CN100334208C - A method for extracting superoxide dismutase - Google Patents
A method for extracting superoxide dismutase Download PDFInfo
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- CN100334208C CN100334208C CNB2004100313619A CN200410031361A CN100334208C CN 100334208 C CN100334208 C CN 100334208C CN B2004100313619 A CNB2004100313619 A CN B2004100313619A CN 200410031361 A CN200410031361 A CN 200410031361A CN 100334208 C CN100334208 C CN 100334208C
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- sod
- leaf
- stem
- false lychee
- alkaline
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- 102000019197 Superoxide Dismutase Human genes 0.000 title claims abstract description 61
- 108010012715 Superoxide dismutase Proteins 0.000 title claims abstract description 61
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000000047 product Substances 0.000 claims abstract description 23
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 22
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 22
- 239000006228 supernatant Substances 0.000 claims abstract description 11
- 229940032362 superoxide dismutase Drugs 0.000 claims description 55
- 235000015742 Nephelium litchi Nutrition 0.000 claims description 40
- 239000000287 crude extract Substances 0.000 claims description 13
- 150000003839 salts Chemical class 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000001556 precipitation Methods 0.000 claims description 7
- 239000000706 filtrate Substances 0.000 claims description 6
- 244000108452 Litchi chinensis Species 0.000 claims 5
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-M dihydrogenphosphate Chemical compound OP(O)([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-M 0.000 claims 1
- 150000003016 phosphoric acids Chemical class 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 239000003513 alkali Substances 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 229910017053 inorganic salt Inorganic materials 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 244000183278 Nephelium litchi Species 0.000 description 35
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 12
- 239000000284 extract Substances 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 238000006701 autoxidation reaction Methods 0.000 description 6
- 229940079877 pyrogallol Drugs 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 238000013467 fragmentation Methods 0.000 description 4
- 238000006062 fragmentation reaction Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 3
- 238000012870 ammonium sulfate precipitation Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000008057 potassium phosphate buffer Substances 0.000 description 2
- 238000005185 salting out Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 101800000263 Acidic protein Proteins 0.000 description 1
- 235000009899 Agrostemma githago Nutrition 0.000 description 1
- 101000836247 Aquifex pyrophilus Superoxide dismutase [Fe] Proteins 0.000 description 1
- 244000189799 Asimina triloba Species 0.000 description 1
- 235000006264 Asimina triloba Nutrition 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 241001507936 Chaenomeles Species 0.000 description 1
- 240000000425 Chaenomeles speciosa Species 0.000 description 1
- 101001091385 Homo sapiens Kallikrein-6 Proteins 0.000 description 1
- 102100034866 Kallikrein-6 Human genes 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- -1 Oxygen free radical Chemical class 0.000 description 1
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 244000178320 Vaccaria pyramidata Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000003811 acetone extraction Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003471 anti-radiation Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 238000007323 disproportionation reaction Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- Enzymes And Modification Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a method for extracting a crude superoxide dismutase (SOD) product from fruits of stauntonvine, which comprises: crushing the stauntonvine, adding alkali inorganic salt at the same time, and adjusting ph to subacidity; filtering and centrifuging; adding acetone to the supernatant, and centrifuging to obtain precipitate; airing. The present invention is suitable for effectively extracting the crude SOD product from the fruits of the stauntonvine to increase the yield of the crude SOD product. The stauntonvine fruit SOD extracted by using the method has good application prospect in medicine and food.
Description
Technical field
The present invention relates to a kind of method of extracting superoxide-dismutase, relate in particular to a kind of method of from the Stem and leaf of False Lychee fruit, extracting superoxide-dismutase.
Background technology
Superoxide-dismutase (SOD) extensively is present in the organism, and the disproportionation reaction of Catalytic Oxygen free radical has strong toxic superoxide radical thereby remove to organism, and organism is shielded.Therefore SOD has anti-inflammatory, anti-ageing, Antiradiation injury and antitumor action, and has been applied to fields such as medicine, makeup and food.
Commodity SOD mainly comes from animal blood at present, also has research that many plants (as vegetables, herbal medicine etc.) have been carried out the experiment that SOD extracts.Chinese patent application 93103820.0 is the extracting method about tobacco superoxide-dismutase (SOD): utilize potassium phosphate buffer to make extract, regulate pH to 4.0 with acetic acid and remove basic protein, regulate pH to 7.5 with NaOH and remove acidic protein, utilize ammonium sulfate precipitation to extract SOD, obtain crude zyme preparation.Up to the present, the main technique of extracting SOD comprise saltout, organic solvent extraction, chromatography, ultrafiltration etc.
Because the pH value of Stem and leaf of False Lychee fruit is difficult to regulate pH with damping fluid below 3, use existing technology and processing condition, be difficult to from the Stem and leaf of False Lychee fruit, extract SOD.Do not see the report that from the Stem and leaf of False Lychee fruit, extracts SOD up to now as yet.
Summary of the invention
The purpose of this invention is to provide a kind of method of from the Stem and leaf of False Lychee fruit, extracting superoxide-dismutase (SOD), use this method, can from the Stem and leaf of False Lychee fruit, extract the SOD raw product effectively, improve the yield of SOD raw product.This is that other SOD extracting method institute is inaccessiable.
The method of extracting superoxide-dismutase (SOD) raw product from the Stem and leaf of False Lychee fruit of the present invention realizes as follows:
A. the water that adds 1～3 times of Stem and leaf of False Lychee weight in the Stem and leaf of False Lychee fruit, and alkaline, inorganic salts, the add-on of alkaline, inorganic salts is 3～9% of a Stem and leaf of False Lychee weight, broken Stem and leaf of False Lychee fruit, crude extract pH are 3～6;
B. above-mentioned crude extract is filtered, filtrate is centrifugal;
C. get supernatant liquor, and add the acetone soln of 1～4 times of supernatant liquor volume;
D. centrifugal, get precipitation, drying.
Among the step a of the present invention, alkaline, inorganic salts is preferably PO
4 3-Salt, HPO
4 2-Salt, H
2PO
4 -Salt, CO
3 2-Salt or HCO
3 -Salt, for example Na
3PO
4, Na
2HPO
4, NaH
2PO
4, Na
2CO
3, NaHCO
3Deng.Crude extract pH is preferably 3～5.
The plant material that the present invention relates to is the Stem and leaf of False Lychee fruit, is rosaceous plant chaenomeles lagenaria (cockle pawpaw, Fructus Chaenomelis)
Chaenomeles LagenariaMature fruit, fruit is very sour, pH is about 2.7.
According to the method for from the Stem and leaf of False Lychee fruit, extracting superoxide-dismutase (SOD) raw product of the present invention, in broken Stem and leaf of False Lychee, add alkaline, inorganic salts, regulate pH to slightly acidic; The add-on of alkaline, inorganic salts is 3～9% of a Stem and leaf of False Lychee weight, regulates crude extract pH to 3～6; Stem and leaf of False Lychee is very sour, and the pH of Stem and leaf of False Lychee fresh fruit is about 2.7, and broken Stem and leaf of False Lychee adds the water of 1～3 times of Stem and leaf of False Lychee weight, and the pH of its crude extract is about 3.0, and in pH was about 3.0 SOD crude extract, the activity of SOD was very low; According to ordinary method, slightly carry SOD with buffer systems such as phosphoric acid buffers, buffer system can be destroyed by the acidic substance in the Stem and leaf of False Lychee, and the pH that causes extracting solution does not reach buffering effect near 3.0; Have only to add a large amount of alkaline, inorganic salts, the pH that could guarantee extracting solution is near slightly acidic, thereby guarantees can detect in extracting solution higher SOD activity.
According to the method for extracting SOD from the Stem and leaf of False Lychee fruit of the present invention, the volume that adds acetone is 1～4 times of supernatant liquor volume; In animal blood, extract SOD, generally can use acetone and other organic solvent, be used to remove lipid-soluble substance; In plant SOD extracts, use ammonium sulfate salting-out process, still do not use acetone method; In Stem and leaf of False Lychee SOD extracted, ammonium sulfate salting-out process was inoperative, is difficult to detect the SOD enzymic activity in the ammonium sulfate precipitation extracting solution, but used the acetone extraction of 1～4 times of volume of supernatant liquor, can detect highly active SOD in extracting solution.
The present invention has following advantage:
1. prior art can't be extracted SOD from the Stem and leaf of False Lychee fruit, and can extract SOD in the Stem and leaf of False Lychee effectively with technology of the present invention.
2. technology of the present invention is simple, easy to operate, and cost is low, suitable for mass production.
3. the SOD raw product that makes of the present invention has very high yield, the general equal bright tissue of 14U/g that reaches.
Embodiment
Can further know understanding the present invention by embodiment given below, but they are not to be limitation of the invention.
Embodiment 1: the extraction of Stem and leaf of False Lychee SOD raw product
Stem and leaf of False Lychee 100kg, preliminary broken, add 150kg distilled water, add 6kg Na
2HPO
4, fragmentation, crude extract pH is 4.8; 120 order gauzes filter, and 4 ℃ of following centrifugal 15min of filtrate 7000rpm get supernatant liquor 220L; Add acetone 440L, mixing; 4 ℃ of following centrifugal 15min of 7000rpm get precipitation, dry, and are the SOD raw product.Utilize pyrogallol autoxidation method to measure SOD activity in the raw product, yield is the bright tissue of 15.3U/g.This extracting solution has absorption peak at the 280nm place; At 320nm to 540nm place absorption peak is arranged also, whole absorption peak is milder, and maximum absorption band is at the 340nm place.The spectrometry result shows that the enzyme that is extracted is the Fe-SOD enzyme.
Utilize pyrogallol autoxidation method to measure SOD activity in the raw product, yield is the bright tissue of 15.3U/g.
Embodiment 2: the extraction of Stem and leaf of False Lychee SOD raw product
Stem and leaf of False Lychee 100kg, preliminary broken, add 100kg distilled water, add 3kg NaHCO
3, fragmentation, crude extract pH is 3.4; 120 order gauzes filter, and 4 ℃ of following centrifugal 15min of filtrate 7000rpm get supernatant liquor 170 L; Add acetone 170L, mixing; 4 ℃ of following centrifugal 15min of 7000rpm get precipitation, dry, and are the SOD raw product.Utilize pyrogallol autoxidation method to measure SOD activity in the raw product, yield is the bright tissue of 13.7U/g.
Embodiment 3: the extraction of Stem and leaf of False Lychee SOD raw product
Stem and leaf of False Lychee 100kg, preliminary broken, add 150kg distilled water, add 9kg Na
2HPO
4, fragmentation, crude extract pH is 5.8; 120 order gauzes filter, and 4 ℃ of following centrifugal 15min of filtrate 7000rpm get supernatant liquor 220L; Add acetone 880L, mixing; 4 ℃ of following centrifugal 15min of 7000rpm get precipitation, dry, and are the SOD raw product.Utilize pyrogallol autoxidation method to measure SOD activity in the raw product, yield is the bright tissue of 15.1U/g.
Embodiment 4: the extraction of Stem and leaf of False Lychee SOD raw product
Stem and leaf of False Lychee 100kg, preliminary broken, add 300kg distilled water, add 9kg K
2HPO
4, fragmentation, crude extract pH is 6.0; 120 order gauzes filter, and 4 ℃ of following centrifugal 15min of filtrate 7000rpm get supernatant liquor 370L; Add acetone 800L, mixing; 4 ℃ of following centrifugal 15min of 7000rpm get precipitation, dry, and are the SOD raw product.Utilize pyrogallol autoxidation method to measure SOD activity in the raw product, yield is the bright tissue of 14.2U/g.
Utilize pyrogallol autoxidation method to measure the SOD activity that prior art (is that 93103820.0 technology is an example with application number) and the art of this patent are extracted in each step, the result shows with prior art can't extract SOD at last from the Stem and leaf of False Lychee fruit, and can extract Stem and leaf of False Lychee SOD effectively with the art of this patent.
The Stem and leaf of False Lychee SOD yield that table 1. prior art and the art of this patent are extracted (the bright tissue of U/g)
| Step | Prior art and yield | The art of this patent and yield | ||
| The first step | Potassium phosphate buffer is slightly carried | 13.5 | Distilled water+alkaline, inorganic salts is slightly carried | 310.7 |
| Second step | Ammonium sulfate precipitation | 0 | Acetone precipitation | 14.2 |
| Last yield | 0 | Last yield | 14.2 | |
Claims (4)
1. method of from the Stem and leaf of False Lychee fruit, extracting the superoxide-dismutase raw product, this method realizes as follows:
A. the water that adds 1～3 times of Stem and leaf of False Lychee weight in the Stem and leaf of False Lychee fruit, and alkaline, inorganic salts, this alkaline, inorganic salts is selected from phosphoric acid salt, hydrophosphate, dihydrogen phosphate, carbonate or supercarbonate, and the add-on of this alkaline, inorganic salts is 3～9% of a Stem and leaf of False Lychee weight, broken Stem and leaf of False Lychee fruit, crude extract pH is 3～6;
B. above-mentioned crude extract is filtered, filtrate is centrifugal;
C. get the supernatant liquor after centrifugal among the b, and add the acetone of 1～4 times of supernatant liquor volume;
D. the solution centrifugal that step c is obtained is got precipitation, and dry back obtains the superoxide-dismutase raw product.
2. method according to claim 1 is characterized in that the alkaline, inorganic salts that adds is K
2HPO
4
3. method according to claim 1 is characterized in that the alkaline, inorganic salts that adds is selected from Na
3PO
4, Na
2HPO
4, NaH
2PO
4, Na
2CO
3Or NaHCO
3
4. according to the arbitrary described method of claim 1-3, it is characterized in that adjusting pH to 3～5 of crude extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100313619A CN100334208C (en) | 2004-03-25 | 2004-03-25 | A method for extracting superoxide dismutase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100313619A CN100334208C (en) | 2004-03-25 | 2004-03-25 | A method for extracting superoxide dismutase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1673364A CN1673364A (en) | 2005-09-28 |
| CN100334208C true CN100334208C (en) | 2007-08-29 |
Family
ID=35046139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100313619A Expired - Fee Related CN100334208C (en) | 2004-03-25 | 2004-03-25 | A method for extracting superoxide dismutase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100334208C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101962634A (en) * | 2010-09-15 | 2011-02-02 | 安徽农业大学 | Methods for extracting papain and superoxide dismutase (SOD) crude enzyme from comman floweringquince fruit |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102925416A (en) * | 2012-11-19 | 2013-02-13 | 景志刚 | Method for extracting superoxide dismutase from blood of mammal |
| CN108004217A (en) * | 2017-11-28 | 2018-05-08 | 韦俊超 | The extraction of pawpaw SOD a kind of and purification process |
-
2004
- 2004-03-25 CN CNB2004100313619A patent/CN100334208C/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| 沙棘超氧化物歧化酶（SOD）的提取与化学修饰 李扬等，内蒙古农业大学学报，第23卷第3期 2002 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101962634A (en) * | 2010-09-15 | 2011-02-02 | 安徽农业大学 | Methods for extracting papain and superoxide dismutase (SOD) crude enzyme from comman floweringquince fruit |
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| Publication number | Publication date |
|---|---|
| CN1673364A (en) | 2005-09-28 |
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