CL2016003010A1 - Sistema de expresión optogenético en levadura - Google Patents
Sistema de expresión optogenético en levaduraInfo
- Publication number
- CL2016003010A1 CL2016003010A1 CL2016003010A CL2016003010A CL2016003010A1 CL 2016003010 A1 CL2016003010 A1 CL 2016003010A1 CL 2016003010 A CL2016003010 A CL 2016003010A CL 2016003010 A CL2016003010 A CL 2016003010A CL 2016003010 A1 CL2016003010 A1 CL 2016003010A1
- Authority
- CL
- Chile
- Prior art keywords
- optogenetic
- expression system
- yeast
- transcription
- gene
- Prior art date
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 102000040945 Transcription factor Human genes 0.000 abstract 2
- 108091023040 Transcription factor Proteins 0.000 abstract 2
- 108091008695 photoreceptors Proteins 0.000 abstract 2
- 238000013518 transcription Methods 0.000 abstract 2
- 230000035897 transcription Effects 0.000 abstract 2
- 238000006471 dimerization reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La invención corresponde a un sistema de expresión optogenético en levadura y un método para regular la expresión de un gen en una célula hospedera, donde el sistema de expresión optogenético comprende al menos dos fotorreceptores, o proteínas sensibles a luz distintas que pueden formar un factor de transcripción quimérico, donde una vez que el sistema se expone a la luz, se genera un cambio conformacional que permite la dimerización entre ambos fotorreceptores distintos, formándose así el factor de transcripción, permitiendo la unión de dicho factor de transcripción al promotor, generando así la transcripción del gen de interés.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CL2016003010A CL2016003010A1 (es) | 2016-11-24 | 2016-11-24 | Sistema de expresión optogenético en levadura |
| PCT/CL2017/050066 WO2018094542A1 (es) | 2016-11-24 | 2017-11-15 | Sistema de expresión optogenético en levadura |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CL2016003010A CL2016003010A1 (es) | 2016-11-24 | 2016-11-24 | Sistema de expresión optogenético en levadura |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CL2016003010A1 true CL2016003010A1 (es) | 2018-11-09 |
Family
ID=62194645
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CL2016003010A CL2016003010A1 (es) | 2016-11-24 | 2016-11-24 | Sistema de expresión optogenético en levadura |
Country Status (2)
| Country | Link |
|---|---|
| CL (1) | CL2016003010A1 (es) |
| WO (1) | WO2018094542A1 (es) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102643852B (zh) * | 2011-02-28 | 2015-04-08 | 华东理工大学 | 光可控的基因表达系统 |
| US20160326219A1 (en) * | 2013-12-13 | 2016-11-10 | Ist Austria (Institute Of Science And Technology Austria) | Optically activated receptors |
-
2016
- 2016-11-24 CL CL2016003010A patent/CL2016003010A1/es unknown
-
2017
- 2017-11-15 WO PCT/CL2017/050066 patent/WO2018094542A1/es active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2018094542A1 (es) | 2018-05-31 |
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