CL2013001596A1 - Proceso de purificacion y estabilizacion de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); y uso de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3). - Google Patents

Proceso de purificacion y estabilizacion de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); y uso de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3).

Info

Publication number
CL2013001596A1
CL2013001596A1 CL2013001596A CL2013001596A CL2013001596A1 CL 2013001596 A1 CL2013001596 A1 CL 2013001596A1 CL 2013001596 A CL2013001596 A CL 2013001596A CL 2013001596 A CL2013001596 A CL 2013001596A CL 2013001596 A1 CL2013001596 A1 CL 2013001596A1
Authority
CL
Chile
Prior art keywords
gadh
enzyme
gluconate dehydrogenase
stabilization
purification
Prior art date
Application number
CL2013001596A
Other languages
English (en)
Inventor
Rodriguez Pablo Alonso
Fernandez Luis Manuel Quiros
De La Torre Sonia Castañon
Susperregui Ainara Crespo
Del Rio Sonia Maza
Original Assignee
Biolan Microbiosensores S L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biolan Microbiosensores S L filed Critical Biolan Microbiosensores S L
Publication of CL2013001596A1 publication Critical patent/CL2013001596A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
    • C12H1/12—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation
    • C12H1/14—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation with non-precipitating compounds, e.g. sulfiting; Sequestration, e.g. with chelate-producing compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/99—Oxidoreductases acting on the CH-OH group of donors (1.1) with other acceptors (1.1.99)
    • C12Y101/99003—Gluconate 2-dehydrogenase (acceptor) (1.1.99.3)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Food Science & Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
CL2013001596A 2010-12-10 2013-06-05 Proceso de purificacion y estabilizacion de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); y uso de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3). CL2013001596A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201031819A ES2387661B1 (es) 2010-12-10 2010-12-10 Proceso de purificación y estabilización de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); y uso de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3).

Publications (1)

Publication Number Publication Date
CL2013001596A1 true CL2013001596A1 (es) 2014-04-11

Family

ID=46206647

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2013001596A CL2013001596A1 (es) 2010-12-10 2013-06-05 Proceso de purificacion y estabilizacion de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); y uso de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3).

Country Status (5)

Country Link
US (1) US20130260407A1 (es)
EP (1) EP2650363A4 (es)
CL (1) CL2013001596A1 (es)
ES (1) ES2387661B1 (es)
WO (1) WO2012076738A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106148257B (zh) * 2015-04-13 2019-08-13 中国科学院上海高等研究院 改造的克雷伯氏肺炎杆菌及其生产葡萄糖酸的应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3928209A1 (de) * 1989-08-25 1991-02-28 Boehringer Mannheim Gmbh D-malat-bestimmung
DE3936757A1 (de) * 1989-11-04 1991-05-08 Forschungszentrum Juelich Gmbh Verfahren zur gewinnung von sorbit und gluconsaeure bzw. gluconat und dafuer geeignete zellmassen
US5861295A (en) * 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
KR100257117B1 (ko) * 1997-09-25 2000-05-15 박호군 세포막 결합 글루코네이트 탈수소 효소 및 그 유전자와 형질전환된 재조합 대장균을 이용한 2-케토-d-글루코네이트로의 생산
AU4312201A (en) * 1999-12-10 2001-06-18 Genespan Corporation Isolation and purification of nucleic acids
WO2001048117A1 (fr) * 1999-12-24 2001-07-05 Otsuka Pharmaceutical Co., Ltd. Compositions seches contenant un acide amine hydrophobe
DK1383903T3 (da) * 2001-04-04 2009-03-23 Genencor Int Fremgangsmåder til fremstilling af askorbinsyre-intermediater i vært-celler
BRPI0513061B8 (pt) * 2004-07-07 2021-05-25 Dsm Ip Assests Bv gtp ciclohidrolase ii modificada selecionada de bacillus, polinucleotídeo, célula hospedeira, processo para a produção de riboflavina, um precursor de riboflavina, fmn, fad ou um derivado destes e uso de uma gtp ciclohidrolase ii modificada ou de um polinucleotídeo
CA2589346A1 (en) * 2004-12-09 2006-06-15 Dow Global Technologies Inc. Enzyme stabilization
US20080003628A1 (en) * 2006-03-31 2008-01-03 Toyo Boseki Kabushiki Kaisha Method for enhancing stability of a composition comprising soluble glucose dehydrogenase (gdh)
CN101589155A (zh) * 2007-01-11 2009-11-25 丹尼斯科美国公司 在含粗甘油的培养基中生产酶
ES2312282B1 (es) 2007-08-02 2010-02-08 Universidad De Cadiz Metodo de atrapamiento e inmovilizacion de enzimas oxidoreductasas y sus usos.
ES2315197B1 (es) 2007-09-14 2010-02-08 Universidad De Cadiz. Nuevo biosensor amperometrico, procedimiento de fabricacion y usos.
GB0812041D0 (en) * 2008-07-02 2008-08-06 Enigma Diagnostics Ltd Compositions
WO2010080407A2 (en) * 2008-12-19 2010-07-15 Novozymes, Inc. Methods for increasing hydrolysis of cellulosic material
AU2009337193A1 (en) * 2008-12-24 2011-07-07 Comvita New Zealand Limited Medical and nutritional formulations

Also Published As

Publication number Publication date
ES2387661A1 (es) 2012-09-27
EP2650363A4 (en) 2014-10-15
US20130260407A1 (en) 2013-10-03
ES2387661B1 (es) 2013-10-31
EP2650363A1 (en) 2013-10-16
WO2012076738A1 (es) 2012-06-14

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