experimental result of rat due to the D-galactose
Injection of d-galactose is after one week, and the phenomenon of hypotrichosis, tarnish, daily routines minimizing all appears in model group and administration group rat to some extent.Along with the prolongation of injection of d-galactose time, the mental status of rat is relatively poor, and it is slower to take action; Hair is more sparse from head to foot, tarnishes substantially, though diet and take the photograph water and do not see significant change is often completely wet.The learning and memory in rats ability is measured in administration during six weeks water maze laboratory the results are shown in Table shown in 5; Giving birth to precious hemorheology of rat is influenced of section's benefit is shown in Table 6; The changes of biochemical indexes of peripheral blood and cerebral tissue sees 7 respectively, shown in the table 8; The organ index is shown in Table 9.
Table 5 give birth to section mend precious to the D-galactose cause subacute old and feeble rat water maze laboratory result (
N=10)
| Group | Dosage (g/kg) | Seek platform SPL incubation period value (s) | |||
| The N direction | The S direction | The W direction | The E direction | ||
| The normal control group | NS | 13.56±3.39 *** | 7.02±1.53 *** | 7.76±1.81 *** | 9.88±2.77 ** |
| D-galactose model control group | NS | 33.61±8.94 | 29.62±7.39 | 30.47±8.44 | 19.08±3.97 |
| Give birth to section and mend precious low dose group | 0.089 | 28.46±7.80 △ | 25.67±8.70 △ | 23.85±9.74 △ | 13.29±4.40 △ |
| Give birth to section and mend dosage group in the treasured | 0.267 | 22.88±6.09 ** | 21.81±6.07 * | 18.54±6.73 ** | 14.38±2.85 * |
| Give birth to section and mend precious high dose group | 0.804 | 16.42±5.30 *** | 12.56± 6.61 *** | 12.53±2.70 *** | 10.04±2.82 *** |
| The QINGCHUNBAO PIAN matched group | 1.338 | 24.23±6.68 ** | 17.85± 4.59 *** | 11.46±3.30 *** | 15.17±4.65 *** |
| Group | Dosage (g/kg) | Cross over original platform number of times CPT (n) | Cross over other platform number of times (n) | Cross over platform percent CPP value (%) | |
| The normal control group | NS | 4.7±1.2 *** | 8.5±2.1 *** | 36.0±7.9 *** | |
| D-galactose model control group | NS | 2.5±1.0 | 16.4±4.8 | 13.4±4.8 | |
| Give birth to section and mend precious low dose group | 0.089 | 5.5±1.6 *** | 12.3±1.6 * | 30.7±6.9 ** | |
| Give birth to section and mend dosage group in the treasured | 0.267 | 6.2±2.8 *** | 8.8±3.4 *** | 41.7±9.5 ** | |
| Give birth to section and mend precious high dose group | 0.804 | 4.3±1.3 ** | 7.4±1.3 *** | 36.3±7.7 *** | |
| The QINGCHUNBAO PIAN matched group | 1.338 | 7.3±2.6 *** | 9.9±2.3 *** | 41.4±10.8 △ |
Compare with D-galactose model control group,
*P＜0.05,
*P＜0.01,
* *P＜0.001,
△P＞0.05.
Can get by experimental result, the searching platform prolongation of latency of model control group rat, it is crossed over the original platform number of times and crosses over the normal matched group of platform percent and all obviously reduce, compare with the normal control group and to have significant difference (P＜0.001), illustrate that the D-galactose can cause the ability of learning and memory of rat significantly to reduce.That gives birth to N, S that section mends precious each dosage group, W, the entry of E four direction looks for all in various degree shortenings platform incubation period, wherein give birth to section's precious middle and high dose form of benefit and reveal the effect of improving the learning and memory in rats ability preferably, with the same period model control group compare, has significant difference (P＜0.05, P＜0.01, P＜0.001); Also obviously shorten the platform incubation period of looking for of four different directions entry of positive controls, compares with model control group to have significant difference (P＜0.05, P＜0.01, P＜0.001).
The precious hemorheology influence to rat of the living section of table 6 benefit (
N=10)
| Group | Whole blood viscosity (mPas) | ||
| Low shear (l/s) | Middle shear (l/s) | High shear (l/s) | |
| 10.00 | 60.00 | 150.00 | |
| The normal control group | 10.514±1.166 *** | 5.442±0.756 *** | 4.059±0.487 *** |
| D-galactose model control group | 16.195±3.522 | 7.395±1.456 | 6.079±0.988 |
| Give birth to section and mend precious low dose group | 15.639±3.140 △ | 6.952±0.884 △ | 5.719±0.560 △ |
| Give birth to section and mend dosage group in the treasured | 14.790±2.413 △ | 6.51 7±1.186 △ | 4.955±0.756 ** |
| Give birth to section and mend precious high dose group | 13.844±1.516 △ | 6.109±0.672 * | 4.261±1.103 *** |
| The QINGCHUNBAO PIAN matched group | 15.493±2.459 △ | 6.570±0.972 △ | 4.491±0.691 *** |
| Group | Plasma viscosity (mPas) | Serum viscosity in rabbits | Packed cell volume HCT (%) |
| The normal control group | 1.48±0.10 | 1.14±0.05 * | 45.58±6.11 ** |
| D-galactose model control group | 1.64±0.13 | 1.19±0.05 | 56.09±7.63 |
| Give birth to section and mend precious low dose group | 1.59±0.08 △ | 1.16±0.06 △ | 52.66±3.74 △ |
| Give birth to section and mend dosage group in the treasured | 1.55±0.08 △ | 1.15±0.07 △ | 51.36±4.05 △ |
| Give birth to section and mend precious high dose group | 1.54±0.08 △ | 1.15±0.06 △ | 49.23±5.78 * |
| The QINGCHUNBAO PIAN matched group | 1.56±0.06 △ | 1.16±0.04 | 50.14±5.64 △ |
| Group | Fibrin (mPas) viscosity | Platelet aggregation rate (%) | |
| The normal control group | 0.34±0.12 △ | 42.86±3.01 *** | |
| D-galactose model control group | 0.45±0.15 | 57.04±6.83 | |
| Give birth to section and mend precious low dose group | 0.43±0.11 △ | 54.14±4.76 △ | |
| Give birth to section and mend dosage group in the treasured | 0.40±0.13 △ | 53.06±3.80 △ | |
| Give birth to section and mend precious high dose group | 0.39±0.10 △ | 50.58±3.60 * | |
| The QINGCHUNBAO PIAN matched group | 0.40±0.09 △ | 52.08±3.40 △ |
Compare with model control group,
*P＜0.05,
*P＜0.01,
* *P＜0.001,
△P＞0.05.
Can get by above experimental result, the basic, normal, high shear of model control group rat whole blood viscosity, serum viscosity in rabbits plasma viscosity, packed cell volume and platelet aggregation rate all obviously raise, and compare with the normal control group, has significant difference (P＜0.05, P＜0.01, P＜0.001), prompting D-galactose causes that subacute old and feeble disease due to the body carbohydrate metabolism disturbance of rat is learned the blood flow limit and exerts an influence, it becomes sticky the blood of rat, easily coagulates.Giving birth to section mends each precious dosage group the blood viscosity of rat is had in various degree reduction, but the middle and high shear value of the whole blood viscosity of having only high dose group, packed cell volume is compared with model control group with platelet aggregation rate, has significant difference (P＜0.05, P＜0.001), tentatively point out it to have certain function of promoting blood circulation to disperse blood clots.The high shear value of the whole blood viscosity of positive controls rat with the same period model control group compare, have significant difference (P＜0.001), it improves a little less than the effect of hemorheology subacute old and feeble rat.
Table 7 give birth to section mend precious to the D-galactose cause the serum biochemistry index of subacute old and feeble rat influence (
N=10)
| Group | Dosage (g/kg) | MDA (nmol/ml) | SOD (NU/ml) | MAO (U/h/ml) | GSH-Px (U) |
| The normal control group | NS | 3.21±0.90 *** | 298.98±40.09 *** | 11.37±2.50 | 228.30± 49.16 *** |
| D-galactose model control group | NS | 7.07±1.74 | 165.35±56.71 △ | 22.83±4.06 | 138.43± 26.08 |
| Give birth to section and mend precious low dose group | 0.089 | 5.55±1.38 * | 183.44±58.71 △ | 18.18±3.97 * | 159.54± 57.31 △ |
| Give birth to section and mend dosage group in the treasured | 0.268 | 4.90±1.41 ** | 202.31±71.21 △ | 16.47±3.75 ** | 183.97± 56.26 * |
| Give birth to section and mend precious high dose group | 0.804 | 4.31±1.04 *** | 240.36±56.88 ** | 15.42±5.03 ** | 194.67± 53.58 ** |
| The QINGCHUNBAO PIAN matched group | 1.338 | 4.66±0.70 *** | 228.45±54.94 | 16.18±3.33 *** | 176.13± 47.65 * |
Compare with model control group,
*P＜0.05,
*P＜0.01,
* *P＜0.001,
△P＞0.05.
Experimental result by table 7 can get, and the content of MDA, MAO increases in the model control group rat blood serum, and the content of SOD and GSH-Px then reduces, and the content of the rat interior free yl of prompting injection of d-galactose improves, and the oxidation resistance of body reduces.Give birth to section and mend the content that MDA in the rat blood serum, MAO all can fall in each precious dosage group to some extent, the content of SOD and GSH-Px in the rising serum: wherein give birth to section and mend the content of MDA in the precious basic, normal, high dosage group rat blood serum and compare (P＜0.05 that has significant difference with model control group, P＜0.01), has dose-effect relationship preferably between three dosage groups; SOD value in the high dose group rat blood serum of living section benefit treasured is compared with model control group has significant difference (P＜0.01); The content that MAO in the precious basic, normal, high dosage group rat blood serum mends in living section is compared with model control group has significant difference (P＜0.05, P＜0.01); The content that GSH-Px in the precious middle and high dosage group rat blood serum mends in living section is compared with model control group has significant difference (P＜0.05, P＜0.01).The action effect of positive controls is close with the effect of giving birth to dosage group in section's benefit treasured.Prompting is thus given birth to section and is mended the precious generation that can remove the peroxide radical of body preferably, and the protection cell reduces the damage of free radical, improves the aging action that it has the antagonism body.
The influence of the cerebral tissue mesophytization index of the precious subacute old and feeble rat of table 8 living section benefit (
N=10)
| Group | Dosage (g/kg) | MDA (nmol/mgprot) | SOD (NU/mgprot) | MAO (U/h/mgprot) | GSH-Px (U) |
| The normal control group | NS | 4.40±0.56 *** | 73.25± 19.83 *** | 6.99±0.73 *** | 27.80±1.20 *** |
| D-galactose model control group | NS | 8.44±2.66 | 39.65± 18.13 | 13.7±1.67 | 13.90±3.06 |
| Give birth to section and mend precious low dose group | 0.129 | 7.23±1.51 △ | 46.26± 17.15 △ | 11.23±1.78 ** | 16.03±5.44 △ |
| Give birth to section and mend dosage group in the treasured | 0.387 | 6.44±1.43 △ | 50.51± 17.51 △ | 9.01±1.26 *** | 19.53±4.53 *** |
| Give birth to section and mend precious high dose group | 1.160 | 5.77±0.94 ** | 53.66± 18.29 △ | 8.50±2.85 *** | 22.28±2.77 *** |
| The QINGCHUNBAO PIAN matched group | 1.932 | 6.26±1.30 ** | 47.11± 16.33 △ | 9.88±1.42 *** | 20.75±1.43 *** |
Compare with model control group,
*P＜0.05,
*P＜0.01,
* *P＜0.001,
△P＞0.05.
Can get by above experimental result, MDA in the model control group rat brain tissue and the content of MAO increase, the content of SOD and GSH-Px descends, compare (P＜0.01 that has significant difference with the normal control group, P＜0.001), with because long term injections D-galactose causes the general metabolism disorder so that the decline of each organ, systemic-function causes that free radical is piled up, cellulose is impaired causes old and feeble theory and be consistent.Living section mends precious high dose and can reduce MDA content in the subacute old and feeble rat cerebral tissue, compares with model control group to have significance poor (P＜0.05); Give birth to section and mend precious basic, normal, high dosage group and can reduce the content of the SOD enzyme in the subacute old and feeble rat cerebral tissue and have dose-effect, do not have significant difference (P＜0.05) but compare with model control group; Give birth to section and mend can the raise content of the SOD enzyme in the subacute old and feeble rat cerebral tissue of precious basic, normal, high dosage group, compare with model control group and have significant difference (P＜0.01, P＜0.001); Give birth to section and mend the GSH-Px content of precious middle and high dosage group in can subacute old and feeble rat cerebral tissue, compare with model control group and have significant difference (P＜0.01, P＜0.001).More than explanation is given birth to section's benefit treasured and can be removed too much free radical in the body, thereby helps protecting cell membrane stability to reach antidotal pharmacological action.Can the raise content of the GSH-Px in the subacute old and feeble rat cerebral tissue of positive drug QINGCHUNBAO PIAN reduces the content of MDA and MAO, compares with model control group to have significant difference (P＜0.05, P＜0.001); Though the SOD activity in the positive controls rising rat cerebral tissue is compared with model control group and not to be had significant difference.Precious administration group is mended by living section and the SOD of positive controls rat cerebral tissue value individual variation is bigger, therefore influences the t assay
The precious influence to rat body weight and organ index of the living section of table 9 benefit (
N=10)
| Group | Dosage (g/kg) | Body weight (g) | Thymus index (/ 100g | Index and spleen index (/ 100g) | Cerebral index (/ 100g) |
| The normal control group | NS | 361.5±25.5 △△ | 0.085± 0.023 *** | 0.328± 0.051 | 0.578± 0.056 |
| D-galactose model control group | NS | 355.5±44.6 | 0.041±0.009 | 0.267± 0.054 | 0.533± 0.080 |
| Give birth to section and mend precious low dose group | 0.129 | 367.5±18.9 △ | 0.050±0.010 * | 0.278± 0.063 | 0.534± 0.042 |
| Give birth to section and mend dosage group in the treasured | 0.387 | 370.5±35.4 △ | 0.054±0.007 ** | 0.282± 0.054 | 0.544± 0.064 |
| Give birth to section and mend precious high dose group | 1.160 | 402.8±40.5 * | 0.081± 0.025 *** | 0.302± 0.059 | 0.539± 0.064 |
| The QINGCHUNBAO PIAN matched group | 1.932 | 388.6±45.0 △ | 0.061±0.016 ** | 0.292± 0.067 | 0.509± 0.058 |
Compare with model control group,
*P＜0.05,
*P＜0.01,
* *P＜0.001,
△P＞0.05.
Can get by above experimental result,, compare there was no significant difference (P＞0.05) with the normal control group though the body weight of model control group rat slightly descends; Atrophy all takes place in its thymus, spleen, and thymus index is compared with model control group with index and spleen index, has significant difference (P＜0.05, P＜0.001); The full brain of model control group is not seen obvious atrophy in addition, compares there was no significant difference (P＞0.05) with the normal control group.Give birth to section and mend all slightly increases of body weight of three precious dosage groups, wherein the high dose group rat body weight is compared with model control group, has significant difference (P＜0.05); Give birth to the thymus index of the precious middle and high dosage group rat of section's benefit and compare, have significant difference (P＜0.01, P＜0.001) with model control group; Mend precious index and spleen index increase though give birth to section, compare there was no significant difference (P＞0.05) with model control group; Give birth to section's each precious dosage group rat whole brain index of benefit and compare difference that there are no significant (P＞0.05) with model control group.The thymus index of positive controls rat is compared with model control group, has significant difference (P＞0.05).All the other body weight, index and spleen index and cerebral index are compared with model control group, there was no significant difference (P＞0.05).Prompting is thus given birth to section and is mended precious effect with atrophy of immune organ that certain alleviation aging causes.
Mend the precious experimental result that the D-galactose is caused the subacute aging of mice 3.4 give birth to section
After 4 weeks of continuous subcutaneous injection D-galactose, except that the normal control group, each organizes phenomenons such as the visible hair of mice naked eyes is matt and sparse, comparatively slow, the spiritual atrophy of action is depressed.Influence to the autonomic activities of mice is shown in Table 10; Influence to the experiment of mice diving tower is shown in Table 11; Influence to mice body weight and immune organ is shown in Table 12.
Table 10 give birth to section mend the influence of precious autonomic activities to subacute aging model mice (
N=10)
| Group | Dosage (g/kg) | 5min autonomic activities number of times (n) | Improvement rate (%) |
| The normal control group | NS | 226±26 *** | --- |
| D-galactose model control group | NS | 142±42 | --- |
| Give birth to section and mend precious low dose group | 0.129 | 152±28 △ | 5.3 |
| Give birth to section and mend dosage group in the treasured | 0.387 | 164±30 △ | 13.9 |
| Give birth to section and mend precious high dose group | 1.160 | 165±22 △ | 14.6 |
| The QINGCHUNBAO PIAN matched group | 1.932 | 172±28 △ | 19.4 |
Compare with model control group,
* *P＜0.001,
△P＞0.05.
Can be got by experimental result, the autonomic activities amount of model control group mice is compared remarkable reduction (P＜0.001) with the normal control group, and prompting is adopted the D-galactose to cause the subacute old and feeble feature of mice and showed; The autonomic activities improvement rate of giving birth to precious basic, normal, high each the dosage group mice of section's benefit is respectively 5.3%, 13.9%, 14.6%, and mice autonomic activities number of times is compared significant difference (P＞0.05) in the 5min with model control group; Positive drug QINGCHUNBAO PIAN control group mice autonomic activities improvement rate is 19.4%, and the autonomic activities number of times is compared there was no significant difference (P＞0.05) with model control group; Mend the treasured tool and have some improvement though this shows living section, its improvement effect is not remarkable.
Table 11 give birth to section mend the effect of precious diving tower model to subacute aging model mice (
N=10)
| Group | Dosage (g/kg) | Errors number (n) in the 5min | Slip (%) | Behind the 24h | |
| The incubation period (s) of jumping off platform for the first time | Wrong total degree (n) in the 3min | ||||
| The normal control group | NS | 4.4±1.6 *** | --- | 116.4±37.7 *** | 6 |
| D-galactose model control group | NS | 11.6±3.9 | --- | 50.2±15.0 | 24 |
| Give birth to section and mend precious low dose group | 0.129 | 8.9±2.5 △ | 23.3 | 65.7±17.6 * | 20 |
| Give birth to section and mend dosage group in the treasured | 0.387 | 5.8±2.7 *** | 50.0 | 78.6±33.6 * | 13 |
| Give birth to section and mend precious high dose group | 1.160 | 5.3±2.8 *** | 54.3 | 92.8±35.5 ** | 9 |
| The QINGCHUNBAO PIAN matched group | 1.932 | 7.1±2.4 ** | 38.8 | 83.7±38.6 * | 12 |
Compare with model control group,
*P＜0.05,
*P＜0.01,
* *P＜0.001,
△P＞0.05.
Can get by above experimental result, D-galactose model control group mice school grade (diving tower errors number in the 5min) is compared with the normal control group has significant difference (P＜0.001), its memory achievement (jumping off the incubation period and the interior errors number of 3min of platform for the first time) after 24 hours is compared with normal control group mice has significant difference (P＜0.001), illustrates that the learning capacity of subacute mouse aging obviously descends.Give birth to precious each the dosage group of section's benefit and all improve the diving tower school grade of mice to some extent, diving tower mistake slip difference 23.3%, 50.0%, 54.3% in its 5min, wherein giving birth to section mends precious middle and high dosage group mice school grade and remembers achievement and compare (P＜0.05 that has significant difference with model control group, P＜0.01, P＜0.001); The school grade of positive controls mice is compared with model control group has significant difference (P＜0.05, P＜0.01).Prompting is thus given birth to section and is mended precious high dose and have and promote learning and memory abilities in aging mice significantly.
Table 12 give birth to section mend precious to subacute aging model mice body weight and the exponential influence of immune organ (
N=10)
| Group | Dosage (g/kg) | Body weight (g) | Thymus index (%) | Index and spleen index (%) |
| The normal control group | NS | 28.24±2.51 *** | 0.166±0.034 *** | 0.992±0.112 *** |
| D-galactose model control group | NS | 23.45±2.08 | 0.094±0.020 | 0.639±0.124 |
| Give birth to section and mend precious low dose group | 0.129 | 25.95±1.90 * | 0.104±0.031 ** | 0.709±0.195 ** |
| Give birth to section and mend dosage group in the treasured | 0.387 | 25.96±2.15 * | 0.114±0.033 △ | 0.831±0.227 △ |
| Give birth to section and mend precious high dose group | 1.160 | 25.29±2.08 * | 0.142±0.003322 △ | 0.881±0.179 * |
| The QINGCHUNBAO PIAN matched group | 1.932 | 25.54±1.96 △ | 0.139±0.043 *** | 0.820±0.153 ** |
Compare with model control group,
*P＜0.05,
*P＜0.01,
* *P＜0.001,
△P＞0.05.
Can get by experimental result, long term injections D-galactose makes the body weight gain of respectively organizing mice slow, model control group mice body weight is compared with the normal control group has significant difference (P＜0.001), atrophy all takes place in its thymus and spleen, compare with the normal control group and to have significant difference (P＜0.001), point out subacute aging model to prepare successfully.Give birth to section and mend precious increase along with dosage, the weight increase of mice is slow more, compares with model control group to have significant difference (P＜0.01); Give birth to section and mend the thymus of precious each dosage group mice and all generations atrophy of spleen, but increase along with administration concentration, its degree is eased, and the thymus index that precious high dose group mice is mended by wherein living section is compared with model control group with index and spleen index has significant difference (P＜0.01, P＜0.001); Difference (P＞0.05) that all the other each indexes compare with model control group that there are no significant.Positive controls can increase the body weight of subacute mouse aging, but compares there was no significant difference (P＞0.05) with model control group, and QINGCHUNBAO PIAN can be improved the atrophy of thymus and spleen preferably, compares with model control group to have significant difference (P＜0.01).
4. experiment conclusion
Comprehensive above-mentioned experimental result can get, and gives birth to section and mends and preciously once reaches repeatedly oral administration on the one through mice, and its maximum tolerated dose is equivalent to 86 times and 216 times of adult's taking dose every day (1g/ day) of 70kg, and toxic reaction all do not occur; Give birth to section and mend precious high dose group and show immunological enhancement preferably, significantly improve the phagocytic rate and the phagocytic index of mouse macrophage, improve mouse immune organ index, the tool learning and memory abilities in aging mice that improves; Give birth to section and mend precious middle and high dosage subacute old and feeble rat and mice due to the D-galactose; all have and improve memory ability significantly, remove the generation of intravital peroxide radical (MDA, MAO) preferably; improve the content of anti-oxidation active substance (SOD, GSH-Px) in the body, help protecting cell membrane stability.In above experiment, the dosage (1.161g/kg) that precious high dose group is mended by living section is lower than the dosage (1.932g/kg) of positive controls QINGCHUNBAO PIAN and the dosage (3.132g/kg) of positive control drug SHENQI JINGWU JIAONANG, shows that giving birth to section's benefit treasured has pharmacologically active preferably.
Claims (7)
1, a kind of Chinese medicinal capsule that is used to regulate immunity is characterized in that this capsule is to be made by the raw material of following weight ratio:
Coriolous Dersicolor (Fr.) Quel powder 4-16 part after the extraction
Lentinus Edodes powder 3-12 part after the extraction
Astragalus membranaceus powder 3-12 part after the extraction.
2,, it is characterized in that this capsule is to be made by the raw material of following weight ratio according to the Chinese medicinal capsule of claim 1:
12 parts in Coriolous Dersicolor (Fr.) Quel powder after the extraction
9 parts in Lentinus Edodes powder after the extraction
9 parts of astragalus membranaceus powders after the extraction.
3, a kind of preparation method of Chinese medicinal capsule as claimed in claim 1 is characterized in that this method may further comprise the steps:
A. take by weighing respectively and extract back Coriolous Dersicolor (Fr.) Quel powder, extract back Lentinus Edodes powder, it is stand-by that extraction back astragalus membranaceus powder is crossed 60 mesh sieves; All raw materials carry out outward appearance and detect, and place and mix powder in the mixer;
B. collect the mixed powder of step a, with double-layer polyethylene poly-bag packed for standby use through mix homogeneously;
C. the mixed powder that step b is collected carries out capsule charge in automatic or semi-automatic capsule filler;
D. capsule through polishing, check, go into bottle, go into the box packing and promptly get product.
4, preparation method according to claim 3, the preparation method of the Coriolous Dersicolor (Fr.) Quel powder after it is characterized in that extracting is: get qualified Coriolous Dersicolor (Fr.) Quel, clean, the removal of impurity, clear water is cleaned, chopping, put in the multi-function extractor, add 8-12 times of water gaging, boil, extract 2-4 time, boil extraction time is 1.5-2.5 hour at every turn, extracting solution 200 orders are crossed screen cloth, get filtrate ,-80--95kpa, 65-80 ℃ of decompression, concentrate, 60 ℃ of relative densities are that 1.08-1.10 crosses 120 orders, merge concentrated solution, be chilled to room temperature, stir the ethanol of slow adding 95% down, just the alcohol amount 85% that contains of concentrated solution clear paste stirs and left standstill 24 hours, filter, get the precipitation drying under reduced pressure, be ground into smalls, promptly make the Coriolous Dersicolor (Fr.) Quel powder after the extraction.
5, preparation method according to claim 3, the preparation method of the Coriolous Dersicolor (Fr.) Quel powder after it is characterized in that extracting is: get qualified Coriolous Dersicolor (Fr.) Quel, clean, the removal of impurity, clear water is cleaned, chopping, put in the multi-function extractor, add 10 times of water gagings, boil, extract 3 times, boil extraction time to be 2 hours at every turn, extracting solution 200 orders are crossed screen cloth, get filtrate ,-90kpa, 75 ℃ of decompressions, concentrating, is that 1.08-1.10 crosses 120 orders 60 ℃ of following relative densities, merges concentrated solution, be chilled to room temperature, stir the ethanol of slow adding 95% down, make the alcohol amount 85% that contains of concentrated solution clear paste, stir and left standstill 24 hours, filter, get the precipitation drying under reduced pressure, be ground into smalls, promptly make the Coriolous Dersicolor (Fr.) Quel powder after the extraction.
6, preparation method according to claim 3, the Lentinus Edodes powder after it is characterized in that extracting and the preparation method of astragalus membranaceus powder are: get qualified Lentinus Edodes respectively, the Radix Astragali cleans, the removal of impurity, clear water is cleaned, chopping is put in the multi-function extractor, adds 8-12 times of water gaging, boil, extract 2-4 time, boil extraction time to be 0.5-1.5 hour at every turn, extracting solution 200 orders are crossed screen cloth, get filtrate,-80--95kpa, 65-80 ℃ of decompression concentrates, 60 ℃ of relative densities are that 1.05-1.08 crosses 120 orders, merge concentrated solution, spray drying makes the Lentinus Edodes powder after the extraction respectively, astragalus membranaceus powder after the extraction.
7, preparation method according to claim 3, the Lentinus Edodes powder after it is characterized in that extracting and the preparation method of astragalus membranaceus powder are: get qualified Lentinus Edodes, the Radix Astragali respectively, clean, the removal of impurity, clear water is cleaned, chopping is put in the multi-function extractor, adds 10 times of water gagings, boil, extract 3 times, boil extraction time to be 1 hour at every turn, extracting solution 200 orders are crossed screen cloth, get filtrate,-90kpa, 75 ℃ of decompressions concentrate, 60 ℃ of following relative densities is that 1.05-1.08 crosses 120 orders, merge concentrated solution, spray drying makes Lentinus Edodes powder after the extraction respectively, the astragalus membranaceus powder after extracting.
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| CN103704704B (en) * | 2013-12-31 | 2016-01-27 | 陈慧婷 | A kind of dogwood fruit dispersing tablet and preparation method |
| CN103652948B (en) * | 2013-12-31 | 2015-06-17 | 陈慧婷 | Chinese yam and astragalus membranaceus dispersible tablets and preparation method thereof |
| CN103704710B (en) * | 2013-12-31 | 2016-01-27 | 陈慧婷 | A kind of Chinese yam pericarpium citri reticulatae viride dispersible tablet and preparation method |
| CN103750305B (en) * | 2013-12-31 | 2015-06-10 | 陈慧婷 | Chinese yam and astragalus membranaceus dispersible tablet and preparation method thereof |
| CN105726865A (en) * | 2016-02-25 | 2016-07-06 | 毛德英 | Lentinan oral liquid and preparation method and application thereof |
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| CN1056313C (en) * | 1994-11-10 | 2000-09-13 | 天津市农业生物工程研究中心 | Functional oral liquor for enhancing human immunity |
| CN1583159A (en) * | 2003-08-19 | 2005-02-23 | 初真文 | Health care products of lucid ganoderma and their preparation |
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| CN1056313C (en) * | 1994-11-10 | 2000-09-13 | 天津市农业生物工程研究中心 | Functional oral liquor for enhancing human immunity |
| CN1583159A (en) * | 2003-08-19 | 2005-02-23 | 初真文 | Health care products of lucid ganoderma and their preparation |
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