CA3218518A1 - Systeme et procede de microscopie faisant appel a un liquide d'immersion - Google Patents
Systeme et procede de microscopie faisant appel a un liquide d'immersion Download PDFInfo
- Publication number
- CA3218518A1 CA3218518A1 CA3218518A CA3218518A CA3218518A1 CA 3218518 A1 CA3218518 A1 CA 3218518A1 CA 3218518 A CA3218518 A CA 3218518A CA 3218518 A CA3218518 A CA 3218518A CA 3218518 A1 CA3218518 A1 CA 3218518A1
- Authority
- CA
- Canada
- Prior art keywords
- liquid
- oil
- objective lens
- immersion objective
- liquid immersion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000007654 immersion Methods 0.000 title claims abstract description 144
- 239000007788 liquid Substances 0.000 title claims abstract description 107
- 238000000034 method Methods 0.000 title claims abstract description 60
- 238000000386 microscopy Methods 0.000 title claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000003921 oil Substances 0.000 claims description 307
- 230000007246 mechanism Effects 0.000 claims description 22
- 238000012545 processing Methods 0.000 claims description 16
- 238000003860 storage Methods 0.000 claims description 8
- 238000003384 imaging method Methods 0.000 claims description 7
- 239000004215 Carbon black (E152) Substances 0.000 claims description 4
- 229930195733 hydrocarbon Natural products 0.000 claims description 4
- 150000002430 hydrocarbons Chemical class 0.000 claims description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 3
- 230000004044 response Effects 0.000 claims description 3
- 239000010703 silicon Substances 0.000 claims description 3
- 229910052710 silicon Inorganic materials 0.000 claims description 3
- 230000008569 process Effects 0.000 abstract description 19
- 230000008859 change Effects 0.000 abstract description 8
- 239000000523 sample Substances 0.000 description 67
- 230000003287 optical effect Effects 0.000 description 34
- 230000033001 locomotion Effects 0.000 description 22
- 230000008878 coupling Effects 0.000 description 19
- 238000010168 coupling process Methods 0.000 description 19
- 238000005859 coupling reaction Methods 0.000 description 19
- 238000005286 illumination Methods 0.000 description 16
- 239000012530 fluid Substances 0.000 description 15
- 239000012472 biological sample Substances 0.000 description 11
- 239000000463 material Substances 0.000 description 11
- 238000005303 weighing Methods 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 10
- 238000007373 indentation Methods 0.000 description 9
- 238000004891 communication Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 239000007850 fluorescent dye Substances 0.000 description 6
- 239000011888 foil Substances 0.000 description 6
- 239000011159 matrix material Substances 0.000 description 5
- 230000007480 spreading Effects 0.000 description 5
- 238000003892 spreading Methods 0.000 description 5
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- 230000005291 magnetic effect Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000002093 peripheral effect Effects 0.000 description 4
- 238000005476 soldering Methods 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 230000005670 electromagnetic radiation Effects 0.000 description 3
- 230000005284 excitation Effects 0.000 description 3
- 230000005294 ferromagnetic effect Effects 0.000 description 3
- 230000000284 resting effect Effects 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003302 ferromagnetic material Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000002952 image-based readout Methods 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 239000010959 steel Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- STECJAGHUSJQJN-USLFZFAMSA-N LSM-4015 Chemical compound C1([C@@H](CO)C(=O)OC2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-USLFZFAMSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
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- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 102000034287 fluorescent proteins Human genes 0.000 description 1
- 108091006047 fluorescent proteins Proteins 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000013537 high throughput screening Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000003129 oil well Substances 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/33—Immersion oils, or microscope systems or objectives for use with immersion fluids
Landscapes
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
L'invention concerne un dispositif de microscopie apte à (i) déplacer une lentille d'objectif à immersion dans un liquide vers un emplacement au niveau duquel un liquide, tel que de l'huile ou de l'eau, peut être appliqué à la lentille d'objectif à immersion dans un liquide, et à (ii) déplacer la lentille d'objectif à immersion dans un liquide sur laquelle est appliqué un liquide vers un porte-échantillon, à des fins d'utilisation pour une acquisition d'images d'un échantillon. Le procédé d'application de liquide peut être automatisé de sorte que le liquide appliqué sur la lentille d'objectif soit réapprovisionné si nécessaire. Le dispositif peut focaliser automatiquement la lentille pour faciliter le balayage, et peut être conçu pour changer des lentilles d'objectif. D'autres modes de réalisation, notamment l'utilisation d'un tel dispositif dans un balayage à haute teneur/à haut débit, sont également décrits.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163180694P | 2021-04-28 | 2021-04-28 | |
| US202163180693P | 2021-04-28 | 2021-04-28 | |
| US63/180,693 | 2021-04-28 | ||
| US63/180,694 | 2021-04-28 | ||
| PCT/IB2022/053972 WO2022229906A1 (fr) | 2021-04-28 | 2022-04-28 | Système et procédé de microscopie faisant appel à un liquide d'immersion |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3218518A1 true CA3218518A1 (fr) | 2022-11-03 |
Family
ID=82115765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3218518A Pending CA3218518A1 (fr) | 2021-04-28 | 2022-04-28 | Systeme et procede de microscopie faisant appel a un liquide d'immersion |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP4330753A1 (fr) |
| JP (1) | JP2024516261A (fr) |
| CA (1) | CA3218518A1 (fr) |
| IL (1) | IL297882A (fr) |
| WO (1) | WO2022229906A1 (fr) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4328359B2 (ja) * | 2007-01-15 | 2009-09-09 | オリンパス株式会社 | 液浸対物レンズを備えた観察装置 |
-
2022
- 2022-04-28 CA CA3218518A patent/CA3218518A1/fr active Pending
- 2022-04-28 JP JP2023566966A patent/JP2024516261A/ja active Pending
- 2022-04-28 EP EP22731787.2A patent/EP4330753A1/fr active Pending
- 2022-04-28 IL IL297882A patent/IL297882A/en unknown
- 2022-04-28 WO PCT/IB2022/053972 patent/WO2022229906A1/fr active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| IL297882A (en) | 2023-01-01 |
| JP2024516261A (ja) | 2024-04-12 |
| WO2022229906A1 (fr) | 2022-11-03 |
| EP4330753A1 (fr) | 2024-03-06 |
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