CN1957967A - Application of pseudostellaria root for preparing medication of treating cardiovascular disease - Google Patents
Application of pseudostellaria root for preparing medication of treating cardiovascular disease Download PDFInfo
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Abstract
An application of pseudostellaria root in preparing the medicines for treating coronary heart disease, angina pectoris, myocardial ischemia and chronic cardiac functional insufficiency is disclosed.
Description
Technical field
The present invention relates to the application in cardiovascular disease medicines such as preparation treatment coronary heart disease, angina pectoris, myocardial ischemia, chronic cardiac insufficiency of the novel clinical use of Radix Pseudostellariae, particularly Radix Pseudostellariae extract, belong to technical field of medicaments.
Background technology
Radix Pseudostellariae is the dried root of Caryophyllaceae Pseudostellaria Pax plant Radix Ginseng Pseu-dostellaria heterophylla (Miq.) Pax exPax et Hoffm, and elongated spindle of root or elongated strip shaped are crooked slightly, long 3～10cm, diameter 0.2～0.6cm.There is the stem trace on the top, and surperficial yellow-white is more smooth, and little have a vertical wrinkle, and recess has mark of fibrous root.Matter is hard and crisp, and section is smooth, yellowish white.The property flat, sweet in the mouth, little hardship, include fructose, starch (35.1%), saponin, several amino acids, vitamin etc., and equal tool physiologically actives.Radix Pseudostellariae has the effect of supplementing QI for promoting the production of body fluid, tonifying the lung spleen invigorating, is mainly used in insufficiency of the spleen lack of appetite, fatigue and weakness, cough due to deficiency of the lung, insomnia, body fluid deficiency xerostomia and disease such as QIYINLIANGXU after being ill at present, is the product of well clear benefit simply.It both can with the other drug compatibility, again can single fry in shallow oil the water temperature clothes, common dose is 10～30 grams, can add and subtract in right amount according to the state of an illness.
Summary of the invention
The objective of the invention is to, the application of Radix Pseudostellariae in preparation treatment cardiovascular disease medicine is provided.With Radix Pseudostellariae is that the pharmaceutical preparation that raw material is made has significant therapeutical effect to cardiovascular disease such as coronary heart disease, angina pectoris, myocardial ischemia, chronic cardiac insufficiencies.
Technical scheme of the present invention is that Radix Pseudostellariae is used in the preparation treatment cardiovascular disease medicine.Radix Pseudostellariae extract is applied to prepare the medicine for the treatment of cardiovascular disease, can adopt any dosage form that allows clinically.Can be medicinal preparation for oral administration, as powder, tablet, granule, capsule, microcapsule, pill, drop pill, soft capsule, oral liquid, syrup, dispersible tablet, spray or aerosol etc.; Also can be non-medicinal preparation for oral administration, as suppository, injection (comprising intravenous injection, intramuscular injection), ointment, inhalant, gel etc.
The present invention is used to prepare the medicine for the treatment of cardiovascular disease with Radix Pseudostellariae, with the Radix Pseudostellariae is raw material, technology obtains the Radix Pseudostellariae total extract through extraction routinely, and then add corresponding adjuvant, be prepared into any oral or non-peroral dosage form that is suitable for using clinically, as powder, tablet, granule, capsule, microcapsule, pill, drop pill, soft capsule, oral liquid, syrup, dispersible tablet, spray or aerosol, suppository, injection (comprising intravenous injection, intramuscular injection), ointment, inhalant, gel etc.Used adjuvant comprises excipient substances such as conventional solvent, disintegrating agent, correctives, antiseptic, coloring agent, binding agent, lubricant, wetting agent, thickening agent, solubilizing agent, substrate.
The present invention is used to prepare the medicine for the treatment of cardiovascular disease with Radix Pseudostellariae, can also form compound medicine with one or more the medicine in wind-cold-dispersing medicinal, wind-heat-dispersing medicinal, drugs for dispelling internal cold, QI regulating medicine, stasis-resolving hemostatic, drug for invigorating blood circulation and eliminating stasis, awaking drug, the qi-restoratives medicine.
The application of above-mentioned Radix Pseudostellariae in preparation treatment cardiovascular disease medicine, described wind-cold-dispersing medicinal is Ramulus Cinnamomi, Herba Asari or Rhizoma Zingiberis Recens; Described wind-heat-dispersing medicinal is Herba Menthae, Radix Puerariae or Rhizoma Cimicifugae; Described drugs for dispelling internal cold is Radix Aconiti Lateralis Preparata, Rhizoma Zingiberis, Cortex Cinnamomi, Fructus Evodiae, Pericarpium Zanthoxyli or Flos Caryophylli; Described QI regulating medicine is Pericarpium Citri Reticulatae, Pericarpium Citri Reticulatae Viride, the Radix Aucklandiae, Rhizoma Cyperi, Fructus Toosendan or the Radix Linderae; Described stasis-resolving hemostatic is Radix Notoginseng, Pollen Typhae or Radix Rubiae; Described drug for invigorating blood circulation and eliminating stasis is Rhizoma Chuanxiong, Rhizoma Corydalis, Radix Curcumae, Rhizoma Curcumae Longae, Olibanum, Myrrha, Radix Salviae Miltiorrhizae, Flos Carthami, Semen Persicae, Herba Leonuri, Radix Achyranthis Bidentatae, Radix Ilicis Pubescentis, Rhizoma Curcumae, Hirudo, Sanguis Draxonis or Semen Strychni; Described awaking drug is Moschus, Borneolum Syntheticum or Styrax; Described qi-restoratives medicine is Radix Ginseng, Radix Panacis Quinquefolii, Radix Codonopsis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Rhizoma Dioscoreae, Radix Glycyrrhizae or Radix Et Caulis Acanthopanacis Senticosi.
The present invention is raw material with the Radix Pseudostellariae, technology obtains the extract of Radix Pseudostellariae through extraction routinely, add adjuvant again and make the conventional oral or non-peroral dosage form that is suitable for using clinically, be used for the treatment of cardiovascular disease such as coronary heart disease, angina pectoris, myocardial ischemia and chronic cardiac insufficiency, tangible curative effect is arranged.
The present invention uses the medicine of Radix Pseudostellariae preparation treatment cardiovascular disease, its curative effect of medication can by Radix Pseudostellariae extract to rat acute myocardial infarction animal model influence experiment and Radix Pseudostellariae extract is induced the influence of experimental myocardial hypertrophy to test to the rat coronary ligation and is showed protective effect experiment, the Radix Pseudostellariae of isolated rabbit heart ischemical reperfusion injury.
Below be experimental example of the present invention:
Experimental example 1: Radix Pseudostellariae extract is to the influence of rat acute myocardial infarction animal model
1 experiment material and method
1.1 material and animal grouping
1.1.1 experimental apparatus and reagent
Radix Pseudostellariae extract, this research department's independent development is chocolate brown powder, people's consumption is 1～12g (extract)/10～120g crude drug/60kg.
Animal respirator (HX-300 Chengdu Tai Ming Science and Technology Ltd.)
Electrocardiograph (medical electronic apparatus factory of ECG-11D Ke Meisi group)
High speed electric centrifuge (the sub-company limited of Jintan, YXJ-2A Jiangsu hundred million energisings)
TDL-4ZB table-type low-speed autobalance centrifuge (Hunan Xingke Scientific Instrument Co., Ltd.)
Portable High pressure steam sterilizer (the bright forever Medical Instruments in model GMSX-280 Beijing factory)
Electronic balance (numbering: 04005955 model: Tianjin, island IBLZ20H)
Malonaldehyde (MDA) test kit (bio-engineering research institute is built up in Nanjing)
Superoxide dismutase (SOD) test kit (bio-engineering research institute is built up in Nanjing)
Total antioxidant capacity (T-AOC) test kit (bio-engineering research institute is built up in Nanjing)
1.1.2 laboratory animal
The healthy SD rat, male and female half and half, body weight 200～250g; Guiyang Medical College experimental animal center, quality certification SCXK (Guizhou Province) 2002-2001.Animal is raised with test full-valence pellet feed (Guiyang Medical College experimental animal center provides), freely drinks water 22 ± 2 ℃ of room temperatures.
1.2 Animal Model Making
1.2.1 rat acute myocardial infarction model
Get 50 of SD rats, male and female half and half, body weight 200～250g is divided into 5 groups at random, that is: normal group (giving the isometric(al) normal saline), model group (giving the isometric(al) normal saline), drug component are three dosage group: 2.5g/kg, 5g/kg, 10g/kg, and the administration volume is 10ml/kg; The 20min intraperitoneal injection is 1 time before each group and the operation; 60min is administration 1 time after the ligation.In rat breastbone left side, the parallel vertical opening that is about 2cm with breastbone cuts skin, and passivity is separated pectoralis major, exposes rib.With cotton thread skin and muscle are done purse string suture, make a call to a untwisting.Put respirator, insert between the 5th, 6 rib at distance breastbone 2mm place with mosquito forceps, enlarge otch, carefully with the broken pericardium of tweezers, extruding abdominal part, expose heart in external, with the coronary artery under the atraumatic suture ligaturing left atrial appendage, with heart careful put back to the thoracic cavity, the extruding air, take out tight standby untwisting rapidly, close the thoracic cavity, treat that animal recovers autonomous respiration after, close respirator.Observe Electrocardiographic change, and can determine whether that ligation arrives coronary artery, with the high standard of lifting 0.1mv of J point as the model success by Electrocardiographic change; After 6h had remembered electrocardiogram, the common carotid artery intubate was got blood, and serum is used to detect lactic acid dehydrogenase (LDH), and creatine kinase (CK) is observed the change of biochemical indicator.Operation back 6h has write down ECG, cuts off the thoracic cavity, takes out heart, is put in the plate that is placed with phosphate buffer in advance, squeezes the residue blood that goes in the heart, cuts off the fatty tissue of adhering to muscle, and cuts off the atrium, only stays the ventricle part.With absorbent paper liquid is blotted, with scalpel heart is cut into the 5-6 sheet then, weigh, as heart gross weight before the dyeing, be put among the 1%TTC, take out behind 37 ℃ of water-bath dyeing 5min, blot water, painted normal myocardium (redness) and the infarcted region that is not colored are cut, and infarcted region is weighed, calculating myocardium infarct size percentage rate.
Myocardial infarction area %=weight * 100% of myocardium weight/whole-heartedly that is unstained.
1.3 data analysis and statistical method
All data represent that with x ± s statistical analysis adopts the student-T check.
2. result
2.1 Radix Pseudostellariae extract is to the influence of rat acute myocardial infarction model
After model group ligation 20min minute, the displacement of J point significantly increases, and obvious increase is not seen in the displacement of three dosage group electrocardiogram J points behind the Radix Pseudostellariae drug administration by injection, and is remarkable with the model group comparing difference.The results suggest that infarct size is measured, high behind the Radix Pseudostellariae drug administration by injection, in 2 dosage groups obviously reduce the infarction rate of acute myocardial infarction, with model group comparing difference remarkable (p＜0.05), high, in 2 dosage groups obviously reduce the vigor of CK in the serum, three dosage groups all significantly reduce the vigor of LDH in the serum.Experimental result see Table 1 with table 2.This experimental result prompting Radix Pseudostellariae extract has the certain protection effect to the myocardial ischemia animal model.
Table 1 Radix Pseudostellariae extract is to the infarction fraction values influence of myocardial infarction due to the rat coronary ligation
(x±s)(n＝10)
| Group | Dosage g/kg | Heavy (g) whole-heartedly | Ventricle heavy (g) | Infarcted myocardium heavy (g) | Infarcted region/(%) whole-heartedly | Infarcted region/ventricle (%) |
| Sham-operation group model group Radix Pseudostellariae extract | - 10 5 2.5 | 0.83 ±0.15 0.88 ±0.17 0.87 ±0.16 0.84 ±0.15 0.84 ±0.20 | 0.78 ±0.14 0.83 ±0.15 0.81 ±0.16 0.80 ±0.14 0.80 ±0.19 | 0.00 ±0.00 0.20 ±0.05 0.11 ±0.02 0.15 ±0.02 0.14 ±0.04 | 0.00 ±0.00 22.88 ±4.84 ## 12.98 ±1.88 ## 17.81 ±3.00 * 17.56 ±5.17 * | 0.00 ±0.00 24.08 ±5.03 ## 14.02 ±1.88 ## 19.16 ±3.27 * 18.49 ±5.49 * |
Compare with sham operated rats:
##P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01;
Table 2 Radix Pseudostellariae extract is to the influence of myocardial ischemia CK, LDH due to the coronary ligation
| Group | Dosage (mg/kg) | CK | LDH |
| Sham operated rats model group Radix Pseudostellariae extract | -- -- 10 5 2.5 | 435.71±20.20 483.33±39.29 # 436.42±26.77* 439.28±31.13* 446.78±65.93 | 265.4±44.9 354.2±6.8# 275.26±20.20** 299.82±12.01* 299.58±19.6** |
Compare with model group
#P＜0.05,
##P＜0.01;
Compare * P＜0.05, * * P＜0.01 with the normal control group.
Experimental example 2: Radix Pseudostellariae extract is to the protective effect of isolated rabbit heart ischemical reperfusion injury
1. experiment material
1.1 medicine and reagent lactic acid dehydrogenase (LDH-L) test kit, creatine kinase (CK) test kit, Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd..
1.2 instrument TG328A photoelectric analytical balance, last Nereid section; 721 spectrophotometers; HL-2 type constant flow pump, west, Shanghai, Qingpu, Shanghai instrument plant; Chengdu Tai Meng scientific ﹠ technical corporation of BL-420E system.
1.3 animal
New zealand rabbit, male and female half and half, body weight 2.0-2.4kg provides by the Guiyang Medical College Experimental Animal Center, the animal quality certification number: SCXK (Guizhou Province) 2002-0001.
1.4 statistical method: all data adopt the student-t check; P＜0.05 is for difference has significance, and P＜0.01 difference has utmost point significance.
2. experimental technique and result
2.1 the experimental technique new zealand rabbit, body weight 2.0-2.4kg is divided into 4 groups at random, 8 every group, that is: and model group, Radix Pseudostellariae extract three dosage group 1.0g/kg, 2g/kg, 4g/kg.Animal auricular vein injecting heparin shoots execution dead behind the 15min, open breast, heart is taken out rapidly, move in the culture dish of the cold Locke perfusate of contain, wash out heart blood as far as possible, after the pruning, under liquid level, rapidly aorta is connected on the sleeve pipe of perfusion, and pricks fixing with toe-in.Carry out perfusion to feed 95% O2 and the Locke liquid of 5%CO2 immediately.Perfusion bottle rise heart 50～60cm, the perfusate temperature keeps 38 ± 0.5 ℃.Three electrodes insert in left and right sides auricle and apex respectively, are connected in two road physiology monitors, continue cardioelectric monitor.Recovering beat of heart and ECG stablize 15min after showing normally, experimentize again, arrhythmia person occurs during this period and discard.
Modeling is adopted and is stopped irritating the multiple again filling damage of ischemia: close the aorta sleeve pipe, make heart stop irritating and causing myocardial ischemia, unclamp cartridge clip behind the 45min, recover the nature perfusion.Medicine joins in the perfusate, has irritated in the multiple beginning 5min that irritates.
2.2 being determined under the heart of coronary perfusion amount collected effluent with funnel and graduated cylinder, the recorded stream liquid outlet quantity is coronary flow.Before stopping irritating, the continuous discharge of measuring the per minute perfusates for 3 times is with the normal groundwater increment of its meansigma methods before as administration.After multiple the filling, the record coronary perfusion amount in 5,10,15,30,45, during 60min.
Measure the vigor of irritating lactic acid dehydrogenase (LDH), creatine kinase (CK) in the fluid 2.3 irritate fluid biochemical measurement biochemical instruments.
2.4 after the tectology checking experiment finished, it was dirty to core, fixing in 10% formalin solution, routine is drawn materials, dyes, is cut into slices, and does the pathology histological examination under optical microscope.
3. experimental result
3.1 Radix Pseudostellariae extract is to the influence of extrasomatic rabbit heart ischemia-reperfusion coronary perfusion amount
The coronary perfusion amount significantly descends when 15min behind the ischemia-reperfusion, after giving Radix Pseudostellariae extract, the Radix Pseudostellariae extract low dose group at 45min and middle and high dosage group the coronary perfusion amount behind 15min apparently higher than model group (P＜0.05), its rate of change and model group be significant difference (P＜0.05 relatively, P＜0.01), show that Radix Pseudostellariae extract can significantly resist the decline of the caused coronary perfusion amount of ischemic injuries, and, be dose-effect relationship preferably along with the effect of increasing of dosage strengthens.The results are shown in Table 3.
Influence (X ± S) (n=6) of table 3 pair extrasomatic rabbit heart ischemia-reperfusion coronary flow
| Group | Dosage (g/k g) | Coronary flow (ml/min) | ||||||
| Before stopping irritating | After multiple the filling | |||||||
| 5min | 10min | 15min | 30min | 45min | 60min | |||
| The model maggot is thousand conopsea extractions too | -- 4 2 1 | 7.0 ±0.6 7.3 ±0.7 7.0 ±0.3 7.0 ±0.2 | 5.0 ±1.8 6.6 ±1.7 6.5 ±1.1 5.2 ±1.1 | 4.5 ±1.6 6.1 ±0.8 6.2 ±0.8 4.7 ±1.3 | 4.2 ±1.1 6.0 ±0.6 * 5.9 ±0.6 * 5.2 ±1.0 | 4.1 ±0.8 5.5 ±0.9 * 5.2 ±0.7 * 5.0 ±1.3 | 3.6 ±0.7 4.7 ±0.7 ** 4.6 ±0.8 * 4.9 ±1.2 * | 3.5 ±0.8 4.7 ±0.89 * 4.8 ±0.7 * 4.6 ±1.2 |
Compare with the model group group
*P＜0.05,
*P＜0.01
3.2 Radix Pseudostellariae extract is to the influence of LDH in the extrasomatic rabbit heart ischemia-reperfusion myocardial perfusion liquid
Give low behind the Radix Pseudostellariae extract, high dose group and irritate behind 45min in 30min, middle dosage group that the LDH vigor significantly is lower than model group (P＜0.05 in the fluid; The rate of change of irritating LDH vigor in the fluid P＜0.01),, behind the small dose group 45min, behind the high dose group 30min all is significantly less than model group (P＜0.05; P＜0.01), shows that Radix Pseudostellariae extract can resist leaking outside of LDH in the ischemia-reperfusion cardiac muscle.The results are shown in Table 4.
Table 4 pair extrasomatic rabbit heart ischemia-reperfusion cardiac muscle is irritated influence (X ± S) (n=6) of LDH in the fluid
| Group | Dosage (g/kg) | LDH(u/L) | ||||||
| Before stopping irritating | After multiple the filling | |||||||
| 5min | 10min | 15min | 30min | 45min | 60min | |||
| The model group Radix Pseudostellariae extract | -- 4 2 1 | 28.5 ±15.8 26.8 ±15.5 24.2 ±10.5 32.0 ±12.5 | 37.0 ±28.9 26.5 ±8.9 37.8 ±7.3 28.8 ±14.5 | 24.6 ±15.0 26.8 ±14.7 33.3 ±10.6 35.6 ±10.3 | 88.0 ±128.6 24.8 ±17.0 29.3 ±18.6 58.7 ±96.7 | 142.6 ±112.9 25.5 ±15.2 * 39.3 ±24.0 * 67.8 ±36.9 | 195.3 ±67.2 32.7 ±21.4 ** 41.2 ±19.6 ** 77.8 ±62.5 * | 216.8 ±89.2 40.7 ±24.3 ** 38.8 ±22.9 ** 103.2 ±80.2 * |
Compare with the model group group
*P＜0.05,
*P＜0.01
3.3 Radix Pseudostellariae extract is irritated the influence of CK in the fluid to extrasomatic rabbit heart ischemia-reperfusion cardiac muscle
After giving Radix Pseudostellariae extract, in, the low dose group heart irritate in the fluid CK vigor after pouring into 45min again, the high dose group heart irritates that the CK vigor all significantly is lower than model group (P＜0.05) in the fluid after pouring into 30min again, and the rate of change of CK (P＜0.05 of comparing with model group that there were significant differences behind high, the middle dosage group 15min; P＜0.01), shows that Radix Pseudostellariae extract leaks outside to the caused myocardium CK of ischemia-reperfusion and has antagonism, and, be certain dose-effect relationship along with the effect of increasing of dosage strengthens.The results are shown in Table 5.
Table 5 Radix Pseudostellariae extract is irritated influence (X ± S) (n=8) of cK in the fluid to extrasomatic rabbit heart ischemia-reperfusion cardiac muscle
| Group | Dosage (g/kg) | CK(u/L) | ||||||
| Before stopping irritating | After multiple the filling | |||||||
| 5min | 10min | 15min | 30min | 45min | 60min | |||
| The model group Radix Pseudostellariae extract | -- 4 2 1 | 117.5 ±67.1 105.2 ±65.6 85.5 ±60.6 110.7 ±51.2 | 69.7 ±83.8 50.8 ±42.7 56.4 ±11.8 40.5 ±7.2 | 78.0 ±65.9 40.2 ±55.6 32.8 ±23.9 50.5 ±26.5 | 266.2 ±273.6 69.8 ±61.4 182.8 ±283.6 67.2 ±28.1 | 550.3 ±507.3 129.5 ±110.2 91.7 ±75.8 * 78.7 ±53.1 * | 829.7 ±638.3 158.0 ±70.6 * 98.7 ±88.0 * 188.7 ±198.4 * | 967.5 ±747.5 258.5 ±161.4 * 241 ±243.6 * 168.0 ±187.4 * |
Compare with the model group group
*P＜0.05,
*P＜0.01
3.4 Radix Pseudostellariae extract is tested the influence of myocardium pathological change to the extrasomatic rabbit heart ischemia-reperfusion
The experimental result prompting, myocardial cell injury is obvious behind the ischemia-reperfusion, with normal group comparing difference remarkable ((P＜0.01), give that cardiomorphology and model group relatively have clear improvement behind the Radix Pseudostellariae extract, necrocytosis, interstitial edema and cell infiltration degree all significantly alleviate (P＜0.05, P＜0.01).Radix Pseudostellariae extract middle and high dosage group cardiomorphology and model group relatively have clear improvement (P＜0.05, P＜0.01).The results are shown in Table 6.
Table 6 pair extrasomatic rabbit heart ischemia-reperfusion is tested influence (X ± S) (n=6) of myocardium pathological change
| Group | The myocardial cell degeneration necrosis | P | Interstitial edema | P | Cell infiltration | P | ||||||||||
| - | + | ++ | +++ | - | + | ++ | +++ | - | + | ++ | +++ | |||||
| The model group Radix Pseudostellariae extract | 1 2 4 | 1 2 5 | 1 2 2 1 | 1 2 | 5 2 | * ** | 1 2 5 | 1 1 1 | 1 1 1 | 5 3 2 | ## ** | 1 3 5 6 | 4 2 1 | 1 1 | * * | |
Compare * P＜0.05, * * P＜0.01 with the model group group.
(-, represents negative, and+expression the positive is kept the score ,+many promptings pathological changes is serious more more)
Experimental example 3: Radix Pseudostellariae is induced the influence of experimental myocardial hypertrophy to the rat coronary ligation
1 experiment material
1.1. experimental apparatus and reagent
Radix Pseudostellariae extract, this research department's independent development is chocolate brown powder, people's consumption is 1～12g (extract)/10～120g crude drug/60kg.
Animal respirator (HX-300 Chengdu Tai Ming Science and Technology Ltd.)
Electrocardiograph (medical electronic apparatus factory of ECG-11D Ke Meisi group)
High speed electric centrifuge (the sub-company limited of Jintan, YXJ-2A Jiangsu hundred million energisings)
TDL-4ZB table-type low-speed autobalance centrifuge (Hunan Xingke Scientific Instrument Co., Ltd.)
Portable High pressure steam sterilizer (the bright forever Medical Instruments in model GMSX-280 Beijing factory)
Electronic balance (numbering: 04005955 model: Tianjin, island IBLZ20H)
Malonaldehyde (MDA) test kit (bio-engineering research institute is built up in Nanjing)
Superoxide dismutase (SOD) test kit (bio-engineering research institute is built up in Nanjing)
Total antioxidant capacity (T-AOC) test kit (bio-engineering research institute is built up in Nanjing)
1.2 laboratory animal
The healthy SD rat, male and female half and half, body weight 200～250g; Guiyang Medical College experimental animal center, quality certification SCXK (Guizhou Province) 2002-2001.Animal is raised with test full-valence pellet feed (Guiyang Medical College experimental animal center provides), freely drinks water 22 ± 2 ℃ of room temperatures.
2. myocardial hypertrophy model copy and animal grouping
Rat is divided into 5 groups at random by body weight, that is: normal group, model group, Radix Pseudostellariae three dosage group: 2g/kg, 4g/kg, 8g/kg.10% chloral hydrate lumbar injection fiber crops shallow (0.3ml/100g), back of the body position is fixed on the operating-table, and it is subcutaneous to insert extremity with needle electrode, observed and recorded standard limbs II lead electrocardiogram.Be connected to artificial respirator with more than half rubber bulb (size is just in time entangled the rat incidence), practice artificial respiration.Respiratory frequency 120 times/minute, tidal volume 18～25ml/kg breathes time-histories than 2: 1.The chest unhairing, sterilization is vertically cut the about 2cm of skin along left mid-clavicular line, successively separates subcutaneous tissue in the 4th or the 5th intercostal passivity, and muscle is made purse string suture, and is standby.Oxygen supply is separated the 3rd intercostal space, opens the thoracic cavity, cuts off pericardium, gently presses the right side thorax, extrudes heart.With suture ligation ramus descendens anterior arteriae coronariae sinistrae in Coronary vein place between arterial cone and left auricle, heart is put back to the thoracic cavity rapidly.Drain gas, seam closes the thoracic cavity, recovers the rat autonomous respiration, stops the artificial respiration.Obviously raise more than lasting half an hour with electrocardiogram limbs (II leads) the ST section of leading.More than operate under the aseptic condition and carry out, postoperative drips two anti-(penicillin adds streptomycin) 4～5, two anti-0.1ml/ days of intramuscular injection afterwards, infection for three days on end in incision.The sham operated rats rat is only at the arteria coronaria underpass, not ligation arteria coronaria, and all the other steps are the same.Each is organized the continuous irrigation stomach and is administered into the scheduled time, after rat is weighed, and common carotid artery sacrificed by exsanguination rat.The centrifugal 10min of 2000r/min gets serum and preserves in-20 ℃ of cryogenic refrigerators, presses the test kit description and measures blood mesophytization indexs such as SOD, MDA, T-AOD.Take out heart, clean, separate left ventricle, right ventricle, calculate cardiac index, ventricle index.
3. result
The prompting of this experimental studies results, in 2 weeks of coronary ligation, model group rat cardiac index and left room index and sham operated rats relatively show no obvious abnormalities; Coronary ligation is after 4 weeks, and model group rat cardiac index, left ventricle index are all apparently higher than sham operated rats (P＜0.05); 4 weeks formed the myocardial hypertrophy model behind the prompting rat coronary ligation.The Radix Pseudostellariae extract successive administration is after 4 weeks, and two dosage groups can obviously reduce rat cardiac index and left room index.This result of study prompting Radix Pseudostellariae extract has the certain protection effect to the rat chronic myocardial infarction model.The testing result prompting of serum T-AOC and SOD, in 4 weeks of continuous use, heavy dose of group obviously improves level and the SOD vigor of T-AOC in the serum.The results are shown in Table 7 with table 8.
Table 7 Radix Pseudostellariae extract is to the influence of chronic myocardial infarction model cardiac index
| Group | Dosage (mg/mg) | 2 weeks | 4 weeks | ||||
| Cardiac index | Left side room index | Right ventricle's index | Cardiac index | Left side room index | Right ventricle's index | ||
| Sham operated rats | -- | 0.403 ±0.053 | 0.242 ±0.026 | 0.090 ±0.020 | 0.372 ±0.060 | 0.234 ±0.030 | 0.076 ±0.027 |
| Model group | -- | 0.390 ±0.026 | 0.247 ±0.013 | 0.070 ±0.017 | 0.468 ±0.074 # | 0.296 ±0.041 # | 0.088 ±0.020 |
| Radix Pseudostellariae extract | 8 | 0.373 ±0.036 | 0.241 ±0.018 | 0.078 ±0.022 | 0.343 ±0.022 ** | 0.217 ±0.013 * | 0.066 ±0.009 * |
| 4 | 0.394 ±0.322 | 0.255 ±0.028 | 0.079 ±0.012 | 0.368 ±0.042 * | 0.239 ±0.031 * | 0.069 ±0.017 | |
| 2 | 0.432 ±0.087 | 0.266 ±0.046 | 0.078 ±0.031 | 0.355 ±0.037 ** | 0.240 ±0.021 * | 0.056 ±0.010 ** | |
Compare with model group
#P＜0.05,
##P＜0.01; Compare * P＜0.05, * * P＜0.01 with the normal control group.
Table 8 Radix Pseudostellariae extract is to the influence of chronic myocardial infarction model blood T-AOC and SOD
| Group | Dosage (mg/mg) | 2 weeks | 4 weeks | ||
| T-AOC | SOD | T-AOC | SOD | ||
| Sham operated rats | -- | 13.916±3.645 | 240.23±9.65 | 13.299±1.530 | 230.86±11.04 |
| Model group | -- | 18.685±6.001 | 216.48±17.96 # | 10.483±2.623 # | 206.44±7.14 # |
| Radix Pseudostellariae extract | 8 | 14.615±2.986 | 253.03±5.52 ** | 14.533±1.822 * | 243.50±9.32 ** |
| 4 | 13.526±4.130 | 229.28±8.51 | 8.531±5.250 | 216.06±17.45 | |
| 2 | 15.326±6.253 | 222.50±9.86 | 11.059±1.886 | 212.63±14.02 | |
Compare with model group
#P＜0.05; Compare with sham operated rats
*P＜0.05,
*P＜0.01.
The specific embodiment
Embodiment 1: capsule
Get Radix Pseudostellariae 1200g, preparation technology makes fine powder or granule routinely, mixing, 1000 of No. 0 capsules of packing into, each 1-4 grain, 1-4 time on the one.
Embodiment 2: injection
Get Radix Pseudostellariae 800g, preparation technology makes 1000 of injections routinely, and each one, 1-4 time on the one.
Embodiment 3: oral cavity disintegration tablet
Get Radix Pseudostellariae 1200g, preparation technology makes 1000 of oral cavity disintegration tablets routinely, each 1-4 sheet, 1-4 time on the one.
Embodiment 4: the compound granular agent
Get Radix Pseudostellariae 1500g, Ramulus Cinnamomi 320g, preparation technology makes 200 bags routinely, every bag 5 gram, each 0.5-3 bag, 1-4 time on the one.
Embodiment 5: compound tablet
Get Radix Pseudostellariae 1300g, Herba Menthae 150g, preparation technology makes 1000 in tablet routinely.Each 1-4 sheet, 1-4 time on the one.
Embodiment 6: compound dripping pill
Get Radix Pseudostellariae 400g, Borneolum Syntheticum 20g, preparation technology makes 1000 of drop pill routinely.Each 1-8 grain, 1-4 time on the one.
Embodiment 7: compound capsules
Get Radix Pseudostellariae 1500g, Radix Astragali 800g, preparation technology makes 1000 of soft capsules routinely.Each 1-6 grain, 1-4 time on the one.
Embodiment 8: the compound recipe oral cavity disintegration tablet
Get Radix Pseudostellariae 1500g, Radix Salviae Miltiorrhizae 600g, Rhizoma Corydalis 600g, preparation technology makes 1000 of oral cavity disintegration tablets routinely.Each 1-6 sheet, 1-4 time on the one.
Claims (4)
1. the application of Radix Pseudostellariae in preparation treatment cardiovascular disease medicine.
2. the application of Radix Pseudostellariae according to claim 1 in preparation treatment cardiovascular disease medicine, it is characterized in that: Radix Pseudostellariae is applied to prepare the medicine for the treatment of cardiovascular disease, be to be raw material with the Radix Pseudostellariae, technology obtains the Radix Pseudostellariae total extract through extraction routinely, and then add corresponding adjuvant, be prepared into any oral or non-peroral dosage form that is suitable for using clinically, as powder, tablet, granule, capsule, microcapsule, pill, drop pill, soft capsule, oral liquid, syrup, dispersible tablet, spray or aerosol, suppository, intravenous injection, intramuscular dose, ointment, inhalant, gel; Used adjuvant comprises conventional solvent, disintegrating agent, correctives, antiseptic, coloring agent, binding agent, lubricant, wetting agent, thickening agent, solubilizing agent, substrate.
3. the application of Radix Pseudostellariae according to claim 1 and 2 in preparation treatment cardiovascular disease medicine, it is characterized in that: Radix Pseudostellariae is used to prepare the medicine for the treatment of cardiovascular disease, can also forms compound medicine with one or more the medicine in wind-cold-dispersing medicinal, wind-heat-dispersing medicinal, drugs for dispelling internal cold, QI regulating medicine, stasis-resolving hemostatic, drug for invigorating blood circulation and eliminating stasis, awaking drug, the qi-restoratives medicine.
4. the application of Radix Pseudostellariae according to claim 3 in preparation treatment cardiovascular disease medicine, it is characterized in that: described wind-cold-dispersing medicinal is Ramulus Cinnamomi, Herba Asari or Rhizoma Zingiberis Recens; Described wind-heat-dispersing medicinal is Herba Menthae, Radix Puerariae or Rhizoma Cimicifugae; Described drugs for dispelling internal cold is Radix Aconiti Lateralis Preparata, Rhizoma Zingiberis, Cortex Cinnamomi, Fructus Evodiae, Pericarpium Zanthoxyli or Flos Caryophylli; Described QI regulating medicine is Pericarpium Citri Reticulatae, Pericarpium Citri Reticulatae Viride, the Radix Aucklandiae, Rhizoma Cyperi, Fructus Toosendan or the Radix Linderae; Described stasis-resolving hemostatic is Radix Notoginseng, Pollen Typhae or Radix Rubiae; Described drug for invigorating blood circulation and eliminating stasis is Rhizoma Chuanxiong, Rhizoma Corydalis, Radix Curcumae, Rhizoma Curcumae Longae, Olibanum, Myrrha, Radix Salviae Miltiorrhizae, Flos Carthami, Semen Persicae, Herba Leonuri, cattle, Radix Ilicis Pubescentis, Rhizoma Curcumae, Hirudo, Sanguis Draxonis or Semen Strychni; Described awaking drug is Moschus, Borneolum Syntheticum or Styrax; Described qi-restoratives medicine is Radix Ginseng, Radix Panacis Quinquefolii, Radix Codonopsis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Rhizoma Dioscoreae, Radix Glycyrrhizae or Radix Et Caulis Acanthopanacis Senticosi.
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| CN 200610201099 CN1957967A (en) | 2006-11-16 | 2006-11-16 | Application of pseudostellaria root for preparing medication of treating cardiovascular disease |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102342505A (en) * | 2011-08-31 | 2012-02-08 | 杨素华 | Production method of heterophylly falsestarwort root full value nutrient powder |
| CN102940772A (en) * | 2012-11-22 | 2013-02-27 | 林久全 | Drug for treating angina pectoris and preparation method of drug |
| CN103989810A (en) * | 2010-05-27 | 2014-08-20 | 王伟 | A composite traditional Chinese medicine used for treating coronary heart disease cardiac insufficiency and a preparing method thereof |
-
2006
- 2006-11-16 CN CN 200610201099 patent/CN1957967A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103989810A (en) * | 2010-05-27 | 2014-08-20 | 王伟 | A composite traditional Chinese medicine used for treating coronary heart disease cardiac insufficiency and a preparing method thereof |
| CN103989810B (en) * | 2010-05-27 | 2016-05-11 | 王伟 | A kind of tcm components that heart function of coronary heart disease is incomplete and preparation method thereof that is used for the treatment of |
| CN102342505A (en) * | 2011-08-31 | 2012-02-08 | 杨素华 | Production method of heterophylly falsestarwort root full value nutrient powder |
| CN102940772A (en) * | 2012-11-22 | 2013-02-27 | 林久全 | Drug for treating angina pectoris and preparation method of drug |
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Open date: 20070509 |