CL2012002634A1 - Microorganismo transgenico que comprende por lo menos un gen que codifica la glucosa-6-fosfato deshidrogenasa, por lo menos un gen que codifica 6-fosfogluconolactonasa y por lo menos un gen heterologo que codifica un producto no nativo de interes; levadura oleaginosa transgenica; y metodo para la produccion de un producto no nativo de interes. - Google Patents

Microorganismo transgenico que comprende por lo menos un gen que codifica la glucosa-6-fosfato deshidrogenasa, por lo menos un gen que codifica 6-fosfogluconolactonasa y por lo menos un gen heterologo que codifica un producto no nativo de interes; levadura oleaginosa transgenica; y metodo para la produccion de un producto no nativo de interes.

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Publication number
CL2012002634A1
CL2012002634A1 CL2012002634A CL2012002634A CL2012002634A1 CL 2012002634 A1 CL2012002634 A1 CL 2012002634A1 CL 2012002634 A CL2012002634 A CL 2012002634A CL 2012002634 A CL2012002634 A CL 2012002634A CL 2012002634 A1 CL2012002634 A1 CL 2012002634A1
Authority
CL
Chile
Prior art keywords
gene encoding
interest
native product
transgenic
production
Prior art date
Application number
CL2012002634A
Other languages
English (en)
Inventor
Seung Pyo Hong
Zhixiong Xue
Quinn Qun Zhu
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of CL2012002634A1 publication Critical patent/CL2012002634A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/66—Preparation of oxygen-containing organic compounds containing the quinoid structure

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Microorganismo transgénico que comprende por lo menos un gen que codifica la glucosa-6-fosfato deshidrogenasa, por lo menos un gen que codifica 6-fosfogluconolactonasa y por lo menos un gen heterólogo que codifica un producto no nativo de interés; levadura oleaginosa transgénica; y método para la producción de un producto no nativo de interés.
CL2012002634A 2010-03-31 2012-09-24 Microorganismo transgenico que comprende por lo menos un gen que codifica la glucosa-6-fosfato deshidrogenasa, por lo menos un gen que codifica 6-fosfogluconolactonasa y por lo menos un gen heterologo que codifica un producto no nativo de interes; levadura oleaginosa transgenica; y metodo para la produccion de un producto no nativo de interes. CL2012002634A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US31947310P 2010-03-31 2010-03-31

Publications (1)

Publication Number Publication Date
CL2012002634A1 true CL2012002634A1 (es) 2013-07-12

Family

ID=44710117

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2012002634A CL2012002634A1 (es) 2010-03-31 2012-09-24 Microorganismo transgenico que comprende por lo menos un gen que codifica la glucosa-6-fosfato deshidrogenasa, por lo menos un gen que codifica 6-fosfogluconolactonasa y por lo menos un gen heterologo que codifica un producto no nativo de interes; levadura oleaginosa transgenica; y metodo para la produccion de un producto no nativo de interes.

Country Status (8)

Country Link
US (1) US20110244512A1 (es)
EP (1) EP2553107A2 (es)
JP (1) JP2013530679A (es)
CN (1) CN103189513A (es)
AU (1) AU2011235307A1 (es)
CA (1) CA2790053A1 (es)
CL (1) CL2012002634A1 (es)
WO (1) WO2011123407A2 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2824584A1 (en) 2010-12-30 2012-07-05 E. I. Du Pont De Nemours And Company Use of saccharomyces cerevisiae suc2 gene in yarrowia lipolytica for sucrose utilization
US20140322779A1 (en) * 2011-11-11 2014-10-30 Genomatica, Inc. Eukaryotic organisms and methods for increasing the availability of cytosolic acetyl-coa, and for the producing 1,3-butanediol
US9181568B2 (en) 2012-04-23 2015-11-10 Exxonmobil Research And Engineering Company Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases
WO2014100061A1 (en) 2012-12-21 2014-06-26 E. I. Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
WO2018102728A1 (en) * 2016-12-01 2018-06-07 Arkansas State University - Jonesboro Method of improving chloroplast function and increasing seed yield
CN109929870B (zh) * 2019-02-20 2021-03-16 天津大学 糖代谢与脂质代谢协同提高解脂耶氏酵母合成脂肪酸衍生物的产量的应用
CN110699310B (zh) * 2019-11-05 2021-05-28 无锡晶扬生物科技有限公司 一种四氢嘧啶高产谷氨酸棒杆菌及其应用
CN111454854B (zh) * 2020-05-02 2022-05-06 昆明理工大学 一株产虾青素的红冬孢酵母基因工程菌株
CN113736677B (zh) * 2021-09-09 2023-02-28 陕西海斯夫生物工程有限公司 高产生育三烯酚的重组解脂亚罗酵母、其构建方法及应用
CN114480154B (zh) * 2022-01-20 2024-03-19 江苏大学 一种重组毕赤酵母工程菌及其构建方法与应用
CN114574373B (zh) * 2022-03-29 2022-11-01 陕西海斯夫生物工程有限公司 一株产生育酚的重组裂殖壶菌、其构建方法及应用
CN115851779B (zh) * 2022-10-29 2024-03-26 昆明理工大学 一种葡萄糖-6-磷酸脱氢酶基因RkZWF1及其应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4841093B2 (ja) * 1999-07-23 2011-12-21 アーカー−ダニエルズ−ミッドランド カンパニー 細胞性ｎａｄｐｈの増加によるｌ−アミノ酸の生成方法
US7125672B2 (en) * 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) * 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
FR2862068B1 (fr) * 2003-11-06 2007-10-12 Metabolic Explorer Sa Souches de microorganismes optimisees pour des voies de biosyntheses consommatrices de nadph
JP4670811B2 (ja) * 2004-02-25 2011-04-13 味の素株式会社 Ｌ−アミノ酸の生産方法
RU2009134794A (ru) * 2007-02-19 2011-03-27 Эвоник Дегусса ГмБх (DE) Способ получения метионина с использованием коринебактерий посредством свехэкспрессии ферментов пентозофосфатного пути
EP2615164A1 (en) * 2007-11-10 2013-07-17 Joule Unlimited Technologies, Inc. Hyperphotosynthetic organisms

Also Published As

Publication number Publication date
EP2553107A2 (en) 2013-02-06
CN103189513A (zh) 2013-07-03
AU2011235307A1 (en) 2012-08-23
CA2790053A1 (en) 2011-10-06
WO2011123407A2 (en) 2011-10-06
US20110244512A1 (en) 2011-10-06
WO2011123407A3 (en) 2013-05-30
JP2013530679A (ja) 2013-08-01

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